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Journal articles on the topic 'Suspension cells'

Author: Grafiati

Published: 4 June 2021

Last updated: 3 February 2022

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1

Horie, Masanori, Haruhisa Kato, Shigehisa Endoh, et al. "Effects of Various Carbon Nanotube Suspensions on A549, THP-1, and Peritoneal Macrophage Cells." Journal of Biomimetics, Biomaterials and Biomedical Engineering 24 (July 2015): 1–13. http://dx.doi.org/10.4028/www.scientific.net/jbbbe.24.1.

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The effects of iron content, fiber length, and stability of carbon nanotube (CNT) suspension on cells were examined. Five kinds of single-wall carbon nanotube (SWCNT) suspensions were prepared: with catalytic iron, without iron, long SWCNTs (stable), short SWCNTs (stable), and short SWCNT (unstable). These suspensions were applied to A549, THP-1, and mouse peritoneal macrophage cells. After a 24-h exposure, the mitochondrial activity, cell membrane damage, intracellular oxidative stress, and expression of cytokine genes were determined. Among these properties of SWCNTs, stability of CNT suspen

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2

Oman, Srecko F., M. Filomena Camões, Kipton J. Powell, Raj Rajagopalan, and Petra Spitzer. "Guidelines for potentiometric measurements in suspensions Part B. Guidelines for practical pH measurements in soil suspensions (IUPAC Recommendations 2006)." Pure and Applied Chemistry 79, no. 1 (2007): 81–86. http://dx.doi.org/10.1351/pac200779010081.

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The measured cell potentials for suspension potentiometric cells have been interpreted and explained by a detailed analysis of the schemes for these cells ["Guidelines for potentiometric measurements in suspensions. Part A. The suspension effect (IUPAC Technical Report", Pure Appl. Chem.79, 67 (2007)]. Some former disagreements amongst investigations have been clarified. A new unambiguous operational definition of the suspension effect (SE) is presented. It is defined as the difference in cell potential for two suspension potentiometric cells, one with both electrodes in the separated equilibr

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3

Juckem,, Laura. "Transient Transfection of Suspension Cells." Genetic Engineering & Biotechnology News 31, no. 15 (2011): 26. http://dx.doi.org/10.1089/gen.31.15.11.

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4

Harlow, E. "Fixing Suspension Cells with Paraformaldehyde." Cold Spring Harbor Protocols 2006, no. 21 (2006): pdb.prot4295. http://dx.doi.org/10.1101/pdb.prot4295.

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5

Salazar, Alberto, Jane E. Nieto, Henry Velazquez-Soto, and Maria C. Jiménez-Martínez. "Activation of IL-10+ B cells: A novel immunomodulatory mechanism for therapeutic bacterial suspensions." SAGE Open Medicine 8 (January 2020): 205031212090154. http://dx.doi.org/10.1177/2050312120901547.

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Objectives: Bacterial components are used to improve immune responses in patients with respiratory infections. Pharmacological formulations of bacterial components include a mixture of bacterial antigens, some of which are complete inactivated bacteria, that is, named bacterial suspensions; while others are fragments of bacteria, which are presented as bacterial lysates. Although bacterial lysates have been broadly used as immune-stimulators, the biological support for the therapeutic effectiveness of bacterial suspension has not yet been studied. Thus, the aim of our study was to investigate

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6

Borodina, Irina, Boris Zaitsev, Andrey Teplykh, Gennady Burygin, and Olga Guliy. "Sensor Based on PZT Ceramic Resonator with Lateral Electric Field for Immunodetectionof Bacteria in the Conducting Aquatic Environment †." Sensors 20, no. 10 (2020): 3003. http://dx.doi.org/10.3390/s20103003.

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A biological sensor for detection and identification of bacterial cells, including a resonator with a lateral electric field based on PZT ceramics was experimentally investigated. For bacterial immunodetection the frequency dependencies of the electric impedance of the sensor with a suspension of microbial cells were measured before and after adding the specific antibodies. It was found that the addition of specific antibodies to a suspension of microbial cells led to a significant change in these frequency dependencies due to the increase in the conductivity of suspension. The analysis of mic

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7

LUPPENS, S. B. I., F. M. ROMBOUTS, and T. ABEE. "The Effect of the Growth Phase of Staphylococcus aureus on Resistance to Disinfectants in a Suspension Test." Journal of Food Protection 65, no. 1 (2002): 124–29. http://dx.doi.org/10.4315/0362-028x-65.1.124.

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The influence of growth phase on the resistance of Staphylococcus aureus to the surface-active agents benzalkonium chloride and dodecylbenzyl sulfonic acid and the oxidizing agents sodium hypochlorite and hydrogen peroxide was studied. The resistances of cells in different growth phases were compared to those of solid medium cells grown according to the European phase 1 suspension test. Using cells from different growth phases (±3 × 107 CFU ml−1), we found that decline-phase cells were the most resistant cells. However, the decline-phase cell suspension contained more than 90% dead cells. A 10

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8

Ruffoni, B., F. Massabo, and L. Volpi. "SUSPENSION CULTURES OF DIANTHUS CARYOPHYLLUS CELLS." Acta Horticulturae, no. 307 (August 1992): 251–56. http://dx.doi.org/10.17660/actahortic.1992.307.33.

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9

Tagesson, C., O. Stendahl, K. E. Magnusson, and L. Edebo. "DISINTEGRATION OF SINGLE CELLS IN SUSPENSION." Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology 81B, no. 4 (2009): 464–72. http://dx.doi.org/10.1111/j.1699-0463.1973.tb02231.x.

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10

Matsuura, Takanobu, Takami Kakuda, Tatsuyuki Kinoshita, Naokazu Takeuchi, and Kyosuke Sasaki. "Theanine Formation by Tea Suspension Cells." Bioscience, Biotechnology, and Biochemistry 56, no. 8 (1992): 1179–81. http://dx.doi.org/10.1271/bbb.56.1179.

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11

Galaction, Anca-Irina, Anca-Marcela Lupasteanu, and Dan Cascaval. "Bioreactors with stirred bed of immobilized cells, 2. Studies on distribution of mixing efficiency." Chemical Industry and Chemical Engineering Quarterly 13, no. 3 (2007): 135–50. http://dx.doi.org/10.2298/ciceq0703135g.

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The influences of the main factors on mixing efficiency and distribution for a bioreactor with stirred bed of S. cerevisiae immobilized cells in alginate have been analyzed. Indifferent of the region inside the suspension or of the biocatalyst volumetric fraction, the most efficient mixing has been obtained for biocatalyst particles with a 4.6 mm diameter. In function of the biocatalyst diameter and concentration, the uniform mixing can be reached in a whole bulk of suspension for certain rotation speed values. Therefore, for biocatalyst particles of 4 and 5.2 mm diameter, the suspensions have

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12

Gawrychowski, Krzysztof, Grzegorz Szewczyk, Ewa Skopińska-Różewska, et al. "The Angiogenic Activity of Ascites in the Course of Ovarian Cancer as a Marker of Disease Progression." Disease Markers 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/683757.

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Ovarian cancer cells are able to create invasive implants in the peritoneum and their growth is directly associated with the angiogenetic potential. This effect is probably stimulated by vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8), which are both found in ascites. The aim of this study was to assess the influence of ascites produced by ovarian cancer on the angiogenesis. Peritoneal fluid was collected from patients with advanced ovarian cancer; cancer cells were separated from CD45+ leukocytes. Angiogenesis was assessed in mice, after intradermal injection of full cellul

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13

Oman, Srecko F., M. Filomena Camões, Kipton J. Powell, Raj Rajagopalan, and Petra Spitzer. "Guidelines for potentiometric measurements in suspensions Part A. The suspension effect (IUPAC Technical Report)." Pure and Applied Chemistry 79, no. 1 (2007): 67–79. http://dx.doi.org/10.1351/pac200779010067.

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An explanation of the origin and interpretation of the suspension effect (SE) is presented in accordance with "pH Measurement: IUPAC Recommendations 2002" [Pure Appl. Chem.74, 2169 (2002)]. It is based on an analysis of detailed schemes of suspension potentiometric cells and confirmed with experimental results. Historically, the term "suspension effect" evolved during attempts to determine electrochemically the thermodynamically defined activity of H+ (aq) in suspensions. The experimental SE arises also in determining other pIon values, analogous to pH values.The SE relates to the observation

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14

Baldeck, Jeremiah D., and Robert E. Marquis. "Targets for hydrogen-peroxide-induced damage to suspension and biofilm cells of Streptococcus mutans." Canadian Journal of Microbiology 54, no. 10 (2008): 868–75. http://dx.doi.org/10.1139/w08-078.

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Hydrogen peroxide (H2O2) is considered a major endogenous source of oxidative stress to oral bacteria and also is widely used in oral care products. Our study objectives were to identify specific targets for H2O2-induced damage to cells of Streptococcus mutans in suspensions and monospecies biofilms and to differentiate bacteriostatic and bactericidal actions of the peroxide. Streptococcus mutans was grown in suspension cultures and fed-batch biofilms for assessing relative sensitivities of viability, glycolysis, and protein synthesis to H2O2 damage. Biofilm cells were found to have essentiall

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15

Castoldi, Lindsey, Lucinéia Reuse Albiero, Eduardo Figueredo Nery, Taiany Oliveira Kelly, Jeniffer Charlene Silva Dalazen, and Rosângela Guerino Masochini. "Evaluation of in vitro Cytotoxic Effects of Especifico Pessoa Phytotherapic Tincture on Ehrlich Tumor Cells and Mice Spleen Cells." Fronteiras: Journal of Social, Technological and Environmental Science 9, no. 1 (2020): 458–72. http://dx.doi.org/10.21664/2238-8869.2020v9i1.p458-472.

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Especifico Pessoa (EP) is traditionally used for the treatment of snakebite envenoming. The traditional use of EP and its properties have been reported. In this study, we evaluated the in vitro cytotoxic effects of EP on Ehrlich tumor and mice spleen cells. Cytotoxicity assay was carried out by using Trypan blue exclusion method. Spleen cell suspension was prepared (n=2) with RPMI medium and tumor cell suspension was prepared from ascitic fluid of Ehrlich tumor-bearing mice (n=1); both the suspensions contained 4 x 106 cells mL-1. Pure EP or EP diluted in RPMI (1:2; 1:4) was used. The results

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16

Pless-Petig, Gesine, Björn Walter, Anja Bienholz, and Ursula Rauen. "Mitochondrial Impairment as a Key Factor for the Lack of Attachment after Cold Storage of Hepatocyte Suspensions." Cell Transplantation 26, no. 12 (2017): 1855–67. http://dx.doi.org/10.1177/0963689717743254.

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Isolated primary hepatocytes, which are widely used for pharmacological and clinical purposes, usually undergo certain periods of cold storage in suspension during processing. While adherent hepatocytes were shown previously to suffer iron-dependent cell death during cold (4 °C) storage and early rewarming, we previously found little iron-dependent hepatocyte death in suspension but severely decreased attachment ability unless iron chelators were added. Here, we focus on the role of mitochondrial impairment in this nonattachment of hepatocyte suspensions. Rat hepatocyte suspensions were stored

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17

Janoschek, F., F. Mancini, J. Harting, and F. Toschi. "Rotational behaviour of red blood cells in suspension: a mesoscale simulation study." Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 369, no. 1944 (2011): 2337–44. http://dx.doi.org/10.1098/rsta.2011.0086.

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The nature of blood as a suspension of red blood cells makes computational haemodynamics a demanding task. Our coarse-grained blood model, which builds on a lattice Boltzmann method for soft particle suspensions, enables the study of the collective behaviour of the order of 10 6 cells in suspension. After demonstrating the viscosity measurement in Kolmogorov flow, we focus on the statistical analysis of the cell orientation and rotation in Couette flow. We quantify the average inclination with respect to the flow and the nematic order as a function of shear rate and haematocrit. We further rec

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18

Craig, B., L. Hawkey, and A. LeFurgey. "Techniques for cryoultramicrotomy of propane jet frozen biological samples." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 260–61. http://dx.doi.org/10.1017/s042482010014292x.

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Ultra-rapid freezing followed by cryoultramicrotomy is essential for the preservation of diffusible elements in situ within cells prior to scanning transmission electron microscopy and quantitative energy dispersive x-ray microanalysis. For cells or tissue fragments in suspension and for monolayer cell cultures, propane jet freezing provides cooling rates greater than 30,000°C/sec with regions up to 40μm in thickness free of significant ice crystal formation. While this method of freezing has frequently been applied prior to freeze fracture or freeze substitution, it has not been widely utiliz

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19

Takeishi, Naoki, Marco E. Rosti, Yohsuke Imai, Shigeo Wada, and Luca Brandt. "Haemorheology in dilute, semi-dilute and dense suspensions of red blood cells." Journal of Fluid Mechanics 872 (June 14, 2019): 818–48. http://dx.doi.org/10.1017/jfm.2019.393.

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We present a numerical analysis of the rheology of a suspension of red blood cells (RBCs) in a wall-bounded shear flow. The flow is assumed as almost inertialess. The suspension of RBCs, modelled as biconcave capsules whose membrane follows the Skalak constitutive law, is simulated for a wide range of viscosity ratios between the cytoplasm and plasma,$\unicode[STIX]{x1D706}=0.1$–10, for volume fractions up to$\unicode[STIX]{x1D719}=0.41$and for different capillary numbers ($Ca$). Our numerical results show that an RBC at low$Ca$tends to orient to the shear plane and exhibits so-called rolling

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20

Eilert, U., B. Wolters, and F. Constabel. "Ultrastructure of acridone alkaloid idioblasts in roots and cell cultures of Ruta graveolens." Canadian Journal of Botany 64, no. 6 (1986): 1089–96. http://dx.doi.org/10.1139/b86-149.

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Histological analysis of Ruta graveolens L. roots and in vitro grown cell suspensions revealed idioblasts with vacuoles containing clusters of droplets thought to be the storage compartment of acridone alkaloids. These idioblasts contained numerous vacuoles of varying sizes rather than the large, single, central vacuole characteristic of most adjacent parenchyma cells. The structure of idioblasts in roots and suspension cultures was identical. Treatment of suspension cultures with fungal elicitors known to increase alkaloid accumulation greatly did not affect the structure of idioblasts.

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21

Jones, K. H., and J. A. Senft. "An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide." Journal of Histochemistry & Cytochemistry 33, no. 1 (1985): 77–79. http://dx.doi.org/10.1177/33.1.2578146.

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A rapid, simultaneous double-staining procedure using fluorescein diacetate (FDA) and propidium iodide (PI) is described for use in the determination of cell viability in cell suspension. Air-dried slide preparations can be made from the cell suspensions so that an accurate estimate of the viability of the cells in the original suspension can be made up to 1 week later. Viable cells fluoresce bright green, while nonviable cells are bright red. Furthermore, when FDA-PI staining is compared to trypan blue dye exclusion as a method to determine cell viability, FDA-PI is found to be more consisten

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22

Stano, J., K. Mičieta, E. Tokhtaeva, M. Valšíková, M. Koreňová, and V. Blanáriková. "Demonstration of lactase activity in culture medium of melon cells." Horticultural Science 31, No. 4 (2011): 132–35. http://dx.doi.org/10.17221/3806-hortsci.

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Lactase activity was detected in a&nbsp;culture medium of the cell suspension culture of watermelon (Citrullus vulgaris L.). A&nbsp;simple, rapid and reproducible procedure for identification of extracellular lactase is described using callus cultures of seedlings from the tested plant, hairy roots of 2.5 days old seedlings of watermelon germinating on agar plates as well as cell suspension cultures derived from callus cultures. For the determination of intracellular activities of lactase, 6-bromo-2-naphthyl-&beta;-D-galactopyranoside and p-nitrophenyl-&beta;-D-galactopyranosid

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23

Randall, Stephen K., Mark S. Marshall, and Dring N. Crowell. "Protein Isoprenylation in Suspension-Cultured Tobacco Cells." Plant Cell 5, no. 4 (1993): 433. http://dx.doi.org/10.2307/3869723.

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24

Smith, Daniel, Chase Herman, Sidharth Razdan, Muhammad Raisul Abedin, William Van Stoecker, and Sutapa Barua. "Microparticles for Suspension Culture of Mammalian Cells." ACS Applied Bio Materials 2, no. 7 (2019): 2791–801. http://dx.doi.org/10.1021/acsabm.9b00215.

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25

Sakamoto, Shiho, Naoko Tsuchiya, Masanori Kuroyanagi, and Akira Ueno. "Biotransformation of germacrone by suspension cultured cells." Phytochemistry 35, no. 5 (1994): 1215–19. http://dx.doi.org/10.1016/s0031-9422(00)94823-4.

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26

Shang, Tanya Q., and Milton P. Gordon. "Transformation of [] trichloroethylene by poplar suspension cells." Chemosphere 47, no. 9 (2002): 957–62. http://dx.doi.org/10.1016/s0045-6535(02)00036-x.

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27

Krause, Maren, and Jörg Durner. "Harpin Inactivates Mitochondria in Arabidopsis Suspension Cells." Molecular Plant-Microbe Interactions® 17, no. 2 (2004): 131–39. http://dx.doi.org/10.1094/mpmi.2004.17.2.131.

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Harpin is a well-known proteinaceous bacterial elicitor that can induce an oxidative burst and programmed cell death in various host plants. Given the demonstrated roles of mitochondria in animal apoptosis, we investigated the effect of harpin from Pseudomonas syringae on mitochondrial functions in Arabidopsis suspension cells in detail. Fluorescence microscopy in conjunction with double-staining for reactive oxygen species (ROS) and mitochondria suggested co-localization of mitochondria and ROS generation. Plant defense responses or cell death after pathogen attack have been suggested to be r

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28

Graham, F. L. "Growth of 293 Cells in Suspension Culture." Journal of General Virology 68, no. 3 (1987): 937–40. http://dx.doi.org/10.1099/0022-1317-68-3-937.

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29

Perata, P., and A. Alpi. "Ethanol metabolism in suspension cultured carrot cells." Physiologia Plantarum 82, no. 1 (1991): 103–8. http://dx.doi.org/10.1034/j.1399-3054.1991.820115.x.

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30

Randall, S. K., M. S. Marshall, and D. N. Crowell. "Protein isoprenylation in suspension-cultured tobacco cells." Plant Cell 5, no. 4 (1993): 433–42. http://dx.doi.org/10.1105/tpc.5.4.433.

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31

Wissing, Josef, Sabina Heim, and Karl G. Wagner. "Diacylglycerol Kinase from Suspension Cultured Plant Cells." Plant Physiology 90, no. 4 (1989): 1546–51. http://dx.doi.org/10.1104/pp.90.4.1546.

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32

Wissing, Josef B., and Karl G. Wagner. "Diacylglycerol Kinase from Suspension Cultured Plant Cells." Plant Physiology 98, no. 3 (1992): 1148–53. http://dx.doi.org/10.1104/pp.98.3.1148.

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33

Pfeiffer, Wolfgang, and Margit Höftberger. "Oxidative burst in Chenopodium rubrum suspension cells:." Physiologia Plantarum 111, no. 2 (2001): 144–50. http://dx.doi.org/10.1034/j.1399-3054.2001.1110203.x.

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34

Takeda, Takumi, Yasushi Mitsuishi, Fukumi Sakai, and Takahisa Hayashi. "Xyloglucan Endotransglycosylation in Suspension-cultured Poplar Cells." Bioscience, Biotechnology, and Biochemistry 60, no. 12 (1996): 1950–55. http://dx.doi.org/10.1271/bbb.60.1950.

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35

Rodig, Scott J. "Attaching Suspension Cells to Slides for Staining." Cold Spring Harbor Protocols 2020, no. 12 (2020): pdb.prot099622. http://dx.doi.org/10.1101/pdb.prot099622.

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36

Perata, P., and A. Alpi. "Ethanol metabolism in suspension cultured carrot cells." Physiologia Plantarum 82, no. 1 (1991): 103–8. http://dx.doi.org/10.1111/j.1399-3054.1991.tb02909.x.

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37

Chan, Chii J., Andrew E. Ekpenyong, Stefan Golfier, et al. "Myosin II Activity Softens Cells in Suspension." Biophysical Journal 108, no. 8 (2015): 1856–69. http://dx.doi.org/10.1016/j.bpj.2015.03.009.

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38

Wu, Jianyong, Glenn King, Andreu J. Daugulis, Peter Faulkner, Derek H. Bone, and Mattheus F. A. Goosen. "Adaptation of insect cells to suspension culture." Journal of Fermentation and Bioengineering 70, no. 2 (1990): 90–93. http://dx.doi.org/10.1016/0922-338x(90)90277-4.

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39

Tramper, J., J. B. Williams, D. Joustra, and J. M. Vlak. "Shear sensitivity of insect cells in suspension." Enzyme and Microbial Technology 8, no. 1 (1986): 33–36. http://dx.doi.org/10.1016/0141-0229(86)90007-4.

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40

De Ryck, Leen M. H., J. B. Alexander Ross, Philip H. Petra, and Erlio Gurpide. "Estradiol entry into endometrial cells in suspension." Journal of Steroid Biochemistry 23, no. 2 (1985): 145–52. http://dx.doi.org/10.1016/0022-4731(85)90229-8.

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41

Emrich, Scott, and Han Chen. "Attachment of Suspension Cells for TEM Processing." Microscopy and Microanalysis 27, S1 (2021): 1396–97. http://dx.doi.org/10.1017/s1431927621005183.

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42

Ketchart, O., A. Treetong, P. Na-Ubon, and N. Supaka. "Determination the Effect of Silver Nanoparticles on Gram-Positive Bacterial Cells by Atomic Force Microscopy." Advanced Materials Research 506 (April 2012): 202–5. http://dx.doi.org/10.4028/www.scientific.net/amr.506.202.

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The atomic force microscope (AFM) was employed to study the significant effects of silver (Ag) nanoparticles-treated on the elastic cell wall of bacteria. In this study, the exposed Staphylococcus aureus was grown at 37 °C for 14 h. The cultures were centrifuged and cell pellets were resuspended in Milli-Q water to prepare final bacterial suspensions. A drop of bacterial suspension was deposited on polydimethylsiloxane (PDMS) sheet and allowed to air dry at room temperature before imaging. The cell suspension was collected at certain time intervals from the beginning of the test. The morpholog

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Nara, N., JE Curtis, JS Senn, DL Tritchler, and EA McCulloch. "The sensitivity to cytosine arabinoside of the blast progenitors of acute myeloblastic leukemia." Blood 67, no. 3 (1986): 762–69. http://dx.doi.org/10.1182/blood.v67.3.762.762.

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Abstract Two culture methods are available for the study of the blast cells of acute myeloblastic leukemia (AML). One is an assay for clonogenic precursors; it depends on their ability to form blast colonies in culture in the presence of methylcellulose and suitable growth factors. The other assesses the growth of blast cells in suspension culture, where growth is measured by increasing numbers of clonogenic cells. We have compared the two methods as assays for the cytotoxic effects of the chemotherapeutic drug cytosine arabinoside (Ara-C). Marked patient- to-patient variation was found using

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44

Nara, N., JE Curtis, JS Senn, DL Tritchler, and EA McCulloch. "The sensitivity to cytosine arabinoside of the blast progenitors of acute myeloblastic leukemia." Blood 67, no. 3 (1986): 762–69. http://dx.doi.org/10.1182/blood.v67.3.762.bloodjournal673762.

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Two culture methods are available for the study of the blast cells of acute myeloblastic leukemia (AML). One is an assay for clonogenic precursors; it depends on their ability to form blast colonies in culture in the presence of methylcellulose and suitable growth factors. The other assesses the growth of blast cells in suspension culture, where growth is measured by increasing numbers of clonogenic cells. We have compared the two methods as assays for the cytotoxic effects of the chemotherapeutic drug cytosine arabinoside (Ara-C). Marked patient- to-patient variation was found using either me

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45

Aronen, Tuija, Hely Häggman та Anja Hohtola. "Transient β-glucuronidase expression in Scots pine tissues derived from mature trees". Canadian Journal of Forest Research 24, № 10 (1994): 2006–11. http://dx.doi.org/10.1139/x94-257.

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Vegetative buds and bud-derived calli and suspension cells from 5- to 50-year-old Scots pines (Pinussylvestris L.) were used as targets for biolistic transformation. The gene construct used in the experiments was 35S CaMV–β-glucuronidase (GUS). The highest average level of transient GUS expression was found in suspension cells: 1229 ± 359 (mean ± SE) expressing cells per million. Transient expression was found in 35 of 44 (79%) tree genotypes studied. The expression level in buds and in calli was low: one or two spots per expressing bud. Growth-regulator pretreatment (BAP and 2,4-D) increased

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46

Negrete, Alejandro, Tau Chuan Ling, and Andrew Lyddiatt. "Production of Adenoviral Vectors in 293 Cells: A Case Study of the Adaptation of Attached Cells to Grow in Suspension." Open Biotechnology Journal 2, no. 1 (2008): 29–35. http://dx.doi.org/10.2174/1874070700802010029.

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A study of the production of adenoviral vectors in suspension 293 cells has been explored. A defined serumfree medium (293 SFM II) formulated without human or animal origin components from Invitrogen was used for the suspension adapted 293 cells. It was demonstrated that the 293 cells can be adapted to grow in suspension using serum free medium. The effect of different cell culture parameters was determined. The production technique demonstrated here is expected to simplify purification processes and circumvents the problems associated with serum containing medium.

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47

SI, Ignatova, Kušč LM, L. Bilisics, L. Trinh, Tiuliaeva NN, and Sedova MG. "Sucrase in immobilized cells of Cucumis sativus L." Horticultural Science 29, No. 1 (2012): 17–22. http://dx.doi.org/10.17221/4465-hortsci.

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Cell suspension cultures of Cucumis sativus L. &ndash; cucumber were permeabilized by Tween, hexadecyltri-methylammonium bromide, hexadecylpyridiniumbromide ethanol and/or immobilized by glutaraldehyde. The highest invertase activity was at pH 4.4 and temperature 53&deg;C. The hydrolysis of the substrate was linear for 5 h reaching 60% conversion. The cells displayed high sucrase activity and convenient physico-mechanical properties.

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Ab Kadir, Ruzanna, Shahrul Hisham Zainal Ariffin, Rohaya Megat Abdul Wahab, Shabnam Kermani, and Sahidan Senafi. "Characterization of Mononucleated Human Peripheral Blood Cells." Scientific World Journal 2012 (2012): 1–8. http://dx.doi.org/10.1100/2012/843843.

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Unspecialized cells that can renew themselves and give rise to multiple differentiated cell types are termed stem cells. The objective of this study was to characterize and investigate, through molecular and biochemical analyses, the stemness of cells derived from isolated mononucleated cells that originated from peripheral blood. The isolated mononucleated cells were separated according to their physical characteristics (adherent and suspension), after 4 to 7 days into a 14-day culturing period in complete medium. Our results revealed that adherent and suspension cells were positive for mesen

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Nara, N., and EA McCulloch. "The proliferation in suspension of the progenitors of the blast cells in acute myeloblastic leukemia." Blood 65, no. 6 (1985): 1484–93. http://dx.doi.org/10.1182/blood.v65.6.1484.bloodjournal6561484.

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Abstract A minority of blast cells in acute myeloblastic leukemia (AML) form colonies in culture in methylcellulose when stimulated by media conditioned by normal leukocytes in the presence of phytohemagglutinin (PHA-LCM). Blast colonies can be replated successfully, either as pooled cells or suspensions from single colonies. However, the plating efficiency declines with repeated passages, and more than four subcultures have not been achieved. In this study, blast populations were cultured in suspension, with fetal calf serum, alpha-minimal essential medium and PHA-LCM. In cells from 17 of 18

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Reiter, T., S. Penman, and D. G. Capco. "Shape-dependent regulation of cytoskeletal protein synthesis in anchorage-dependent and anchorage-independent cells." Journal of Cell Science 76, no. 1 (1985): 17–33. http://dx.doi.org/10.1242/jcs.76.1.17.

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Abstract:

We examine changes in protein synthesis that accompany suspension (i.e. shape alteration) of anchorage-dependent and anchorage-independent cells using a newly developed cell fractionation procedure based on detergent extraction. Using this procedure, a cell can be divided into four distinct and independent fractions: soluble, cytoskeleton, chromatin and nuclear matrix-intermediate filament. This fractionation procedure is used to investigate protein synthetic events associated with the release from anchorage-dependent growth, characteristic of transformed cells. Suspension results in several u

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