Academic literature on the topic 'Survival. eng'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Survival. eng.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "Survival. eng"
1
Cirlot, Victoria. "The pathos formulae and their survival (ENG/ESP)." Comparative Cinema 7, no. 12 (May 18, 2019): 7–21. http://dx.doi.org/10.31009/cc.2019.v7.i12.01.
Full textAbstract:
This article attempts to set out and understand what art historian Aby Warburg called Pathosformel (pathos formulae), a key concept in his studies, though he never expressly offered a definition, nor explained what his understanding of it was. The emergence of the concept is traced in his work, deciphering its meaning and its implications for the analysis of images, also from the most significant and recent bibliography on the subject. Finally, the Pathosformel concept is applied to a contemporary photographic image which enables us to better understand its effect in the way it is received by its viewers, thus establishing a dialogue between the iconographic tradition of the past and the present.
2
Bruno, R., J. Lu, Y. Sun, and L. Claret. "Simulation of survival with first- and second-line non-small cell lung cancer (NSCLC) therapy using a public domain drug-disease modeling framework." Journal of Clinical Oncology 27, no. 15_suppl (May 20, 2009): 8087. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.8087.
Full textAbstract:
8087 Background: Modeling and simulation approaches are advocated to support drug development decisions. In this study, we evaluated the ability of a drug-disease model recently developed by scientists at the FDA using data from pivotal studies in NSCLC (Wang et al. http://www.fda.gov/ohrms/dockets/ac/08/briefing/2008–4351b1–00-index.htm ) to simulate survival and tumor response for approved therapies in NSCLC. Methods: The modeling framework comprises a longitudinal tumor size model and a survival model relating change in tumor size at first visit (week 8) and patient characteristics (tumor size and ECOG performance status at baseline) to survival time. The tumor size and survival models were used to simulate change in tumor size at first visit and expected survival in the carboplatin/paclitaxel (CP) arm of the E4599 study (first-line NSCLC, N Eng J Med. 2006;355:2542–2550) and in the erlotinib arm in the BR.21 study (second-line NSCLC, N Eng J Med. 2005;353:123–132). The predictive distributions (95% prediction interval [PI]) of survival times were derived from multiple replicates (500) of 400 CP patients and 500 erlotinib patients with similar characteristics to patients in the original studies. Results: There was a high level of concordance between the results of the simulation and the observed results in the two arms, indicating that the modeling framework successfully predicted survival and tumor response. Expected median survival was 9.8 (95% PI, 8.2–11.8) months (vs 10.3 months observed) for the CP arm and 6.8 (95% PI, 5.3–8.9) months (vs 6.7 months observed) for the erlotinib arm. The median change in relative tumor size from baseline at week 8 predicted by the model was 12.1% for the CP arm and 9.5% for the erlotinib arm. Conclusions: The modeling framework can be used to perform simulations of survival of approved treatments. These results suggest that the model could be used to simulate survival for investigational treatments based on tumor shrinkage data observed in early clinical studies (e.g. phase II) to support end-of-phase II decisions and the design of phase III studies. [Table: see text]
3
Wang, Xinyu, Jiaojiao Yang, and Xueren Gao. "Identification of key genes associated with lung adenocarcinoma by bioinformatics analysis." Science Progress 104, no. 1 (January 2021): 003685042199727. http://dx.doi.org/10.1177/0036850421997276.
Full textAbstract:
Lung adenocarcinoma (LUAD) is the most common histological type of lung cancer, comprising around 40% of all lung cancer. Until now, the pathogenesis of LUAD has not been fully elucidated. In the current study, we comprehensively analyzed the dysregulated genes in lung adenocarcinoma by mining public datasets. Two sets of gene expression datasets were obtained from the Gene Expression Omnibus (GEO) database. The dysregulated genes were identified by using the GEO2R online tool, and analyzed by R packages, Cytoscape software, STRING, and GPEIA online tools. A total of 275 common dysregulated genes were identified in two independent datasets, including 54 common up-regulated and 221 common down-regulated genes in LUAD. Gene Ontology (GO) enrichment analysis showed that these dysregulated genes were significantly enriched in 258 biological processes (BPs), 27 cellular components (CCs), and 21 molecular functions (MFs). Furthermore, protein-protein interaction (PPI) network analysis showed that PECAM1, ENG, KLF4, CDH5, and VWF were key genes. Survival analysis indicated that the low expression of ENG was associated with poor overall survival (OS) of LUAD patients. The low expression of PECAM1 was associated with poor OS and recurrence-free survival of LUAD patients. The cox regression model developed based on age, tumor stage, ENG, PECAM1 could effectively predict 5-year survival of LUAD patients. This study revealed some key genes, BPs, CCs, and MFs involved in LUAD, which would provide new insights into understanding the pathogenesis of LUAD. In addition, ENG and PECAM1 might serve as promising prognostic markers in LUAD.
4
Mu-Mosley, Hong, Lauren B. Ostermann, Ran Zhao, Challice L. Bonifant, Stephen Gottschalk, Mireya Paulina Velasquez, and Michael Andreeff. "Venetoclax Enhances Anti-Leukemia Activity of CD123-Specific BiTE-Secreting T-Cells in AML." Blood 136, Supplement 1 (November 5, 2020): 12–13. http://dx.doi.org/10.1182/blood-2020-139625.
Full textAbstract:
Background: CD123 is frequently expressed in hematologic malignancies including AML. CD123 has been a potential immunotherapeutic target in AML due to its association with leukemic stem cells that play an essential role in disease progression and relapse. Our previous study using T-cells secreting CD123/CD3-bispecific T-cell engagers (BiTEs) (CD123-ENG T-cells) has shown activity in preclinical studies, recognizing and killing acute myeloid leukemia (AML) blasts in vitro and in vivo. CD123-ENG T-cells secrete bispecific molecules that recognize CD3 (T-cells) and CD123 (AML blasts), and are able to direct transduced T-cells and recruit bystander T-cells to kill CD123-positive blasts. Venetoclax is a BCL-2 inhibitor that can restore functional apoptosis signaling in AML cells, and has been FDA approved for the treatment of AML patients in combination with hypomethylating agents. To improve the efficacy of CD123-ENG T-cells we explored efficacy in AML by combining targeted immunotherapy (CD123-ENG T cells) with targeted inhibition of anti-apoptotic BCL-2 (venetoclax) in vitro and in vivo models of AML. Methods : CD123-ENG T-cells were generated by retroviral transduction and in vitro expansion. Non-transduced (NT) T-cells served as control. In vitro, GFP+ MOLM-13 AML cells were pretreated with venetoclax (0, 10µM, and 20µM) for 24 hours prior to co-culture with CD123-ENG or NT T-cells at an effector/target ratio of 1:10. After 16 hours, MOLM-13 AML cells were analyzed by flow cytometry and quantitated using counting beads; cytotoxicity was calculated relative to untreated MOLM-13 control. The anti-AML activity of the combination was further evaluated in a MOLM-13-luciferase xenograft AML mouse model. Leukemia progression was assessed by bioluminescence imaging. The frequency of MOLM13 AML and human T cells in periphera blod (PB) was determined by flow cytometry. Results: In vitro, we demonstrated that pretreatment of Molm13 AML cells with venetoclax enhanced the cytolytic activity of CD123-ENG T-cells compared to NT- or no T-cell controls. Interestingly, venetoclax sensitized Molm13 to CD123-ENG T-cell killing in a dose-dependent manner (Fig.1; 50%/31% killing by CD123-ENG T-cells versus 27%/14% of killing by NT T cells post pretreatment with 10µM or 20µM ventoclax, p<0.001). In the Molm13 luciferase xenograft model, NSGS mice were randomized into 5 groups after AML engraftment was confirmed: 1) vehicle control, 2) Venetoclax (Ven) only, 3) CD123-ENG T-cells only, 4) Ven+CD123-ENG T-cells, 5) Ven+CD123-ENG T-cells/2-day-off Ven post T-cell infusion (Ven[2-day-off]+CD123-ENG). Venetoclax treatment (100 µg/kg daily via oral gavage) was started on day 4 post Molm13 injection, and on day 7, mice received one i.v. dose of CD123-ENG T-cells (5x106 cells/mouse). Venetoclax or CD123-ENG T-cell monotherapy reduced leukemia burden compared to the control group, and combinational treatments further inhibited leukemia progression as judged by BLI and circulating AML cells (%GFP+mCD45-/total live cells) by flow cytometry on day 15 post MOLM-13 injection: vehicle control: 19.6%; Ven+: 3.4%; CD123-ENG T-cells:1.2 %; Ven+CD123-ENG T-cells: 0.3%; Ven[2-day-off]+CD123-ENG T-cells (p<0.01 Ven+ or CD123-ENG T-cells versus control; p<0.001 Ven+CD123-ENG or Ven[2-day-off]+CD123-ENG T cells versus CD123-ENG T cells, n=5). The enhanced anti-AML activity of combining venetoclax and CD123-ENG T-cells translated into a significant survival benefit in comparison to single treatment alone (Fig. 2). However, while Ven+CD123-ENG and Ven[2-day-off]+CD123-ENG T-cell treated mice had a survival advantage, they had reduced circulating numbers of human CD3+ T cells on day 8 post T-cells infusion compared to mice that received CD123-ENG T-cells, indicative of potential adverse effect of venetoclax on T-cell survival in vivo. Conclusion: Our data support a concept of combining pro-apoptotic targeted and immune therapy using venetoclax and CD123-ENG T-cells in AML. While it has been reported that venetoclax does not impair T-cell functionality, more in-depth analysis of the effect of Bcl-2 inhibition on T-cell function and survival appears warranted, as it could diminish survival not only of AML blasts but also of immune cells. Disclosures Bonifant: Patents filed in the field of engineered cellular therapies: Patents & Royalties: Patents filed in the field of engineered cellular therapies. Gottschalk:Patents and patent applications in the fields of T-cell & Gene therapy for cancer: Patents & Royalties; Inmatics and Tidal: Membership on an entity's Board of Directors or advisory committees; Merck and ViraCyte: Consultancy; TESSA Therapeutics: Other: research collaboration. Velasquez:Rally! Foundation: Membership on an entity's Board of Directors or advisory committees; St. Jude: Patents & Royalties. Andreeff:Amgen: Research Funding; Daiichi-Sankyo; Jazz Pharmaceuticals; Celgene; Amgen; AstraZeneca; 6 Dimensions Capital: Consultancy; Daiichi-Sankyo; Breast Cancer Research Foundation; CPRIT; NIH/NCI; Amgen; AstraZeneca: Research Funding; Centre for Drug Research & Development; Cancer UK; NCI-CTEP; German Research Council; Leukemia Lymphoma Foundation (LLS); NCI-RDCRN (Rare Disease Clin Network); CLL Founcdation; BioLineRx; SentiBio; Aptose Biosciences, Inc: Membership on an entity's Board of Directors or advisory committees.
5
Mitry, E., A. Fields, H. Bleiberg, R. Labianca, G. Portier, D. Tu, V. Torri, et al. "Adjuvant chemotherapy after potentially curative resection of metastases from colorectal cancer. A meta-analysis of two randomized trials." Journal of Clinical Oncology 24, no. 18_suppl (June 20, 2006): 3524. http://dx.doi.org/10.1200/jco.2006.24.18_suppl.3524.
Full textAbstract:
3524 Background: Adjuvant systemic chemotherapy (CT) administered after resection of colorectal cancer (CRC) metastases (M) may reduce the risk of recurrence and improved survival but its benefit has never been demonstrated. Two phase III trials (FFCD 9002 and EORTC/NCIC CTG/GIVIO (ENG) trials) with a very similar design showed a trend for improvement in survival after adjuvant CT but had to close prematurely because of slow accrual, lacking the statistical power to demonstrate any significant difference in survival. We report here a pooled analysis based on individual data from these trials. Methods: Patients were required to have a WHO performance status ≤2 and a histologically proven CRC with a complete (R0) surgical resection of the primary tumour and of ≤4 liver or lung metastases. They were randomized between chemotherapy (CT arm) [5FU 400 mg/m2 (FFCD) or 370 mg/m2 (ENG) IV q.d. x 5 days plus dl-leucovorin 200 mg/m2 (FFCD) or l-leucovorin 100 mg/m2 (ENG) IV q.d. x 5 days, 6 cycles at 28 days intervals] or surgery alone (S arm). Results: 129 pts were included in the ENG trial between 1994 and 1998, 173 in the FFCD trial between 1991 and 2001. 24 pts (ENG: 22, FFCD: 2) were excluded from analysis for missing post-baseline data: 278 pts were included in the present analysis (CT: 138, S: 140). Patients’ characteristics by treatment arm (% CT/S): males 58.0/63.6, age <70 years: 79.7/79.3, stage IV primary tumor: 29.0/47.1 (p=0.02), liver M 94.2/93.6, ≥2 M resected: 33.3/31.4. Conclusion: Adjuvant CT with a 5FU bolus based regimen tends to improve survival after complete resection of CRC metastases. The observed improvement in median PFS was almost statistically significant whereas the improvement in median OS (more than 1 year) was not (lack of statistical power?). This pooled analysis supports the use of adjuvant CT, with a more effective regimen, after potentially curative resection of CRC metastases. Updated results will be presented. (Supported by AROLD) [Table: see text] No significant financial relationships to disclose.
6
Li, Shan, Yan Zhang, Aishe Gao, Yue Zhang, and Jiong Zhang. "Engelharquinone suppresses lipopolysaccharide-induced inflammation and proliferation of human liver cancer SMCC7721 cells via inhibition of NF-κB and MAPK signaling pathway." Tropical Journal of Pharmaceutical Research 19, no. 4 (May 14, 2020): 699–706. http://dx.doi.org/10.4314/tjpr.v19i4.4.
Full textAbstract:
Purpose: To investigate the anti-tumor effect of engelharquinone (Eng) on human liver cancer SMCC7721 cells.Methods: GFP-labeled SMCC7721 cells were used to establish a tumor-bearing mice model used for determination of the effect of different concentrations of Eng on tumor growth. The effect of Eng on SMCC7721 cell viability was determined with MTT assay and cell cycle analysis. The anti-inflammatory effect of Eng on lipopolysaccharide (LPS)-treated SMCC7721 cells were determined through assay of pro-inflammatory cytokines. Besides, the effect of Eng on the expressions of mitogen-activated protein kinase (MAPK), toll-like receptor 4 (TLR4), and nuclear factor kappa B (NF-κB) was determined.Results: Cell proliferation was suppressed by different concentrations of Eng in LPS-treated SMCC7721 cells. Treatment of nude mice with Eng at high and low doses resulted in significant suppression of tumor growth and marked increases in percentage survival. Treatment of SMCC7721 cells with LPS upregulated the expressions of NF-κB, p65 and MAPK. However, pre-treatment of the cells with Eng suppressed the LPS-induced upregulation of the NF-κB, p65 and MAPK signaling pathways, and further downregulated the production of inflammatory cytokines in SMCC7721 cells.Conclusion: These results indicate that Eng inhibits LPS-induced inflammation and proliferation of human liver cancer SMCC7721 cells via a mechanism involving regulation of NF-κB and MAPK signaling pathways. Thus, Eng has potentials for the clinical management of inflammatory diseases and liver cancer.
Keywords: Inflammation, Engelharquinone, Lipopolysaccharide, SMCC7721 cells, Toll-like receptor 4
7
Mitry, Emmanuel, Anthony L. A. Fields, Harry Bleiberg, Roberto Labianca, Guillaume Portier, Dongsheng Tu, Donato Nitti, et al. "Adjuvant Chemotherapy After Potentially Curative Resection of Metastases From Colorectal Cancer: A Pooled Analysis of Two Randomized Trials." Journal of Clinical Oncology 26, no. 30 (October 20, 2008): 4906–11. http://dx.doi.org/10.1200/jco.2008.17.3781.
Full textAbstract:
Purpose Adjuvant systemic chemotherapy administered after surgical resection of colorectal cancer metastases may reduce the risk of recurrence and improve survival, but its benefit has never been demonstrated. Two phase III trials (Fédération Francophone de Cancérologie Digestive [FFCD] Trial 9002 and the European Organisation for Research and Treatment of Cancer/National Cancer Institute of Canada Clinical Trials Group/Gruppo Italiano di Valutazione Interventi in Oncologia [ENG] trial) used a similar design and showed a trend favoring adjuvant chemotherapy, but both had to close prematurely because of slow accrual, thus lacking the statistical power to demonstrate the predefined difference in survival. We report here a pooled analysis based on individual data from these two trials. Patients and Methods After complete resection of colorectal liver or lung metastases, patients were randomly assigned to chemotherapy (CT arm; fluorouracil [FU] 400 mg/m2 administered intravenously [IV] once daily plus dl-leucovorin 200 mg/m2 [FFCD] × 5 days or FU 370 mg/m2 plus l-leucovorin 100 mg/m2 IV × 5 days [ENG] for six cycles at 28-day intervals) or to surgery alone (S arm). Results A total of 278 patients (CT, n = 138; S, n = 140) were included in the pooled analysis. Median progression-free survival was 27.9 months in the CT arm as compared with 18.8 months in the S arm (hazard ratio = 1.32; 95% CI, 1.00 to 1.76; P = .058). Median overall survival was 62.2 months in the CT arm compared with 47.3 months in the S arm (hazard ratio = 1.32; 95% CI, 0.95 to 1.82; P = .095). Adjuvant chemotherapy was independently associated with both progression-free survival and overall survival in multivariable analysis. Conclusion This pooled analysis shows a marginal statistical significance in favor of adjuvant chemotherapy with an FU bolus–based regimen after complete resection of colorectal cancer metastases.
8
Salmukhanbetova, Zhuldyz, A. A. Imanalinova, L. A. Dimeyeva, and N. E. Zverev. "State of Saxaul Plantations in Kazakhstan’s Section of the Aral Sea Region." Central Asian Journal of Water Research 7, no. 1 (June 30, 2020): 128–57. http://dx.doi.org/10.29258/cajwr/2020-r1.v7-1/128-157.eng.
Full textAbstract:
The article aims to assess the survival rate of saxaul plantations on the dry seabed of the Aral Sea (DSAS) and these close to the villages along the original seacoast. Protective black saxaul (Haloxylon ammodendron) plantations on the DSAS were established in the course of 2009-2019 with the grant support of the International Fund for Saving the Aral Sea (IFAS), Japan’s environmental funds, UNDP Kazakhstan, as well as under the Grass-Roots Program of the Embassy of Japan in Kazakhstan. During September 1-14, 2020, the target plantation plots at 24 sites adjacent to the villages of Aralkum and Karateren underwent examination, including forest surveying, projected species cover determination, as well as seed regeneration and survival rate assessment. The findings of 2020 revealed the varying condition of target saxaul plantations. Thus, the survival rate of saxaul inside forest plantations ranged between 0.12 and 78.0%. The actual number of saxaul trees varied from 1 (one) to 1,000 per ha on the DSAS and from 888 to 2,633 per ha in Aralkum village. The novelty of the obtained results is due to the fact that they clearly demonstrate that the survival rate and development of forest plantations, as well as saxaul seed self-renewal, above all, depend on the overall ecological conditions at specific sites. Saxaul demonstrated the best survival rate and growth at the sites with sandy loam and saline light loamy soils with sandy cover, and the worst – at the sites with crusty and takyr (dry-type playa) saline soils.
9
Velasquez, Mireya Paulina, Kota Iwahori, David L. Torres, Sunitha Kakarla, Caroline Arber, Tania Rodriguez-Cruz, Claudia Gerken, Xiao-Tong Song, and Stephen Gottschalk. "T Cells Expressing Engager and Costimulatory Molecules for the Immunotherapy of CD19+ Malignancies." Blood 124, no. 21 (December 6, 2014): 2433. http://dx.doi.org/10.1182/blood.v124.21.2433.2433.
Full textAbstract:
Abstract Background: Immunotherapy with anti-CD19/anti-CD3 bispecific engager molecules has shown promise in clinical studies for CD19+ malignancies. However engager molecules have short half-lives and do not accumulate at tumor sites. In addition, co-delivery of other immunostimulatory molecules to enhance antitumor effects is difficult to achieve. We have recently shown that T cells can be genetically modified to secrete bispecific engager molecules (ENG-T cells). ENG-T cells are activated by tumor cells in an antigen-dependent manner, redirect bystander T cells to tumor cells, and have antitumor activity in preclinical models. We now wanted to explore if additional genetic modifications of ENG-T cells can enhance their effector function in vitro and in vivo. Since bispecific engager molecules do not provide co-stimulation, we focused on the provision of co-stimulatory signals by coexpressing CD80 and CD137L on the cell surface of ENG-T cells. Thus, the aim of the study was to compare the effector function of CD19-specific T-cell engagers (CD19-ENG T cells) and CD19-ENG T cells co-expressing CD80 and 41BBL (CD19-ENG/Costim T cells). Methods: CD19-ENG T cells were generated by transducing T cells with a retroviral vector encoding a CD19-specific T-cell engager and mOrange separated by an IRES (SFG.CD19-ENG-I-mO), and CD19-ENG/Costim T cells were generated by double transducing T cells with SFG.CD19-ENG-I-mO and a 2nd retroviral vector encoding 41BBL and CD80 separated by an IRES. The effector function of ENG T-cells was evaluated in vitro and in a leukemia xenograft model. Results: After single or double transduction 60-80% of T cells were positive for mOrange, and ~80% of CD19-ENG/Costim T cells were positive for CD80 and 30-40% positive for 41BBL. In coculture assays CD19-ENG and CD19-ENG/Costim T cells recognized CD19+ lymphoma (Daudi, Raji) and acute leukemia (BV173) cells as judged by IFN-g secretion in contrast to negative controls. While CD19+ target cells that express CD80 and CD86 (Daudi and Raji) induced robust IL2 production of CD19-ENG and CD19-ENG/Costim T cells, CD19-ENG/Costim T cells produced significantly higher levels of IL2 in comparison to CD19-ENG T cells after stimulation with CD19+/CD80-/CD86- negative target cells (BV173). Cytokine production was antigen dependent since ENG and ENG/Costim T cells specific for an irrelevant antigen (EphA2) did not produce cytokines. Specificity was confirmed in cytotoxicity assays. In transwell assays containing inserts preventing T-cell migration, only ENG T cells redirected bystander T cells in the bottom well to CD19+ tumor cells. To assess in vivo anti-tumor activity of CD19-ENG T cells and CD19-ENG/Costim T cells we used the BV173/NSG mouse xenograft model in which BV173 cells are genetically modified with firefly luciferase (ffLuc-BV173) to allow for serial bioluminescence imaging. While therapy with CD19-ENG T cells on day 7 post ffLuc-BV173 injection resulted in the cure of all mice, when therapy was delayed to day 14, only 1/10 mice was alive on day 80. In contrast therapy of mice on day 14 with CD19-ENG/Costim T cells resulted in long-term survival of 7/10 mice. Control T cells (EphA2-ENG T cells or EphA2-ENG/Costim T cells) had no antitumor effects. Conclusions: We have generated CD19-ENG T cells and CD19-ENG/Costim T cells with the ability to direct bystander T cells to CD19+ malignancies. Both ENG T-cell populations had potent antitumor activity in a preclinical ALL model, and provision of costimulation further enhanced antitumor effects. Genetically modifying T cells to express engager molecules and additional molecules to enhance their effector function may present a promising alternative to current CD19-targeted immunotherapies. Disclosures Velasquez: Celgene, Bluebird bio: Other. Iwahori:Celgene, Bluebird bio: Other. Kakarla:Celgene, Bluebird bio: Other. Song:Celgene, Bluebird bio: Other. Gottschalk:Celgene, Bluebird bio: Other.
10
Mu-Mosley, Hong, Lauren B. Ostermann, Muharrem Muftuoglu, Wendy Schober, Nalini B. Patel, Abishek Vaidya, Challice L. Bonifant, Stephen Gottschalk, Mireya Paulina Velasquez, and Michael Andreeff. "Transgenic Expression of IL15 in CD123-Specific BiTE-Secreting Engager T-Cells Results in Improved Anti-AML Activity." Blood 134, Supplement_1 (November 13, 2019): 3917. http://dx.doi.org/10.1182/blood-2019-125928.
Full textAbstract:
Background: CD123 is frequently expressed on hematologic malignancies including 96-98% of AML. CD123 has been a potential immunotherapeutic target in AML due to its association with leukemic stem cells that play an essential role in disease progression and relapse. Our previous study using T-cells secreting CD123/CD3-bispecific T-cell engagers (BiTEs) (CD123-ENG T-cells) showed a promising approach anti-AML activity, however T-cell persistence was limited. Interleukin-15 (IL15) has emerged as a candidate immunomodulator as it enhances T-cell expansion and persistence, and induces long-lasting memory T-cells. To improve the efficacy and persistence of CD123-ENG T-cells we developed IL15 expressing CD123-ENG T-cells. Here, we report on the characterization and efficacy of IL15-secreting CD123-ENG T cells in vitro and in vivo models of adult AML. Methods/Results: A cDNA encoding IL15 was cloned into retroviral vectors encoding CD123-ENG or CD19-ENG (CD123-ENG.IL15; CD19-ENG.IL15). ENG T-cells were generated from human peripheral blood mononuclear cells (PBMCs) from normal donors or T-cells from AML patients by retroviral transduction and in vitro expansion. Non-transduced (NT) T-cells and T-cells expressing CD123 (CD123-ENG T-cells) served as controls. IL15 production of CD19-ENG.IL15 and CD123-ENG.IL15 T cells was confirmed by ELISA (144-159 pg/ml vs 38 and 46 pg/ml of NT and CD123-ENG T cells, p<0.01, n=6). Both CD123-ENG and CD123-ENG.IL15 T-cells recognized CD123+ AML cells as judged by IL2 and interferon γ (IFNγ) production (p<0.01, n=5). In contrast, NT and CD19-ENG.IL15 T-cells did not, confirming specificity. In addition, CD123-ENG.IL15 and CD123-ENG T-cells induced killing of only CD123-positive target cells as well as of primary adult patients' AML blasts in luciferase- or 7AAD-based cytotoxicity assays (p<0.001, n=10). CD123-ENG.IL15 T-cells showed greater cytolytic activity than CD123-ENG T-cells as determined by luciferase activity (p=0.0002, n=3). In a Molm13 AML xenograft model, CD123-ENG.IL15 and CD123-ENG T-cells exhibited potent anti-leukemic activity as judged by bioluminescence imaging. Moreover, CD123-ENG.IL15 T cells had enhanced anti-leukemic activity and greater persistence in BMs, spleens, and livers in comparison to CD123-ENG T cells, resulting in improved anti-AML activity (Figure 1, p<0.01 vs CD123-ENG T-cell group, n=12 per group) and extended survival (Figure 2, p=0.0097 vs CD123-ENG T-cell group). Finally, AML PDX models and ENG T-cells were generated from AML blasts and T-cells from 3 patients with active AML. Infusion of autologous ENG T-cells (1.5x106 cells/mouse, n=7) in AML PDX#6697688 mouse model revealed significant reduction of leukemia burden in the CD123-ENG.IL15 or CD123-ENG T-cells mouse groups but not in the mouse group with NT or CD19-ENG.IL15 T-cells or PBS (p=0.004, n=6-8). We are currently monitoring survival of these PDX models. Conclusion: We here demonstrated that transgenic expression of IL15 in CD123-ENG T-cells results in improved expansion and persistence, and anti-AML activity. These results warrant further exploration of IL15-modified CD123-targeted T-cells as immunotherapy for AML. Disclosures Bonifant: Patents filed in the field of engineered cellular therapies: Patents & Royalties: Patents filed in the field of engineered cellular therapies. Gottschalk:EMD Serono: Honoraria; Inmatics: Membership on an entity's Board of Directors or advisory committees; ASSISI fundation of Memphis: Research Funding; TESSA Therapeutics: Other: Research Collaboration; ViraCyte: Consultancy; NHLBI: Research Funding; America Lebanese Syrian Associated Charities: Research Funding; California Institute for Regenerative Medicine: Research Funding; Patents and patent applications in the fields of T-cell & Gene therapy for cancer: Patents & Royalties; MBIO: Other: St. Jude Children's Research Hospital has an existing exclusive license and ongoing partnership with Mustang Bio for the further clinical development and commercialization of this XSCID gene therapy; Sanofi: Honoraria; Tidal: Membership on an entity's Board of Directors or advisory committees; Merck: Consultancy. Velasquez:St. Jude: Patents & Royalties: Patent Applications in the Fields of Cell and Gene Therapy ; Rally! Foundation: Membership on an entity's Board of Directors or advisory committees. Andreeff:Senti Bio: Equity Ownership, Membership on an entity's Board of Directors or advisory committees; AstaZeneca: Consultancy; Amgen: Consultancy; Eutropics: Equity Ownership; Aptose: Equity Ownership; Reata: Equity Ownership; 6 Dimensions Capital: Consultancy; Celgene: Consultancy; Jazz Pharmaceuticals: Consultancy; Daiichi Sankyo, Inc.: Consultancy, Patents & Royalties: Patents licensed, royalty bearing, Research Funding; Cancer UK: Membership on an entity's Board of Directors or advisory committees; NCI-CTEP: Membership on an entity's Board of Directors or advisory committees; German Research Council: Membership on an entity's Board of Directors or advisory committees; Leukemia Lymphoma Society: Membership on an entity's Board of Directors or advisory committees; NCI-RDCRN (Rare Disease Cliln Network): Membership on an entity's Board of Directors or advisory committees; CLL Foundation: Membership on an entity's Board of Directors or advisory committees; BiolineRx: Membership on an entity's Board of Directors or advisory committees; NIH/NCI: Research Funding; Center for Drug Research & Development: Membership on an entity's Board of Directors or advisory committees; CPRIT: Research Funding; Oncoceutics: Equity Ownership; Oncolyze: Equity Ownership; Breast Cancer Research Foundation: Research Funding.
Dissertations / Theses on the topic "Survival. eng"
1
Tanaka, Fábio Yoshio. "Estudo da viabilidade celular comparando os meios de conservação para enxerto ósseo de calota craniana : análise microscópica e imunoistoquímica em ratos /." Araçatuba : [s.n.], 2005. http://hdl.handle.net/11449/101040.
Full textAbstract:
Resumo: O objetivo deste trabalho foi analisar a viabilidade celular comparando os meios de conservação para enxerto ósseo. A preservação de células viáveis em procedimentos de enxerto ósseo é de fundamental importância para que se tenha a osteogênese. Foram utilizados 43 ratos machos. Após a antissepsia do campo operatório foi realizada incisão linear na região mediana da calota craniana para obtenção do enxerto da região parietal direita e esquerda as quais foram removidas com auxílio de trefina de 5mm de diâmetro acoplada em micro-motor de baixa rotação, sob constante irrigação com solução de soro fisiológico 0,9% estéril. As peças do enxerto foram acondicionadas em tubos de ensaio estéreis os quais foram devidamente identificadas de acordo com o grupo e mantidas dentro deste tubo conforme cada condição do grupo. Como meio de conservação da viabilidade celular do enxerto foi utilizado o soro fisiológico a 0,9% (Grupo I) e a solução de Euro Collins® (Grupo II) e ainda para verificar se a temperatura tem influência direta na manutenção da viabilidade celular foi analisado o enxerto ósseo conservado em temperatura ambiente (Grupo III) e o enxerto ósseo sem nenhuma solução, porém mantido em gelo (GrupoIV). Para avaliar a viabilidade celular foi utilizada análise histológica e imunoistoquímica imediata e ainda em cada grupo analisou-se a viabilidade celular no período de 6 horas, 12 horas, 24 horas e 30 horas. Como resultado observou-se que a solução de Euro Collins® apresentou-se superior ao soro fisiológico no que se diz respeito à manutenção da viabilidade celular do enxerto ósseo onde se pode notar viabilidade celular até o período de 30 horas.Abstract: The aim of this study was to analyze cellular viability comparing storage media for skull vault bone graft. Preservation of viable cells in bone graft procedures is of paramount importance to obtain osteogenesis. Forty-three male used in this study. After antisepsis of the operative field, a linear incision was made on the middle region of the skull vault to obtain a bone graft from the right and left parietal areas. The grafts were removed with a 5-mm diameter trephine bur coupled to low-speed handpiece under continuous irrigation with sterile 0.9% saline. The graft pieces were placed in sterile 5-mL test tubes with caps, and were properly identified according to the group and maintained inside the test tubes as per each group conditions. The storage media evaluated for preservation of graft cellular viability were 0.9% saline (Group I) and Euro Collins® solution (Group II). In order to assess whether the temperature has a direct influence on the maintenance of cellular viability, the analysis was extended to bone grafts stored at room temperature (Group III) and bone grafts with no solution, but maintained in ice (Group IV). Cellular viability was evaluated by immediate histological and immunohistochemical analyses. For each group, cellular viability was analyzed at 6, 12, 24 and 30 hours after procedure. The results of this study showed that Euro Collins® solution yielded better performance than 0.9% saline as regards the maintenance of bone graft cellular viability (up to 30 hours).
Orientador: Tetuo Okamoto
Coorientador: Roberta Okamoto
Banca: Luis Eduardo Marques Padovan
Banca: Hugo Nery Filho
Banca: Celso Koogi Sonoda
Banca: Idelmo Rangel Garcia Júnior
Banca: Wilson Roberto Poi
Doutor
2
Zuque, Maria Angelina da Silva. "Caracterização do atendimento multiprofissional a pessoas vivendo com HIV/AIDS em Três Lagoas (MS), com ênfase na adesão à terapia antirretroviral potente combinada /." Botucatu : [s.n.], 2011. http://hdl.handle.net/11449/89973.
Full textAbstract:
Orientador: Carlos Magno Castelo BrancoBanca: Ricardo Augusto Monteiro de Barros Almeida
Banca: Rosely Morales de Figueiredo
Resumo: Quase três décadas após o primeiro diagnóstico no Brasil, a aids demonstra tendência contínua de interiorização, com aumento da incidência em pequenos municípios. A implantação de Serviços de Atendimento Especializado (SAE's) foi marco importante para o sucesso das ações programáticas em cidades de pequeno porte. Curiosamente, a maior parte dos estudos epidemiológicos e de avaliação dos serviços tem sido realizada em grandes centros urbanos. Este trabalho teve por objetivo caracterizar o atendimento multiprofissional a pessoas vivendo com HIV/AIDS (PVHA) realizado no SAE de Três Lagoas (MS), uma cidade de aproximadamente 100.000 habitantes. A autora estava especialmente interessada em aspectos relacionados à adesão à Terapia Antirretroviral Potente e Combinada (TARV), condição essencial para redução de morbidade e mortalidade de PVHA. Dois estudos foram realizados. No primeiro (Estudo No.1), foram analisados prontuários e fichas de notificação de 356 pacientes admitidos entre 1984 e 2005, para avaliar características demográficas. Os dados foram analisados para dois períodos: 1984-1995, fase em que a TARV não era utilizada na prática clínica (pré-TARV); e 1996 e depois (pós-TARV), quando a introdução de novos medicamentos permitiu a utilização da TARV no âmbito do Sistema Único de Saúde (SUS). Realizou-se também análise de sobrevida, para avaliar o impacto da disponibilização da TARV e de outros fatores sobre o prognóstico dos pacientes. O Estudo No.2 teve delineamento quali-quantitativo, e baseou-se em entrevistas com 55 pacientes e sete profissionais da saúde de diversas categorias. Os resultados do Estudo No.1 demonstraram que dois terços dos pacientes foram admitidos no período pós-TARV. Nessa fase, aumentaram os casos no sexo feminino (22,0% para 36,2%, p=0,02) e em pessoas com escolaridade inferior a ensino fundamental completo... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Nearly three decades after the first diagnosis in Brazil, AIDS demonstrates the continuing trend of internalization, with increasing incidence in small towns. Deployment of Specialized Care Services (SCS) was important to the success of Brazilian aids program in small cities. Interestingly, most epidemiological studies and evaluation of services have been conducted in large urban centers. This study aimed to characterize the multidisciplinary care for people living with HIV/AIDS (PLWHA) held at SCS in Três Lagoas (State of Mato Grosso do Sul, Brazil), a city of about 100,000 inhabitants. The author was especially interested in aspects related to adherence to Highly Active Antiretroviral Therapy (HAART), an essential condition for reducing morbidity and mortality among PLWHA. Two studies were conducted. In the first study (Study #1), the author analyzed medical records and notification forms for 356 patients admitted between 1984 and 2005 in order to assess demographic characteristics. Data were analyzed for two periods: 1984-1995, a phase in which HAART was not used in clinical practice (pre-HAART) and 1996 and later (post-HAART), when the introduction of new drugs has allowed the use of HAART in the Brazilian Public Health System. Survival analysis was applied to assess the impact of the availability of HAART and other factors on the prognosis of patients. Study #2 had a qualitative and quantitative design. The author interviewed 55 patients and seven health professionals from various categories. The results of Study #1 showed that two thirds of patients were admitted in the post-HAART period. At this stage, cases increased in females (22.0% to 36.2%, p=0.02) and among people with less than eight years of schooling (61.3% to 72.3%, p<0.001 ). The mortality risk was lower for patients admitted in the post-HAART (Hazard Ratio [HR] = 0.31, 95% Confidence Interval[CI] = 0.22 to 0.45,... (Complete abstract click electronic access below)
Mestre
3
Cardoso, Sandra Regina de Sousa 1973. "Morfogênese de ninhos iniciais de atta spp. (Hymenoptera: formicidae), mortalidade em condições naturais e avaliação da ação de fungos entomopatogênicos /." Botucatu : [s.n.], 2010. http://hdl.handle.net/11449/105448.
Full textAbstract:
Orientador: Luiz Carlos FortiBanca: Carlos Frederico Wilcken
Banca: Ana Paula Protti de Andrade Crusciol
Banca: Aldenise Alves Moreira
Banca: Sandra Verza da Silva
Resumo: As formigas cortadeiras são consideradas pragas importantes para diversas plantas cultivadas, o que exige constante controle. No entanto, a mortalidade natural de colônias iniciais de Atta spp é alta, alcançando até 100%, no primeiro ano de fundação. Dentre vários fatores os micro-organismos patógenos são apontados como um dos responsáveis por tal mortalidade. Assim, o presente estudo teve como objetivo: (1) estudar a morfogênese dos ninhos iniciais em função do tempo de fundação essas espécies; (2) avaliar a taxa de mortalidade de rainhas de Atta bisphaerica e Atta sexdens rubropilosa, durante os primeiros meses de fundação; (3) isolamento e caracterização dos fungos entomopatogênicos de ocorrência natural em rainhas; (4) realizar bioensaios para avaliar a patogenicidade desses isolados em operárias de Atta sexdens rubropilosa e (5) formulação e aplicação de iscas à base de isolados de fungos entomopatogênicos, em colônias de Atta sexdens rubropilosa, visando possibilidades de aplicação prática. Para tal, foram coletadas rainhas recém-fecundadas ou provenientes de escavações dos ninhos incipientes marcados no período da revoada. As escavações ocorreram aos 20, 45, 90, 135, 180 e 225 dias de idade do ninho em 2007, e aos 20, 45 e 90 dias em 2008, e aos 60 dias em 2009. Foram obtidos os seguintes resultados: (1) as dimensões da câmara-recém fundada aumentam com o tempo de fundação; (2) altas taxas de mortalidade para A. bisphaerica e A. sexdens rubropilosa; (3) rainhas de Atta spp. podem ser parasitadas por diversos fungos, dentre eles Beauveria bassiana, Isaria lilacinus, Isaria fumosorosea e Aspergillus flavus; (4) todos os isolados são patogênicos e os mais virulentos BBOT11, BBOT12 de B. bassiana e IBOT33 de I. lilacinus; e (5) iscas formuladas com fungos são atrativas, carregadas e incorporadas por operárias de A. sexdens rubropilosa, contudo não causam
Abstract: The leaf-cutting ants are pests for several important crop plants, which requires constant control. However, the natural mortality of Atta spp colonies is high, reaching 100% in the first year of foundation. Among many factors the pathogenic microorganisms are noted as one of those responsible for such mortality. Thus, this study aimed to: (1) study the morphogenesis of early nests depending on the time of foundation of these species; (2) assess the death rate of the queens of Atta bisphaerica and Atta sexdens rubropilosa during the first months of the foundation, through excavations in newly founded nests; (3) isolation and characterization of entomopathogenic fungi found in queens; (4) perform bioassays to assess the pathogenicity of these isolates in Atta sexdens rubropilosa and (5) formulation and application of baits based on entomopathogenic fungi isolated in colonies of Atta sexdens rubropilosa, seeking opportunities for practical application. To this end, we collected newly fertilized queens or from excavations of incipient nests marked during the time of nuptial flight. The excavations were performed at 20, 45, 90, 135, 180 and 225 days of nest age in 2007, and at 20, 45 and 90 days in 2008. The results were: (1) the dimensions of the incipient chamber increases with time of the foundation; (2) high rates of mortality for Atta bisphaerica and Atta sexdens rubropilosa; (3) queens of Atta spp. can be parasitized by several fungi, including Beauveria bassiana, Isaria lilacinus, Isaria fumosorosea and Aspergillus flavus; (4) all strains are pathogenic and the most virulent BBOT11, BBOT12 of B. bassiana and IBOT33 of I. lilacinus; and (5) baits made with fungi are attractive, loaded and incorporated by workers of A. sexdens rubropilosa, yet not cause mortality of the colony
Doutor
4
Massago, Haluko. "Desempenho de alevinos de quatro linhagens da tilápia do Nilo (Oreochromis niloticus) e análise da variabilidade genética pelos marcadores RAPD /." Jaboticabal : [s.n.], 2007. http://hdl.handle.net/11449/86688.
Full textAbstract:
Orientador: Newton CastagnolliBanca: João Batista Kochenborger Fernandes
Banca: Ricardo Pereira Ribeiro
Resumo: Este trabalho teve como objetivo verificar o desempenho inicial de quatro linhagens comerciais de Oreochromis niloticus denominadas "Bouaké", "GIFT", "Supreme" e "Chitralada", com ênfase no ganho em peso e sobrevivência. Utilizou-se os alevinos revertidos com o peso médio de um grama. O experimento foi conduzido em sistema fechado durante 112 dias, com biometrias realizadas a cada 28 dias. Inicialmente eram mantidos 38 peixes por 120 L em cada caixa e, após 84 dias, 13 peixes por 180 L. A qualidade da água foi avaliada através dos seguintes parâmetros: temperatura, oxigênio dissolvido (OD) e Potencial Hidrogeniônico (pH). Forneceu-se inicialmente a ração em pó com 45% de proteína bruta e, depois, a ração extrusada contendo 40% do mesmo nutriente. Os pesos médios finais das linhagens "Bouaké", "GIFT", "Supreme" e "Chitralada" foram 98,83 g, 121,46 g, 133,20 g e 112,89 g, respectivamente. As linhagens "Supreme" e "GIFT" apresentaram melhor desempenho, sendo que o desempenho da linhagem "GIFT" foi semelhante ao da "Chitralada". O desempenho da linhagem "Bouaké" não diferiu da "Chitralada" (p<0,05). A taxa de sobrevivência (acima de 80%, com exceção da "Bouaké") pode ser considerada normal.
Abstract: This work aimed to verify the initial performance of four commercial Oreochromis niloticus strains named "Bouaké", "GIFT", "Supreme" and "Chitralada", with special attention on the weight gain and survival rates. Tilapia fingerlings, post reversion with mean weight of one gram were used. The experiment was carried out in a closed system of Tilapia laboratory of CAUNESP, during 112 days, and biometries for performance evaluation have been done every 28 days. Water quality was evaluated through the following parameters: temperature, dissolved oxygen and pH value. Feeding was initially with powdered ration with 45 % crude protein, and after three weeks, with extruded ration with 40% crude protein. The final mean weight of the "Bouaké", "GIFT", "Supreme" and "Chitralada" strains were 98.83g, 121.46 g, 133.20 g and 112.89 g, respectively. "Supreme" and "GIFT" strains presented better performance, and the "GIFT" and "Chitralada" strains were similar too (p<0.05). Survival rate was considered normal (above 80%), with the exception of "Bouaké" strain.
Mestre
5
Anita, Schmidek. "Variabilidades genética e não genética na mortalidade pré-desmama de bezerros de corte /." Jaboticabal : [s.n.], 2009. http://hdl.handle.net/11449/102781.
Full textAbstract:
Resumo: Os objetivos desta pesquisa foram os de ampliar os conhecimentos sobre os efeitos genéticos e não genéticos da mortalidade pré-desmama em bovinos de corte no Brasil, bem como avaliar a possibilidade de incluir características diretas e/ou indicativas na avaliação genética de reprodutores. Foram estudadas bovinos das raças Nelore (dois rebanhos) e Guzerá (um rebanho). Os registros de mortalidade pré-desmama, vigor ao nascimento e falhas na primeira mamada foram analisados utilizando a metodologia de modelos lineares generalizados com o intuito de avaliar efeitos não genéticos, e a metodologia de modelos lineares generalizados mistos foi utilizada para as análises genéticas. Baixo vigor e pesos ao nascer extremos foram os efeitos que mais influenciaram as características estudadas. As estimativas de herdabilidade considerando o bezerro foram entre 0,05 e 0,27 para mortalidade, 0,10 para vigor e nula para falha na primeira mamada. A estimativa de herdabilidade considerando a vaca foi 0,17 para falhas na primeira mamada. A seleção praticada para peso ao sobreano não influenciou a mortalidade pré-desmama. A variação na ocorrência de óbitos durante o período pré-desmama indica que as inspeções aos bezerros devam ser mais frequentes durante a primeira quinzena de vida, embora devam ser realizadas com frequência durante toda a fase pré-desmama. Visando detectar bezerros com maior risco de óbito, é recomendado atentar aos fatores de maior influência para baixo vigor e mortalidade, que corresponderam a bezerros muito leves ou muito pesados, filhos de primíparas ou de vacas velhas, bezerros do sexo masculino. O registro do vigor ao nascimento pode ser adotado como ferramenta para evitar que óbitos ocorram, pois é de baixo custo e apresenta coleta simples e confiável... (Resumo completo, clicar acesso eletrônico abaixo)Abstract: The aim of this research was to increase the knowledge about genetic and non genetic effects upon pre-weaning Brazilian beef cattle mortality, as well as to evaluate the possibility of considering direct and/or indicative characteristics in genetic evaluation of sires. Herds of Nelore (2) and Guzerá (1) cattle were studied assessing pre-weaning mortality, calf vigor at birth and neonate behaviour; all records were analyzed using the methodology of generalized linear models regarding non genetic effects, and the methodology of generalized linear mixed models was used for the genetic analysis. Calf birth weight was the main effect influencing calf vigor and mortality, with more problems occurring in calves with extreme weights, especially the very low ones. The estimated direct heritabilities were low to moderate for calf mortality (0.05 to 0.27), low for vigor (0.10) and null for the fails in the first suckling. Maternal heritabilities were low for calf mortality and vigor (0.00 to 0.03) and moderate for the fails in the first suckling (0.17). The selection for yearling weight did not influence pre-weaning mortality. The variation in deaths occurrence during the pre-weaning period indicates that inspections should be more intense during the first fifteen days of life. Considering the calves with high risk of death, it is recommended to pay attention to the factors that induce the low vigor and mortality of calves, eg. very thin and heavy calves, calves born from heifers or old cows, as well as male calves. The register of vigor at birth can be adopted as a tool to prevent that deaths occur, since it is of low cost and presents simple and trustworthy collection for prediction of calves with death risk. Due the importance of the mammary system, affecting the frequency of fails in the first suckling, it is expected... (Complete abstract click electronic access below)
Orientador: Mateus José Rodrigues Paranhos da Costa
Coorientadora: Maria Eugênia Zerlotti Mercadante
Banca: Joslaine Noeli dos Santos Gonçalves Cyrillo
Banca: Pedro Franklin Barbosa
Banca: Lucia Galvão de Albuquerque
Banca: Henrique Nunes de Oliveira
Doutor
6
Del'Arco, Marcelo. "Biologia de Tenuipalpus heveae Baker (acari, Tenuipalpidae) em três clones de Fevea brasileinsis Muell. Arg. (Euphorbiaceace) /." São José do Rio Preto : [s.n.], 2008. http://hdl.handle.net/11449/87578.
Full textAbstract:
Orientador: Reinaldo José Fazzio FeresBanca: Marineide Rosa Vieira
Banca: Marcel Ricardo Tanzini
Resumo: Esse trabalho teve como objetivo estudar o ciclo biológico de T. heveae em folíolos de seringueira dos clones RRIM 600, PB 235 e GT 1. Inicialmente foi estabelecida uma colônia de manutenção de T. heveae. As arenas de criação e para estudos da biologia foram confeccionadas a partir de folíolos de seringueira dos clones acima citados sendo mantidas sob condições controladas em câmara climatizada do tipo BOD com umidade relativa de 70+10%, fotoperíodo de 12 h, 28+0,1oC na fotofase e 25+0,1oC na escotofase. Três observações diárias foram realizadas, acompanhando-se o desenvolvimento de 60 ovos em folíolos de cada clone para verificação do estágio de desenvolvimento em que o ácaro se encontrava, assim como a oviposição das fêmeas. A fase mais longa registrada nos três clones foi a de ovo e o período mais curto foi o protoninfal. Em todos os clones estudados a fase de maior viabilidade foi a deutoninfal, e a menor, a larval. Quatorze fêmeas e quatro machos atingiram a fase adulta no clone PB 235, sendo observada uma taxa média de oviposição de 1,2 ovo/dia/fêmea. No clone RRIM 600, nove fêmeas e dois machos foram observados, sendo registrada a taxa de oviposição de 0,7 ovo/dia/fêmea. No clone GT 1, sete fêmeas chegaram à fase adulta com taxa média de 0,51 ovo/dia/fêmea. O ciclo completo da incubação do ovo até a morte do adulto durou 25 dias, em média, nos clones RRIM 600 e PB 235. A taxa intrínseca de crescimento populacional (rm) foi de 0,09; 0,08 e 0,02 nos clones PB 235, RRIM 600 e GT 1, respectivamente. O clone PB 235 possibilitou um melhor desenvolvimento de T. heveae, seguido pelo clone RRIM 600. O clone GT 1 foi o menos favorável ao desenvolvimento.
Abstract: This work aims to study the biological cycle of T. heveae in rubber tree leaflets of clones RRIM 600, PB235 and GT 1. Initially, a maintenance colony was established. The breeding arenas and those for the study of the biology were created using leaflets of the aforementioned rubber tree clones, which had been kept under controlled conditions in a climatic chamber. Three daily observations were done, following the development of 60 eggs in each clone to check the developmental stage and female oviposition. The egg was the longest-lasting phase registered and the protonymph was the shortest period. The deutonymph was the most viable phase in all clones, whereas the least viable was the larval stage. Fourteen females and four males reached the adult stage in the clone PB 235, an average oviposition rate of 1.2 egg per day per female. In the RRIM 600 clone, nine females and two males were viable, and an oviposition rate of 0.70 egg per day per female was observed. In the GT 1 clone, seven females reached the adult stage, the average ovoposition rate was 0.51 egg per day per female. The complete cycle of incubation of the egg until the death of the adult lasted, on average, 25 days in RRIM 600 and PB 235 clones. The intrinsic rate of population increase (rm) was 0.09; 0.08 and 0.02 in clones PB 235, RRIM 600 and GT1, respectively. The clone PB 235 allowed a better development of T. heveae, followed by RRIM 600. The clone GT 1 was the least favourable for the development of that mite species.
Mestre
7
Ambrósio, Márcia Michelle de Queiroz 1976. "Sobrevivência em microcosmo e em campo solarizado de fitopatógenos submetidos à fermentação acelerada de diferentes matériais orgânicos /." Botucatu : [s.n.], 2006. http://hdl.handle.net/11449/105412.
Full textAbstract:
Orientador: Nilton Luiz de SouzaBanca: Edson Luiz Furtado
Banca: Roberto Lyra Villas Bôas
Banca: Alfredo Seiti Urashima
Banca: Flávia Rodrigues A. Patricio
Resumo: A solarização é uma das alternativas de controle físico para vários fitopatógenos que podem sobreviver por vários anos no solo por meio de estruturas de resistência, causando danos em muitas culturas, e muitas vezes inviabilizando vastas áreas agrícolas. No entanto, existem algumas espécies que possuem temperatura letal acima daquela atingida pela solarização do solo. Vem sendo estudado a incorporação de material orgânico previamente a colocação do plástico, situação esta que promove o aprisionamento dos gases oriundos da decomposição, conferindo um efeito aditivo à solarização. O objetivo do presente trabalho consistiu na prospecção de materiais orgânicos promissores para produzir voláteis fungitóxicos capazes de controlar fitopatógenos de solo. Foram realizados dois ensaios em condições de microcosmo (câmara de vidro) para simular a solarização do solo, onde incorporou-se 1kg/m2 de material orgânico fresco e posteriormente, dois experimentos de campo, em épocas diferentes e em áreas contíguas com os mesmos materiais orgânicos. No primeiro experimento foi incorporado 1kg/m2 de material orgânico e no segundo 3Kg/m2. Estudou-se quatro fitopatógenos (Fusarium oxysporum f. sp. lycopersici Raça 2; Macrophomina phaseolina; Rhizoctonia solani AG-4 HGI e Sclerotium rolfsii) frente a quatro 2 materiais orgânicos frescos e triturados incorporados ao solo (folhas e ramos de brócolos, eucalipto, mamona e mandioca brava). O controle dos fungos foi avaliado por meio da análise da sobrevivência das estruturas em meio semi-seletivo específicos, durante quatro períodos (7, 14, 21 e 28 dias do início do experimento). Nos experimentos de campo, foram monitoradas a temperatura por um coletor de dados Tipo CR23X (Campbell Scientific) e a porcentagem de CO2 e de O2 pelo equipamento analisador de gases (Texto 325-1)... (Resumo completo, clicar acesso eletrônico abaixo).
Abstract: Solarization is one of the physical control alternatives available against a number of plant pathogens capable of surviving for several years in the soil by means of resistance structures, causing damage to many crops, and often rendering vast agricultural areas unviable. However, there are a few species whose lethal temperature is above the temperature reached during soil solarization. The incorporation of organic material prior to mulching with plastic is currently being studied. This condition promotes the trapping of gases from decomposition, imparting an additive effect to solarization. The objective of the present work consisted in the investigation of organic materials which are promising to produce fungitoxic volatiles that can control soil plant pathogens. Two assays were carried out under microcosmic conditions (glass chamber) to simulate soil solarization, in which 1kg/m2 of fresh organic material were incorporated. Later, two field experiments were carried out during different seasons and at contiguous areas, using the same organic materials. In the first experiment, 1kg/m2 of organic material was incorporated, while 3Kg/m2 were incorporated in the second. Four plant pathogens (Fusarium oxysporum f. sp. lycopersici Strain 2; Macrophomina phaseolina; Rhizoctonia solani AG-4 HGI, and Sclerotium rolfsii) were studied under four types of fresh, ground organic materials incorporated into the soil (leaves and branches of broccoli, eucalyptus, castor bean, and wild cassava). Fungus control was evaluated through the analysis of the survival of structures in a specific semi-selective medium, during four periods (7, 14, 21, and 28 days from the beginning of the experiment). In 4 the field experiments, temperature was monitored with a Type CR23X (Campbell Scientific) data collector, and CO2 and O2 percentages were monitored with a Testo 325-1 gas analyzer equipment. In both field experiments...(Complete abstract, click electronic address below)
Doutor
8
Silva, Júnior Tadeu Antônio Fernandes da 1982. "Curtobacterium flaccumfaciens PV. flaccumfaciens: sobrevivência, gama de hospedeiras e efeito do pré-plantio de aveia e trigo na ocorrência da doença /." Botucatu, [s.n.], 2011. http://hdl.handle.net/11449/105411.
Full textAbstract:
Orientador: Antonio Carlos MaringoniBanca: Marli Teixeira de Almeida Minhoni
Banca: Renate Krause Sakate
Banca: Luís Otavio Saggion Beriam
Banca: Ivan Paulo Bedendo
Resumo: A murcha-de-curtobacterium, causada por Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), é uma das principais doenças bacterianas da cultura do feijoeiro, acarretando grandes perdas na produção dessa cultura. Até o momento existem poucas informações sobre os diferentes nichos de sobrevivência desta bactéria e de sua gama de hospedeiras. Em vista disso, este trabalho teve por objetivos principais verificar a capacidade de sobrevivência saprofítica de Cff em restos de cultura de feijoeiro mantidos na superfície do solo e enterrados à 20 cm de profundidade; a influência da temperatura, umidade e do tipo de solo no período de sobrevivência da bactéria em solo; determinar a gama de hospedeiras de Cff inoculadas artificialmente, tanto por ferimento no caule, como por aspersão de suspensão bacteriana na parte aérea das plantas; a capacidade de colonização de Cff do rizoplano de plantas de aveia e trigo; e o efeito do pré-plantio de aveia e trigo na ocorrência da murcha-de-curtobacterium. Quanto à capacidade de sobrevivência de Cff em restos de cultura de feijoeiro, foi demonstrado que a bactéria possui menor capacidade de sobrevivência quando os restos vegetais são incorporados ao solo e também em épocas com maiores índices de precipitação e temperaturas mais altas. O período de sobrevivência do patógeno nos restos culturais de feijoeiro mantidos na superfície do solo variou entre 165 e 240 dias e nos restos vegetais enterrados a 20 cm de profundidade, o período de sobrevivência foi inferior a 30 dias. Quanto à sobrevivência de Cff na forma de células livres no solo, foi verificado que a temperatura, a umidade e o tipo do solo têm influencia na capacidade de sobrevivência da bactéria. O tempo de sobrevivência de Cff variou entre dois e quinze dias. Das 30 espécies botânicas inoculadas artificialmente com Cff, a bactéria causou lesões na parte... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Bacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is one of the main bacterial diseases affecting bean culture, leading to great losses in its production. So far there is scarce information about the different survival niches and host range of this bacterium. Thus, the present study had as major aims to verify Cff capability of saprophytically surviving in bean debris kept on the soil surface and buried at 20 cm depth; to assess the influence of temperature, humidity and soil type on the survival period of this bacterium in soil; to determine the host range for artificially inoculated Cff, either through stem injury or through bacterial sprinkling onto the shoot of plants; to verify Cff capability of colonizing the rhizoplane of oat and wheat plants; and to assess the effect of oat and wheat pre-planting on the occurrence of bean bacterial wilt. Cff had decreased capability of surviving in bean debris when the latter were incorporated into the soil and during periods of higher rainfall rates and temperatures. The pathogen survival period in bean culture remnants kept on the soil surface ranged from 165 and 240 days, while in plant debris buried at 20 cm depth the survival period was inferior to 45 days. The survival capability of Cff as free cells in soil was influenced by temperature, humidity and soil type. Cff survival time varied between two and fifteen days. Of 30 plant species artificially inoculated with Cff, bean and soy shoot had lesions caused by the bacterium which endophytically colonized wheat leaves and soy and wheat stem and leaves. Cff was also shown to have no capability of colonizing oat and wheat rhizoplane while the pre-planting of these grass plants, before bean culture establishment, had no effect on the occurrence of bean bacterial wilt
Doutor
9
Galeazzi, Priscilla Markarian. "Efeitos genéticos e ambientais da habilidade de permanência de búflas leiteiras da raça Murrah no rebanho /." Jaboticabal : [s.n.], 2008. http://hdl.handle.net/11449/92592.
Full textAbstract:
Resumo: Na expectativa de contribuir com os programas de melhoramento genético de bubalinos, o objetivo desse estudo foi verificar a influência dos efeitos ambientais e genéticos sobre a habilidade de permanência no rebanho de búfalas leiteiras da raça Murrah. Utilizaram-se informações de 1016 búfalas, participantes do programa de controle leiteiro mantido pelo Departamento de Zootecnia da UNESP/Jaboticabal(SP) desde 1987. A característica habilidade de permanência (HP) foi definida como a habilidade de permanecer no rebanho um (HP1), dois (HP2), três (HP3), quatro (HP4), cinco (HP5) e seis anos (HP6) após o primeiro parto. As mesmas características foram também consideradas como variável contínua, e denominadas Habilidade de Permanência em Dias até um (HPD1), dois (HPD2), três (HPD3), quatro (HPD4), cinco (HPD5) e seis anos (HPD6) após o primeiro parto. Os efeitos ambientais foram estudados por meio de análise de sobrevivência, ajustando-se os efeitos fixos de fazenda, ano e época de nascimento, classe de produção de leite na primeira lactação e idade ao primeiro parto. Fazenda, ano de nascimento e produção de leite na primeira lactação são efeitos que influenciam de forma significativa (p<0,0001) a permanência da fêmea no rebanho de 1 a 6 anos após o primeiro parto. Búfalas com maior idade ao primeiro parto têm maiores probabilidades de serem descartadas até um ano, sem efeito no descarte em idades mais avançadas. Búfalas com maior produção de leite têm menor probabilidade de descarte, permanecendo mais tempo no rebanho. Os efeitos genético-aditivo para as HP foram estimados por máxima verossimilhança restrita aproximada com modelo de limiar, enquanto que para as HPD foram estimados por máxima verossimilhança restrita...(Resumo completo, clicar acesso eletrônico abaixo)Abstract: In order of contributing to the buffalo genetic improvement programs , the objective of this study was to assess the influence of environmental and genetic effects on the stayability of the Murrah dairy buffaloes. Records from 1016 buffaloes, participants in the control dairy program maintained by the Departamento de Génetica e Melhoramento Animal of UNESP / Jaboticabal (SP) since 1987, were used. Stayability (HP) was defined as the ability to remain in the herd for one (HP1), two (HP2), three (HP3), four (HP4), five (HP5), and six years (HP6) after the first calving. The same traits were also considered continuous, and called as age of culling up to one (HPD1), two (HPD2), three (HPD3), four (HPD4), five (HPD5) and six years (HPD6) after the first calving. The environmental effects were studied by survival analysis. The model included the fixed effects of farm, year and season of birth, milk production class in the first lactation and age at first calving. The effects of farm, year of birth and milk production in the first lactation were significant (p <0.0001) for stayability from 1 to 6 years after the first calving.. Buffaloes cows with higher age at first calving are more likely to be culling a year after the first calve, without effect on the disposal at more advanced ages. Buffaloes with higher milk production are less likely to culling, staying longer in the herd. Additive genetic effects for HP were estimated by quasi-restricted maximum likelihood, fitting a threshold model, and the additive genetic effects for HPD were estimated by restricted maximum likelihood. The models included the additive genetic effects as random, ...(Complete abstract, click electronic access below)
Orientador: Humberto Tonhati
Coorientadora: Maria Eugênia Zerlotti Mercadante
Banca: Danísio Prado Munari
Banca: Lenira El Faro Zadra
Mestre
10
Caetano, Sabrina Luzia. "Estudo da idade da vaca ao último parto para avaliar longevidade em rebanhos da raça nelore por análise de sobrevivência /." Jaboticabal : [s.n.], 2011. http://hdl.handle.net/11449/102783.
Full textAbstract:
Resumo: No Brasil, existem poucos estudos sobre longevidade de vacas de corte, principalmente utilizando ferramentas de análise de sobrevivência na estimação de parâmetros genéticos. Todavia, os critérios para avaliar esta característica são vários, tal que em alguns destes nem todos os registros das vacas nos rebanhos podem ser levados em consideração, devido à metodologia de análise utilizada. Uma variável que é de fácil mensuração e já faz parte da maioria dos controles zootécnicos das fazendas é a idade da vaca ao último parto (IVUP). Neste trabalho, objetivo foi avaliar a longevidade das vacas nos rebanhos utilizando a variável IVUP, por meio da metodologia de análise de sobrevivência. Esta variável foi utilizada mediante um critério para analisar a longevidade produtiva de vacas nos rebanhos. O critério adotado foi a diferença entre a data em relação ao último parto de cada vaca e a data do último parto de cada fazenda. Se esta diferença foi superior a 36 meses, a vaca falhou e foi considerada descartada. Caso contrário, esta vaca foi censurada, indicando que esta ainda poderia ter futuras parições. O critério de 36 meses foi proposto por ser período suficiente para a ocorrência de um novo parto. A metodologia de análise de sobrevivência foi utilizada por considerar dados censurados e não censurados. As variáveis: estação e ano de nascimento de cada vaca, a fazenda e a idade ao primeiro parto foram utilizadas para as análises da variável IVUP. Um estudo prévio por meio de curvas de Kaplan-Meier e o modelo de Cox, utilizando a distribuição gama para os touros, desconsiderando o parentesco entre eles, foram realizados. O software Survival Kit foi empregado para estimação dos parâmetros genéticos, levando em consideração o parentesco entre os animais. Verificou-se que a IVUP apresentou herdabilidade de 0,25, e que seu uso permite avaliar a ... (Resumo completo, clicar acesso eletrônico abaixo)Abstract: In Brazil, there are few studies about stayability, especially using tools of survival analysis in the estimation of breeding values and heritability. The criteria for evaluating this characteristic are different, such that in some of these not all information in the herds of cows can be taken into consideration, because the method of analysis used. In addition, the definitions that consider the date of disposal of the animal affect the use of information obtained in practice, because until the cows come out of the flock may take years, thus the evaluation of their parents is impaired, since the goal is selection. A variable that is easy to measure and is already part of most controls husbandry farms are cow age at last birth (IVUP). This variable was used by one criterion to analyze the productive longevity of cows in herds. The criterion was the difference between the date from the last delivery date of each cow and the last part of each farm. If this difference was more than 36 months, the cow was considered failed and discarded. Otherwise, this cow was censored, indicating that this could still have further parities. The criterion of 36 months was proposed to be sufficient time for the occurrence of a new birth. The methodology of survival analysis was used. The variables season and year of birth of each cow, farm and age at first birth were used for analysis of variable IVUP. The objective of this study was to evaluate the performance of the variable IVUP through estimates of genetic and fixed effect, to study the longevity of cows in the herd. A previous study by Kaplan-Meier and Cox model using the gamma distribution for the bulls, disregarding the relationship between them, were performed. The Survival Kit software was used to estimate the genetic parameters, taking into account the relationship between the animals. It was found that the heritability of 0.25 for IVUP ... (Complete abstract click electronic access below)
Orientador: Danísio Prado Munari
Coorientador: Claudia Cristina Paro da Paz
Coorientador: Raysildo Barbosa Lobo
Banca: João Ademir de Oliveira
Banca: Henrique Nunes De Oliveira
Banca: Roberto Carvalheiro
Banca: Lenira El Faro Zadra
Doutor
Books on the topic "Survival. eng"
2
Karten, Naomi. Survival guide to end-user computing. Boston: Auerbach Publications, 1994.
Find full text
3
Barclay, Mark T. Preachers of righteousness: Critical to end times survival. Midland, MI: M. Barclay Publications, 1991.
Find full text
4
Glimmering light: The end begins : a novel. American Fork, Utah: Covenant Communications, Inc., 2014.
Find full text
6
Eekhout, Greg Van. The boy at the end of the world. New York: Bloomsbury Children's Books, 2011.
Find full text
7
Prescription for survival: A doctor's journey to end nuclear madness. San Francisco, Calif: Berrett-Koehler Publishers, 2008.
Find full text
8
H, Winters Ben, ed. The worst-case scenario survival handbook guide: Paranormal. San Francisco: Chronicle Books, 2011.
Find full text
9
How to survive the end of the world as we know it: Tactics, techniques, and technologies for uncertain times. New York, N.Y: Plume, 2009.
Find full text
10
Brunt, John. How to survive Armageddon. Hagerstown, MD: Review and Herald Pub. Association, 2011.
Find full textBook chapters on the topic "Survival. eng"
1
Isaacs, Bernard, Maureen Livingstone, and Yvonne Neville. "The East End of Glasgow." In Survival of the Unfittest, 4–7. London: Routledge, 2021. http://dx.doi.org/10.1201/9781003211570-3.
Full text
2
Japanwala, Natasha. "Adaptation, Migration, Advocacy. A Climate Change Curriculum for Out-of-School Children in Badin, Sindh." In Education and Climate Change, 137–52. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-57927-2_5.
Full textAbstract:
AbstractThis chapter identifies the urgency for climate change education in vulnerable communities that are already experiencing the effects of climate change-related disasters. Designing curricula for vulnerable communities, in this case out-of-school youth in Badin, a rural district in Pakistan’s Sindh province, demands a focus on strategies that can be leveraged for survival. This chapter illustrates the need to match curriculum design with research and reportage about the needs communities are facing. In Badin, where the local economy is driven by agriculture and threatened by the salinization of land as well as an increased risk to flooding, the possibility of migration is real. This shifts our understanding of what adaptation and mitigation mean for this population—youth need to be prepared not only to survive where they are, but to survive where they might end up.Vulnerable communities tend to reside in districts where the rates of literacy, school enrolment and retention are low—this is certainly the case in Badin. This curriculum had to rely on pedagogies for which literacy was not a pre-requisite. Project-based learning provided a unique solution to the conundrum of designing a no-literacy curriculum to teach strategies for survival in a community where best practices for adaptation, mitigation or migration have not yet been established: it allowed youth to work in teams, building their social and collaborative skills, to develop their own solutions and recognize the power of their own voice to advocate for their rights.
3
Heliövaara, K., R. Väisänen, and M. Varama. "Acidic Precipitation and Egg Survival in the European Pine Sawfly." In Responses of Forest Ecosystems to Environmental Changes, 963–64. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2866-7_244.
Full text
4
Bongiovanni, Francesco M. "Ensuring the Survival of a ‘Europe of Values’." In Europe and the End of the Age of Innocence, 191–203. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-74370-7_6.
Full text
5
Rowe, Victoria. "Armenian Women Refugees at the End of Empire: Strategies of Survival." In Refugees and the End of Empire, 152–72. London: Palgrave Macmillan UK, 2011. http://dx.doi.org/10.1057/9780230305700_7.
Full text
6
Nenner, Howard. "The Survival of Hereditary Monarchy and the End of Indefeasible Right." In The Right to be King, 147–83. London: Palgrave Macmillan UK, 1995. http://dx.doi.org/10.1007/978-1-349-12952-2_8.
Full text
7
van de Wiel, Ank. "6 How to survive?" In Engels voor zorg en welzijn, 89–102. Houten: Bohn Stafleu van Loghum, 2007. http://dx.doi.org/10.1007/978-90-313-9440-1_6.
Full text
8
Sanduleanu, Sebastian, Simon Keek, Lars Hoezen, and Philippe Lambin. "Biomarkers for Hypoxia, HPVness, and Proliferation from Imaging Perspective." In Critical Issues in Head and Neck Oncology, 13–20. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-63234-2_2.
Full textAbstract:
AbstractRecent advances in quantitative imaging with handcrafted radiomics and unsupervised deep learning have resulted in a plethora of validated imaging biomarkers in the field of head and neck oncology. Generally speaking, these algorithms are trained for one specific task, e.g. to classify between two or multiple types of underlying tumor biology (e.g. hypoxia, HPV status), predict overall survival (OS) or progression free survival (PFS), automatically segment a region of interest e.g. an organ at risk for radiotherapy dose or the gross tumor volume (GTV). Despite relatively good performances in external validation cohorts these algorithms still have not found their way into routine clinical practice. The reason this has not happened yet is complex, multifactorial, and can be usually divided into three categories: technical (a part of the algorithm or pre-processing step is not technically sound), statistical (mainly related to selection of subset of relevant biomarkers), and translational (not enough understanding by clinicians, not easily implementable within clinical workflow). We currently foresee that the next artificial intelligence (AI)-driven technique to find its way into clinical practice beside existing techniques (e.g. automatic organ at risk segmentation) will be the automatic segmentation of head and neck gross tumor volumes.
9
Van Glabbeke, M., and A. Bottomley. "Disease-free Survival and Quality of Life as End-points in Clinical Trials." In Surviving Intensive Care, 155–68. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-642-55733-0_13.
Full text
10
van de Wiel, Ank. "1 How to survive my career." In Engels voor zorg en welzijn, 13–27. Houten: Bohn Stafleu van Loghum, 2007. http://dx.doi.org/10.1007/978-90-313-9440-1_1.
Full textConference papers on the topic "Survival. eng"
1
Kapasi, Ali, Kieran Halloran, Alim Hirji, Dale Lien, Joshua Mooney, Rishi Raj, Andrew Sweatt, Justin Weinkauf, and Roham Zamanian. "Survival implications of pulmonary hypertension in end-stage COPD." In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.pa1426.
Full text
2
Occhipinti, Annalisa, and Claudio Angione. "A Computational Model of Cancer Metabolism for Personalised Medicine." In Building Bridges in Medical Science 2021. Cambridge Medicine Journal, 2021. http://dx.doi.org/10.7244/cmj.2021.03.001.3.
Full textAbstract:
Cancer cells must rewrite their ‘‘internal code’’ to satisfy the demand for growth and proliferation. Such changes are driven by a combination of genetic (e.g., genes’ mutations) and non-genetic factors (e.g., tumour microenvironment) that result in an alteration of cellular metabolism. For this reason, understanding the metabolic and genomic changes of a cancer cell can provide useful insight on cancer progression and survival outcomes. In our work, we present a computational framework that uses patient-specific data to investigate cancer metabolism and provide personalised survival predictions and cancer development outcomes. The proposed model integrates patient-specific multi-omics data (i.e., genomic, metabolomic and clinical data) into a metabolic model of cancer to produce a list of metabolic reactions affecting cancer progression. Quantitative and predictive analysis, through survival analysis and machine learning techniques, is then performed on the list of selected reactions. Since our model performs an analysis of patient-specific data, the outcome of our pipeline provides a personalised prediction of survival outcome and cancer development based on a subset of identified multi-omics features (genomic, metabolomic and clinical data). In particular, our work aims to develop a computational pipeline for clinicians that relates the omic profile of each patient to their survival probability, based on a combination of machine learning and metabolic modelling techniques. The model provides patient-specific predictions on cancer development and survival outcomes towards the development of personalised medicine.
3
Weaver, John, Tae Jin Kang, Kimberly Raines, Guan-Liang Cao, Stephen Hibbs, Pei Tsai, Les Baillie, Gerald Rosen, and Alan Cross. "The Protective Role of Bacillus Anthracis Exosporium in Macrophage-Mediated Killing by Nitric Oxide." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176138.
Full textAbstract:
The ability of the endospore-forming, gram-positive bacterium Bacillus anthracis to survive exposure to antibacterial killing mechanisms by activated macrophages is key to its germination and survival. These antibacterial killing mechanisms include, but are not limited to the generation of free radicals such as nitric oxide (•NO) and superoxide (O2•−) from the upregulation of inducible nitric oxide synthase (NOS 2) along with products derived from them, e.g., peroxynitrite (ONOO−), as part of microbicidal activity. However questions still remain as to how these species are involved in microbial killing, specifically with respect to B. anthracis. In a previous study, we demonstrated that exposure of primary murine macrophages to sonicated B. anthracis endospores up-regulated NOS 2 and demonstrated a •NO-dependent bactericidal response, but unanswered in that study was which of the NOS 2-derived reactive oxygen species was responsible for the observed bactericidal response. Since NOS 2 also generates O2•−, experiments were designed to determine whether NOS 2 formed ONOO− from the reaction of •NO with O2•− and if so, was ONOO− microbicidal toward B. anthracis.
4
Moskvina, Anastasiia, and Iuliia Pravnik. "VALUE GUIDELINES IN THE ANGLE OF THE PROBLEM OF HUMANITY SURVIVAL." In Perspectives et mise en oeuvre de l'innovation dans le domaine scientifique. Plateforme scientifique européenne, 2019. http://dx.doi.org/10.36074/20.09.2019.v1.02.
Full text
5
Dabideen, Stephen, Bradley R. Smith, and J. J. Garcia-Luna-Aceves. "An end-to-end solution for secure and survivable routing in MANETs." In 2009 7th International Workshop on Design of Reliable Communication Networks (DRCN). IEEE, 2009. http://dx.doi.org/10.1109/drcn.2009.5340007.
Full text
6
Acaccia, Gabriella M., Rinaldo C. Michelini, Luca Penzo, and Nicola Qualich. "Automotive Systems: End-of-Life Vehicles Dismantling Facilities." In ASME 8th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2006. http://dx.doi.org/10.1115/esda2006-95332.
Full textAbstract:
The paper discusses characterising features of the end-of-life vehicles EU regulation, with account of the process transparency and knowledge build-up, to tailor economic instruments with reverse logistics profitability. In the short future, these issues will affect the automotive market, and the recovery (reuse/recycle) targets will become competition prerequisites for the carmakers survival. For explanatory purposes, the investigation is especially limited to consider the dismantling facility, with, in foreground, facts and technicalities arising at local and regional scales, and, in background, the whole forward and backward supply chain and related logistics problems. The basic technicalities, leading to the build-up of the reference knowledge for the most effective setting of car-dismantling facilities, are explored by means of simulation results. These lead to consider to develop automotive systems with recovery in mind, according to the carmakers responsibility for the free-take-back of the end-of-life vehicles.
7
Bernstein, Adam J., Alison L. Marsden, Ryan L. Spilker, V. Mohan Reddy, Charles A. Taylor, and Jeffrey A. Feinstein. "Evaluation of Hemodynamic Efficiency in a New “Y-Graft” Design for the Fontan Operation." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176279.
Full textAbstract:
Hypoplastic left heart syndrome is a congenital heart defect that occurs in 20 per 100,000 live births. Patients are born with severe underdevelopment of the left side of the heart which, if left untreated, is uniformly fatal. A series of operations is performed, including a cavopulmonary (Glenn) shunt and total cavopulmonary connection (Fontan procedure), which connect the superior (SVC) and inferior vena cavae (IVC) respectively in an end-to-side fashion to the left (LPA) and right pulmonary arteries (RPA), resulting in a T-shaped junction. This bypasses the heart on the venous side as blood flows from the IVC and SVC directly into the pulmonary arteries. Early survival rates following the Fontan are as high as 90%. However, these figures drop to 60% survival after 10 years [1], and most patients exhibit diminished exercise capacity.
8
Phadke, Amal C., Shihwei Liao, and Donghui Chen. "Assessment of SYMS Survival Limit for “Peng Bo” FPSO During Installation." In ASME 2010 29th International Conference on Ocean, Offshore and Arctic Engineering. ASMEDC, 2010. http://dx.doi.org/10.1115/omae2010-20817.
Full textAbstract:
The ConocoPhillips “Peng Bo” FPSO in Bohai Bay, China, is one of the largest FPSOs in the world. It was installed in early 2009 and first oil was achieved in May 2009. The FPSO is moored to a jacket platform in about 27m water depth via a Soft Yoke Mooring System (SYMS). The mooring system consists of two yoke arms (port and starboard) connected at the jacket end to a turn-table that sits on a main bearing at the top of the jacket. The other ends of the yoke arms are integrated with two large ballast tanks which are attached to two pendulums supported by a rigid frame mounted on the forward end of the FPSO. The ballast weights give rise to the necessary system surge restoring force while the turntable and bearing allow the FPSO to weathervane. The yoke arms and ballast tanks are pre-installed with empty ballast tanks mounted on two temporary support barges while awaiting the arrival of the FPSO. Upon arrival, the ballast tanks are attached to the pendulums from the FPSO side. The tanks are then filled with solid concrete ballast to achieve the design weight. Before the tanks are completely filled, the SYMS may not have sufficient restoring force in partially filled condition to prevent excessive pitching of the yoke arms and severe bearing load on the jacket beyond the design limit due to large FPSO offset in the event of an unforeseen storm event. An analysis was carried out to estimate the maximum bearing load and yoke arm pitch angle when the ballast tanks were 0%, 25%, 50%, and 75% full for 10-year and 100-year return period events. This paper describes the analysis methodology and results from the analysis. The overall project and installation experience are also discussed.
9
S. Pahl, Eric, W. Nick Street, Hans J. Johnson, and Alan I. Reed. "A Predictive Model for Kidney Transplant Graft Survival using Machine Learning." In 4th International Conference on Computer Science and Information Technology (COMIT 2020). AIRCC Publishing Corporation, 2020. http://dx.doi.org/10.5121/csit.2020.101609.
Full textAbstract:
Kidney transplantation is the best treatment for end-stage renal failure patients. The predominant method used for kidney quality assessment is the Cox regression-based, kidney donor risk index. A machine learning method may provide improved prediction of transplant outcomes and help decision-making. A popular tree-based machine learning method, random forest, was trained and evaluated with the same data originally used to develop the risk index (70,242 observations from 1995-2005). The random forest successfully predicted an additional 2,148 transplants than the risk index with equal type II error rates of 10%. Predicted results were analyzed with follow-up survival outcomes up to 240 months after transplant using Kaplan-Meier analysis and confirmed that the random forest performed significantly better than the risk index (p<0.05). The random forest predicted significantly more successful and longer-surviving transplants than the risk index. Random forests and other machine learning models may improve transplant decisions.
10
Pappafotis, Nicholas, Wojciech Bejgerowski, Rao Gullapalli, J. Marc Simard, Satyandra K. Gupta, and Jaydev P. Desai. "Towards Design and Fabrication of a Miniature MRI-Compatible Robot for Applications in Neurosurgery." In ASME 2008 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2008. http://dx.doi.org/10.1115/detc2008-49587.
Full textAbstract:
Brain tumors are among the most feared complications of cancer and they occur in 20–40% of adult cancer patients. Despite numerous advances in treatment, the prognosis for these patients is poor, with a median survival of 4–8 months. The primary reasons for poor survival rate are the lack of good continuous imaging modality for intraoperative intracranial procedures and the inability to remove the complete tumor tissue due to its placement in the brain and the corresponding space constraints to reach it. Intraoperative magnetic resonance imaging (MRI) supplements the surgeon’s visual and tactile senses in a way that no other imaging device can achieve resulting in less trauma to surrounding healthy brain tissue during surgery. To minimize the trauma to surrounding healthy brain tissue, it would be beneficial to operate through a narrow surgical corridor dissected by the neurosurgeon. Facilitating tumor removal by accessing regions outside the direct “line-of-sight” of the neurosurgical corridor will require a highly dexterous, small cross section, and MRI-compatible robot. Developing such a robot is extremely challenging task. In this paper we report a preliminary design of 6-DOF robot for possible application in neurosurgery. The robot actuators and body parts are constructed from MRI compatible materials. The current prototype is 0.36” in diameter and weighs only 0.0289 N (2.95 grams). The device was actuated using Flexinol® which is a shape memory alloy manufactured by Dynalloy, Inc. The end-effector forces ranged from 12 mN to 50 mN depending on the robot configuration. The end-effector force to robot weight ratio varied from 0.41 to 1.73. During trials the robot motion was repeatable and the range of motion of the robot was about 90 degrees for the end-effector when one side shape memory alloy (SMA) channel was actuated. The actuation time from the start to finish was about 2.5 s.
Reports on the topic "Survival. eng"
1
Warner, Daniel, Timothy Mitchell, and Fredric J. Janzen. The Effects of Egg-incubation Temperature on Growth and Survival of Hatchling Painted Turtles. Ames: Iowa State University, Digital Repository, 2011. http://dx.doi.org/10.31274/farmprogressreports-180814-505.
Full text
2
Idris, Iffat. Documentation of Survivors of Gender-based Violence (GBV). Institute of Development Studies (IDS), July 2021. http://dx.doi.org/10.19088/k4d.2021.103.
Full textAbstract:
This review is largely based on grey literature, in particular policy documents and reports by international development organizations. While there was substantial literature on approaches and principles to GBV documentation, there was less on remote service delivery such as helplines – much of this only in the wake of the COVID-19 pandemic. In addition, very little was found on actual examples of GBV documentation in developing contexts. By definition, gender featured strongly in the available literature; the particular needs of persons with disabilities were also addressed in discussions of overall GBV responses, but far less in GBV documentation. GBV documentation refers to the recording of data on individual GBV incidents in order to provide/refer survivors with/to appropriate support, and the collection of data of GBV incidents for analysis and to improve GBV responses. The literature notes that there are significant risks associated with GBV documentation, in relation to data protection. Failure to ensure information security can expose survivors, in particular, to harm, e.g. reprisal attacks by perpetrators, stigma, and ostracism by their families/ communities. This means that GBV documentation must be carried out with great care. A number of principles should always be applied when documenting GBV cases in order to protect survivors and prevent potential negative effects: do no harm, survivor-centered approach, survivor autonomy, informed consent, non-discrimination, confidentiality, and data protection (information security).
3
Mobley, Erin M., Diana J. Moke, Joel Milam, Carol Y. Ochoa, Julia Stal, Nosa Osazuwa, Maria Bolshakova, et al. Disparities and Barriers to Pediatric Cancer Survivorship Care. Agency for Healthcare Research and Quality (AHRQ), March 2021. http://dx.doi.org/10.23970/ahrqepctb39.
Full textAbstract:
Objectives. Survival rates for pediatric cancer have dramatically increased since the 1970s, and the population of childhood cancer survivors (CCS) exceeds 500,000 in the United States. Cancer during childhood and related treatments lead to long-term health problems, many of which are poorly understood. These problems can be amplified by suboptimal survivorship care. This report provides an overview of the existing evidence and forthcoming research relevant to disparities and barriers for pediatric cancer survivorship care, outlines pending questions, and offers guidance for future research. Data sources. This Technical Brief reviews published peer-reviewed literature, grey literature, and Key Informant interviews to answer five Guiding Questions regarding disparities in the care of pediatric survivors, barriers to cancer survivorship care, proposed strategies, evaluated interventions, and future directions. Review methods. We searched research databases, research registries, and published reviews for ongoing and published studies in CCS to October 2020. We used the authors’ definition of CCS; where not specified, CCS included those diagnosed with any cancer prior to age 21. The grey literature search included relevant professional and nonprofit organizational websites and guideline clearinghouses. Key Informants provided content expertise regarding published and ongoing research, and recommended approaches to fill identified gaps. Results. In total, 110 studies met inclusion criteria. We identified 26 studies that assessed disparities in survivorship care for CCS. Key Informants discussed subgroups of CCS by race or ethnicity, sex, socioeconomic status, and insurance coverage that may experience disparities in survivorship care, and these were supported in the published literature. Key Informants indicated that major barriers to care are providers (e.g., insufficient knowledge), the health system (e.g., availability of services), and payers (e.g., network adequacy); we identified 47 studies that assessed a large range of barriers to survivorship care. Sixteen organizations have outlined strategies to address pediatric survivorship care. Our searches identified only 27 published studies that evaluated interventions to alleviate disparities and reduce barriers to care. These predominantly assessed approaches that targeted patients. We found only eight ongoing studies that evaluated strategies to address disparities and barriers. Conclusions. While research has addressed disparities and barriers to survivorship care for childhood cancer survivors, evidence-based interventions to address these disparities and barriers to care are sparse. Additional research is also needed to examine less frequently studied disparities and barriers and to evaluate ameliorative strategies in order to improve the survivorship care for CCS.
4
Hutchinson, M. L., J. E. L. Corry, and R. H. Madden. A review of the impact of food processing on antimicrobial-resistant bacteria in secondary processed meats and meat products. Food Standards Agency, October 2020. http://dx.doi.org/10.46756/sci.fsa.bxn990.
Full textAbstract:
For meat and meat products, secondary processes are those that relate to the downstream of the primary chilling of carcasses. Secondary processes include maturation chilling, deboning, portioning, mincing and other operations such as thermal processing (cooking) that create fresh meat, meat preparations and ready-to-eat meat products. This review systematically identified and summarised information relating to antimicrobial resistance (AMR) during the manufacture of secondary processed meatand meat products (SPMMP). Systematic searching of eight literature databases was undertaken and the resultantpapers were appraised for relevance to AMR and SPMMP. Consideration was made that the appraisal scores, undertaken by different reviewers, were consistent. Appraisal reduced the 11,000 initially identified documents to 74, which indicated that literature relating to AMR and SPMMP was not plentiful. A wide range of laboratory methods and breakpoint values (i.e. the concentration of antimicrobial used to assess sensitivity, tolerance or resistance) were used for the isolation of AMR bacteria.The identified papers provided evidence that AMR bacteria could be routinely isolated from SPMMP. There was no evidence that either confirmed or refuted that genetic materials capable of increasing AMR in non-AMR bacteria were present unprotected (i.e. outside of a cell or a capsid) in SPMMP. Statistical analyses were not straightforward because different authors used different laboratory methodologies.However, analyses using antibiotic organised into broadly-related groups indicated that Enterobacteriaceaeresistant to third generation cephalosporins might be an area of upcoming concern in SPMMP. The effective treatment of patients infected with Enterobacteriaceaeresistant to cephalosporins are a known clinical issue. No AMR associations with geography were observed and most of the publications identified tended to be from Europe and the far east.AMR Listeria monocytogenes and lactic acid bacteria could be tolerant to cleaning and disinfection in secondary processing environments. The basis of the tolerance could be genetic (e.g. efflux pumps) or environmental (e.g. biofilm growth). Persistent, plant resident, AMR L. monocytogenes were shown by one study to be the source of final product contamination. 4 AMR genes can be present in bacterial cultures used for the manufacture of fermented SPMMP. Furthermore, there was broad evidence that AMR loci could be transferred during meat fermentation, with refrigeration temperatures curtailing transfer rates. Given the potential for AMR transfer, it may be prudent to advise food business operators (FBOs) to use fermentation starter cultures that are AMR-free or not contained within easily mobilisable genetic elements. Thermal processing was seen to be the only secondary processing stage that served as a critical control point for numbers of AMR bacteria. There were significant linkages between some AMR genes in Salmonella. Quaternary ammonium compound (QAC) resistance genes were associated with copper, tetracycline and sulphonamide resistance by virtue of co-location on the same plasmid. No evidence was found that either supported or refuted that there was any association between AMR genes and genes that encoded an altered stress response or enhanced the survival of AMR bacteria exposed to harmful environmental conditions.
5
Tymoshyk, Mykola. LONDON MAGAZINE «LIBERATION WAY» AND ITS PLACE IN THE HISTORY OF UKRAINIAN JOURNALISM ABROAD. Ivan Franko National University of Lviv, February 2021. http://dx.doi.org/10.30970/vjo.2021.49.11057.
Full textAbstract:
One of the leading Western Ukrainian diaspora journals – London «Liberation Way», founded in January 1949, has become the subject of the study for the first time in journalism. Archival documents and materials of the Ukrainian Publishing Union in London and the British National Library (British Library) were also observed. The peculiarities of the magazine’s formation and the specifics of the editorial policy, founders and publishers are clarified. A group of OUN members who survived Hitler’s concentration camps and ended up in Great Britain after the end of World War II initiated the foundation of the magazine. Until April 1951, including issue 42, the Board of Foreign Parts of the Organization of Ukrainian Nationalists were the publishers of the magazine. From 1951 to the beginning of 2000 it was a socio-political monthly of the Ukrainian Publishing Union. From the mid-60’s of the twentieth century – a socio-political and scientific-literary monthly. In analyzing the programmatic principles of the magazine, the most acute issues of the Ukrainian national liberation movement, which have long separated the forces of Ukrainian emigration and from which the founders and publishers of the magazine from the beginning had clearly defined positions, namely: ideology of Ukrainian nationalism, the idea of unity of Ukraine and Ukrainians, internal inter-party struggle among Ukrainian emigrants have been singled out. The review and systematization of the thematic palette of the magazine’s publications makes it possible to distinguish the following main semantic accents: the formation of the nationalist movement in exile; historical Ukrainian themes; the situation in sub-Soviet Ukraine; the problem of the unity of Ukrainians in the Western diaspora; mission and tasks of Ukrainian emigration in the context of its responsibilities to the Motherland. It also particularizes the peculiarities of the formation of the author’s assets of the magazine and its place in the history of Ukrainian national journalism.
6
Saville, Alan, and Caroline Wickham-Jones, eds. Palaeolithic and Mesolithic Scotland : Scottish Archaeological Research Framework Panel Report. Society for Antiquaries of Scotland, June 2012. http://dx.doi.org/10.9750/scarf.06.2012.163.
Full textAbstract:
Why research Palaeolithic and Mesolithic Scotland? Palaeolithic and Mesolithic archaeology sheds light on the first colonisation and subsequent early inhabitation of Scotland. It is a growing and exciting field where increasing Scottish evidence has been given wider significance in the context of European prehistory. It extends over a long period, which saw great changes, including substantial environmental transformations, and the impact of, and societal response to, climate change. The period as a whole provides the foundation for the human occupation of Scotland and is crucial for understanding prehistoric society, both for Scotland and across North-West Europe. Within the Palaeolithic and Mesolithic periods there are considerable opportunities for pioneering research. Individual projects can still have a substantial impact and there remain opportunities for pioneering discoveries including cemeteries, domestic and other structures, stratified sites, and for exploring the huge evidential potential of water-logged and underwater sites. Palaeolithic and Mesolithic archaeology also stimulates and draws upon exciting multi-disciplinary collaborations. Panel Task and Remit The panel remit was to review critically the current state of knowledge and consider promising areas of future research into the earliest prehistory of Scotland. This was undertaken with a view to improved understanding of all aspects of the colonization and inhabitation of the country by peoples practising a wholly hunter-fisher-gatherer way of life prior to the advent of farming. In so doing, it was recognised as particularly important that both environmental data (including vegetation, fauna, sea level, and landscape work) and cultural change during this period be evaluated. The resultant report, outlines the different areas of research in which archaeologists interested in early prehistory work, and highlights the research topics to which they aspire. The report is structured by theme: history of investigation; reconstruction of the environment; the nature of the archaeological record; methodologies for recreating the past; and finally, the lifestyles of past people – the latter representing both a statement of current knowledge and the ultimate aim for archaeologists; the goal of all the former sections. The document is reinforced by material on-line which provides further detail and resources. The Palaeolithic and Mesolithic panel report of ScARF is intended as a resource to be utilised, built upon, and kept updated, hopefully by those it has helped inspire and inform as well as those who follow in their footsteps. Future Research The main recommendations of the panel report can be summarized under four key headings: Visibility: Due to the considerable length of time over which sites were formed, and the predominant mobility of the population, early prehistoric remains are to be found right across the landscape, although they often survive as ephemeral traces and in low densities. Therefore, all archaeological work should take into account the expectation of Palaeolithic and Mesolithic ScARF Panel Report iv encountering early prehistoric remains. This applies equally to both commercial and research archaeology, and to amateur activity which often makes the initial discovery. This should not be seen as an obstacle, but as a benefit, and not finding such remains should be cause for question. There is no doubt that important evidence of these periods remains unrecognised in private, public, and commercial collections and there is a strong need for backlog evaluation, proper curation and analysis. The inadequate representation of Palaeolithic and Mesolithic information in existing national and local databases must be addressed. Collaboration: Multi-disciplinary, collaborative, and cross- sector approaches must be encouraged – site prospection, prediction, recognition, and contextualisation are key areas to this end. Reconstructing past environments and their chronological frameworks, and exploring submerged and buried landscapes offer existing examples of fruitful, cross-disciplinary work. Palaeolithic and Mesolithic archaeology has an important place within Quaternary science and the potential for deeply buried remains means that geoarchaeology should have a prominent role. Innovation: Research-led projects are currently making a substantial impact across all aspects of Palaeolithic and Mesolithic archaeology; a funding policy that acknowledges risk and promotes the innovation that these periods demand should be encouraged. The exploration of lesser known areas, work on different types of site, new approaches to artefacts, and the application of novel methodologies should all be promoted when engaging with the challenges of early prehistory. Tackling the ‘big questions’: Archaeologists should engage with the big questions of earliest prehistory in Scotland, including the colonisation of new land, how lifestyles in past societies were organized, the effects of and the responses to environmental change, and the transitions to new modes of life. This should be done through a holistic view of the available data, encompassing all the complexities of interpretation and developing competing and testable models. Scottish data can be used to address many of the currently topical research topics in archaeology, and will provide a springboard to a better understanding of early prehistoric life in Scotland and beyond.