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Dissertations / Theses on the topic 'Substrate rigidity'

Author: Grafiati

Published: 25 May 2024

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1

Wang, Guan. "Roles of substrate rigidity and composition in membrane trafficking." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCC195.

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Du cerveau à l’os, la rigidité et la composition de la matrice extracellulaire varient énormément et jouent un rôle dans les réponses cellulaires. La rigidité influe également sur la tension de la membrane plasmique, elle-même régulée par le trafic membranaire. Comment la rigidité et la composition du substrat peuvent réguler l'exocytose, qui à son tour régule la tension de la membrane, reste largement inconnu. Ici, j'ai utilisé l’imagerie pHluorin d’évènements uniques d’exocytose de cellules cultivées sur des substrats de rigidité et de composition contrôlée pour explorer la régulation de VAM

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2

Manifacier, Ian. "Understanding adherent cell mechanics and the influence of substrate rigidity." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4106/document.

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L’ingénierie tissulaire est une stratégie médicale qui repose sur la régénération de tissu par les cellules avec ou sans matériaux. Pour maîtriser cette synthèse, il faut comprendre la cellule comme une part intégrante du tissu. Hormis ses interactions biochimiques avec son support, la cellule interagit également mécaniquement avec son environnement. Elle s’accroche à ce dernier et évalue sa dureté pour adapter sa réponse biologique. Dans cette étude, j’ai développé des modèles numériques pour analyser l’influence de la rigidité du substrat sur le comportement mécanique de la cellule, sur sa s

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3

Frey, Margo Tilley. "Development of a Substrate with Photo-Modulatable Rigidity for Probing Spatial and Temporal Responses of Cells to Mechanical Signals: A Dissertation." Digital WPI, 2008. https://digitalcommons.wpi.edu/etd-dissertations/337.

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"Topographical and mechanical properties of adhesive substrates provide important biological cues that affect cell spreading, migration, growth, and differentiation. The phenomenon has led to the increased use of topographically patterned and flexible substrates in studying cultured cells. However, these studies may be complicated by various limitations. For example, the effects of ligand distribution and porosity are affected by topographical features of 3D biological constructs. Similarly, many studies of mechanical cues are compounded with cellular deformation from external forces, or li

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4

Dutour, Provenzano Gaëlle. "Role of intermediate filaments in mechanotransduction." Electronic Thesis or Diss., Sorbonne université, 2021. http://www.theses.fr/2021SORUS364.

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Les cellules s'adaptent en permanence à leur microenvironnement. En particulier, elles modifient leur morphologie, leur croissance, leur division et leur motilité en fonction des propriétés biochimiques et physiques de la matrice extracellulaire (MEC). Elles sont équipées de structures adhésives appelées plaques d’adhérences, permettant aux cellules d'interagir avec les protéines de la MEC via les protéines transmembranaires appelées intégrines et de détecter la nature et la rigidité de la MEC. Le signal est transduit par les protéines des plaques d’adhérences et résulte par exemple en une mod

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5

Ehlinger, Claire. "Influence de la rigidité du substrat sur la migration des cellules souches de la pulpe dentaire." Thesis, Strasbourg, 2020. http://www.theses.fr/2020STRAE001.

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La migration des cellules souches de la pulpe dentaire (DPSCs) est un aspect fondamental de l’ingénierie tissulaire dentaire. L’objectif de cette thèse est de déterminer l’influence de la rigidité du substrat sur la migration des DPSCs. Dans une première partie, nous avons montré que les DPSCs sont capables de survivre et de proliférer sur des substrats de polydiméthylsiloxane (PDMS) avec un module de Young de 1,5 kPa à 2,5 MPa sans se différencier. Nous avons observé que la vitesse moyenne des DPSCs est augmentée sur les substrats de faible rigidité. De plus, la Yes-associated protein (YAP) c

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6

Wang, Bin. "Réalisation et étude de substrates de rigidité modulable et de dispositifs intégrables pour l'ingénierie cellulaire et tissulaire." Thesis, Paris Sciences et Lettres (ComUE), 2017. http://www.theses.fr/2017PSLEE043/document.

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L’objectif de ce travail de thèse est de réaliser des substrats et des dispositifs de culture cellulaire pour des applications à grande échelle. En utilisant à la fois des techniques de lithographie conventionnelles et non conventionnelles, nous avons d'abord fabriqué des matrices denses de piliers élastomère avec un gradient de hauteur pour les études de migration cellulaire et nous avons observé un allongement cellulaire remarquable et une migration cellulaire dirigée, tout dépendant du gradient de rigidité. Les micropiliers élastomères pourraient également être organisés en gradient de haut

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7

Flick, Florence. "La plasticité de la chromatine oriente le destin des cellules saines et des cellules cancéreuses sur des matrices de faibles rigidités." Thesis, Strasbourg, 2016. http://www.theses.fr/2016STRAE020/document.

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L’objectif de cette thèse est d'étudier l'influence d'hydrogels de faibles rigidité sur l’organisation de la chromatine de cellules épithéliales PtK2 et cancéreuses SW480. Sur des hydrogels mous, la chromatine de PtK2 se structure en hétérochromatine. Les hydrogels très mous conduisent à la nécrose. Sur ces substrats, l'euchromatine, maintenue par inhibition de HDAC, guide la cellule en quiescence. Ces cellules se divisent après transfert sur surfaces rigides. Un processus de dissémination métastatique est développé en cultivant des cellules cancéreuses sur des hydrogels très mous (E20) et des

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8

Hovhannisyan, Yeranuhi. "Modélisation cardiaque des myopathies myofibrillaires à l'aide de cellules souches pluripotentes induites pour explorer la pathogenèse cardiaque Polyacrylamide Hydrogels with Rigidity-Independent Surface Chemistry Show Limited Long-Term Maintenance of Pluripotency of Human Induced Pluripotent Stem Cells on Soft Substrates Modéliser la myopathie myofibrillaire pour élucider la pathogenèse cardiaque Synemin-related skeletal and cardiac myopathies: an overview of pathogenic variants Desmin prevents muscle wasting, exaggerated weakness and fragility, and fatigue in dystrophic mdx mouse Effects of the selective inhibition of proteasome caspase-like activity by CLi a derivative of nor-cerpegin in dystrophic mdx mice." Thesis, Sorbonne université, 2020. http://www.theses.fr/2020SORUS095.

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La myopathie myofibrillaire est une maladie neuromusculaire à évolution lente caractérisée par de graves troubles musculaires causés par des mutations dans le gène codant pour des protéines du cytosquelette. L'un des gènes affectés en relation avec le développement de la MFM est DES. Des mutations dans le gène de la desmine entraînent des myopathies des muscles squelettiques et cardiaques. Cependant, les évènements qu'elles entraînent et qui sont à l’origine des phénotypes pathologiques cardiaques restent mal connus. Mon objectif est de créer un modèle in vitro de MFM basé sur des cellules sou

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9

Gerardo, Heloísa Salguinho. "Modulation of induced pluripotency by substrate rigidity." Master's thesis, 2014. http://hdl.handle.net/10316/31330.

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Dissertação de mestrado em Biologia Celular e Molecular, apresentada ao Departamento de Ciências da Vida da Faculdade de Ciências e Tecnologia da Universidade de Coimbra.<br>As células estaminais mesenquimais (MSCs) são células estaminais adultas, multipotentes, capazes de se auto renovar e diferenciar em diferentes tipos celulares dentro das linhagens de origem mesenquimal. A colheita de células estaminais mesenquimais é feita a partir de tecidos mesenquimais e também de tecidos extra embrionários. Estes últimos constituem uma boa fonte de MSCs, sendo estas mais naïve e com maior po

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10

"Harnessing cell response to substrate rigidity for tissue engineering applications using novel substrates with patterned elasticity." Thesis, 2010. http://hdl.handle.net/1911/62067.

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Cell response to substrate rigidity is an emerging field with implications in processes ranging from embryological development to the pathogenesis of disease states such as cancer or fibrosis, in which changes in tissue mechanical properties may inform cellular behavior. It may also serve as a valuable tool in tissue engineering, where materials must be chosen to best influence desired cell phenotype. This thesis describes novel substrates with patterned mechanical properties and their effects on mesenchymal stem cell (MSC) and macrophage behavior. Though substrate rigidity has previously been

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11

De, Leo Sarah Elizabeth. "Human T cell response to substrate rigidity for design of improved expansion platform." Thesis, 2014. https://doi.org/10.7916/D83F4N6H.

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Cells have long been known to sense and respond to mechanical stimuli in their environment. In the adoptive immune system particularly, cells are highly specialized and responsible for detecting and eliminating pathogens from the body. T cell mechanosensing is a relatively new field that explores how force transmission in cell-cell interaction elicits both inter- and intra-cell signaling. Owing to recent advances in genetic manipulation of T cells, it has emerged as new tool in immunotherapy. We recently demonstrated human T cell activation in response to mechanical rigidity of surfaces presen

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12

Shao, Fang-Yu, and 邵方璵. "Involvement of Focal Adhesion Kinase in cell Adhesion Force on Different Substrate Rigidity." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/49193977224332704715.

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碩士<br>國立成功大學<br>醫學工程研究所碩博士班<br>96<br>Both biochemical and mechanical properties of the microenvironment are equally important for cell growth and functional expression. Cell adhesion not only plays a critical role in cell physiology functions including morphology, spreading, migration, and differentiation, but also a critical step in the tissue engineering approach for maintenance of tissue structure and cell integration of biomaterial. The adhesion between cell-ECM is affected by intracellular regulations and extracellular environment, and is strengthened through focal adhesions. The focal ad

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13

Wen-ChiChen and 陳玟綺. "The Effect of Surface Morphology and Substrate Rigidity on Normal and Cancer Cells Migration." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/12240831126112607848.

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碩士<br>國立成功大學<br>醫學工程研究所碩博士班<br>98<br>Most cells are anchorage dependent. They require a surface to attach in order to migrate, proliferate, and differentiate. It is known several chemical factors can dramatically affect those functions of cells. Nevertheless, physical factors like stiffness and surface morphology of the substrata are also thought to influence cell behaviors. The purpose of this study was to measure the cell migration under different stiffness and structure of the substrata. Human melanoma cells (A2058) and human epidermal melanocytes (HEMn) were used for this study. The polydi

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14

Hu, Mufeng. "Biomaterial-based Cell Culture Platform for Podocyte Phenotype Study with Shape and Substrate Rigidity Control." Thesis, 2016. https://doi.org/10.7916/D8930TFM.

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Cells sense and interact with their microenvironment to retrieve signals which include cell-matrix and cell-cell contacts. These signals account for the influence of culturing conditions and often control the local cellular phenotype and global functions of tissues. Here, I sought to understand if there is any information processed by cells in guiding cellular phenotype given the control of cell shapes and substrate rigidities. If there is, would these phenotypic changes achieve biomedical purposes? What is the strategy to engineer platforms that can handle the longstanding challenges in those

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15

Chander, Ashok Coil. "Integrin-Linked Kinase, ECM Composition, and Substrate Rigidity Regulate Focal Adhesion - Actin Coupling, Modulating Survival, Proliferation and Migration: Towards a Biophysical Cancer Biomarker." Thesis, 2012. https://doi.org/10.7916/D8ZW1SVS.

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The extracellular matrix (ECM) has been implicated in numerous physiological and pathogenic processes. Integrins are thought to be the primary receptors that cells use to transduce biochemical and physical signals from the ECM. Integrin - ligand binding is specific for ECM molecules and is regulated by specific protein-protein interactions that further regulate downstream cellular activity such as motility, survival, growth, and proliferation. Termed outside-in signaling, the engagement of integrins results in protein recruitment to sites of cell - ECM contacts known as focal adhesions. Focal

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16

Shu, Shao-Jung, and 許韶容. "Substratum Rigidity Effects on Quantitative Cell Adhesion of Epithelial Cells and Fibroblasts." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/28688074740764679724.

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碩士<br>國立成功大學<br>醫學工程研究所碩博士班<br>95<br>Cell adhesion plays a critical role in cell physiology functions, which influences morphology, spreading, migration, and differentiation, etc. The adhesion is affected by intracellular regulations and extracellular environment. Most of the adhesion to extracellular matrix is derived from focal adhesions, which enhance adhesion, functioning as structural links between the extracellular matrix and the cytoskeleton that regulates intracellular tension, and direct cell function by triggering signaling pathways. 　In this study, the relationship between different

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17

Chang, Teng-kai, and 張登凱. "Measurement of cell detaching force on substrates with different rigidity by atomic force microscopy." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/92598148477067620248.

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碩士<br>國立成功大學<br>醫學工程研究所碩博士班<br>96<br>The cell can link another cell and ECM by adhesion molecules. The behavior of cell adhesion is influenced by the cell physiology and external environment. It plays an important role in cell morphology, motility, movement and differentiation, and cancer metastasis etc. So the ability to cell adhesion means that the cell carries out the normal physiological behavior. At present, a lot of technology can be used to quantify cell detaching force, for instance, micropipette, optical tweezers and so on. AFM has a lot of advantages, like parameters control easily,

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18

Yen-Chen, Hu, and 胡燕真. "Low substratum rigidity induces ubiquitin ligase Cul1 mediated-c-Jun degradation in nucleus of epithelial cells." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/71278981631236278819.

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碩士<br>國立成功大學<br>生理學研究所<br>94<br>Abstract The proto-oncoprotein c-Jun is a component of the transcription factor AP-1 (activator protein-1) involved in cellular proliferation, differentiation and death. Maintenance of c-Jun protein levels plays an important role in proliferation and survival of epithelial cells. Previous studies in our lab showed that epithelial cells cultured on collagen gel developed apoptosis due to low substratum rigidity. Low rigidity-induced cell apoptosis was mediated by degradation of c-Jun, which was observed only in epithelial, but not transformed cells. The purpose o

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19

Wei, Wei-Chun, and 魏瑋群. "Mechanosensing machinery and its mode of action for cells at substratum rigidity of soft tissue range." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/25308421658026871834.

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博士<br>國立成功大學<br>基礎醫學研究所<br>96<br>Most organs are soft, and the likely reason is that substratum rigidity plays important roles in regulation of cellular functions and behaviors. Mechanical stimuli are essential during development and tumorigenesis. However, how cells sense their physical environment has not been fully explored. The aim of this thesis is to understand how cells sense their physical environment. Two parts in this study are included: (1) to investigate whether and how low substratum rigidity of collagen gel modifies canonical integrin-mediated cellular behavior, and (2) to deline

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20

Huang, Yu-hsiang, and 黃煜翔. "Low substratum rigidity of polyacrylamide gel -induced epithelial cell apoptosis is mediated by degradation of JNK axis." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/17639616362737989347.

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碩士<br>國立成功大學<br>生理學研究所<br>96<br>Our previous studies have demonstrated that low-stiffness of collagen gel-induced epithelial cells apoptosis is mediated by deregulation of AP-1 proteins and endoplasmic reticulum stress-mediated disturbance of Ca2+ homeostasis. In order to elucidate to what extent and by what mechanism low rigidity induces apoptosis, we employed polyacrylamide (PA) gel to control substratum flexibility. We confirmed that low rigidity-induced apoptosis was only observed in epithelial cells. PA gel-induced apoptosis ratio was inversely correlated with substratum rigidity, and the

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21

Boudreau-Béland, Jonathan. "Effets de divers stimuli sur les caractéristiques des cardiomyocytes en culture dans le but de définir les conditions optimisées pour la fabrication de tissu cardiaque de remplacement." Thèse, 2015. http://hdl.handle.net/1866/18365.

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Encore en 2015, un grand nombre d’individus décèdent de pathologies du rythme cardiaque non contrôlées ou d’un manque de disponibilité de donneurs d’organes compatibles. Le génie tissulaire en créant, réparant ou améliorant la fonction des tissus est une option prometteuse afin de diminuer la mortalité associée à ces pathologies. L’objectif global de mon projet de recherche était de développer des outils et d’étudier l’impact fonctionnel des différents stimuli (mécanique et électrique) de l’environnement cardiaque dans le but de définir des conditions optimisées de culture pour la fabrication

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