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Journal articles on the topic 'Stool separation'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

Sukas, Sertan, Bieke Van Dorst, Agata Kryj, Ole Lagatie, Wim De Malsche, and Lieven J. Stuyver. "Development of a Lab-on-a-Disk Platform with Digital Imaging for Identification and Counting of Parasite Eggs in Human and Animal Stool." Micromachines 10, no. 12 (2019): 852. http://dx.doi.org/10.3390/mi10120852.

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We present a lab-on-a-disk technology for fast identification and quantification of parasite eggs in stool. We introduce a separation and packing method of eggs contained in 1 g of stool, allowing for removal of commonly present solid particles, fat droplets and air bubbles. The separation is based on a combined gravitational and centrifugal flotation, with the eggs guided to a packed monolayer, enabling quantitation and identification of subtypes of the eggs present in a single field of view (FOV). The prototype was tested with stool samples from pigs and humans infected with intestinal paras

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Weigum, Shannon E., Lichen Xiang, Erica Osta, Linying Li, and Gabriel P. López. "Hollow silica microspheres for buoyancy-assisted separation of infectious pathogens from stool." Journal of Chromatography A 1466 (September 2016): 29–36. http://dx.doi.org/10.1016/j.chroma.2016.09.002.

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Torres, J., C. Palmela, H. Brito, et al. "The gut microbiota, bile acids and their correlation in primary sclerosing cholangitis associated with inflammatory bowel disease." United European Gastroenterology Journal 6, no. 1 (2017): 112–22. http://dx.doi.org/10.1177/2050640617708953.

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Background Patients with primary sclerosing cholangitis associated with inflammatory bowel disease (PSC-IBD) have a very high risk of developing colorectal neoplasia. Alterations in the gut microbiota and/or gut bile acids could account for the increase in this risk. However, no studies have yet investigated the net result of cholestasis and a potentially altered bile acid pool interacting with a dysbiotic gut flora in the inflamed colon of PSC-IBD. Aim The aim of this study was to compare the gut microbiota and stool bile acid profiles, as well as and their correlation in patients with PSC-IB

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Lindo, J. F., R. D. Robinson, S. I. Terry, et al. "Age-prevalence and household clustering ofStrongyloides stercoralisinfection in Jamaica." Parasitology 110, no. 1 (1995): 97–102. http://dx.doi.org/10.1017/s0031182000081099.

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The epidemiology ofStrongyloides stercoraliswas studied in families of clinical (reference) cases and their neighbours at endemic foci in Jamaica. Thirteen foci were studied based on the place of residence of a reference case. For each household of a reference case, the 4 most proximal neighbourhood households (spatial controls) were included in the study. Out of 312 persons contacted 244 were followed up using questionnaires, stool examination and serology. Prevalence of infection based on stool examination was 3·5% and on ELISA 24·2%. Prevalence increased with age but was not related to gend

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Gong, Jun, Nazli Dizman, Valeriy Poroyko, et al. "Gut microbiome composition and response to sunitinib in metastatic renal cell carcinoma (mRCC)." Journal of Clinical Oncology 36, no. 6_suppl (2018): 657. http://dx.doi.org/10.1200/jco.2018.36.6_suppl.657.

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657 Background: Recent evidence supports a link between stool microbiome composition and immunotherapy response. However, it is unclear how the microbiome may influence clinical outcome in mRCC where vascular endothelial growth factor-targeted therapies remains a standard of care. Methods: Five consecutive stool samples were collected at baseline and at weeks 2, 3, 4 and 12 of treatment with sunitinib in patients (pts) with mRCC. In responders (R: complete/partial response and stable disease) and non-responders (P: primary progression), gut microbiota composition was assessed through extractio

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Enroth, H., and L. Engstrand. "Immunomagnetic separation and PCR for detection of Helicobacter pylori in water and stool specimens." Journal of clinical microbiology 33, no. 8 (1995): 2162–65. http://dx.doi.org/10.1128/jcm.33.8.2162-2165.1995.

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Bennet, S. M., G. De Palma, P. Bercik, A. E. Lomax, S. Vanner, and D. E. Reed. "A11 MICROBIAL AND METABOLOMIC PROFILES IDENTIFY SUBSETS OF DIARRHEA PREDOMINANT IRRITABLE BOWEL SYNDROME." Journal of the Canadian Association of Gastroenterology 3, Supplement_1 (2020): 13–15. http://dx.doi.org/10.1093/jcag/gwz047.010.

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Abstract Background Irritable bowel syndrome (IBS) patients are subtyped by predominant bowel habit rather than pathophysiological mechanisms and this may underlie challenges in identifying more effective targets for designing new treatments. Metabolomics and microbial analysis can distinguish IBS patients from healthy controls but it is unknown if they can identify pathophysiological IBS subgroups. Aims To phenotype subgroups of IBS patients using metabolomics and microbial analysis and determine if these subgroups have different underlying pain signalling mechanisms. Methods Symptom history,

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TSAI, TSUNG-YU, WAN-JU LEE, YU-JU HUANG, KUANG-LO CHEN, and TZU-MING PAN. "Detection of Viable Enterohemorrhagic Escherichia coli O157 Using the Combination of Immunomagnetic Separation with the Reverse Transcription Multiplex TaqMan PCR System in Food and Stool Samples." Journal of Food Protection 69, no. 10 (2006): 2320–28. http://dx.doi.org/10.4315/0362-028x-69.10.2320.

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Enterohemorrhagic Escherichia coli O157:H7 is an infectious pathogen and outbreaks have been reported all over the world, specifically in Australia, Canada, Japan, the United States, and in various countries in Europe and South Africa. Therefore, it is necessary to develop rapid methods to determine the target pathogens for food sanitation and disease. Three combinations of primers and probes were designed to detect and identify E. coli O157 using the TaqMan detection system which focuses on the specific genes eae, rfbO157, and stxII. Reverse transcription (RT) multiplex TaqMan PCR was carried

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Casswall, Thomas H., Hans‐Olof Nilsson, Mats Bergström, et al. "Evaluation of Serology, ¹³C‐Urea Breath Test, and Polymerase Chain Reaction of Stool Samples to Detect Helicobacter pylori in Bangladeshi Children." Journal of Pediatric Gastroenterology and Nutrition 28, no. 1 (1999): 31–36. http://dx.doi.org/10.1002/j.1536-4801.1999.tb02000.x.

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ABSTRACTBackground:Serologic methods to detect Helicobacter pylori in infants, especially in developing countries, may be limited because of decreased immune response caused by malnutrition. The true prevalence may therefore be underestimated in this age group. Urea breath test is considered to be a good screening method in children but is expensive and therefore is not suitable for screening in developing countries. Simple, inexpensive, and accurate noninvasive methods to detect H. pylori in infants and young children are needed.Methods:Enzyme immunoassay (EIA) and immunoblot (IB) serologic a

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Panasevich, Matt, Nolan Frantz, and Greg Reinhart. "PSVI-25 Inclusion of Lemna as a plant-based protein ingredient in dog and cat diets." Journal of Animal Science 98, Supplement_4 (2020): 317. http://dx.doi.org/10.1093/jas/skaa278.565.

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Abstract Our objective was to evaluate the inclusion of a novel plant-based protein (Lemna; MC Select; Parabel®; Vero Beach, FL) in dog diets at 0, 5, and 10% and cat diets at 0, 10 and 15% for palatability, stool quality, and nutrient digestibility. We hypothesized that Lemna would be a viable protein source in both cat and dog diets by showing no detriments to nutrition outcomes. All feeding tests were conducted at an independent research facility (Susquehanna, PA). A standard 2 bowl palatability test over a 2-day period was done with adult dogs and cats (n = 30 each) to determine intake rat

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NAKAYAMA, Hiroshi, Yoshiko KAWABATA, Kunihiro ISHIBASHI, and Kazumi HORIKAWA. "Evaluation of Enrichment Cultures for Detection of Escherichia coli 0157 from Human Stool Specimens by Using Immunomagnetic Separation." Journal of the Japanese Association for Infectious Diseases 74, no. 6 (2000): 527–35. http://dx.doi.org/10.11150/kansenshogakuzasshi1970.74.527.

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Fang, Haiming, Lian Fu, and Jiajia Wang. "Protocol for Fecal Microbiota Transplantation in Inflammatory Bowel Disease: A Systematic Review and Meta-Analysis." BioMed Research International 2018 (September 13, 2018): 1–11. http://dx.doi.org/10.1155/2018/8941340.

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Background. Fecal microbiota transplantation (FMT) is an emerging treatment approach for inflammatory bowel disease (IBD). The donor selection, the separation of fecal bacteria, the frequency of FMT, the way of infusion, the long-term safety, and efficacy are still uncertain. Aim. To further study the efficacy and safety and protocol of FMT for IBD. Methods. A systematic review and meta-analysis were conducted until February, 2018. Clinical remission was established as the primary outcome. Results. A total of 596 paediatric and adult IBD patients were enrolled, and 459 patients received FMT th

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Kellnerová, Sára, Silke Huber, Mariam Massri, et al. "Enzymatic Cleavage of Stx2a in the Gut and Identification of Pancreatic Elastase and Trypsin as Possible Main Cleavers." Microorganisms 11, no. 10 (2023): 2487. http://dx.doi.org/10.3390/microorganisms11102487.

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Shiga toxins (Stxs), especially the Stx2a subtype, are the major virulence factors involved in enterohemorrhagic Escherichia coli (EHEC)-associated hemolytic uremic syndrome (eHUS), a life-threatening disease causing acute kidney injury, especially in children. After oral transmission and colonization in the gut, EHEC release Stx. Intracellular cleavage of the Stx A subunit, when followed by reduction, boosts the enzymatic activity that causes damage to targeted cells. This cleavage was assumed to be mostly mediated by furin during Stx intracellular trafficking. To investigate whether this cle

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LEE, HEE-MIN, JOSEPH KWON, JONG-SOON CHOI, et al. "Rapid Detection of Norovirus from Fresh Lettuce Using Immunomagnetic Separation and a Quantum Dots Assay." Journal of Food Protection 76, no. 4 (2013): 707–11. http://dx.doi.org/10.4315/0362-028x.jfp-12-343.

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Current molecular methods that include PCR have been used to detect norovirus in many food samples. However, the protocols require removing PCR inhibitors and incorporate time-consuming concentration steps to separate virus from analyte for rapid and sensitive detection of norovirus. We developed an immunomagnetic separation (IMS) and a quantum dots (QDs) assay to detect norovirus eluted from fresh lettuce with Tris buffer containing 1% beef extract (pH 9.5). IMS facilitated viral precipitation with a 10-min incubation, whereas virus concentration using polyethylene glycol (PEG) requires more

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Braunschweig, Holger, та Thomas Kramer. "Crystal structure of μ-1κC:2(η2)-carbonyl-carbonyl-1κC-chlorido-2κCl-μ-chloridoborylene-1:2κ2B:B-[1(η5)-pentamethylcyclopentadienyl](tricyclohexylphosphane-2κP)iron(II)platinum(II) benzene monosolvate". Acta Crystallographica Section E Structure Reports Online 70, № 11 (2014): 421–23. http://dx.doi.org/10.1107/s1600536814023381.

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In the molecular structure of the dinuclear title compound [η5-(C5(CH3)5)(CO)Fe{(μ-BCl)(μ-CO)}PtCl(P(C6H11)3)]·C6H6, the two metal atoms, iron(II) and platinum(II), are bridged by one carbonyl (μ-CO) and one chloridoborylene ligand (μ-BCl). The PtIIatom is additionally bound to a chloride ligand situatedtransto the bridging borylene, and a tricyclohexylphosphane ligand (PCy3)transto the carbonyl ligand, forming a distorted square-planar structural motif at the PtIIatom. The FeIIatom is bound to a pentamethylcyclopentadienyl ligand [η5-C5(CH3)5] and one carbonyl ligand (CO), forming a piano-sto

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Dr., Muhammad Hassan Tahir Dr. Laraib Dr. Muhammad Hassaan Anwar. "PREVALANCE OF CLOSTRIDIA IN THE PATIENTS OF ACUTE DIARRHEA." Indo American Journal of Pharmaceutical Sciences 05, no. 06 (2018): 5623–27. https://doi.org/10.5281/zenodo.1297618.

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Objective: Our surroundings are fully concentrated by a group of gram positive spore forming bacteria which usually live without oxygen and found in paths of stomach and intestines. These bacteria are the cause of many diseases of intestine disorder such as pain and swelling of colon which is a part of large intestine, poisoning and inflammation of the intestines which results into diarrhea. The main purpose of this research is to identify these different types of spore forming bacteria in the patients of the hospital and match them with the controls who are healthy people. Methodology:<em> Th

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Maia, Manuel C., Valeriy Poroyko, Haejung Won, et al. "Association of microbiome and plasma cytokine dynamics to nivolumab response in metastatic renal cell carcinoma (mRCC)." Journal of Clinical Oncology 36, no. 6_suppl (2018): 656. http://dx.doi.org/10.1200/jco.2018.36.6_suppl.656.

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656 Background: Checkpoint inhibitors (CPI) represent a standard of care in mRCC. The relationship of the microbiome to benefit from CPIs has been suggested in clinical studies in melanoma (Wargo et al ASCO 2017). Here we provide updated evidence of association between microbiome composition, plasma cytokines and response to nivolumab in mRCC patients (pts). Methods: Stool and blood samples were prospectively collected from pts with mRCC at 3 time points relative to nivolumab treatment (baseline, week 4 and week 12) and used to assess gut microbiota composition in two different groups: respond

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., Md Monirul Islam, Md Golam Kabir ., Pricila Khan Moly ., A. F. M. Tariqul Islam ., and Md Abdur Rakib . "Detection of Vibrio cholerae O1 and O139 Serogroups Directly from Stool Specimens by Combined Immunomagnetic Separation and Polymerase Chain Reaction." Pakistan Journal of Biological Sciences 7, no. 10 (2004): 1654–59. http://dx.doi.org/10.3923/pjbs.2004.1654.1659.

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Lakananurak, Narisorn, Elizabeth Wall, Hilary Catron, et al. "Real-World Management of High Stool Output in Patients with Short Bowel Syndrome: An International Multicenter Survey." Nutrients 15, no. 12 (2023): 2763. http://dx.doi.org/10.3390/nu15122763.

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Background: International practice guidelines for high-stool-output (HSO) management in short bowel syndrome (SBS) are available, but data on implementation are lacking. This study describes the approach used to manage HSO in SBS patients across different global regions. Methods: This is an international multicenter study evaluating medical management of HSO in SBS patients using a questionnaire survey. Thirty-three intestinal-failure centers were invited to complete the survey as one multidisciplinary team. Results: Survey response rate was 91%. Dietary recommendations varied based on anatomy

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Sila, Sara, Marko Jelić, Ivana Trivić, Arjana Tambić Andrašević, Sanja Kolaček, and Iva Hojsak. "Gut Microbiota Composition Changes following Discontinuation of Exclusive Enteral Nutrition in Children with Crohn’s Disease." Microorganisms 11, no. 2 (2023): 505. http://dx.doi.org/10.3390/microorganisms11020505.

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This study aims to determine changes in the intestinal microbiota of children with Crohn’s disease (CD) before and during exclusive enteral nutrition (EEN) and after its discontinuation. A total of 14 newly diagnosed children with CD (median age 16.0 years; 43% female) were included in this study. Patients were initially treated with EEN and were followed for one year after EEN discontinuation. Stool samples were taken at the time of diagnosis (before EEN introduction), the second day of EEN, the last day of EEN, and every two months for one year after the discontinuation of EEN. A molecular a

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El Mouzan, Mohammad, Asaad Assiri, Ahmed Al Sarkhy, et al. "Viral dysbiosis in children with new-onset celiac disease." PLOS ONE 17, no. 1 (2022): e0262108. http://dx.doi.org/10.1371/journal.pone.0262108.

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Viruses are common components of the intestinal microbiome, modulating host bacterial metabolism and interacting with the immune system, with a possible role in the pathogenesis of immune-mediated diseases such as celiac disease (CeD). The objective of this study was to characterize the virome profile in children with new-onset CeD. We used metagenomic analysis of viral DNA in mucosal and fecal samples from children with CeD and controls and performed sequencing using the Nextera XT library preparation kit. Abundance log2 fold changes were calculated using differential expression and linear di

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Jiang, Jianlin, Kerri A. Alderisio, Ajaib Singh, and Lihua Xiao. "Development of Procedures for Direct Extraction of Cryptosporidium DNA from Water Concentrates and for Relief of PCR Inhibitors." Applied and Environmental Microbiology 71, no. 3 (2005): 1135–41. http://dx.doi.org/10.1128/aem.71.3.1135-1141.2005.

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ABSTRACT Extraction of high-quality DNA is a key step in PCR detection of Cryptosporidium and other pathogens in environmental samples. Currently, Cryptosporidium oocysts in water samples have to be purified from water concentrates before DNA is extracted. This study compared the effectiveness of six DNA extraction methods (DNA extraction with the QIAamp DNA minikit after oocyst purification with immunomagnetic separation and direct DNA extraction methods using the FastDNA SPIN kit for soil, QIAamp DNA stool minikit, UltraClean soil kit, or QIAamp DNA minikit and the traditional phenol-chlorof

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Monda, Vincenzo, Marco Carotenuto, Francesco Precenzano, et al. "Neuropeptides’ Hypothalamic Regulation of Sleep Control in Children Affected by Functional Non-Retentive Fecal Incontinence." Brain Sciences 10, no. 3 (2020): 129. http://dx.doi.org/10.3390/brainsci10030129.

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Functional non-retentive fecal incontinence (FNRFI) is a common problem in pediatric age. FNRFI is defined as unintended loss of stool in a 4-year-old or older child after organic causes have been excluded. FNRFI tends to affects up to 3% of children older than 4 years, with males being affected more frequently than females. Clinically, children affected by FNRFI have normal intestinal movements and stool consistency. Literature data show that children with fecal incontinence have increased levels of separation anxiety, specific phobias, general anxiety, attention-deficit/hyperactivity disorde

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Stafford, Amanda, Jie (James) Min Lam, Amedra Basgaran, et al. "Effect of tocotrienol and tocopherol regulation by the microbiome on colitis-associated cancer." Journal of Clinical Oncology 42, no. 16_suppl (2024): e15512-e15512. http://dx.doi.org/10.1200/jco.2024.42.16_suppl.e15512.

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e15512 Background: The association between inflammatory bowel disease (IBD) and a higher risk of colorectal cancer (CRC) has been well established, and is commonly attributed to chronic inflammation and a predisposition to genomic instability ( Feagins et al., 2009 ). Independently, an altered gut microbiome has been evidenced in both IBD ( Halfvarson et al., 2017 ) and CRC ( Marchesi et al., 2011 ). It is unknown whether the altered the IBD-associated microbiome is able to directly influence carcinogenesis via an inflammation-independent mechanisms. Methods: Microbiome analysis was performed

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GIAMMANCO, G. M., F. BONURA, F. DI BERNARDO, et al. "Introduction and prolonged circulation of G12 rotaviruses in Sicily." Epidemiology and Infection 144, no. 9 (2016): 1943–50. http://dx.doi.org/10.1017/s0950268815003258.

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SUMMARYGenotype G12 strains are now considered to be the sixth most prevalent human rotaviruses worldwide. In two Sicilian cities, Palermo and Messina, surveillance of rotavirus circulation performed since 1985 and 2009, respectively, did not detect G12 strains until 2012. From 2012 to 2014 rotavirus infection was detected in 29·7% of 1647 stool samples collected from children admitted for acute gastroenteritis to three Sicilian hospitals in Palermo, Messina and Ragusa. In 2012, G12P[8] was first detected in Palermo and then in Messina where it represented the second most frequent genotype (20

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Chang, Hung-Yang, Jen-Shiu Chiang Chiau, Jui-Hsing Chang, et al. "Characteristics of Gut Microbiota in Small for Gestational Age Infants with Very Low Birth Weight." Nutrients 14, no. 23 (2022): 5158. http://dx.doi.org/10.3390/nu14235158.

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Small for gestational age (SGA) birth is associated with high rates of mortality and morbidity in preterm infants. The aim of this preliminary observational study was to investigate the difference in gut microbiota between SGA and appropriate for gestational age (AGA) preterm infants with very low birth weight (VLBW). We included 20 VLBW preterm infants (SGA, n = 10; AGA, n = 10) in this study. Stool samples were collected on days 7, 14, and 30 after birth. We performed 16S ribosomal DNA sequencing to compare microbiota composition between both groups. The SGA group exhibited a lower abundance

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Faris, Richard, and Leandro Hackenhaar. "215 Compensatory Growth Following One or Two Weeks of Lysine Restriction at Weaning." Journal of Animal Science 101, Supplement_2 (2023): 135–36. http://dx.doi.org/10.1093/jas/skad341.151.

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Abstract At weaning it is increasingly common to feed low crude protein (CP) and low standardized ileal digestible (SID) lysine (LYS) diets for improved health outcomes. This nutrient restriction often leads to decreased growth. Compensatory growth in this discussion is a period of enhanced growth that occurs after a period of nutrient restriction. This experiment was conducted to determine if compensatory growth occurs after one or two-weeks restriction of SID LYS at weaning. For the experiment, pigs (n = 252; ~23.5 days of age, 7.24 ± 1.02 kg BW, 8 reps/treatmentt, 5 or 6 pigs/pen) were rand

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Wahba, Mina, Heaven D. Chitemo, Vyacheslav R. Misko, et al. "A Modified Sample Preparation Protocol for High-Efficiency Lab-on-a-Disk-Based Separation and Single-Image Quantification of Soil-Transmitted Helminth Parasite Eggs in Stool." Micromachines 16, no. 8 (2025): 847. https://doi.org/10.3390/mi16080847.

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Soil-transmitted helminths (STHs) present a significant global health challenge, particularly in tropical and subtropical regions. The current diagnostic standard involves the microscopic examination of a stool smear but it lacks sensitivity to detect infections of low intensity. Innovative solutions like lab-on-a-disk (LoD) technologies are emerging, showing promise in detecting low-intensity infections. Field tests conducted using our SIMPAQ (single-image parasite quantification) LoD device have demonstrated its potential as a diagnostic tool, especially for such low-intensity infections. Ne

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Di Cristanziano, Veronica, Fedja Farowski, Federica Berrilli, et al. "Analysis of Human Gut Microbiota Composition Associated to the Presence of Commensal and Pathogen Microorganisms in Côte d’Ivoire." Microorganisms 9, no. 8 (2021): 1763. http://dx.doi.org/10.3390/microorganisms9081763.

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Background: The human gut microbiota is a microbial ecosystem contributing to the maintenance of host health with functions related to immune and metabolic aspects. Relations between microbiota and enteric pathogens in sub-Saharan Africa are scarcely investigated. The present study explored gut microbiota composition associated to the presence of common enteric pathogens and commensal microorganisms, e.g., Blastocystis and Entamoeba species, in children and adults from semi-urban and non-urban localities in Côte d’Ivoire. Methods: Seventy-six stool samples were analyzed for microbiota composit

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Sobhonslidsuk, Abhasnee, Suwannee Chanprasertyothin, Tanjitti Pongrujikorn, et al. "The Association of Gut Microbiota with Nonalcoholic Steatohepatitis in Thais." BioMed Research International 2018 (2018): 1–8. http://dx.doi.org/10.1155/2018/9340316.

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Objectives. Nonalcoholic steatohepatitis (NASH) can progress to advanced fibrosis; the link between intestinal bacterial overgrowth and NASH has been proposed. Gut microbiota may promote inflammation and provoke disease progression. We evaluated gut microbiota pattern in NASH and its influencing factors. Methods. A case-controlled study with sixteen NASH and eight control subjects was done. We performed DNA extraction from stool samples and bacterial 16S rRNA sequencing using MiSeq™. The sequences were clustered into operational taxonomic units using Quantitative Insights Into Microbial Ecolog

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Peredereev, Sergey S., Vyacheslav G. Svarich, Aleksandr I. Tikhomirov, Roman A. Ti, and Yulia A. Oleinikova. "Diagnosis and treatment of duodenal fistula in a child: case report." Russian Journal of Pediatric Surgery, Anesthesia and Intensive Care 14, no. 3 (2024): 413–20. http://dx.doi.org/10.17816/psaic1825.

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In children, duodenal fistulas are often formed against the background of foreign bodies of the gastrointestinal tract (nails and magnetic balls), rarely congenital fistulas. A patient aged 7 years and 9 months was treated for a duodenal fistula in surgical department no. 3 of the clinical hospital of St. Petersburg State Pediatric Medical University. The patient has had repeated vomiting and loose stools since 2023, which were interpreted as an intestinal infection. An overview X-ray of the abdominal organs revealed two X-ray contrast foreign bodies, presumably batteries. In obtaining additio

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Pogreba Brown, Kristen, Erika Austhof, Caitlyn M. McFadden, et al. "Determining the incidence, risk factors and biological drivers of irritable bowel syndrome (IBS) as part of the constellation of postacute sequelae of SARS-CoV-2 infection (PASC) outcomes in the Arizona CoVHORT-GI: a longitudinal cohort study." BMJ Open 15, no. 1 (2025): e095093. https://doi.org/10.1136/bmjopen-2024-095093.

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IntroductionPostacute sequelae of SARS-CoV-2 infection (PASC) are extensive. Also known as long COVID, primary outcomes reported are neurologic, cardiac and respiratory in nature. However, several studies have also reported an increase in gastrointestinal (GI) symptoms and syndromes following COVID-19. This study of PASC will include extensive analyses of GI symptoms, determine if people with pre-existing irritable bowel syndrome (IBS) are at higher risk of developing PASC generally or PASC-GI, and which biomarkers are impacted and to what degree. This R01 study is being funded by the National

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Tomlin, J., and N. W. Read. "The effect of resistant starch on colon function in humans." British Journal of Nutrition 64, no. 2 (1990): 589–95. http://dx.doi.org/10.1079/bjn19900058.

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Starch that is resistant to human amylases forms during the cooking and subsequent cooling of some foods, and may therefore be a substrate for the bacterial flora of the colon. It is thus possible that resistant starch (RS) will affect colon function in a similar manner to non-starch polysaccharides. To test this theory, a group of eight volunteers took two diet supplements for 1 week each in a random order with a 1 week separation. One supplement comprised mainly 350 g Cornflakes/d and the other 380 g Rice Krispies/d, providing 10.33 and 0.86 g RS/.d respectively. The amounts of amylase-diges

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Zhang, Guangming, and Andrej Weintraub. "Rapid and Sensitive Assay for Detection of Enterotoxigenic Bacteroides fragilis." Journal of Clinical Microbiology 36, no. 12 (1998): 3545–48. http://dx.doi.org/10.1128/jcm.36.12.3545-3548.1998.

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Bacteroides fragilis is an obligatory anaerobic, gram-negative bacterium found among the normal intestinal flora of humans. Enterotoxigenic strains of B. fragilis (ETBF) have been associated with diarrheal diseases in humans and animals. The enterotoxin of ETBF induces fluid changes in ligated intestinal segments and cytotoxic response in HT29/C1 cells. By using a pair of monoclonal antibodies (MAbs; MAb C3 and MAb 4H8) specific for the lipopolysaccharide of B. fragilis, an assay based on immunomagnetic separation (IMS) in combination with PCR (IMS-PCR) was developed. After DNA extraction, a 2

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Khedr, Elsayed Mohamed Elsayed, Mahmoud Tarek, Hadeer Mohamed NasrEl-Din, Ayman Hussein Abdelsattar, and Osama Abdelazim. "Laparoscopic-assisted Combined Abdominal and Sacral Approach for Sacrococcygeal Teratoma Altman II/III Excision: Case Series." Journal of Indian Association of Pediatric Surgeons 30, no. 2 (2025): 215–19. https://doi.org/10.4103/jiaps.jiaps_174_24.

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ABSTRACT Context: This study aims to optimize patient outcomes and surgical efficacy by integrating laparoscopic and sacral approaches for the excision of presacral teratomas, focusing on Altman type II–III classifications. Methods: A case series was conducted with seven patients diagnosed with sacrococcygeal teratoma (SCT) Altman type II–III. Short- and intermediate-term surgical outcomes were assessed following a combined laparoscopic and sacral approach. The diagnosis was based on clinical examination, elevated serum alpha-fetoprotein levels, and preoperative pelvic magnetic resonance imagi

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Journal, Baghdad Science. "Isolation & Pathogenic Study on Proteus mirabilis." Baghdad Science Journal 7, no. 1 (2010): 317–426. http://dx.doi.org/10.21123/bsj.7.1.317-426.

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During the period October 2003 till July 2004, about (253) urine samples have been collected from urinary tract infection. The study has shown that the bacterium Proteus mirabilis is the responsible for (11.85%) of the urinary tracts infections. Also, the study has declared that the ratio of separation this bacterium from women was (7.51%) and it is higher than the ratio of separation in both men and children which ranged (1. 58%) and (2.76%) respectively . About (30) samples of stool have been collected from children and the ratio of isolation this bacterium has been showen to be( 30%) from c

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Zintchem, Roger, Gaëtan Olivier Fankem, Joseph Ngakou Mukam, et al. "Ethopharmacological Approach, in vivo Antidiarrhoeic Properties of Aqueous Leaf Extract of Mallotus oppositifolium (Euphorbiaceae) and Isolation of Antidiarrheal Compound." European Journal of Medicinal Plants 34, no. 7 (2023): 1–16. http://dx.doi.org/10.9734/ejmp/2023/v34i71145.

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Mallotus oppositifolium. Müll. Arg (Euphorbiaceae) is commonly used in the Center Region of Cameroon against diarrheal syndromes and joint pains. In order to provide a scientific basis for its use in the traditional treatment of diarrhea, we proposed to evaluate the effects of aqueous extracts of the leaves of this plant on the characteristic patterns of diarrhea. The ethnopharmacological approach was carried out in Gouifé village in Bafia (Cameroon) by observing traditional therapeutic practices and by the interview of 5 traditional therapists and 11 of their patients about the traditional tr

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Tiele, Wicaksono, Kansara, Arasaradnam, and Covington. "Breath Analysis Using eNose and Ion Mobility Technology to Diagnose Inflammatory Bowel Disease—A Pilot Study." Biosensors 9, no. 2 (2019): 55. http://dx.doi.org/10.3390/bios9020055.

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Early diagnosis of inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), remains a clinical challenge with current tests being invasive and costly. The analysis of volatile organic compounds (VOCs) in exhaled breath and biomarkers in stool (faecal calprotectin (FCP)) show increasing potential as non-invasive diagnostic tools. The aim of this pilot study is to evaluate the efficacy of breath analysis and determine if FCP can be used as an additional non-invasive parameter to supplement breath results, for the diagnosis of IBD. Thirty-nine subjects were re

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Ostrowska, Malgorzata, Elwira Komoń-Janczara, Bozena Mikoluc, et al. "The Gut Mycobiome and Nutritional Status in Paediatric Phenylketonuria: A Cross-Sectional Pilot Study." Nutrients 17, no. 15 (2025): 2405. https://doi.org/10.3390/nu17152405.

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Background: Phenylketonuria (PKU) is a metabolic disorder managed through a strict, lifelong low-phenylalanine diet, which may influence gut microbiome dynamics. While gut bacterial alterations in PKU are increasingly investigated, the fungal community (mycobiome) remains largely unexplored. This study compared gut mycobiome composition and dietary profiles of paediatric PKU patients and healthy controls, stratified by age (<10 and 10–18 years). Methods: Stool samples from 20 children (10 PKU, 10 controls) were analysed using ITS1/ITS2 amplicon sequencing. Nutritional status was assessed us

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Hodges, P., M. Mweetwa, V. Kayamba, et al. "P1321 Inflammatory bowel disease and the rural to urban microbiome transition in Zambia." Journal of Crohn's and Colitis 19, Supplement_1 (2025): i2382—i2384. https://doi.org/10.1093/ecco-jcc/jjae190.1495.

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Abstract Background Existing evidence suggests that incidence of Inflammatory Bowel Disease (IBD) is increasing in geographical regions where it has traditionally been viewed as uncommon, including sub-Saharan Africa.(1) The nutrition transition that has occurred alongside rapid urbanisation in this region is one possible explanation for this. For instance, a shift towards a ‘Western-style’ diet may lead to subsequent changes in the intestinal microbiota resulting in a ‘pro-inflammatory’ environment. To investigate this hypothesis, we examined the faecal microbiota across several different sec

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Ellul, S., P. Rausch, A. Pisani, C. Bang, P. Ellul, and A. Franke. "P673 Microbial diversity in newly diagnosed treatment naïve Inflammatory Bowel Disease patients." Journal of Crohn's and Colitis 15, Supplement_1 (2021): S594—S595. http://dx.doi.org/10.1093/ecco-jcc/jjab076.793.

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Abstract Background The role of microbiome with the alteration between commensal and pathogenic bacteria, has been linked to IBD. Meanwhile Escherichia coli Nissle 1917, Lactobacillus rhamnosus GG (LGG) and faecal transplantation are used in IBD. The aim of this study was to prospectively determine faecal microbiota composition of newly diagnosed treatment naïve IBD patients. Methods Patients diagnosed with IBD between January 2018-September 2019 were recruited. Clinical data was collected and patients asked to submit stool samples for microbiome analysis. Stool samples from a control populati

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Sarda, Shrutii, David Merrill, Heesun Shin, et al. "680 Automated Ion torrent based solution enables accurate gut microbiome quantification of bacterial species relevant to research in cancer and its response to immunotherapy." Journal for ImmunoTherapy of Cancer 8, Suppl 3 (2020): A719. http://dx.doi.org/10.1136/jitc-2020-sitc2020.0680.

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BackgroundA low-cost targeted solution to profiling gut microbial diversity is sequencing of the 16S rRNA gene; however, it is often insufficient to gain species level resolution due to high homology across different bacteria. Therefore, we developed a first-of-its-kind targeted sequencing solution that supplements 16S gene targets, with highly species-specific primers for a cohort of 73 bacteria associated with research in diabetes, cancer and its response to immunotherapy, gastrointestinal and auto-immune disorders. This assay performs at 100% sensitivity and specificity for the species-leve

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Iftimi, Raluca Suzana, Ana Maria Alexandra Stanescu, Carmen Anton, Smaranda Diaconescu, Nicoleta Gimiga, and Oana-Maria Rosu. "Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies in Pediatric Infection with Helicobacter Pylori." Revista de Chimie 71, no. 4 (2020): 446–55. http://dx.doi.org/10.37358/rc.20.4.8087.

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The current methods for the diagnosis of Helicobacter pylori infection in children include invasive (direct) methods, that enable the detection of the bacteria or the bacterial urease in gastric biopsies, or noninvasive (indirect) methods, which consist of researching specific antibodies, antigens and urease in other different samples (serum, saliva, urine, stool, exhaled air). The urease functions in H. pylori infection is to neutralize gastric acid by producing NH3 through the hydrolysis of urea. Monochloramine, a NH3-derived compounds has cytotoxic effects on host cells. The Western Blot me

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Mavrogeorgis, Emmanouil, Sophie Valkenburg, Justyna Siwy, et al. "Integration of Urinary Peptidome and Fecal Microbiome to Explore Patient Clustering in Chronic Kidney Disease." Proteomes 12, no. 2 (2024): 11. http://dx.doi.org/10.3390/proteomes12020011.

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Millions of people worldwide currently suffer from chronic kidney disease (CKD), requiring kidney replacement therapy at the end stage. Endeavors to better understand CKD pathophysiology from an omics perspective have revealed major molecular players in several sample sources. Focusing on non-invasive sources, gut microbial communities appear to be disturbed in CKD, while numerous human urinary peptides are also dysregulated. Nevertheless, studies often focus on isolated omics techniques, thus potentially missing the complementary pathophysiological information that multidisciplinary approache

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Annakib, Soufyan, Lucia Grenga, Catherine Dunyach-Remy, et al. "Gut microbiota as a biomarker for the efficacy of pembrolizumab in patients with advanced urothelial carcinoma treated in a prospective multicenter study." Journal of Clinical Oncology 43, no. 5_suppl (2025): 825. https://doi.org/10.1200/jco.2025.43.5_suppl.825.

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825 Background: Pembrolizumab (Pb) is a treatment (trt) for advanced urothelial carcinoma (aUC). In this study, we analyzed the fecal metaproteome of aUC patients (pts) to investigate the differences in the taxonomic and functional composition of the gut microbiome in responders (R) vs non-responders (NR) to pembrolizumab monotherapy as second-line trt. Methods: We designed a prospective multicenter study in France. Stool samples were collected before and nine weeks after the start of Pb, with a maximum follow-up of 36 weeks. Trt efficacy was evaluated using the Response Evaluation Criteria in

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Almashhadany, Dhary Alewy, Hero Ismael Mohammed, and Abdulmunem Dherar Abdullah Aljoborey. "Review on Helicobacteriosis Associated Diseases." International Journal of Membrane Science and Technology 10, no. 5 (2023): 24–34. http://dx.doi.org/10.15379/ijmst.v10i5.2361.

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Helicobacteriosis is a bacterial infection caused by Helicobacter pylori (H.pylori)(HP). which was discovered in 1982 by Australian scientists Barry Marshall and Robin Warren, who were later awarded the Nobel Prize in 2005.Epidemiological evidence has shown that H.pylori colonizes the upper gastrointestinal tract(GIT), and the infection is one of the most common human bacterial pathogens worldwide, with 4.4 billion infected individuals in 2015, but the prevalence varies according to situation and hygiene principles. Since this first isolation, it has become apparent that this organism may be o

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Yu.R., Mirzaev, Khamroev T.T., Ruzimov E.M., et al. "Evaluation of the Effect on the Nervous System of Substances with an Alkaloid Structure Having Antitumor Activity." International Journal of Health & Medical Research 01, no. 05 (2022): 74–77. https://doi.org/10.5281/zenodo.7439900.

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Indole alkaloids including sedative activity of pyrazolone iodine methylate and pyrazolone chloride were caused by alcohol sleep and feeling against arousal or fear effects were studied by the Sonberg method. In this study, pyrazoline iodine methylate and pyrazaline chloride reduced sleep time by increasing the sleep time caused by alcohol, in the Sonberg method, the excretion of urine and excrement by experimental animals decreased due to noise and external factors. This indicates that the studied substances have a calming effect.

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Sokolov, L. I. "Unit for separating ingots welded to the stool." Metallurgist 30, no. 1 (1986): 21–22. http://dx.doi.org/10.1007/bf01133977.

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Prakash, Charu. "Sewage Analysis as a Tool for Environmental Surveillance of SARS-CoV-2: Experience from Delhi, India." Journal of Communicable Diseases 53, no. 02 (2021): 1–13. http://dx.doi.org/10.24321/0019.5138.202119.

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Background: The COVID-19 pandemic caused by SARS-CoV-2 has resulted in more than 150 thousand deaths in India. SARS-CoV-2 is known to be excreted in stool samples in the range of 102 to 108 gene copies per gram of faeces. Waste-water Based Epidemiology has been advocated by researchers all over the world as a method for environmental surveillance of COVID-19. Objectives: The present study was carried out to determine the presence of SARS-CoV-2, and to quantify it in sewage samples in Delhi, and to estimate the Sensitivity of WBE for COVID-19 Surveillance. Methods: A total of 49 waste water sam

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Mohammad Rahimi, Hanieh, Ehsan Javanmard, Ali Taghipour, Ali Haghighi, and Hamed Mirjalali. "Multigene typing of Giardia Duodenalis isolated from tuberculosis and non-tuberculosis subjects." PLOS ONE 18, no. 3 (2023): e0283515. http://dx.doi.org/10.1371/journal.pone.0283515.

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Giardia duodenalis is a cryptic protozoan, which has eight assemblages (A-H). Assemblages A and B are the main genotypes reported from humans with probable anthroponotic and zoonotic transmission. The current study aimed to characterize G. duodenalis assemblages in tuberculosis (TB) patients and healthy subjects using multilocus genotyping (MLG). Thirty Giardia-positive stool samples, which were obtained from TB patients and healthy subjects were included in the study. After total DNA extraction, three β-giardin (bg), triosephosphate isomerase (tpi), glutamate dehydrogenase (gdh) genes were am

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