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1

Keinath, Anthony P., and Rosemary Loria. "Melanin-producing Streptomyces spp. respond to potato plant growth and differentially to potato cultivars." Canadian Journal of Microbiology 36, no. 4 (1990): 279–85. http://dx.doi.org/10.1139/m90-048.

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Population dynamics of Streptomyces spp. producing melanoid pigments were monitored in field plots planted to the potato cultivars 'Chippewa' (susceptible to common scab) or 'Superior' (resistant), and in fallow control plots. Relative frequencies of streptomycetes most commonly isolated from soil, the rhizosphere, and potato tuber surfaces were determined during two growing seasons. Shannon indices of diversity indicated populations in soils planted to potatoes were more diverse than populations in fallow soil. In 1986, S. diastatochromogenes and S. longisporus accounted for ≥38% of all strep
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2

Neeno-Eckwall, Eric C., Linda L. Kinkel, and Janet L. Schottel. "Competition and antibiosis in the biological control of potato scab." Canadian Journal of Microbiology 47, no. 4 (2001): 332–40. http://dx.doi.org/10.1139/w01-010.

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Nonpathogenic, antibiotic-producing streptomycetes have been shown to reduce potato scab when added to disease-conducive soil. Spontaneous mutants of the pathogenic Streptomyces scabies RB4 that are resistant to at least one antibiotic activity produced by the nonpathogenic suppressive isolates Streptomyces diastatochromogenes strain PonSSII and S. scabies PonR have been isolated. To determine the importance of antibiosis in this biocontrol system, these mutants were investigated for their ability to cause disease in the presence of the two pathogen antagonists in a greenhouse assay. Disease c
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3

Meng, Qingxiao, Jingfang Yin, Noah Rosenzweig, David Douches, and Jianjun J. Hao. "Culture-Based Assessment of Microbial Communities in Soil Suppressive to Potato Common Scab." Plant Disease 96, no. 5 (2012): 712–17. http://dx.doi.org/10.1094/pdis-05-11-0441.

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A field in East Lansing, MI, showed a decline of potato common scab compared with an adjacent potato field. To confirm that the decline was due to biological factors, the soil was assayed. In the greenhouse, putative common-scab-suppressive soil (SS) was either treated with various temperatures or mixed with autoclaved SS at various ratios. Pathogenic Streptomyces scabies was incorporated into the treated soil at 106 CFU/cm3 of soil, followed by planting of either potato or radish. Disease severity was negatively correlated with the percentage of SS in the mixture and positively correlated wit
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4

Bowers, John H., Linda L. Kinkel, and Roger K. Jones. "Influence of disease-suppressive strains of Streptomyces on the native Streptomyces community in soil as determined by the analysis of cellular fatty acids." Canadian Journal of Microbiology 42, no. 1 (1996): 27–37. http://dx.doi.org/10.1139/m96-005.

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Analysis of cellular fatty acid profiles was used to distinguish among introduced pathogen- suppressive strains and indigenous strains of Streptomyces spp. isolated from soil of field plots established to test the efficacy of Streptomyces strains PonSSII and PonR in the biological control of potato scab. Reference libraries of fatty acid profiles were developed for a collection of known pathogenic strains and the introduced suppressive strains. Population densities of pathogen-related, suppressive, and saprophytic Streptomyces strains were determined from the relationship of field isolates to
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5

Nikulina, A. N., N. A. Ryabova, Y. Lu, and A. A. Zimin. "A New Bacteriophage Of The Family Siphoviridae Isolated From The Soddy-Podzolic Soils Of The Prioksko-Terrasny Nature Reserve." GEOGRAPHY, ENVIRONMENT, SUSTAINABILITY 16, no. 1 (2023): 111–18. http://dx.doi.org/10.24057/2071-9388-2022-050.

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Bacteria of the genus Streptomyces, one of the main microorganisms of soils, and their bacteriophages are important inhabitants of soil ecosystems. Important though they are, not much is known about their functional patterns and population dynamics. A question of particular interest, which is still to be understood, is how bacteriophages regulate the population dynamics of Streptomyces and how this regulation affects the soil ecosystem as a whole. Isolation and study of new Streptomyces bacteriophages can help to understand these problems. In this paper, we describe isolation of a new bacterio
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6

Cheng, Kun, Xiaoying Rong, Adrián A. Pinto-Tomás, Marcela Fernández-Villalobos, Catalina Murillo-Cruz, and Ying Huang. "Population Genetic Analysis of Streptomyces albidoflavus Reveals Habitat Barriers to Homologous Recombination in the Diversification of Streptomycetes." Applied and Environmental Microbiology 81, no. 3 (2014): 966–75. http://dx.doi.org/10.1128/aem.02925-14.

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ABSTRACTExamining the population structure and the influence of recombination and ecology on microbial populations makes great sense for understanding microbial evolution and speciation. Streptomycetes are a diverse group of bacteria that are widely distributed in nature and a rich source of useful bioactive compounds; however, they are rarely subjected to population genetic investigations. In this study, we applied a five-gene-based multilocus sequence analysis (MLSA) scheme to 41 strains ofStreptomyces albidoflavusderived from diverse sources, mainly insects, sea, and soil. Frequent recombin
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7

BOSTUBAEVA, Makpal B., Ainash P. NAUANOVA, and Rıdvan KIZILKAYA. "Influence of inoculating microbes on municipal sewage sludge composting." EURASIAN JOURNAL OF SOIL SCIENCE (EJSS) 11, no. 4 (2022): 295–302. http://dx.doi.org/10.18393/ejss.1114772.

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The influence of Ilkompost and Micromix bacterial consortium inoculation during sewage sludge (SS) with wheat straw (WS) composting was assessed. The effect of inoculation on compost quality parameters such as pH, temperature, nutrient contents and C/N, bacterial and fungal population were determined. Compared to the control treatment, the temperature of piles and population of microorganism increased after inoculated bacterial consortiums at the beginning of compost. But, WS addition did not effect on compost quality parameters and microbial population. Fungal and bacterial population, the pea
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8

Conn, Kenneth L., Edlira Leci, Giora Kritzman, and George Lazarovits. "A Quantitative Method for Determining Soil Populations of Streptomyces and Differentiating Potential Potato Scab-Inducing Strains." Plant Disease 82, no. 6 (1998): 631–38. http://dx.doi.org/10.1094/pdis.1998.82.6.631.

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A procedure is described for estimating Streptomyces populations in soil. Soils are air-dried, 10g quantities are shaken in plastic bags containing 0.1% water agar and homogenized with a Stomacher homogenizer, serial dilutions are plated on a semi-selective culture (STR) medium and incubated for 2 weeks at 22°C, and the Streptomyces colonies are enumerated. Use of STR medium reduced the bacterial and fungal colonies recovered from soil to levels below that of the Streptomyces spp. while not affecting the number of Streptomyces colonies compared with those enumerated on yeast malt extract mediu
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9

Dolya, B., T. Busche, T. S. Jørgensen, et al. "A database of sequenced genomes of different Streptomyces albus J1074 strains and uses thereof." Visnyk of Lviv University. Biological series, no. 85 (February 25, 2021): 26–34. http://dx.doi.org/10.30970/vlubs.2021.85.03.

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Streptomyces albus J1074 has recently emerged as a powerful chassis strain for natural product research and discovery, as well as model to investigate various aspects of actinobacterial biology. A number of genetic tools have been developed to facilitate the use of this strain for the aforementioned purposes. One of the promising approaches is to introduce into J1074 genome mutations that would improve the antibiotic-producing capacity of J1074. Particularly, we reported a collection of spontaneous and genomically engineered J1074 mutants carrying mutation in genes for ribosomal protein S12, R
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10

Tomihama, Tsuyoshi, Yatsuka Nishi, Kiyofumi Mori, et al. "Rice Bran Amendment Suppresses Potato Common Scab by Increasing Antagonistic Bacterial Community Levels in the Rhizosphere." Phytopathology® 106, no. 7 (2016): 719–28. http://dx.doi.org/10.1094/phyto-12-15-0322-r.

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Potato common scab (PCS), caused by pathogenic Streptomyces spp., is a serious disease in potato production worldwide. Cultural practices, such as optimizing the soil pH and irrigation, are recommended but it is often difficult to establish stable disease reductions using these methods. Traditionally, local farmers in southwest Japan have amended soils with rice bran (RB) to suppress PCS. However, the scientific mechanism underlying disease suppression by RB has not been elucidated. The present study showed that RB amendment reduced PCS by repressing the pathogenic Streptomyces population in y
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11

Frändberg, Emma, and Johan Schnürer. "Antifungal activity of chitinolytic bacteria isolated from airtight stored cereal grain." Canadian Journal of Microbiology 44, no. 2 (1998): 121–27. http://dx.doi.org/10.1139/w97-141.

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Chitinolytic bacteria are used as biocontrol agents of plant pathogenic fungi. They might also potentially inhibit growth of molds, e.g., Aspergillus spp. and Penicillium spp., in stored plant material. We isolated chitinolytic bacteria from airtight stored cereal grain and evaluated their antifungal capacity. Between 0.01 and 0.5% of the total aerobic counts were chitinolytic bacteria. Gram-negative bacteria, mainly Pseudomonadaceae, constituted approximately 80% of the chitinolytic population. Gram-positive isolates belonged predominantly to the Corynebacterium-Arthrobacter group, Streptomyc
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12

Natsagdorj, Oyunbileg, Indra Nyamjav, Byambasuren Davaasambuu, Ariunzaya Jargalsaikhan, and Batsukh Chultem. "The result of effective microorganisms isolated from wastewater and soil." Mongolian Journal of Agricultural Sciences 32, no. 1 (2021): 38–44. http://dx.doi.org/10.5564/mjas.v32i1.1601.

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Increasing urbanization and population concentrations are causing environmental problems such as soil and water pollution. In this study, we were present isolation and identification of Streptomyces sp and Rhodobacteria sp. Those effective microorganisms isolated from the wastewater and soil sample of Anand-Khujirt spring resort located in Khujirt soum, Uvurkhangai province in Mongolia. Based on morphological, cultural, and molecular characteristics (16S rDNA sequencing), those strain were identified as Rhodobacter sphaeroides (99% similarity) and Streptomyces cf.griseus (99% similarity). Rhod
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13

Zhang, Yucheng, and Rosemary Loria. "Emergence of Novel Pathogenic Streptomyces Species by Site-Specific Accretion and cis-Mobilization of Pathogenicity Islands." Molecular Plant-Microbe Interactions® 30, no. 1 (2017): 72–82. http://dx.doi.org/10.1094/mpmi-09-16-0190-r.

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The main pathogenicity factor of Streptomyces species associated with the potato common scab disease is a nitrated diketopiperazine called thaxtomin A (ThxA). In Streptomyces scabiei (syn. S. scabies), which is thought to be the most ancient pathogenic Streptomyces species, the ThxA biosynthetic cluster is located within a mobile genomic island called the toxicogenic region (TR). Three attachment (att) sites further separate TR into two subregions (TR1 and TR2). TR1 contains the ThxA biosynthetic cluster and is conserved among several pathogenic Streptomyces species. However, TR2, an integrati
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14

Gregor, A. K., B. Klubek, and E. C. Varsa. "Identification and use of actinomycetes for enhanced nodulation of soybean co-inoculated with Bradyrhizobium japonicum." Canadian Journal of Microbiology 49, no. 8 (2003): 483–91. http://dx.doi.org/10.1139/w03-061.

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The utilization of actinomycetes as potential soybean (Glycine max (L.)) co-inoculants was evaluated. Soil samples from Carbondale and Belleville, Ill., were used to inoculate pre-germinated soybean plants to determine antibiotic sensitivity in the native Bradyrhizobium japonicum population. Sensitivity was in the order kanamycin > tetracycline > oxytetracycline > rifampicin > neomycin. Antagonism by five actinomycete cultures toward seven test strains of B. japonicum was also assessed. The ranking average inhibition (across all seven B. japonicum strains) by these actino mycetes w
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15

USHA, RAJAMANICKAM, KRISHNASWAMI KANJANA MALA, CHIDAMBARAM KULANDAISAMY VENIL, and MUTHUSAMY PALANISWAMY. "Screening of Actinomycetes from Mangrove Ecosystem for L-asparaginase Activity and Optimization by Response Surface Methodology." Polish Journal of Microbiology 60, no. 3 (2011): 213–21. http://dx.doi.org/10.33073/pjm-2011-030.

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Marine actinomycetes were isolated from sediment samples collected from Pitchavaram mangrove ecosystem situated along the southeast coast of India. Maximum actinomycete population was noted in rhizosphere region. About 38% of the isolates produced L-asparaginase. One potential strain KUA106 produced higher level of enzyme using tryptone glucose yeast extract medium. Based on the studied phenotypic characteristics, strain KUA106 was identified as Streptomyces parvulus KUA106. The optimization method that combines the Plackett-Burman design, a factorial design and the response surface method, wh
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16

Hamid, Mohamed E., Adil Mahgoub, Abdulrhman J. O. Babiker, et al. "Isolation and Identification of Streptomyces spp. from Desert and Savanna Soils in Sudan." International Journal of Environmental Research and Public Health 17, no. 23 (2020): 8749. http://dx.doi.org/10.3390/ijerph17238749.

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The purpose of this study was to investigate streptomycete populations in desert and savanna ecozones in Sudan and to identify species based on 16S rRNA gene sequences. A total of 49 different Streptomyces phenotypes (22 from sites representing the desert and semi-desert ecozone; 27 representing the savanna ecozone) have been included in the study. The isolates were characterized phenotypically and confirmed using 16S rRNA gene sequence analysis. The two ecozones showed both similarities and uniqueness in the types of isolates. The shared species were in cluster 1 (Streptomyces (S.) werraensis
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17

Wanner, Leslie A. "A Survey of Genetic Variation in Streptomyces Isolates Causing Potato Common Scab in the United States." Phytopathology® 96, no. 12 (2006): 1363–71. http://dx.doi.org/10.1094/phyto-96-1363.

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Common scab is a serious disease of potatoes and other root and tuber crops, affecting crop quality and market value. The disease is caused by gram positive soil bacteria in the genus Streptomyces. Disease incidence and severity vary in different locations and years; this is due in part to variation in the environment (weather) and genetic variation in potato cultivars. Little information is available on the contribution of genetic variation by the pathogen. To examine genetic diversity in different locations within the United States, streptomycetes were isolated from lesions on field-grown po
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18

Hernandez-Pérez, G., G. Goma, and J. L. Rols. "Enhanced degradation of lignosulfonated compounds by Streptomyces viridosporus." Water Science and Technology 38, no. 4-5 (1998): 289–97. http://dx.doi.org/10.2166/wst.1998.0647.

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Degradation of lignosulfonated compounds by S. viridosporus strain T7A on nutritive or mineral culture media was quantified. Two lignosulfonated compounds, differing in their molecular mass distributions and chemical structures, were used: lignosulfonate (1–80 kDa) and vanisperse, a sulfonated oxylignin (1–20 kDa). The use of nutritive culture medium (containing Biosoyase) enhanced both the growth of S. viridosporus and production of lignin peroxidase (LiP) leading to enhanced lignocellulose degradation, but no lignosulfonated compound degradation was observed. A fraction of these compounds un
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19

Govind, Govind Gulabrao Dhage, R. N. Ganbas Ravindra, and A. M. Garode Anil. "A review on Industrially important metabolite from Actinomycetes." International Journal of Applied and Advanced Biology (IJAAB) 2, no. 1 (2023): 07–17. http://dx.doi.org/10.60013/ijaab.v2i1.89.

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Microbial biotic compounds such as enzymes, pigments, antibiotics, and antimicrobial chemicals play an important function as metabolites, resulting in a wide range of applications and uses in a variety of sectors. Nowadays, the entire world is migrating away from synthetic and chemical products in favor of natural ones. Consistent study for novel microbial metabolites that could be employed in industrial processes, thereby driving profit growth in a variety of businesses. Actinomycetes are a major microbial population found in soil, plant tissues, fresh water, and marine settings. Many useful
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20

Newitt, Jake, Samuel Prudence, Matthew Hutchings, and Sarah Worsley. "Biocontrol of Cereal Crop Diseases Using Streptomycetes." Pathogens 8, no. 2 (2019): 78. http://dx.doi.org/10.3390/pathogens8020078.

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A growing world population and an increasing demand for greater food production requires that crop losses caused by pests and diseases are dramatically reduced. Concurrently, sustainability targets mean that alternatives to chemical pesticides are becoming increasingly desirable. Bacteria in the plant root microbiome can protect their plant host against pests and pathogenic infection. In particular, Streptomyces species are well-known to produce a range of secondary metabolites that can inhibit the growth of phytopathogens. Streptomyces are abundant in soils and are also enriched in the root m
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21

Kinkel, Linda L., John H. Bowers, Kyoko Shimizu, Eric C. Neeno-Eckwall, and Janet L. Schottel. "Quantitative relationships among thaxtomin A production, potato scab severity, and fatty acid composition in Streptomyces." Canadian Journal of Microbiology 44, no. 8 (1998): 768–76. http://dx.doi.org/10.1139/w98-061.

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Thaxtomin A production in culture, potato common scab severity (percentage of tuber surface infected or number of lesions per tuber), and fatty acid profiles were determined for 78 Streptomyces isolates. Only pathogenic Streptomyces spp. (n = 17) produced thaxtomin A in culture. Thaxtomin A production in culture (µg/mL) was significantly positively correlated with the percentage of tuber surface infected (R = 0.60; p = 0.017) but not with the number of lesions per tuber (R = 0.37; p = 0.17). An increase of 1 µg/mL in thaxtomin A production corresponded to an 11% increase in disease severity (p
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22

Milus, Eugene A., and Craig S. Rothrock. "Rhizosphere colonization of wheat by selected soil bacteria over diverse environments." Canadian Journal of Microbiology 39, no. 3 (1993): 335–41. http://dx.doi.org/10.1139/m93-047.

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The ability to colonize the rhizosphere is essential for bacteria to function as biological control agents for soil-borne plant pathogens. Eight bacterial strains reported to colonize wheat roots, inhibit root pathogens, and (or) improve wheat growth and yield were applied to wheat seeds that were planted in fumigated and nonfumigated soil in the 1990 and 1991 growing seasons at two locations in Arkansas. Rhizosphere population sizes were highly correlated with population sizes on seeds. Bacillus subtilis strain D-39Sr colonized roots as well in nonfumigated as in fumigated soil, and the other
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23

Lukyanchuk, V. V., S. L. Golembiovska, and L. V. Polishchuk. "Inheritance stability of hybrid plasmid TrS16 in transformant under long-term culture conditions without supporting selection." Faktori eksperimental'noi evolucii organizmiv 25 (August 30, 2019): 272–76. http://dx.doi.org/10.7124/feeo.v25.1176.

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Aim. The aim is to define the stability of inheritance the streptomycete recombinant plasmid TrS16 (8.4 kb) by TрS16 – 2 transformant cells of after the long-term storage without a purposeful selection (thiostrepton). Methods. Applied microbiological, genetic and physical-chemical methods. The recombinant plasmid of TrS16 was constructed on a base of the hybrid plasmid рАХ5а. The plasmidless variant of strain of Streptomyces globisporus 1912 was used as a recipient for the plasmid TrS16. Results. It is set that 5 % cells of transformant TрS16 – 2 population saved thiostrepton resistance after
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24

Wang, Zemin, Don L. Crawford, Anthony L. Pometto III, and Fatemeh Rafii. "Survival and effects of wild-type, mutant, and recombinant Streptomyces in a soil ecosystem." Canadian Journal of Microbiology 35, no. 5 (1989): 535–43. http://dx.doi.org/10.1139/m89-085.

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In a laboratory simulation, selected wild-type, mutant, and recombinant Streptomyces were released into a silt loam soil. Strains included genetically enhanced lignin decomposers and those expressing recombinant plasmids. Their survival and effects on soil organic carbon mineralization were monitored in sterile and nonsterile soil, with and without lignocellulose supplementation. Survival was followed by viable plate counts on selective media. CO2 evolution was monitored in respiration cabinets. All strains, whether released as spores or mycelia, survived in nonsterile soil for up to 30 days.
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25

Prastiti, Rachmanita D., Arga D. Indrawan, Penta Suryaminarsih, Tri Mujoko, and Bakti W. Widjajani. "Survivability and Benefit Evaluation of Streptomyces sp. and Trichoderma sp. as Active Ingredients of Biopesticides and Soil Fertility Enhancer in Shallot Fields at Wates Village Tulungagung." IOP Conference Series: Earth and Environmental Science 1131, no. 1 (2023): 012011. http://dx.doi.org/10.1088/1755-1315/1131/1/012011.

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Abstract The purpose of research on the Farmer Empowerment Program in Wates Village is to increase knowledge in the field of sustainable agriculture and transfer of technology for the application of biopesticides containing the Biological Control Agent (APH) Streptomyces sp., Trichoderma sp. combined with shallot cultivation technology in the village of Wates Tulungagung. Innovative methods and Participatory Action Research (PAR) are used to encourage transformative action, by involving community members as PAR implementers in describing the types of plants being treated and applying them duri
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26

Madheslu, Manikandan, Swapna Mani, Duraimurugan Kasiviswanathan, and Solai Ramatchandirane Prabagaran. "ANTAGONISTIC ACTINOBACTERIAL DIVERSITY OF MARINE SEDIMENTS COLLECTED FROM COASTAL TAMIL NADU." Asian Journal of Pharmaceutical and Clinical Research 12, no. 1 (2019): 478. http://dx.doi.org/10.22159/ajpcr.2018.v12i1.29660.

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Objective: The main objective of the current investigation is to identify the antagonistic potential of marine sediment actinobacterial population collected from coastal regions of Tamil Nadu and Kerala.Methods: A total of 60 marine sediment samples were collected from 27 locations of coastal Tamil Nadu and Kerala. A total of 85 morphologically distinct actinobacterial strains were isolated and taxonomically characterized. The strains were screened for its antagonistic potential against selected bacteria strains, and the obtained results were statistically analyzed. Further, the strains excell
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27

Madheslu, Manikandan, Swapna Mani, Duraimurugan Kasiviswanathan, and Solai Ramatchandirane Prabagaran. "ANTAGONISTIC ACTINOBACTERIAL DIVERSITY OF MARINE SEDIMENTS COLLECTED FROM COASTAL TAMIL NADU." Asian Journal of Pharmaceutical and Clinical Research 12, no. 1 (2019): 478. http://dx.doi.org/10.22159/ajpcr.2019.v12i1.29660.

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Objective: The main objective of the current investigation is to identify the antagonistic potential of marine sediment actinobacterial population collected from coastal regions of Tamil Nadu and Kerala.Methods: A total of 60 marine sediment samples were collected from 27 locations of coastal Tamil Nadu and Kerala. A total of 85 morphologically distinct actinobacterial strains were isolated and taxonomically characterized. The strains were screened for its antagonistic potential against selected bacteria strains, and the obtained results were statistically analyzed. Further, the strains excell
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28

Gao, Hong, Mei Liu, Ying Zhuo, et al. "Assessing the Potential of an Induced-Mutation Strategy for Avermectin Overproducers." Applied and Environmental Microbiology 76, no. 13 (2010): 4583–86. http://dx.doi.org/10.1128/aem.01682-09.

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ABSTRACT Mutant libraries of avermectin-producing Streptomyces avermitilis strains were constructed by different mutagenesis strategies. A metric was applied to assess the mutation spectrum by calculating the distribution of average phenotypic distance of each population. The results showed for the first time that a microgravity environment could introduce larger phenotype distribution and diversity than UV and N-methyl-N-nitro-N-nitrosoguanidine (NTG) could.
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29

Klein, Eyal, Maya Ofek, Jaacov Katan, Dror Minz, and Abraham Gamliel. "Soil Suppressiveness to Fusarium Disease: Shifts in Root Microbiome Associated with Reduction of Pathogen Root Colonization." Phytopathology® 103, no. 1 (2013): 23–33. http://dx.doi.org/10.1094/phyto-12-11-0349.

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Soil suppressiveness to Fusarium disease was induced by incubating sandy soil with debris of wild rocket (WR; Diplotaxis tenuifolia) under field conditions. We studied microbial dynamics in the roots of cucumber seedlings following transplantation into WR-amended or nonamended soil, as influenced by inoculation with Fusarium oxysporum f. sp. radicis-cucumerinum. Disease symptoms initiated in nonamended soil 6 days after inoculation, compared with 14 days in WR-amended soil. Root infection by F. oxysporum f. sp. radicis-cucumerinum was quantified using real-time polymerase chain reaction (PCR).
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30

Guan, Tony. "Metallotolerance of soil bacteria Streptomyces lusitanus found in vermicompost." Young Scientist 4, no. 1 (2025): 99–102. https://doi.org/10.5281/zenodo.14739822.

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Vermicompost is a commonly used compost soil to improve soil quality and provide fertilization for plants in agriculture because of its abundance of nutrition and diverse population of fungi and bacteria. This paper attempts to isolate bacteria from vermicompost obtained from a vermicompost box. A soil water extract-based medium and a selective medium added with Cadmium were used to perform bacteria inoculation. One bacterium was successfully isolated and purified. The purified bacterium was subjected to 16s rDNA PCR amplification and then DNA sequencing. The bacteria isolate was found to be S
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31

Sharif, Muhammad Anees, Faiz Ahmed Raza, and Imran Sajid. "Evaluation of Larvicidal Potential of Streptomyces extracts against Aedes Aegypti (Diptera: Culicidae)." JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS 5, no. 3 (2024): 189–203. https://doi.org/10.52700/jmmg.v5i3.182.

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Chemical pesticides are the main way to control mosquito population, but they are toxic to non-target organisms and have limited effectiveness against resistant mosquitoes. Actinobacterial metabolites are highly toxic, and their potential can be explored for alternative environment friendly insecticides. The current study evaluated methanolic crude extracts of 49 actinobacteria strains isolated from the Cholistan desert, Pakistan, for their larvicidal potential against fourth instar Aedes aegypti larvae. The extracts of five Streptomyces strains, including CD4-2, CD13-1, CD-1-1, CD13-6, and CD
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32

Wang, Ran, Yong Fang, Wunan Che, Qinghe Zhang, Jinda Wang, and Chen Luo. "Metabolic Resistance in Abamectin-Resistant Bemisia tabaci Mediterranean from Northern China." Toxins 14, no. 7 (2022): 424. http://dx.doi.org/10.3390/toxins14070424.

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Abamectin, produced by the soil-dwelling actinomycete Streptomyces avermitilis, belongs to the macrocyclic lactones class of pesticides, has nematocidal, acaricidal, and insecticidal activity, and is highly effective when used against targeted species. Bemisia tabaci, the tobacco whitefly, is a highly destructive insect to agricultural production worldwide, and various insecticide-resistant strains have been identified in China. Here, we monitored levels of resistance to abamectin in twelve field-collected B. tabaci populations from northern China, and confirmed that, compared with the lab ref
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33

Özyılmaz, Duygu Seren, and Muhammed Hasan Akyıl. "Use of Streptomyces species as a Biological Agent Against Plant ‎Pathogens." Journal of Molecular Virology and Immunology 2, no. 4 (2021): 151–58. https://doi.org/10.46683/jmvi.2021.42.

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<strong>Abstract</strong> Agricultural activities make significant contributions to the national economies around the world. Factors such as rapid population growth and unplanned urbanization cause a decrease in agricultural lands and agricultural crop production over the years with the effect of abiotic and biotic factors. Many studies have been carried out to eliminate factors that reduce crop productivity. Fungal pathogens, one of the leading biotic factors, cause plant diseases and reduce the production efficiency of crops.&nbsp;This study&nbsp;aimed to isolate&nbsp;<em>Streptomyces</em>&n
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RETNOWATI, YULIANA, LANGKAH SEMBIRING, SUKARTI MOELJOPAWIRO, TJUT SUGANDAWATY DJOHAN, and ENDANG SUTARININGSIH SOETARTO. "Diversity of antibiotic-producing Actinomycetes in mangrove forest of Torosiaje, Gorontalo, Indonesia." Biodiversitas Journal of Biological Diversity 18, no. 4 (2017): 1453–61. http://dx.doi.org/10.13057/biodiv/d180421.

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Retnowati Y, Sembiring L, Moeljopawiro S, Djohan TS, Soetarto ES. 2017. Diversity of antibiotic-producing actinomycetes in mangrove forest of Torosiaje, Gorontalo, Indonesia. Biodiversitas 18: 1453-1461. Actinomycetes for antibiotic production have been studied at various extreme environments. Mangrove forest of Torosiaje in Gorontalo Province, Indonesia has unique geomorphological conditions where the forest is surrounded by karst ecosystem consisting of fringe and overwash types. Therefore, the objective of this study was to analyze the distribution and diversity of antibiotic-producing Acti
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Sabaou, Nasserdine, and Nicole Bounaga. "Actinomycètes parasites de champignons : étude des espèces, spécificité de l'action parasitaire au genre Fusarium et antagonisme dans le sol envers Fusarium oxysporum f.sp. albedinis (Killian et Maire) Gordon." Canadian Journal of Microbiology 33, no. 5 (1987): 445–51. http://dx.doi.org/10.1139/m87-074.

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A study of two actinomycetes which are parasitic on fungi confirmed that one strain belongs to Nocardiopsis dassonvillei and the other to Streptomyces alni. Although both belong to different genera, they are physiologically related. A study of their parasitic action on numerous species in different genera of fungi revealed a specific mode of action on the genus Fusarium. In an autoclaved palm-grove soil, both actinomycetes strongly reduced propagule populations of Fusarium oxysporum f. sp. albedinis, a pathogen of date palm. This action was less pronounced in the same nonautoclaved soil, where
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Schlatter, Daniel C., Deborah A. Samac, Mesfin Tesfaye, and Linda L. Kinkel. "Rapid and Specific Method for Evaluating Streptomyces Competitive Dynamics in Complex Soil Communities." Applied and Environmental Microbiology 76, no. 6 (2010): 2009–12. http://dx.doi.org/10.1128/aem.02320-09.

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ABSTRACT Quantifying target microbial populations in complex communities remains a barrier to studying species interactions in soil environments. Quantitative PCR (qPCR) assays were developed for quantifying pathogenic Streptomyces scabiei and antibiotic-producing Streptomyces lavendulae strains in complex soil communities. This assay will be useful for evaluating the competitive dynamics of streptomycetes in soil.
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Thuy Hoai, Pham Thi, Ton That Huu Dat, Tran Thi Hong, Nguyen Thi Kim Cuc, Tran Dinh Man, and Pham Viet Cuong. "ISOLATION AND SELECTION OF INDIGENOUS ANTIFUNGAL MICROORGANISMS AGAINST PATHOGENIC FUNGI OF PEPPER PLANT IN TAY NGUYEN." Vietnam Journal of Biotechnology 16, no. 2 (2018): 385–92. http://dx.doi.org/10.15625/1811-4989/16/2/13452.

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The pathogenic fungi often cause huge impacts on agricultural crops, and occupy over 80% of plant diseases. Fusarium oxysporum and Rhizoctonia solani are fungal pathogens that can lead to rapid development of plant diseases on important crops in Tay Nguyen (e.g., pepper, coffee, rubber, cashew). Therefore, the study of microorganisms with bioactivity against these pathogens is essential to control plant diseases. In this study, we isolated microorganisms from rhizospheres of pepper in Tay Nguyen and screened beneficial microbes against two pathogenic fungi using agar well diffusion assay. Obta
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Joshi, Namrata, and Preeti Mahawar. "Optimization Of Cellulase Production By Various Species Isolated From Cellulose Rich Sites." International Journal of Pharma and Bio Sciences 12, no. 1 (2022): 206–13. http://dx.doi.org/10.22376/ijpbs/lpr.2022.12.1.l206-213.

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Cellulase is group of enzymes responsible for degradation of cellulose, a plant polymer. Cellulase has vast applications in various industries and hence continuous research is going on for better source of cellulase. Among all the different types of sources, microorganisms are found to be a prominent source of enzymes. As microorganisms can be easily isolated, grown and maintained for longer duration, making them potential enzyme producers. Industrial scale production through various types of fermentation enables large scale production. These microorganisms can be easily isolated from garden a
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Wichatham, Kawinthip, Pitchaya Piyaviriyakul, Narin Boontanon, Nawatch Surinkul, and Suwanna Kitpati Boontanon. "Potential Bacteria Isolated from Contaminated Sites as Bio-degraders of Various Types of Plastic." International Journal of Environmental Science and Development 14, no. 6 (2023): 348–54. http://dx.doi.org/10.18178/ijesd.2023.14.6.1454.

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The growing demand for plastics and their improper disposal have resulted in a significant environmental pollution problem. While various management strategies have been employed to tackle this issue, the persistence of plastic pollutants in the environment is still a major concern. Therefore, exploring and developing sustainable and environmentally safe techniques, such as biodegradation using potential bacteria, can help mitigate plastic pollution and provide a viable solution. The purpose of this study was to isolate and identify potential bacteria for degrading plastics from six soil sampl
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da Silva Pinto, Lívia Freitas, Daniel dos Santos Caldas, Maria Clara Coelho Prazeres, et al. "Bioprospecting of Actinobacteria in Rhizosphere of Amazonic Soils and Evaluation of Biotechnological Potential for the Production of Antimicrobials - Igarapé-Açu, Pará, Brazil." International Journal of Current Microbiology and Applied Sciences 11, no. 3 (2022): 201–10. http://dx.doi.org/10.20546/ijcmas.2022.1103.024.

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Actinobacteria are important components of the microbial population in most soils and are widely distributed and abundant, especially in the rhizosphere. The phylum Actinobacteria, particularly the genus Streptomyces, is recognized for its potential to produce a vast quantity of secondary metabolites, among which there are bioactive compounds used as antibiotics. The present study sought to isolate and identify actinobacteria from secondary forest rhizosphere in the region of Igarapé-Açu and to evaluate the biotechnological potential of these strains for the production of antimicrobials. Thirt
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Farda, Beatrice, Rihab Djebaili, Ilaria Vaccarelli, Maddalena Del Gallo, and Marika Pellegrini. "Actinomycetes from Caves: An Overview of Their Diversity, Biotechnological Properties, and Insights for Their Use in Soil Environments." Microorganisms 10, no. 2 (2022): 453. http://dx.doi.org/10.3390/microorganisms10020453.

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The environmental conditions of caves shape microbiota. Within caves’ microbial communities, actinomycetes are among the most abundant bacteria. Cave actinomycetes have gained increasing attention during the last decades due to novel bioactive compounds with antibacterial, antioxidant and anticancer activities. However, their potential role in soil environments is still unknown. This review summarises the literature dealing with actinomycetes from caves, underlining for the first time their potential roles in soil environments. We provide an overview of their diversity and biotechnological pro
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Conn, Vanessa M., and Christopher M. M. Franco. "Analysis of the Endophytic Actinobacterial Population in the Roots of Wheat (Triticum aestivum L.) by Terminal Restriction Fragment Length Polymorphism and Sequencing of 16S rRNA Clones." Applied and Environmental Microbiology 70, no. 3 (2004): 1787–94. http://dx.doi.org/10.1128/aem.70.3.1787-1794.2004.

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ABSTRACT The endophytic actinobacterial population in the roots of wheat grown in three different soils obtained from the southeast part of South Australia was investigated by terminal restriction fragment length polymorphism (T-RFLP) analysis of the amplified 16S rRNA genes. A new, validated approach was applied to the T-RFLP analysis in order to estimate, to the genus level, the actinobacterial population that was identified. Actinobacterium-biased primers were used together with three restriction enzymes to obtain terminal restriction fragments (TRFs). The TRFs were matched to bacterial gen
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Sturz, A. V., R. D. Peters, M. R. Carter, J. B. Sanderson, B. G. Matheson, and B. R. Christie. "Variation in antibiosis ability, against potato pathogens, of bacterial communities recovered from the endo- and exoroots of potato crops produced under conventional versus minimum tillage systems." Canadian Journal of Microbiology 51, no. 8 (2005): 643–54. http://dx.doi.org/10.1139/w05-041.

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The culturable component of bacterial communities found in the endoroot and associated exoroot (root zone soil) was examined in potatoes (Solanum tuberosum L.) grown under either conventional or minimum tillage systems. Bacterial species – abundance relationships were determined and in vitro antibiosis ability investigated to discover whether tillage practice or bacteria source (endo- or exoroot) influenced bacterial community structure and functional versatility. Antibiosis abilities against Phytophthora erythroseptica Pethyb. (causal agent of pink rot of potatoes), Streptomyces scabies (Thax
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Azmin, Nur Raihan Aqilah Mohammad, Nurul ‘Izzah Mohd Sarmin, Hasnah Begum Said Gulam Khan, and Mukarramah Zainal. "Identification of Endophytic Actinomycetes Isolated From Hopea ferrea and Its Antibacterial Activity Against Cariogenic Bacterium." Malaysian Applied Biology 53, no. 2 (2024): 37–46. http://dx.doi.org/10.55230/mabjournal.v53i2.2756.

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Dental caries affects around 36% of the world's population and results in the loss of primary teeth in about 530 million youngsters. It is described as the loss of the enamel layer of the tooth by acids generated by the activity of cariogenic bacterium such as Streptococcus mutans on carbohydrates. This study aims to identify bioactive endophytic actinomycetes as an antibacterial agent against S. mutans DSM 20523 and to identify the bioactive endophytic actinomycetes using the 16S rRNA sequencing approach. To determine the percentage zone of inhibition, three endophytes at 10-day-old culture h
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Ali, Alimuddin, and Herlina Rante. "Karakterisasi Mikrobia Rizosfer asal Tanaman Ginseng Jawa (Talinum triangulare) berdasarkan Gen Ribosomal 16S rRNA dan 18S rRNA." JURNAL BIOLOGI PAPUA 3, no. 2 (2018): 74–81. http://dx.doi.org/10.31957/jbp.552.

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The rhizosphere is a biologically active zone of the soil around plant roots that contains soil-borne microbes including bacteria and fungi. The microbes were isolated from rhizosphere soil roots of Java ginseng. The population of microbes was estimated by plate count method. The isolates were identified based on a great variety of morphological, and cultural characteristics. The total of rhizosphere soil microbe population were 20.91(106 cfu.g−1soils) and showed that 12 isolates of bacteria, 15 isolates of actinomycetes, and 10 isolates of fungi which were found in all of soil samples. The mo
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Kortemaa, Hanna, Kielo Haahtela, and Aino Smolander. "Effect of soil-spraying time on root-colonization ability of antagonistic Streptomyces griseoviridis." Agricultural and Food Science 6, no. 4 (1997): 341–48. http://dx.doi.org/10.23986/afsci.72797.

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The root-colonization ability of Streptomyces griseoviridis Anderson et al. was tested on turnip rape (Brassica rapa subsp. oleifera DC.) and carrot (Daucus carota L.) by the sand-tube method. Nonsterile sand was sprayed with a microbial suspension immediately or 7 days after the seed had been sown. Results expressed as population frequencies and densities indicated that S. griseoviridis effectively colonizes the rhizosphere when the microbe is applied immediately after sowing but less effectively when it is applied 7 days later. Detection values of S. griseoviridis were higher for turnip rape
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Zhang, Jianfeng, Hengfei Zhang, Shouyang Luo, et al. "Analysis and Functional Prediction of Core Bacteria in the Arabidopsis Rhizosphere Microbiome under Drought Stress." Microorganisms 12, no. 4 (2024): 790. http://dx.doi.org/10.3390/microorganisms12040790.

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The effects of global warming, population growth, and economic development are increasing the frequency of extreme weather events, such as drought. Among abiotic stresses, drought has the greatest impact on soil biological activity and crop yields. The rhizosphere microbiota, which represents a second gene pool for plants, may help alleviate the effects of drought on crops. In order to investigate the structure and diversity of the bacterial communities on drought stress, this study analyzed the differences in the bacterial communities by high-throughput sequencing and bioinformatical analyses
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Zhou, Xingxing, Bo Zhang, Qingzhu Meng, and Lingmei Li. "Effects of Graphene Oxide on Endophytic Bacteria Population Characteristics in Plants from Soils Contaminated by Polycyclic Aromatic Hydrocarbons." Molecules 29, no. 10 (2024): 2342. http://dx.doi.org/10.3390/molecules29102342.

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Environmental pollution stands as one of the significant global challenges we face today. Polycyclic aromatic hydrocarbons (PAHs), a class of stubborn organic pollutants, have long been a focal point of bioremediation research. This study aims to explore the impact and mechanisms of graphene oxide (GO) on the phytoremediation effectiveness of PAHs. The results underscore the significant efficacy of GO in accelerating the degradation of PAHs. Additionally, the introduction of GO altered the diversity and community structure of endophytic bacteria within the roots, particularly those genera with
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Arseneault, Tanya, Claudia Goyer, and Martin Filion. "Biocontrol of Potato Common Scab is Associated with High Pseudomonas fluorescens LBUM223 Populations and Phenazine-1-Carboxylic Acid Biosynthetic Transcript Accumulation in the Potato Geocaulosphere." Phytopathology® 106, no. 9 (2016): 963–70. http://dx.doi.org/10.1094/phyto-01-16-0019-r.

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Pseudomonads are often used as biocontrol agents because they display a broad range of mechanisms to control diseases. Common scab of potato, caused by Streptomyces scabies, was previously reported to be controlled by Pseudomonas fluorescens LBUM223 through phenazine-1-carboxylic acid (PCA) production. In this study, we aimed at characterizing the population dynamics of LBUM223 and the expression of phzC, a key gene involved in the biosynthesis of PCA, in the rhizosphere and geocaulosphere of potato plants grown under controlled and field conditions. Results obtained from controlled experiment
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Rebai, Hadjer, Essam Nageh Sholkamy, Reem Mohammed Alharbi, et al. "Streptomyces sp. Strain SRH22: A Potential Bioremediation Agent for Glyphosate-Contaminated Agricultural Soils." Environment and Natural Resources Journal 21, no. 6 (2023): 1–12. http://dx.doi.org/10.32526/ennrj/21/20230181.

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Glyphosate, also known as N-phosphonomethylglycine, is the herbicide that is widely used across the globe. As there are concerns over its potential toxicity to non-target soil species, there is a growing interest in identifying glyphosate-degrading microorganisms in soil. Biodegradation, by actinobacteria, is a very promising approach to eliminate this pesticide from contaminated environments. The present work isolated and identified actinobacteria capable of degrading glyphosate from Saharan agriculture, as well as determined how the application of this herbicide affects the abundance of acti
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