Academic literature on the topic 'Sub-conjunctiva'

The incidence of squamous cell carcinoma of the conjunctiva is particularly high in sub-Saharan Africa with temporal trends similar to those of Kaposi sarcoma (KS). Human herpesvirus type 8 (HHV8), has not yet been investigated in conjunctiva tumors. In this study biopsies and PBMCs of conjunctiva neoplasia patients along with nonneoplastic conjunctiva tissues have been analyzed for HHV8 sequences by PCR targeting ORF26. All amplimers were subjected to nucleotide sequencing followed by phylogenetic analysis. HHV8 DNA has been identified in 12 out of 48 (25%) HIV-positive, and in 2 out of 24 (8.3%) HIV-negative conjunctiva neoplastic tissues and in 4 out of 33 (12.1%) PBMC samples from conjunctiva neoplasia diseased patients as well as in 4 out of 60 (6.7%) nontumor conjunctiva tissues. The viral load ranged from 1 to 400 copies/105cells. Phylogenetic analysis showed that the majority of HHV8 ORF26 amplimers clustered with subtypes R (n=11) and B2 (n=6). This variant distribution is in agreement with that of HHV8 variants previously identified in Ugandan KS cases. The presence of HHV8 in conjunctiva tumors from HIV-positive patients warrants further studies to test whether HHV8 products released by infected cells may have paracrine effects on the growth of conjunctiva lesions.

Ocular surface inflammatory disorder (OSID) is a spectrum of disorders that have features of several etiologies whilst displaying similar phenotypic signs of ocular inflammation. They are complicated disorders with underlying mechanisms related to several autoimmune disorders, such as rheumatoid arthritis (RA), Sjögren’s syndrome, and systemic lupus erythematosus (SLE). Current literature shows the involvement of both innate and adaptive arms of the immune system in ocular surface inflammation. The ocular surface contains distinct components of the immune system in the conjunctiva and the cornea. The normal conjunctiva epithelium and sub-epithelial stroma contains resident immune cells, such as T cells, B cells (adaptive), dendritic cells, and macrophages (innate). The relative sterile environment of the cornea is achieved by the tolerogenic properties of dendritic cells in the conjunctiva, the presence of regulatory lymphocytes, and the existence of soluble immunosuppressive factors, such as the transforming growth factor (TGF)-β and macrophage migration inhibitory factors. With the presence of both innate and adaptive immune system components, it is intriguing to investigate the most important leukocyte population in the ocular surface, which is involved in immune surveillance. Our meta-analysis investigates into this with a focus on both infectious (contact lens wear, corneal graft rejection, Cytomegalovirus, keratitis, scleritis, ocular surgery) and non-infectious (dry eye disease, glaucoma, graft-vs-host disease, Sjögren’s syndrome) situations. We have found the predominance of dendritic cells in ocular surface diseases, along with the Th-related cytokines. Our goal is to improve the knowledge of immune cells in OSID and to open new dimensions in the field. The purpose of this study is not to limit ourselves in the ocular system, but to investigate the importance of dendritic cells in the disorders of other mucosal organs (e.g., lungs, gut, uterus). Holistically, we want to investigate if this is a common trend in the initiation of any disease related to the mucosal organs and find a unified therapeutic approach. In addition, we want to show the power of computational approaches to foster a collaboration between computational and biological science.

Cysticercosis is a parasitic infestation caused by cysticercus cellulose. Ocular and adnexal cysticercosis represents 13% to 46% of systemic disease. There are different modes of presentation of ocular cysticercosis. Case: Here we report a case with the left upper eyelid ptosis. On ocular examination, there was presence of a cystic yellowish mass of size of the eye ball in the upper palpebral sub-conjunctiva along with dystropia and limitation of extraocular movements in dextroelevation. Complete surgical excision of the cyst was achieved through splitting of the left upper eyelid. There was presence of sub-conjunctival cyst along with involvement of levator palpebral superioris (LPS) and superior rectus (SR) muscle. Histopathological examination of the cyst showed features suggestive of cysticercosis. The patient was symptomatically better postoperatively. He was commenced on oral prednisolone 1mg/kg and oral albendazole 400 mg for 28 days. Conclusion: Cysticercosis should be considered in cases of inflammatory swelling of subconjunctival space especially in people with poor personal and community hygiene. DOI: http://dx.doi.org/10.3126/hren.v11i3.9659 Health Renaissance 2013;11(3):293-296

<b><i>Background:</i></b> Separately, cryotherapy and brachytherapy have shown promising results when adjuvating the excision of squamous cell carcinoma of the conjunctiva (SCCC). The aim of this paper is to assess the combined effect in terms of complications and recurrence rate. <b><i>Summary:</i></b> We describe 2 patients suffering from SCCC, and we review the current literature on adjuvant cryotherapy and brachytherapy. Both patients, one of whom suffered from recurrent SCCC, underwent surgical excision followed by combined cryotherapy and brachytherapy. Cryotherapy was performed using a retinal cryoprobe, and 2 rounds each of 5 s with N<sub>2</sub>O as a cryogen were applied. Brachytherapy was performed using a ruthenium-106 plaque, delivering a dosage of 100 Gy at 2-mm depth. <b><i>Key Messages:</i></b> By reviewing the current literature and describing 2 case reports, this paper illustrates the use of combined cryotherapy and brachytherapy after surgical excision of SCCC. The current literature presents promising results of each treatment, and the 2 cases showed promising results by combining the 2 adjuvant therapies showing no signs of recurrence or complications during a follow-up period of 26 and 38 months.

Dydetsyldymetylammonium chloride (DDAC) is used as a raw material in the chemical industry for the production of some insecticidal, fungicidal and aldehydic preparations and disinfectants in which it is an active ingredient. Aim of the Research. To establish a scientific substantiation for DDAC indicative safe exposure level (ISEL) in the workplace air by analyzing and summarizing the bibliographic data about the degree of DDAC danger. Methods. To establish a scientific substantiation for DDAC occupational exposure standard the following properties were analyzed: its physical and chemical characteristics; LD50 (lethal dose) for oral and dermal exposure; LC50 (lethal concentration) for inhalation exposure; irritant and sensitizing properties; NOAELs (no-observed adverse effect level) for DDAC were determined in sub-acute and subchronic experiments under different conditions of exposure as well as its long-term effects. The calculation of DDAC ISEL in the workplace air was performed using equations which make allowance for the toxicity properties of the preparation when it is administered to laboratory animals through different routes. Results. DDAC is an imported product used as an active ingredient in the production of certain pesticides and disinfectants. DDAC falls into the category of non-volatile substances: its vapor pressure is <1x10-2 Pa at 20° C, at 50оC – 2,3x10-4 Pa (US ISC) When introduced into the gastrointestinal tract, the substance is classified as moderately dangerous – Category 3 (in accordance with Globally Harmonized System of Classification and Labeling of Chemicals / GHS / United Nations, New York and Geneva, 2017) and has a mild skin-resorptive effect (Category 4, GHS). Its adverse local impact on the skin has been identified. The substance LC50 after inhalation exposure is 70 mg/m3 (Category 2, GHS). DDAC produces marked irritant action on the skin and conjunctiva of rabbits (Category1B, GHS). Erythema, crust and swelling were recorded on skin. Burns, corrosion, acute keratoconjunctivitis were observed when the substance got onto conjunctiva. No sensitizing effect of the preparation was found (not classified, GHS). The cumulative properties of the substance are incomplete. NOAELs were determined in sub-acute and sub-chronic experiments after oral, dermal and inhalation exposure. The most affected organs and systems are skin, eyes, gastrointestinal tract, liver. Mutagenic, carcinogenic, embryotoxic, teratogenic and toxic effects on the reproductive function are not limiting harmful criteria. Calculation of the value of DDAC ISEL in the workplace air was conducted by the equations of regressive dependence, which take into consideration the parameters of acute toxicity. Making allowance for the degree of its hazard and the risk of developing the pathology of general genesis under the effect of the substance, the recommended ISEL value in the workplace air is 0.2 mg/m3, aggregate state “a” with the mark “protection of eyes and skin is necessary”. Key Words: dydetsyldymetylammonium chloride, DDAC, ISEL in the workplace air, insecticides, disinfectants.

Objective:To describe the combined technique of midface lift and partial release of orbital fat for facial rejuvenation and evaluate the safety and aesthetic results.Methods:This was a single-center study that included patients who underwent facial rejuvenation from January 2010 to December 2016. The orbital fat was partially removed, and the cheek fat pads were repositioned to exfoliate the myocutaneous flaps of the lower eyelids and midface through a sub-ciliary incision. The cheek soft tissue pads and orbital fat were restored and sutured to the periorbital membranes and orbital margins. Postoperatively, the patients were examined at 6 and 18 months for changes in the shapes of cheeks, eyelid joints, sunken eyepit, and nasolabial sulcus along with procedural complications.Results:A total of 179 (177 women; 2 men) eligible patients with a mean age of 47.17 years underwent the procedure. The shapes of cheeks and eyelid joints of all patients improved, along with shallowing of the sunken eyepit and nasolabial sulcus at 6- and 18-month follow-up. The surgical procedure involved minor trauma without facial scarring, lower eyelid flinch, and dislocation. None of the patients reported complications of nerve injuries, long-term chemosis conjunctiva, periocular swelling, or dislocation of the lower eyelids. Only 2 patients who underwent subsequent pouch surgeries had slight ectropion, which was restored within 4 months of surgery.Conclusion:To conclude, the combined partial orbital fat repositioning and midface lift via sub-ciliary approach is a successful treatment option for facial rejuvenation with no major complications.

Introduction Orbital masses in adults are often caused by systemic diseases or are associated with systemic manifestations. Juvenile xanthogranuloma as a cause is rare and unreported in Africa. We present clinical features, management, and outcomes of bilateral orbital adult onset juvenile xanthogranuloma. Case Presentation A 27 year old Nigerian woman presented with bilateral upper-lid lumps having lasted 5 months. These increased in size for about 1 month and stopped. Lid swelling was preceded by itchy eyes, redness of conjunctiva, and occasional mild pain. There were no visual or systemic symptoms. The lumps were firm, slightly mobile, not tender, and not attached to skin but rather to deeper structures. There was restriction on up-gaze but no proptosis or diplopia. Hematological, biochemical, and X-ray investigations were normal. Prednisolone tablets 10 mg daily for two weeks were not useful. Tissue biopsy was invaluable in diagnosis of this rare condition and disclosed juvenile xanthogranuloma. Partial surgical excision was done under lidocaine infiltration. No recurrence has occurred in 40 months of follow-up. No systemic disease has manifested. Conclusion Juvenile xanthogranuloma can present as bilateral superior orbital tumor in adults; functional and cosmetic aims were achieved by sub-total excision.

Background: Kissing nevus is a congenital nevus in adjacent parts of the eyelids. Malignant transformation of kissing or divided nevi of the eyelids is rarely described. Objective: To report a very rare case of malignant transformation of kissing nevus with ocular and extraocular spread. Case: A 57- year- old man with 6/6 visual acuity in both eyes presented with a kissing nevus present since birth in right upper and lower eyelids which had a slow growth phase. The upper lid in the area of the nevus was thickened with a 20x12x15 mm black pigmented crusted hemorrhagic nodular lesions. The lower lid had a 6 mm black pigmented ulcerated lesion over the pre-existing nevus in the lateral third of the lid with full thickness infiltration. Another 5x4 mm pigmented lesion over the lower medial lid margin with a thickness of about 3 mm extended to the conjunctival side of the lower lid. Right sided pre-auricular and submaxillary nodes were palpable. A biopsy of tissue samples from the eyelid and pre-auricular nodes were consistent with malignant melanoma.Conclusion: Malignant transformation of kissing nevus is rare. It can spread to the conjunctiva, pre-auricular and sub-mandibular lymphnodes.DOI: http://dx.doi.org/10.3126/nepjoph.v4i2.6555 Nepal J Ophthalmol 2012; 4 (2): 329-332

Background: Pterygium, derived from the Greek work ‘pteryx’, meaning wing is a growth of fibrovascular tissue on the cornea and conjunctiva in the palpebral fissure. The present study was conducted to find out the prevalence and Socio-demographic determinants of pterygium in Sub Himalayan region of Himachal Pradesh, India.Methods: A cross-sectional study was conducted between the months of May-June 2018, in the outpatient department of Ophthalmology of Pt. Jawahar Lal Nehru Govt. Medical College and Hospital, Chamba, H.P, India. Among all the patients visiting the OPD in the given duration, a total of 200 patients having Pterygium were selected for the study. Detailed history of the all the patients were taken and required examination was done. Data collected was analysed with Microsoft excel software.Results: Out of the 200 patients, 59% were males and 41% were females. 70% of the total study population were seen in the age group 41-60years. In present study a total of 130 patients (65%) were outdoor workers. Left eye (40.5%) was the eye most frequently involved eye in present study, right eye (36.5%) also both the eyes were involved in 23% of study population. Nasal pterygium was seen in 93%, temporal pterygia 5.5% and double pterygium (both nasal and temporal presentation in same eye) in 1.5% patients. Grade I pterygium was seen in 43.5% patients, grade II in 40% and grade III pterygia were found in 16.5% of the study population.Conclusions: People living in high altitude regions and who work outdoors, are subjected to increased U.V. exposure. So, they must take appropriate precautions like wearing protective glasses and caps etc.

My PhD research involves multi-disciplinary areas of study such as measuring perfusion of blood vessels in hamster dorsal skin using laser speckle imaging technique. In this study the changes were measured in blood flow velocity and diameters of micro vasculatures after the influence of glycerol application. The second study identifies the changes in morphology and optical properties of eye tissue after applying hyper-osmotic agent such as 100% anhydrous glycerol. Further investigation on the reversal process was performed without any application of 0.9% saline. The third study identified the variation in fluorescence in hamster dorsal skin tissue and enucleated porcine eyes with temperature. This study investigated the variation in fluorescence intensity with temperatures starting at 14°C and compared in vivo and in vitro results for consistency. The fourth study investigated an implantable drug delivery package that was fabricated using PMMA and implanted between the sub-conjunctival and super-scleral space and release the content of the device by either mechanical pressure or light-activated ophthalmic Nd:YAG laser after optically clearing the eye tissue by topical application of a hyper-osmotic agent, 100% anhydrous glycerol. A hyper-osmotic agent creates a transport region in the conjunctiva and sclera to get visual access of the compartments in the drug delivery package. This new technology would provide the option to the patient of one time implantation of the carrier system containing the drug. Each time the patient requires medication a ND-YAG or other laser beam will propagate through the cleared eye tissue to release the drug in measurable doses at the discretion of the doctor from the package directly in to the vitreous humor. In this study we have measured half-life of the dye in the vitreous humor or posterior chamber and biocompatibility. The last study had drawn distinction between the fluorescence signals based on the location (anterior or posterior chamber) of the 10% Na fluorescence dye in the in vivo rabbit and ex vivo pig eyes.<br>text