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Journal articles on the topic 'Surface bacteria'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Hogan, Kayla, Sai Paul, Guanyou Lin, Jay Fuerte-Stone, Evgeni V. Sokurenko, and Wendy E. Thomas. "Effect of Gravity on Bacterial Adhesion to Heterogeneous Surfaces." Pathogens 12, no. 7 (2023): 941. http://dx.doi.org/10.3390/pathogens12070941.

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Bacterial adhesion is the first step in the formation of surface biofilms. The number of bacteria that bind to a surface from the solution depends on how many bacteria can reach the surface (bacterial transport) and the strength of interactions between bacterial adhesins and surface receptors (adhesivity). By using microfluidic channels and video microscopy as well as computational simulations, we investigated how the interplay between bacterial transport and adhesivity affects the number of the common human pathogen Escherichia coli that bind to heterogeneous surfaces with different receptor

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Patel, Nirav, Ryan Guillemette, Ratnesh Lal, and Farooq Azam. "Bacterial surface interactions with organic colloidal particles: Nanoscale hotspots of organic matter in the ocean." PLOS ONE 17, no. 8 (2022): e0272329. http://dx.doi.org/10.1371/journal.pone.0272329.

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Colloidal particles constitute a substantial fraction of organic matter in the global ocean and an abundant component of the organic matter interacting with bacterial surfaces. Using E. coli ribosomes as model colloidal particles, we applied high-resolution atomic force microscopy to probe bacterial surface interactions with organic colloids to investigate particle attachment and relevant surface features. We observed the formation of ribosome films associating with marine bacteria isolates and natural seawater assemblages, and that bacteria readily utilized the added ribosomes as growth subst

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Chen, Chao, and Monica Ek. "Antibacterial evaluation of CNF/PVAm multilayer modified cellulose fiber and cellulose model surface." Nordic Pulp & Paper Research Journal 33, no. 3 (2018): 385–96. http://dx.doi.org/10.1515/npprj-2018-3050.

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Abstract Earlier studies have shown that 3-layer-modified cellulose fibers with poly(acrylic acid) (PAA) as the middle layer between two cationic polyelectrolyte polyvinylamine (PVAm) layers have strong antibacterial efficacy in terms of both bacteria adsorption and bacterial growth inhibition. In the present work, the fossil-based PAA middle layer was replaced by sustainable wood-based cellulose nano-fibrils (CNF), i. e., the fibers were modified by a 3-layer PVAm/CNF/PVAm system. Interestingly, the antibacterial efficacy of this system was greater than that of the previous PVAm/PAA/PVAm syst

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Yang, Xiao, Wei Zhang, Xuezhi Qin, et al. "Recent Progress on Bioinspired Antibacterial Surfaces for Biomedical Application." Biomimetics 7, no. 3 (2022): 88. http://dx.doi.org/10.3390/biomimetics7030088.

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Surface bacterial fouling has become an urgent global challenge that calls for resilient solutions. Despite the effectiveness in combating bacterial invasion, antibiotics are susceptible to causing microbial antibiotic resistance that threatens human health and compromises the medication efficacy. In nature, many organisms have evolved a myriad of surfaces with specific physicochemical properties to combat bacteria in diverse environments, providing important inspirations for implementing bioinspired approaches. This review highlights representative natural antibacterial surfaces and discusses

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Absolom, Darryl R. "The role of bacterial hydrophobicity in infection: bacterial adhesion and phagocytic ingestion." Canadian Journal of Microbiology 34, no. 3 (1988): 287–98. http://dx.doi.org/10.1139/m88-054.

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The role that bacterial surface hydrophobicity (surface tension) plays in determining the extent of adhesion of polymer substrates and phagocytic ingestion is reviewed. The early attachment phase in bacterial adhesion is shown to depend critically on the relative surface tensions of the three interacting phases; i.e., bacteria, substrate, and suspending liquid surface tension. When suspended in a liquid with a high surface tension such as Hanks balanced salt solution, the most hydrophobic bacteria adhere to all surfaces to the greatest extent. When the liquid surface tension (γLV) is larger th

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Vadillo-Rodríguez, Virginia, Henk J. Busscher, Willem Norde, et al. "Comparison of Atomic Force Microscopy Interaction Forces between Bacteria and Silicon Nitride Substrata for Three Commonly Used Immobilization Methods." Applied and Environmental Microbiology 70, no. 9 (2004): 5441–46. http://dx.doi.org/10.1128/aem.70.9.5441-5446.2004.

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ABSTRACT Atomic force microscopy (AFM) has emerged as a powerful technique for mapping the surface morphology of biological specimens, including bacterial cells. Besides creating topographic images, AFM enables us to probe both physicochemical and mechanical properties of bacterial cell surfaces on a nanometer scale. For AFM, bacterial cells need to be firmly anchored to a substratum surface in order to withstand the friction forces from the silicon nitride tip. Different strategies for the immobilization of bacteria have been described in the literature. This paper compares AFM interaction fo

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Evans, Adele, Anthony J. Slate, Millie Tobin, et al. "Multifractal Analysis to Determine the Effect of Surface Topography on the Distribution, Density, Dispersion and Clustering of Differently Organised Coccal-Shaped Bacteria." Antibiotics 11, no. 5 (2022): 551. http://dx.doi.org/10.3390/antibiotics11050551.

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The topographic features of surfaces are known to affect bacterial retention on a surface, but the precise mechanisms of this phenomenon are little understood. Four coccal-shaped bacteria, Staphylococcus sciuri, Streptococcus pyogenes, Micrococcus luteus, and Staphylococcus aureus, that organise in different cellular groupings (grape-like clusters, tetrad-arranging clusters, short chains, and diploid arrangement, respectively) were used. These differently grouped cells were used to determine how surface topography affected their distribution, density, dispersion, and clustering when retained o

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Chiang, Wen-Chi, Casper Schroll, Lisbeth Rischel Hilbert, Per Møller, and Tim Tolker-Nielsen. "Silver-Palladium Surfaces Inhibit Biofilm Formation." Applied and Environmental Microbiology 75, no. 6 (2009): 1674–78. http://dx.doi.org/10.1128/aem.02274-08.

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ABSTRACT Undesired biofilm formation is a major concern in many areas. In the present study, we investigated biofilm-inhibiting properties of a silver-palladium surface that kills bacteria by generating microelectric fields and electrochemical redox processes. For evaluation of the biofilm inhibition efficacy and study of the biofilm inhibition mechanism, the silver-sensitive Escherichia coli J53 and the silver-resistant E. coli J53[pMG101] strains were used as model organisms, and batch and flow chamber setups were used as model systems. In the case of the silver-sensitive strain, the silver-

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Fang, F., J. Kennedy, M. Dhillon, and S. Flint. "Antibacterial effect of silver nanofilm modified stainless steel surface." International Journal of Modern Physics B 29, no. 10n11 (2015): 1540013. http://dx.doi.org/10.1142/s0217979215400135.

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Bacteria can attach to stainless steel surfaces, resulting in the colonization of the surface known as biofilms. The release of bacteria from biofilms can cause contamination of food such as dairy products in manufacturing plants. This study aimed to modify stainless steel surfaces with silver nanofilms and to examine the antibacterial effectiveness of the modified surface. Ion implantation was applied to produce silver nanofilms on stainless steel surfaces. 35 keV Ag ions were implanted with various fluences of 1 × 1015 to 1 × 1017 ions•cm-2 at room temperature. Representative atomic force mi

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Güngör, ö. E., Z. Kırzıoğlu, E. Dinçer, and M. Kıvanç. "Who will win the race in childrens' oral cavities? Streptococcus mutans or beneficial lactic acid bacteria?" Beneficial Microbes 4, no. 3 (2013): 237–45. http://dx.doi.org/10.3920/bm2012.0055.

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Adhesion to oral soft and hard tissue is crucial for bacterial colonisation in the mouth. The aim of this work was to select strains of oral lactic acid bacteria that could be used as probiotics for oral health. To this end, the adhesive properties of some lactic acid bacteria were investigated. Seventeen lactic acid bacteria including two Streptococcus mutans strains were isolated from the oral cavity of healthy children, while other strains were isolated from fermented meat products. The bacterial strains were applied to teeth surfaces covered with saliva or without saliva. A significant div

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Grudzień, Joanna, Magdalena Jarosz, Kamil Kamiński, et al. "Growth of Lactic Acid Bacteria on Gold—Influence of Surface Roughness and Chemical Composition." Nanomaterials 10, no. 12 (2020): 2499. http://dx.doi.org/10.3390/nano10122499.

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The main focus of this work was to establish a correlation between surface topography and chemistry and surface colonization by lactic acid bacteria. For this reason, we chose gold substrates with different surface architectures (i.e., smooth and nanorough) that were characterized by atomic force microscopy (AFM), electron scanning microscopy (SEM), and X-ray diffractometry (XRD). Moreover, to enhance biocompatibility, we modified gold substrates with polymeric monolayers, namely cationic dextran derivatives with different molar masses. The presence of those layers was confirmed by AFM, infrar

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Kelly, D., R. Begbie, A. J. Flanagan, R. G. Mattison, and T. P. King. "Interactions between bacteria and the surface of the small intestine." Proceedings of the British Society of Animal Production (1972) 1993 (March 1993): 31. http://dx.doi.org/10.1017/s0308229600023606.

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Many bacterial species are closely associated with epithelial surfaces of the gastrointestinal tract. The physicochemical interactions between bacteria and the intestinal surface are complex and include both stereospecific and chemotactic effects. Such mechanisms of interaction and attachment of bacteria to the gut have received considerable attention over the last decade as they have been recognised as an important initial event in colonisation and the pathogenesis of enteric infections. Many disease-causing bacteria possess surface fimbriae or fibrillae with lectin-like adhesins which intera

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Kotoulas, Nicholas K., Stephanie Tan, Justin R. Nodwell, and M. Cynthia Goh. "Differentiating bacteria by their unique surface interactions." PLOS One 20, no. 6 (2025): e0327489. https://doi.org/10.1371/journal.pone.0327489.

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New, rapid, and accessible approaches to bacterial detection are necessary to help curb the rising impacts of antimicrobial resistance. In this study, we introduce a technique that distinguishes bacteria through their unique surface interactions. By measuring and combining the interaction strengths of a bacterium across a set of chemically defined surfaces, we produced a novel bacterial identifier termed the surface interaction profile (SIP). The interaction strengths of twelve test bacteria across three discrete polyelectrolyte multilayer films (PEMs) were measured, facilitated by introducing

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Xiaoxia, S., T. Y. Peng, and P. S. Olavi. "Direct force measurement of bacteria adhesion on metal in aqueous media." Water Science and Technology 54, no. 9 (2006): 17–25. http://dx.doi.org/10.2166/wst.2006.866.

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The adhesion of bacteria on metal surfaces in aqueous media and the development of biofilm and resultant biofouling are important phenomena in both the natural environment and engineering systems. This work reports on the use of a force microscopy technique to measure bacterial metal adhesion by two anaerobic sulphate-reducing bacteria (Desulfovibrio desulfuricans and a local marine isolate) and an aerobe (Pseudomonas sp.). Using a modified bacteria tip, the atomic force microscope was able to quantify the attraction and repulsion force in the nano-Newton range between the bacteria cell and me

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Dang, Hongyue, and Charles R. Lovell. "Numerical Dominance and Phylotype Diversity of Marine Rhodobacter Species during Early Colonization of Submerged Surfaces in Coastal Marine Waters as Determined by 16S Ribosomal DNA Sequence Analysis and Fluorescence In Situ Hybridization." Applied and Environmental Microbiology 68, no. 2 (2002): 496–504. http://dx.doi.org/10.1128/aem.68.2.496-504.2002.

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ABSTRACT Early stages of surface colonization in coastal marine waters appear to be dominated by the marine Rhodobacter group of the α subdivision of the division Proteobacteria (α-Proteobacteria). However, the quantitative contribution of this group to primary surface colonization has not been determined. In this study, glass microscope slides were incubated in a salt marsh tidal creek for 3 or 6 days. Colonizing bacteria on the slides were examined by fluorescence in situ hybridization by employing DNA probes targeting 16S or 23S rRNA to identify specific phylogenetic groups. Confocal laser

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Chiang, Chao-Ching, Xinyi Xia, Valentin Craciun, et al. "Enhancing the Hydrophobicity and Antibacterial Properties of SiCN-Coated Surfaces with Quaternization to Address Peri-Implantitis." Materials 16, no. 17 (2023): 5751. http://dx.doi.org/10.3390/ma16175751.

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Peri-implantitis is a major cause of dental implant failure. This disease is an inflammation of the tissues surrounding the implant, and, while the cause is multi-factorial, bacteria is the main culprit in initiating an inflammatory reaction. Dental implants with silicon carbonitride (SiCN) coatings have several potential advantages over traditional titanium implants, but their antibacterial efficiency has not yet been evaluated. The purpose of this study was to determine the anti-bacterial potential of SiCN by modifying the surface of SiCN-coated implants to have a positive charge on the nitr

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Wilson-Nieuwenhuis, Joels, Nina Dempsey-Hibbert, Christopher M. Liauw, and Kathryn A. Whitehead. "The Effect of Human Blood Plasma Conditioning Films on Platelet Transfusion Bag Surface Properties." Applied Sciences 12, no. 22 (2022): 11358. http://dx.doi.org/10.3390/app122211358.

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Transfusion-associated bacterial infections continue to occur which may be due to the formation of bacterial biofilms on the inner surface of the blood bag. Plasticized poly (vinyl chloride) (p-PVC) platelet storage bags in three surface roughness states (rough, smooth and flattened) were used to determine the effect that a conditioning film (CF) of human plasma had on surface properties and its interaction with Staphylococcus epidermidis and Serratia marcescens. SEM and optical profilometry determined changes in surface roughness, whilst EDX and ATR-FTIR determined surface chemistry. The phys

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Wandiyanto, Jason V., Vi Khanh Truong, Mohammad Al Kobaisi, et al. "The Fate of Osteoblast-Like MG-63 Cells on Pre-Infected Bactericidal Nanostructured Titanium Surfaces." Materials 12, no. 10 (2019): 1575. http://dx.doi.org/10.3390/ma12101575.

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Biomaterials that have been newly implanted inside the body are the substratum targets for a “race for the surface”, in which bacterial cells compete against eukaryotic cells for the opportunity to colonize the surface. A victory by the former often results in biomaterial-associated infections, which can be a serious threat to patient health and can undermine the function and performance of the implant. Moreover, bacteria can often have a ‘head start’ if implant contamination has taken place either prior to or during the surgery. Current prevention and treatment strategies often rely on system

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HUI, YEW WOH, and GARY A. DYKES. "Modulation of Cell Surface Hydrophobicity and Attachment of Bacteria to Abiotic Surfaces and Shrimp by Malaysian Herb Extracts." Journal of Food Protection 75, no. 8 (2012): 1507–11. http://dx.doi.org/10.4315/0362-028x.jfp-12-062.

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The use of simple crude water extracts of common herbs to reduce bacterial attachment may be a cost-effective way to control bacterial foodborne pathogens, particularly in developing countries. The ability of water extracts of three common Malaysian herbs (Andrographis paniculata, Eurycoma longifolia, and Garcinia atroviridis) to modulate hydrophobicity and attachment to surfaces of five food-related bacterial strains (Bacillus cereus ATCC 14576, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 10145, Salmonella Enteritidis ATCC 13076, Staphylococcus aureus ATCC 25923) were determined.

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Farkas, Eniko, Robert Tarr, Tamás Gerecsei, et al. "Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing." Biosensors 12, no. 2 (2022): 56. http://dx.doi.org/10.3390/bios12020056.

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Bacteria repellent surfaces and antibody-based coatings for bacterial assays have shown a growing demand in the field of biosensors, and have crucial importance in the design of biomedical devices. However, in-depth investigations and comparisons of possible solutions are still missing. The optical waveguide lightmode spectroscopy (OWLS) technique offers label-free, non-invasive, in situ characterization of protein and bacterial adsorption. Moreover, it has excellent flexibility for testing various surface coatings. Here, we describe an OWLS-based method supporting the development of bacteria

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Droumpali, Ariadni, Jörg Hübner, Lone Gram, and Rafael Taboryski. "Fabrication of Microstructured Surface Topologies for the Promotion of Marine Bacteria Biofilm." Micromachines 12, no. 8 (2021): 926. http://dx.doi.org/10.3390/mi12080926.

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Several marine bacteria of the Roseobacter group can inhibit other microorganisms and are especially antagonistic when growing in biofilms. This aptitude to naturally compete with other bacteria can reduce the need for antibiotics in large-scale aquaculture units, provided that their culture can be promoted and controlled. Micropatterned surfaces may facilitate and promote the biofilm formation of species from the Roseobacter group, due to the increased contact between the cells and the surface material. Our research goal is to fabricate biofilm-optimal micropatterned surfaces and investigate

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Gupta, Akash, Ryan F. Landis, and Vincent M. Rotello. "Nanoparticle-Based Antimicrobials: Surface Functionality is Critical." F1000Research 5 (March 16, 2016): 364. http://dx.doi.org/10.12688/f1000research.7595.1.

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Bacterial infections cause 300 million cases of severe illness each year worldwide. Rapidly accelerating drug resistance further exacerbates this threat to human health. While dispersed (planktonic) bacteria represent a therapeutic challenge, bacterial biofilms present major hurdles for both diagnosis and treatment. Nanoparticles have emerged recently as tools for fighting drug-resistant planktonic bacteria and biofilms. In this review, we present the use of nanoparticles as active antimicrobial agents and drug delivery vehicles for antibacterial therapeutics. We further focus on how surface f

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Yang, Hongying, Wenjie Luo, and Ying Gao. "Effect of Acidithiobacillus ferrooxidans on Humic-Acid Passivation Layer on Pyrite Surface." Minerals 8, no. 10 (2018): 422. http://dx.doi.org/10.3390/min8100422.

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The effect of Acidithiobacillus ferrooxidans on the humic-acid passivation layer on pyrite surfaces was studied by atomic-force microscopy, leaching experiments, and adsorption experiments. Atomic-force-microscopy results showed that humic-acid was adsorbed onto the pyrite surface. The bacteria grew and reproduced on the humic-acid layer. Leaching experiments showed that the humic-acid passivation layer prevented the oxidation of pyrite by Fe3+ under aseptic conditions. Bacteria destroyed the humic-acid layer, promoted pyrite oxidation, and increased the oxidation of pyrite from 1.64% to 67.9%

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Dang, Hongyue, Tiegang Li, Mingna Chen, and Guiqiao Huang. "Cross-Ocean Distribution of Rhodobacterales Bacteria as Primary Surface Colonizers in Temperate Coastal Marine Waters." Applied and Environmental Microbiology 74, no. 1 (2007): 52–60. http://dx.doi.org/10.1128/aem.01400-07.

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ABSTRACT Bacterial surface colonization is a universal adaptation strategy in aquatic environments. However, neither the identities of early colonizers nor the temporal changes in surface assemblages are well understood. To determine the identities of the most common bacterial primary colonizers and to assess the succession process, if any, of the bacterial assemblages during early stages of surface colonization in coastal water of the West Pacific Ocean, nonnutritive inert materials (glass, Plexiglas, and polyvinyl chloride) were employed as test surfaces and incubated in seawater off the Qin

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Venieraki, A., P. Ch Tsalgatidou, D. G. Georgakopoulos, M. Dimou, and P. Katinakis. "Swarming motility in plant-associated bacteria." Hellenic Plant Protection Journal 9, no. 1 (2016): 16–27. http://dx.doi.org/10.1515/hppj-2016-0002.

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Summary Plant-associated environments harbor a huge number of diverse bacteria that compete and/or cooperate for the occupation of the most nutrient-rich ecological niches. Motility, a common trait among bacteria, has long been assumed to provide a survival advantage to skilful bacteria in invading these environments. Bacterial surface motility, such as swarming, a flagella-driven type of surface movement, although mostly observed and studied on agar substrates, is emerging as a major trait involved in many functions of plant-associated bacteria in regard to their ability to colonize and sprea

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Tuson, Hannah H., and Douglas B. Weibel. "Bacteria–surface interactions." Soft Matter 9, no. 17 (2013): 4368. http://dx.doi.org/10.1039/c3sm27705d.

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Zhang, Yirui, Liam Herndon, Punnag Padhy, Baba Ogunlade, Alexandria Boehm, and Jennifer Dionne. "Electrokinetics with Surface-Enhanced Raman Spectroscopy for Bacterial Identification in Wastewater." ECS Meeting Abstracts MA2024-01, no. 33 (2024): 1666. http://dx.doi.org/10.1149/ma2024-01331666mtgabs.

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Bacterial presence in wastewater serves as a crucial bioindicator for monitoring population-level infections and offering early disease outbreak warnings [1,2]. However, identifying bacteria in wastewater presents outstanding challenges; notably, current culturing or fluorescence-based methods [3] to identify bacteria are slow and expensive, unsuitable for high-throughput screening of diverse bacterial species, and may not work well in the complex wastewater matrix. Here, we harness the potential of surface chemistry and electrokinetics to introduce an innovative electro-optical approach that

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Taylor, Gordon T., and Jeanne D. Gulnick. "Enhancement of marine bacterial growth by mineral surfaces." Canadian Journal of Microbiology 42, no. 9 (1996): 911–18. http://dx.doi.org/10.1139/m96-117.

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The effects of sorptive inert surfaces on growth of marine bacteria and metabolism, as well as partitioning of organic substrates, were examined in microcosms inoculated with bacterioplankton from a local salt marsh. Introduction of organic-free glass beads to a dilute seawater medium (tryptic soy broth) increased yields of ATP, a surrogate for bacterial biomass, by 187% within the entire microcosm (attached + free-living). Growth efficiencies (bacterial C/media C) were 30% for bacteria grown in microcosms with beads compared with 16% without beads. Surface enrichment increased rates of proteo

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Nielsen, J. L., L. H. Mikkelsen, and P. H. Nielsen. "In situ detection of cell surface hydrophobicity of probe-defined bacteria in activated sludge." Water Science and Technology 43, no. 6 (2001): 97–103. http://dx.doi.org/10.2166/wst.2001.0349.

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The surface hydrophobicity of different types of bacteria in activated sludge were investigated under in situ conditions by following the adhesion of fluorescent microspheres with defined surface properties to bacterial surfaces (the MAC-method). This technique was combined with identification of the bacteria with fluorescence in situ hybridization with rRNA-targeted oligonucleotides (FISH) and could thus be used for characterization of surface properties of probe-defined bacteria directly in a complex system without prior enrichment or isolation. This MAC-FISH technique could be used for sing

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Gahlot, Dharmender K., Nayyer Taheri, and Sheila MacIntyre. "Diversity in Genetic Regulation of Bacterial Fimbriae Assembled by the Chaperone Usher Pathway." International Journal of Molecular Sciences 24, no. 1 (2022): 161. http://dx.doi.org/10.3390/ijms24010161.

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Bacteria express different types of hair-like proteinaceous appendages on their cell surface known as pili or fimbriae. These filamentous structures are primarily involved in the adherence of bacteria to both abiotic and biotic surfaces for biofilm formation and/or virulence of non-pathogenic and pathogenic bacteria. In pathogenic bacteria, especially Gram-negative bacteria, fimbriae play a key role in bacteria–host interactions which are critical for bacterial invasion and infection. Fimbriae assembled by the Chaperone Usher pathway (CUP) are widespread within the Enterobacteriaceae, and thei

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Matz, Carsten, and Klaus Jürgens. "Effects of Hydrophobic and Electrostatic Cell Surface Properties of Bacteria on Feeding Rates of Heterotrophic Nanoflagellates." Applied and Environmental Microbiology 67, no. 2 (2001): 814–20. http://dx.doi.org/10.1128/aem.67.2.814-820.2001.

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ABSTRACT The influence of cell surface hydrophobicity and electrostatic charge of bacteria on grazing rates of three common species of interception-feeding nanoflagellates was examined. The hydrophobicity of bacteria isolated from freshwater plankton was assessed by using two different methods (bacterial adhesion to hydrocarbon and hydrophobic interaction chromatography). The electrostatic charge of the cell surface (measured as zeta potential) was analyzed by microelectrophoresis. Bacterial ingestion rates were determined by enumerating bacteria in food vacuoles by immunofluorescence labellin

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Vymazal, J., J. Balcarová, and H. Doušová. "Bacterial dynamics in the sub-surface constructed wetland." Water Science and Technology 44, no. 11-12 (2001): 207–9. http://dx.doi.org/10.2166/wst.2001.0830.

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Constructed wetlands have been shown to be capable of removing a wide variety of contaminants, including bacterial pollution. However, only limited information exists on the distribution of bacteria on roots of macrophytes growing in constructed wetlands. Constructed wetland with sub-surface horizontal flow at Nucÿice near Prague, Czech Republic, was put in operation in 1996. The system treats municipal sewage from 650 PE and the total area of the beds, planted with Phalaris arundinacea and Phragmites australis in alternate stripes perpendicular to the flow direction, is 3,224 m2 (2 beds 62×26

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Sejati, Bramasto Purbo, Tetiana Haniastuti, Ahmad Kusumaatmaja, and Maria Goreti Widyastuti. "The Influence of Surface Damage on Miniplates: A Study of Bacterial Attachment Across Various Strains." F1000Research 14 (February 4, 2025): 158. https://doi.org/10.12688/f1000research.159954.1.

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Background Miniplates are frequently used in oral and maxillofacial surgery to address malocclusion issues. However, surface damage to miniplates is a significant concern that can affect surgical outcomes and patient quality of life. This study aims to evaluate the influence of miniplate surface damage on bacterial attachment, which may lead to postoperative infections. Methods Miniplates with varying degrees of surface damage were used in this study. The damaged surfaces were subjected to special treatments to simulate postoperative conditions. Various bacterial strains, including Staphylococ

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Zhurlov, Oleg, and Maria Zhurlova. "The effectiveness of bacterial contamination of beetles during their transfer to wheat bran." BIO Web of Conferences 130 (2024): 07004. http://dx.doi.org/10.1051/bioconf/202413007004.

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The study of the mechanisms of transfer of bacteria during contamination of insect surfaces during food invasion is of current interest. Additionally, the increasing antibiotic resistance of clinical bacterial strains over the past decade has highlighted the importance of understanding the mechanisms of bacterial circulation among insects, especially antibiotic-resistant strains. Our modeling study demonstrates that the transfer of the Escherichia coli Rif (+) marker strain on the surface and inside the intestine of adult beetles Tenebrio molitor contributes to bacterial contamination of wheat

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Müller, Rainer, Gerhard Gröger, Karl-Anton Hiller, Gottfried Schmalz, and Stefan Ruhl. "Fluorescence-Based Bacterial Overlay Method for Simultaneous In Situ Quantification of Surface-Attached Bacteria." Applied and Environmental Microbiology 73, no. 8 (2007): 2653–60. http://dx.doi.org/10.1128/aem.02884-06.

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ABSTRACT For quantification of bacterial adherence to biomaterial surfaces or to other surfaces prone to biofouling, there is a need for methods that allow a comparative analysis of small material specimens. A new method for quantification of surface-attached biotinylated bacteria was established by in situ detection with fluorescence-labeled avidin-D. This method was evaluated utilizing a silicon wafer model system to monitor the influences of surface wettability and roughness on bacterial adhesion. Furthermore, the effects of protein preadsorption from serum, saliva, human serum albumin, and

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Brás, Ana, Márcia Braz, Inês Martinho, João Duarte, Carla Pereira, and Adelaide Almeida. "Effect of Bacteriophages against Biofilms of Escherichia coli on Food Processing Surfaces." Microorganisms 12, no. 2 (2024): 366. http://dx.doi.org/10.3390/microorganisms12020366.

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The bacterial adhesion to food processing surfaces is a threat to human health, as these surfaces can serve as reservoirs of pathogenic bacteria. Escherichia coli is an easily biofilm-forming bacterium involved in surface contamination that can lead to the cross-contamination of food. Despite the application of disinfection protocols, contamination through food processing surfaces continues to occur. Hence, new, effective, and sustainable alternative approaches are needed. Bacteriophages (or simply phages), viruses that only infect bacteria, have proven to be effective in reducing biofilms. He

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Karlsson, Christofer, Matthias Mörgelin, Mattias Collin, et al. "SufA – a bacterial enzyme that cleaves fibrinogen and blocks fibrin network formation." Microbiology 155, no. 1 (2009): 238–48. http://dx.doi.org/10.1099/mic.0.021311-0.

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Finegoldia magna is a member of the normal human bacterial flora on the skin and other non-sterile body surfaces, but this anaerobic coccus is also an important opportunistic pathogen. SufA was the first F. magna proteinase to be isolated and characterized. Many bacterial pathogens interfere with different steps of blood coagulation, and here we describe how purified SufA efficiently and specifically cleaves fibrinogen in human plasma. SufA is both secreted by F. magna and associated with the bacterial surface. Successful gene targeting has previously not been performed in anaerobic cocci, but

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Oliveira, Nuno M., Kevin R. Foster, and William M. Durham. "Single-cell twitching chemotaxis in developing biofilms." Proceedings of the National Academy of Sciences 113, no. 23 (2016): 6532–37. http://dx.doi.org/10.1073/pnas.1600760113.

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Bacteria form surface-attached communities, known as biofilms, which are central to bacterial biology and how they affect us. Although surface-attached bacteria often experience strong chemical gradients, it remains unclear whether single cells can effectively perform chemotaxis on surfaces. Here we use microfluidic chemical gradients and massively parallel automated tracking to study the behavior of the pathogenPseudomonas aeruginosaduring early biofilm development. We show that individual cells can efficiently move toward chemoattractants using pili-based “twitching” motility and the Chp che

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de Avila, Erica Dorigatti, Rafael Scaf de Molon, Bruno P. Lima, et al. "Impact of Physical Chemical Characteristics of Abutment Implant Surfaces on Bacteria Adhesion." Journal of Oral Implantology 42, no. 2 (2016): 153–58. http://dx.doi.org/10.1563/aaid-joi-d-14-00318.

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Surface attachment is the first step in biofilm formation, and the ability of bacteria to adhere to surfaces and develop a biofilm is directly influenced by electrostatic interactions between the bacteria and the chemical composition of material surfaces. Here, we investigated the influence of physical and chemical characteristics of titanium (Ti) and zirconia (ZrO2) as implant abutment surfaces on the bacterial adhesion phase and compared the results to bovine enamel (BE) simulating a human tooth. To achieve this goal, we used 2 common pathogens of the oral cavity, Streptococcus mutans UA140

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MAI, TAM L., NOFRIJON I. SOFYAN, JEFFREY W. FERGUS, WILLIAM F. GALE, and DONALD E. CONNER. "Attachment of Listeria monocytogenes to an Austenitic Stainless Steel after Welding and Accelerated Corrosion Treatments." Journal of Food Protection 69, no. 7 (2006): 1527–32. http://dx.doi.org/10.4315/0362-028x-69.7.1527.

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Austenitic stainless steels, widely used in food processing, undergo microstructural changes during welding, resulting in three distinctive zones: weld metal, heat-affected zone, and base metal. This research was conducted to determine the attachment of Listeria monocytogenes in these three zones before and after exposure to a corrosive environment. All experiments were done with tungsten inert gas welding of type 304 stainless steel. The four welding treatments were large or small beads with high or low heat. After welding, all surfaces were polished to an equivalent surface finish. A 10-μl d

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Stenfors, Lars-Eric, Helga-Marie Bye, and Simo Räisänen. "Immunocytochemical localization of lysozyme and lactoferrin attached to surface bacteria of the palatine tonsils during infectious mononucleosis." Journal of Laryngology & Otology 116, no. 4 (2002): 264–68. http://dx.doi.org/10.1258/0022215021910717.

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Bacterial samples were obtained from the tonsillar surfaces of seven patients (four males, three females; median age 18 years, range 15 to 21 years) suffering from acute infectious mononucleosis with concomitant pharyngotonsillitis, and from five healthy controls. By using gold-labelled antiserum to human lysozyme and lactoferrin, micro-organisms on the tonsillar surfaces coated with these antibacterial substances could be identified by tracing the gold particles in the transmission electron microscope. In healthy individuals, most of the bacteria were coated with lysozyme and significantly mo

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Bennett, Rachel R., Calvin K. Lee, Jaime De Anda, et al. "Species-dependent hydrodynamics of flagellum-tethered bacteria in early biofilm development." Journal of The Royal Society Interface 13, no. 115 (2016): 20150966. http://dx.doi.org/10.1098/rsif.2015.0966.

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Monotrichous bacteria on surfaces exhibit complex spinning movements. Such spinning motility is often a part of the surface detachment launch sequence of these cells. To understand the impact of spinning motility on bacterial surface interactions, we develop a hydrodynamic model of a surface-bound bacterium, which reproduces behaviours that we observe in Pseudomonas aeruginosa , Shewanella oneidensis and Vibrio cholerae , and provides a detailed dictionary for connecting observed spinning behaviour to bacteria–surface interactions. Our findings indicate that the fraction of the flagellar filam

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Corcionivoschi, Nicolae, Igori Balta, Eugenia Butucel, et al. "Natural Antimicrobial Mixtures Disrupt Attachment and Survival of E. coli and C. jejuni to Non-Organic and Organic Surfaces." Foods 12, no. 20 (2023): 3863. http://dx.doi.org/10.3390/foods12203863.

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The contact and adherence of bacteria to various surfaces has significant consequences on biofilm formation through changes in bacterial surface structures or gene expression with potential ramifications on plant and animal health. Therefore, this study aimed to investigate the effect of organic acid-based mixtures (Ac) on the ability Campylobacter jejuni and Escherichia coli to attach and form biofilm on various surfaces, including plastic, chicken carcass skins, straw bedding, and eggshells. Moreover, we aimed to explore the effect of Ac on the expression of E. coli (luxS, fimC, csgD) and C.

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Ferreira, Sílvia, and Ana P. Piedade. "Influence of Extracellular Mimicked Hierarchical Nano-Micro-Topography on the Bacteria/Abiotic Interface." Polymers 12, no. 4 (2020): 828. http://dx.doi.org/10.3390/polym12040828.

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The study of interfaces between engineered surfaces and prokaryotic cells is a subject whose actual relevance has been reinforced by the current outbreaks due to unknown viruses and antibiotic-resistant bacteria. Studies aiming at the development of antibacterial surfaces are based on two pillars: surface chemistry or topographical cues. This work reports the study of only the topographic aspect by the development of thin films of polyamide, which present attractive surface chemistry for bacterial adhesion. The same chemistry with only nano- or hierarchical nano- and micro-topography that mimi

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Santo, Christophe Espírito, Nadine Taudte, Dietrich H. Nies, and Gregor Grass. "Contribution of Copper Ion Resistance to Survival of Escherichia coli on Metallic Copper Surfaces." Applied and Environmental Microbiology 74, no. 4 (2007): 977–86. http://dx.doi.org/10.1128/aem.01938-07.

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ABSTRACT Bacterial contamination of touch surfaces poses a serious threat for public health. The use of bactericidal surface materials, such as copper and its alloys, might constitute a way to aid the use of antibiotics and disinfectants, thus minimizing the risk of emergence and spread of multiresistant germs. The survival of Escherichia coli on metallic copper surfaces has been studied previously; however, the mechanisms underlying bacterial inactivation on copper surfaces have not been elucidated. Data presented in this study suggest that bacteria are killed rapidly on dry copper surfaces.

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Galachyants, Agnia, Irina Tomberg, Elena Sukhanova, et al. "Bacterioneuston in Lake Baikal: Abundance, Spatial and Temporal Distribution." International Journal of Environmental Research and Public Health 15, no. 11 (2018): 2587. http://dx.doi.org/10.3390/ijerph15112587.

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An aquatic surface microlayer covers more than 70% of the world’s surface. Our knowledge about the biology of the surface microlayer of Lake Baikal, the most ancient lake on Earth with a surface area of 31,500 km2, is still scarce. The total bacterial abundance, the number of cultured heterotrophic temporal bacteria, and the spatial distribution of bacteria in the surface microlayer and underlying waters of Lake Baikal were studied. For the first time, the chemical composition of the surface microlayer of Lake Baikal was determined. There were significant differences and a direct relationship

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Mathews, Salima, Michael Hans, Frank Mücklich, and Marc Solioz. "Contact Killing of Bacteria on Copper Is Suppressed if Bacterial-Metal Contact Is Prevented and Is Induced on Iron by Copper Ions." Applied and Environmental Microbiology 79, no. 8 (2013): 2605–11. http://dx.doi.org/10.1128/aem.03608-12.

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ABSTRACTBacteria are rapidly killed on copper surfaces, and copper ions released from the surface have been proposed to play a major role in the killing process. However, it has remained unclear whether contact of the bacteria with the copper surface is also an important factor. Using laser interference lithography, we engineered copper surfaces which were covered with a grid of an inert polymer which prevented contact of the bacteria with the surface. UsingEnterococcus hiraeas a model organism, we showed that the release of ionic copper from these modified surfaces was not significantly reduc

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Hemmatian and Kim. "Quantification Methods for Textile-Adhered Bacteria: Extraction, Colorimetric, and Microscopic Analysis." Polymers 11, no. 10 (2019): 1666. http://dx.doi.org/10.3390/polym11101666.

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Quantification of bacteria adhered on porous, multi-layered fibers is a challenging task. The goal of this study is to compare different assessment procedures on counting textile-adhered bacteria, and to guide relevant analytical techniques. Three different methods were compared in measuring the amount of Escherichia coli (E. coli) adhered to polymeric film and fibrous nonwovens. In the extraction method, the adhered bacteria were released with the assistance of surfactant/enzyme, where the measurement was rather reproducible. For colorimetric method, stained bacteria enabled direct visualizat

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van Doorn, W. G., and Y. de Witte. "Sources of the Bacteria Involved in Vascular Occlusion of Cut Rose Flowers." Journal of the American Society for Horticultural Science 122, no. 2 (1997): 263–66. http://dx.doi.org/10.21273/jashs.122.2.263.

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Cut rose (Rosa hybrida L.) flowers placed in water often wilt prematurely, which is partially due to bacterial accumulation in the stems. Bacterial strains in the stems are mainly pseudomonads and enterobacteria. The possible sources of these organisms were investigated in `Sweet Promise' (trade name Sonia) roses. No bacteria were found in the xylem of intact plants. Cutting the stems with sterile secateurs introduced no bacteria at the cut surface or the stem interior, but cutting with nonsterile secateurs used by rose growers did. The secateurs sampled at rose growers contained Enterobacter

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Udijono, Ari, Agus Subagio, Mateus Sakundarno Adi, et al. "Students’ Habits and Identification of Bacteria on Inanimate Surface of Educational Setting." JURNAL KESEHATAN LINGKUNGAN 16, no. 4 (2024): 360–67. http://dx.doi.org/10.20473/jkl.v16i4.2024.360-367.

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Introduction: Diponegoro Clinic reported 29.1% of students seek infectious diseases treatment. Student habits in the classroom were thought to play roles in the presence of bacteria. Surfaces of inanimate objects in the classroom were potential source for bacteria. The research objectives were analyzing student’s habits and identifying bacteria on the surface of inanimate objects in the classroom. Methods: Four types of samples, including the surfaces of tables, chairs, flips of air conditioners, and floors were taken from 13 faculties at Universitas Diponegoro. Plate count agar media were use

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