To see the other types of publications on this topic, follow the link: Sugarcane yellow leaf virus.

Dissertations / Theses on the topic 'Sugarcane yellow leaf virus'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 32 dissertations / theses for your research on the topic 'Sugarcane yellow leaf virus.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.

1

Korimbocus, J., David Coates, I. Barker, and N. Boonham. "Improved detection of Sugarcane yellow leaf virus using a real- time fluorescent (TaqMan) RT-PCR assay." Elsevier, 2002. http://hdl.handle.net/10454/4037.

Full text
Abstract:
no<br>Yellow leaf syndrome (YLS) of sugarcane has been associated with Sugarcane yellow leaf virus (ScYLV) and has been reported from most sugarcane growing countries around the world. As sugarcane is vegetatively propagated, it is important to use effective and sensitive detection methods to screen new propagating material. Virus detection in symptomatic tissue is currently achieved using enzyme linked immunosorbent assay (ELISA), tissue blot immunoassay (TBIA) or a conventional RT-PCR based assay. This paper reports the development of an improved assay based on multiplex real-time fluorescen
APA, Harvard, Vancouver, ISO, and other styles
2

Burbano, Villavicencio Roberto Carlos. "Identificação de genótipos de Saccharum spp. resistentes ao amarelinho (Sugarcane yellow leaf virus) e ao mosaico (Sugarcane mosaic virus) e associação a marcadores moleculares /." Jaboticabal, 2019. http://hdl.handle.net/11449/183546.

Full text
Abstract:
Orientador: Luciana Rossini Pinto<br>Coorientador: Marcos Cesar Gonçalves<br>Banca: Dilermando Perecin<br>Banca: Paula Macedo Nobile<br>Banca: Antonio de Góes<br>Banca: Ivan Antônio dos Anjos<br>Resumo: O vírus do amarelinho (Sugarcane yellow leaf virus, SCYLV) e o vírus do mosaico (Sugarcane mosaic virus, SCMV) são duas importantes viroses que afetam os canaviais dos países produtores de cana-de-açúcar no mundo. As principais características da resistência a essas viroses, as metodologias de avaliação em campo, quantificação viral e as fontes de resistência foram estudadas neste trabalho. Par
APA, Harvard, Vancouver, ISO, and other styles
3

Cornish, Peter Verle. "Solution structure and functional analysis of a frameshift-stimulating RNA pseudoknot from sugarcane yellow leaf virus." Texas A&M University, 2005. http://hdl.handle.net/1969.1/3328.

Full text
Abstract:
Plant luteoviral RNA viruses employ -1 frameshifting for the production of P1 and P1-P2 fusion proteins important for viral replication. Luteoviral pseudoknots are characterized by three adenosines in the 3' side of loop L2 known to be important for maintaining frameshifting efficiency and pseudoknot stability. A proposed P1-P2 mRNA pseudoknot from sugarcane yellow leaf virus (ScYLV) was of interest since it contained two adenosine to cytidine substitutions in L2. Functional analysis shows that the in vitro frameshifting efficiency is greater (~15%) than any other luteoviral pseudoknot. The NM
APA, Harvard, Vancouver, ISO, and other styles
4

Gonçalves, Marcos Cesar. "Caracterização molecular do Sugarcane yellow leaf virus, desenvolvimento de um metodo de diagnostico altamente sensivel e aspectos moleculares da interação luteovirus/vetor." [s.n.], 2001. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315200.

Full text
Abstract:
Orientador: Jorge Vega<br>Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-07-31T18:41:28Z (GMT). No. of bitstreams: 1 Goncalves_MarcosCesar_D.pdf: 7477372 bytes, checksum: 67894da464a7b2387385005c386575c0 (MD5) Previous issue date: 2001<br>Resumo: O vírus do amarelecimento foliar da cana-de-açúcar, Sugarcane yellow leaf vírus (ScYL V) constitui atualmente um grande problema nos principais países produtores de cana-de-açúcar. Este vírus possui diversas características físicas e biológicas, como tamanho e morfologia da partícula,
APA, Harvard, Vancouver, ISO, and other styles
5

Rodrigues, Mariana Pelegrini [UNESP]. "Avaliação de resistência de cultivares de cana-de-açúcar ao Sugarcane yellow leaf virus (SCYLV) e ao seu afídeo vetor Melanaphis sacchari." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/131909.

Full text
Abstract:
Made available in DSpace on 2015-12-10T14:22:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-05-21. Added 1 bitstream(s) on 2015-12-10T14:28:37Z : No. of bitstreams: 1 000855237.pdf: 1808064 bytes, checksum: fcc5213095846f3cb1af0f6237332f31 (MD5)<br>Este trabalho teve como tema a avaliação da resistência de cultivares de cana-de-açúcar (Saccharum sp.) ao vírus do amarelecimento foliar (SCYLV) e ao seu principal afídeo vetor Melanaphis sacchari (Zehntner). Cinco cultivares de cana-de-açúcar (IACSP93 3046, IACSP95 5000, IACSP95 5094, IACSP96 3076 e SP71 6163) escolhidas devido à impo
APA, Harvard, Vancouver, ISO, and other styles
6

Rodrigues, Mariana Pelegrini. "Avaliação de resistência de cultivares de cana-de-açúcar ao Sugarcane yellow leaf virus (SCYLV) e ao seu afídeo vetor Melanaphis sacchari /." Jaboticabal, 2015. http://hdl.handle.net/11449/131909.

Full text
Abstract:
Orientador: Luciana Rossini Pinto<br>Coorientador: Marcos Cesar Gonçalves<br>Banca: Gustavo Vitti Môro<br>Banca: Fernando Javier Sanhueza Salas<br>Resumo: Este trabalho teve como tema a avaliação da resistência de cultivares de cana-de-açúcar (Saccharum sp.) ao vírus do amarelecimento foliar (SCYLV) e ao seu principal afídeo vetor Melanaphis sacchari (Zehntner). Cinco cultivares de cana-de-açúcar (IACSP93 3046, IACSP95 5000, IACSP95 5094, IACSP96 3076 e SP71 6163) escolhidas devido à importância econômica para os canaviais brasileiros, foram multiplicadas por cultura de meristema e indexadas e
APA, Harvard, Vancouver, ISO, and other styles
7

Abu, Ahmad Youssef. "Diversité génétique et variabilité du pouvoir pathogène du Sugarcane yellow leaf virus, agent causal de la feuille jaune de la canne à sucre." Montpellier, ENSA, 2006. http://www.theses.fr/2006ENSA0016.

Full text
Abstract:
Le Sugarcane yellow leaf virus (SCYLV) est un Polerovirus de la famille des Luteoviridae et l’agent causal de la feuille jaune, une maladie émergente de la canne à sucre. Quatre génotypes du SCYLV (BRA pour Brésil, CUB pour Cuba, PER pour Pérou et REU pour Réunion) ont été identifiés grâce à l’analyse phylogénétique du génome de 14 isolats viraux originaires du Brésil, de Chine, de Colombie, de Cuba, de Floride, d’Inde et de La Réunion. Des amorces spécifiques ont été dessinées pour différencier par RT-PCR ces génotypes et un fragment unique a été amplifié à partir de chacun d’entre eux, à l’e
APA, Harvard, Vancouver, ISO, and other styles
8

Débibakas, Sarah. "Impact de la diversité génétique du Sugarcane yellow leaf virus (SCYLV) sur les déterminismes de résistance de la canne à sucre à la feuille jaune." Thesis, Antilles-Guyane, 2012. http://www.theses.fr/2012AGUY0554.

Full text
Abstract:
Les variétés modernes de canne à sucre sont d'origine bispécifique et possèdent une structure génétique complexe, aneuploïde et hautement polyploïde rendant difficile les études de résistance génétique. La feuille jaune de la canne a sucre est une maladie dont l'agent causal est le sugarcane yellow leaf virus (scylv). Ce virus a une large diversité. Seuls trois génotypes viraux, différenciables par rtpcr, ont été trouves en Guadeloupe. Les objectifs de l'étude sont d'évaluer: l/la possibilité de marquer la résistance de la plante au scylv grâce a une étude d'association pan-génomique 2/l'impac
APA, Harvard, Vancouver, ISO, and other styles
9

Rassaby, Laurence. "Le syndrome de la feuille jaune de la canne à sucre causé par le Sugarcane yellow leaf virus (SCYLV) à la Réunion : caractérisation et impact." La Réunion, 2001. http://elgebar.univ-reunion.fr/login?url=http://thesesenligne.univ.run/01_15_Rassaby.pdf.

Full text
Abstract:
Des travaux ont été menés à la Réunion sur la syndrome de la feuille jaune (YLS) causé par le 'sugarcane yellow leaf virus' (SCYLV). Les caractéristiques agronomiques de cannes à sucre infectées ou non ont été comparées avec trois cultivars de canne à sucre : R570, R577 et R579. Des pertes de la production en tonnage (23%) et en sucre (11%) ont été mises en évidence uniquement pour le cultivar R577. Une enquête a été conduite pour déterminer la progression du YLS à la Réunion. Les taux d'infection ont varié selon le cultivar et selon le site de prélèvement, mais sont restés relativement stable
APA, Harvard, Vancouver, ISO, and other styles
10

Dang, Thi Van. "Tomato yellow leaf curl virus resistance in Solanum lycopersicum through transgenic approaches." Hannover Technische Informationsbibliothek und Universitätsbibliothek Hannover, 2009. http://d-nb.info/1003999433/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
11

Dang, Thi-Van [Verfasser]. "Tomato yellow leaf curl virus resistance in Solanum lycopersicum through transgenic approaches / Thi Van Dang." Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover, 2009. http://d-nb.info/1003999433/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
12

Conflon, Deborah. "Evaluation de la capacité du Tomato yellow leaf curl virus à maintenir des ADNs satellites." Thesis, Montpellier, SupAgro, 2015. http://www.theses.fr/2015NSAM0037/document.

Full text
Abstract:
Les virus du genre Begomovirus (famille Geminiviridae) sont fréquemment détectés en association avec des ADN satellites appelées alphasatellite et betasatellite qui font la moitié de la taille du génome viral. L’alphasatellite est autonome pour sa réplication et dépend du virus pour son mouvement et son encapsidation tandis que le betasatellite est dépendant de ces fonctions virales. L’alphasatellite a rarement été montré comme ayant un impact sur le virus assistant, contrairement au betasatellite qui augmente la virulence de son virus assistant. En dehors des bégomovirus tels que le Cotton le
APA, Harvard, Vancouver, ISO, and other styles
13

Kollenberg, Mario [Verfasser], and Dieter [Akademischer Betreuer] Jahn. "Differentielle Übertragung der Begomoviren Watermelon chlorotic stunt virus und Tomato yellow leaf curl virus durch Populationen der Weiße Fliege (Bemisia tabaci) / Mario Kollenberg ; Betreuer: Dieter Jahn." Braunschweig : Technische Universität Braunschweig, 2013. http://d-nb.info/1175822612/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
14

Maluta, Nathalie Kristine Prado. "Efeitos direto e indireto dos begomovírus Tomato severe rugose virus (ToSRV) e Tomato yellow leaf curl virus (TYLCV) no desempenho biológico de Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/11/11146/tde-25032013-142604/.

Full text
Abstract:
Sabe-se que a grande maioria dos fitopatógenos depende quase que exclusivamente de vetores para disseminarem-se para novos hospedeiros, porém pouco foi estudado no que se refere aos efeitos dos micro-organismos sobre seus insetos vetores. Sendo Bemisia tabaci uma praga de elevada importância e vetora de inúmeros vírus para plantas cultiváveis, é de extrema relevância estudar os efeitos provocados pelos vírus sobre seu desempenho biológico. Assim, esta pesquisa objetivou: a) avaliar os efeitos direto e indireto dos begomovírus Tomato severe rugose vírus (ToSRV) e Tomato yellow leaf curl virus (
APA, Harvard, Vancouver, ISO, and other styles
15

Kashima, Takayuki. "Studies on Biological Properties of a Novel Repellent, Acetylated Glyceride, against Adult Sweet potato Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)." Kyoto University, 2016. http://hdl.handle.net/2433/215228.

Full text
Abstract:
Rights to some articles appeared in this disseration are reserved by the ELSEVIER B.V., John Wiley & Sons, Inc. and Pesticide Science Society of Japan.If any of these materials you wish to use, authorisation from sources must be obtain.<br>Kyoto University (京都大学)<br>0048<br>新制・論文博士<br>博士(農学)<br>乙第13023号<br>論農博第2833号<br>新制||農||1043(附属図書館)<br>学位論文||H28||N4968(農学部図書室)<br>32951<br>(主査)教授 佐久間 正幸, 教授 松浦 健二, 教授 奥野 哲郎<br>学位規則第4条第2項該当
APA, Harvard, Vancouver, ISO, and other styles
16

Coates, David, C. Danks, J. Korimbocus, S. Preston, N. Boonham, and I. Barker. "Production of monoclonal antibodies to sugarcane yellow leaf virus using recombinant read-through protein." 2009. http://hdl.handle.net/10454/3088.

Full text
Abstract:
No<br>Yellow leaf syndrome (YLS) of sugarcane is associated with sugarcane yellow leaf virus (SCYLV), a member of the family Luteoviridae. A fragment of the coat protein and readthrough domain of SCYLV was expressed in a bacterial expression system. The resulting protein was purified and used to immunize mice for monoclonal antibody (MAb) production. Two MAbs, 3A2E3 and 2F7H5, were selected following the screening of hybridoma cells using both plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) and tissue blot immunoassay (TBIA). These MAbs can be incorporated into the TBIA ass
APA, Harvard, Vancouver, ISO, and other styles
17

Sayed, Abdelaleim Ismail Ibrahim el [Verfasser]. "Molecular characterization of sugarcane yellow leaf virus (SCYLV) and its effect on sucrose transporters in sugarcane Saccharum spp. hybrids / vorgelegt von Abdelaleim Ismail Ibrahim ElSayed." 2010. http://d-nb.info/1005345260/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
18

"Caracterização molecular do Sugarcane yellow leaf virus, desenvolvimento de um metodo de diagnostico altamente sensivel e aspectos moleculares da interação luteovirus/vetor." Tese, Biblioteca Digital da Unicamp, 2002. http://libdigi.unicamp.br/document/?code=vtls000239019.

Full text
APA, Harvard, Vancouver, ISO, and other styles
19

Debibakas, Sarah. "Impact de la diversité génétique du Sugarcane yellow leaf virus (SCYLV) sur les déterminismes de résistance de la canne à sucre à la feuille jaune." Thesis, 2012. http://www.theses.fr/2012AGUY0554/document.

Full text
Abstract:
Les variétés modernes de canne à sucre sont d'origine bispécifique et possèdent une structure génétique complexe, aneuploïde et hautement polyploïde rendant difficile les études de résistance génétique. La feuille jaune de la canne a sucre est une maladie dont l'agent causal est le sugarcane yellow leaf virus (scylv). Ce virus a une large diversité. Seuls trois génotypes viraux, différenciables par rtpcr, ont été trouves en Guadeloupe. Les objectifs de l'étude sont d'évaluer: l/la possibilité de marquer la résistance de la plante au scylv grâce a une étude d'association pan-génomique 2/l'impac
APA, Harvard, Vancouver, ISO, and other styles
20

Pérez, de Castro Ana María. "Mejora genética para la resistencia a los geminivirus tomato yellow leaf curl virus (TYLCV) y tomato yellow leaf curl sardinia virus (TYLCSV) en tomate." Doctoral thesis, 2020. http://hdl.handle.net/10251/135825.

Full text
Abstract:
[EN] Tomato yellow leaf curl disease (TYLCD) causes great damage in tomato (Solanum lycopersicum L.) crops in south-eastern Spain and in many tropical and subtropical areas in the world. The disease is caused by a complex of viruses, all belonging to the genus Begomovirus, family Geminiviridae. Nine species have been reported causing TYLCD and six more have been proposed as tentative species. Four viral species associated with TYLCD are present in Spain. Preventive measures to fight the disease, as well as measures based on controlling the insect vector (Bemisia tabaci Gen.) are not effe
APA, Harvard, Vancouver, ISO, and other styles
21

Tseng, Chien-Hao, and 曾建豪. "Recombinant Coat Protein Suppress Whitefly Transmission of Tomato yellow leaf curl Thailand virus." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/77905235285662905875.

Full text
Abstract:
碩士<br>國立臺灣大學<br>昆蟲學研究所<br>103<br>Whiteflies not only damage plants through feeding but also serve as vectors transmitting plant viruses. Whitefly-transmitted begomoviruses threaten a wide range of crops worldwide. Tomato yellow leaf curl Thailand virus (TYLCTHV) and Tomato leaf curl Taiwan virus (ToLCTWV) are two most prevalent viruses in tomato fields in Taiwan. Coat protein (CP), the only structural protein of begomoviruses, is hypothesized to interact with a putative receptor in the midgut of Bemisia tabaci. In vivo binding assay demonstrated recombinant TYLCTHV CP specifically bound to the
APA, Harvard, Vancouver, ISO, and other styles
22

Knierim, Dennis [Verfasser]. "Molecular characterization of Capsicum chlorosis virus, Tomato yellow leaf curl Thailand virus, Tobacco leaf curl Thailand virus and RNA-mediated virus resistance in Nicotiana benthamiana Domin. / von Dennis Knierim." 2007. http://d-nb.info/984383158/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
23

Hsieh, Chien-Kuei, and 謝芊桂. "In planta subcellular localizations and interactions of Tomato yellow leaf curl Thailand virus proteins." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/z53cxb.

Full text
Abstract:
碩士<br>國立臺灣大學<br>昆蟲學研究所<br>105<br>Tomato yellow leaf curl Thailand virus (TYLCTHV) is a member of the genus Begomovirus in the family Geminiviridae, and it is associated with tomato yellow leaf curl disease (TYLCD) in China, Myanmar, Thailand, and Taiwan. TYLCD is one of the major constraint on tomato production worldwide and often leads to 20-100% reduction in the yield of tomato particularly in tropical and subtropical areas. The genome of TYLCTHV is composed of bipartite DNA that are designated DNA-A and DNA-B. DNA-A carries six genes that encode the CP, the AV2 protein, the Rep protein, the
APA, Harvard, Vancouver, ISO, and other styles
24

Kungwon, Panadda, and 潘娜達. "Transformation and resistance evaluation of transgenic Nicotiana benthamiana resistant to Tomato yellow leaf curl Thailand virus and Tomato spotted wilt virus." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/68047968285760914871.

Full text
Abstract:
碩士<br>國立中興大學<br>國際農學碩士學位學程<br>104<br>Plant virus is one of the pathogens that can cause severe disease in agricultural crops worldwide. Tomato spotted wilt virus (TSWV) (genus Tospovirus) and Tomato yellow leaf curl Thailand virus (TYLCTHV) (genus Begomovirus) belong to the top ten economically important plant viruses. They can cause severe damage in many plants such as maize, sweet potato, rice, wheat, and most especially tomato, which is the most exported fruit in many countries. TSWV, a wide host range virus, was first reported in sweet pepper in Taiwan in 2009, which showed chlorosis and c
APA, Harvard, Vancouver, ISO, and other styles
25

Safarnejad, Mohammad Reza [Verfasser]. "Development of recombinant antibody mediated resistance against tomato yellow leaf curl virus / vorgelegt von Mohammad Reza Safarnejad." 2008. http://d-nb.info/987860003/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
26

Lin, Yu-Heng, and 林煜恆. "The study on antioxidation and proteomics of tomato plants infected by Tomato yellow leaf curl Thailand virus." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/54346816154032221045.

Full text
Abstract:
碩士<br>國立臺灣大學<br>園藝學研究所<br>99<br>Tomato yellow leaf curl disease (TYLCD) induced by Tomato yellow leaf curl virus is one of the most severe diseases of tomato. TYLCD defeats tomato production worldwide and often causes 100% economic loss. There is no efficient method to control TYLCD, except for breeding resistant cultivars. However, the plant physiology concerning TYLCD infection remains totally unknown. This research studied the antioxidation and proteomics of tomato plants after infection of TYLCD to understand the resistance mechanism. The resistant tomato line CLN2777G-1-5 and susceptible
APA, Harvard, Vancouver, ISO, and other styles
27

Huang, Yan-Hua, and 黃彥華. "The interaction between replication-associated protein (Rep) and viral genomic DNA of Tomato yellow leaf curl virus." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/70478466795679822881.

Full text
Abstract:
碩士<br>國立中興大學<br>生物科技學研究所<br>98<br>Tomato Yellow Leaf Curl Virus (TYLCV), a member of the family Geminiviridae, genus Begomovirus, harbors single-stranded circular DNA genome. The intergenic region of TYLCV genomic DNA contains bidirectional promoter elements interspersed within the origin of replication. The replication-associated protein (Rep) is essential for viral DNA replication, in which the Rep protein initiates rolling circle replication by binding to specific repeated sequences (iterons) within the origin of replication and acting as a topoisomerase. The aim of this study is to explore
APA, Harvard, Vancouver, ISO, and other styles
28

Kang, Yun-Ting, and 康昀婷. "Study of the point mutations on movement protein genes for mechanical transmissibility of tomato yellow leaf curl Thailand virus." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/6y8a7v.

Full text
Abstract:
碩士<br>國立中興大學<br>植物病理學系所<br>106<br>Geminiviridae is the largest family of plant viruses. The genus Begomovirus, which is the most important genus of geminiviridae, has one or two circular single stranded- DNA genome. Begomoviruses are usually transmitted by whitefly (Bemisia tabaci). Very few begomoviruses are mechanically transmissible by sap-inoculation on leaves. Previous studies in our lab have shown that tomato leaf curl New Delhi virus oriental melon isolate (ToLCNDV-OM) is an isolate of bipartite begomovirus and can infect cucurbits via mechanical transmission. Genome reassortment assay
APA, Harvard, Vancouver, ISO, and other styles
29

Tsai, Yu-Ting, and 蔡玉婷. "Studies on the molecular mechanism for modulation of leaf curling symptoms by C4 protein of Ageratum yellow vein virus." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/82437134191017235214.

Full text
Abstract:
碩士<br>國立中興大學<br>生物科技學研究所<br>102<br>Plants infected by geminiviruses usually show typical leaf curling symptoms with severe growth retardation and yield reduction, leading to serious damages to agricultural economy. Specific geminivirus species could inflict leaf curling towards unique direction on a specific host. Previous studies in our lab have revealed that the C4 proteins of geminiviruses are involved in the determination of the directions of leaf curling symptoms. However, the underlying mechanisms remained to be explored. Therefore the objectives of this study are to identify the key ami
APA, Harvard, Vancouver, ISO, and other styles
30

Hernandez, Edith Sanchez. "Layered double hydroxide (LDH)-mediated topical delivery of dsRNA for protection against Tomato yellow leaf curl virus (TYLCV) in Nicotiana benthamiana." Thesis, 2021. http://hdl.handle.net/10754/668987.

Full text
Abstract:
Cell wall is the major barrier in the delivery of biomolecules such as nucleic acids into the plant cell. Biological (bacteria or viruses) and biolistic (particle-based) methods are used to deliver nucleic acids into the plant cell. However, these methods have significant limitations when it comes to species range, scalability, and field assays. In this work, we report the use of layered double hydroxide (LDH) topically applied to deliver RNA molecules into the plant cell. LDH were assembled by methanol-based co-precipitation of magnesium and aluminum nitrate solution with sodium hydroxide and
APA, Harvard, Vancouver, ISO, and other styles
31

Chen, Chung-Lin, and 陳俊麟. "The Construction of Infectious Tomato Yellow Leaf Curl Virus(TYLCV) and the Analysis of Resistance on Transgenic Tobacco with TYLCV Replicase Gene." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/37443994317159114605.

Full text
Abstract:
碩士<br>大葉大學<br>分子生物科技學系碩士班<br>95<br>Tomato yellow leaf curl virus(TYLCV) is a member of the Begomovirus in the Geminiviridae family. TYLCV contains a monopartite genome and is transmitted by whitefly Bemisia argentifoli and results in severe losses in tomato productions. In this study, naturally infected tomato samples were collected from eight tomato fields in Changhua County and polymerase chain reaction (PCR) were used for the detection of TYLCV using primers pair that is specific to TYLCV genome. Five in the forty samples from Sihu and one in eight samples collected from Yongjin, Dacun,
APA, Harvard, Vancouver, ISO, and other styles
32

Chen, Shu-Jun, and 陳淑君. "Development of techniques for quick and easy RNA extraction for virus detection and molecular identification of yellow leaf pathogens of phalaenopsis orchids." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/69260273304731986004.

Full text
Abstract:
碩士<br>朝陽科技大學<br>生物技術研究所<br>97<br>Phalaenopsis is currently the most important exporting item for the ornamental industry in Taiwan. In recent years, companies from the Netherlands have been taking advantage of their virus-free planting materials propagation system to promote their phalaenopsis; that apparently has threaten the competitiveness of Taiwan’s orchid industry in the international market. Therefore, improving the quality and virus control status of our phalaenopsis products has become the key issue for maintaining the competitiveness of our orchid industry, and for this reason, a de
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!