Log in

Relevant bibliographies by topics / Strukturní protein / Dissertations / Theses

To see the other types of publications on this topic, follow the link: Strukturní protein.

Dissertations / Theses on the topic 'Strukturní protein'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 dissertations / theses for your research on the topic 'Strukturní protein.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.

1

Dressel, Frank. "Sequenz, Energie, Struktur - Untersuchungen zur Beziehung zwischen Primär- und Tertiärstruktur in globulären und Membran-Proteinen." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2008. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1222781322751-68621.

Full text

Abstract:

Proteine spielen auf der zellulären Ebene eines Organismus eine fundamentale Rolle. Sie sind quasi die „Maschinen“ der Zelle. Ihre Bedeutung wird nicht zuletzt in ihrem Namen deutlich, welcher 1838 erstmals von J. Berzelius verwendet wurde und „das Erste“, „das Wichtigste“ bedeutet. Proteine sind aus Aminosäuren aufgebaute Moleküle. Unter physiologischen Bedingungen besitzen sie eine definierte dreidimensionale Gestalt, welche für ihre biologische Funktion bestimmend ist. Es wird heutzutage davon ausgegangen, dass diese dreidimensionale, stabile Struktur von Proteinen eindeutig durch die Abfol

APA, Harvard, Vancouver, ISO, and other styles

2

Hyrš, Martin. "Predikce vlivu aminokyselinových mutací na sekundární strukturu proteinů." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2013. http://www.nusl.cz/ntk/nusl-236396.

Full text

Abstract:

In this thesis I investigate the effect of amino acid substitutions on secondary structure of proteins. I found that the secondary structure is relatively resistant to mutations, some regions hold the same secondary structure, even though their sequences are very different. Since this effect was observed also for random sequences, I conclude that it is a general property of the amino acid sequence. The particular elements of secondary structures are differentially sensitive to the changes caused by mutations. Protein's sensitivity to mutations depends on the composition of its secondary struct

APA, Harvard, Vancouver, ISO, and other styles

3

Tuček, Jaroslav. "Předpovídání struktury proteinů." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2009. http://www.nusl.cz/ntk/nusl-236732.

Full text

Abstract:

This work describes the three dimensional structure of protein molecules and biological databases used to store information about this structure or its hierarchical classification. Current methods of computational structure prediction are overviewed with an emphasis on comparative modeling. This particular method is also implemented in a proof-of-concept program and finally, the implementation is analysed.

APA, Harvard, Vancouver, ISO, and other styles

4

Gehmlich, Katja. "Strukturen der Kraftübertragung im quergestreiften Muskel : Protein-Protein-Wechselwirkungen und Regulationsmechanismen." Phd thesis, Universität Potsdam, 2004. http://opus.kobv.de/ubp/volltexte/2005/257/.

Full text

Abstract:

Im Mittelpunkt dieser Arbeit standen Signaltransduktionsprozesse in den Strukturen der Kraftübertragung quergestreifter Muskelzellen, d. h. in den Costameren (Zell-Matrix-Kontakten) und den Glanzstreifen (Zell-Zell-Kontakten der Kardiomyozyten).<br><br>Es ließ sich zeigen, dass sich die Morphologie der Zell-Matrix-Kontakte während der Differenzierung von Skelettmuskelzellen dramatisch ändert, was mit einer veränderten Proteinzusammensetzung einhergeht. Immunfluoreszenz-Analysen von Skelettmuskelzellen verschiedener Differenzierungsstadien implizieren, dass die Signalwege, welche die Dynamik de

APA, Harvard, Vancouver, ISO, and other styles

5

Gehmlich, Katja. "Strukturen der Kraftübertragung im quergestreiften Muskel Protein-Protein-Wechselwirkungen und Regulationsmechanismen /." [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=974875147.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Scheufler, Clemens. "Struktur-Funktions-Analyse von Hop Tpr-vermittelte Protein-Protein-Wechselwirkungen /." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=962034444.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Becker, Marion. "Bedeutung eines hydrophoben Seitenkettenstapels für Stabilität, Faltung und Struktur des P22 Tailspikeproteins." Phd thesis, Universität Potsdam, 2009. http://opus.kobv.de/ubp/volltexte/2010/4267/.

Full text

Abstract:

Das homotrimere Tailspikeadhäsin des Bakteriophagen P22 ist ein etabliertes Modellsystem, dessen Faltung, Assemblierung und Stabilität in vivo und in vitro umfassend charakterisiert ist. Das zentrale Strukturmotiv des Proteins ist eine parallele beta-Helix mit 13 Windungen, die von einer N‑terminalen Kapsidbindedomäne und einer C‑terminalen Trimerisierungsdomäne flankiert wird. Jede Windung beinhaltet drei kurze beta-Stränge, die durch turns und loops unterschiedlicher Länge verbunden sind. Durch den sich strukturell wiederholenden, spulenförmigen Aufbau formen beta-Stränge benachbarter Windun

APA, Harvard, Vancouver, ISO, and other styles

8

Valenta, Martin. "Predikce proteinových domén." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2013. http://www.nusl.cz/ntk/nusl-236163.

Full text

Abstract:

The work is focused on the area of the proteins and their domains. It also briefly describes gathering methods of the protein´s structure at the various levels of the hierarchy. This is followed by examining of existing tools for protein´s domains prediction and databases consisting of domain´s information. In the next part of the work selected representatives of prediction methods are introduced. These methods work with the information about the internal structure of the molecule or the amino acid sequence. The appropriate chapter outlines applied procedure of domains´ boundaries prediction.

APA, Harvard, Vancouver, ISO, and other styles

9

Plaga, Michal. "Bioinformatický nástroj pro predikci struktury proteinů." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2016. http://www.nusl.cz/ntk/nusl-255468.

Full text

Abstract:

The goal of this thesis is test and comparation of the offline tools for prediction of protein structure and creation of metaprediktor, which allows the user to select the appropriate tool, according to given parameters. Testing tool is based on a dataset of proteins, which is based on the SCOP database and it is trying to be as balanced as possible to include proteins from different families and thus could best evaluate individual tools. The results of this thesis are requirements of metaprediktor and also which data and settings can be allowed and processed and how it will be implemented.

APA, Harvard, Vancouver, ISO, and other styles

10

Villem, Lukáš. "Webový server pro predikci sekundární struktury proteinů." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2013. http://www.nusl.cz/ntk/nusl-236159.

Full text

Abstract:

This master’s thesis deals with protein secondary structure prediction. There is a theoretical introduction followed by study of available tools, proposal and implementation of web application, which combines functionality of several web tools used to predict secondary structure. User is asked to choose prediction methods and insert input sequence as plain text or upload a file. Results collected from selected tools serve to convert data into common format, show the result and create new type of prediction. Finally, the testing is applied and influences of tools are adjusted in order to

APA, Harvard, Vancouver, ISO, and other styles

11

Brigant, Vladimír. "Predikce sekundární struktury proteinů pomocí celulárních automatů." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2013. http://www.nusl.cz/ntk/nusl-236230.

Full text

Abstract:

This work describes a method of the secondary structure prediction of proteins based on cellular automaton (CA) model - CASSP. Optimal model and CA transition rule parameters are acquired by evolutionary algorithm. Prediction model uses only statistical characteristics of amino acids, so its prediction is fast. Achieved results was compared with results of other tools for this purpose. Prediction cooperation with a existing tool PSIPRED was also tested. It didn't succeed to beat this existing tool, but partial improvement was achieved in prediction of only alpha-helix secondary structure motif

APA, Harvard, Vancouver, ISO, and other styles

12

Votroubek, Lukáš. "Webový server pro predikci 3D struktury proteinu." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2013. http://www.nusl.cz/ntk/nusl-236225.

Full text

Abstract:

This work deals with proteins, especially with their structure and kinds of tertiary, or 3D, structure prediction. Tertiary structure prediction is very important for function prediction of this vitally important substance. Bioinformatics do this prediction much more effective and faster, because classical methods of structure prediction directly from molecule are very expensive and slow. On the other hand they are much more exact. Objective of this thesis is to describe tertiary structure prediction methods, describe used tools and possibility of automatic communication with them. Next objec

APA, Harvard, Vancouver, ISO, and other styles

13

Gentzel, Marc. "Proteine des männlichen Genitaltraktes Struktur und Funktion /." [S.l.] : [s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=960180419.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Quack, Thomas. "Analyse von Struktur-Funktionsbeziehungen bei der Ras-abhängigen Raf-Aktivierung." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964989735.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Schergaut, Marion Petra. "Struktur-, Funktionsanalyse und Ubiquitinierung des Ubiquitin-ähnlichen Modifizierers SUMO1." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=973065117.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Möhrlen, Frank. "Analyse der Struktur, Funktion und Evolution der Astacin-Protein-Familie." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=965165582.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Lin, Yi-Jan. "Solution structure of the 30 kDa homodimeric sud protein from Wolinella succinogenes." [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=968620817.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Mischke, Ralf. "Makrophagen migrationsinhibierender Faktor (MIF) : Struktur- und Aktivitätsstudien /." [S.l. : s.n.], 2000. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB8550795.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Weikl, Martina. "Der ATPase-Zyklus von Hsp90 und die Struktur-Funktions-Beziehung seines Partnerproteins p23." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961530391.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Gohlke, Holger. "Entwicklung einer wissensbasierten Bewertungsfunktion zur Struktur- und Affinitätsvorhersage von Protein-Ligand-Komplexen." [S.l.] : [s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=962391123.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Peti, Wolfgang. "Neue Methoden zur Bestimmung von NMR-Projektionsrestraints Bestimmung der Struktur und Dynamik von nativen und denaturierten Proteinen /." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963582704.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

De, Antoni Anna. "Structural and functional analysis of yeast proteins involved in ER-to-Golgi transport Sec24p family proteins and the GTPase activating protein Gyp5p /." Doctoral thesis, [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=962093610.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Wieske, Martin. "Beiträge zur Struktur und Funktion des kleinen Säuger-Streßproteins HSP25 unter besonderer Berücksichtigung der Wechselwirkung mit Actin." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 1998. http://dx.doi.org/10.18452/14452.

Full text

Abstract:

HSP25 ist ein Vertreter der ubiquitär verbreiteten kleinen Hitzeschockproteine, einer Familie innerhalb der großen Klasse der Streßproteine. Es ist an der Vermittlung von zellulärer Streßtoleranz beteiligt, besitzt Chaperoneigenschaften, hemmt die Actinpolymerisation in vitro und ist in der Lage, supramolekulare Komplexe auszubilden. Die hier vorgelegte Arbeit befaßte sich mit der Isolierung, der strukturellen und funktionellen Charakterisierung des Proteins und seinem Vorkommen in verschiedenen Geweben von Ratten mit pathologisch erhöhtem Blutdruck: · Es wurde eine Methode zur schonenden I

APA, Harvard, Vancouver, ISO, and other styles

24

Gregor, Jiří. "Strukturní studie inhibičních mechanismů kinas fosfatidylinositolu." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-379511.

Full text

Abstract:

+ssRNA viruses after entering the cell develop platforms for RNA replication called replication organelles. Due to the activity of phosphatidylinositol 4-kinases is in these areas a higher concentration of PI4P, which establishes suitable binding environment for the viral polymerase 3DPOL . One of these kinases is PI4KB, which is recruited to the membrane by the ACBD3 protein, which is itself recruited by giantin. Some kobuviruses and enteroviruses from the Picornaviridae family use their 3A protein to displace ACBD3 protein from the complex with giantin and transfer it from Golgi aparathus to

APA, Harvard, Vancouver, ISO, and other styles

25

Pšenáková, Katarína. "Strukturní studie vybraných komplexů signálních proteinů." Doctoral thesis, 2019. http://www.nusl.cz/ntk/nusl-394065.

Full text

Abstract:

The ability of proteins to bind other molecules in response to various stimuli in their microenvironment serves as a platform for extensive regulatory networks coordinating downstream cell actions. The correct function of these signaling pathways depends mostly on noncovalent interactions often affecting the structure of proteins and protein complexes. Understanding the molecular mechanism of a protein function in cell signaling therefore often depends on our knowledge of a three-dimensional structure. In this doctoral thesis, I present the work that led to the understanding of several protein

APA, Harvard, Vancouver, ISO, and other styles

26

Ptáčková, Barbora. "Strukturní charakterizace vybraných náhodných proteinových sekvencí s vysokým obsahem neuspořádanosti." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-379356.

Full text

Abstract:

An infinitesimal fraction of the practically infinite sequence space has achieved enormous functional diversity of proteins during evolution. Intrinsically disordered proteins (IDPs) which lack a fully defined three-dimensional structure are the most likely precursors to today's proteins because of their flexible conformation and functional diversity. But how have these proteins evolved into often rigid and highly specialized protein structures? This evolutionary trajectory has the greatest support in the theory of induced fold whereby the development of the structure was mediated by the inter

APA, Harvard, Vancouver, ISO, and other styles

27

Břicháčková, Kateřina. "Využití anotací primární struktury pro strukturní predikci protein-ligand aktivních míst." Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-438360.

Full text

Abstract:

The number of experimentally resolved protein structures in the Protein Data Bank has been growing fast in the last 20 years, which motivates the develop- ment of many computational tools for protein-ligand binding sites prediction. Binding sites prediction from protein 3D structure has many important applica- tions; it is an essential step in the complex process of rational drug design, it helps to infer the side-effects of drugs, it provides insight into proteins biological functions and it is helpful in many other fields, such as protein-ligand docking and molecular dynamics. As far as we k

APA, Harvard, Vancouver, ISO, and other styles

28

Polák, Marek. "Využití radikálového značení bílkovin pro strukturní biologii." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-434081.

Full text

Abstract:

(In English) The reaction of highly reactive oxygen radicals with protein solvent-accessible residues can be utilized to map protein landscape. Fast photochemical oxidation of proteins (FPOP) is an MS- based technique, which utilizes highly reactive radical species to oxidize proteins and map protein surface or its interactions with their interaction partners. In this work, FPOP was employed to study protein-DNA interactions. First, a full-length of FOXO4-DBD was successfully expressed and purified. The ability of the protein to bind its DNA-response element was verified by electrophoretic and

APA, Harvard, Vancouver, ISO, and other styles

29

Hexnerová, Rozálie. "Strukturní NMR studie proteinových komplexů." Doctoral thesis, 2019. http://www.nusl.cz/ntk/nusl-409282.

Full text

Abstract:

Protein-protein interactions are involved in various biological processes and detailed characterization of their structural basis by the means of structural biology is often instrumental for rigorous understanding of underlying molecular mechanisms. This information is important not only for fundamental biology but also plays an important role in search for sites amenable for therapeutic intervention. Nuclear magnetic resonance spectroscopy is alongside X-ray crystallography and single-particle cryo-electron microscopy one of the key high-resolution techniques in structural biology. Although i

APA, Harvard, Vancouver, ISO, and other styles

30

Koupilová, Nicola. "Strukturní charakterizace lidské proteinkinasy CaMKK2 a jejích interakcí s vazebnými partnery." Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-448762.

Full text

Abstract:

5 Abstract Ca2+/calmodulin-dependent protein kinase kinase 2 (CaMKK2) belongs to the serine/ threonine protein kinase family, which is involved in the calcium signaling pathway. The increase of intracellular calcium concentration induces the activation of calmodulin (CaM), which then activates its binding partners including CaMKII, CaMKIII, CaMKK1 and CaMKK2. CaMKK2 activates CaMKI, CaMKIV and AMP-dependent kinase, AMPK, by phosphorylation. CaMKK2 is naturally present in cells in an autoinhibited state, which is caused by the steric hindrance of the active site by the autoinhibitory domain. Wh

APA, Harvard, Vancouver, ISO, and other styles

31

Horníková, Lenka. "Hlavní strukturní protein myšího polyomaviru: interakce s buněčnými strukturami." Doctoral thesis, 2012. http://www.nusl.cz/ntk/nusl-309830.

Full text

Abstract:

Mouse polyomavirus (MPyV) is small non-enveloped DNA virus. Although this virus has been studied for almost 60 years, it still remains unclear, how can virus transport its genetic information to the cell nucleus. Also, the mechanism of virion morphogenesis is not well understood. First part of this work is focused on endocytic pathway which is used by MPyV for trafficking toward the cell nucleus. Using dominant negative mutant of caveolin-1 we showed that caveolin-1dependent endocytic pathway, described for SV40, is not used by MPyV for productive infection. MPyV is transported to early endoso

APA, Harvard, Vancouver, ISO, and other styles

32

Dubánková, Anna. "Strukturní charakterizace replikace RNA lidského Aichi viru." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-345050.

Full text

Abstract:

Viral RNA dependent RNA polymerases (RdRps) are enzymes which enable RNA viruses to replicate their genome and to prepare mRNA for translation of viral proteins. Due to its relative evolutionary conservation RdRps are good targets for drug design. In this work we present a structure of the RdRp (3Dpol ) of Aichi virus, which has not been solved yet. Aichi virus is a human pathogen that causes gastroenteritis. Aichi virus is also used as a model organism for studying cognate viruses which virulence is more dangerous, for example: Rhinovirus, Hepatitis A virus, SARS virus, hepatitis C virus, yel

APA, Harvard, Vancouver, ISO, and other styles

33

Pavlásková, Kateřina. "Hmotnostní spektrometrie v proteomice: strukturní biologie a klinické aplikace." Doctoral thesis, 2011. http://www.nusl.cz/ntk/nusl-311574.

Full text

Abstract:

Mass spectrometry (MS) is a rapid, specific and very sensitive analytical method with a broad spectrum of proteomic applications such as protein identification and sequencing, 3D protein structure characterization or study of protein-protein interaction. The introduction of two ionization techniques in late 1980's that are able to ionize the large biomolecules such as proteins, oligosaccharides or nucleic acids with no or low fragmentation has started the rapidly expanding field of MS-based proteomics. The presented thesis was aimed at the application of mass spectrometric approaches to answer

APA, Harvard, Vancouver, ISO, and other styles

34

Hausner, Jiří. "Rekombinantní expresse chloridového kanálu z E. coli a jeho strukturní charakterizace." Master's thesis, 2014. http://www.nusl.cz/ntk/nusl-340381.

Full text

Abstract:

Chloride channel family has been shown to play a significant role in physiological homeostasis processes. The function mechanism of these proteins has not yet been clearly understood. Their deficiency or mutation causes serious human illnesses. Our understanding of the chloride channels' transporting mechanisms can lead to better treatment of these illnesses. As mammalian chloride channels are difficult to prepare in laboratory, the experiments are usually done on homologous chloride channels from prokaryotic organisms. The structures of prokaryotic chloride channels have been solved and moreo

APA, Harvard, Vancouver, ISO, and other styles

35

Těšina, Petr. "Strukturní studie interakcí proteinu LEDGF/p75." Doctoral thesis, 2016. http://www.nusl.cz/ntk/nusl-347466.

Full text

Abstract:

3 ABSTRACT LEDGF/p75 protein is a human transcriptional co-activator and epigenetic reader associated with transcriptionally active chromatin. It is crucial for HIV integration and MLL1 fusion-driven leukemia development. Interactions of LEDGF/p75 with HIV integrase (HIV IN) and MLL1-menin complex are considered an attractive therapeutic target for drug development. LEDGF/p75 interacts with both HIV IN and MLL1-menin complex through its integrase binding domain (IBD). While the pathophysiological interactions of LEDGF/p75 IBD were intensively studied, little was known about the physiological o

APA, Harvard, Vancouver, ISO, and other styles

36

Vaňková, Pavla. "Strukturní charakterizace intracelulární formy myšího Nkr-p1a proteinu." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-343063.

Full text

Abstract:

NK cells are a component of innate immunity system, which is derived from lymphoid progenitor. By a sophisticated receptor repertoire, which is expressed on their surface, they provide a surveillance against pathogenic, virus infected or tumour cells. Simultaneously they produce cytokines, thereby are involved in adaptive immune response. This work is focused on the study of structure of mice soluble mNkr-p1a isoform. Recently this short isoform was identified at the transcriptional level by a member of our laboratory and it is designated as isoform 2. The aim was to produce mNkr-p1a iso2 prot

APA, Harvard, Vancouver, ISO, and other styles

37

Šmídová, Aneta. "Příprava a charakterizace neutrální trehalasy pro strukturní studie." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-342003.

Full text

Abstract:

This study is part of a project which aim is solving the structure of the catalytic domain of neutral trehalase Nth1 from Saccharomyces cerevisiae. The main goal of this thesis is the preparation of new constructs of yeast Nth1 and optimization of their purification protocols, the selection of the ideal buffer for crystallization trials using the method of differential scanning fluorimetry (DSF) and at last the protein crystallization. Another part of the thesis is the measurement of the enzymatic activity of pNth1 WT in the presence of Bmh1 protein, verification of trehalose binding to the se

APA, Harvard, Vancouver, ISO, and other styles

38

Dostalík, Pavel. "Role acetylace proteinů v životním cyklu Polyomavirů." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-416106.

Full text

Abstract:

Capsid of mouse polyomavirus (MPyV) is composed from three structural proteins: major structural protein VP1 and minor structural proteins VP2 and VP3. Posttranslational modifications may affect functions of proteins. This work deals with acetylation of MPyV structural proteins and its impact on the viral replication cycle. First part of the thesis is focused on acetylation of VP1. We showed that the VP1 protein is acetylated in viral particles and that interaction of VP1 with minor proteins supports VP1 acetylation. Further, we showed that cytoplasmatic deacetylase, histone deacetylase 6 (HDA

APA, Harvard, Vancouver, ISO, and other styles

39

Dvorská, Anna. "Strukturní biologie komplexu potkaních NK buněčných receptorů NKR-P1B a Clrb." Master's thesis, 2014. http://www.nusl.cz/ntk/nusl-338187.

Full text

Abstract:

The Natural Killer (NK) cells have an important role in the nonspecific immunity of the or- ganism. They have the ability to identify and to kill tumor cells and cells infected by a virus without preceding sensitization by antigen. Their function is directed by the amount of sti- mulation and inhibition receptors interacting with ligands on the tumor or infected cell. This thesis focuses on the preparation and the study of the complex of rat NK cellular inhi- bition receptor NKR-P1B ("natural killer cell receptor - protein 1B") and its ligand Clrb ("C-type lectin-related ligand b"). The Clrb i

APA, Harvard, Vancouver, ISO, and other styles

40

Kielarová, Anežka. "3D struktury fosforylace." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-396719.

Full text

Abstract:

Protein phosphorylation is a common post-translational protein modification used in almost all cellular processes. When a phosphate group is added to an amino acid side chain, it may alter the protein conformation and protein-protein interactions due to its size and its negative charge. It may also change the protein function, activity and even localization within the cell. Experimental detection of phosphorylation is still extremely labor demanding and very expensive, even when deploying protein mass spectrometry. For this very reason many bioinformatics scientific groups focus on the predict

APA, Harvard, Vancouver, ISO, and other styles

41

Duchoslav, Vojtěch. "Biofyzikální charakterizace proteinového komplexu CAR/RXR s jeho vazebnými partnery." Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-448876.

Full text

Abstract:

The constitutive androstane receptor (CAR) plays an important role as a xenosensor in the organism and is therefore widely expressed in the kidney, liver, gallbladder and in the small intestine epithelium, where the biotransformation of xenobiotic occurs. CAR is also an important factor in the elimination of bile acids and bilirubin. CAR has also been shown to have an important role in regulation of glucose and lipid metabolism. Impairment of lipid and glucose metabolism is a common cause of cardiovascular and metabolic diseases such as atherosclerosis, type 2 diabetes, obesity and insulin res

APA, Harvard, Vancouver, ISO, and other styles

42

Skála, Kristián. "Studium extracelulární části myšího receptoru Nkr-p1b přirozených zabíječských buněk pomocí NMR." Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-354623.

Full text

Abstract:

Protein Nkr-p1b is a surface receptor of cytotoxic NK cells, that mediates inhibitory signal toward the body's own cells. In this study, the ligand binding domain of the mouse protein receptor Nkr-p1b (mNkr-p1b LBD) was prepared by recombinant expression in E. coli cells. Isolated protein was subsequently used for NMR structural analysis. Prediction of protein secondary structures ratio was carried out using three different methods (CD, PSIPRED and TALOS). Results correlate well with the structure of CTLD domain, that plays a key role in ligand binding and thus to function of Nkr-p1b receptor.

APA, Harvard, Vancouver, ISO, and other styles

43

Bílková, Eva. "Studium vlastností minoritních strukturních proteinů myšího polyomaviru." Master's thesis, 2014. http://www.nusl.cz/ntk/nusl-332400.

Full text

Abstract:

Murine polyomavirus (MPyV) is a member of the Polyomaviridae family. Its capsid is composed of the major capsid protein, VP1, and the minor proteins, VP2 and VP3. The minor capsid proteins probably assure delivery of the viral genome through the endoplasmic reticulum membrane to the nucleus during early phase of infection. However, precise mechanism is not known. Expression plasmids encoding mutated VP2 or VP3 fused with EGFP have been constructed to study the interaction of VP2 and VP3 with membranes. The mutated proteins have deletions in the predicted hydrophobic domains. In this thesis, ce

APA, Harvard, Vancouver, ISO, and other styles

44

Jakubec, Dávid. "Interakce Proteinů s Nukleovými Kyselinami: od Struktury k Specificitě." Doctoral thesis, 2020. http://www.nusl.cz/ntk/nusl-436911.

Full text

Abstract:

Sequence-specific interactions between proteins and nucleic acids play an essential role in the cell biology. While several molecular mechanisms contributing to the binding speci- ficity have been identified empirically, no general protein-DNA recognition code has been described to date. In this thesis, I explore selected characteristics of protein-DNA inter- actions using computational methods. First, the pairwise interactions between the basic biomolecular building blocks-amino acids and nucleotides-are investigated. It is shown that several statistically enriched, biologically relevant inte

APA, Harvard, Vancouver, ISO, and other styles

45

Sauerová, Pavla. "Studium vlastností genových produktů Polyomaviru karcinomu Merkelových buněk : Příprava protilátek a konstrukce expresních vektorů." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-322774.

Full text

Abstract:

Merkel cell polyomavirus (MCPyV) is a recently discovered human virus, having it's genome often integrated in a genome of Merkel carcinoma cells. Although this type of carcinoma is not so usual, it is very aggressive and it's incidence has been rising in last few years. It is not surprising that this virus is nowadays in the centre of scientific interest, as well as other pathogens and mechanisms affecting human life. Because the virus was discovered not so long ago, its research has been at the whole beginning. This diploma thesisaims to contribute to the study of this virus from the molecula

APA, Harvard, Vancouver, ISO, and other styles

46

Riedlová, Kamila. "Zjišťování struktury pórotvorných kolicinů." Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-368460.

Full text

Abstract:

6 Abstract This master's thesis provides study of individual helixes from C-terminal pore-forming domain (CTD) of colicin U and their behavior in lipid bilayer on atomic level. For this purpose the all-atom molecular simulation method was used. Later the study was extended an applied on CTD of published structures of other pore-forming colicins. On the base of study extension the ability of disruption of lipid bilayer integrity by helixes H1 and H10 was successfully observed. Helix H1 was synthesized and its activity was experimentally proved on black lipid membranes. The other helixes are oft

APA, Harvard, Vancouver, ISO, and other styles

47

Kylarová, Salome. "Studium molekulárních mechanismů regulace signálních proteinů." Doctoral thesis, 2018. http://www.nusl.cz/ntk/nusl-384184.

Full text

Abstract:

EN The aim of this study was to investigate the regulatory mechanisms of two important signaling proteinkinases and promising therapeutic targets, ASK1 and CaMKK2. ASK1 kinase is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family that activates c-JNK kinase and p38 MAP kinase pathways in response to various stress stimuli, including oxidative stress. The function of ASK1 is associated with the activation of apoptosis and thus plays a key role in the pathogenesis of multiple diseases including cancer, neurodegeneration or cardiovascular diseases. The natural inhibitor

APA, Harvard, Vancouver, ISO, and other styles

48

Fojtík, Lukáš. "Využití radikálového značení proteinů pomocí Togniho činidel ve strukturní biologii." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-434032.

Full text

Abstract:

Structural proteomic methods such as an ion mobility mass spectrometry, chemical cross- linking or covalent labeling have been established as powerful tools for structural studies of biomolecules in general. These methods have significantly contributed to the expansion of our knowledge about biomolecular functions, their dynamics and molecular interactions and therefore led to the understanding of important biological processes occurring in a cell. We decided to spread these methods and we developed a new radical labeling technique relaying on Fluor-alkyl radicals that does not require a laser

APA, Harvard, Vancouver, ISO, and other styles

49

Vinšová, Barbora. "Minoritní strukturní proteiny polyomavirů: Vlastnosti a interakce s buněčnými strukturami." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-343879.

Full text

Abstract:

Even though polyomaviruses have been intensively studied for more than 60 years, the role of minor structural proteins VP2 and VP3 in some important steps of viral life cycle has still not been fully elucidated, explicitly their role in viral genome delivery to the cell nucleus and their involvement in late phases of viral life cycle. This diploma thesis focuses on the study of minor proteins of Mouse polyomavirus (MPyV) and Human polyomavirus BK (BKV). Four rabbit polyclonal antibodies against minor proteins of polyomaviruses MPyV or BKV have been prepared within this diploma thesis. Two of t

APA, Harvard, Vancouver, ISO, and other styles

50

Radilová, Kateřina. "Analýza strukturních detailů NMDA receptoru." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-388403.

Full text

Abstract:

NMDA receptor is necessary for excitatory transmission in the central nervous system. Altered funtion of the NMDA receptors is associated with many neurodegenerative and neuropsychiatric diseases. All available crystal structures of the NMDAR meant great shift towards our understanding of details of the receptor and its function. Unfortunately, these up- to-date available structures present only certain functional states of receptors and also a few structural data are still missing. For complete comprehension of the process of activation and deactivation of NMDA receptors, we need to supplemen

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!