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Dissertations / Theses on the topic 'Suspension cells'

Author: Grafiati

Published: 4 June 2021

Last updated: 3 February 2022

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1

Bayer, Emmanuelle M. "Plasmodesmata in Arabidopsis suspension cells." Thesis, University of East Anglia, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.423814.

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2

Aunins, John Grant. "Induced flocculation of animal cells in suspension culture." Thesis, Massachusetts Institute of Technology, 1989. http://hdl.handle.net/1721.1/14330.

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3

Walther, Christa G. "Adaptation to suspension growth : analysing the surface of suspension growth adapted Chinese hamster ovary cells." Thesis, University of Sheffield, 2013. http://etheses.whiterose.ac.uk/4193/.

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Many bio-pharmaceutical production processes are based upon the use of mammalian cell lines, such as Chinese hamster ovary (CHO) cells, capable of proliferation as single cells in suspension in a synthetic environment. Routine use of CHO cells as production vehicles requires a lengthy “adaptation” process from the wild-type adherent clone to clones capable of proliferation in a suspension environment depleted of exogenous growth factors and cell-matrix contacts. Different approaches have been applied in this study to gain a better understanding of the changes on the cell surface occurring as a

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4

Lagares, Lemos Miguel. "Matlab Based Specific Impedance Spectroscopy Simulator for Suspension of Cells." Thesis, Högskolan i Borås, Institutionen Ingenjörshögskolan, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19348.

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By means of the analytical formula of specific impedance for the spherical cells in suspensionintroduced by Kenneth S. Cole in 1928, a bioimpedance simulator has been developed for thegeneration of specific impedance spectrums of suspension of cells. With the help of the simulatorthe user can obtain different impedance spectrums according with the biophysical parameters ofthe cell suspension. Then, generate different kind of plots in order to understand and interpret allthe resulting information.With the selection of different values and range of the biophysical parameters to obtain thespectru

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5

Thompson, James. "Xyloglucan metabolism in the apoplast of suspension-cultured rose cells." Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/13110.

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Xyloglucan hydrogen-bonds to cellulose and has been proposed to form load-bearing cross-links between adjacent microfibrils in the primary cell walls of plant cells. Such cross-links may regulate wall extensibility and allow control of cell expansion. Changes in relative molecular mass (M<SUB>r</SUB>) of xyloglucan have been correlated with wall extensibility in several studies. Endotransglycosylation of xyloglucan may cut and reform cross-links, enabling limited cell expansion through temporary loosening of the cell wall. An enzyme capable of catalysing such a reaction, xyloglucan endotransgl

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6

Ketchum, Karen Ann. "A calcium-dependent potassium channel in corn (Zea mays) suspension cells /." Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=74658.

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Three distinct K$ sp+$ currents were identified in corn (Zea mays) protoplasts using the whole-cell patch-clamp technique. Inward-rectifying K$ sp+$ currents were evoked at membrane potentials more negative than $-$100 mV. The activation range was sensitive to external K$ sp+$ and shifted in the positive direction as the K$ sp+$ concentration was elevated. The second K$ sp+$ current was voltage-independent and contributed to the resting membrane conductance of the protoplast. Finally, a voltage- and Ca$ sp{2+}$-dependent K$ sp+$ current was observed at potentials positive to $-$60 mV. This cur

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7

Jaini, Rajiv. "Mass-transfer correlations for the dual bed colloidal suspension reactor." Thesis, Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/50210.

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To meet the growing energy world demands, and in conjunction, lower CO2 production levels, near zero emission energy sources must be pushed to the forefront as alternatives to fossil fuels. Photoelectrochemical (PEC) cells are a potential alternative to fossil fuels and have recently generated much interest because of their potential to electrolyze water into hydrogen fuel from sunlight. But in order to be competitive with fossil fuels, understanding the mass-transfer limitations in PEC systems is critical. This work focuses on the addressing the mass-transfer limitations in a conceptually

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8

Cabeça, Tatiane Karen [UNESP]. "Suscetibilidade de microorganismos relacionados com a contaminação de alimentos em biofilme artificial e em suspensão frente a desinfetantes." Universidade Estadual Paulista (UNESP), 2006. http://hdl.handle.net/11449/94813.

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Made available in DSpace on 2014-06-11T19:27:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-08-10Bitstream added on 2014-06-13T20:16:30Z : No. of bitstreams: 1 cabeca_tk_me_arafcf.pdf: 1210665 bytes, checksum: 4362d616ef5872f20b6711858bce8e88 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Avaliou-se o perfil de suscetibilidade de Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922 e Listeria monocytogenes ATCC 7644, em suspensão e em biofilme formado in vitro, frente à ação dos desinfetantes: ácido iodado, à base de biguanida, à base de qu

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9

Kang, Jane. "Migration of blood cells in non-uniform suspension for a dialyzer design." Diss., Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/53871.

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Hemodialysis is a renal replacement therapy that removes waste solutes from the blood stream using concentration gradients across a membrane. In order to overcome several shortcomings and increase the waste removal rate, a new dialyzer (filter) design is proposed in this study. In the new dialyzer design, the blood concurrently flows with a sheath fluid in a micro-fluidic channel. Because the blood stream directly contacts the sheath stream, it is important to prevent blood cell migration from the blood stream to the sheath stream while providing enough time for the waste solutes to diffuse

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10

Smith, Christina Kimmer. "Naturally occurring oligosaccharin secreted by rose cells into their suspension culture medium." Thesis, University of Edinburgh, 1998. http://hdl.handle.net/1842/11414.

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I analysed spent culture medium from plant cell suspension cultures for the presence of naturally occurring oligosaccharides. A potential oligosaccharin was purified from the spent culture medium of rose (<I>Rosa </I>sp.) cells. The oligosaccharide was purified by anion exchange chromatography, gel permeation chromatography and HPLC. Its structure was determined by paper chromatography, HPLC and NMR and found to be a trisaccharide: α-D-mannopyranosyl-(1→4)-α-D-glucuronopyranosyl-(1→2)-<I>myo</I>-inositol (Man-GlcA-Ins). It accumulated in the culture medium at a steady rate between 6 and 19 day

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11

Sá, Mário Moniz de. "Induction of phenylpropanoid metabolism in elicitor-treated hybrid poplar suspension-cultured cells." Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/30296.

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Induction of phenylpropanoid metabolism in many plants is associated with the induction of plant defence responses. Among these are the accumulation of phenylpropanoid-derived phytoalexins, increase in lignification around infected sites, and the accumulation of wall-bound phenolic compounds. I show in this work, that H11 hybrid cell suspension cultures when treated with either of three elicitors respond with an increase in phenylpropanoid metabolism. Activation proceeds rapidly from PAL and 4CL mRNA accumulation, to a massive increase in extractable PAL enzyme activity and finally there is ac

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12

Vasquez, de Beeching Rocio Gomez. "Plant defence responses in cassava (Manihot esculenta Crantz) suspension cells challenged with elicitors." Thesis, University of Bath, 2000. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.760749.

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13

Case, Natasha D. "Oscillatory Compressive Loading Effects On Mesenchymal Progenitor Cells Undergoing Chondrogenic Differentiation In Hydrogel Suspension." Diss., Georgia Institute of Technology, 2005. http://hdl.handle.net/1853/6939.

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Articular cartilage functions to maintain joint mobility. The loss of healthy, functional articular cartilage due to osteoarthritis or injury can severely compromise quality of life. To address this issue, cartilage tissue engineering approaches are currently in development. Bone marrow-derived mesenchymal progenitor cells (MPCs) hold much promise as an alternative cell source for cartilage tissue engineering. While previous studies have established that MPCs from humans and multiple other species undergo in vitro chondrogenic differentiation, additional research is needed to define condit

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14

Cabeça, Tatiane Karen. "Suscetibilidade de microorganismos relacionados com a contaminação de alimentos em biofilme artificial e em suspensão frente a desinfetantes /." Araraquara : [s.n.], 2006. http://hdl.handle.net/11449/94813.

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Orientador: Elisabeth Loshchagin Pizzolitto<br>Banca: Jonas Contiero<br>Banca: Cristina Paiva de Sousa<br>Resumo: Avaliou-se o perfil de suscetibilidade de Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922 e Listeria monocytogenes ATCC 7644, em suspensão e em biofilme formado in vitro, frente à ação dos desinfetantes: ácido iodado, à base de biguanida, à base de quaternário de amônio, à base de ácido peracético e clorado, empregados comumente em indústrias alimentícias brasileiras. As técnicas de microscopia eletrônica de varredura e microbiológica de viabilidade celular foram utili

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15

Smith, Christopher R. "Purification and characterization of cytosolic and leucoplast pyruvate kinase isoenzymes from Brassica napus suspension cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0021/MQ54485.pdf.

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16

Nöldner, Pamela Kim-Mai. "The role of phospholipases in signalling an elicitor-induced oxidative burst in tomato suspension cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0002/MQ40700.pdf.

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17

Montgomery, Sarah Lynn. "Impedance measurement system for embryonic stem cell and embryoid body cultures." Thesis, Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/24661.

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18

Benitex, Yulianingsih. "The effects of phenetyl isothiocyanate and benzyl isothiocyanate on acetaminophen metabolism and toxicity in freshly isolated rat hepatocytes in cell suspension /." View abstract, 1999. http://library.ctstateu.edu/ccsu%5Ftheses/1563.html.

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Thesis (M.S.)--Central Connecticut State University, 1999.<br>Thesis advisor: Carol A. Jones. " ... in partial fulfillment of the requirements for the degree of Master of Science in Chemistry." Includes bibliographical references (leaves 42-51).

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19

Elshereef, Abdalla Adel Sabry Abdelrazik Mohamed [Verfasser]. "Optimization of transient protein production by chemically transfected CHO suspension cells / Abdalla Adel Sabry Abdelrazik Mohamed Elshereef." Hannover : Technische Informationsbibliothek (TIB), 2018. http://d-nb.info/1166088472/34.

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20

Elshereef, Abdalla [Verfasser]. "Optimization of transient protein production by chemically transfected CHO suspension cells / Abdalla Adel Sabry Abdelrazik Mohamed Elshereef." Hannover : Technische Informationsbibliothek (TIB), 2018. http://d-nb.info/1166088472/34.

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21

Peschel, Britta [Verfasser]. "Infection dynamics and virus-induced apoptosis in influenza virus A infected adherent and suspension MDCK cells / Britta Peschel." Aachen : Shaker, 2015. http://d-nb.info/1074087348/34.

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22

Provchy, Kristan. "Von Willebrand Factor Expression in Vascular Endothelial Cells of Cage Control and Antiorthostatic Cage Suspension Golden Hamster Ovaries." Digital Commons @ East Tennessee State University, 2010. https://dc.etsu.edu/honors/7.

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The hamster estrous cycle lasts four days and is considered to be a physiological model for angiogenesis. Angiogenesis is the formation of new capillaries from preexisting vessels, and it occurs extensively during corpus luteum formation in the estrous cycle. Von Willebrand Factor (vWF) is a glycoprotein that is secreted uniquely in endothelial cells and megakaryocytes. It is frequently used as an endothelial cell marker and it is able to detect vessels within tissues when it is used in immunohistochemical staining techniques. This study explores von Willebrand Factor expression within Gol

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23

McLaren, John. "Human renal proximal tubular cells, in suspension and primary culture, as in vitro models of drug-induced nephrotoxicity." Thesis, University of Aberdeen, 1992. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU545620.

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The kidney is the target for a wide variety of chemical agents, including heavy metals, haloalkenes, analgesics and antibiotics. The functional and metabolic characteristics of the proximal tubule (PT) predispose it as the primary site for xenobiotic damage. The aim of this study was to isolate and characterise human and rat PT cells in suspension and primary culture for use as defined models to investigate drug-induced PT cell damage in vitro . A second aim was to compare the response of human and rat systems to known nephrotoxins. Human and rat PT cells (90&'37 viable) were isolated from kid

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24

Ullisch, David Alexander [Verfasser]. "A fundamental research of growth, metabolism and product formation of tobacco suspension cells at different scales / David Alexander Ullisch." Aachen : Hochschulbibliothek der Rheinisch-Westfälischen Technischen Hochschule Aachen, 2012. http://d-nb.info/1027766668/34.

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25

Allabidi, Abdulrahman Abdalla. "COMPARISON BETWEEN FLOW CYTOMETRY AND BEAD METHOD IN COUNTING CD4 AND CD8 T LYMPHOCYTES IN MOUSE SPLEEN CELLS SUSPENSION." Wright State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=wright1421242306.

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26

Terrasso, Ana Paula Barreto. "Development of novel human cellular models for neurotoxicity studies." Master's thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/8488.

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Dissertation to obtain master degree in Genética Molecular e Biomedicina<br>Information currently available on neurotoxicity of chemicals is scarce and there are a growing number of new compounds to be tested. Therefore, new strategies are necessary to identify neurotoxic agents with speed, reliability and respect for animal welfare. The limited availability of primary human brain cells means that there is a need for human cell lines that reliably model human neurons and astrocytes. Despite the advances in stem cell research, numerous challenges must be overcome before this technology can be

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27

Rottmayer, Regine. "A short-term suspension culture system for bovine oviduct epithelial cells suitable for the study of embryo-maternal communication processes." Diss., [S.l.] : [s.n.], 2006. http://edoc.ub.uni-muenchen.de/archive/00005603.

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28

Nivelle, Laetitia. "Etude des propriétés anti-tumorales in vitro de stilbènes issus de suspension cellulaire de vigne (Vitis labrusca)." Thesis, Reims, 2017. http://www.theses.fr/2017REIMS014/document.

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Le resvératrol, stilbène produit naturellement dans les grappes de raisin, possède un pouvoir anti-tumoral caractérisé par ses effets pléïotropiques. La molécule s’est ainsi montrée capable d’inhiber la cancérogenèse en agissant à de multiples niveaux. Son incidence sur la croissance tumorale in vitro est généralement associée à une perturbation dans le cycle cellulaire et à une induction d’apoptose. De nombreuses molécules dérivées du resvératrol se sont montrées également efficaces pour moduler la croissance tumorale. Nous avons entrepris au cours de ces travaux de thèse d’étudier les activi

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29

Bassani, Molinas Maria de los Milagros. "Transient transfection of HEK293 cells in suspension process characterization and optimization by applying invasive nucleotide and non-invasive electronic nose technology /." [S.l. : s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=976524295.

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30

Barbouche, Naziha. "Réponse biologique de cellules animales à des contraintes hydrodynamiques : simulation numérique, expérimentation et modélisation en bioréacteurs de laboratoire." Thesis, Vandoeuvre-les-Nancy, INPL, 2008. http://www.theses.fr/2008INPL075N/document.

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La réponse globale de cellules animales à des contraintes hydrodynamiques lors de leur culture en suspension dans des réacteurs agités a été étudiée grâce à une approche intégrative couplant les outils du génie biochimique à ceux de la mécanique des fluides numérique. En premier lieu, la description de l’hydrodynamique moyenne et locale de deux systèmes de culture agités de laboratoire, spinner et bioréacteur, a été réalisée. Puis, l'étude des cinétiques macroscopiques de cellules CHO cultivées en suspension, en milieu sans sérum et sans protéine, a été réalisée avec différentes vitesses d’agi

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31

Hall-Ponselè, Andrew M. "Genetic engineering of the primary/secondary metabolic interface in tobacco BY-2 cells." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:be5a3ee3-33c7-455c-b043-409987395f98.

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The supply of precursors from primary metabolism is often overlooked when engineering secondary metabolism for increased product yields. This is because precursor supply may be assumed to be non-limiting, and it is considered difficult to engineer primary metabolism, because control of carbon flow (flux) is generally distributed among most enzymes of the pathway. The aim of this thesis was to increase the production of sterols, part of the isoprenoid class of secondary metabolites, in tobacco (Nicotiana tabacum) Bright Yellow 2 (BY-2) cell cultures. This was achieved by genetically engineering

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32

Padre, Guilherme Augusto Del. "Estudo da cinética de crescimento de células de inseto Sf21 e infecção por baculovírus Anticarsia gemmatalis (AgMNPV) para a produção de bioinseticida." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/3/3137/tde-08072016-162745/.

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O interesse em estudar o cultivo das células de inseto está relacionado entre outros usos a sua utilização na produção de biopesticidas. Há muitos anos os pesticidas químicos vêm contribuindo no controle de pragas na agricultura. Entretanto, o uso desses compostos prolongadamente tem resultado na seleção de insetos resistentes e em poluição ambiental. Diante disso, torna-se necessário o desenvolvimento e aprimoramento dos bioinseticidas. No Brasil, o baculovírus Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) foi o principal agente de controle biológico da praga da soja Anticarsia

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33

Hill, Theresa Y. "Understanding Drop-on-Demand Inkjet Process Characteristics in the Application of Printing Micro Solid Oxide Fuel Cells." Wright State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=wright156167105938597.

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34

Menges, Margit. "Synchronisation of Arabidopsis cell suspension cultures." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620602.

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35

Leme, Jaci. "Cultivo de célula BHK-21 C13 em meio de cultura livre de soro fetal bovino adaptada para crescimento em suspensão." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/9/9134/tde-20022017-105416/.

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Células de mamíferos são os hospedeiros mais frequentemente utilizados para a fabricação de proteínas biofarmacêuticas e para a produção de vacinas virais, A qualidade é um elemento-chave para o estabelecimento de um processo de bioconversão eficiente. No presente trabalho utilizamos a linhagem de células BHK- 21C13(Baby Hamster Kidney) adaptadas para cultivo em suspensão. O uso de Soro Fetal Bovino (SFB) é tradicionalmente utilizado, sendo considerado um suplemento universal, pois permite o crescimento em várias linhagens de células de mamíferos; porém, uso de SFB apresenta risco de infecção

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36

Durand, Marjorie. "Effets de l’hypoxie respiratoire sur les progéniteurs médullaires dans un modèle murin d’hypodynamie : intérêt pour la réparation osseuse." Thesis, Paris 11, 2013. http://www.theses.fr/2013PA11T093/document.

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La réparation osseuse est assurée par le recrutement constant de cellules souches/progéniteurs ostéo-compétents de nature hématopoïétique (CSH/PH), et mésenchymateuse (CSM). Une approche prometteuse pour le traitement des défauts osseux graves consisterait à favoriser le recrutement et la mobilisation des CSH/PH et des CSM à partir de la moelle osseuse vers le site de lésion. Plusieurs facteurs environnementaux sont connus pour moduler la prolifération, la mobilisation et la différenciation des progéniteurs ostéocompétents, dont l’hypoxie et l’hypodynamie (absence de contraintes mécaniques). L

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37

Monteiro, Maria Beatriz de Oliveira. "Lignifica??o comparativa de Eucalyptus urophylla S. T. Blake por ferramentas biotecnol?gicas e polimeriza??o in vitro." Universidade Federal Rural do Rio de Janeiro, 2009. https://tede.ufrrj.br/jspui/handle/jspui/1211.

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Submitted by Leticia Schettini (leticia@ufrrj.br) on 2016-09-20T12:51:17Z No. of bitstreams: 1 2009 - Maria Beatriz de Oliveira Monteiro.pdf: 6872732 bytes, checksum: c5bedd487727835e97ae3e6b493349b3 (MD5)<br>Made available in DSpace on 2016-09-20T12:51:17Z (GMT). No. of bitstreams: 1 2009 - Maria Beatriz de Oliveira Monteiro.pdf: 6872732 bytes, checksum: c5bedd487727835e97ae3e6b493349b3 (MD5) Previous issue date: 2009-05-29<br>Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior-CAPES<br>In spite of the technological progresses, the understanding of the lignin structural formatio

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Ramm, Paul [Verfasser], and Hans Robert [Akademischer Betreuer] Kalbitzer. "High field 1H-NMR spectroscopy on cell suspensions of neural progenitor cells and brain tumor-initiating cells / Paul Ramm. Betreuer: Hans Robert Kalbitzer." Regensburg : Universitätsbibliothek Regensburg, 2011. http://d-nb.info/1023398796/34.

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MONTEIRO, Maria Beatriz de Oliveira. "Lignifica??o comparativa de Eucalyptus urophylla S. T. Blake por ferramentas biotecnol?gicas e polimeriza??o in vitro." Universidade Federal Rural do Rio de Janeiro, 2009. https://tede.ufrrj.br/jspui/handle/jspui/2025.

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Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-09-12T17:27:40Z No. of bitstreams: 1 2009 - Maria Beatriz de Oliveira Monteiro.pdf: 6859044 bytes, checksum: 68c2b98ad22b807fdd1e5779b401a4aa (MD5)<br>Made available in DSpace on 2017-09-12T17:27:40Z (GMT). No. of bitstreams: 1 2009 - Maria Beatriz de Oliveira Monteiro.pdf: 6859044 bytes, checksum: 68c2b98ad22b807fdd1e5779b401a4aa (MD5) Previous issue date: 2009-05-29<br>CAPES<br>In spite of the technological progresses, the understanding of the lignin structural formation is still matter of scientific investigations. This research

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Green, Martha Alexandra. "Apoplastic ascorbate metabolism in rose cell suspension cultures." Thesis, University of Edinburgh, 2003. http://hdl.handle.net/1842/14944.

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Endogenous intraprotoplasmic ascorbate in rose cell suspension cultures as a model system ranged from 0.05 mmol kg<sup>-1</sup> in 0-d-old cultures to 1.1 mmol kg<sup>-1</sup> in 5-d-old cultures. Apoplastic ascorbate was estimated as 0.5 and 8 μM in 0- and 5-d-old cultures respectively, indicating that ascorbate is endogenous to, and may be metabolised within, the apoplast. Exogenous (apoplastic) 1 mM L-[1-<sup>14</sup>C]ascorbate was almost completely consumed (metabolised and/or taken up) by rose cultures within 8 hours of administration. Total <sup>14</sup>C was removed from medium but slo

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髙橋, 宏二, and Koji Takahashi. "Calcium Signaling in Tobacco Suspension Culture Cells." Thesis, 1997. http://hdl.handle.net/2237/17057.

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42

Burton, James Dale. "Glyphosate uptake by suspension-cultured potato cells." 1986. http://catalog.hathitrust.org/api/volumes/oclc/15650674.html.

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Thesis (Ph. D.)--University of Wisconsin--Madison, 1986.<br>Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 160-171).

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43

Hou, Bi-Huei, and 侯碧惠. "Rapid Optimization of Electroporation Conditions for Soybean and Tomato Suspension Suspension Culture Cells." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/13517431022237216320.

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碩士<br>國立中興大學<br>植物學系<br>83<br>Two stains, Trypan blue and FDA were applied to obtain the optimum field strength in electroporation treatment on two plant cells, including protoplast, partially digested cells and suspension cells, all of them are dividedly acquired from soybean and tomato. The result shows the lowest field strength in electroporation happened to partially digested cells and the value is 0.5 KV/cm, followed by protoplast, 1KV/cm, and suspension cells, 1.5 KV/cm. Besides, the

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Fang, Jhen-Cheng, and 方振丞. "Functional Analysis of OsCAF1 Genes in Rice Suspension Cells." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/38084117073991286035.

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碩士<br>國立中央大學<br>生命科學研究所<br>99<br>One of central mechanisms to determine the expression level of genes for adjustment cellular status in response to various signals is messenger RNA (mRNA) degradation. Poly(A) tail deadenylation is a rate-limiting step of mRNA degradation. CCR4-associated factor 1s (CAF1s) is one of major enzymes to catalyze mRNA deadenylation in eukaryotes. Four members of CAF1 family, designated OsCAF1A, B, G and H, were analyzed and characterized in rice (Oryza sativa). Northern experiments and RT-PCR revealed diverse transcription patterns in response to sugar and various a

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LEE, CHIA-HUI, and 李佳蕙. "Salt-tolerant ability in rice (oryza sativa) L. suspension cells." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/50503290829715071565.

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Lin, Ming-Tsun, and 林明村. "Proteinase activity assay in rice suspension cells under sucrose starvation." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/58239085702618466056.

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碩士<br>國立嘉義大學<br>農學研究所<br>95<br>In order to verify the expression of the proteases activity in rice suspension cultured cells under sucrose-provided (+S) and -minus media (-S). After rice cells were cultured with or without sucrose for 1 to 5 days, the cells and media were collected, and total protein were isolated, respectively. The expression level of total protein in cells is higher when cells were cultured in +S than -S by SDS-PAGE analysis. About the total protein expression in medium, there were 2 groups of proteins, 30- and 37-kDa expressing in +S and -S media, but there were others prot

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Marpaung, David Septian Sumanto, and 馬大衛. "Expression of human Oct4 transcription factor in rice suspension cells." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/2g3yrt.

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碩士<br>元智大學<br>生物科技與工程研究所<br>106<br>Production of recombinant Octamer binding transcription factor (Oct4), a human embryonic transcription factor, gains interests due to high demands from generation of induced pluripotent stem cells in regenerative medical therapy recently. Up to date, no recombinant human transcription protein is produced in plant cells, which is known as safety hosts for recombinant protein production. In this study, an inducible expression of recombinant Oct4 by transformed rice suspension cell culture was established. The recombinant DNA insertion into rice chromosomes and

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Kuan-WeiChen and 陳冠瑋. "The role of thrombomodulin in regulating suspension growth of tumor cells." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/04508515677784275997.

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碩士<br>國立成功大學<br>生物化學暨分子生物學研究所<br>100<br>Thrombomodulin (TM), a cell surface glycoprotein, is well known as an anticoagulant which functions through binding with thrombin. Recent clinical studies indicate that the reduction of TM in cancer is associated with poor prognosis and tumor metastasis. However, the functional consequence of decreased TM expression in tumor metastasis remains unclear. To better understand the mechanism underlying the role of TM in tumor metastasis, HeLa cellswhich express heterogeneous TM were used. The sublines of HeLa cells with aggressive metastatic and anoikis-resis

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Chao, Chih-Hao, and 趙志豪. "Morphology Analysis of Suspension co-culture of Hs68 & HaCaT Cells." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/43473368796350471225.

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碩士<br>國立臺灣大學<br>醫學工程學研究所<br>101<br>We developed a new co-culture method mainly in co-culturing cells in suspension. Unlike the traditional method, cells cultured by this method will aggregate to a sphere and form a 3D structure, which compared to other systems like the hanging drop method and the hydrogel method, our system is simple, easy and good in long term culture. Our research used fibroblast and keratinocyte in co-culture, and we adopted Hs68 cell line from fibroblast and HaCaT cell line from keratinocyte. These cells are both from mature male foreskin, and the interaction existing bet

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Hsiao, Cheng Chung, and 蕭正忠. "Purification and Characterization of Insoluble Alkaline Invertase from Rice Suspension Cells." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/00865707926880470538.

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碩士<br>國立臺灣大學<br>農業化學研究所<br>87<br>A novel insoluble alkaline invertase was isolated from rice (Oryza sativa) suspension cells. The alkaline invertase (ITa) could be released by 1.0 M NaCl and purified further by ammonium sulfate fractionation, Con A-Sepharose affinity chromatography (non-retained), gel-filtration (Sepharose CL-6B) and DEAE-Sepharose chromatography. Another bound-form invertase (ITb) can be released by 5% EDTA from cell fragments after treated 1.0 M NaCl and purified further through the same steps. The molecular masses of ITa and ITb estimated by Superose 6 (FPLC) were 250 kD, a

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