Relevant bibliographies by topics / Stirred tank bioreactor

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
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  5. Conference papers

Academic literature on the topic 'Stirred tank bioreactor'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 February 2022

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Journal articles on the topic "Stirred tank bioreactor"

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Schirmer, Cedric, Rüdiger W. Maschke, Ralf Pörtner, and Dieter Eibl. "An overview of drive systems and sealing types in stirred bioreactors used in biotechnological processes." Applied Microbiology and Biotechnology 105, no. 6 (March 2021): 2225–42. http://dx.doi.org/10.1007/s00253-021-11180-7.

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AbstractNo matter the scale, stirred tank bioreactors are the most commonly used systems in biotechnological production processes. Single-use and reusable systems are supplied by several manufacturers. The type, size, and number of impellers used in these systems have a significant influence on the characteristics and designs of bioreactors. Depending on the desired application, classic shaft-driven systems, bearing-mounted drives, or stirring elements that levitate freely in the vessel may be employed. In systems with drive shafts, process hygiene requirements also affect the type of seal used. For sensitive processes with high hygienic requirements, magnetic-driven stirring systems, which have been the focus of much research in recent years, are recommended. This review provides the reader with an overview of the most common agitation and seal types implemented in stirred bioreactor systems, highlights their advantages and disadvantages, and explains their possible fields of application. Special attention is paid to the development of magnetically driven agitators, which are widely used in reusable systems and are also becoming more and more important in their single-use counterparts.Key Points• Basic design of the most frequently used bioreactor type: the stirred tank bioreactor• Differences in most common seal types in stirred systems and fields of application• Comprehensive overview of commercially available bioreactor seal types• Increased use of magnetically driven agitation systems in single-use bioreactors
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Kennes, Christian, María Montes, M. Estefanía López, and María C. Veiga. "Waste gas treatment in bioreactors: environmental engineering aspectsThis article is one of a selection of papers published in this Special Issue on Biological Air Treatment." Canadian Journal of Civil Engineering 36, no. 12 (December 2009): 1887–94. http://dx.doi.org/10.1139/l09-113.

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This paper gives an overview of the most important bioreactor configurations used in waste gas treatment, i.e., conventional and trickling biofilters, the bioscrubber, suspended-growth bioreactors, as well as two-liquid phase systems (stirred tank bioreactor and biotrickling filter). The historical evolution, main characteristics, and recent developments are described in each case.
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Fitzpatrick, John J. "Insights from Mathematical Modelling into Energy Requirement and Process Design of Continuous and Batch Stirred Tank Aerobic Bioreactors." ChemEngineering 3, no. 3 (July 13, 2019): 65. http://dx.doi.org/10.3390/chemengineering3030065.

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Bioreaction kinetics, oxygen transfer and energy modelling were applied to stirred tank aerobic bioreactors. This was done to investigate how key input design variables influence bioreactor size, feed and wasted substrate, and electrical energy requirements for aeration and cooling, and to compare batch and continuous modes of operation. Oxygen concentration in the liquid is a key input design variable, but its selection is challenging as it can result in design trade-offs. Reducing its value caused a decrease in electrical energy requirement, however this tended to increase the working volume of the bioreactor. The minimum or near-to-minimum total energy requirement for oxygen transfer occurred when operating at the onset of flooding throughout the bioreaction time. For typical KS values, continuous mode of operation required a much smaller bioreactor volume, due to higher operating cell concentration, and this is a major advantage of continuous over batch.
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Tsao, Jia-Huey, and Wen-Teng Wu. "Global control of a continuous stirred tank bioreactor." Chemical Engineering Journal and the Biochemical Engineering Journal 56, no. 1 (December 1994): B69—B74. http://dx.doi.org/10.1016/0923-0467(94)87034-9.

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Bušs, Armands, Normunds Jēkabsons, Artūrs Šuleiko, Dagnija Loča, and Juris Vanags. "VISUALIZATION APPROACHES FOR STIRRED TANK BIOREACTORS." ENVIRONMENT. TECHNOLOGIES. RESOURCES. Proceedings of the International Scientific and Practical Conference 3 (June 20, 2019): 18. http://dx.doi.org/10.17770/etr2019vol3.4077.

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Computational Fluid Dynamics (CFD) is the analysis of fluid behaviour employing numerical solution methods. Using CFD it is possible to analyse simple and complex fluid-gas, fluid-fluid or fluid-solid interactions. Fluid dynamics is described with laws of physics in the form of partial differential equations also known as Navier-Stokes equations. Sophisticated CFD solvers transform these laws into algebraic equations which are solved by numerical methods. In this paper Ansys CFX and Fluent analysis systems as research methods are used to visualize flow patterns in a stirred tank bioreactor. The results obtained are informative and can be used to improve the yield of biomass. CFD analysis can save time and aid fluid system designing process. This approach is cheaper and faster compared to conventional build-and-test process. However, it should be noted that CFD analysis results are as accurate as the level of skill possessed by a CFD engineer therefore there are still place for hands-on testing. Authors have developed a stirred tank model and visualized flow patterns. The research presents experimental computation methods and the model setup key parameters. The developed model allows to predict flow patterns inside stirred systems and evaluate efficiency of the mixing process by analysing parameters such as velocity field, turbulence eddy frequency, shear strain rate and power input.
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Murrell, J., S. Punreddy, A. Verma, K. Mann, D. Jing, D. Kehoe, N. Sunil, K. Niss, and M. Rook. "Monitoring MSCs during expansion in a stirred tank bioreactor." Cytotherapy 15, no. 4 (April 2013): S16. http://dx.doi.org/10.1016/j.jcyt.2013.01.057.

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Kuyukina, Maria S., Anastasiya V. Krivoruchko, and Irena B. Ivshina. "Advanced Bioreactor Treatments of Hydrocarbon-Containing Wastewater." Applied Sciences 10, no. 3 (January 24, 2020): 831. http://dx.doi.org/10.3390/app10030831.

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This review discusses bioreactor-based methods for industrial hydrocarbon-containing wastewater treatment using different (e.g., stirred-tank, membrane, packed-bed and fluidized-bed) constructions. Aerobic, anaerobic and hybrid bioreactors are becoming increasingly popular in the field of oily wastewater treatment, while high concentrations of petroleum hydrocarbons usually require physico-chemical pre-treatments. Most efficient bioreactor techniques employ immobilized cultures of hydrocarbon-oxidizing microorganisms, either defined consortia or mixed natural populations. Some advantages of fluidized-bed bioreactors over other types of reactors are shown, such as large biofilm–liquid interfacial area, high immobilized biomass concentration and improved mass transfer characteristics. Several limitations, including low nutrient content and the presence of heavy metals or toxicants, as well as fouling and contamination with nuisance microorganisms, can be overcome using effective inocula and advanced bioreactor designs. The examples of laboratory studies and few successful pilot/full-scale applications are given relating to the biotreatment of oilfield wastewater, fuel-contaminated water and refinery effluents.
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Aguilar-López, R., and I. Neria-González. "Controlling continuous bioreactor via nonlinear feedback: modelling and simulations approach." Bulletin of the Polish Academy of Sciences Technical Sciences 64, no. 1 (March 1, 2016): 235–41. http://dx.doi.org/10.1515/bpasts-2016-0025.

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Abstract The aim of this work is to present a class of nonlinear controller with an exponential-type feedback in order to regulate the sulfate mass concentration via the input flow in a continuous stirred tank bioreactor of an anaerobic sulfate-reducing process. The corresponding kinetic terms in the bioreactor’s modeling are modeled by unstructured modeling approach, which was experimentally corroborated. A sketch of proof of the closed-loop stability of the considered system is done under the framework of Lyapunov theory. Numerical experiments are conducted to show the performance of the proposed methodology in comparison with a well-tuned sigmoid controller.
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Pinheiro, I. O., M. B. De Souza, and C. E. Lopes. "The dynamic behaviour of aerated continuous flow stirred tank bioreactor." Mathematical and Computer Modelling 39, no. 4-5 (February 2004): 541–66. http://dx.doi.org/10.1016/s0895-7177(04)90525-0.

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Petrov, M. M. "Investigation of Power Characteristics of small Scale Stirred Tank Bioreactor." Biotechnology & Biotechnological Equipment 19, no. 1 (January 2005): 205–9. http://dx.doi.org/10.1080/13102818.2005.10817183.

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Dissertations / Theses on the topic "Stirred tank bioreactor"

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Di, Rado Federica. "analysis of stirred tank and orbitally shaken bioractors with non- newtonian fluid: an experimental study." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2021. http://amslaurea.unibo.it/22436/.

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Gas–liquid mixing equipment is applied widely in chemical industries. Many research available in literature have been carried out on this topic, but the majority of the studies deal with fluids of water-like viscosity. However, in practical industrial process, such as in pharmaceutical engineering, the process fluid exhibits non-Newtonian characteristics. The main objective of this study is to investigate the rheological properties influence of a non-Newtonian shear thinning fluid as representative of a fermentation broth. The study will involve two experimental setup, i.e. stirred tank bioreactor (STB) and orbitally shaken bioreactor (OSB), for comparison purposes. The hydrodynamic behavior of the non-newtonian fluid will be investigated in both bioreactors, with the aim to understand the main differences between such fluid and water. Furthermore, both systems will be analyzed under the process aspect, with particular attention to gas-to-liquid mass transfer. The goal of the project is to compare the widely-employed STB with the new OSB, considered more suitable for low shear stresses applications.
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Matongo, Tarisayi Martin. "Preliminary investigation of growth and antimicrobial production by streptomyces polyantibioticus : from shake flask to stirred tank bioreactor." Master's thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/20508.

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Resistance to antibiotics by microbial pathogens continues to be a major global health problem. Treatment of bacterial infections is becoming increasingly complex and expensive. Tuberculosis (TB), caused by Mycobacterium tuberculosis infection, is affected by antibiotic resistance. In South Africa, the Western Province is the worst affected, with an increasing incidence of both multi-drug resistant (MDR) and extensively drug resistant (XDR) strains of M. tuberculosis. Both resistant forms of TB increase the length of treatment to almost 24 months and cost by as much as 1400 times that of regular anti-tubercular chemotherapy. A potential solution to this problem is the discovery of new drugs, which can be obtained from natural sources. Actinomycetes are good sources of these drugs, with over 45% of current medicines derived from these bacteria. The actinobacterium Streptomyces polyantibioticus SPRT (SPRT) was locally isolated and first described by Le Roes (2006). It has been shown to produce bioactive molecules active against a range of bacteria, including compounds (drugs) that have anti-tubercular properties. One of the anti-tubercular molecules was identified as 2,5-diphenyloxazole (DPO). DPO is currently used as a component of scintillation fluid for its luminescent properties and is synthesised chemically in industry. SPRT is the only reported biological source of DPO, it is however not yet produced commercially via a biological route. The present study was performed to inform future process development of DPO production from SPRT. An investigation into the growth and production of antimicrobial compounds from submerged cultures of SPRT in shake flasks, and scale-up of the process into a laboratory stirred tank bioreactor (STR) was done in the present study. The work focused on obtaining growth kinetics and suitable operating conditions for cultivation. Characterisation of the growth profile of SPRT and determination of the kinetic growth parameters was carried out. Additionally, the antimicrobial production phases, and factors influencing their production was investigated. It was determined that the most reliable method of measuring biomass concentration was by dry cell gravimetric measurement of whole shake flasks following vacuum filtration, as it best suited the non-homogenous filamentous nature of SPRT.
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Hatton, Taylor Stephen. "Productivity Studies Utilizing Recombinant CHO Cells In Stirred-Tank Bioreactors: A Comparative Study Between The Pitch-Blade And The Packed-Bed Bioreactor Systems." DigitalCommons@USU, 2012. https://digitalcommons.usu.edu/etd/1267.

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A recombinat Chinese Hamster Ovary (rCHO) cell line designated as CHO SEAP was utilized in this investigation to optimize protein production. Two bench top stirred-tank bioreactors, namely a pitched-blade and a packed-bed basket bioreactor, were utilized for a comparative study to determine which bioreactor would produce the best results in terms of protein production. The objective of this research project was to provide basic data that shows cells cultured in a packed-bed basket bioreactor in perfusion mode will generate more protein product than cells in batch mode suspension culture with a pitched-blade bioreactor. The packed-bed bioreactor creates a homeostatic environment similar to the environment found in vivo, where waste products are constantly removed and fresh nutrients are replenished. Closed batch cultures do not provide a homeostatic environment. In batch culture systems, nutrients are depleted and waste products accumulate. The results from this experiment could help investigators involved in protein and/or vaccine production facilities select the appropriate bioreactor and mode of operation to optimize cell productivity for generation of a specific protein product. CHO cells have been used for the production of vaccines, recombinant therapeutic proteins, and monoclonal antibodies, and these cells are now the cell line of choice in the biopharmaceutical industry. Traditional vaccine production methods in egg embryos are slow and outdated, whereas roller bottle-based cell culture techniques are time consuming and have limited scalability. These limitations justify the need for development of stirred tank bioreactors. Cells cultured in a packed-bed bioreactor are not exposed to hydrodynamic forces, as is the case with pitched-blade bioreactors, allowing for maximum growth and protein expression. This mode of operation involves the constant removal of media depleted of nutrients and the addition of fresh media with more nutrients to keep the cells growing. Long run times decrease the constant need for re-seeding cells and re-establishing seed cultures, thus, reducing setup time and labor dramatically. Secreted products are automatically separated from cells in perfusion, eliminating filtration and membrane fouling. A detailed description of both modes of operation are discussed in this thesis.
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Loubière, Céline. "Characterization and impact of the hydrodynamics on the performance of umbilical-cord derived stem cells culture in stirred tank bioreactors." Thesis, Université de Lorraine, 2018. http://www.theses.fr/2018LORR0220/document.

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Les cellules souches mésenchymateuses (CSM) interviennent de plus en plus dans le domaine de la médecine régénérative, notamment pour traiter des maladies aujourd’hui difficilement curables avec les moyens actuels. Deux verrous scientifiques limitent pourtant leur utilisation et leur commercialisation. D’une part, de grandes quantités de cellules sont nécessaires pour répondre à la forte demande médicale. D’autre part, les cellules étant elles-mêmes le médicament final, délivré chez le patient, leur qualité doit être préservée (phénotype souche, capacité de différenciation). La mise en culture de ces cellules, sur des microporteurs, en bioréacteur agité, semble répondre à ces enjeux. Cependant, une connaissance plus précise de l’impact, sur la réponse physiologique des cellules, des technologies utilisées et de l’hydrodynamique générée est nécessaire pour améliorer les lois d’extrapolation des bioréacteurs de culture de CSM. Dans ce contexte, des travaux ont été mis en œuvre pour étudier l’influence du mode d’agitation (orbital ou mécanique) sur l’attachement, l’expansion et le détachement de CSM issues de la gelée de Wharton (GW-CSM) de cordons ombilicaux, sur des microporteurs de différentes compositions. Pour contribuer à la quantification de l’expansion cellulaire, une méthode de comptage automatique in situ a été développée pour estimer le nombre de cellules par microporteur, ainsi que leur répartition, sans avoir à procéder à leur détachement. Des microporteurs commerciaux ont ensuite pu être comparés à des microporteurs synthétisés dans un laboratoire partenaire, en termes d’attachement et expansion cellulaire, ainsi que de facilité de détachement. En parallèle de ces travaux, l’impact de la conception du mobile d’agitation, en bioréacteur mécaniquement agité, sur la mise en suspension de microporteurs a été analysé. A l’issue de cette étude, une analyse dimensionnelle et des simulations CFD ont été mises en place et deux modèles reliant la fréquence minimale de juste mise en suspension (Njs) avec la géométrie du mobile d’agitation (forme, taille, position dans la cuve) et les propriétés matérielles des particules et de la phase liquide ont été proposés. Une stratégie d’optimisation des paramètres géométriques d’un mobile en minibioréacteur, dédié à la culture de CSM sur microporteurs, a été mise en place, à partir de paramètres caractérisant les contraintes hydromécaniques perçues par la phase solide, judicieusement choisis et intégrés lors des simulations CFD. Selon un plan d’expérience, et les résultats extraits des simulations, des surfaces de réponse ont été construites et une optimisation multi-objective a été réalisée afin de déterminer la géométrie minimisant les contraintes perçues par les particules, et donc par les cellules adhérées. Des cultures de GW-CSM en minibioréacteurs équipés de différents mobiles ont finalement été validées, avec une comparaison préliminaire de l’impact de ces géométries sur l’expansion cellulaire
Mesenchymal stem cells (MSC) are becoming increasingly involved in the regenerative medicine field, particularly to treat diseases that are not effectively curable with the current therapies. Two scientific barriers are nevertheless responsible for MSC use and commercialization limitations. On one side, large amounts of cells are needed to reach the high cell dose requirements. On the other side, cells being the final product themselves, directly injected into the patient, their quality have to be controlled (stem cell phenotype, differentiation capability). MSC cultivation on microcarriers in a stirred bioreactor seems to meet these challenges. However, a precise knowledge about the impact of the technologies and the hydrodynamics generated, on the physiological cell response, is necessary to improve the scale-up of MSC cultures in bioreactors. In this context, present work is dedicated to the study of the impact of the agitation mode (orbital or mechanical) on the cell attachment, expansion and detachment on various microcarrier types, in the case of MSC derived from the Wharton’s jelly (WJ-MSC) of umbilical cords. To quantify more precisely cell distribution and expansion on microcarriers, an automatic and in situ counting method was developed, which need no detachment step. This allowed the identification of commercial microcarriers suitable for WJ-MSC cultures, which were then compared to home-made microcarriers, synthesized by a partner laboratory, in terms of cell attachment and expansion, and detachment efficiency. In parallel to these works, the impact of the impeller design on the microcarrier suspension in stirred tank bioreactors was investigated. Based on a dimensional analysis and CFD simulations, it resulted in the establishment of two models relating the minimal agitation rate to ensure all particle suspension (Njs) with the impeller geometrical characteristics (design, size, off-bottom clearance) and the material properties of both the solid and the liquid phases. CFD models validation allowed then to develop a strategy to optimize the geometrical configuration of an impeller, dedicated to MSC cultures on microcarriers in a minibioreactor. Parameters characterizing the hydromechanical stress encountered by the solid phase were wisely chosen and integrated into CFD simulations. Based on a design of experiments, and the hydrodynamics data recovered from simulations, response surfaces were built and a multiobjective optimization was achieved in order to determine the geometry minimizing the particle stress, and also by adhered cells. WJ-MSC cultures in minibioreactors equipped with impellers displaying various geometries were finally validated, with a preliminary comparison of the impact of these geometries on the cell expansion
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Rafiq, Qasim Ali. "Developing a standardised manufacturing process for the clinical-scale production of human mesenchymal stem cells." Thesis, Loughborough University, 2013. https://dspace.lboro.ac.uk/2134/12335.

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Human mesenchymal stem cells (hMSCs) are a promising candidate for cell-based therapies given their therapeutic potential and propensity to grow in vitro. However, to generate the cell numbers required for such applications, robust, reproducible and scalable manufacturing methods need to be developed. To address this challenge, the expansion of hMSCs in a microcarrier-based bioreactor system was investigated. Initial studies performed in T-flask monolayer cultures investigated the effect of key bioprocess parameters such as dissolved oxygen concentration (dO2), the level of medium exchange and the use of serum-free media. 20 % dO2 adversely impacted cell proliferation in comparison to 100 % dO2, whilst FBS-supplemented DMEM was found to be the most consistent and cost-effective cell culture medium despite the advances in serum-free cell culture media. Several microcarriers were screened in 100 mL agitated spinner flasks where Plastic P102-L was selected as the optimal microcarrier for hMSC expansion given the high cell yields obtained, its xeno-free composition and effective harvest capacity. The findings from the initial small-scale studies culminated in the successful expansion of hMSCs on Plastic P102-L microcarriers in a fully equipped 5 L stirred-tank bioreactor (2.5 L working volume), the largest reported volume for hMSC microcarrier culture to date. A maximum cell density of 1.68 x 105 cells/mL was obtained after 9 days in culture; further growth was limited by the low glucose concentration and lack of available surface area. A novel, scalable harvesting method was also developed, allowing for the successful recovery of hMSCs. Importantly, harvested hMSCs retained their immunophenotype, multipotency and ability to proliferate on tissue culture plastic.
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Jambi, Ebtihaj J. "Comparative studies on the cultivation of Xanthomonas campestris in submerged culture for the production of xanthan using the traditional industrial stirred tank reactor and a novel oscillatory baffled bioreactor." Thesis, University of Strathclyde, 2012. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=18713.

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Xanthan is a well-known extracellular polysaccharide, produced by a Gram negative bacterium Xanthomonas campestris (X. campestris) under aerobic conditions. Solutions of xanthan exhibit high viscosities and non-Newtonian behaviour even at low concentrations. This biopolymer has a wide range of valuable commercial and industrial applications, for example; it can be used as a food thickening agent and a stabilizer in some other industries. Traditionally the production of xanthan has predominantly been performed in stirred tank fermenter (STR). This study sought to compare the cultivation of the bacterium, X. campestris for the production of the viscous biopolymer xanthan gum in two different reactor systems, a novel oscillatory baffled reactor (OBR) and the conventional industry workhorse, the stirred tank reactor (STR). Overall biopolymer production occurred at similar rates in the well stirred and aerated STRs, albeit at the cost of higher energy inputs for mixing and aeration. Despite much previous literature promoting the use of the OBR for transporting and reacting very viscous systems, this was the first actual study attempting to investigate the use of the OBR for a highly viscous non-Newtonian fermentation process. The experimental results show that xanthan production was similar in the OBR than in the STR, the OBR is however readily suitable for the cultivation of xanthan. The probable reasons for the inability of the OBR to match the production rates of the STR may well lie in the complex nature of this fermentation process. Unlike a previous study on pullulan production (Gaidhani 2004) where the OBR outperformed the STR, X. campestris initially needs high oxygen transfer rates and the OBR, although it provides good bulk mixing and low energy consumption, seemed unable to equal the STR in this respect, especially in a very viscous system. The result shows that xanthan production in the OBR was similar to the equivalent process in the STR. In order to attempt to improve the OBR a number of technical modifications were made including a novel sparger design to improve gas dispersal. These were not successful in improving xanthan production. Similarly, attempts to achieve improvements via wider amplitude ranges led to damage to the equipment. The conclusion was that significant improvements to the physical robustness of the OBR were necessary before it could be successfully used to process highly viscous bio-fluids.
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Olughu, Williams C. "The systematic consideration of the large-scale fed-batch fermentation inhomogeneities using a genetically modified C. glutamicum strain as a model organism." Thesis, Loughborough University, 2018. https://dspace.lboro.ac.uk/2134/34284.

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The loss of efficiency and performance of bioprocesses on scale-up is well known, but not fully understood. This work addresses this problem, by studying the effect of some fermentation gradients (pH, glucose and oxygen) at a larger scale in a bench-scale two compartment reactor (PFR + STR) using the cadaverine-producing recombinant bacterium, Corynebacterium glutamicum DM1945 Δact3 Ptuf-ldcC_OPT. The initial scale down strategy increased the magnitude of these gradients by only increasing the mean cell residence time in the plug flow reactor (τ_PFR). The cell growth and product related rate constants were compared as the τ_PFR was increased; differences were significant in some cases, but only up to 2 min residence time. For example, losses in cadaverine productivity when compared to the control fed-batch fermentation on average for the τ_PFR of 1 min, 2 min and 5 min were 25 %, 42 % and 46 % respectively. This indicated that the increasing the τ_PFR alone does not necessarily increase the magnitude of fermentation gradients. The new scale-down strategy developed here, increased the magnitude of fermentation gradients by not only increasing the τ_PFR, but also considering the mean frequency at which the bacterial cells entered the PFR section (f_m). The f_m was kept constant by reducing the broth volume in the STR. Hence, the bacterial cells also spent shorter times in the well mixed STR, as the τ_PFR was increased (hypothesised as giving the bacterial cells less time to recover the non-ideal PFR section of the SDR). On adoption of this strategy cadaverine productivity decreases for the τ_PFR of 1 min, 2 min and 5 min were 25 %, 32 % and 53 % respectively. Thus, highlighting that loss in performance is most likely to occur as the magnitude of heterogeneity within the fermentation environment increases. However, Corynebacterium glutamicum DM1945 Δact3 Ptuf-ldcC_OPT did show some resilience in its biomass productivity. It was only marginally affected in the harshest of conditions simulated here.
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Regestein, Lars [Verfasser]. "Design and application of calorimeters for monitoring biological processes in stirred tank bioreactors / Lars Regestein." Aachen : Hochschulbibliothek der Rheinisch-Westfälischen Technischen Hochschule Aachen, 2013. http://d-nb.info/1031109536/34.

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Lutsinge, Tshilidzi Bridget. "Biosurfactant enhanced biodegradation of high molecular weight polycyclic aromatic hydrocarbons in a two-stage continuous stirred tank bioreactors and biofilm tank." Diss., University of Pretoria, 2017. http://hdl.handle.net/2263/66214.

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Polycyclic aromatic hydrocarbons (PAHs) do not dissolve easily in water, due to their hydrophobic properties. PAHs are unavailable to most aromatic compound degrading organisms, due to these properties. In this study, a biosurfactant producing culture enhancing dissolution of PAHs was isolated, to make them bioavailable. The culture was introduced to the system to improve the dissolution of PAHs and degrade the PAHs thereafter. The aim of the study was to use a strategy with a biofilm process, subsequent to a continuous stirred tank bio-reactors (CSTRs) to successfully remove PAHs from water, with microorganisms that can degrade these pollutants. The open system could easily be controlled and set to optimum conditions, stimulating the growth of PAH degraders. The feed rate and influent concentration can be controlled and the system can easily be cleaned. Biodegradation was achieved, using optimum conditions obtained from the conducted batch studies in a CSTR process ensuring a feasible biodegradation process. Two cultures, Pseudomonas aeruginosa and microbial consortia, were used during the biosurfactant production and PAHs degradation preliminary batch studies. The biosurfactants produced, were identified as Lipopeptides and degradation results indicated great degradation of fluoranthene and triphenylene with a mixed culture consortium present in the system. 90.1% of fluoranthene and 79.6% of triphenylene was degraded after 22 d of incubation in the batch system. Degradation of fluoranthene was studied using biosurfactants and microbial consortium in a three-stage continuous flow system. Reactor 2A fluoranthene influent (60.89%) was degraded, 70.02% of Reactor 2B fluoranthene influent was degraded and 77.17% of biofilm tank fluoranthene influent was degraded. Kinetic studies were conducted, using a Monod model to describe the substrates degradation for batch systems. The highest degradation rate for fluoranthene was determined to be 0.29 h-1 and for triphenylene was 0.13 h-1 with half saturation values of 991.84 mg/L and 451 mg/L respectively, indicating that fluoranthene was degraded faster than triphenylene, when incubated for 22 d. The study demonstrated that biosurfactant production and biodegradation of fluoranthene can be achieved in an open CSTR system, as much as it can be done in a batch system. The biological remediation of PAHs in wastewater plants can be introduced and applied for wastewaters rich, with PAHs.
Dissertation (MSc)--University of Pretoria, 2017.
Chemical Engineering
MSc
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Amoabediny, Ghassem. "A new method to quantify the CO2 sensitivity of micro-organisms in shaken bioreactors and scale up to stirred tank fermentors." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=982158823.

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Books on the topic "Stirred tank bioreactor"

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Chamsāt, Sētthawat. Rāingān kānwičhai rư̄ang kānʻō̜kbǣp phatthanā læ kānkhayāi sūan patikō̜n chīwaphāp bǣp thangkūan samrap kānsalāi pǣng mansampalang =: Design, development, and scale-up of stirred tank lysis bioreactor for enzymatic hydrolysis of cassava starch. [Chonburi]: Khana Witthayāsāt, Mahāwitthayālai Būraphā, 2006.

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Henderson, Kelley. Oxygen mass transfer and shear sensitivity studies during cultivation of Nicotiana tabacum var. Wisconsin 38 in a stirred-tank bioreactor. 1991.

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Ramanan, Sundar. Biomass productivity enhancement of Laminaria saccharina cultures in a stirred-tank bioreactor by batch and fed-batch nutrient delivery. 1996.

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Ho, Chung-Han. Shear sensitivity and oxygen mass transfer studies during cultivation of tobacco cells in a stirred-tank bioreactor of impeller speeds of 100 to 325 rpm. 1994.

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Rāingān kānwičhai rư̄ang kānʻō̜kbǣp phatthanā læ kānkhayāi sūan patikō̜n chīwaphāp bǣp thangkūan samrap kānsalāi pǣng mansampalang =: Design, development, and scale-up of stirred tank lysis bioreactor for enzymatic hydrolysis of cassava starch. [Chonburi]: Khana Witthayāsāt, Mahāwitthayālai Būraphā, 2006.

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Book chapters on the topic "Stirred tank bioreactor"

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Monteil, Dominique T., and Jeffrey Kuan. "Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension." In Methods in Molecular Biology, 133–45. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8730-6_10.

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Kallel, H., S. Rourou, A. Van Der Ark, M. Thalen, and T. Van Der Velden De Groot. "Design of an Animal Protein Free Medium to Sustain Vero Cells Growth in a Stirred Tank Bioreactor." In Animal Cell Technology Meets Genomics, 775–78. Dordrecht: Springer Netherlands, 2005. http://dx.doi.org/10.1007/1-4020-3103-3_161.

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Reuss, Matthias, Sven Schmalzriedt, and Marc Jenne. "Application of Computational Fluiddynamics (CFD) to Modeling Stirred Tank Bioreactors." In Bioreaction Engineering, 207–46. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-59735-0_8.

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Manstein, Felix, Caroline Halloin, and Robert Zweigerdt. "Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors." In Methods in Molecular Biology, 79–91. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9477-9_7.

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Wang, Huaqing, Daniel Kehoe, Julie Murrell, and Donghui Jing. "Structured Methodology for Process Development in Scalable Stirred Tank Bioreactors Platforms." In Bioprocessing for Cell Based Therapies, 35–64. Chichester, UK: John Wiley & Sons, Ltd, 2016. http://dx.doi.org/10.1002/9781118743362.ch2.

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Simão, Daniel, Francisca Arez, Ana P. Terasso, Catarina Pinto, Marcos F. Q. Sousa, Catarina Brito, and Paula M. Alves. "Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells." In Methods in Molecular Biology, 129–42. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/7651_2016_333.

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"Stirred Tank Bioreactors." In Bioreactor System Design, 227–76. CRC Press, 1994. http://dx.doi.org/10.1201/9781482277470-10.

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"Stirred-Tank Bioreactors." In An Introduction to Bioreactor Hydrodynamics and Gas-Liquid Mass Transfer, 69–123. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2014. http://dx.doi.org/10.1002/9781118869703.ch6.

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Garcia-Ochoa, F., V. E. Santos, and E. Gomez. "Stirred Tank Bioreactors." In Comprehensive Biotechnology, 179–98. Elsevier, 2011. http://dx.doi.org/10.1016/b978-0-08-088504-9.00108-2.

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Garcia-Ochoa, F., V. E. Santos, and E. Gomez. "Stirred Tank Bioreactors." In Comprehensive Biotechnology, 270–90. Elsevier, 2019. http://dx.doi.org/10.1016/b978-0-444-64046-8.00078-1.

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Conference papers on the topic "Stirred tank bioreactor"

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Marin, Constantin, Dan Selisteanu, Dan Popescu, and Monica Roman. "Adaptive optimal control of a Continuous Stirred Tank Bioreactor." In 2015 19th International Conference on System Theory, Control and Computing (ICSTCC). IEEE, 2015. http://dx.doi.org/10.1109/icstcc.2015.7321268.

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Kadic, Enes, and Theodore J. Heindel. "Hydrodynamic Considerations in Bioreactor Selection and Design." In ASME 2010 3rd Joint US-European Fluids Engineering Summer Meeting collocated with 8th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2010. http://dx.doi.org/10.1115/fedsm-icnmm2010-30367.

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The biological production of renewable fuels and chemicals, medicines, and proteins is not possible without a properly functioning bioreactor. Bioreactors are expected to meet several basic requirements and create conditions favorable to the biological material such that the desired production is maximized. The basic requirements, which are strongly influenced by fluid mechanic principles, may include minimum damage to the biological material, maximum reactor volume utilization, optimized gas-liquid mass transfer, and/or enhanced mass transfer from the liquid to the biological species. Each of these goals may be achieved within any of the major bioreactor designs, which generally fall under the categories of stirred tank, bubble column, or airlift bioreactor. Yet, each of the bioreactor designs has strengths and weaknesses. This paper provides an overview of bioreactor hydrodynamic developments and the fluid mechanic issues that should to be considered when selecting a bioreactor for experimental and production purposes.
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Gelves, R. "CFD simulation of liquid-liquid dispersions in a stirred tank bioreactor." In 11TH INTERNATIONAL CONFERENCE OF NUMERICAL ANALYSIS AND APPLIED MATHEMATICS 2013: ICNAAM 2013. AIP, 2013. http://dx.doi.org/10.1063/1.4825982.

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Nwaigwe, Kevin N., Nnamdi V. Ogueke, Chibuike Ononogbo, and Emmanuel E. Anyanwu. "Performance Study of Anaerobic Digestion of Organic Municipal Waste in Upflow Bioreactor With Central Substrate Dispenser." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-64064.

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A performance study of anaerobic digestion of organic municipal waste in upflow bioreactor with central substrate dispenser is presented. The experimental rig is based on an integrated system of bioreactors consisting of Upflow Bioreactor (UB), Upflow Bioreactor with Central Subtrate Dispenser (UBCSD), and Continous Stirred Tank Reactor (CSTR) each having internal volume of 76 litres, 64.8 litres, and 76 litres respectively. The scheme is used for minimizing the mixing and fouling problems associated with some conventional bioreactors during digestion reaction. Organic municipal waste (OMW) was used to prepare the slurry for the reactors. Microbial reaction was enhanced during operation using a measured quantity (2kg) of substances from the rumen of a newly slaughtered cow. The experimentation from feeder tank to Bioreactors was carried out for a period of 10-days Hydraulic Retention Time (HRT) at 37°C. Effects of some basic parameters affecting anaerobic digestion in terms of biogas production and Chemical Oxygen Demand (COD) reduction were carried out. They include substrate temperature, minimal average temperature, changes in temperature, substrate content and properties, available nutrient, retention time, organic loading rate, pH level, nitrogen inhibition and C/N ratio, substrate agitation, and inhibitory factors. Results showed that UBSCD generated the highest level of Biogas yield of up to 52915 ml, while UB and CSTR yielded 23550ml and 28980ml respectively. Similarly for COD removal, 24343 mg/l, 5775.4 mg/l, and 23155 mg/l were achieved for UBCSD, UB and CSTR respectively from an initial value of 120,320 mg/l. These results show that the use of UBCSD better enhances biofuel production from organic municipal waste.
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Daaou, Bachir, and Denis Dochain. "High order sliding mode observer based extremum seeking controller for a continuous stirred tank bioreactor." In 2015 3rd International Conference on Control, Engineering & Information Technology (CEIT). IEEE, 2015. http://dx.doi.org/10.1109/ceit.2015.7233018.

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Liovic, Petar, Ilija D. Šutalo, Laurence Meagher, and George O. Lovrecz. "Computations of Flow Environments in Medium-Scale Stirred-Tank Bioreactors for Stem Cell Expansion." In ASME 2014 4th Joint US-European Fluids Engineering Division Summer Meeting collocated with the ASME 2014 12th International Conference on Nanochannels, Microchannels, and Minichannels. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/fedsm2014-21967.

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The flow in a New Brunswick Scientific (NBS (now Eppendorf)) 5 L stirred-tank bioreactor (STR) partially filled with 2.2 L of water and agitated at 60 rpm using a pitched-blade impeller is studied in this work, to determine the suitability of the configuration for expanding stem cell lines. Computational Fluid Dynamics (CFD) model development and testing in this work has found Large Eddy Simulation (LES) to be essential for model fidelity and for capturing spatiotemporal stress fluctuations. Stresses were at levels similar to or even higher than those known to damage stem cells or modulate their cellular function to favour differentiation instead of phenotype maintenance. The results raise questions as to the appropriateness of such STRs for stem cell expansion, and motivate better experimental studies to properly quantify the spatiotemporal variability in fluid shear stresses and its effect on stem cell expansion and stem cell fate.
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Kapadia, A., N. Nath, T. C. Burg, and D. M. Dawson. "Lyapunov-based continuous-stirred tank bioreactor control to maximize biomass production using the haldane and monod specific growth models." In 2010 American Control Conference (ACC 2010). IEEE, 2010. http://dx.doi.org/10.1109/acc.2010.5531387.

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Kadic, Enes, and Theodore J. Heindel. "Mixing Considerations in Stirred Tank Bioreactors When Using Fluid Property Altering Microorganisms." In ASME 2010 3rd Joint US-European Fluids Engineering Summer Meeting collocated with 8th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2010. http://dx.doi.org/10.1115/fedsm-icnmm2010-30366.

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Stirred tank reactors are one of the standard reactors in the chemical industry and have been widely implemented for biological applications. They are used with viscous liquids, slurries, very low gas flow rates, and large liquid volumes. Stirred tank bioreactors are popular because a well-mixed state, required or preferred for numerous biological processes, is usually achieved in such situations; however, many production processes using microorganisms tend to experience fluid property alterations, which significantly impact mixing, operational parameters, and process results. The most troubling issues occur when a fluid gradually undergoes a viscosity change and/or slowly exhibits non-Newtonian behavior due to microorganism growth since these will alter the flow conditions and possibly limit the conversion rate or production scale. This paper provides an overview of the relevant mixing issues in stirred tank bioreactors when using a range of fluid viscosities, surface tensions, and/or non-Newtonian fluids.
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