Relevant bibliographies by topics / SYBR Green RT-qPCR

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'SYBR Green RT-qPCR'

Author: Grafiati
Published: 2 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "SYBR Green RT-qPCR"

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Mahali, Helyatul Rasmah, and Nur Athirah Yusof. "Can Multiplex SYBR Green Real-Time PCR Assay Serve as a Detection and Quantification Method Comparable to the TaqMan Method for SARS-CoV-2 Diagnosis?" Borneo International Journal of Biotechnology (BIJB) 3 (December 22, 2023): 80–99. http://dx.doi.org/10.51200/bijb.v3i.4526.

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The reopening of schools, business, and social sectors during the COVID-19 pandemic has caused a current increase in the number of COVID-19 cases and clusters all over the globe. While the COVID-19 pandemic is far from over, the reopening and resumption of all economic sectors are essential to recovering the world economy. Health experts all over the world have determined that the real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) method is the gold standard for diagnosing COVID-19 infections due to the test’s high sensitivity and specificity. During the past 3 ye
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Tao, Yile, Yang Yue, Guangyu Qiu, et al. "Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR." Applied Microbiology and Biotechnology 106, no. 5-6 (2022): 2207–18. http://dx.doi.org/10.1007/s00253-022-11822-4.

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Abstract The pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For developing countries where vaccines are still in shortage, cheaper alternative molecular methods for SARS-CoV-2 identification can be crucial to prevent the next wave. Therefore, 14 primer sets recommended by the World Health Organization (WHO) was evaluated on testing both clinical patient and environmental samples with the gold standard diagnosis method, TaqMan-based RT-qPCR, and a cheaper alternative method, SYBR Green-based RT-qPCR. Using suitable primer sets, such as ORF1ab, 2019_nCoV_N1
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Dorlass, Erick Gustavo, Cairo Oliveira Monteiro, Amanda Oliveira Viana, et al. "Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR." Brazilian Journal of Microbiology 51, no. 3 (2020): 1117–23. http://dx.doi.org/10.1007/s42770-020-00347-5.

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Ståhlberg, Anders, Joakim Håkansson, Xiaojie Xian, Henrik Semb, and Mikael Kubista. "Properties of the Reverse Transcription Reaction in mRNA Quantification." Clinical Chemistry 50, no. 3 (2004): 509–15. http://dx.doi.org/10.1373/clinchem.2003.026161.

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Abstract Background: In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative measurements of gene expression. Methods: We used SYBR green I-based quantitative real-time PCR (QPCR) to measure the properties of reverse transcription reaction for the β-tubulin, glyceraldehyde-3-phosphate dehydrogenase, Glut2, CaV1D, and insulin II genes, using random hexamers, oligo(dT), and gene-specific reverse transcription primers. Results: Experimental variation in reverse transcription-QPCR (RT-
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Lau, Yee Ling, Ilyiana binti Ismail, Nur Izati binti Mustapa, et al. "Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)." PLOS ONE 16, no. 1 (2021): e0245164. http://dx.doi.org/10.1371/journal.pone.0245164.

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Rapid diagnosis is an important intervention in managing the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) outbreak. Real time reverse transcription polymerase chain reaction (RT-qPCR) remains the primary means for diagnosing the new virus strain but it is time consuming and costly. Recombinase polymerase amplification (RPA) is an isothermal amplification assay that does not require a PCR machine. It is an affordable, rapid, and simple assay. In this study, we developed and optimized a sensitive reverse transcription (RT)-RPA assay for the rapid detection of SARS-CoV-2 using SYB
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Picard-Meyer, Evelyne, Carine Peytavin de Garam, Jean Luc Schereffer, Clotilde Marchal, Emmanuelle Robardet, and Florence Cliquet. "Cross-Platform Evaluation of Commercial Real-Time SYBR Green RT-PCR Kits for Sensitive and Rapid Detection of European BatLyssavirusType 1." BioMed Research International 2015 (2015): 1–18. http://dx.doi.org/10.1155/2015/839518.

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This study evaluates the performance of five two-step SYBR Green RT-qPCR kits and five one-step SYBR Green qRT-PCR kits using real-time PCR assays. Two real-time thermocyclers showing different throughput capacities were used. The analysed performance evaluation criteria included the generation of standard curve, reaction efficiency, analytical sensitivity, intra- and interassay repeatability as well as the costs and the practicability of kits, and thermocycling times. We found that the optimised one-step PCR assays had a higher detection sensitivity than the optimised two-step assays regardle
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Gao, Shandian, Junzheng Du, Zhancheng Tian, et al. "A SYBR green I–based quantitative RT-PCR assay for bovine ephemeral fever virus and its utility for evaluating viral kinetics in cattle." Journal of Veterinary Diagnostic Investigation 32, no. 1 (2019): 44–50. http://dx.doi.org/10.1177/1040638719895460.

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We developed a SYBR green I–based reverse-transcription quantitative PCR (RT-qPCR) assay for bovine ephemeral fever virus (BEFV). Analytical sensitivity of the assay was ~ 100 times higher than conventional RT-PCR. The precision of the RT-qPCR established for RNA standards was high, with intra-assay and inter-assay coefficients of variation of 0.23–0.89% and 0.23–1.02%, respectively. The test was highly specific for BEFV strains, with no cross-reactivity with other viruses of veterinary significance. The assay detected BEFV RNA as early as 1 d post-infection (dpi) and up to 7–8 dpi in the bloo
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Koesharyani, Isti, Lila Gardenia, Tatik Mufidah, and Ayi Santka. "APLIKASI KUANTIFIKASI KOI HERPESVIRUS : REAL TIME – QUANTITATIVE POLYMERASE CHAIN REACTION (RT-Q PCR) MENGGUNAKAN SYBR GREEN PADA IKAN MAS (Cyprinus carpio)." Media Akuakultur 12, no. 1 (2017): 45. http://dx.doi.org/10.15578/ma.12.1.2017.45-53.

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Koi Herpes Virus (KHV) di Indonesia sejak tahun 2002 merupakan penyakit mematikan yang menyerang ikan koi Cyprinus carpio koi dan ikan mas Cyprinus carpio carpio, dan sampai saat ini, infeksi KHV dilaporkan sudah menyebar hampir di seluruh dunia. Untuk mengetahui adanya infeksi KHV perlu cara diagnosa yang sangat akurat/sensitif, sehingga keberadaan KHV dapat diketahui secara pasti dengan tingkat sensitivitas yang lebih baik pada ikan budidaya. Tujuan dari penelitian ini adalah untuk mengaplikasikan teknik deteksi dengan real time quantitative polymerase chain reaction (RT- qPCR/qPCR) guna men
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Vina-Rodriguez, Ariel, Konrad Sachse, Ute Ziegler, et al. "A Novel Pan-FlavivirusDetection and Identification Assay Based on RT-qPCR and Microarray." BioMed Research International 2017 (2017): 1–12. http://dx.doi.org/10.1155/2017/4248756.

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The genusFlavivirusincludes arthropod-borne viruses responsible for a large number of infections in humans and economically important animals. While RT-PCR protocols for specific detection of mostFlavivirusspecies are available, there has been also a demand for a broad-rangeFlavivirusassay covering all members of the genus. It is particularly challenging to balance specificity at genus level with equal sensitivity towards each target species. In the present study, a novel assay combining a SYBR Green-based RT-qPCR with a low-density DNA microarray has been developed. Validation experiments con
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Dráb, T., E. Svobodová, J. Ripl, et al. "SYBR Green I based RT-qPCR assays for the detection of RNA viruses of cereals and grasses." Crop and Pasture Science 65, no. 12 (2014): 1323. http://dx.doi.org/10.1071/cp14151.

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Less prevalent viruses of family Poaceae are usually excluded from the focus of interest, even though they represent a possible threat to agricultural production. We designed and validated a set of primer pairs suitable for detection and quantification of five RNA viruses, Lolium latent virus (LoLV), Oat necrosis mottle virus (ONMV), Ryegrass mosaic virus (RgMV), Soil-borne cereal mosaic virus (SBCMV), and Spartina mottle virus (SpMV), by means of one-step RT-qPCR based on SYBR Green I. These primers were used together with primers for Brome mosaic virus (BMV) and Wheat streak mosaic virus (WS
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Dissertations / Theses on the topic "SYBR Green RT-qPCR"

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Dahlin, Henrik. "Tidsserieanalys av aktiv norovirus-infektion med RT-qPCR." Thesis, Högskolan Kristianstad, Fakulteten för naturvetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-20077.

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Norovirus som orsakar vinterkräksjukan är en av de vanligaste vintersjukdomarna i Sverige. Sjukdomstiden varar generellt i en till tre dagar med symptomen kräkning och/eller diarré. Till den totala sjukdomsbilden världen över gällande akut gastroenterit, bidrar norovirus med 18 %. Trots att sjukdomen är mycket vanlig är kunskapen om norovirusets förfarande till stor del okänd.Syftet med studien var att göra en tidsserieanalys, även så kallad One-Step Growth analys, av koncentrationen minus-RNA i celler som infekterats med olika koncentrationer av murint norovirus (MNV). För att detektera minus
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Sharif, Sanaz. "Comparison of real-time PCR assays for screening of meticillin-resistant Staphylococcus aureus." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-154460.

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Staphylococcus aureus belongs to the normal flora. Many healthy people are colonized by the bacterium mainly in the nose but also on the skin and on other mucous membranes without showing symptoms. After damage to the skin, the bacterium can enter the wound and cause infections. Methicillin-resistant S. aureus (MRSA) is resistant to b-lactam antibiotics such as penicillin and methicillin. The gene that gives resistance characteristic of MRSA is the mecA-gene. MRSA strains are spread in both hospitals and in the community, and it is important to identify these bacteria with rapid and sensitive
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Book chapters on the topic "SYBR Green RT-qPCR"

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Vidalakis, Georgios, Jinbo Wang, Tyler Dang, et al. "SYBR® Green RT-qPCR for the Universal Detection of Citrus Viroids." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1464-8_18.

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Garcia, Beatriz Ferreira, Daniela Rodrigues Colpas, Andréia Moreira dos Santos Carmo, and Ivana Barros Campos. "USO DA METODOLOGIA RT-qPCR SYBR GREEN PARA DETECÇÃO DE VÍRUS RESPIRATÓRIOS." In Livro da V Mostra dos Trabalhos de Conclusão de Curso da Especialização em Vigilância Laboratorial em Saúde Pública. Agron Food Academy, 2024. http://dx.doi.org/10.53934/20242-18.

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Conference papers on the topic "SYBR Green RT-qPCR"

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Boldura, Oana Mari, Jelena Savici, Calin Mircu, Cornel Balta, and Simona Marc. "VALIDATION OF REFERENCE GENES FOR RT-QPCR IN PORCINE OOCYTES CULTURED IN ANTIOXIDANT-ENRICHED MEDIA." In 24th SGEM International Multidisciplinary Scientific GeoConference 2024. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024v/4.2/s19.52.

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Reference genes are essential for normalising data in RT-qPCR experiments and are critical in reducing technical variability. This study aimed to identify and validate stable reference genes for accurate gene expression analysis in porcine oocytes matured in culture media supplemented with antioxidants. Five commonly used reference genes were assessed: PPIA, RPL4, GAPDH, YWHAZ, and TBP, [2, 3] in oocytes cultured under oxidative stress conditions induced by Vitamin C. The experiment involved four experimental groups, analyzed in triplicate using SYBR Green RT-qPCR. PPIA exhibited the lowest SE
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YANG, Zexiao, Yadong LIU, Zhengqun MENG, et al. "Preliminary Study on The SYBR Green I RT- qPCR for Rabbit Hemorrhagic Disease Virus 2 (RHDV2) Detection." In International Conference on Biological Engineering and Pharmacy 2016 (BEP 2016). Atlantis Press, 2017. http://dx.doi.org/10.2991/bep-16.2017.9.

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Gu, Jiangping, Xiuwei Yue, Congli Yuan, et al. "Development and primary application of the SYBR Green I RT-qPCR assay for the detection of the transmissible gastroenteritis virus (TGEV) S gene." In 2011 4th International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2011. http://dx.doi.org/10.1109/bmei.2011.6098527.

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Remoto, Júlia Maranghetti, Maria Eduarda Ramos Cezine, Paulo Henrique Cavalcanti De Araújo, and Mariana Kiomy Osako. "EXPRESSÃO DE RANK-RANKL-OPG NA DIFERENCIAÇÃO DE CÉLULAS MUSCULARES ESQUELÉTICAS." In I Congresso Nacional On-line de Biologia Celular e Estrutural. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1947.

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Introdução: O sistema RANK-RANKL-OPG é constituído pelo receptor RANK, o ligante RANKL e o receptor solúvel Osteoprotegerina (OPG). Esse sistema tem importante papel na regulação de processos fundamentais ao tecido ósseo, como a remodelação óssea. A literatura descreve o papel da via de sinalização RANK-RANKL na regulação da atividade de retículos sarcoplasmáticos no tecido muscular, bem como a participação de OPG na redução de fraqueza muscular e restauração de fibras em casos de distrofia muscular em camundongos. Dessa forma, destaca-se a relevância de estudos sobre a unidade osso-músculo e
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