Academic literature on the topic 'Strong biofilm-forming bacteria'
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Journal articles on the topic "Strong biofilm-forming bacteria"
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Otokunefor, Kome, Deborah Melex, and Gideon Abu. "Biofilm Forming Potential of Escherichia coli from Various Sources." Journal of Life and Bio Sciences Research 1, no. 2 (2020): 26–29. http://dx.doi.org/10.38094/jlbsr1210.
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Majority of bacterial communities exist as biofilms and these contribute to the survival of the bacteria. Biofilm development has been associated with protection from adverse environmental conditions and resistance to harmful agents. Generally, however data on biofilm-forming potential of bacteria in Nigeria is sparse. This study was therefore aimed at analyzing variations in biofilm-forming potential of Escherichia coli from various sources in Port Harcourt, Nigeria. Previously characterized clinical (30) and non-clinical (30) E. coli isolates were assessed for their biofilm-forming potential using the Congo Red agar method and variations in this potential determined as weak, moderate or strong. Majority of isolates (67%) had the potential to form biofilms but only 40% of isolates exhibiting biofilm-forming potential were from clinical sources. Isolates exhibited variable degrees of biofilm-forming potential, with only non-clinical isolates exhibiting strong potential. Majority of both clinical and non-clinical isolates (68.7% and 88% respectively) exhibited moderate biofilm-forming potential. The higher occurrence of E. coli exhibiting biofilm-forming potential among non-clinical isolates possibly reflects the essential role biofilms play in the survival of bacteria in nature, but not in infection cases. This study reports on a high level association between the isolates and biofilm production and highlights differences in the abilities of biofilm production between clinical and non-clinical isolates.
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LIU, NANCY T., ALAN M. LEFCOURT, XIANGWU NOU, DANIEL R. SHELTON, GUODONG ZHANG, and Y. MARTIN LO. "Native Microflora in Fresh-Cut Produce Processing Plants and Their Potentials for Biofilm Formation." Journal of Food Protection 76, no. 5 (2013): 827–32. http://dx.doi.org/10.4315/0362-028x.jfp-12-433.
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Representative food contact and nonfood contact surfaces in two mid-sized, fresh-cut processing facilities were sampled for microbiological analyses after routine daily sanitization. Mesophilic and psychrotrophic bacteria on the sampled surfaces were isolated by plating on nonselective bacterial media. Alternatively, bacteria were isolated after an incubation period that allowed the formation of heterogeneous biofilms on stainless steel beads. Of over 1,000 tested isolates, most were capable of forming biofilms, with approximately 30% being strong or moderate biofilm formers. Selected isolates (117) were subjected to species identification by using the Biolog Gen III microbial identification system. They distributed among 23 genera, which included soil bacteria, plant-related bacteria, coliforms, and opportunistic plant- or human-pathogenic bacteria. The most commonly identified bacteria species were Pseudomonas fluorescens, Rahnella aquatilis, and Ralstonia insidiosa. The high prevalence of R. insidiosa, a strong biofilm former, and P. fluorescens, a moderate biofilm former, suggests that they were established residents in the sampled plants. These results suggest that native microflora capable of forming biofilms are widely distributed in fresh-produce processing environments.
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Ngo, Thanh Phong, Huynh Lien Bui, Thi Binh Nguyen Pham, Van Tien Huynh, and The Vinh Bui. "Identifying biofilm forming bacteria in cow milk in Mekong Delta, Viet Nam." Can Tho University Journal of Science 15, no. 1 (2023): 84–90. http://dx.doi.org/10.22144/ctu.jen.2023.011.
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Bacterial biofilms are agglomeration of bacterial cells, stuck to the material surfaces of material in wet environments and formed by a self-produced matrix. The formation of bacterial biofilms is a great risk for the milk processing industry, as the survival of many bacterial species in cow milk may lead to many problems such as microbial spoilage, deterioration in quality, and consumer health risks. This study aimed to identify biofilm formation bacteria from cow milk. The experiment included isolation; biofilm forming assay in 96-well microtiter plates and the identification of microbial isolates using classical and molecular biological methods. A total of 14 bacterial isolates from 10 cow milk samples were evaluated for their biofilm formatting ability. Among them, four isolates were identified as moderate and strong biofilm producers. These four isolates belong to the genera Serratia and Aeromonas. Out of the 4 isolates, Serratia marcescens VL41 was classed as a strong biofilm producer while Aeromonas veronii ST15, Aeromonas sp. ST17, Serratia marcescens VL13 were classed as moderate biofilm producers respectively. The findings of this study suggest that it is necessary to discover the contamination causes and prevention of genera Serratia, and Aeromonas into cow milk.
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Ngongang, Dimitri Tchami, Ascension Maximilienne Nyegue, Del Florence Esther Ndedi, and Xavier François Etoa. "Effect of Four Essential Oils on Cells Release Membrane and Biofilm Formation of Clinical Bacterial Isolated From Oral Infection." Journal of Drug Delivery and Therapeutics 9, no. 6 (2019): 28–35. http://dx.doi.org/10.22270/jddt.v9i6.3600.
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Background: Bacterial biofilms forming are current resistant bacterial form to the treatment of oral diseases that colonizes in the gingival and sub-gingival regions of the mouth. The present study aims to screen the anti-biofilm potential and evaluate the effect of four essential oils on cells release membrane. Methods: Seven type isolate bacteria obtained during previous work were screen to select those who had ability to form biofilm using Congo Red Agar method, tube method and crystal violet method. The inhibitory parameter of biofilm forming was determine using microtiter plate method. The effect of essential oil on cell membrane release of each selected bacterial was put in evidence by measuring cellular material that absorb at 260 nm and 280 nm after 0 min, 30 min and 60 min of exposure and confirm by measuring DNA, RNA and proteins release by treated cells on extracellular medium using Nanodrop 1000 spectrophotometer. Results: The crystal violet method shows twelve (12) strong, five (05) moderate and five (05) weak biofilm forming bacteria. The anti-biofilm activity against the oral bacteria who shown that most of essentials oils have activity on different biofilm formation and the MICs ranged from 0.31 mg/mL to 1.25 mg/mL. Concentration of intracellular material released in extracellular medium ranged from 186,56 ± 2,35 ng/µL to 766,6 ± 2,84 ng/µL for DNA, 158,06 ± 1,87 ng/µL to 628,53 ± 2,05 ng/µL for RNA and 695,9 ± 2,11ng/µL to 1125,23 ± 2,15 ng/µL for proteins. Conclusion: This study demonstrates that the selected EOs have a significant anti-biofilm activity, acting on the cell surface and causing the disruption of the bacterial membrane. These EOs are interesting alternative to conventional antimicrobials for the control of oral microorganisms.
 Keywords: Anti-biofilm activity, Biofilm, oral diseases
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Petkova, Tsvetelina, Nikolina Rusenova, Svetla Danova, and Aneliya Milanova. "Effect of N-Acetyl-L-cysteine on Activity of Doxycycline against Biofilm-Forming Bacterial Strains." Antibiotics 12, no. 7 (2023): 1187. http://dx.doi.org/10.3390/antibiotics12071187.
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Biofilm-forming bacteria are associated with difficult-to-cure bacterial infections in veterinary patients. According to previous studies, N-acetyl-L-cysteine (NAC) showed an inhibitory effect on biofilm formation when it was applied in combination with beta-lactam antibiotics and fluoroquinolones. The lack of information about the effect of NAC on doxycycline activity against biofilm-forming strains was the reason for conducting this study. Staphylococcus aureus (S. aureus) ATCC 25923, Staphylococcus aureus O74, Escherichia coli (E. coli) ATCC 25922 and Pseudomonas aeruginosa (P. aeruginosa) ATCC 27853 were used to evaluate the activity of doxycycline with and without addition of NAC on planktonic bacteria and on biofilm formation. The minimum inhibitory concentrations (MICs) of doxycycline were not affected by NAC for Gram-negative strains and were found to be two times higher for the strains of S. aureus. The minimum biofilm inhibitory concentrations (MBICs) for Gram-negative bacteria (2 μg/mL for E. coli ATCC 25922 and 32 μg/mL for P. aeruginosa ATCC 27853), determined using a standard safranin colorimetric assay, were higher than the MICs (0.5 and 4 μg/mL, respectively). The data suggest that the combinations of doxycycline and NAC could stimulate the growth of planktonic cells of S. aureus and biofilm-forming E. coli ATCC 25922. NAC did not affect the strong inhibitory effect of doxycycline on the biofilm formation by the strains of S. aureus.
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Eid, Doaa, Ossama M. Sayed, Walaa G. Hozayen, and Ahmed F. Azmy. "Battling Biofilm Forming Nosocomial Pathogens Using Chitosan and Pluronic F127." Journal of Pure and Applied Microbiology 14, no. 3 (2020): 1893–903. http://dx.doi.org/10.22207/jpam.14.3.28.
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Biofilm represents a potential strut in bacterial treatment failure. It has a dual action; it affords microbial resistance against antibiotics and facilitate transmission of pathogenic bacteria. Nosocomial bacteria pose a serious problem in healthcare units; it prolongs patient hospital stay and increases the mortality rates beside other awful economical effect. This study was planned for targeting nosocomial bacterial biofilm using natural and biologically safe compounds like Chitosan and/or Pluronic F127. Ninety-five isolates were recovered from 107 nosocomial clinical samples. Different bacterial and fungal species were detected, from which Klebsiella pneumonia (23%), Pseudomonas aeruginosa (19%), Acinetobacter baumannii (18%) and E.coli (17%) were the predominate organisms. Pseudomonas aeruginosa, Acinetobacter baumanni and Klebsiella pneumonia were the abundant antibiotic resistant strains with multi-resistance pattern of 72%, 65% and 59%, respectively. A significant percentage of these isolates were strong biofilm forming. Herein, we investigate the effect of Chitosan and Pluronic F127 alone and in combination with each other against biofilm production. Chitosan show variable degree of biofilm inhibition, while Pluronic F127 was able to retard biofilm formation by 80% to 90% in most strain. There is no significant difference (P< 0.05) between Pluronic F127 alone and its effect in combination with Chitosan.
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A. Mahdi, Estabraq, Abdulwahid B. Al-Shaibani, and Nedhaal S. Zbar. "The impact of Glucose and Sodium Chloride on the Biofilm Formation of Pseudomonas aeruginosa & Staphylococcus aureus." Ibn AL- Haitham Journal For Pure and Applied Sciences 33, no. 4 (2020): 1–9. http://dx.doi.org/10.30526/33.4.2525.
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The aim of this research is to evaluate the effect of glucose and sodium chloride on biofilm formation by bacteria causing wound infection. For this purpose, 1% and 2% concentration of each of glucose and sodium chloride were used to test the biofilm formation potential of Staphylococcus aureus and Pseudomonas aeruginosa, which were the most common abundant bacteria that cause infection by biofilm. Each of the concentrations was kept in contact with the pathogenic bacteria for 24 hours. After the period of incubation, the concentration of 1% of glucose enhanced moderate biofilm formation capacity for (66% and 80%) on both bacteria respectively. The concentration of 2% glucose, on the other hand, led to a weak biofilm for 33% and 20% on both bacteria isolates respectively. In respect to the effect of sodium chloride, no isolate was able to form neither moderate nor strong biofilms. Nonetheless, all isolates succeeded in forming weak biofilms at 2% sodium chloride, while treatment with a concentration of 1% sodium chloride led to inhibited biofilm formation for 43% of isolates. Besides, Pseudomonas aeruginosa isolates were able to form moderate biofilms in the presence of 1% concentration of glucose, and weak producers in the presence of 2% glucose concentration. The isolates succeeded in forming strong biofilms at both 1% and 2% sodium chloride.
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Muras, Andrea, Ana Parga, Celia Mayer, and Ana Otero. "Use of Quorum Sensing Inhibition Strategies to Control Microfouling." Marine Drugs 19, no. 2 (2021): 74. http://dx.doi.org/10.3390/md19020074.
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Interfering with the quorum sensing bacterial communication systems has been proposed as a promising strategy to control bacterial biofilm formation, a key process in biofouling development. Appropriate in vitro biofilm-forming bacteria models are needed to establish screening methods for innovative anti-biofilm and anti-microfouling compounds. Four marine strains, two Pseudoalteromonas spp. and two Vibrio spp., were selected and studied with regard to their biofilm-forming capacity and sensitivity to quorum sensing (QS) inhibitors. Biofilm experiments were performed using two biofilm cultivation and quantification methods: the xCELLigence® system, which allows online monitoring of biofilm formation, and the active attachment model, which allows refreshment of the culture medium to obtain a strong biofilm that can be quantified with standard staining methods. Although all selected strains produced acyl-homoserine-lactone (AHL) QS signals, only the P. flavipulchra biofilm, measured with both quantification systems, was significantly reduced with the addition of the AHL-lactonase Aii20J without a significant effect on planktonic growth. Two-species biofilms containing P. flavipulchra were also affected by the addition of Aii20J, indicating an influence on the target bacterial strain as well as an indirect effect on the co-cultured bacterium. The use of xCELLigence® is proposed as a time-saving method to quantify biofilm formation and search for eco-friendly anti-microfouling compounds based on quorum sensing inhibition (QSI) strategies. The results obtained from these two in vitro biofilm formation methods revealed important differences in the response of biosensor bacteria to culture medium and conditions, indicating that several strains should be used simultaneously for screening purposes and the cultivation conditions should be carefully optimized for each specific purpose.
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Barak, Tamar, Eden Sharon, Doron Steinberg, Mark Feldman, Ronit Vogt Sionov, and Miriam Shalish. "Anti-Bacterial Effect of Cannabidiol against the Cariogenic Streptococcus mutans Bacterium: An In Vitro Study." International Journal of Molecular Sciences 23, no. 24 (2022): 15878. http://dx.doi.org/10.3390/ijms232415878.
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Dental caries is caused by biofilm-forming acidogenic bacteria, especially Streptococcus mutans, and is still one of the most prevalent human bacterial diseases. The potential use of cannabidiol (CBD) in anti-bacterial therapies has recently emerged. Here we have studied the anti-bacterial and anti-biofilm activity of CBD against S. mutans. We measured minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC). The bacterial growth and changes in pH values were measured in a kinetic study. The biofilm biomass was assessed by Crystal Violet staining and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) metabolic assay. Spinning Disk Confocal Microscopy (SDCM) was used to assess biofilm structure, bacterial viability and extracellular polysaccharide (EPS) production. CBD inhibited S. mutans planktonic growth and biofilm formation in a dose-dependent manner, with similar MIC and MBIC values (5 µg/mL). CBD prevented the bacteria-mediated reduction in pH values that correlated with bacterial growth inhibition. SDCM showed a decrease of 50-fold in live bacteria and EPS production. CBD significantly reduced the viability of preformed biofilms at 7.5 µg/mL with an 80 ± 3.1% reduction of metabolic activity. At concentrations above 20 µg/mL, there was almost no bacterial recovery in the CBD-treated preformed biofilms even 48 h after drug withdrawal. Notably, precoating of the culture plate surfaces with CBD prior to incubation with bacteria inhibited biofilm development. Additionally, CBD was found to induce membrane hyperpolarization in S. mutans. Thus, CBD affects multiple processes in S. mutans including its cariogenic properties. In conclusion, we show that CBD has a strong inhibitory effect against cariogenic bacteria, suggesting that it is a potential drug adjuvant for reducing oral pathogenic bacterial load as well as protecting against dental caries.
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Sornchuer, Phornphan, Kritsakorn Saninjuk, Parisa Prathaphan, Rattana Tiengtip, and Suphot Wattanaphansak. "Antimicrobial Susceptibility Profile and Whole-Genome Analysis of a Strong Biofilm-Forming Bacillus Sp. B87 Strain Isolated from Food." Microorganisms 10, no. 2 (2022): 252. http://dx.doi.org/10.3390/microorganisms10020252.
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Members of the Bacillus cereus group are considered to be foodborne pathogens commonly associated with diarrheal and emetic gastrointestinal syndromes. Biofilm formation is a major virulence determinant of various pathogenic bacteria, including the B. cereus strains, since it can protect the bacteria against antimicrobial agents and the host immune response. Moreover, a biofilm allows the exchange of genetic material, such as antimicrobial resistance genes, among the different bacterial strains inside the matrix. The aim of the current study was to genotypically and phenotypically characterize Bacillus sp. B87, a strain that was isolated from food and which exhibited strong biofilm-forming capacity. Based on the analysis of the phylogenetic relationship, the isolate was phylogenetically mapped close to Bacillus pacificus. Antimicrobial susceptibility testing revealed that the isolate was resistant to tetracycline and β-lactam antimicrobial agents, which corresponded with the genotypic characterization using the whole-genome analysis. The genome of Bacillus sp. B87 carried the three-component non-hemolytic enterotoxin (NHE), which is a type of enterotoxin that causes diarrheal symptoms. In addition, the genome also contained several genes that participate in biofilm formation, including the pelDEADAFG operon. These findings expand our understanding of antimicrobial resistance and virulence in Bacillus species based on the link between genotypic and phenotypic characterization.
Book chapters on the topic "Strong biofilm-forming bacteria"
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Permeh, Samanbar, Carla Reid, Mayrén Echeverría Boan, et al. "Microbiological Influenced Corrosion (MIC) in Florida Marine Environment: A Case Study." In Case Studies in Corrosion Failures. AMPP, Association for Materials Protection and Performance15835 Park Ten Place, Houston, TX 77084, 2025. https://doi.org/10.5006/37692-ch19.
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Abstract Microbiologically Influenced Corrosion (MIC) occurs in environments where microbial attachment and biofilm formation occurs. The microbial metabolic activities which cause MIC affect materials in a wide variety of industries. Although MIC has not traditionally been a major durability concern for Florida coastal and inland bridges, a recent finding by the Florida Department of Transportation (FDOT) of severe corrosion of steel bridge piles with strong evidence of microbial activity, has motivated the present study. As a preliminary research, identify the possible susceptibility of a case study marine bridge infrastructure to MIC is the main objective. This will be supported by determining the bacteria, nutrient levels, environmental conditions and other factors that could support MIC. A site visit to a bridge was carried out in 2016 and water samples (close to the site) at varying depths, as well as underwater pictures of the bridge steel piles were taken. The chemical composition including pH, total organic nitrogen, nitrate, phosphate, sulfate, chloride, ammonia, and microbiological content of the samples were determined. Sulfate Reducing Bacteria (SRB), Slime Forming Bacteria (SFB), Iron Reducing Bacteria (IRB), and Acid Producing Bacteria (APB) were found in water samples. The presence of carbon, sulfate, nitrogen, phosphorus, as well as Ca, K, Na, Mg in water samples of the case study could provide the necessary nutrient to support large bacteria colonization. Site visit results (water chemistry and microorganism content) were compared with database information of water management districts of Florida, in order to find similar conditions that could support MIC. As a result, many sites with similar characteristics as the case study were found that may support MIC.
Conference papers on the topic "Strong biofilm-forming bacteria"
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Permeh, Samanbar, Carla Reid, Mayrén Echeverría Boan, et al. "Microbiological Influenced Corrosion (MIC) in Florida Marine Environment: a Case Study." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09536.
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Abstract Microbiologically Influenced Corrosion (MIC) occurs in environments where microbial attachment and biofilm formation occurs. The microbial metabolic activities which cause MIC affect materials in a wide variety of industries. Although MIC has not traditionally been a major durability concern for Florida coastal and inland bridges, a recent finding by the Florida Department of Transportation (FDOT) of severe corrosion of steel bridge piles with strong evidence of microbial activity, has motivated the present study. As a preliminary research, identify the possible susceptibility of a case study marine bridge infrastructure to MIC is the main objective. This will be supported by determining the bacteria, nutrient levels, environmental conditions and other factors that could support MIC. A site visit to a bridge was carried out in 2016 and water samples (close to the site) at varying depths, as well as underwater pictures of the bridge steel piles were taken. The chemical composition including pH, total organic nitrogen, nitrate, phosphate, sulfate, chloride, ammonia and microbiological content of the samples were determined. Sulfate Reducing Bacteria (SRB), Slime Forming Bacteria (SFB), Iron Reducing Bacteria (IRB), and Acid Producing Bacteria (APB) were found in water samples. The presence of carbon, sulfate, nitrogen, phosphorus, as well as Ca, K, Na, Mg in water samples of the case study could provide the necessary nutrient to support large bacteria colonization. Site visit results (water chemistry and microorganism content) were compared with database information of water management districts of Florida, in order to find similar conditions that could support MIC. As a result, many sites with similar characteristics as the case study were found that may support MIC.
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G.O. Al-Ani, Amina. "The Effect of Cinnamon and Cardamom Oils On Some Virulence Factors of Pseudomonas aeruginosa." In XII. International Scientific Congress of Pure, Applied and Technological Sciences. Rimar Academy, 2024. https://doi.org/10.47832/minarcongress12-02.
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Globally, drug resistance by Pseudomonas aeruginosa is a major public health concern. It is important to look at different treatment methods and as a result, the approach taken in this work is to harness essential oils (EO) to combat the pathogenesis of bacterial diseases. Therefore, the aim of this study is to examine the effects of cardamom and cinnamon oils on P. aeruginosa biofilm formation, pyocyanin synthesis, and swarming motility. Each oil was used at four different concentrations (0.1, 0.2, 0.4, and 0.8) μl/ml to examine how it affects the formation of biofilms and the production of pyocyanin, while 0.2 μl/ml has been used for swarming motility .Initially, the isolate showed the ability for forming strong biofilms.When studying the influence of cardamom and cinnamon oils on the formation of biofilms, cinnamon oil was more effective at a 0.8 μl/ml concentration, while cardamom oil did not show any effect at all concentrations used. As for the effect of the oils used on the pyocyanin production, it showed that cinnamon oil had a greater effect at ( 0.4 , 0.8) μl/ml, where the isolate under study lost its ability to produce the pigment pyocyanin. The cardamom oil had the lowest pigment production at concentration 0.8 μl/ml compared to the control and other concentrations. Finally, the results of the effect of oils used at 0.2 μl/ml concentration on the swarm movement showed that cinnamon oil was more effective in inhibiting movement compared to cardamom oil, as the diameter of the growing colony in the presence of cardamom and cinnamon oils was 15 mm and 10 mm, respectively, compared to the control 20 mm on Swarm agar medium