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Journal articles on the topic 'Sterilizing agents'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Hardjo, Popy Hartatie, and Alfian Hendra Krisnawan. "Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture." MPI (Media Pharmaceutica Indonesiana) 3, no. 1 (2020): 19–26. http://dx.doi.org/10.24123/mpi.v3i1.2836.

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The success of culture initiation depends on explant surface sterilization techniques. Suitable concentration, combinations, and duration of exposure of sterilizing agents are important to raise in vitro culture successfully. The aim of this work is to obtain the suitable sterilization method for explant buds of red ginger rhizome to get the axenic culture. Four sterilizing agents, fungicide, bactericide, Cefotaxime antibiotic, and NaOCl were tested for sterilization by various concentration and duration of exposure. The results showed that sterilizing agents 200 mg/L Cefotaxime and 100 mg/L B

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Amantayev, Nurbolat Gabdullaevich, Marina Mikhailovna Silantyeva, and Vadim Tagirovich Khassanov. "Selecting sterilization conditions for the explants of different potato varieties to be in vitro introduced into the culture." Bulletin of the Karaganda University. “Biology, medicine, geography Series” 111, no. 3 (2023): 16–24. http://dx.doi.org/10.31489/2023bmg3/16-24.

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An explant is a fragment of plant tissue or organ that is incubated on its own or used to produce a primary callus. The introduction of any plant’s tissues into the culture in vitro begins with the selection of an uninfected viable explant. The authors presented the results of the effect of various sterilizing agents as well as the treatment timing on the viability of plant explants during microclonal propagation of potatoes of the following varieties: Aladin, Gala, Nevsky, Udacha and Kostanay Novosti. The meristem sprouts from potato tubers were used as parent material. The following commerci

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Belete, Yonas Zeslase, and Hagos Kalu. "Protocol Optimization for Surface Sterilization of Sugarcane Variety (B-52/298)." International Letters of Chemistry, Physics and Astronomy 48 (March 2015): 50–60. http://dx.doi.org/10.18052/www.scipress.com/ilcpa.48.50.

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The aim of this study was to optimize conditions for surface sterilization of ex-plants of B-52/298 sugarcane cultivar variety. Experiment was carried out to determine the optimum concentrations of surface sterilizing agents and at what time they optimally sterilize. This experiment was conducted at MIT Plant Tissue Culture and Micro-propagation Laboratory Center, situated in Mekelle City. Three chemisterilants (Sodium Hypochlorite, Mercuric Chloride and Ethanol) were taken for the investigation. Three of the sterilizing agents were used in different concentrations at various time intervals an

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Belete, Yonas Zeslase, and Hagos Kalu. "Protocol Optimization for Surface Sterilization of Sugarcane Variety (B-52/298)." International Letters of Chemistry, Physics and Astronomy 48 (March 25, 2015): 50–60. http://dx.doi.org/10.56431/p-ta6564.

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The aim of this study was to optimize conditions for surface sterilization of ex-plants of B-52/298 sugarcane cultivar variety. Experiment was carried out to determine the optimum concentrations of surface sterilizing agents and at what time they optimally sterilize. This experiment was conducted at MIT Plant Tissue Culture and Micro-propagation Laboratory Center, situated in Mekelle City. Three chemisterilants (Sodium Hypochlorite, Mercuric Chloride and Ethanol) were taken for the investigation. Three of the sterilizing agents were used in different concentrations at various time intervals an

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Hashim, Saiyidah Nafisah, Siti Zulaiha Ghazali, Norrizah Jaafar Sidik, Tay Chia-Chay, and Azani Saleh. "Surface sterilization method for reducing contamination of Clinacanthus nutans nodal explants intended for in-vitro culture." E3S Web of Conferences 306 (2021): 01004. http://dx.doi.org/10.1051/e3sconf/202130601004.

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Surface sterilization is a vital step in preparation of healthy and viable explants in tissue culture. Most surface contaminants can be eliminated by surface sterilization with a suitable sterilizing agent. The study aimed to present an effective disinfection method for Clinacanthus nutans shoot regeneration using nodal segments. A total of four different sterilization approaches were conducted by treating nodal explants with various concentrations of sterilizing agent. Sterilizing agents used were Rhizophora apiculata Pyroligneous acid (PA), sodium hypochlorite (Clorox) thiophanate-methyl (fu

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MARČANOVÁ, Z., Z. KŘÍŽKOVÁ, and J. ŠAVEL. "Modern cleaning and sterilizing agents from PRAGOCHEMA Plant, Uhříněves." Kvasny Prumysl 37, no. 2 (1991): 41–43. http://dx.doi.org/10.18832/kp1991007.

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Neshumaeva, N. A., and M. A. Timina. "Selection of methods for sterilization of C. purpurea sclerotia for isolation of rye biosecurity agents." IOP Conference Series: Earth and Environmental Science 839, no. 4 (2021): 042010. http://dx.doi.org/10.1088/1755-1315/839/4/042010.

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Abstract Winter rye is a traditionally cultivated grain crop that is susceptible to ergot, a serious disease of the Poaceae family, caused by C. purpurea. Along with agrotechnical, organizational, and economic methods, biological control of ergot in grain crops is one of the ways to combat this disease. To isolate C. purpurea hyperparasites in a pure culture, the optimal sterilization options were selected, which were to exclude the infection of ergot sclerotia with saprophytic microflora. Before sterilization, C. purpurea were additionally washed in the running water with the addition of SAS

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Mihaljevic, Ines, Krunoslav Dugalic, Vesna Tomas, et al. "In vitro sterilization procedures for micropropagation of ‘oblacinska’ sour cherry." Journal of Agricultural Sciences, Belgrade 58, no. 2 (2013): 117–26. http://dx.doi.org/10.2298/jas1302117m.

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Surface sterilization is the most important step in preparation of explants for micropropagation, because controlling fungal and bacterial contamination of woody plant from field sources is very difficult. Six sterilizing agents: sodium hypochlorite (NaOCl), calcium hypochlorite [Ca(ClO)2], sodium dichloroisocyanurate (DICA), mercuric (II) chloride (HgCl2), silver nitrate (AgNO3) and hydrogen peroxide (H2O2) were tested for sterilization of ?Oblacinska? sour cherry buds, by varying their concentration and time of exposure. The aim of this study was to establish best surface sterilization for i

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Goktas, Fatih Mehmet, Bilgesu Sahin, and Sibel Yigitarslan. "Production of Sterilizing Agents fromCalendula officinalisExtracts Optimized by Response Surface Methodology." International Journal of Analytical Chemistry 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/789732.

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The aim of this study was to produce hand sterilizing liquid and wet wipes with the extracts ofCalendula officinalis. Since this plant has well known antimicrobial activity due to its phytochemical constituents, the increase in the extraction yield was chosen as the principle part of the production process. To achieve the maximum yield, parameters of solid-to-liquid ratio, extraction temperature, and time were studied. The optimum conditions were determined by response surface methodology as 41°C, 7 h, and 3.3 g/200 mL for temperature, time, and solid-to-liquid ratio, respectively. The yield a

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Mazzola, Priscila Gava, Angela Faustino Jozala, Letícia Célia de Lencastre Novaes, Patricia Moriel, and Thereza Christina Vessoni Penna. "Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents." Brazilian Journal of Pharmaceutical Sciences 45, no. 2 (2009): 241–48. http://dx.doi.org/10.1590/s1984-82502009000200008.

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Due to the growing number of outbreaks of infection in hospital and nurseries, it becomes essential to set up a sanitation program that indicates that the appropriate chemical agent was chosen for application in the most effective way. Validating the effectiveness of decontamination and disinfection is an important and often challenging task. In order to study and compare the behavior of selected microorganisms, they were submitted to minimal inhibitory concentration (MIC). The MIC intervals, which reduced bacteria populations over 6 log10, were: 59 to 156 mg/L of quaternary ammonium compounds

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González, María E., Carolina Lunati, Mirtha Floccari, and Elda M. Salmoral. "Effects of Sterilizing Agents on the Biodegradation of a Bioplastic Material." International Journal of Polymeric Materials 58, no. 3 (2009): 129–40. http://dx.doi.org/10.1080/00914030802583726.

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Khromova, T. M., and О. V. Matsneva. "Optimization of berry crop genotype regeneration systems at the in vitro crop initiation stage." BIO Web of Conferences 47 (2022): 04004. http://dx.doi.org/10.1051/bioconf/20224704004.

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The article presents data obtained during the study of the effectiveness of the initial stage of in vitro introduction of berry crops into culture: black currant (Ribes nigrum L.) and pine strawberry (Fragaria × ananassa Duch.) depending on the period of implementation and sterilizing agents used. The objects of research are varieties of black currant (Azhurnaya, Orlovskaya Serenada, Ocharovanie, Chudnoye Mgnovenye); pine strawberry (Asia, Alba, Bereginya, Darselekt, Kimberly, Klery, Marmolada Onebor, Siria, Honeoye, Florence, Frida, Tsaritsa, Urozhajnaya CGL). Introduction into culture in vit

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Halebathi, Ramesh Giriyappa, Preeti Karan Dodwad, Veerendra M. Uppin, Bhavana Gandhi, Priyanka Sarangi, and Neetika Sahni. "Comparative Evaluation of the Efficacy of Two Methods of Sterilization for Rotary Nickel-Titanium Files: An in vitro Study." World Journal of Dentistry 2, no. 3 (2011): 193–98. http://dx.doi.org/10.5005/jp-journals-10015-1082.

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ABSTRACT Sterilization in endodontic practice is a must as the major motive of an endodontist is to combat endodontic diseases by eliminating the causative microorganisms. Owing to the design of the endodontic files, it is very conducive for the microorganisms and debris to be anchored onto them. So, the aim of this study was to investigate the sterilizing efficacy of two methods of sterilization using rotary nickeltitanium files: Autoclave and chemical sterilization using two solutions (Quitanet Plus and GlutraMil) and to check whether any sterilizing technique could match the standard of aut

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Otieno Oginga, Fredrick, Felisters Akal Edung, Vasco Dominic Kulimankudya, Brenda Lijodi, Jeremiah Motari, and Charles Stephen Okila. "Advancements in HIV/AIDS Cure Research: Effects on Patient Adherence and Behavioral Trends in the General Population a Scoping Review." International Journal of Research and Scientific Innovation XI, no. X (2024): 355–68. http://dx.doi.org/10.51244/ijrsi.2024.1110029.

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Introduction: Despite significant global efforts, HIV/AIDS remains a major health issue, with over 39 million people living with HIV worldwide (UNAIDS, 2024). Although Antiretroviral Therapy (ART) has improved management, a definitive cure remains elusive. Recent advancements in HIV cure research include functional and sterilizing cures. Functional cures aim to suppress the virus without daily ART, while sterilizing cures seek complete eradication. Breakthroughs in gene editing, stem cell transplants, and latency-reversing agents (LRAs) offer promise but face challenges. Research Gap: The effe

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B.M., Bhyravi, Athani S.I., D. P. Prakasha, et al. "Effect of sterilizing agent and seed treatment method on In vitro seed germination of guava cv. Arka Kiran." Ecology, Environment and Conservation 30, no. 02 (2024): 588–91. http://dx.doi.org/10.53550/eec.2024.v30i02.028.

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The in vitro germination of seeds of guava cv. Arka Kiran was mainly affected by contamination and seed coat dormancy. For successful in vitro germination of guava seeds, contamination control and breaking of seed dormancy are the two main steps one should fallow. An experiment on “Effect of sterilizing agent and seed treatment method on in vitro seed germination of guava cv. Arka Kiran” was carried out at College of Horticulture, Sirsi, Uttar Kannada, Karnataka during 2021-2023. The study revealed significant influence of different sterilizing agents and seed treatment method on contamination

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Neeraj, Singh, K. Nandmer A, K. Nandmer V, and Dube Simmi. "Role of Bowel Wash and Gut Sterilizing Agents in Recovery from Hepatic Encephalopathy." International Journal of Pharmaceutical and Clinical Research 16, no. 7 (2024): 615–21. https://doi.org/10.5281/zenodo.12804728.

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<strong>Background:</strong> Hepatic encephalopathy (HE) is a severe neurological complication of liver dysfunction, frequently seen in patients with cirrhosis or acute liver failure. Bowel wash and gut sterilizing agents have emerged as potential treatments to alleviate HE symptoms by targeting gut-derived neurotoxins, such as ammonia. <strong>Aim and Objectives:</strong> To evaluate the effectiveness of bowel wash and gut sterilizing agents in improving clinical outcomes and reducing recovery time in patients with hepatic encephalopathy. <strong>Materials and Methods:&nbs

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Nishant, Bhagwat S. Sharma, Shilpi Singh Chandel, and Neha Rajput. "A Comparative Analysis of Extracted Human Teeth Sterilization by Using Distinctive Sterilizing Agents." Journal of College of Medical Sciences-Nepal 20, no. 1 (2024): 84–86. http://dx.doi.org/10.3126/jcmsn.v20i1.59077.

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Background Regular use for practice and educational purposes of Extracted human teeth in dental institutions at undergraduate and postgraduate levels is a necessity. Individuals who collect extracted teeth must decontaminate and then handle teeth. Different materials and methods are used for sterilizing extracted teeth to avoid infection from them.MethodsTotal of 50 freshly extracted human teeth that were intact, non-carious and extracted due to periodontal disease or orthodontic purpose were divided randomly into 5 groups consisting of 10 teeth in each group.These were stored in sterile salin

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NAGASUPRIYA, T. VEERA, K. VENKATA LAXMI, P. SAIDAIAH, VEENA JOSHI, and M. GOPALA KRISHNA. "EFFECT OF DIFFERENT SURFACE STERILIZATION METHODS AND DIFFERENT PLANT GROWTH REGULATORS ON CULTURE ESTABLISHMENT FROM POMEGRANATE NODAL EXPLANTS OF cv. BHAGWA." Asian Journal of Microbiology, Biotechnology & Environmental Sciences 26, no. 04 (2024): 486–91. https://doi.org/10.53550/ajmbes.2024.v26i04.007.

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The present investigation was conducted to know the effect of different surface sterilization methods and different plant growth regulators on culture establishment from Pomegranate nodal explants of cv. Bhagwa with two factors: Sterilizing agents(S): 0.1% Mercuric chloride (HgCl2) and 1% Sodium hypochlorite (NaOCl) each at two different timing intervals i.e., 5 min and 10 min and growth regulators(G) G1: BAP 2 mg L-1, G2: Kinetin 2 mg L-1, G3: 2-ip 2 mg L-1, G4: zeatin 2 mg L-1). The results revealed that highest percentage of uncontamination was recorded in S2: 0.1% HgCl2 for 10min (93.33%)

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Navarro-Llopis, V., J. Sanchis, J. Primo-Millo, and E. Primo-Yúfera. "Chemosterilants as control agents of Ceratitis capitata (Diptera: Tephritidae) in field trials." Bulletin of Entomological Research 97, no. 4 (2007): 359–68. http://dx.doi.org/10.1017/s0007485307005081.

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AbstractLufenuron is a chitin synthesis inhibitor, which is able to impede Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reproduction. In laboratory trials, following ingestion of lufenuron, the eggs laid by female Ceratitis capitata were prevented from hatching. In field trials in Valencia, Spain, lufenuron showed its effectiveness by reducing C. capitata wild populations and its continuous application to several generations of fruit fly resulted in increased pest control. This field trial was conducted in an isolated valley some 80 ha in size, over a continuous four-year period. I

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Markova, Marina, and Elena Somova. "Regeneration capacity of Cerasus fruticosa and Prunus domestica into the in vitro culture." Agrarian Bulletin of the 209, no. 06 (2021): 43–52. http://dx.doi.org/10.32417/1997-4868-2021-209-06-43-52.

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Abstract. The aim of these studies was to introduce into the in vitro culture the steppe cherry (Cerasus fruticosa) variety Shchedraya and the domestic plum (Prunus domestica) variety Sineokaya for subsequent micropropagation. Methods. Optimal conditions for obtaining viable explants, such as sterilizing agent and initiation time, have been investigated. The suitability of various nutrient media for in vitro cultivation of these cultures has also been tested. As a result of the experiments, it was revealed that the most effective sterilizing agents were 38 % perhydrol (control) and 6% chlorhex

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Lal, Manmohan, Mahital Jamwal, Parshant Bakshi, et al. "Establishing surface sterilization protocol for clonal apple rootstock MM106." Journal of Applied and Natural Science 15, no. 4 (2023): 1654–59. http://dx.doi.org/10.31018/jans.v15i4.5147.

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Surface sterilization is crucial in preparing viable and uncontaminated explants for tissue culture. Utilizing the right sterilizing agent in this procedure can efficiently eliminate the majority of surface contaminants. The present study aimed to establish the best sterilization procedure for clonal apple rootstock MM106 using shoot tips and nodal segments. Two important sterilizing agents, mercuric chloride (ranging from 0.05% to 0.3%) for various durations (2 to 6 minutes) and sodium hypochlorite (ranging from 3.0% to 12%) for different durations (10 to 30 minutes), were employed. The resul

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Fedotov, Yu A., K. A. Timakova, A. V. Tarasov, S. A. Lepeshin, and Ye S. Yavorskaya. "Polymer Membranes in Medicine and Pharmaceutics." Eurasian Chemico-Technological Journal 14, no. 2 (2012): 147. http://dx.doi.org/10.18321/ectj108.

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This review covers the main problems and basic principles of the use of various membranes in medicine and pharmacy. Developments in the field of sterilizing, bacteriostatic and bactericidal membranes and membranes for pyrogen-free water preparing are considered. An analysis of the scientific literature in this research area are identified. The main ways of development of this area, as well as an analysis of properties of membranes from different manufacturers for use in the medical and pharmaceutical industries are given. Their advantages and disadvantages are considered. It is shown that the

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Loraine, Jessica, Feifei Pu, Obolbek Turapov, and Galina V. Mukamolova. "Development of anIn VitroAssay for Detection of Drug-Induced Resuscitation-Promoting-Factor-Dependent Mycobacteria." Antimicrobial Agents and Chemotherapy 60, no. 10 (2016): 6227–33. http://dx.doi.org/10.1128/aac.00518-16.

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ABSTRACTTuberculosis is a major infectious disease that requires prolonged chemotherapy with a combination of four drugs. Here we present data suggesting that treatment ofMycobacterium tuberculosis, the causative agent of tuberculosis, andMycobacterium smegmatis, a model organism widely used for the screening of antituberculosis agents, with first-line drugs resulted in the generation of substantial populations that could be recovered only by the addition of a culture supernatant from growing mycobacteria. These bacilli failed to grow in standard media, resulting in significant underestimation

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Knyazev, Aleksey, Bulat Kuluev, Alexander Fateryga, Gulnar Yasybaeva, and Aleksey Chemeris. "Aseptic Germination and Agrobacterium rhizogenesmediated Transformation of Taraxacum hybernum Steven." Plant Tissue Culture and Biotechnology 27, no. 2 (2017): 141–51. http://dx.doi.org/10.3329/ptcb.v27i2.35019.

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Taraxacum hybernum Steven, also known as krym‐saghyz, is one of the potential sources of natural rubber. The authors studied the aseptic germination of the T. hybernum seeds using various sterilizing agents and their effect on contamination. In vitro T. hybernum plants were used for Agrobacterium rhizogenesmediated transformation. A4 and 15834 strains of A. rhizogenes showed similar effectiveness after inoculation by injection into the hypocotyls. Authors generated the hairy roots of T. hybernum and the transgenic forms of this plant using A. rhizogenes‐mediated transformation.Plant Tissue Cul

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Falah Mahmood Hameed, Naseer A. Al-Arubaye, and Abeer Fadeel kadear. "A Review of Chemical Castration in Domestic Animals." Academic International Journal of Veterinary Medicine 2, no. 1 (2024): 43–47. http://dx.doi.org/10.59675/v216.

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This review article discusses the use of numerous chemical agents supplied through various routes to regulate the population of several animal species, including dogs, guinea pigs, rabbits, rams, and rats. While surgery is the safest and most effective procedure, it is also the most expensive. Therefore, non-surgical sterilization methods that would make male sterilization cheap, simple, and quick for sterilizing a large number of male animals in a short amount of time were introduced in an effort to slow down the increase in the animal population effectively.

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Meghwal, P. R., H. C. Sharma, and S. K. Singh. "Effect of surface sterilizing agents on in vitro culture establishment of guava (Psidium guajava L.)." Journal of Applied Horticulture 02, no. 02 (2000): 94–95. http://dx.doi.org/10.37855/jah.2000.v02i02.28.

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Natalchuk, T. A., T. V. Medvedieva, Ya. S. Zapolskyi, and O. B. Barban. "FEATURES OF INTRODUCTION OF SOUR CHERRIES VARIETY 'KSENIIA' AND CHERRIES VARIETY 'VASYLYSA PREKRASNA' INTO IN VITRO CULTURE." Plant varieties studying and protection 16, no. 1 (2020): 97–102. https://doi.org/10.21498/2518-1017.16.1.2020.201353.

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<strong>Purpose.</strong> Determine the optimal timing of explant selection, select sterilizing agents and sterilization regimens, as well as the nutrient medium for the introduction of new perspective varieties of sour cherries (<em>Prunus cerasus</em> L.) and cherries (<em>Prunus avium</em> L.) into <em>in vitro</em> culture.  <strong>Methods.</strong> During the work the method of clonal micropropagation of plants and statistical processing of experimental data were applied. <strong>Results.</strong> The optimal term of explants selection , the durati

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Wadhawan, Richa, Shikha Jhade, Kaunteya Kamad, Rishabh Bhargava, Sunny Tyagi, and Divya Bansal. "Pioneering Laser Applications In Modern Strategies For Sterilizing Periodontal Tissues." IOSR Journal of Dental and Medical Sciences 23, no. 9 (2024): 21–28. http://dx.doi.org/10.9790/0853-2309012128.

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The integration of laser technology into periodontal therapy represents a revolutionary leap forward in the management of periodontal diseases, as it provides a highly precise and minimally invasive alternative to traditional mechanical methods such as air turbines and ultrasonic scalers, which frequently cause significant patient discomfort. Lasers, with their advanced delivery systems—including articulated arms, hollow waveguides, and fiber optics—leverage mechanisms such as photo thermal and photomechanical ablation, as well as photochemical effects, aids in accomplishing improved bacterial

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Maslova, Elena, Natalya Gulya, Tatyana Perelugina, Valeria Semykina, and Elena Kalashnikova. "Introduction of hyssopus officinalis l . into in vitro culture to optimize the conditions for obtaining callus tissues and microclonal propagation as a promising metod of innovative agrobiotechnologies." BIO Web of Conferences 30 (2021): 05006. http://dx.doi.org/10.1051/bioconf/20213005006.

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The sterilization process of plant explants of H. officinalis was optimized when introduced into an in vitro culture, the most effective sterilization modes, optimal sterilizing agents, their exposure time and concentration were selected. Callus tissues and mini-plants of H. officinalis were obtained in vitro and the most optimal nutrient media were determined both for microclonal propagation and for the induction of callus tissue H. officinalis, which can be further used for mass cultivation of cell and culture and obtaining safe bio-additives with active substances for livestock and crop pro

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Lenaerts, Anne M., Sharon E. Chase, and Michael H. Cynamon. "Evaluation of Rifalazil in a Combination Treatment Regimen as an Alternative to Isoniazid-Rifampin Therapy in a Mouse Tuberculosis Model." Antimicrobial Agents and Chemotherapy 44, no. 11 (2000): 3167–68. http://dx.doi.org/10.1128/aac.44.11.3167-3168.2000.

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ABSTRACT The newer rifamycin, rifalazil (RLZ) (previously known as KRM-1648), has been shown in prior experiments to be a highly potent drug against Mycobacterium tuberculosis. In this report, we studied the efficacy of RLZ in combination with pyrazinamide (PZA) and ethambutol (EMB) in a long-term in vivo experiment and compared their activity with the isoniazid (INH)-rifampin (RIF) combination which is presently used in the clinic. Combinations of RLZ with PZA alone or with both PZA and EMB were both found to have sterilizing activities comparable to that of the INH-RIF combination but signif

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Bani Hani, Mohammed N., Abdel Rahman Al Manasra, Hamzeh Daradkah, Farah Bani Hani, and Zeina Bani Hani. "Cutaneous Myiasis Around Gastrostomy (PEG) Tube Insertion Site: The Second Case Report." Clinical Medicine Insights: Case Reports 12 (January 2019): 117954761986900. http://dx.doi.org/10.1177/1179547619869009.

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Background: Myiasis refers to the infestation of live human with 2-winged larvae (maggots). Cutaneous myiasis is the most commonly encountered clinical form. It is divided into 3 main forms: furuncular, creeping (migratory), and wound (traumatic) myiasis. Case report: In this article, we report an extremely rare case of myiasis around percutaneous endoscopic gastrostomy (PEG) tube in a 71-year-old female patient. She had the tube placed for feeding purposes, 8 months prior to her presentation. Family noticed alive worms emerging from skin at the gastrostomy tube insertion site. Patient was tre

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MARKOVA, M. G., and E. N. SOMOVA. "INITIATION OF HORTICULTURAL CROP EXPLANTS IN VITRO." Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, no. 4 (2022): 71–81. http://dx.doi.org/10.26897/0021-342x-2022-4-71-81.

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The stage of introduction into sterile culture plays an important role in clonal micropropagation. The purpose of these studies was to find the optimal sterilizing agent at the stage of introducing blue honeysuckle, red raspberry, garden strawberry, steppe cherry, house plum into in vitro culture, as well as the optimal nutrient medium at this stage for blue honeysuckle and red raspberry. As sterilizing agents with optimal exposure, the following were used: 48% ethyl alcohol (control) 8–10 min., 33% perhydrol 6–8 min., 6% chlorhexidine 8–10 min., 0.1% sublimate 1–2 min., 10.0% Domestos 6–7 min

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Emoghene, B. O., M. Idu, C. R. Eke, and O. Asemota. "Effects Of Different Sterilization Regimes & Growth Regulators On Micropropagation Of Female Date Palm (Phoenix dactylifera L.)." Nigerian Journal of Biotechnology 37, no. 1 (2020): 159–68. http://dx.doi.org/10.4314/njb.v37i1.17.

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The success of in vitro culture techniques is always hampered by microbial contamination. The present study was carried out to develop an efficient protocol for date palm explants sterilization for successful somatic embryos induction and plantlets formation of some date palm varieties. The shoot tips were treated with different sterilizing agents at different concentrations and durations of exposure. The use of ethanol (70%), sodium hypochlorite (3.5% & 70%) and mercuric chloride (0.2%) with or without addition of Tween-20 had different effects on decontamination of the date palm explants

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Sengupta, Pallav, and Sulagna Dutta. "Chemosterilization in Male: ‘Past And Present’ in Reproductive Biology." Biomedical and Pharmacology Journal 15, no. 1 (2022): 1–4. http://dx.doi.org/10.13005/bpj/2336.

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The effects of chemosterilizing agents on the testes and sperm production and maturation are much less understood than their effects on the ovaries and oogenesis. It was discovered that sterilising male insects with ionising radiation caused the emergence of chromosome-wide or chromatid-specific dominant lethal mutations, which led to the development of the classical male castration techniques1, 2. It was discovered in those investigations that alterations were not frequently found on spermatozoa, but that they were commonly visible in anomalies during the division of the zygote in the fertili

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Глибовець, А. О., С. О. Лашук, and Л. М. Мазурець. "Examining impact of sterilizing agents, growth regulators, carbohydrates and gelling agents on regeneration of fullfeatured plant of hops in micro-clonal multiplication." Plant varieties studying and protection, no. 3(17) (December 20, 2012): 74–76. http://dx.doi.org/10.21498/2518-1017.3(17).2012.58841.

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Mazzola, Priscila Gava, Angela Faustino Jozala, Letícia Célia de Lencastre Novaes, Patricia Moriel, and Thereza Christina Vessoni Penna. "Choice of sterilizing/disinfecting agent: determination of the Decimal ReductionTime (D-Value)." Brazilian Journal of Pharmaceutical Sciences 45, no. 4 (2009): 701–8. http://dx.doi.org/10.1590/s1984-82502009000400013.

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Efforts to diminish the transmission of infections include programs in which disinfectants play a crucial role. Hospital surfaces and medical devices are potential sources of cross contamination, and each instrument, surface or area in a health care unit can be responsible for spread of infection. The decimal reduction time was used to study and compare the behavior of selected strains of microorganisms. The highest D-values for various bacteria were obtained for the following solutions: (i) 0.1% sodium dichloroisocyanurate (pH 7.0) - E. coli and A. calcoaceticus (D = 5.9 min); (ii) sodium hyp

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Williams, Kathy, Austin Minkowski, Opokua Amoabeng, et al. "Sterilizing Activities of Novel Combinations Lacking First- and Second-Line Drugs in a Murine Model of Tuberculosis." Antimicrobial Agents and Chemotherapy 56, no. 6 (2012): 3114–20. http://dx.doi.org/10.1128/aac.00384-12.

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ABSTRACTNovel oral regimens composed of new drugs with potent activity againstMycobacterium tuberculosisand no cross-resistance with existing agents are needed to shorten and simplify treatment for both drug-susceptible and drug-resistant tuberculosis. As part of a continuing effort to evaluate novel drug combinations for treatment-shortening potential in a murine model, we performed two long-term, relapse-based experiments. In the first experiment, several 3- and 4-drug combinations containing new agents currently in phase 2/3 trials (TMC207 [bedaquiline], PA-824 and PNU-100480 [sutezolid], a

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Tasneen, Rokeya, Fabrice Betoudji, Sandeep Tyagi, et al. "Contribution of Oxazolidinones to the Efficacy of Novel Regimens Containing Bedaquiline and Pretomanid in a Mouse Model of Tuberculosis." Antimicrobial Agents and Chemotherapy 60, no. 1 (2015): 270–77. http://dx.doi.org/10.1128/aac.01691-15.

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ABSTRACTNew regimens based on two or more novel agents are sought to shorten or simplify treatment of tuberculosis (TB). Pretomanid (PMD) is a nitroimidazole in phase 3 trials that has significant bactericidal activity alone and in combination with bedaquiline (BDQ) and/or pyrazinamide (PZA). We previously showed that the novel combination of BDQ+PMD plus the oxazolidinone sutezolid (SZD) had sterilizing activity superior to that of the first-line regimen in a murine model of TB. The present experiments compared the activity of different oxazolidinones in combination with BDQ+PMD with or witho

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Макаров, С. С., Е. И. Куликова, А. М. Антонов, И. Б. Кузнецова, and Е. А. Сурина. "PECULIARITIES OF CLONAL MICROPROPAGATION OF CLOUDBERRY (RUBUS CHAMAEMORUS L.) USING VARIOUS STERILIZING AGENTS AND GROWTH-REGULATING SUBSTANCES." Вестник Бурятской государственной сельскохозяйственной академии имени В. Р. Филиппова, no. 2(71) (June 29, 2023): 118–25. http://dx.doi.org/10.34655/bgsha.2023.71.2.015.

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В статье приведены результаты исследований по изучению влияния стерилизующих агентов на жизнеспособность эксплантов и состава питательных сред MS, QL и концентраций росторегулирующих веществ 6-БАП, 2-iP на органогенез микрорастений морошки приземистой in vitro. В мире имеется спрос на ягодную продукцию морошки в связи с высокой пищевой и лекарственной ценностью. Морошка приземистая способна успешно произрастать на выработанных торфяниках. Создание специализированных плантаций на выработанных торфяниках может способствовать восстановлению зарослей и повышению урожайности морошки. Для получения

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Moisan, Michel, Jean Barbeau, Marie-Charlotte Crevier, Jacques Pelletier, Nicolas Philip, and Bachir Saoudi. "Plasma sterilization. Methods and mechanisms." Pure and Applied Chemistry 74, no. 3 (2002): 349–58. http://dx.doi.org/10.1351/pac200274030349.

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Utilizing a plasma to achieve sterilization is a possible alternative to conventional sterilization means as far as sterilization of heat-sensitive materials and innocuity of sterilizing agents are concerned. A major issue of plasma sterilization is the respective roles of ultraviolet (UV) photons and reactive species such as atomic and molecular radicals. At reduced gas pressure (£10 torr) and in mixtures containing oxygen, the UV photons dominate the inactivation process, with a significant contribution of oxygen atoms as an erosion agent. Actually, as erosion of the spore progresses, the nu

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Zaytseva, Svetlana M., Elena A. Kalashnikova, Rima N. Kirakosyan, et al. "Features of obtaining in vitro cultures of relict medicinal plants with a high content of biologically active substances (Taxus canadensis Marsh, Ginkgo biloba, Sequoia sempervirens L., Dioscorea caucasia Lypsky)." Veterinariya, Zootekhniya i Biotekhnologiya 5, no. 114 (2023): 68–77. http://dx.doi.org/10.36871/vet.zoo.bio.202305009.

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The effect of the biochemical status of plants on the viability of explants and obtaining a productive culture of medicinal plants in vitro, taking into account the use of various sterilizing agents and photoperiod, was studied. It was found that the applied lighting mode had a significant effect on the intensity of callus tissue formation, its consistency and color. As a rule, callus tissue was formed in those places where the localization of phenolic compounds was insignificant. When calluses are initiated on explants characterized by a high content of polyphenols, they are released into the

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Kulikova, Elena, Sergey Makarov, Irina Kuznetsova, and Anton Chudetsky. "Russian and Foreign Cultivars of Honeysuckle (Lonicera edulis Turcz.): cultivation studies in vitro." Food Processing: Techniques and Technology 51, no. 4 (2022): 712–22. http://dx.doi.org/10.21603/2074-9414-2021-4-712-722.

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Introduction. The demand for honeysuckle berries and planting material is growing. Clonal micropropagation is the most effective method for industrial plantations. The research objective was to study the effect of cytokinins and auxins on Russian and Canadian honeysuckle microshoots and roots. Study objects and methods. The study featured regenerated honeysuckle (Lonicera edulis Turcz.) of three Russian cultivars (Bakcharsky Velikan, Doch Velikana, Yugana) and two Canadian cultivars (Boreal Beauty, Boreal Beast). The experiment focused on the effect of sterilizing agents and sterilizatio

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Makarov, Sergey, Mikhail Upadyshev, Natalia Sungurova, Olga Tyukavina, Elena Kulikova, and Irina Kuznetsova. "Clonal Micropropagation of Wild Berry Plants of the Genus Rubus." Food Processing: Techniques and Technology 54, no. 1 (2024): 60–70. http://dx.doi.org/10.21603/2074-9414-2024-1-2488.

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Cloudberry (Rubus chamaemorus L.) and arctic raspberry (Rubus arcticus L.) are highly nutritional and medicinal but lowyield berries, with some populations being on the verge of extinction. Micropropagation biotechnologies are cost-effective and may provide healthy and plentiful planting material for these valuable berries. Clonal micropropagation of cloudberry and arctic raspberry requires new methods adapted for Russian varieties. This research featured the effect of sterilizing agents on the survival rate of explants of R. chamaemorus and R. arcticus, as well as the effect of growth regulat

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Ali, Moazam, Misbah Ijaz, Asad Manzoor, et al. "Efficacy of Azadirachta indica (Neem) leaf extract and hypertonic saline solution as intratesticular chemical sterilizing agents in dogs." Brazilian Journal of Veterinary Research and Animal Science 57, no. 4 (2020): e171582. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2020.171582.

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Castration refers to induced sterility via physical, chemical, or hormonal methods. Chemical castration is an efficient and reliable technique in contrast to other sterilization procedures as it is less painful to physical methods and cost‑effective to hormonal methods. Azadirachta indica (neem), is a charismatic plant as its leaves possess anti-inflammatory, anti‑microbial, and anti-androgenic chattels. To abate the escalating human population in South Asia, neem oil and neem leaf extract have been effectively used as a contraceptive agent. The key determinant of the current study was to eval

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Oniang'o, Mary Opisa, Stafford Achwanya Oliver, Otieno Otaye Daniel, and Mburugu Muthamia Japheth. "The efficacy of sterilizing agents, copper oxychloride, vegetable oil and agrowipe (botanic neem extract) against crown gall disease of roses in Kericho, Kenya." African Journal of Biological Sciences 2, no. 4 (2020): 115–21. https://doi.org/10.33472/AFJBS.2.4.2020.115-121.

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The experiments were conducted at James Finlay Kenya, flowers division Tarakwet farm in Kericho county from January 2016 to December 2016 to study the efficacy of copper oxychloride 1.0 g/L, hydrogen peroxide 1.0 ml/L, dettol 0.5 mL/L and 1.0 mL/L, agrowipe undiluted and fresh fri undiluted as control agents of crown gall disease in roses. Crown gall causes losses of between 75-95% on susceptible rose varieties hence the need to develop effective control strategies. Results showed plots treated with agrowipe and fresh fri had higher yield of roses, which were of better quality, longer, heavier

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Tasneen, Rokeya, Kathy Williams, Opokua Amoabeng, et al. "Contribution of the Nitroimidazoles PA-824 and TBA-354 to the Activity of Novel Regimens in Murine Models of Tuberculosis." Antimicrobial Agents and Chemotherapy 59, no. 1 (2014): 129–35. http://dx.doi.org/10.1128/aac.03822-14.

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ABSTRACTNew regimens based on two or more novel agents are sought in order to shorten or simplify the treatment of both drug-susceptible and drug-resistant forms of tuberculosis. PA-824 is a nitroimidazo-oxazine now in phase II trials and has shown significant early bactericidal activity alone and in combination with the newly approved agent bedaquiline or with pyrazinamide with or without moxifloxacin. While the development of PA-824 continues, a potential next-generation derivative, TBA-354, has been discovered to havein vitropotency superior to that of PA-824 and greater metabolic stability

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Jumana Sadiq Hassan, Maryam Issa Hameed, Ahmed Ail Hassen, Batool Saleh Chuaied, Zainab Ahmed Sabir, and Mona Riyad Kamel. "STUDY OF THE EFFECT OF ETHANOL ALCOHOLS ON YEASTS AND FUNGI ISOLATED FROM THE NAIL." European Journal of Medical Genetics and Clinical Biology 1, no. 6 (2024): 40–47. http://dx.doi.org/10.61796/jmgcb.v1i6.557.

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The right to use alcoholic materials excessively, such as 75% ethanol concentration, which is produced locally by several companies, and the use of these materials as sterilizing agents to get rid of certain kinds of viruses or microorganisms, as happened recently due to the Corona virus outbreak, have resulted in an increase in resistance. This study, which involved bleaching a number of yeast species, including Candida albicans, Candida glabrata, Candida krusei, Candida tropicalis, Rhodotorula, and Aspergillus flavus, revealed the emergence of high resistance by those yeasts and fungi to the

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Макаров, С. С., Е. И. Куликова, И. Б. Кузнецова, Т. А. Макарова, and З. А. Самойленко. "THE USAGE OF MODERN STERILIZING AGENTS WHEN INTRODUCING FOREST BERRY PLANTS OF THE VACCINIUM GENUS INTO IN VITRO CULTURE." Вестник Бурятской государственной сельскохозяйственной академии имени В. Р. Филиппова, no. 3(72) (September 28, 2023): 87–94. http://dx.doi.org/10.34655/bgsha.2023.72.3.010.

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В статье приведены результаты исследований по клональному микроразмножению клюквы крупноплодной (Vaccinium macrocarpon Ait.) российских сортов и голубики топяной (Vaccinium uliginosum L.) форм северно-российского происхождения на этапе введения в культуру in vitro. Создание плантаций лесных ягодных растений на выработанных торфяниках будет способствовать удовлетворению спроса на ягодную продукцию. Для ускоренного получения высококачественного посадочного материала ягодных растений наиболее целесообразно применять метод клонального микроразмножения. В настоящее время при получении первичной ант

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Singh, Devendra, and R. V. Singh. "SYNTHETIC PATHWAYS, STRUCTURAL FEATURES AND BIOCHEMICAL APPLICATIONS AS STERILIZING AGENTS OF SOME DIPHENYLSILICON(IV) CHELATES WITH N⌢SH SYSTEM." Phosphorus, Sulfur, and Silicon and the Related Elements 61, no. 1-2 (1991): 57–64. http://dx.doi.org/10.1080/10426509108027337.

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Fedorova, Olga, N. Evtushenko, Petr Evlakov, Ol'ga Zaharova, and Alexander Gusev. "Use of copper oxide nanopreparations as sterilizing agents at the stage of introducing woody plants into in vitro culture." Forestry Engineering Journal 15, no. 2 (2025): 142–57. https://doi.org/10.34220/issn.2222-7962/2025.2/9.

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One of the main difficulties preventing the widespread introduction of clonal micropropagation technology to obtain high-quality planting material for woody plants is the difficulty of obtaining an aseptically pure culture with high regenerative capacity. The article discusses the effects of copper oxide nanoparticle solutions (75-100 nm) stabi-lized by nonionic surfactants (Tween 20, Triton X100 and Polyhexamethylenebiguanide) on the stage of introducing the Poplar × aspen hybrid and downy birch 15-1 into in vitro culture. It is assumed that the introduction of nanoparti-cles into the Murasig

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