Academic literature on the topic 'Super-resolution ; dSTORM ; fluorescence microscopy'

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Journal articles on the topic "Super-resolution ; dSTORM ; fluorescence microscopy"

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Whelan, Donna R., Thorge Holm, Markus Sauer, and Toby D. M. Bell. "Focus on Super-Resolution Imaging with Direct Stochastic Optical Reconstruction Microscopy (dSTORM)." Australian Journal of Chemistry 67, no. 2 (2014): 179. http://dx.doi.org/10.1071/ch13499.

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The last decade has seen the development of several microscopic techniques capable of achieving spatial resolutions that are well below the diffraction limit of light. These techniques, collectively referred to as ‘super-resolution’ microscopy, are now finding wide use, particularly in cell biology, routinely generating fluorescence images with resolutions in the order of tens of nanometres. In this highlight, we focus on direct Stochastic Optical Reconstruction Microscopy or dSTORM, one of the localisation super-resolution fluorescence microscopy techniques that are founded on the detection o
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Khan, Abdullah O., Alessandro Di Maio, Emily J. Guggenheim, et al. "Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO2 Nanomaterials Following Uptake and Sub-Cellular Localization." Nanomaterials 10, no. 3 (2020): 401. http://dx.doi.org/10.3390/nano10030401.

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Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of different surface modifications (PVP, Dispex AA4040, and Pluronic F127) on the uptake, cellular distribution, and degradation of titanium dioxide NMs (TiO2 NMs, ~10 nm core size) are assessed and correlated with the localization of fluorescently-labeled serum proteins forming their coronas. Imaging appr
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Danson, Amy E., Alex McStea, Lin Wang, et al. "Super-Resolution Fluorescence Microscopy Reveals Clustering Behaviour of Chlamydia pneumoniae’s Major Outer Membrane Protein." Biology 9, no. 10 (2020): 344. http://dx.doi.org/10.3390/biology9100344.

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Chlamydia pneumoniae is a Gram-negative bacterium responsible for a number of human respiratory diseases and linked to some chronic inflammatory diseases. The major outer membrane protein (MOMP) of Chlamydia is a conserved immunologically dominant protein located in the outer membrane, which, together with its surface exposure and abundance, has led to MOMP being the main focus for vaccine and antimicrobial studies in recent decades. MOMP has a major role in the chlamydial outer membrane complex through the formation of intermolecular disulphide bonds, although the exact interactions formed ar
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Jayasinghe, Isuru D., Michelle Munro, David Baddeley, Bradley S. Launikonis, and Christian Soeller. "Observation of the molecular organization of calcium release sites in fast- and slow-twitch skeletal muscle with nanoscale imaging." Journal of The Royal Society Interface 11, no. 99 (2014): 20140570. http://dx.doi.org/10.1098/rsif.2014.0570.

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Localization microscopy is a fairly recently introduced super-resolution fluorescence imaging modality capable of achieving nanometre-scale resolution. We have applied the dSTORM variation of this method to image intracellular molecular assemblies in skeletal muscle fibres which are large cells that critically rely on nanoscale signalling domains, the triads. Immunofluorescence staining in fixed adult rat skeletal muscle sections revealed clear differences between fast- and slow-twitch fibres in the molecular organization of ryanodine receptors (RyRs; the primary calcium release channels) with
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Jing, Yingying, Mingjun Cai, Haijiao Xu, et al. "Aptamer-recognized carbohydrates on the cell membrane revealed by super-resolution microscopy." Nanoscale 10, no. 16 (2018): 7457–64. http://dx.doi.org/10.1039/c8nr00089a.

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Huang, Bo, Mark Bates, and Xiaowei Zhuang. "Super-Resolution Fluorescence Microscopy." Annual Review of Biochemistry 78, no. 1 (2009): 993–1016. http://dx.doi.org/10.1146/annurev.biochem.77.061906.092014.

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Garcia-Guerrero, Estefania, Luis Gerardo Rodríguez-Lobato, Sophia Danhof, et al. "ATRA Augments BCMA Expression on Myeloma Cells and Enhances Recognition By BCMA-CAR T-Cells." Blood 136, Supplement 1 (2020): 13–14. http://dx.doi.org/10.1182/blood-2020-142572.

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Background: B cell maturation antigen (BCMA) is a B-lineage antigen that is retained on malignant plasma cells in multiple myeloma (MM), and is under investigation as a target antigen for humoral and cellular immunotherapy. Targeting BCMA with chimeric antigen receptor (CAR) T-cells, T-cell engaging antibodies and antibody-drug conjugates has resulted in high rates of clinical responses however, the depth and durability of these responses is still not satisfactory and most patients ultimately relapse. This has been attributed at least in part to low or non-uniform BCMA expression on MM cells,
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Varga, Dániel, Hajnalka Majoros, Zsuzsanna Ujfaludi, Miklós Erdélyi, and Tibor Pankotai. "Quantification of DNA damage induced repair focus formation via super-resolution dSTORM localization microscopy." Nanoscale 11, no. 30 (2019): 14226–36. http://dx.doi.org/10.1039/c9nr03696b.

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Gao, Jing, Ye Wang, Mingjun Cai, et al. "Mechanistic insights into EGFR membrane clustering revealed by super-resolution imaging." Nanoscale 7, no. 6 (2015): 2511–19. http://dx.doi.org/10.1039/c4nr04962d.

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We investigate the distribution of membrane EGFR by direct stochastic optical reconstruction microscopy (dSTORM). Our results illustrate the clustering distribution pattern of EGFR in polarized cells and uncover the essential role of lipid rafts in EGFR cluster maintenance.
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Zhanghao, Karl, Juntao Gao, Dayong Jin, Xuedian Zhang, and Peng Xi. "Super-resolution fluorescence polarization microscopy." Journal of Innovative Optical Health Sciences 11, no. 01 (2017): 1730002. http://dx.doi.org/10.1142/s1793545817300026.

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Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules. Several recent super resolution techniques have been applied to fluorescence polarization microscopy, achieving dipole measurement at nanoscale. In this review, we summarize both diffraction limited and super resolution fluorescence polarization microscopy techniques, as well as their applications in biological imaging.
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Dissertations / Theses on the topic "Super-resolution ; dSTORM ; fluorescence microscopy"

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Sanders, James Henry. "Direct stochastic optical reconstruction microscopy (dSTORM) imaging of cellular structures." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/direct-stochastic-optical-reconstruction-microscopy-dstorm-imaging-of-cellular-structures(915e2c88-c81a-4b24-ac53-6ab7ffcbf4d8).html.

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The diffraction limit restricts conventional light microscopes to approximately 250 nm laterally and 500 nm axially, these limits being first proposed by Abbe in 1873. Despite this, optical microscopes have found many applications in biological research and single cells that are 10 - 100 um in size. Furthermore by coupling the non-invasive nature of a light microscope with highly sensitive fluorescent probes, fluorescence microscopy has also become a standard imaging technique. Recent advances in fluorescence microscopy now provide a number of methods to circumvent the Abbe diffraction limit,
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Glushonkov, Oleksandr. "Imagerie de fluorescence à haute résolution : étude de la localisation nucléolaire de la protéine de la nucléocapside du VIH." Thesis, Strasbourg, 2018. http://www.theses.fr/2018STRAJ028/document.

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Au cours de ce travail de thèse expérimental, nous nous sommes intéressés à l’étude de la localisation nucléaire et nucléolaire de la protéine de la nucléocapside (NC) du VIH-1. Des études antérieures menées au laboratoire avaient mis en évidence une très forte accumulation de la NC dans les nucléoles. Ce compartiment nucléaire est connu pour être ciblé par de nombreux virus afin de promouvoir leur réplication. Des expériences de microscopie électronique avaient révélé la structure complexe du nucléole et montré qu’il est composé de trois sous-compartiments : les centres fibrillaires, le compa
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Valadés, Cruz César Augusto. "Polarized super-resolution fluorescence microscopy." Doctoral thesis, Universitat Politècnica de Catalunya, 2014. http://hdl.handle.net/10803/277565.

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While super-resolution microscopy has brought a significant improvement in nano-scale imaging of molecular assemblies in biological media, its extension to imaging molecular orientation using fluorescence anisotropy has not yet been fully explored. Providing orientational order information at the nano-scale would be of considerable interest for the understanding of biological functions since they are intrinsically related to structural fundamental processes such as in protein clustering in cell membranes, supra-molecular polymerization or aggregation. In this thesis, we propose a super-resolut
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Valadés, Cruz César Augusto. "Polarized super-resolution fluorescence microscopy." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4333.

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Alors que la microscopie super-résolue a apporté une amélioration considérable en imagerie des assemblages moléculaires dans les milieux biologiques à l'échelle nanométrique, son extension à l'imagerie de l'orientation moléculaire, utilisant l'anisotropie de fluorescence, n'a pas encore été complètement explorée. Apporter une information sur l'orientation moléculaire à l'échelle nanométrique aurait un intérêt considérable pour la compréhension des fonctions biologiques. Dans cette thèse, nous proposons une technique de microscopie super-résolution résolue en polarisation, capable d'imager les
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Mandula, Ondrej. "Super-resolution methods for fluorescence microscopy." Thesis, University of Edinburgh, 2013. http://hdl.handle.net/1842/8909.

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Fluorescence microscopy is an important tool for biological research. However, the resolution of a standard fluorescence microscope is limited by diffraction, which makes it difficult to observe small details of a specimen’s structure. We have developed two fluorescence microscopy methods that achieve resolution beyond the classical diffraction limit. The first method represents an extension of localisation microscopy. We used nonnegative matrix factorisation (NMF) to model a noisy dataset of highly overlapping fluorophores with intermittent intensities. We can recover images of individual sou
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Zhou, Zhaokun. "Magneto-optical tweezers with super-resolution fluorescence microscopy." Thesis, University of York, 2017. http://etheses.whiterose.ac.uk/18771/.

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This thesis describes the design, construction and application of a novel magneto-optical tweezers with super-resolution fluorescence microscopy for manipulation, force/torque measurement and imaging of single biomolecules. The optical tweezers component offers force or position clamping in three dimensions. The 3D-printed magnetic tweezers is designated for rotation in the vertical plane. The separation of rotation from force transduction results in the capability of precise torque measurement. The filamentous biomolecules to be used in the device will lie in a transverse direction in the ima
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Persson, Roger. "Breaking the diffraction limit using conical diffraction in super resolution fluorescence microscopy : Breaking the diffraction limit using conical diffraction in super resolution fluorescence microscopy." Thesis, KTH, Tillämpad fysik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-140725.

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Needham, Lisa-Maria. "Next-generation fluorophores for single-molecule and super-resolution fluorescence microscopy." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/283232.

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The development of single-molecule and super-resolution fluorescence techniques has revolutionised biological imaging. Nano-scale cellular structures and heterogeneous dynamic processes are now able to be visualised with unprecedented resolution in both time and space. The achievable localisation precision and therefore the resolution is fundamentally limited by the number of photons a single-fluorophore can emit. The ideal super-resolution dye would emit a large number of photons over a short period of time. On the contrary, an optimal single-molecule tracking probe would be highly photostabl
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Bento, Carvalho Almada Pedro Manuel. "Developing highly multiplexed technology for high-throughput super-resolution fluorescence microscopy." Thesis, University College London (University of London), 2018. http://discovery.ucl.ac.uk/10041569/.

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High-Throughput imaging can reconstruct complex signalling networks, reveal unknown interactions and capture rare cellular events. Simultaneously, the development of Single Molecule Localization Super Resolution Microscopy has enabled molecular-level structural information to be obtained in a single cell. But the increase in resolution comes at a trade-off for the amount of molecular species that can be imaged and the time it takes to acquire data, all of which limit the applicability of super-resolution to high-throughput work-flows. The present work details a framework to address this. It co
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Zwettler, Fabian Ulrich [Verfasser], and Markus [Gutachter] Sauer. "Expansion Microscopy combined with Super-Resolution Fluorescence Microscopy / Fabian Ulrich Zwettler ; Gutachter: Markus Sauer." Würzburg : Universität Würzburg, 2021. http://d-nb.info/122529584X/34.

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Books on the topic "Super-resolution ; dSTORM ; fluorescence microscopy"

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Fluorescence Microscopy: Super-Resolution and Other Novel Techniques. Elsevier Science & Technology Books, 2014.

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Book chapters on the topic "Super-resolution ; dSTORM ; fluorescence microscopy"

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Sauer, Markus, and Mike Heilemann. "Localization-Based Super-Resolution Microscopy." In Fluorescence Microscopy. Wiley-VCH Verlag GmbH & Co. KGaA, 2017. http://dx.doi.org/10.1002/9783527687732.ch8.

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Mondal, Partha Pratim, and Alberto Diaspro. "Super-resolution Fluorescence Microscopy." In Fundamentals of Fluorescence Microscopy. Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-007-7545-9_9.

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Birk, Udo, Gerrit Best, Roman Amberger, and Christoph Cremer. "Super-Resolution Microscopy: Interference and Pattern Techniques." In Fluorescence Microscopy. Wiley-VCH Verlag GmbH & Co. KGaA, 2017. http://dx.doi.org/10.1002/9783527687732.ch9.

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Best, Gerrit, Roman Amberger, and Christoph Cremer. "Super-Resolution Microscopy: Interference and Pattern Techniques." In Fluorescence Microscopy. Wiley-VCH Verlag GmbH & Co. KGaA, 2013. http://dx.doi.org/10.1002/9783527671595.ch9.

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Sauer, Markus. "A Practical Guide to dSTORM: Super-Resolution Imaging with Standard Fluorescent Probes." In Springer Series on Fluorescence. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/4243_2012_41.

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Wicker, Kai. "Super-Resolution Fluorescence Microscopy Using Structured Illumination." In Neuromethods. Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-983-3_7.

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Steinhauer, Christian, Michelle S. Itano, and Philip Tinnefeld. "Super-Resolution Fluorescence Imaging with Blink Microscopy." In Nanoimaging. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-137-0_8.

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Cremer, Gert De, Bert F. Sels, Dirk E. De Vos, Johan Hofkens, and Maarten B. J. Roeffaers. "NASCA Microscopy: Super-Resolution Mapping of Chemical Reaction Centers." In Springer Series on Fluorescence. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/4243_2011_33.

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Lampe, Marko, and Wernher Fouquet. "Requirements for Samples in Super-Resolution Fluorescence Microscopy." In Neuromethods. Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-983-3_14.

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Feng, Han, Xiaobo Wang, Zhiwei Xu, Xiaoju Zhang, and Yongju Gao. "Super-Resolution Fluorescence Microscopy for Single Cell Imaging." In Single Cell Biomedicine. Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-0502-3_6.

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Conference papers on the topic "Super-resolution ; dSTORM ; fluorescence microscopy"

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Varga, Dániel, Hajnalka Majoros, Zsuzsanna Ujfaludi, Tibor Pankotai, and Miklos Erdélyi. "Quantification of labelled target molecules via super-resolution dSTORM localization microscopy." In Single Molecule Spectroscopy and Superresolution Imaging XIII, edited by Ingo Gregor, Rainer Erdmann, and Felix Koberling. SPIE, 2020. http://dx.doi.org/10.1117/12.2545099.

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Zhanghao, Karl, Long Chen, Xusan Yang, et al. "Super-resolution fluorescence dipole orientation microscopy." In 2016 Progress in Electromagnetic Research Symposium (PIERS). IEEE, 2016. http://dx.doi.org/10.1109/piers.2016.7734948.

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Glogger, Marius. "Exchangeable fluorophore labels in super-resolution fluorescence microscopy." In European Light Microscopy Initiative 2021. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.elmi2021.86.

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Park, No-Cheol, Hyungbae Moon, Wonsup Lee, Geon Lim, Guk-Jong Choi, and Young-Pil Park. "SIL-STED microscopy technique enhancing super-resolution of fluorescence microscopy." In Optical Data Storage 2017: From New Materials to New Systems, edited by Ryuichi Katayama and Yuzuru Takashima. SPIE, 2017. http://dx.doi.org/10.1117/12.2275957.

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Kim, Sungho, Gyeong Tae Kim, Soohyun Jang, Sang-Hee Shim, and Sung Chul Bae. "Correlative super-resolution fluorescence microscopy combined with optical coherence microscopy." In SPIE BiOS, edited by Fred S. Azar and Xavier Intes. SPIE, 2015. http://dx.doi.org/10.1117/12.2079340.

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Guha, Aparajeeta, and Rajiv Ganguly. "Challenges and opportunities in super-resolution fluorescence microscopy." In 2017 4th International Conference on Opto-Electronics and Applied Optics (Optronix). IEEE, 2017. http://dx.doi.org/10.1109/optronix.2017.8349995.

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Heilemann, Mike. "Exchangeable fluorophore labels in super-resolution fluorescence microscopy." In Single Molecule Spectroscopy and Superresolution Imaging XIV, edited by Ingo Gregor, Rainer Erdmann, and Felix Koberling. SPIE, 2021. http://dx.doi.org/10.1117/12.2584988.

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Zhu, Ming-Qiang, Guo-Feng Zhang, Zhe Hu, Wen-Liang Gong, Matthew P. Aldred, and Zhen-Li Huang. "Biodegradable polymer nanoparticles with photoswitchable fluorescence for super-resolution bioimaging." In Novel Techniques in Microscopy. OSA, 2013. http://dx.doi.org/10.1364/ntm.2013.nm2b.7.

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Ilovitsh, Tali, Amihai Meiri, Zeev Zalevsky, et al. "Image processing for super-resolution localization in fluorescence microscopy." In 2013 12th Workshop on Information Optics (WIO). IEEE, 2013. http://dx.doi.org/10.1109/wio.2013.6601248.

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Guo, Min, Panagiotis Chandris, John P. Giannini, Jiji Chen, Harshad D. Vishwasrao, and Hari Shroff. "Combining Total Internal Reflection Fluorescence Microscopy with Rapid Super-resolution Imaging." In Novel Techniques in Microscopy. OSA, 2019. http://dx.doi.org/10.1364/ntm.2019.nw2c.4.

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Reports on the topic "Super-resolution ; dSTORM ; fluorescence microscopy"

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Santra, Kalyan. Acquisition and analysis of steady-state and time-resolved fluorescence data for applications in materials science, bioanalytical chemistry, and super-resolution microscopy. Office of Scientific and Technical Information (OSTI), 2018. http://dx.doi.org/10.2172/1505191.

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