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1
Vingytė, Aistė. "Šunų ir kačių stomatitų, gingivitų, periodontitų susirgimų analizė Kauno miesto "X" ir "Y" veterinarijos klinikose 2011 - 2013 metais." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_134434-16519.
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Darbo pavadinimas: Šunų ir kačių stomatitų, gingivitų ir periodontitų susirgimų analizė Kauno miesto “X” ir “Y” veterinarijos klinikose 2011 – 2013 metais.
Lietuvos Sveikatos Mokslų Universitetas, Veterinarijos akademija, Veterinarijos fakultetas.
Darbo apimtis 41 lapas.
Darbe yra 22 paveikslai.
Naudotų literatūros šaltinių kiekis: 34.
Darbe buvo ištirti šunys ir katės, sergantys stomatitu, gingivitu ir periodontitu. Pasirinktos „X“ ir „Y“ klinikos Kaune. Darbas buvo atlikas 2011.11 – 2013.11 laikotarpiu. 2011.11 – 2013.11 laikotarpiu „X“ ir „Y“ klinikose iš viso buvo gydyti 2455 pacientai. Šiais tirtais metais burnos ertmės susirgimai buvo diagnozuoti 304 pacientams, iš jų 200 buvo šunys ir 104 – katės.
Darbo tikslas: išanalizuoti ir įvertinti šunų ir kačių burnos ertmės ligų pasireiškimo dažnumą ir įtakos turinčius faktorius.
Darbo uždaviniai: išanalizuoti ir įvertinti burnos ertmės ligų pasireiškimo dažnumą ir gausumą, jų priklausomybę nuo amžiaus, lyties ir veislių dydžio.
Remiantis tyrimų duomenimis, nustatyta, kad „X“ ir „Y“ klinikose buvo gydyti 304 pacientai (12,0 proc.), kurie sirgo stomatitais, gingivitais ir periodontitais. Iš jų 200 (8,0 proc.) pacientų buvo šunys ir 104 (4 proc.) pacientai buvo katės. Dažniausiai šunims diagnozuotas burnos ertmės susirgimas buvo periodontitas – 56 (43,0 proc.) pacientai 1-5 metų amžiaus grupėje, dažniausiai katėms diagnozuotas burnos ertmės susirgimas buvo gingivitas – 20 (43,47 proc.) pacientų 1-5 metų amžiaus... [toliau žr. visą tekstą]Topic title: Analysis of dogs and cats stomatitis, gingivitis and periodontitis diseases in Kaunas “X” and “Y” veterinary clinics 2011 – 2013. Education institution: Lithuanian University of Health Sciences, Veterinary Academy, Veterinary faculty. The ambit of the work is 41 pages. In the final work the number of pictures are: 22 pictures. A list of used literature: 34 references. In this work has been investigated the dogs and cats with stomatitis, gingivitis, periodontitis. Selected the „X“ and „Y“ clinics in Kaunas. Work was done by 2011.11 - 2013.11 years period. 2011.11 - 2013.11 in „X“ and „Y“ clinics were treated 2455 patients. During this period, 304 patients were diagnosed with oral diseases, of which 200 were dogs and 104 - cats. The aim: analyze and evaluate the frequency of dogs and cats oral cavity diseases and their influencing factors. Tasks of work. To evaluate the frequency and abundance of oral cavity diseases, dependence on age, sex and breed size. Based on the research, it was found that, in „X“ and „Y“ clinics were treated 304 patients (12,0 %), who had stomatitis, gingivitis, periodontitis. 200 (8,0 %) of them were dogs and 104 (4,0 %) were cats. The most common oral disease in dogs has been periodontitis - 56 (43 %) patients in the age group of 1-5 years and cats the most common oral disease was gingivitis - 20 (43,47 %) patients in the age group of 1-5 years. Dependence oral cavity diseases on sex has not been determined. It was found that... [to full text]
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Paškevič, Dorota. "Kačių dantų ligos. Jų gydymas, profilaktika." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_135159-43398.
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Veterinarinė odontologija – tai nauja greitai besivystanti veterinarinės medicinos sritis. Daugelyje šalių veterinarinė odontologija jau egzistuoja kaip atskira disciplina, tirianti dantų ir burnos ertmės ligas, profilaktiką bei gydymą.
Remiantis statistiniais duomenimis, dantų, žandikaulio ir burnos ertmės ligos gyvūnų tarpe - labai paplitusios visame pasaulyje. Daugiau nei 95 proc. kačių ir šunų per visą jų gyvenimą pasireiškia dantų ir burnos ertmės patologijos. Kadangi kačių dantų ligų gydymas yra gana sudėtingas ir skausmingas gyvūnui procesas, geriau tinka taikyti profilaktikos priemones, nei gydymą. Todėl nuo mažens gyvūną reikia pratinti prie burnos ertmės higienos.
Lietuvoje dar labai mažai dėmesio skiriama šiai problemai. Deja, tik 8 proc. gyvūnų savininkų laiku pastebi dantų ligų požymius. Šeimininkai neskiria reikalingo dėmesio savo augintinio burnos ertmės priežiūrai, nesusimąstydami, kad dantų ligos gali būti bendros gyvūno savijautos pablogėjimo ir daugelio ligų priežastimi.
Darbo tikslas – nustatyti kačių sergamumą dantų ligomis, bei apžvelgti jų gydymą ir profilaktikos priemonių taikymą.
Darbo uždaviniai:
1. išanalizuoti ir apžvelgti literatūros šaltinius, susijusius su šia tema;
2. išnagrinėti dantų ligų diagnozavimo ir gydymo metodus;
3. išsiaiškinti ar kačių lytis turi įtakos dantų ligų pasireiškimui;
4. nustatyti kokiomis dantų ligomis dažniausiai serga katės;
5. nustatyti kokios ligos kokiose amžiaus grupėse paplitusios dažniausiai;
6. nustatyti... [toliau žr. visą tekstą]The aim of this work is to determine morbidity of feline dental disease,and review the treatment and prevention measures. The objectives: 1. To examine and review literature sources and information concerning the work objectives. 2. To review dental disease diagnostic and treatment methods. 3. To detemine whether cat gender has an effect on dental diseases. 4. To determine which dental diseases are the most popular in cats. 5. To determine what kind of dental diseases are the most popular in age groups. 6. To determine which breed of cats most likely have dental diseases. 7. To analyze prevention measures of dental disease. The research results and conclusions are: Cat sex does not have an effect on dental diseases. Dental diseases are most likely to develop in mixed breed cats. The most popular dental disease in cats is calculus. Mostly dental diseases affect cats which are older than 10 years. Periodontitis usually affects cats at age of 7 to 9 and those older than 10 years. Dental diseases mostly occur in the Siamese – oriental group of cat, and less longhaired cat group. The most common prevention measure is the specific cat food for teeth. Most cat owners do not clean their cat teeth in veterinary clinic. In conclusion, the cat owners do not have enough knowledge about dental diseases, its treatment and prevention factors.
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Bissell, Vincent. "Denture stomatitis : clinical and laboratory studies." Thesis, University of Edinburgh, 1995. http://hdl.handle.net/1842/21623.
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This thesis describes investigations conducted to study factors influencing the outcome of antifungal treatment of denture stomatitis. Clinical, mycolognic, haematologic and prosthodontic factors were evaluated. The humoral and cellular immune responses were also studied. Two antifungal agents were compared: systematically administered fluconazole and topically administered amphotericin. A total of 29 patients were randomised to receive fluconazole 50mg daily for 14 days; 30 were randomised to receive amphotericin lozenges and cream for 28 days. Patients were assessed at the time of entry into the study and at one, four and twelve weeks thereafter. There were no significant differences in clinical response between the groups at any follow-up visit. The best clinical response was observed after four weeks whereas the best mycologic response was noted after one week. At the 12 week assessment clinical evidence of relapse and recurrence was a common finding irrespective of treatment. Overall the correlation between mycologic and clinical events was poor. Correlation was better in those patients who demonstrated very poor and very good clinical responses. Fluconazole appeared to be largely ineffective against Candida glabrata. Haematologic deficiencies were infrequent and appeared not to affect clinical outcome. Clinical response in non-smokers was better than in smokers and this difference was significant at the one week visit. Denture quality although generally poor appeared to exert no marked influence over clinical outcome. Adverse reactions to treatment were uncommon in both groups. Little change was observed in serum and salivary total and anti-candidal antibodies throughout the study. However the humoral immune response in smokers appeared to be depressed in comparison with non-smokers.
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Durand, Emmanuel. "Stomatite et traitements." Paris 5, 1996. http://www.theses.fr/1996PA05P142.
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Presloid, John B. "Characterization of Vesicular Stomatitis Virus Strains with Adaptability." University of Toledo Health Science Campus / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=mco1225313889.
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Soh, Timothy Kinshiong. "Single particle studies of vesicular stomatitis virus assembly." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:17464089.
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The formation of viral particles requires the coordinated assembly of both nucleic acids and proteins. In the case of Rhabdoviruses, such as vesicular stomatitis virus (VSV), the particles display a characteristic bullet-shape. VSV virions consist of the matrix protein (M), glycoprotein (G), and viral ribonucleoprotein (RNP), which contains the nucleocapsid protein (N) coated RNA bound to the large polymerase protein (L) through the phosphoprotein (P). During assembly, these components are recruited to the plasma membrane where the viral RNP undergoes condensation by M and envelopment with G containing membranes. To address whether formation of the bullet-shape requires a consistent packaging of the viral proteins, the composition of single virions was measured with fluorescence microscopy. We generated autonomously replicating VSV bearing up to 3 fluorescent protein fusions in the disordered N-terminal region of M and N-terminus of P and G. Quantification of single particles reveals that VSV assembles with a range of M, P, and G molecules, suggesting a flexible packaging mechanism. The maintenance of the bullet-shape with significantly less M proposes that condensation does not require the particle to be saturated with M. Our fluorescent VSV clones permit the tracking of viral components in live cells. We observed that assembly of M into particles requires ~2 min and can be broken into 4 stages. First, M forms a small preassembly complex. Second, M rapidly assembles into particles where its incorporation initiates before P, although they are packaged concurrently. This is followed by a delay before final release of particles into the supernatant. Late domains in M were thought to only recruit the endosomal sorting complexes required for transport (ESCRT) pathway to mediate fission. However, using our M fusions we demonstrate that these motifs are required for efficient competition into released particles and a step in assembly prior to pinching off. These constructs have permitted the study of viral assembly at the single particle level and are useful tools for studying viral entry and egress. Specifically, VSV containing M-eGFP and the lassa virus glycoprotein instead of G was used to demonstrate the requirement of a host factor for lassa virus fusion.Medical Sciences
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Ghodratnama, Fatemeh. "Clinical and molecular biological studies in recurrent Aphthous Stomatitis." Thesis, University of Glasgow, 1997. http://theses.gla.ac.uk/2614/.
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The aim of these studies was to research different aspects of the pathogenesis and therapeutic features of recurrent aphthous stomatitis. In support of the involvement of viruses in the aetiology of recurrent aphthous stomatitis, the nested PCR and assays of ELISA and IFA were employed. Results of PCR investigations showed that HHV-6 DNA was present in 29 per cent of aphthous lesions. Using ELISA, specific IgG antibodies against HHV-6 were detected in 96.7 per cent of all serum samples with no significant difference between aphthous patients, oral lichen planus or control subjects. Specific IgM antibodies against HHV-6 were found in a higher prevalence rate in aphthous samples compared with the two other groups: a significant difference of p=0.01 was found between sera of aphthous patients compared with healthy controls. HCMV and VZV DNA were not detected in aphthous samples. Also serological findings did not show any significant increase in the prevalence of specific IgG antibodies against these two viruses. Serum IgM antibodies against HCMV were positive in a small number of samples with no difference between groups and IgM antibody against VZV was not positive in any serum samples. These data fail to show that recurrent aphthous stomatitis can be a manifestation of VZV or HCMV infection or reactivation. However, the possibility of involvement of HHV-6 is raised by the present studies. The possible involvement of Mycobacterium paratuberculosis was examined by the nested PCR investigations. Although mycobacterial DNA was detected only in four biopsy samples of aphthous patients and in none of the oral lichen planus patients or controls, this difference was not significant and more research is necessary to confirm such involvement.
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Cross, Laura Jayne. "Triazoles in the management of Candida-associated denture stomatitis." Thesis, University of Glasgow, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.264801.
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Hodges, Erin Nicole. "Characterization of the S2 isolate of vesicular stomatitis virus." Thesis, Boston University, 2013. https://hdl.handle.net/2144/12781.
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Thesis (Ph.D.)--Boston University
PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.Vesicular stomatitis virus is a prototype nonsegmented, negative-sense RNA virus that has been widely used to study common aspects of RNA virus replication. Attenuated mutants of VSV have led to important discoveries about viral function, particularly genome replication and mRNA transcription. S2 VSV is small plaque isolate of VSV initially described to be attenuated in vivo but still able to induce a robust interferon response. Detailed characterization of the attenuated VSV isolate S2 has verified that this isolate is attenuated and is able to induce a blocking antibody response in vivo. Further characterization showed that this isolate is antigenically and phylogenetically distinct from related wild-type VSV isolates. Sequencing of the virus shows that there are more than three hundred nucleotide changes from the standard VSV laboratory strain, San Juan. Characterization of the RNA products produced in S2 VSV infected cells has led to the discovery of a non-interfering subgenomic particle that is carried along with S2 VSV infection. Additionally, characterization of the attenuated phenotype showed that S2 VSV has markedly different transcription gradient when compared with San Juan VSV. S2 shows a steeper gradient of polymerase transcription than wild-type virus and a decline in total transcription after 4 hpi. As expected, this decline in active transcription leads to lower level of mRNA accumulation in S2 VSV infected cells. In a coinfection with wild-type VSV, the S2 pattern of transcription is completely dominant at all times in infection, and this altered transcription phenotype of S2 is not due to an innate cellular response, as transcription in vitro duplicates the phenotype seen in cells. S2 VSV is the first demonstrated viral mutant with a steeper gradient of transcription that is not dependent on RNA template sequence or host response. The attenuation and ability of S2 VSV to inhibit wild-type virus suggests that S2 VSV would be a good candidate vector for VSV-based vaccines. In addition, the mechanism behind the altered transcriptional profile of S2 VSV has led to new understanding of the role of the polymerase complex in the unique mechanism of transcriptional control by the nonsegmented, negative-sense RNA viruses.
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Radford, David Robert. "Adherence of #Candida albicans' to denture base materials : the effects of surface finish and dimorphic expression of phenotypically switched organisms." Thesis, King's College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307457.
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Rack, Anita Lena. "Das Periodisches Fieber, Aphthöse Stomatitis, Pharyngitis und Adenitis (PFAPA)-Syndrom." Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-107748.
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Tower, Dallas Lauren. "The effect of putative vesicular stomatitis virus methyltransferase mutants on transcription and replication." [Gainesville, Fla.] : University of Florida, 2005. http://purl.fcla.edu/fcla/etd/UFE0010088.
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Thesis (M.S.)--University of Florida, 2005.Typescript. Title from title page of source document. Document formatted into pages; contains 57 pages. Includes Vita. Includes bibliographical references.
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Burkhart, Christoph. "Characterization of the T helper cell response to vesicular stomatitis virus /." Zürich, 1994. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10799.
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Choi, Woo-Young. "Molecular biological characterization of defective interfering particles of vesicular stomatitis virus." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq26110.pdf.
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Mire, Chad Edward. "Investigation of the vesicular stomatitis virus matrix protein uncoating and assembly /." View the abstract Download the full-text PDF version, 2008. http://etd.utmem.edu/abstracts/2008-035-Mire-index.html.
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Thesis (Ph.D.)--University of Tennessee Health Science Center, 2008.Title from title page screen (viewed on September 9, 2008). Research advisor: Michael A. Whitt, Ph.D. Document formatted into pages (xi, 119 p. : ill.). Vita. Abstract. Includes bibliographical references (p. 104-119).
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D'Avila, Susana [UNESP]. "Avaliação clínica e micológica de pacientes portadores de prótese total superior." Universidade Estadual Paulista (UNESP), 2003. http://hdl.handle.net/11449/97305.
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davila_s_me_arafo.pdf: 393788 bytes, checksum: 2a1483d53614b48a6d0808af22f12f9d (MD5)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Embora a etiologia da estomatite por prótese total seja frequentemente associada à Candida albicans, a literatura também indica que a infecção pode ser associada à outros fatores. Neste ínterim, o objetivo deste estudo foi avaliar a correlação entre a prevalência da estomatite por prótese total, o grupo sanguíneo ABO, presença de Candida ssp. e a higienização da prótese total. Foram avaliados 30 pacienttes (média de idade de 61,13 +-11,96 anos) portadores de prótese total superior, sendo que 23 eram do sexo feminino. O exame clínico incluiu a avaliação da mucosa do palato e a avaliação da higienização da prótese total. A qualificação da higiene da prótese total foi feita por meio da visualização do biofilme com o uso de evidenciadores de placa, nas superfícies internas das próteses. As amostras micológicas foram obtidas da mucosa do palato (região central), da superfície interna da prótese total (região central) e do dorso da língua, por meio da técnica de impressão (imprint), utilizando discos de filtro de papel, colocados diretamente em placas de cultura contendo meio seletivo CHROMagar Candida. As espécies isoladas foram identificadas por meio da prova de produção de clamidoconídeo e pelo sistem API 20C AUX, além da pré-identificação fornecida pelo uso do meio seletivo. Amostra sanguínea foi coletada para determinação do tipo sanguíneo do paciente (sistema ABO e Rh). As análises estatísticas dos resultados foram feitas utilizando testes não paramétricos (Spearman, Mann-Whitney e Kruskal-Wallis). Em nosso estudo, 53,33% dos pacientes apresentaram estomatite por prótese total e a identificação de Candida ssp. foi positiva para 80% dos pacientes. O grau de higiene das próteses...
Although the etiology of denture stomatitis has been commonly related to Candida albicans, some previous studies indicate that the infection could be associated to other factors. The aim of this study was to evaluate the correlation between the prevalence of denture stomatitis, ABO blood groups, mycological status and denture cleanliness. Thirty patients with mean age of 61.13+-11.96 yeas being 23 female using complete maxillary dentures were investigated. Oral examinations included clinical diagnosis of denture stomatitis as well as the evaluation of denture cleanliness. The denture cleanliness was assessed using a stain as plaque detector in the fitting denture surface. The yeast cultures from the palatal mucosa, fitting denture surface and dorsum of the tongue were obtained using an imprint at CHROMagar Candida. The determinations of the species of the isolates was performed by chlamydospore formation and sugar assimilation using API 20C AUX. Venous blood was collected for ABO blood group determination. Spearman correlation, non-parametric t test and analysis of variance were performed to analyse the data. In this study, 53.33% of the patients presented denture stomatitis and the identification of Candida spp. was positive in 80% of the samples. The denture clean liness was classified as poor in 93.33% of the dentures. A statistical correlation was found between yeast detection and denture stomatitis (r=0.8250; p<0,0001) and between Candida ssp. and denture cleanliness (r=0,4666; p=0,0248). Although, majority of the patients were from blood group O no statistical correlation was found between clinical variables and mycological results. Candida albicans was the most frequent yeast isolated (60.94%) followed by C. tropicalis, C. glabrata... (Complete abstract, click electronic address below)
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Le, Bars Pierre. "Etude histo-immunologique de la stomatite prothetique." Nantes, 2000. http://www.theses.fr/2000NANT23VS.
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Källstrand, Jonna, and Maria Lindvall. "Egenvård och behandling vid recidiverande aftös stomatit." Thesis, Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-27233.
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Webb, Bettine Constance. "Laboratory and clinical studies on the treatment of candida-associated denture stomatitis with sodium hypochlorite or microwave irradiation." University of Sydney, 1996. http://hdl.handle.net/2123/4466.
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Doctor of PhilosophyThis thesis describes experiments which were carried out at the Institute of Dental Research in Sydney and within the Department of Prosthetic Dentistry at the United Dental Hospital of Sydney between February 1991 and May 1996. The study is concerned with finding practical means of treating chronic atrophic candidosis, also referred to as Candida-associated denture stomatitis and to this purpose two methods of denture disinfection are investigated, namely, sodium hypoclorite denture soak and microwave irradiation. Although the aetiology of denture stomatitis is generally considered to be multifactorial, there is sufficient evidence that Candida species and in particular C. albicans play an important role in the aetiology of the condition. In Chapter 1, therefore, the literature review, which provides relevant background information for the experiments to be described in later chapters, is primarily concerned with Candida species. The characteristics and distribution of Candida species are described and factors affecting the distribution of or Candida are discussed. The literature relating to the cause of chronic atrophic candidosis is vast and consequently a detailed description is given of Candida-associated denture stomatitis in the section concerned with oral diseases caused by Candida and their treatment. Each of the subsequent chapters, contains a brief literature review of material relevant to the subject of the particular chapter. Chapter 2 describes laboratory work to assess the effect of sodium hypochlorite on the adhesion of Candida species to oral surfaces and the ability of Candida to coaggregate with oral streptococci. The results showed that sodium hypochlorite decreased the ability of Candida species to adhere to both inert surfaces and BECs. However, coaggregation of Candida with streptococci was increased. Thus, hypochlorite if used as a denture soak may initially reduce the ability of Candida species to adhere to the denture surface and may therefore assist the treatment of denture stomatitis. The effects of hypochlorite on the characteristics of Candida species that are associated with tissue invasion are described in Chapter 3. The production of acid proteinase, the formation of germ tubes and presence of major cell wall proteins at 43 and 27 kDa are demonstrated. The ability of the whole cells of certain species of Candida to aggregate human platelets was assessed. The results showed that sodium hypochlorite did not affect proteinase production by Candida species but the rate of germ tube formation and the production of Candida cell wall proteins were increased. Hypochlorite did not affect the ability of certain Candida species to aggregate human platelets. Mechanisms to defend the host against candidal invasion are discussed and include platelet aggregation where aggregated platelets release antimicrobial factors that are active against Candida. Chapter 4 describes an in vitro study to test the effects of sodium hypochlorite and microwave irradiation on the survival of Candida species and oral streptococci on denture surfaces. The results showed that 0.02% sodium hypochlorite denture soak for 8 h will eliminate Candida species and reduce the growth of streptococci. However, microwaving of dentures at medium setting for 6 min will eliminate both Candida and streptococci. This information servers as baseline data for clinical assessments described in Chapters 7 and 8. Denture hygiene is an important factor in the prevention and treatment of Candida-associated denture stomatitis. Hence, a clinical study to assess the microbiology of denture plaque is described in Chapter 5. The results showed that denture plaque was composed mainly of Gram-positive streptococci with varying proportions of Gram-positive rods, Gram-negative cocci and rods and is similar to dental plaque. Candida was not always isolated and when detected constituted a very small proportion (< 1%) of the total aerobic bacterial count. The results of an investigation to test the effect of soft denture liners in lower dentures on the colonization of denture surfaces by Candida species and aerobic bacteria are given in Chapter 6. There was no significant difference in Candida /bacterial colonization of dentures with soft denture liners and those without liners. Chapter 7 describes a clinical study to test the efficiency of sodium hypochlorite (0.02%) over-night denture soak as an effective denture disinfecting agent. Treatment of dentures with hypochlorite over a trial period resulted in reductions of Candida and aerobic bacteria and although the reductions were not significant the effect over the trial period could be assessed. A significant finding was that for the palate, treatment with hypochlorite over the trial period prevented an increase in candidal load. Thus, sodium hypochlorite may function as an effective disinfecting agent when used as 0.02% denture soak for a prolonged period. A pilot study to assess the effectiveness of microwaving dentures for ten min (350 W, 240 MHz) as a potential method of denture disinfection is described in Chapter 8. For practical reasons the dentures were microwaved only once only and therefore the effect over a trial period could not be assessed. However, one treatment resulted in significant reductions in the levels of Candida and aerobic bacteria. These findings have indicated that future research should be carried out to test the effect of daily consecutive microwave treatments on candidal and bacterial growth. The general discussion in Chapter 9 summarizes the data presented in the previous chapters and from the findings conclusions are made concerning the prevention and treatment of Candida-associated denture stomatitis. The limitations of this thesis are recognized and some important aspects of the study are recommended for future research.
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Sanitá, Paula Volpato. "Estomatite protética em pacientes com Diabetes mellitus : prevalência de Candida spp. e eficiência dos tratamentos com nistatina e desinfecção de próteses por micro-ondas /." Araraquara, [s.n.], 2011. http://hdl.handle.net/11449/105514.
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Orientador: Carlos Eduardo VerganiBanca: Marco Antonio Compagnoni
Banca: Silvana Regina Perez Orrico
Banca: Helena de Freitas Oliveira Paranhos
Banca: Karin Hermana Neppelenbroek
Resumo: Este estudo in vivo teve como objetivos avaliar: 1. a prevalência das diferentes espécies de Candida em pacientes diabéticos portadores de estomatite protética e compará-la àquela de pacientes não diabéticos com/sem estomatite protética; 2. a eficiência de dois tratamentos para estomatite protética em pacientes diabéticos: terapia antifúngica com nistatina e desinfecção de próteses totais por micro-ondas. Para isso, 210 pacientes usuários de prótese totais foram selecionados e distribuídos em três grupos de estudo. O grupo controle - GC foi formado por 90 pacientes não diabéticos e com mucosa oral saudável; os grupos experimentais foram formados por portadores de estomatite protética, sendo 80 pacientes não diabéticos - EPND e 40 diabéticos do tipo 2 controlados - EPD. Uma avaliação intra-oral dos pacientes dos grupos experimentais foi realizada e a aparência clínica da mucosa inflamada foi classificada de acordo com os critérios de Newton em graus I, II ou III. Coletas microbiológicas das próteses totais foram realizadas com swab estéril e plaqueadas em meio CHROMagar Candida. Em seguida, os procedimentos de identificação foram realizados: análise de microcultivo, teste de triagem fenotípica em caldo hipertônico e testes bioquímicos (ID-32C). Os resultados obtidos foram analisados por Intervalo de Confiança de Bonferroni e testes Exato de Fisher e Qui Quadrado (α=0,05). Os 40 pacientes diabéticos foram, então, submetidos ao tratamento da estomatite protética. Para isso, foram aleatoriamente distribuídos em 2 grupos (n=20): grupo NIS - tratamento com antifúngico tópico nistatina (suspensão oral - 100.000UI/mL), 4 vezes ao dia, por 14 dias; grupo MIC - tratamento por meio da irradiação das totais por micro-ondas (650W / 3 minutos), 3 vezes por semana, por 14 dias. Dois parâmetros... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The aim of this in vivo study was to evaluate: 1. the prevalence of Candida spp. in well-controlled type 2 diabetic patients and compare it to that found in non-diabetics with/without denture stomatitis; 2. the efficacy of two treatments of denture stomatitis in diabetic patients: topical nystatin and denture microwave disinfection. Two hundred and ten denture wearer patients were divided into three groups of study. The control group - CG was formed by 90 individuals without diabetes or denture stomatitis and the experimental groups were formed by patients with denture stomatitis, being 80 non-diabetics - DSND and 40 well-controlled type 2 diabetics - DSD. Mucosal characteristics of denture stomatitis patients were classified in types I, II, and III, according to Newton's criteria. Mycological samples were taken from the dentures and cultured in CHROMagar′s plates. Candida spp. were identified by micro-cultivation, hypertonic Sabouraud broth, and bioMérieux ID32C assays. Results were analyzed by means of Bonferroni corrected confidence interval, Fisher's exact test, and Chi-square analysis of several proportions (α=0.05). The 40 diabetic patients were divided into two groups (n=20) and submitted to two treatments for denture stomatitis: NYS group - patients were treated with topical nystatin, 4 times a day, for 14 days; MW group - patients had their dentures microwaved (650W / 3 minutes), 3 times per week, for 14 days. Mycological samples were taken from the palates and the tissue surface of the dentures for quantification and identification of Candida spp. and standardized photographs of the palates were taken for the clinical analysis. Microbiological and clinical evaluations were repeated at baseline... (Complete abstract click electronic access below)
Doutor
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D'Avila, Susana. "Avaliação clínica e micológica de pacientes portadores de prótese total superior /." Araraquara : [s.n.], 2003. http://hdl.handle.net/11449/97305.
Full textAbstract:
Resumo: Embora a etiologia da estomatite por prótese total seja frequentemente associada à Candida albicans, a literatura também indica que a infecção pode ser associada à outros fatores. Neste ínterim, o objetivo deste estudo foi avaliar a correlação entre a prevalência da estomatite por prótese total, o grupo sanguíneo ABO, presença de Candida ssp. e a higienização da prótese total. Foram avaliados 30 pacienttes (média de idade de 61,13 +-11,96 anos) portadores de prótese total superior, sendo que 23 eram do sexo feminino. O exame clínico incluiu a avaliação da mucosa do palato e a avaliação da higienização da prótese total. A qualificação da higiene da prótese total foi feita por meio da visualização do biofilme com o uso de evidenciadores de placa, nas superfícies internas das próteses. As amostras micológicas foram obtidas da mucosa do palato (região central), da superfície interna da prótese total (região central) e do dorso da língua, por meio da técnica de impressão ("imprint"), utilizando discos de filtro de papel, colocados diretamente em placas de cultura contendo meio seletivo CHROMagar Candida. As espécies isoladas foram identificadas por meio da prova de produção de clamidoconídeo e pelo sistem API 20C AUX, além da pré-identificação fornecida pelo uso do meio seletivo. Amostra sanguínea foi coletada para determinação do tipo sanguíneo do paciente (sistema ABO e Rh). As análises estatísticas dos resultados foram feitas utilizando testes não paramétricos (Spearman, Mann-Whitney e Kruskal-Wallis). Em nosso estudo, 53,33% dos pacientes apresentaram estomatite por prótese total e a identificação de Candida ssp. foi positiva para 80% dos pacientes. O grau de higiene das próteses... (Resumo completo, clicar acesso eletrônico abaixo)Abstract: Although the etiology of denture stomatitis has been commonly related to Candida albicans, some previous studies indicate that the infection could be associated to other factors. The aim of this study was to evaluate the correlation between the prevalence of denture stomatitis, ABO blood groups, mycological status and denture cleanliness. Thirty patients with mean age of 61.13+-11.96 yeas being 23 female using complete maxillary dentures were investigated. Oral examinations included clinical diagnosis of denture stomatitis as well as the evaluation of denture cleanliness. The denture cleanliness was assessed using a stain as plaque detector in the fitting denture surface. The yeast cultures from the palatal mucosa, fitting denture surface and dorsum of the tongue were obtained using an imprint at CHROMagar Candida. The determinations of the species of the isolates was performed by chlamydospore formation and sugar assimilation using API 20C AUX. Venous blood was collected for ABO blood group determination. Spearman correlation, non-parametric t test and analysis of variance were performed to analyse the data. In this study, 53.33% of the patients presented denture stomatitis and the identification of Candida spp. was positive in 80% of the samples. The denture clean liness was classified as poor in 93.33% of the dentures. A statistical correlation was found between yeast detection and denture stomatitis (r=0.8250; p<0,0001) and between Candida ssp. and denture cleanliness (r=0,4666; p=0,0248). Although, majority of the patients were from blood group O no statistical correlation was found between clinical variables and mycological results. Candida albicans was the most frequent yeast isolated (60.94%) followed by C. tropicalis, C. glabrata... (Complete abstract, click electronic address below)
Orientador: Maria Regina Sposto
Coorientador: Maria José Soares Mendes Giannini
Banca: Carlos Eduardo Vergani
Banca: Jacks Jorge Junior
Mestre
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Heiber, Joshua F. "Characterization and Development of Vesicular Stomatitis Virus For Use as an Oncolytic Vector." Scholarly Repository, 2011. http://scholarlyrepository.miami.edu/oa_dissertations/600.
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Oncolytic virotherapy is emerging as a new treatment option for cancer patients. At present, there are relatively few oncolytic virus clinical trials that are underway or have been conducted, however one virus that shows promise in pre-clinical models is Vesicular Stomatitis Virus (VSV). VSV is a naturally occurring oncolytic rhabdovirus that has the ability to preferentially replicate in and kill malignant versus normal cells. VSV also has a low seroprevalence, minimal associated morbidity and mortality in humans, and simple non-integrating genome that can be genetically manipulated, making it an optimal oncolytic vector. Currently, many labs are using a variety of different strategies including inserting trans genes that can modulate the innate and adaptive immune response. VSV can also be retargeted by altering its surface glycoprotein (G) or be made replication incompetent by deleting the G protein. Currently, our lab has engineered a series of new recombinant VSVs, incorporating either the murine p53 (mp53), IPS-1, or TRIF transgene. mp53, IPS-1 and TRIF were incorporated into the normal VSV-XN2 genome and mp53 was also incorporated into the mutated VSV-ΔM vector generating VSV-mp53, VSV-IPS-1, VSV-TRIF and VSV-ΔM-mp53. Our data using these new viruses indicate that these viruses preferentially replicate in and kill transformed versus non-transformed cells and efficiently express the transgene. However, despite the ability for VSV-IPS-1 and VSV-TRIF to induce a robust type 1 IFN response, VSV-ΔM-mp53 was the only construct that had reduced toxicity and elicited an increased anti-tumor response against a syngeneic metastatic mammary tumor model. VSV- ΔM-mp53 treatment lead to a reduction in IL-6 and IP-10 production, an increase in tumor specific CD8+ T cells, and immunologic memory against the tumor. Collectively these studies highlight the necessity for additional VSV construct development and the generation of new clinically relevant treatment schema.
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Edge, Robert E. "Exploiting Differential Endogenous MicroRNA Expression to Enhance Oncolytic Vesicular Stomatitis Virus Tumour Tropism." Thesis, Université d'Ottawa / University of Ottawa, 2010. http://hdl.handle.net/10393/19578.
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The creation of potent oncolytic viruses (OVs) suitable for the clinic may require new strategies in virus design. Replication-competent viruses facilitate a variety of approaches to achieving tumor specificity. Altered expression of microRNAs is a common hallmark of cancer that this thesis demonstrates can be used to alter expression of a potent wild-type viral gene to achieve tumor-specific replication of an engineered vesicular stomatitis virus (VSV). Incorporation of microRNA complementary sequences (miRTs) within VSV causes reduced accumulation of miRT containing mRNAs. Let-7 miRTs introduced into the 3' untranslated region (3'UTR) of VSV matrix protein mRNA eliminate undesirable replication and associated toxicity in normal cells but permit growth in cancer cells both in vitro and in vivo. Similarly the incorporation of mir34a miRTs results in reduced in vitro VSV replication and cytotoxicity in the presence of activated p53. This thesis provides proof of concept that viruses designed to exploit the differential microRNA expression in cancer cells is a viable approach, potentially useful in optimizing oncolytic viral gene expression for maximal antitumor activity and safety.
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Mead, Daniel G. "Maintenance and transmission of vesicular stomatitis viruses: New data for an old puzzle." Diss., The University of Arizona, 1999. http://hdl.handle.net/10150/284067.
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Insect and rodent samples were collected from suspected VSV-NJ enzootic areas over 2 consecutive field seasons (1996-1997) and from southern Arizona only during 1998. Insect samples were screened for arboviruses, and rodent sera were tested for the presence of VSV-NJ and VSV-IN neutralizing antibodies. Vesicular stomatitis virus New Jersey serotype was isolated from a pool of Culicoides sp. collected in 1997 near Belen, New Mexico. All rodent sera were negative for specific VSV-NJ and VSV-IN antibodies. Genetic analysis of the hypervariable region of the phosphoprotein gene demonstrated that the 1997 Belen VSV-NJ isolate was more closely related to viruses isolated from livestock during the 1982-83 western U.S. epizootic than to other VSV-NJ isolates. This suggests that VSV-NJ may be enzootic in the western U.S. Simulium vittatum was shown to be a competent vector of VSV-NJ. Virus-infected females were allowed to feed on laboratory mice and on deer mice. All laboratory mice seroconverted by day 21 post-exposure. Neutralizing antibody titers increased from an average of 1:4 at baseline to >1:1,024 on day 21. An age-related effect on viral pathogenesis was evident in Peromyscus maniculatus following VSV-NJ exposure by black fly bite. Lethal encephalomyelitis was evident in all 6-week-old mice, but in only one 6-month-old mouse. Peromyscus maniculatus did not meet the standard definition of a reservoir host for VSV-NJ because a viremia, was not detected. Nonetheless, P. maniculatus may play a role in virus maintenance since non-infected black flies became infected while co-feeding with infected black flies on the same non-viremic host. These results represent the first example of a western U.S. insect species becoming infected with VSV-NJ by feeding on a host. Simulium vinatum and S. notatum were shown to be competent laboratory vectors of VSV-IN. Saliva from experimentally infected Simulium vittatum and S. notatum was collected and tested for the presence of infectious virus. Virus was detected in the saliva of both species following oral infection. Independent experiments were conducted to determine if transovarial transmission of VSV-NJ and VSV-IN occurs in black flies. Transovarial transmission was not detected. Transstadial transmission of both virus serotypes was detected.
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Knowles, J. "Studies on feline calicivirus with particular reference to chronic stomatitis in the cat." Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382065.
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Ostertag, Derek Glenn. "Novel dsRNA-dependent activation of a cellular antiviral response to vesicular stomatitis virus /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2005. http://wwwlib.umi.com/cr/ucsd/fullcit?p3167840.
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Coco, Brent Jason. "Antifungal resistance and pathogenesis of Candida albicans biofilms in patients with denture stomatitis." Thesis, Glasgow Caledonian University, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.520978.
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Ferlin, Anna. "Characterization of vesicular stomatitis virus of glycoprotein mutants affected in their structural transition." Paris 7, 2013. http://www.theses.fr/2013PA077262.
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Les structures pré- et post-fusion de G VSV ont été élucidées par radiocristallographie,permettent d'identifier certains résidus qui pourraient jouer le rôle d'interrupteurs moléculaires sensibles au pH depuis la forme post- vers la forme pré-fusion. Ces acides aminés acides ont donc été remplacés par de simples(D268L,N,V;D274N;E276Q;D393N;D395N: et doubles mutants(D274N-D395N,E276-393N). L'expression,repliement,transport à la membrane cellulaire ont été analysés par immunofluorescence et FACS. Des expériences de fusion cellule-cellule ont montré que la plupart de ces mutants sont affectés dans leur propriétés de fusion. En particulier,les mutants en position 268 ont complètement perdu leur activité de fusion. Les mutants D268V/L sont piégés dans leur conformation post-fusion alors que la mutation D268N entraîne la création d'un site de glycosylation,qui rend le mutant incapable de former le trimère post-fusion. Tous ces mutants,sauf E276Q,ont des capacités réduites à pseudotyper un virus dépourvu du gène de la G. De façon inattendue,la stabilisation du trimère post-fusion empêche l'incorporation de G dans les particules virales. Nous avons utilisé la génétique inverse pour produire des particules virales recombinantes. La plupart des mutants on immédiatement réverté vers la séquence de type sauvage révélant l'importance de celle-ci. Ces données démontrent que les résidus identifiés précédemment sont bien des régulateurs du changement de conformation de G et que D268 est k résidu majeur dans cet rôle. Elles suggèrent aussi que la conformation de G régule son incorporation dans les particule naissantes et/ou la libération de la nucléocapside virale dans le cytoplasme après fusionThe structure of the pre- and post-fusion of VSV G states have been elucidated by X-ray crystallography,allowing the identification of some amino acid residues which could play the role of pH-dependent molecular switches,triggering the conformational change from the post- toward the pre-fusion state. To confirm this,these acidic amino acids were mutated,creating single(D268L,N,V;D274N;E276Q;D393N;D395N) and double mutants (D274N-D395N,E276Q-D393N). Their expression,folding and transport to the tell membrane were analyzed by immunofluorescence and FACS. A cell-cell fusion assay showed that most of these mutants are affected in their membrane fusion properties. Particularly,mutants D268L/V/N have completely lost their fusion activity. We demonstrated that D268LN are trapped in their post-fusion state whereas the mutation D268N,which results in the creation of an efficient glycosylation, is unable to form the post fusion trimer. All these mutants, except mutant E276Q, do not or very inefficiently pseudotype a virus lacking the glycoprotein gene. Surprisingly,the stabilization of the post fusion trimer precludes G incorporation into the viral particles. We used a reverse genetic approach to produce recombinant viral particles. Most of the mutants immediately reverted toward the wild type sequence indicating the importance of the wild type sequence in this region. These data demonstrate that the residues identified are indeed regulators of G conformational change and that D268 is the major pH sensitive switch. It also suggests that the conformation of G regulates its incorporation in nascent particles and/or the release of viral nucleocapsid into the cytoplasm alter fusion
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Paterson, Jennifer Marie. "Investigation of the oncolytic activity of vesicular stomatitis virus in murine cancer models." Thesis, University of Ottawa (Canada), 2005. http://hdl.handle.net/10393/27005.
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Interferon (IFN) inducing strains of Vesicular stomatitis virus (VSV) replicate selectively in transformed cells and exert impressive oncolytic abilities in murine tumour models. Interestingly, in some tumour models, complete tumour cures can only be obtained by administering multiple intravenous doses of virus, indicating that there must be barriers that limit the ability of a single dose of virus to eliminate a tumour. The goal of the current research was to examine the intravenous delivery of IFN inducing VSV to subcutaneous tumours, as well as subsequent spread and tumour killing activity. It was found that intravenous delivery is inefficient and seems to result in preferential delivery of virus to the tumour rim. However, apoptosis is induced throughout the uninfected core of the tumour, indicating that VSV may induce indirect killing of tumour cells. While the specific cause of this apparent bystander effect was not determined, it was observed that VSV treatment also resulted in collapse of functional tumour vasculature.
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馮婧宇. "放射性口腔炎的中醫證治規律研究及其中藥臨床隨機對照試驗的系統評價." HKBU Institutional Repository, 2012. http://repository.hkbu.edu.hk/etd_ra/1343.
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Sudini, Kuladeep Reddy. "A Role for Interferon stimulated gene 12a (ISG12a) in vesicular stomatitis virus antiviral responses." University of Toledo / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1343098364.
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Mao, Jian. "Vesicular stomatitis virus G pseudotyped retrovector mediated gene delivery of connexin43 to brain tumor cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0001/MQ42173.pdf.
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吳苑汶 and Yuen-man Ng. "Evidence-based intervention protocol of using ice water mouthwash in the prevention of stomatitis for patients undergoing autologous haematological stem cell transplantation." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193034.
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Haematological stem cell transplantation (HSCT) is a revolutionary treatment for haematological malignancies. Although HSCT is potentially curative, patients usually develop stomatitis which is a common and debilitating complication after the transplantation. Furthermore, stomatitis may predispose patients to various complications which are associated with significantly increased morbidity and mortality.
In some studies, ice water mouthwash has been shown to be an effective method for the prevention of stomatitis. However, a high-level evidence-based protocol on the prevention of stomatitis has not been fully developed and it is not commonly practiced in most HSCT centers at present. A well established protocol can help to minimize the patients’ suffering and avoid prolonged hospitalization. The nurses who are involved in patient education, assessment, care for, and coping with stomatitis, play an important role to bring these innovations into practice.
In this regard, this translational research aims at developing an evidence-based protocol on using ice water mouthwash in the prevention of stomatitis for patients undergoing autologous HSCT. A systematic search for relevant literatures was performed with the use of five electronic databases. Six relevant studies were found. Critical appraisal on the relevant studies was conducted. The level of evidence extracted from the studies was graded according to the Scottish Intercollegiate Guidelines Network (SIGN) and were synthesized to establish the protocol for patients in the proposed setting. The implementation potential of the protocol was assessed in terms of the transferability, feasibility, and cost benefit ratio. An implementation and evaluation plan was established for comprehensive evidence-based protocol development.
The successful implementation of the protocol will be beneficial for the patients undergoing HSCT as it may hasten their recovery, shorten their hospital stay, and minimize their distressing experience and suffering.published_or_final_version
Nursing Studies
Master
Master of Nursing
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Matheny, Elizabeth Lane. "Contribution of the membrane-proximal region of the vesicular stomatitis virus gycoprotein to host cell entry and membrane fusion." View the abstract Download the full-text PDF version, 2009. http://etd.utmem.edu/ABSTRACTS/2009-043-Matheny-index.htm.
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Thesis (Ph.D.)--University of Tennessee Health Science Center, 2009.Title from title page screen (viewed on February 3, 2010). Research advisor: Michael A. Whitt, Ph.D. Document formatted into pages (x, 91 p. : ill.). Vita. Abstract. Includes bibliographical references (p. 81-90).
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Sanitá, Paula Volpato [UNESP]. "Estomatite protética em pacientes com Diabetes mellitus: prevalência de Candida spp. e eficiência dos tratamentos com nistatina e desinfecção de próteses por micro-ondas." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/105514.
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sanita_pv_dr_arafo.pdf: 1953204 bytes, checksum: a2f3ce08a1a45c4435513d02ab367cba (MD5)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Este estudo in vivo teve como objetivos avaliar: 1. a prevalência das diferentes espécies de Candida em pacientes diabéticos portadores de estomatite protética e compará-la àquela de pacientes não diabéticos com/sem estomatite protética; 2. a eficiência de dois tratamentos para estomatite protética em pacientes diabéticos: terapia antifúngica com nistatina e desinfecção de próteses totais por micro-ondas. Para isso, 210 pacientes usuários de prótese totais foram selecionados e distribuídos em três grupos de estudo. O grupo controle - GC foi formado por 90 pacientes não diabéticos e com mucosa oral saudável; os grupos experimentais foram formados por portadores de estomatite protética, sendo 80 pacientes não diabéticos - EPND e 40 diabéticos do tipo 2 controlados - EPD. Uma avaliação intra-oral dos pacientes dos grupos experimentais foi realizada e a aparência clínica da mucosa inflamada foi classificada de acordo com os critérios de Newton em graus I, II ou III. Coletas microbiológicas das próteses totais foram realizadas com swab estéril e plaqueadas em meio CHROMagar Candida. Em seguida, os procedimentos de identificação foram realizados: análise de microcultivo, teste de triagem fenotípica em caldo hipertônico e testes bioquímicos (ID-32C). Os resultados obtidos foram analisados por Intervalo de Confiança de Bonferroni e testes Exato de Fisher e Qui Quadrado (α=0,05). Os 40 pacientes diabéticos foram, então, submetidos ao tratamento da estomatite protética. Para isso, foram aleatoriamente distribuídos em 2 grupos (n=20): grupo NIS – tratamento com antifúngico tópico nistatina (suspensão oral - 100.000UI/mL), 4 vezes ao dia, por 14 dias; grupo MIC – tratamento por meio da irradiação das totais por micro-ondas (650W / 3 minutos), 3 vezes por semana, por 14 dias. Dois parâmetros...
The aim of this in vivo study was to evaluate: 1. the prevalence of Candida spp. in well-controlled type 2 diabetic patients and compare it to that found in non-diabetics with/without denture stomatitis; 2. the efficacy of two treatments of denture stomatitis in diabetic patients: topical nystatin and denture microwave disinfection. Two hundred and ten denture wearer patients were divided into three groups of study. The control group - CG was formed by 90 individuals without diabetes or denture stomatitis and the experimental groups were formed by patients with denture stomatitis, being 80 non-diabetics - DSND and 40 well-controlled type 2 diabetics - DSD. Mucosal characteristics of denture stomatitis patients were classified in types I, II, and III, according to Newton’s criteria. Mycological samples were taken from the dentures and cultured in CHROMagar′s plates. Candida spp. were identified by micro-cultivation, hypertonic Sabouraud broth, and bioMérieux ID32C assays. Results were analyzed by means of Bonferroni corrected confidence interval, Fisher’s exact test, and Chi-square analysis of several proportions (α=0.05). The 40 diabetic patients were divided into two groups (n=20) and submitted to two treatments for denture stomatitis: NYS group – patients were treated with topical nystatin, 4 times a day, for 14 days; MW group – patients had their dentures microwaved (650W / 3 minutes), 3 times per week, for 14 days. Mycological samples were taken from the palates and the tissue surface of the dentures for quantification and identification of Candida spp. and standardized photographs of the palates were taken for the clinical analysis. Microbiological and clinical evaluations were repeated at baseline... (Complete abstract click electronic access below)
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Günes, Serap. "MODIFICATION OF VESICULAR STOMATITIS VIRUS G PROTEIN FOR TARGETED GENE DELIVERY INTO PSCA-POSITIVE TUMOR CELLS." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2007. http://nbn-resolving.de/urn:nbn:de:swb:14-1182861723404-04537.
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Gene therapy is a promising treatment option for cancer. Ideally, a therapeutic gene is delivered specifically into tumor cells sparing the neighboring normal cells. For this purpose gene delivery vectors are designed that can recognize structures, which are exclusively expressed on tumor cells (i.e. the tumor-associated antigens -TAA-). Retroviral vectors are commonly used for gene therapy by modifying the envelope protein responsible for the recognition of the target cell. The Vesicular Stomatitis Virus G protein (VSV-G) is a well-liked choice for pseudotyping the retroviral vectors since it confers on the viral particle stability to allow concentration to high titers necessary for the clinical applications. However, the main drawback of VSV-G, the ubiquitously expressed receptor and thus the broad target range, hinders the use of this protein for targeted gene therapy. In this thesis, we aimed to modify the VSV-G for targeted gene therapy against Prostate Stem Cell Antigen (PSCA) -expressing tumors. Therefore we followed two approaches. The first approach comprised of the fusion of a single-chain antibody fragment against PSCA to the N-terminus of VSV-G. In the second approach the VSV-G was modified by insertion of a small epitope. We could demonstrate that two positions in the N-terminal region of VSV-G protein permit insertion of a ten amino acid long epitope. These mutant VSV-G proteins were successfully assembled into retroviral particles. We demonstrated that the mutant retroviral particles can be used for targeting to PSCA-positive cells using nanobeads. The nanobeads were chemically coupled to antibodies against the epitope in the VSV-G protein and PSCA on the tumor cell. These bispecific nanobeads allowed the recruitment of mutant retroviral particles to the PSCApositive cells. Our results point out the potential of these mutant retroviral particles in targeted gene delivery. Further studies will be necessary to assess the efficiency of in vivo targeted gene therapy using these mutant retroviral particles.
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Shinde, Prashant [Verfasser]. "Tumor necrosis factor mediated augmentation of immunity protects host against Vesicular stomatitis virus infection / Prashant Shinde." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2019. http://d-nb.info/1188882368/34.
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Burson, Kim Katrina. "Gene shuffling of bacterial aromatic polyketide synthases ; Binding and fusion studies of vesicular stomatitis virus (VSV) /." May be available electronically:, 1998. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.
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Thesis (Ph. D.)--Stanford University, 1998.Submitted to the Department of Chemistry. Copyright by the author. No collective title. Part 1 Gene shuffling of bacterial aromatic polyketide synthases. Chapters 1-3. Part 2 Binding and fusion studies of vesicular stomatitis virus (VSV). Chapters 4-5.
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Günes, Serap. "Modifikation of vesicular stomatitis virus G protein for targeted gene delivery into PSCA-positive tumor cells." [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:swb:14-1182861723404-04537.
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Danet, Nicolas. "Molecular characterisation of the recombinant Vesicular Stomatitis Virus- ZEBOV-GP virus, prototype vaccine against Ebola virus." Thesis, Lyon, 2019. http://www.theses.fr/2019LYSE1009/document.
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Ebolavirus (EBOV) est un filovirus responsable de fièvres hémorragiques virales sévères chez l’humain, qui peuvent être létales dans 90% des cas. L’actuelle épidémie en République Démocratique du Congo et l’ampleur démesurée de l'épidémie de 2014-2016 en Afrique de l’Ouest, qui a causé la mort de plus de 11 000 personnes, ont poussé les agences sanitaires internationales à tester plusieurs approches thérapeutiques afin d’essayer d’endiguer rapidement la propagation virale et de limiter la mortalité liée au virus lors de futures épidémies. Parmi toutes les stratégies testées, le virus recombinant réplicatif rVSV-ZEBOV qui exprime la glycoprotéine de surface d’EBOV, semble offrir la meilleur protection, aussi bien en modèle animaliers que sur le terrain. Avant d’être testé chez l’humain, de nombreuses études ont permis de mettre en évidence l’efficacité et l’innocuité de ce vaccin prototype. Pourtant et malgré le fait que de nombreuses études ont démontré l’importance et le rôle de la glycoprotéine GP dans l’efficacité des vaccins contre ce virus, aucune étude n’a encore été réalisé sur la nature des glycoprotéines virales synthétisées par le gène GP d’EBOV inséré dans le génome du virus VSV. Ainsi, les caractérisations moléculaires des protéines virales produites lors de l’infection par le virus rVSV-GP décrites dans ces travaux de thèse offrent de nouvelles perspectives pour comprendre le succès de ce vaccin mais aussi l’origine virales dans les effets secondaires sévères observés lors de la vaccination, et pourront aider à développer un vaccin plus sûr, qui n’est actuellement pas utilisable chez les personnes immunodépriméesThe filovirus Ebolavirus (EBOV) is the causative agent of severe viral hemorrhagic fevers in humans that can be lethal in 90% of cases. The current outbreak in the Democratic Republic of Congo and the extraordinary scale of the 2014-2016 outbreak in West Africa, that caused the death of more than 11 000 disease victims, lead the international public health agencies to test several therapeutic approach to limit viral spreading and mortality. Amongst those, the recombinant replication-competent rVSV-ZEBOV virus, that expressed EBOV GP glycoprotein, appears to offer the best protection in animal models and outbreak settings. While its effectiveness and safety have been widely investigated before human trials and despite numerous studies that showed the importance the nature of the glycoproteins which are produced during the infection from the EBOV GP gene that has been inserted in VSV genome are unknown. In this respect, the molecular characterisations of the viral glycoproteins synthesised during rVSV-GP presented in this thesis, offer new insights with which to understand the success of the rVSV-GP vaccine but also the potential viral origins of the severe adverse side effects observed during vaccination and could help in developing a safer vaccine, which currently cannot be used in an immunocompromised population
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Samuel, Sara. "Vesicular stomatitis virus and BCL-2 inhibitor combination therapy for the treatment of chronic lymphocytic leukemia." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=119339.
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Chronic lymphocytic leukemia (CLL) is a cancer of the white blood cells (B cell lymphocytes). It is an indolent disorder that results in the accumulation of CD5+ B cells. In CLL, resistance to cell death is attributed to the overexpression of several key pro-survival proteins (i.e. B-cell lymphoma 2 (Bcl-2) and myeloid cell leukemia- 1 (Mcl-1)) that belong to the apoptotic Bcl-2 family of proteins. Bcl-2 and Mcl-1 overexpression deregulates both the apoptotic and autophagic signaling pathways and contributes to tumorigenesis. Oncolytic virotherapy has emerged as a novel anti-cancer therapy for the treatment of a variety of malignant disorders. Oncolytic virus (OV) Vesicular stomatitis virus (VSV)-AV1 takes advantage of genetic defects present within cancerous cells and preferentially targets and kills them. Normal cells are spared the cytotoxic effects of viral lysis and are left unharmed. CLL cells are largely resistant to VSV oncolysis due to an elevated protein expression level of Bcl-2 and Mcl-1 as well as the inhibitory interactions Bcl-2 and Mcl-1 form with pro-apoptotic and pro-autophagic proteins. It is a common approach to use combination therapy to overcome limitations with single agent treatment. In the studies presented within, VSV-AV1 treatment was combined with small-molecule Bcl-2 inhibitors as a means to overcome resistance to oncolytic virtotherapy observed in CLL patients.In the first strategic approach, we combined low-dose amounts of the pan–Bcl-2 inhibitor, Obatoclax, with VSV-AV1 and examined the effect on the apoptotic signaling pathway. Obatoclax and VSV-AV1 synergistically enhanced cell death in primary CLL cells. The combination therapy induced intrinsic apoptotic signaling through the activation of caspases-3 and -9 cleavage. Inhibitory complexes between Bcl-2:Bax and Mcl-1:Bak were disrupted as well. Pro-death protein, Noxa, was upregulated following VSV-AV1 infection and as identified as a critical mediator of apoptotic cell death..In the second approach, we examined VSV-AV1 virotherapy in combination with Bcl-2 inhibitors (Obatolcax or ABT-737) to elucidate the role of the autophagic pathway on cell death in primary CLL cells. We also investigated the crosstalk between the autophagic and apoptotic pathways following combination treatment. Bcl-2 inhibitor/VSV-AV1 therapy led to increased LC3-II and reduced p62 proteins levels, which signify the activation of autophagy. Inhibition of autophagy, with 3-methyladenine, significantly increased apoptotic cell death induced by Bcl-2 inhibitor/VSV-AV1 treatment. The combination therapy also abrogated Bcl-2:Beclin interactions thus stimulating the induction of autophagy.Altogether, our therapeutic strategies indicate that Bcl-2 inhibitors improve VSV-AV1 oncolysis in treatment-resistant hematological malignancies, such as CLL, with characterized defects in apoptotic and autophagic responses.La leucémie lymphoïde chronique (LLC) est un cancer qui affecte les globules blancs et provient de l'accumulation des lymphocytes B CD5+. La résistance à la mort cellulaire est attribuée à la surexpression de plusieurs protéines de pro-survie tel que « B-cell lymphoma 2 » (Bcl-2) et « myeloid cell leukemia (Mcl-1) » qui appartiennent à la famille de protéines apoptotiques Bcl-2. La surexpression de Bcl-2 et Mcl-1 dérégule les voies de signalisations apoptotiques et autophagiques et contribue au développement de la tumeur. La virothérapie oncolytique a démontré son efficacité comme nouvelle thérapie anticancéreuse pour le traitement de plusieurs types de tumeur. Le virus de la stomatite vésiculaire (VSV)-AV1 est un virus oncolytique qui prend avantage des anomalies génétiques présentes dans les cellules cancéreuses pour préférentiellement infecter et détruire ces dernières tandis que les cellules normales sont épargnées de la lyse virale. Cependant, les cellules affectées par LLC sont tout de même résistante à l'oncolyse par VSV à cause d'un niveau d'expression élevé des protéines Bcl-2 et Mcl-1, et de leur effet inhibiteur sur les protéines pro-apoptotiques et pro-autophagiques. Les thérapies combinées permettent de dépasser les limites imposées par les traitements utilisant un seul agent anticancéreux. Dans la présente étude, le traitement oncolytique VSV-AV1 a été combiné avec un autre agent anticancéreux, une molécule inhibitrice de Bcl-2, pour surpasser la résistance au traitement oncolytique par VSV-AV1. Dans une première stratégie, nous avons combiné une faible dose d'Obatoclax, un inhibiteur de Bcl-2, avec VSV-AV1 et examiné l'effet sur la voie de signalisation apoptotique. Obatoclax et VSV-AV1 ont augmenté de façon synergique la mort cellulaire des cellules primaires isolées de patients atteints de LLC. La thérapie combinée a induit la signalisation apoptotique intrinsèque en activant la caspase-3 et caspase-9 ainsi qu'en séparant les complexes inhibiteurs Bcl-2:Bax et Mcl-1:Bax. De plus, l'expression de la protéine pro-apoptotique Noxa a augmenté suite à l'infection avec VSV-AV1 et il a été démontré que cet événement est critique pour enclencher la mort cellulaire par apoptose. Dans une deuxième stratégie, nous avons examiné le rôle de la voie de signalisation de l'autophagie dans la mort cellulaire des cellules primaire LLC suite à la thérapie oncolytique avec VSV-AV1 en combinaison avec Obatoclax ou ABT-737, deux inhibiteurs de Bcl-2. Nous avons étudié l'interaction entre l'autophagie et l'apoptose suite au traitement combiné et démontré que le traitement a augmenté le niveau de LC3-II et a réduit le niveau de la protéine p62, ce qui indique une activation de l'autophagie. En contrepartie, l'inhibition de l'autophagie avec le 3-methyladénine a augmenté de façon significative la voie apoptotique. La thérapie combinée a également bloqué l'interaction entre Bcl-2 et Bectin et par conséquent stimulé l'induction de l'autophagie. Les stratégies thérapeutiques examinées dans cette étude indiquent que les inhibiteurs de Bcl-2 améliorent l'oncolyse virale dans les leucémies résistantes aux traitements simples et qui sont caractérisées par des anomalies dans la réponse apoptotique et autophagique, tel qu'observé chez les patients atteints de LLC.
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Ferrisse, Túlio Morandin [UNESP]. "Análise das características clínica, histopatológica e imunopatológica das lesões liquenoides orais. Revisão sistemática e estudo prospectivo." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/138945.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
O conceito de reação liquenóide ou interface liquenóide foi introduzido na dermatologia para definir diversas doenças inflamatórias da pele que apresentam características histopatológicas similares. Assim como a pele, a mucosa oral é afetada por uma variedade de lesões liquenóides orais (LLOs). As LLOs foram classificadas em: lesão liquenóide de contato; lesão liquenóide a medicamento; doença do enxerto-versus-hospedeiro e lesões liquenóides não classificáveis de aspecto liquen plano-like, como a estomatite ulcerativa crônica (EUC) recentemente descrita. Além da sobreposição de características entres as lesões que compõem este grupo, o líquen plano oral (LPO) representa o principal diagnóstico diferencial. Tradicionalmente, o diagnóstico das LLOs e do LPO depende da associação clínica e histopatológica, mas em vários casos, esta abordagem não oferece um diagnóstico confiável. As realizações de estudos clínicos e laboratoriais podem auxiliar no entendimento da etiopatogenia destas doenças e refinar a capacidade de diferenciar as LLOs. Diante disto, os objetivos específicos deste estudo foram: (1) Realizar uma revisão sistemática sobre EUC com ênfase específica nas características clínicas, histopatológicas e imunopatológicas desta condição recentemente descrita; e (2) Realizar um estudo prospectivo para avaliar as características clínicas e histopatológicas das LLOs.
The concept of lichenoid reaction or lichenoid interface was introduced in dermatology to define various inflammatory diseases of the skin that have similar histopathological features. Just as the skin and oral mucosa is affected by a variety of oral lichenoid lesions (OLLs). OLLs were classified as contact Lichenoides injury; drug Lichenoides injury; chronic graft versus host and not classifiable lichenoid lesions aspect lichen planus-like, such as chronic ulcerative stomatitis (CUS) recently described. Besides the overlapping features among injuries that make up this group, oral lichen planus (OLP) is the main differential diagnosis. Traditionally, the diagnosis of OLLs and the OLP depends on the clinical and histopathological association, but in many cases, this approach does not provide a confident diagnosis. The achievement of clinical and laboratory studies may help to understand the pathogenesis of these diseases and refine the ability to differentiate OLLs. Thus, the specific objectives of this study were: (1) conduct a systematic review of CUS with particular emphasis on clinical, histopathological and immunopathological of this newly described condition; and (2) Conducting a prospective study to evaluate the clinical and histopathological characteristics of OLLs.
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Sá, Odara Maria de Sousa [UNIFESP]. "Efeitos da glicina na mucosite oral induzida por 5-fluorouracil em hamster." Universidade Federal de São Paulo (UNIFESP), 2010. http://repositorio.unifesp.br/handle/11600/9397.
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Previous issue date: 2010-07-28Mucosite oral é complicação comum no tratamento do câncer. A glicina demonstra efeito antiinflamatório, imunomodulador e citoprotetor. Este estudo tem como objetivo avaliar os efeitos da suplementação de glicina na reparação da mucosite oral induzida por 5-fluorouracil em hamster. Os animais foram divididos em dois grupos: grupo experimental (GI; n=20) e grupo controle (GII; n=20) ambos receberam injeção intraperitoneal de 5-fluorouracil no 1° e 3° dia. Os animais tiveram a sua bolsa jugal direita evertida e arranhada superficialmente no dia 3. O Grupo I, foi submetido ao tratamento com glicina a 5% por infusão intraperitoneal durante 7 dias e o Grupo II, recebeu placebo. A mucosa do GI e GII foi avaliada clinicamente, por meio de escore, no D3 e D7. Ao final do experimento a bolsa jugal dos animais de ambos os grupos foi retirada e avaliada segundo parâmetros histopatológicos e bioquímicos. Os grupos I e II apresentaram acentuado processo inflamatório durante o período inicial, segundo a avaliação clínica. No GI houve redução da severidade da mucosite, diminuição do processo inflamatório, cicatrização acelerada e redução da peroxidação lipídica quando comparado ao GII no final do experimento (p < 0,001). A suplementação com glicina demonstrou ser promissor instrumento para tratamento da mucosite, devido aos seus efeitos no processo inflamatório. Palavras chaves: Glicina; Estomatite; Fluoruracila; Cricetulus.
Oral mucositis is common complication in cancer treatment. Glycine shows a anti-inflammatory, immunomodulatory and cytoprotective. This study aims to evaluate the effects of supplementation of glycine in the repair of oral mucositis induced by 5-fluorouracil in hamsters. The animals were divided into two groups: experimental group (GI, n = 20) and control group (GII, n = 20) both received intraperitoneal injection of 5-fluorouracil in the days 1 and 3. The animals had their right pouch everted and scratched the surface on day 3. Group I was treated with glycine 5% by intraperitoneal infusion for 7 days and Group II, not supplemented. The mucosa of the GI and GII was evaluated clinically, by scoring in D3 and D7. At the end , the cheek pouch of animals from both groups was removed and evaluated by histopathological parameters and biochemical . Groups I and II showed marked inflammatory process during the initial period, according to clinical evaluation. In GI decreased the severity of mucositis, reduction of inflammation, accelerated healing and decreased lipid peroxidation when compared to GII at the end of the experiment. Supplementation with glycine proves to be a promising tool for treatment of mucositis due to its effectson the inflammatory process.
TEDE
BV UNIFESP: Teses e dissertações
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Ferrisse, Túlio Morandin. "Análise das características clínica, histopatológica e imunopatológica das lesões liquenoides orais. Revisão sistemática e estudo prospectivo /." Araraquara, 2016. http://hdl.handle.net/11449/138945.
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Orientador: Andreia BufalinoResumo: O conceito de reação liquenóide ou interface liquenóide foi introduzido na dermatologia para definir diversas doenças inflamatórias da pele que apresentam características histopatológicas similares. Assim como a pele, a mucosa oral é afetada por uma variedade de lesões liquenóides orais (LLOs). As LLOs foram classificadas em: lesão liquenóide de contato; lesão liquenóide a medicamento; doença do enxerto-versus-hospedeiro e lesões liquenóides não classificáveis de aspecto liquen plano-like, como a estomatite ulcerativa crônica (EUC) recentemente descrita. Além da sobreposição de características entres as lesões que compõem este grupo, o líquen plano oral (LPO) representa o principal diagnóstico diferencial. Tradicionalmente, o diagnóstico das LLOs e do LPO depende da associação clínica e histopatológica, mas em vários casos, esta abordagem não oferece um diagnóstico confiável. As realizações de estudos clínicos e laboratoriais podem auxiliar no entendimento da etiopatogenia destas doenças e refinar a capacidade de diferenciar as LLOs. Diante disto, os objetivos específicos deste estudo foram: (1) Realizar uma revisão sistemática sobre EUC com ênfase específica nas características clínicas, histopatológicas e imunopatológicas desta condição recentemente descrita; e (2) Realizar um estudo prospectivo para avaliar as características clínicas e histopatológicas das LLOs.
Abstract: The concept of lichenoid reaction or lichenoid interface was introduced indermatology to define various inflammatory diseases of the skin that have similarhistopathological features. Just as the skin and oral mucosa is affected by a variety oforal lichenoid lesions (OLLs). OLLs were classified as contact Lichenoides injury;drug Lichenoides injury; chronic graft versus host and not classifiable lichenoidlesions aspect lichen planus-like, such as chronic ulcerative stomatitis (CUS) recentlydescribed. Besides the overlapping features among injuries that make up this group,oral lichen planus (OLP) is the main differential diagnosis. Traditionally, the diagnosisof OLLs and the OLP depends on the clinical and histopathological association, butin many cases, this approach does not provide a confident diagnosis. Theachievement of clinical and laboratory studies may help to understand thepathogenesis of these diseases and refine the ability to differentiate OLLs. Thus, thespecific objectives of this study were: (1) conduct a systematic review of CUS withparticular emphasis on clinical, histopathological and immunopathological of thisnewly described condition; and (2) Conducting a prospective study to evaluate theclinical and histopathological characteristics of OLLs.
Mestre
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Kernmaier, Alice Maria. "Untersuchung der lokalen Viruslast bei der felinen Gingivo-Stomatitis nach der Kombinationstherapie mit felinem rekombinantem Omega-Interferon." Doctoral thesis, Universitätsbibliothek Leipzig, 2007. http://nbn-resolving.de/urn:nbn:de:bsz:15-20071015-142334-3.
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Aus dem Patientengut einer Fachklinik für Klein- und Heimtiere wurden 11 nicht vorbehandelte Katzen zwischen einem und zwölf Jahren mit mittel- bis hochgradiger Gingivo-Stomatitis ausgewählt. Diese wurden für zwölf Wochen (84 Tage) stationär aufgenommen und nach einem standardisierten Therapiekonzept behandelt: Am ersten Tag erfolgte nach dentalem Röntgen eine umfassende Zahnsanierung. An den Tagen 0, 14, 28, 42 und 84 wurde Interferon (Virbagen Omega® des Herstellers Virbac S.A.®, Carros Cedex, Frankreich) unter Sedation lokal, d.h. submukosal mit 1 ME/kg KGW injiziert. An den Tagen 56, 58, 60 und 62 erfolgte die Interferongabe systemisch. Begleittherapien wurde nach Bedarf eingesetzt, jedoch ohne die Verwendung von Glukokortikoiden und Hormonpräparaten. Verfüttert wurde ausschließlich Futter des Herstellers Royal Canin®, Köln, in den ersten 14 Tagen das Feuchtfutter Royal Canin convalescence support®, ab Tag 15 Royal Canin intestinal® Feucht- und Trockenfutter. An allen Behandlungstagen wurden zur qualitativen Virusbestimmung Tupferproben der am stärksten entzündeten Bezirke entnommen, die Maulhöhle nach einem festen System abfotografiert und die Veränderungen in Formblättern (Stärke der Faucites, Gingivitis, Buccostomatitis, Größe der Fläche und Art der Veränderung) und Grafik-charts festgehalten. Am ersten und letzten Tag wurden außerdem Biopsien zur quantitativen Bestimmung der Viruslast entnommen. Die Entwicklungen in folgenden Bereichen wurden anhand fixer Kriterien 14-tägig festgehalten: Allgemeinzustand, Schmerzen bei der Maulöffnung, Halitosis/zäher Speichel, Größe der Mandibularlymphknoten, Appetit, Schmerzen bei Futter-aufnahme oder Gähnen, Hypersalivation, Aktivität, Putztrieb und Zugänglichkeit. Die klinischen Verbesserungen waren bei allen Tieren schon nach 14 Tagen augenfällig. Der Hauptvorstellungsgrund der Besitzer, Appetitlosigkeit und Schmerzen bei der Futteraufnahme waren einer fast ungestörten Futteraufnahme gewichen, diese konnte in den folgenden Wochen kaum noch optimiert werden. Die entzündlichen Ulzerationen und Proliferationen der Maulhöhle halbierten sich innerhalb der ersten 14 Tage, nach 84 Tagen war der Heilungsprozess bei acht der elf Katzen abgeschlossen. Die persistierenden Proliferationen der restlichen Katzen waren allerdings nicht entzündlich und beeinflussten die Futteraufnahme nicht. Allgemeinzustand, Aktivität, Putztrieb und Zugänglichkeit stiegen bei zehn von elf Katzen bis zum 42. Tag etwa linear auf artspezifisches Normalniveau an und blieben hier konstant. Hypersalivation und Schwellung der Mandibularlymphknoten legte sich, so vorhanden, bei allen Tieren bis auf zwei innerhalb von 28 Tagen, bei diesen beiden war bis zum 84. Tag nur eine geringgradige Verbesserung zu beobachten. Nach der systemischen Vier-Tages-Therapie wurde ein erneutes Aufflackern der Gingivo-Stomatitis etwa auf das Niveau des 56. Tages beobachtet, allerdings ohne Folgen für die Verhaltensparameter. Eine Reduktion der Viruslast konnte trotz der eindrucksvollen Verbesserungen im klinischen Bereich in keinem Fall festgestellt werden. Die FIV/FeLV-positiven Katzen sprachen langfristig wesentlich schlechter auf das Therapiekonzept an als die übrigen Probanden. Daher gilt es bei diesen Tieren vor Interferoneinsatz kritisch zwischen den nicht unerheblichen Kosten und der zweifelhaften Prognose abzuwägen. Generell kann das Therapiekonzept Zahnsanierung – Interferon – Begleittherapie nach erfolgtem FIV/FeLV-Test bei der felinen Gingivo-Stomatitis als klinisch erfolgreich betrachtet werden.
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Lee, Amy. "Ribosome-Mediated Specificity in Vesicular Stomatitis Virus mRNA Translation Defines a New Role for rpL40 during Initiation." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10164.
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Vesicular stomatitis virus (VSV) infection causes inhibition of host protein synthesis, in part by sequestering initiation factors required for mRNA cap recognition. The viral mRNAs share a common mRNA structure to those of the host cell, with a 5' cap and 3' polyadenylate tail, but continue to be efficiently translated despite host translational shutoff. This observation suggests that a non-canonical translation pathway is utilized for viral protein synthesis. To investigate this pathway, we performed an RNA interference screen to identify genes required for VSV replication. In contrast to bulk cellular translation, viral translation is hypersensitive to knockdown of a protein constituent of the 60S ribosomal subunit, rpL40. Depletion of rpL40 diminishes VSV protein synthesis by >90% and is restored through complementation with an siRNA-resistant mutant of rpL40. To delineate the mechanism by which rpL40 is required for viral protein synthesis, we reconstituted translation of VSV mRNA in yeast extracts in vitro. In the absence of rpL40, we show that the two ribosomal subunits fail to associate on VSV mRNA, and the small subunit does not scan to the initiation codon. Regulation by rpL40 occurs in context of the large subunit, providing direct evidence for translational control by the ribosome itself. This rpL40- dependent mechanism of translation initiation is broadly conserved within eukaryotes, governed solely through an RNA determinant, and is utilized by several viruses within the order Mononegavirales. To determine whether a subset of cellular transcripts also require rpL40 for translation, we identified polysome-associated mRNAs in yeast by deep sequencing. We demonstrate that in vitro and in vivo translation of candidate mRNAs, including factors involved in stress responses, are inhibited in the absence of rpL40. This finding suggests that rpL40 plays a critical role in transcript-specific translation during cellular stress. Collectively, our work identifies an alternative translation pathway that is specifically dependent on rpL40, revealing a previously unappreciated mechanism of protein synthesis regulation by the ribosome.
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Adatia, Femina. "A role for type I interferons in the resistance of chronic lymphocytic leukemia to vesicular stomatitis virus." Thesis, University of Ottawa (Canada), 2005. http://hdl.handle.net/10393/26833.
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Vesicular Stomatitis Virus (VSV) is a naturally oncolytic agent that is exquisitely sensitive to the antiviral effects of Type I Interferons (IFN). Although 81% of human cancer cell lines in the NCI-60 panel are sensitive to VSV, Chronic Lymphocytic Leukemia (CLL), the most common adult leukemia in the West, is resistant. It is hypothesized that this resistance is due to an intact IFN pathway in CLL cells. A quantitative PCR based approach was taken to examine the IFN gene expression profile of CLL cells. It was discovered that CLL cells constitutively express IFNbeta and IFNalphaR1 transcripts which may contribute to an inherent antiviral state in these cells. However, upon activation of CLL cells, IFN transcript levels decrease and susceptibility to VSV infection is increased. As complete sensitivity to VSV is not achieved, the contribution of other antiviral mechanisms cannot be excluded. Understanding the mechanisms of viral resistance in CLL and uncovering novel approaches of manipulating them will have future implications for VSV therapy of CLL.
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Rolim, Veronica Machado. "Caracterização clínico, patológica, imuno-histoquímica e molecular da gengivoestomatite linfoplasmocítica felina." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/94757.
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A gengivoestomatite linfoplasmocítica felina (GELF) é uma síndrome clínica frequentemente observada na medicina de felinos. Trata-se de uma síndrome provavelmente multifatorial que envolve o sistema imune dos animais, agentes infecciosos e não infecciosos, de modo que a etiologia ainda não foi totalmente determinada. Diversos agentes infecciosos como o calicivírus felino (FCV), herpesvírus felino (FHV), vírus da leucemia felina (FeLV) e vírus da imunodeficiência felina (FIV) têm sido investigados. Este trabalho apresenta uma caracterização clínico, patológica, imuno-histoquímica e molecular de 27 felinos com GELF. Quanto à raça, 85,2% eram sem raça definida, 7,4% Siamês e 7,4% Maine Coon. Os machos corresponderam a 67% dos casos e fêmeas 33%. Quanto à idade, 10% apresentavam entre 1 e 3 anos, 20% entre 4 e 6 anos, 30% entre 7 e 9 anos, 15% entre 10 e 12 anos e 25% tinham mais do que 13 anos. A idade média dos felinos foi de 8,8 anos. Os principais sinais clínicos observados foram: disfagia (83,3%), halitose (72,2%), sialorreia (44,4%), emagrecimento (38,8%), intenso desconforto oral (33,3%), sangramento oral (22,2%), pelos opacos e quebradiços (11,1%) e intensa dispneia (5,5%). Macroscopicamente, eram lesões bilaterais no arco palatino e que se estendiam até a face lateral da base da língua. Em 77,8% elas eram difusas, de aspecto proliferativo, coloração vermelho intenso, friável e que sangravam facilmente durante a manipulação e em 22,2% as lesões eram multifocais a coalescentes, por vezes formando múltiplas vesículas, no arco palatino de aspecto avermelhado e edematoso. Microscopicamente, 14,8% apresentaram uma inflamação moderada (grau 2) e 85,2% dos animais apresentaram uma inflamação grave (grau 3). Os antígenos virais do FeLV foram identificados através da técnica de IHQ no epitélio e células do infiltrado inflamatório de 29,6% dos animais que apresentavam a GELF. Os antígenos virais do FIV foram identificados nas células do infiltrado inflamatório em 3,7% dos casos. Os antígenos virais do FCV não foram identificados nas lesões de GELF. Dezoito por cento dos animais foram positivos para FIV e 37% foram positivos para FeLV no exame de PCR.Feline chronic gingivostomatitis (FCGS) is a clinical syndrome frequently observed in feline medicine. This is probably a multifactorial syndrome which involves the immune system of animals, infectious and noninfectious agent, though the causes have not been fully determined. Several infectious agents such as feline calicivirus (FCV), feline herpesvirus (FHV), feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) have been investigated. To determine clinical, pathological, immunohistochemical (IHC) and molecular characteristics of 27 cats with FCGS was developed. Regarding race, 85.2% were mixed breed, 7.4% Siamese and 7.4% Maine Coon. Males were 67% of cases and females 33%. Regarding age, 10% were between 1 and 3 years, 20% between 4 and 6 years, 30% between 7 and 9 years old, 15% between 10 and 12 years and 25% were older than 13 years. The average age of the cats was of 8.8 years. The main clinical signs were: dysphagia (83.3%), halitosis (72.2%), sialorrhoea (44.4%), weight loss (38.8%), intense oral discomfort (33.3%), oral bleeding (22.2%), hair opaque and brittle (11.1%) and severe dyspnea (5.5%). Grossly, the lesions were bilateral in the palatal arch and extending to the lateral side of the base of the tongue. In 77.7% they were diffuse, proliferative, intense red, crispy and bled easily during handling and in 22.3% lesions were multifocal to coalescing, sometimes forming multiple vesicles, the palatal arch reddish and edematous appearance. Microscopically, 14.8% showed moderate inflammation (grade 2) and 85.2% of the animals showed severe inflammation (grade 3). FeLV viral antigens were identified by IHC reaction in the epithelium and inflammatory cells of 29.6% of the animals showing FCGS. FIV viral antigens were identified in inflammatory cells in 3.7% of cases. FCV viral antigens were not identified in lesions of FCGS. Eighteen per cent of animals were positive for FIV and 37% were positive for FeLV in the PCR.
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Elsby, Rachel Jane. "The Alpha Subunit of Eukaryotic Initiation Factor 2B Is Requisite for EIF2-Mediated Transitional Suppression of Vesicular Stomatitis Virus." Scholarly Repository, 2008. http://scholarlyrepository.miami.edu/oa_dissertations/33.
Full textAbstract:
Eukaryotic initiation factor 2B (eIF2B) is a heteropentameric guanine nucleotide exchange factor (GEF) that converts inactive eIF2 GDP-bound binary complexes into active eIF2 GTP-bound complexes that can bind initiator t-RNA molecules and ribosomes to begin translation. eIF2B is functionally divided into two subcomplexes: the catalytic core comprised of eIF2B epsilon and eIF2B gamma, and the regulatory core comprised of eIF2B alpha, eIF2B beta and eIF2B delta. While the catalytic subunits are responsible for exerting GEF activity, the regulatory subunits recognize eIF2 and respond to eIF2 alpha phosphorylation. Cellular stress, such as virus infection, inhibits host protein synthesis by activating specific kinases that are capable of phosphorylating the alpha subunit of eIF2, which can then sequester eIF2B to stall guanine nucleotide exchange by a currently unresolved mechanism. Importantly, we demonstrate that loss of eIF2B alpha or expression of a variant of the human eIF2B alpha subunit harboring a single point mutation (T41A) is sufficient to neutralize the consequences of eIF2 alpha phosphorylation, and render primary MEFs significantly more susceptible to vesicular stomatitis virus infection. To extend this analysis, we further exhibit the vital function of eIF2B alpha in protein synthesis through phenotypic studies in yeast. Here, we report that this subunit can sufficiently substitute for its yeast counterpart, GCN3, and reproduce similar growth phenotypes under normal and amino acid deprived conditions. In addition, the human eIF2B alpha-T41A variant was unable derepress GCN4 translation in response to an inhibitor of amino acid biosynthesis in yeast, an activity that requires sensitivity to phosphorylation of the yeast eIF2 alpha homolog, SUI2. Previously, we have demonstrated that vesicular stomatitis virus can infect and replicate to high levels in tumor cells. Moreover, these cells appear to contain defects in eIF2 alpha-mediated translational control, plausibly due to disregulation of eIF2B activity, which overcomes the inhibitory effects of eIF2 alpha phosphorylation. Our data suggest a role for eIF2B, specifically eIF2B alpha, in suppression of translation following virus infection, and imply that this complex may contribute to oncogenic transformation. These results emphasize the importance of eIF2B alpha in mediating eIF2 kinase translation inhibitory activity and may provide insight into the complex nature of viral oncolysis and cellular transformation.
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Kamphuis, Elisabeth. "Type I interferon stimulation of lymphocytes." Giessen : VVB Laufersweiler, 2007. http://geb.uni-giessen.de/geb/volltexte/2007/4791/index.html.
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