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Journal articles on the topic 'Streptococcus uberis'

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Published: 4 June 2021

Last updated: 25 July 2025

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1

Brown, M. B., and A. E. Scasserra. "Antimicrobial resistance in streptococcal species isolated from bovine mammary glands." American Journal of Veterinary Research 51, no. 12 (1990): 2015–18. http://dx.doi.org/10.2460/ajvr.1990.51.12.2015.

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SUMMARY Streptococcal species isolated from dairy cows with clinical mastitis were obtained from mastitis research workers in Florida, Louisiana, New York, Vermont, Washington, and West Virginia. Seventy-one streptococcal isolates were tested, including 39 strains of Streptococcus agalactiae, 21 strains of S dysgalactiae, and 11 strains of S uberis. The minimal inhibitory concentration of erythromycin, lincomycin, oxytetracycline, penicillin, spectinomycin, streptomycin, and tetracycline was determined for each isolate. Differences were not detected among strains with respect to geographic ori

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2

ZADOKS, R. N., R. N. GONZÁLEZ, K. J. BOOR, and Y. H. SCHUKKEN. "Mastitis-Causing Streptococci Are Important Contributors to Bacterial Counts in Raw Bulk Tank Milk." Journal of Food Protection 67, no. 12 (2004): 2644–50. http://dx.doi.org/10.4315/0362-028x-67.12.2644.

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The objective of this study was to probe the contribution of streptococci to the microbial quality of raw milk. Over a 5-month period, bulk tank milk samples from 48 New York State dairy farms were analyzed qualitatively for bacterial ecology and quantitatively for total bacterial, streptococcal, staphylococcal, and gram-negative bacterial counts. Linear regression analysis was used to determine the contribution of differential counts to total bacterial counts. Streptococci, staphylococci, and gram-negative bacteria accounted for 69, 3, and 3% of total bacterial count variability, respectively

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3

SHOME, BIBEK RANJAN, MANI BHUVANA, SUSWETA DAS MITRA, et al. "Multiplex PCR for rapid detection of Streptococcus agalactiae, Streptococcus uberis and Streptococcus dysgalactiae in subclinical mastitis milk." Indian Journal of Animal Sciences 82, no. 10 (2012): 1137–41. http://dx.doi.org/10.56093/ijans.v82i10.24279.

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To improve mastitis diagnosis and achieve rapid, specific, reliable and cost effective test, a multiplex PCR for simultaneous detection and differentiation of major streptococcal species, viz. Streptococcus agalactiae, Streptococcus uberis and Streptococcus dysgalactiae was developed. Evaluation with 24 ATCC strains and 606 strains comprising streptococci (84) and staphylococci (522) showed the assay to be highly accurate. The threshold of detection of the mPCR assay was 10fg of genomic DNA and < 102 CFU ml-1. Assessment of 115 milk samples collected from subclinically infected herd, showed

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4

Shevchenko, M., and A. Andriichuk. "Antibiotic resistance of isolates of Staphylococcus spp. and Streptococcus spp. causing mastitis on dairy farms in Ukraine." Naukovij vìsnik veterinarnoï medicini, no. 1(180) (May 25, 2023): 81–88. http://dx.doi.org/10.33245/2310-4902-2023-180-1-81-88.

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Mastitis is the most common pathology of cows that causes large economic losses to dairy farms. Mastitis is often caused by a group of infectious associated pathogens that can be transmitted among animals. Most often, the pathological process in subclinically and clinically sick animals is caused by coccal gram-positive microflora. A major problem is the mechanisms by which microorganisms acquire resistance to one or more antibacterial agents. Thus, standard treatment regimens used on the farm become ineffective. The publication presents the results of the study of antibiotic resistance of 45

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5

Varhimo, Emilia, Kirsi Savijoki, Jari Jalava, Oscar P. Kuipers, and Pekka Varmanen. "Identification of a Novel Streptococcal Gene Cassette Mediating SOS Mutagenesis in Streptococcus uberis." Journal of Bacteriology 189, no. 14 (2007): 5210–22. http://dx.doi.org/10.1128/jb.00473-07.

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ABSTRACT Streptococci have been considered to lack the classical SOS response, defined by increased mutation after UV exposure and regulation by LexA. Here we report the identification of a potential self-regulated SOS mutagenesis gene cassette in the Streptococcaceae family. Exposure to UV light was found to increase mutations to antibiotic resistance in Streptococcus uberis cultures. The mutational spectra revealed mainly G:C→A:T transitions, and Northern analyses demonstrated increased expression of a Y-family DNA polymerase resembling UmuC under DNA-damaging conditions. In the absence of t

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6

Zouharova, M., K. Nedbalcova, P. Slama, J. Bzdil, M. Masarikova, and J. Matiasovic. "Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitis." Veterinární Medicína 67, No. 3 (2022): 123–30. http://dx.doi.org/10.17221/95/2021-vetmed.

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Streptococcus uberis is one of the most important mastitis-causing pathogens. Although the pathogenesis and virulence factors required for the intramammary infection development are not yet well established, several putative virulence-associated genes have been described. This work aimed to investigate the presence of ten known and putative virulence-associated genes in S. uberis isolated from subclinical or clinical mastitis and its closely related species Streptococcus parauberis in 135 dairy farms in the Czech Republic. The PCR analysis detected that all the examined isolates possessed at l

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7

REIDMILLER, JEFFREY S., WAYNE L. SMITH, MARY M. SAWYER, BENNIE I. OSBURN, JEFFERY L. STOTT, and JAMES S. CULLOR. "Antimicrobial Properties of the Chelating Agent EDTA on Streptococcal Bovine Mastitis Isolates." Journal of Food Protection 69, no. 6 (2006): 1460–62. http://dx.doi.org/10.4315/0362-028x-69.6.1460.

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To determine the efficacy of the chelating agent EDTA on microbial growth, separate cultures of two streptococcal bovine mastitis isolates, Streptococcus agalactiae and Streptococcus uberis, were exposed to known concentrations of EDTA. Bacterial cultures of 108 CFU/ml were exposed to concentrations of EDTA ranging from 30 to 100 mM in an in-vitro-milk environment. Multiple replications of cultures exposed to EDTA were plated during a two-hour time course. A concentration of 100 mM EDTA resulted in a 90% reduction of S. agalactiae and a 99% reduction of S. uberis. Under these experimental cond

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8

Haenni, Marisa, Laure Galofaro, Mathilde Ythier, et al. "Penicillin-Binding Protein Gene Alterations in Streptococcus uberis Isolates Presenting Decreased Susceptibility to Penicillin." Antimicrobial Agents and Chemotherapy 54, no. 3 (2010): 1140–45. http://dx.doi.org/10.1128/aac.00915-09.

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ABSTRACT Streptococcus uberis is an environmental pathogen commonly causing bovine mastitis, an infection that is generally treated with penicillin G. No field case of true penicillin-resistant S. uberis (MIC > 16 mg/liter) has been described yet, but isolates presenting decreased susceptibility (MIC of 0.25 to 0.5 mg/liter) to this drug are regularly reported to our laboratory. In this study, we demonstrated that S. uberis can readily develop penicillin resistance in laboratory-evolved mutants. The molecular mechanism of resistance (acquisition of mutations in penicillin-binding protein 1A

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9

Doane, R. M., S. P. Oliver, R. D. Walker, and E. P. Shull. "Experimental infection of lactating bovine mammary glands with Streptococcus uberis in quarters colonized by Corynebacterium bovis." American Journal of Veterinary Research 48, no. 5 (1987): 749–54. https://doi.org/10.2460/ajvr.1987.48.05.749.

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SUMMARY Twenty-seven quarters of 18 lactating dairy cows were inoculated intramammarily with 3.6 − 104 colony-forming units (cfu) of a strain of Streptococcus uberis isolated from a cow with clinical mastitis. Before quarters were inoculated, 22 were considered as naturally colonized with Corynebacterium bovis, and 5 were considered bacteriologically negative. Streptococcus uberis was isolated from all quarters within 2 days after inoculation, and all quarters developed clinical mastitis by 3 days after inoculation. Mastitis was acute, and most cows had increased rectal temperatures. The numbe

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10

Almeida, Raúl A., Oudessa Kerro Dego, Susan I. Headrick, Mark J. Lewis, and Stephen P. Oliver. "Role of Streptococcus uberis adhesion molecule in the pathogenesis of Streptococcus uberis mastitis." Veterinary Microbiology 179, no. 3-4 (2015): 332–35. http://dx.doi.org/10.1016/j.vetmic.2015.07.005.

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11

Jiang, Min, P. Ronald MacLachlan, Lorne A. Babiuk, Alexandra J. Bolton, and Andrew A. Potter. "The abp locus of Streptococcus uberis encodes a protein homologous to polar amino acid and opine binding proteins of Gram-negative bacteria." Canadian Journal of Microbiology 44, no. 8 (1998): 784–88. http://dx.doi.org/10.1139/w98-058.

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A gene locus abp was identified immediately upstream of the CAMP factor gene cfu in Streptococcus uberis. An open reading frame capable of coding for a 277-residue protein was identified. On the basis of sequence characteristics, the abp gene product is potentially a polar amino acid and opine binding component of an ATP-binding cassette type (ABC-type) transport system similar to those of Gram-negative bacteria. This membrane protein is likely lipid modified at its amino terminus and was present in five S. uberis strains and one Streptococcus parauberis strain examined.Key words: bovine masti

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12

Khan, Sohrab, Tian Wang, Eduardo R. Cobo, et al. "Antioxidative Sirt1 and the Keap1-Nrf2 Signaling Pathway Impair Inflammation and Positively Regulate Autophagy in Murine Mammary Epithelial Cells or Mammary Glands Infected with Streptococcus uberis." Antioxidants 13, no. 2 (2024): 171. http://dx.doi.org/10.3390/antiox13020171.

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Streptococcus uberis mastitis in cattle infects mammary epithelial cells. Although oxidative responses often remove intracellular microbes, S. uberis survives, but the mechanisms are not well understood. Herein, we aimed to elucidate antioxidative mechanisms during pathogenesis of S. uberis after isolation from clinical bovine mastitis milk samples. S. uberis’s in vitro pathomorphology, oxidative stress biological activities, transcription of antioxidative factors, inflammatory response cytokines, autophagosome and autophagy functions were evaluated, and in vivo S. uberis was injected into the

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13

Lan, Riguo, Yuanyuan Zhou, Zhenglei Wang та ін. "Reduction of ROS-HIF1α-driven glycolysis by taurine alleviates Streptococcus uberis infection". Food & Function 13, № 4 (2022): 1774–84. http://dx.doi.org/10.1039/d1fo03909a.

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14

Maćešić, Nino, Tihana Fumić, Sanja Duvnjak, et al. "Agreement of conventional microbiological and molecular identification of streptococci isolated from bovine milk." Veterinarski arhiv 92, no. 4 (2022): 381–87. http://dx.doi.org/10.24099/vet.arhiv.1574.

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Pathogenic streptococci are implicated in clinical and subclinical mastitis. Most laboratories identify streptococci on the basis of microbiological examination, but molecular diagnostic methods have become the gold standard of mastitis diagnosis in the last few years. Therefore, this study aims to determine the agreement of microbiological and molecular identification of streptococci isolates from bovine milk. Milk samples were taken before the evening milking into sterile tubes. Samples were examined bacteriologically by inoculation on aesculin blood agar. Identification of grown colonies wa

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15

Dmitriev, A., M. Bhide, and I. Mikula. "cpn60 Gene-Based Multiplex-PCR Assay for Simultaneous Identification of Streptococcal Species." Acta Veterinaria Brno 75, no. 2 (2006): 235–40. http://dx.doi.org/10.2754/avb200675020235.

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The cpn60 genes of Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis were sequenced and a certain polymorphism of cpn60 genes was revealed. Presumable species-specific pairs of primers were designed and their specificity was confirmed by PCR. Based on these data, the cpn60 gene-based multiplex-PCR assay was developed. It was found to be effective for simultaneous identification of S. agalactiae, S. dysgalactiae and S. uberis strains.

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16

Laven, Richard. "Disease Facts -Streptococcus uberis mastitis." Livestock 16, no. 3 (2011): 39–40. http://dx.doi.org/10.1111/j.2044-3870.2011.00037.x.

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17

Zhang, Tingrui, Duangporn Pichpol, and Sukolrat Boonyayatra. "Application of MALDI-TOF MS to Identify and Detect Antimicrobial-Resistant Streptococcus uberis Associated with Bovine Mastitis." Microorganisms 12, no. 7 (2024): 1332. http://dx.doi.org/10.3390/microorganisms12071332.

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Streptococcus uberis is a common bovine mastitis pathogen in dairy cattle. The rapid identification and characterization of antimicrobial resistance (AMR) in S. uberis plays an important role in its diagnosis, treatment, and prevention. In this study, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify S. uberis and screen for potential AMR biomarkers. Streptococcus uberis strains (n = 220) associated with bovine mastitis in northern Thailand were identified using the conventional microbiological methods and compared with the results

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18

Li, Bin, Zhixin Wan, Zhenglei Wang, et al. "TLR2 Signaling Pathway Combats Streptococcus uberis Infection by Inducing Mitochondrial Reactive Oxygen Species Production." Cells 9, no. 2 (2020): 494. http://dx.doi.org/10.3390/cells9020494.

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Mastitis caused by Streptococcus uberis (S. uberis) is a common and difficult-to-cure clinical disease in dairy cows. In this study, the role of Toll-like receptors (TLRs) and TLR-mediated signaling pathways in mastitis caused by S. uberis was investigated using mouse models and mammary epithelial cells (MECs). We used S. uberis to infect mammary glands of wild type, TLR2−/− and TLR4−/− mice and quantified the adaptor molecules in TLR signaling pathways, proinflammatory cytokines, tissue damage, and bacterial count. When compared with TLR4 deficiency, TLR2 deficiency induced more severe pathol

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19

Vakanjac, Slobodanka, Vojislav Pavlovic, Vladimir Magas, et al. "Investigations of efficacy of intramammary applied antimicrobials and glucocorticosteroides in the treatment of subclinical and clinical mastitis in cows." Veterinarski glasnik 67, no. 1-2 (2013): 15–27. http://dx.doi.org/10.2298/vetgl1302015v.

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Inflammation of the mammary gland, mastitis in cows, presents one of the most acute problems in intensive dairy production, inflicting huge economic losses. In the course of one year, 80 samples were taken at investigated farms from udder quarters of cows with clinical mastitis and 160 samples from udder quarters of cows with subclinical mastitis. The efficacy of three preparations, A, B, and C, was examined in the treatment of clinical and subclinical mastitis in cows. The investigations indicate that antibiotic preparation A (neomycin, polimixine B, oleandomycin and prednisolone) exhibited a

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20

Phuektes, Patchara, Glenn F. Browning, Garry Anderson, and Peter D. Mansell. "Multiplex polymerase chain reaction as a mastitis screening test for Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis in bulk milk samples." Journal of Dairy Research 70, no. 2 (2003): 149–55. http://dx.doi.org/10.1017/s0022029903006010.

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Effective diagnostic tools for screening herds for mastitis pathogens are important in development and monitoring of mastitis control programmes. A multiplex polymerase chain reaction (PCR) assay for simultaneous detection of Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis was used in preliminary studies to assess its applicability as an alternative method for monitoring mastitis caused by these organisms at the herd level. PCR was used to detect the presence of these organisms in bulk milk samples. Correlations with bulk milk somatic cell c

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21

Sladek, Z., D. Rysanek, H. Ryznarova, and M. Faldyna. "The role of neutrophil apoptosis during experimentally induced Streptococcus uberismastitis ." Veterinární Medicína 51, No. 9 (2012): 437–47. http://dx.doi.org/10.17221/5571-vetmed.

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The object of the study was to determine if apoptosis of neutrophils and their subsequent elimination from the mammary gland by macrophages are modulated by an infection of Streptococcus uberis. The experiments were carried out in 5 clinically normal Holstein &times; Bohemian Red Pied crossbred heifers, aged 14 to 18 months. Before the experimental infection mammary glands were stimulated by PBS as a control. The samples of cell populations were obtained by lavages of the mammary glands in 4 intervals (24, 48, 72 and 168 h) after the PBS and after the experimental infection. Flow cytometry

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22

Bolte, Josef, Yanchao Zhang, Nicole Wente, and Volker Krömker. "In Vitro Susceptibility of Mastitis Pathogens Isolated from Clinical Mastitis Cases on Northern German Dairy Farms." Veterinary Sciences 7, no. 1 (2020): 10. http://dx.doi.org/10.3390/vetsci7010010.

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The present research study investigated the susceptibility of common mastitis pathogens—obtained from clinical mastitis cases on 58 Northern German dairy farms—to routinely used antimicrobials. The broth microdilution method was used for detecting the Minimal Inhibitory Concentration (MIC) of Streptococcus agalactiae (n = 51), Streptococcus dysgalactiae (n = 54), Streptococcus uberis (n = 50), Staphylococcus aureus (n = 85), non-aureus staphylococci (n = 88), Escherichia coli (n = 54) and Klebsiella species (n = 52). Streptococci and staphylococci were tested against cefquinome, cefoperazone,

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23

Heng, Nicholas C. K., Grace A. Burtenshaw, Ralph W. Jack, and John R. Tagg. "Ubericin A, a Class IIa Bacteriocin Produced by Streptococcus uberis." Applied and Environmental Microbiology 73, no. 23 (2007): 7763–66. http://dx.doi.org/10.1128/aem.01818-07.

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ABSTRACT Streptococcus uberis, a causal agent of bovine mastitis, produces ubericin A, a 5.3-kDa class IIa (pediocin-like) bacteriocin, which was purified and characterized. The uba locus comprises two overlapping genes: ubaA (ubericin A precursor peptide) and ubaI (putative immunity protein). Ubericin A is the first streptococcal class IIa bacteriocin to be characterized.

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24

Murdoch, David R., Lois M. Seawand, and Martin R. MacFarlane. "Human wound infection with streptococcus uberis." Clinical Microbiology Newsletter 19, no. 22 (1997): 174–75. http://dx.doi.org/10.1016/s0196-4399(00)89187-4.

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25

Iqomah, Meta, Alek Arisona, Imawan Daru Prasetya, Adretta Soedarmanto, Yanuartono, and Soedarmanto Indarjulianto. "Mini Review: Lactoferrin-binding protein of Streptococcus in Bovine Mastitis." BIO Web of Conferences 49 (2022): 01008. http://dx.doi.org/10.1051/bioconf/20224901008.

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Bovine mastitis is an udder inflammation mostly found in dairy cattle that causes enormous economic losses. Streptococcus is a bacterium that is often found in mastitis, including Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis. These three species have lactoferrinbinding protein (LBP) as one of their virulence factors. Lactoferrin is a host innate immune protein that acts as antibacterial, immunomodulator, anti-adhesion, and has iron-binding properties. The LBP on the surface of Streptococcus could bind to lactoferrin produced by host cells. Uniquely, the three

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26

OZ, HALIT H., and R. J. FARNSWORTH. "Influence of Addition of Newly Drawn Milk and Fluctuating Temperatures of Farm Bulk Tanks on Growth of Mastitis-Causing Bacteria." Journal of Food Protection 49, no. 1 (1986): 54–57. http://dx.doi.org/10.4315/0362-028x-49.1.54.

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Effect of addition of newly drawn fresh milk of consecutive milkings on growth of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis in milk held at fluctuating temperatures of a farm bulk tank for 48 h was studied. There was a statistically insignificant effect of the addition of newly drawn fresh milk of consecutive milkings on the growth rate of S. aureus, S. epidermidis, S. agalactiae and S. uberis but there was a significant (p&lt;0.001) growth enhancing effect on S. dysgalactiae. However, all the bacteria

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27

Shewmaker, P. Lynn, Alvin C. Camus, Tim Bailiff, Arnold G. Steigerwalt, Roger E. Morey, and Maria da Glória S. Carvalho. "Streptococcus ictaluri sp. nov., isolated from Channel Catfish Ictalurus punctatus broodstock." International Journal of Systematic and Evolutionary Microbiology 57, no. 7 (2007): 1603–6. http://dx.doi.org/10.1099/ijs.0.64810-0.

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A streptococcal-like organism was associated with diseased Channel Catfish Ictalurus punctatus broodstock on four commercial aquaculture operations in the Mississippi Delta. Conventional biochemical testing, 16S rRNA gene sequence analysis and DNA–DNA hybridization distinguished the isolates from these fish from previously published Streptococcus species. Comparative 16S rRNA gene sequencing studies revealed that the isolates were phylogenetically most similar to Streptococcus iniae, Streptococcus uberis and Streptococcus parauberis with divergence ranging from 2.0 to 2.3 %. Streptococcus pyog

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Zouharova, Monika, Katerina Nedbalcova, Katarina Matiaskova, Petr Slama, and Jan Matiasovic. "Antimicrobial Susceptibility and Resistance Genes in Streptococcus uberis Isolated from Bovine Mastitis in the Czech Republic." Antibiotics 12, no. 10 (2023): 1527. http://dx.doi.org/10.3390/antibiotics12101527.

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Streptococcus uberis is one of the most important causative agents of mastitis and is a common reason for the use of antimicrobials in dairy cows. In this study, we assessed the antimicrobial susceptibility of 667 S. uberis isolates originating from 216 Czech dairy farms collected between 2019 and 2023 using the broth microdilution method. We tested 140 of the isolates for the presence of antimicrobial genes using whole-genome sequencing and evaluated their relationship with phenotypic resistance. Streptococcus uberis isolates showed high levels of resistance to tetracycline (59%), followed by

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Hoque, Md Nazmul, Monira Rahman, Taniya Sultana, et al. "Molecular Characterization of Streptococcus spp. Isolated from Milk, Feces and Farm Environment of Mastitic Dairy Cows." Journal of Science and Technology Research 6, no. 1 (2024): 1–12. https://doi.org/10.3329/jscitr.v6i1.77358.

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Streptococci are the primary cause of mastitis, significantly impacting the dairy industry economically. This study aimed to explore the molecular epidemiology, antimicrobial resistance, and virulence characteristics of Streptococcus spp. isolated from the milk, feces, and environment of dairy cows with mastitis. Sixty (60) samples (milk = 20, feces = 20 and soil = 20) from cows with clinical mastitis (CM) were purposively collected and examined. Samples were enriched in Luria Bertani broth (LB) and Streptococcus spp. was isolated on Modified Edwards Medium and confirmed by ribosomal (16S rRNA

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30

Zhang, Yanchao, Nicole Wente, Stefanie Leimbach, et al. "In vitro capsule or biofilm formation of Streptococcus uberis and bacteriological cure of bovine mastitis." Tierärztliche Praxis Ausgabe G: Großtiere / Nutztiere 52, no. 05 (2024): 264–70. http://dx.doi.org/10.1055/a-2410-1465.

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Abstract Objective The relationship between the in vitro detected virulence factors biofilm and capsule formation of Streptococcus (S.) uberis isolates of clinical mastitis in dairy cows and the bacteriological cure rate after antibiotic therapy was investigated in order to better understand the importance of these virulence factors for the bacteriological cure rate. Material and methods A total of 111 clinical mastitis (CM) cases were collected, in which S. uberis was bacteriologically detected. All mastitis cases were treated in accordance with the approval conditions of the antibiotic udder

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Stempler, A., A. J. Muñoz, and M. F. Lucas. "Streptococcus uberis y su importancia como agente causal de la mastitis bovina." Revista Veterinaria 33, no. 2 (2022): 192. http://dx.doi.org/10.30972/vet.3326181.

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A nivel nacional e internacional, la prevalencia relativa de mastitis causada por Streptococcus uberis fue aumentando a lo largo del tiempo. S. uberis ha sido descripto como agente causal de mastitis bovina de origen ambiental, aunque se lo considera potencialmente contagioso. Los términos “cepas bien adaptadas” o “cepas poco adaptadas” al hospedador son utilizados para referirse a cepas contagiosas o ambientales, respectivamente. La incorporación de técnicas moleculares ha permitido caracterizar distintos perfiles genéticos de S. uberis como factores de virulencia asociados a su patogenicidad

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WANG, S. M., M. A. DEIGHTON, J. A. CAPSTICK, and N. GERRATY. "Epidemiological typing of bovine streptococci by pulsed-field gel electrophoresis." Epidemiology and Infection 123, no. 2 (1999): 317–24. http://dx.doi.org/10.1017/s0950268899002745.

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Pulsed-field gel electrophoresis (PFGE) was used to investigate the epidemiology of streptococcal mastitis in dairy cattle. The most prevalent streptococcal species, Streptococcus uberis (60–80% of streptococcal isolates), was highly heterogeneous, with different cows only rarely sharing the same pulsotype. S. agalactiae was rarely encountered, however all eight isolates from one farm generated identical PFGE profiles, which differed from those of all other isolates examined, confirming cow-to-cow transmission. Fifty-two isolates of S. dysgalactiae from 27 cows on 5 farms generated 6 different

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33

Kabelitz, Tina, Etienne Aubry, Kira van Vorst, Thomas Amon, and Marcus Fulde. "The Role of Streptococcus spp. in Bovine Mastitis." Microorganisms 9, no. 7 (2021): 1497. http://dx.doi.org/10.3390/microorganisms9071497.

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The Streptococcus genus belongs to one of the major pathogen groups inducing bovine mastitis. In the dairy industry, mastitis is the most common and costly disease. It not only negatively impacts economic profit due to milk losses and therapy costs, but it is an important animal health and welfare issue as well. This review describes a classification, reservoirs, and frequencies of the most relevant Streptococcus species inducing bovine mastitis (S. agalactiae, S. dysgalactiae and S. uberis). Host and environmental factors influencing mastitis susceptibility and infection rates will be discuss

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34

Sherwin, Virginia E., Sharon A. Egan, Martin J. Green, and James A. Leigh. "Survival of Streptococcus uberis on bedding substrates." Veterinary Journal 276 (October 2021): 105731. http://dx.doi.org/10.1016/j.tvjl.2021.105731.

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35

Leigh, James A. "Activation of bovine plasminogen by Streptococcus uberis." FEMS Microbiology Letters 114, no. 1 (1993): 67–71. http://dx.doi.org/10.1111/j.1574-6968.1993.tb06552.x.

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36

Lämmler, C. "Biochemical and Serological Properties of Streptococcus uberis." Journal of Veterinary Medicine, Series B 38, no. 1-10 (1991): 737–42. http://dx.doi.org/10.1111/j.1439-0450.1991.tb00937.x.

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37

Wirawan, Ruth E., Nikolai A. Klesse, Ralph W. Jack, and John R. Tagg. "Molecular and Genetic Characterization of a Novel Nisin Variant Produced by Streptococcus uberis." Applied and Environmental Microbiology 72, no. 2 (2006): 1148–56. http://dx.doi.org/10.1128/aem.72.2.1148-1156.2006.

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ABSTRACT Streptococcus uberis is one of the principal causative agents of bovine mastitis. In this study, we report that S. uberis strain 42 produces a lantibiotic, nisin U, which is 78% identical (82% similar) to nisin A from Lactococcus lactis. The 15.6-kb nisin U locus comprises 11 open reading frames, similar in putative functionality but differing in arrangement from that of the nisin A biosynthetic cluster. The nisin U producer strain exhibits specific resistance (immunity) to nisin U and cross-resistance to nisin A, a finding consistent with the 55% sequence similarity of their respecti

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Almeida, Raúl A., Oudessa Kerro-Dego, and Agustín G. Rius. "Effect of heat stress on the interaction of Streptococcus uberis with bovine mammary epithelial cells." Journal of Dairy Research 85, no. 1 (2018): 53–56. http://dx.doi.org/10.1017/s0022029917000875.

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Heat stress (HS) negatively affects milk production and has been associated with decreased immune function, and increased rate of intramammary infections (IMI). Research has shown that HS affects gene expression, cell cycle, and cell metabolism in bovine mammary epithelial cells (BMEC). Since BMEC are an initial target of mastitis pathogens, we studied adherence to and internalisation of S. uberis into HS-BMEC, as well as the effect that this interaction has on host cells by measuring HS-BMEC viability and membrane integrity. Results reported in this Research Communication showed that HS reduc

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Denham, E. L., P. N. Ward, and J. A. Leigh. "Lipoprotein Signal Peptides Are Processed by Lsp and Eep of Streptococcus uberis." Journal of Bacteriology 190, no. 13 (2008): 4641–47. http://dx.doi.org/10.1128/jb.00287-08.

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ABSTRACT Lipoprotein signal peptidase (lsp) is responsible for cleaving the signal peptide sequence of lipoproteins in gram-positive bacteria. Investigation of the role of Lsp in Streptococcus uberis, a common cause of bovine mastitis, was undertaken using the lipoprotein MtuA (a protein essential for virulence) as a marker. The S. uberis lsp mutant phenotype displayed novel lipoprotein processing. Not only was full-length (uncleaved) MtuA detected by Western blotting, but during late log phase, a lower-molecular-weight derivative of MtuA was evident. Similar analysis of an S. uberis double mu

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Janevsкi, Aleksandar, Iskra Cvetkovikj, Sanja Kiprijanovska, et al. "Prevalence of Subclinical Mastitis Pathogens in Small Dairy Farms in Republic of North Macedonia." Macedonian Veterinary Review 43, no. 1 (2020): 23–30. http://dx.doi.org/10.2478/macvetrev-2020-0010.

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AbstractSubclinical mastitis is an asymptomatic udder infection distributed worldwide with enormous losses in the dairy industry. The study’s objective was to determine the presence of this pathological condition in small dairy farms in the R. of N. Macedonia and to identify the most common associated bacteria. Milk samples were obtained from 96 dairy cows (378 udder quarters) in seven dairy farms, in 3 consecutive samplings 24–72 hours apart. The samples were cultured on routine bacteriological growth media and incubated for 24–48 hours. The isolates were identified by AximaiD Plus MALDITOF M

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Abd El-Aziz, Norhan K., Ahmed M. Ammar, Hend M. El Damaty, et al. "Environmental Streptococcus uberis Associated with Clinical Mastitis in Dairy Cows: Virulence Traits, Antimicrobial and Biocide Resistance, and Epidemiological Typing." Animals 11, no. 7 (2021): 1849. http://dx.doi.org/10.3390/ani11071849.

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Mastitis remains a serious problem for dairy animals. The misappropriation of antimicrobial agents helps accelerate resistance, which poses a serious challenge in controlling environmental S. uberis infection. Here, we study the virulence attributes, antimicrobial and biocide resistance, and epidemiological typing of S. uberis recovered from bovine clinical mastitis in dairy farms of diverse hygienic interventions in Egypt. The overall S. uberis infection rate was 20.59%; all were multidrug-resistant (MDR). The sua gene was the most frequent virulence gene (42.02%), followed by pauA (40.57%),

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Sherwin, Virginia E., Morena Santi, Olivia Walker, et al. "PCR-Based Direct Detection of Streptococcus uberis from Subclinical and Clinical Dairy Cattle Milk Samples." Veterinary Medicine International 2020 (December 8, 2020): 1–9. http://dx.doi.org/10.1155/2020/8828624.

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Streptococcus uberis is one of the leading causes worldwide of mastitis in the dairy industry, with the most likely sources of infection attributed to environmental reservoirs such as contaminated bedding materials. Early detection of those cases most likely to progress to clinical disease would lead to improved animal welfare, a critical component of overall health and productivity. A multiplex PCR-based diagnostic test was developed for detection of S. uberis directly from milk and targeting two genes previously identified as important for intramammary colonisation and persistence in dairy c

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Fidelis, Carlos E., Alessandra M. Orsi, Gustavo Freu, Juliano L. Gonçalves, and Marcos V. dos Santos. "Biofilm Formation and Antimicrobial Resistance of Staphylococcus aureus and Streptococcus uberis Isolates from Bovine Mastitis." Veterinary Sciences 11, no. 4 (2024): 170. http://dx.doi.org/10.3390/vetsci11040170.

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This study aimed to assess (a) the biofilm producer ability and antimicrobial resistance profiles of Staphylococcus (Staph.) aureus and Streptococcus (Strep.) uberis isolated from cows with clinical mastitis (CM) and subclinical mastitis (SCM), and (b) the association between biofilm producer ability and antimicrobial resistance. We isolated a total of 197 Staph. aureus strains (SCM = 111, CM = 86) and 119 Strep. uberis strains (SCM = 15, CM = 104) from milk samples obtained from 316 cows distributed in 24 dairy herds. Biofilm-forming ability was assessed using the microplate method, while ant

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ERYILDIZ, Canan, Şebnem BUKAVAZ, Şaban GÜRCAN, and Osman HATİPOĞLU. "Mikrobiyoloji Laboratuvarlarında Nadir İzole Edilen Bir Etken: Streptococcus uberis." Mikrobiyoloji Bulteni 51, no. 2 (2017): 171–76. http://dx.doi.org/10.5578/mb.40378.

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Thomas, Funmilola Clara, William Mullen, Riccardo Tassi, et al. "Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 1. High abundance proteins, acute phase proteins and peptidomics." Molecular BioSystems 12, no. 9 (2016): 2735–47. http://dx.doi.org/10.1039/c6mb00239k.

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Kerro Dego, Oudessa, Raul Almeida, Susan Ivey, and Getahun E. Agga. "Evaluation of Streptococcus uberis Surface Proteins as Vaccine Antigens to Control S. uberis Mastitis in Dairy Cows." Vaccines 9, no. 8 (2021): 868. http://dx.doi.org/10.3390/vaccines9080868.

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There is no effective vaccine against Streptococcus uberis mastitis in dairy cows. Objectives of this study were (1) to extract S. uberis surface proteins (SUSP) and determine immunoreactivity in vitro and (2) immunogenicity and efficacy in vivo. SUSP was extracted from S. uberis, and their immunoreactivity was tested by western blot. In total, 26 Jersey dairy cows were randomly divided into four groups. Groups 1, 2, and 3 were vaccinated subcutaneously with 4 mg, 1 mg, and 100 μg of SUSP, respectively, with Freund’s incomplete adjuvant. Group 4 (control) was injected with placebo. S. uberis U

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ZADOKS, R. N., B. E. GILLESPIE, H. W. BARKEMA, O. C. SAMPIMON, S. P. OLIVER, and Y. H. SCHUKKEN. "Clinical, epidemiological and molecular characteristics of Streptococcus uberis infections in dairy herds." Epidemiology and Infection 130, no. 2 (2003): 335–49. http://dx.doi.org/10.1017/s0950268802008221.

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A longitudinal observational study (18 months) was carried out in two Dutch dairy herds to explore clinical, epidemiological and molecular characteristics of Streptococcus uberis mastitis. Infections (n=84) were detected in 70 quarters of 46 cows. Bacterial isolates were characterized at strain level by random amplified polymorphic DNA (RAPD) fingerprinting. Persistent infections were usually attributable to one strain, while recurrent infections could be caused by different strains. When multiple quarters of a cow were infected, infections were mostly caused by one strain. In each herd, multi

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Collins, Robert A., Keith R. Parsons, Terry R. Field, and A. John Bramley. "Histochemical localization and possible antibacterial role of xanthine oxidase in the bovine mammary gland." Journal of Dairy Research 55, no. 1 (1988): 25–32. http://dx.doi.org/10.1017/s0022029900025814.

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SummaryXanthine oxidase (XO) was demonstrated to be present in the teat canal and secretory tissue of the bovine mammary gland by histochemical techniques. Homogenates of these tissues were able to replace XO in an antibacterial assay with Streptococcus uberis. The action of XO on its substrate hypoxanthine was shown to provide an essential component for anti-streptococcal activity mediated by lactoperoxidase. A mechanism is proposed whereby the interaction of XO, lactoperoxidase and thiocyanate may provide antibacterial activity in the teat canal.

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Zadoks, R. N., H. G. Allore, H. W. Barkema, O. C. Sampimon, Y. T. Gröhn, and Y. H. Schukken. "Analysis of an Outbreak of Streptococcus uberis Mastitis." Journal of Dairy Science 84, no. 3 (2001): 590–99. http://dx.doi.org/10.3168/jds.s0022-0302(01)74512-2.

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Wolter, Wilfried. "Besonderheiten und strategische Bekämpfung von Streptococcus-uberis-Mastitiden." veterinär spiegel 22, no. 02 (2012): 75–80. http://dx.doi.org/10.1055/s-0031-1298526.

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