Log in

Relevant bibliographies by topics / SYBR Green RT-qPCR / Journal articles

To see the other types of publications on this topic, follow the link: SYBR Green RT-qPCR.

Journal articles on the topic 'SYBR Green RT-qPCR'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'SYBR Green RT-qPCR.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Mahali, Helyatul Rasmah, and Nur Athirah Yusof. "Can Multiplex SYBR Green Real-Time PCR Assay Serve as a Detection and Quantification Method Comparable to the TaqMan Method for SARS-CoV-2 Diagnosis?" Borneo International Journal of Biotechnology (BIJB) 3 (December 22, 2023): 80–99. http://dx.doi.org/10.51200/bijb.v3i.4526.

Full text

Abstract:

The reopening of schools, business, and social sectors during the COVID-19 pandemic has caused a current increase in the number of COVID-19 cases and clusters all over the globe. While the COVID-19 pandemic is far from over, the reopening and resumption of all economic sectors are essential to recovering the world economy. Health experts all over the world have determined that the real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) method is the gold standard for diagnosing COVID-19 infections due to the test’s high sensitivity and specificity. During the past 3 ye

APA, Harvard, Vancouver, ISO, and other styles

2

Tao, Yile, Yang Yue, Guangyu Qiu, et al. "Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR." Applied Microbiology and Biotechnology 106, no. 5-6 (2022): 2207–18. http://dx.doi.org/10.1007/s00253-022-11822-4.

Full text

Abstract:

Abstract The pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For developing countries where vaccines are still in shortage, cheaper alternative molecular methods for SARS-CoV-2 identification can be crucial to prevent the next wave. Therefore, 14 primer sets recommended by the World Health Organization (WHO) was evaluated on testing both clinical patient and environmental samples with the gold standard diagnosis method, TaqMan-based RT-qPCR, and a cheaper alternative method, SYBR Green-based RT-qPCR. Using suitable primer sets, such as ORF1ab, 2019_nCoV_N1

APA, Harvard, Vancouver, ISO, and other styles

3

Dorlass, Erick Gustavo, Cairo Oliveira Monteiro, Amanda Oliveira Viana, et al. "Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR." Brazilian Journal of Microbiology 51, no. 3 (2020): 1117–23. http://dx.doi.org/10.1007/s42770-020-00347-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Ståhlberg, Anders, Joakim Håkansson, Xiaojie Xian, Henrik Semb, and Mikael Kubista. "Properties of the Reverse Transcription Reaction in mRNA Quantification." Clinical Chemistry 50, no. 3 (2004): 509–15. http://dx.doi.org/10.1373/clinchem.2003.026161.

Full text

Abstract:

Abstract Background: In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative measurements of gene expression. Methods: We used SYBR green I-based quantitative real-time PCR (QPCR) to measure the properties of reverse transcription reaction for the β-tubulin, glyceraldehyde-3-phosphate dehydrogenase, Glut2, CaV1D, and insulin II genes, using random hexamers, oligo(dT), and gene-specific reverse transcription primers. Results: Experimental variation in reverse transcription-QPCR (RT-

APA, Harvard, Vancouver, ISO, and other styles

5

Lau, Yee Ling, Ilyiana binti Ismail, Nur Izati binti Mustapa, et al. "Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)." PLOS ONE 16, no. 1 (2021): e0245164. http://dx.doi.org/10.1371/journal.pone.0245164.

Full text

Abstract:

Rapid diagnosis is an important intervention in managing the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) outbreak. Real time reverse transcription polymerase chain reaction (RT-qPCR) remains the primary means for diagnosing the new virus strain but it is time consuming and costly. Recombinase polymerase amplification (RPA) is an isothermal amplification assay that does not require a PCR machine. It is an affordable, rapid, and simple assay. In this study, we developed and optimized a sensitive reverse transcription (RT)-RPA assay for the rapid detection of SARS-CoV-2 using SYB

APA, Harvard, Vancouver, ISO, and other styles

6

Picard-Meyer, Evelyne, Carine Peytavin de Garam, Jean Luc Schereffer, Clotilde Marchal, Emmanuelle Robardet, and Florence Cliquet. "Cross-Platform Evaluation of Commercial Real-Time SYBR Green RT-PCR Kits for Sensitive and Rapid Detection of European BatLyssavirusType 1." BioMed Research International 2015 (2015): 1–18. http://dx.doi.org/10.1155/2015/839518.

Full text

Abstract:

This study evaluates the performance of five two-step SYBR Green RT-qPCR kits and five one-step SYBR Green qRT-PCR kits using real-time PCR assays. Two real-time thermocyclers showing different throughput capacities were used. The analysed performance evaluation criteria included the generation of standard curve, reaction efficiency, analytical sensitivity, intra- and interassay repeatability as well as the costs and the practicability of kits, and thermocycling times. We found that the optimised one-step PCR assays had a higher detection sensitivity than the optimised two-step assays regardle

APA, Harvard, Vancouver, ISO, and other styles

7

Gao, Shandian, Junzheng Du, Zhancheng Tian, et al. "A SYBR green I–based quantitative RT-PCR assay for bovine ephemeral fever virus and its utility for evaluating viral kinetics in cattle." Journal of Veterinary Diagnostic Investigation 32, no. 1 (2019): 44–50. http://dx.doi.org/10.1177/1040638719895460.

Full text

Abstract:

We developed a SYBR green I–based reverse-transcription quantitative PCR (RT-qPCR) assay for bovine ephemeral fever virus (BEFV). Analytical sensitivity of the assay was ~ 100 times higher than conventional RT-PCR. The precision of the RT-qPCR established for RNA standards was high, with intra-assay and inter-assay coefficients of variation of 0.23–0.89% and 0.23–1.02%, respectively. The test was highly specific for BEFV strains, with no cross-reactivity with other viruses of veterinary significance. The assay detected BEFV RNA as early as 1 d post-infection (dpi) and up to 7–8 dpi in the bloo

APA, Harvard, Vancouver, ISO, and other styles

8

Koesharyani, Isti, Lila Gardenia, Tatik Mufidah, and Ayi Santka. "APLIKASI KUANTIFIKASI KOI HERPESVIRUS : REAL TIME – QUANTITATIVE POLYMERASE CHAIN REACTION (RT-Q PCR) MENGGUNAKAN SYBR GREEN PADA IKAN MAS (Cyprinus carpio)." Media Akuakultur 12, no. 1 (2017): 45. http://dx.doi.org/10.15578/ma.12.1.2017.45-53.

Full text

Abstract:

Koi Herpes Virus (KHV) di Indonesia sejak tahun 2002 merupakan penyakit mematikan yang menyerang ikan koi Cyprinus carpio koi dan ikan mas Cyprinus carpio carpio, dan sampai saat ini, infeksi KHV dilaporkan sudah menyebar hampir di seluruh dunia. Untuk mengetahui adanya infeksi KHV perlu cara diagnosa yang sangat akurat/sensitif, sehingga keberadaan KHV dapat diketahui secara pasti dengan tingkat sensitivitas yang lebih baik pada ikan budidaya. Tujuan dari penelitian ini adalah untuk mengaplikasikan teknik deteksi dengan real time quantitative polymerase chain reaction (RT- qPCR/qPCR) guna men

APA, Harvard, Vancouver, ISO, and other styles

9

Vina-Rodriguez, Ariel, Konrad Sachse, Ute Ziegler, et al. "A Novel Pan-FlavivirusDetection and Identification Assay Based on RT-qPCR and Microarray." BioMed Research International 2017 (2017): 1–12. http://dx.doi.org/10.1155/2017/4248756.

Full text

Abstract:

The genusFlavivirusincludes arthropod-borne viruses responsible for a large number of infections in humans and economically important animals. While RT-PCR protocols for specific detection of mostFlavivirusspecies are available, there has been also a demand for a broad-rangeFlavivirusassay covering all members of the genus. It is particularly challenging to balance specificity at genus level with equal sensitivity towards each target species. In the present study, a novel assay combining a SYBR Green-based RT-qPCR with a low-density DNA microarray has been developed. Validation experiments con

APA, Harvard, Vancouver, ISO, and other styles

10

Dráb, T., E. Svobodová, J. Ripl, et al. "SYBR Green I based RT-qPCR assays for the detection of RNA viruses of cereals and grasses." Crop and Pasture Science 65, no. 12 (2014): 1323. http://dx.doi.org/10.1071/cp14151.

Full text

Abstract:

Less prevalent viruses of family Poaceae are usually excluded from the focus of interest, even though they represent a possible threat to agricultural production. We designed and validated a set of primer pairs suitable for detection and quantification of five RNA viruses, Lolium latent virus (LoLV), Oat necrosis mottle virus (ONMV), Ryegrass mosaic virus (RgMV), Soil-borne cereal mosaic virus (SBCMV), and Spartina mottle virus (SpMV), by means of one-step RT-qPCR based on SYBR Green I. These primers were used together with primers for Brome mosaic virus (BMV) and Wheat streak mosaic virus (WS

APA, Harvard, Vancouver, ISO, and other styles

11

Bustin, Stephen, Harvinder S. Dhillon, Sara Kirvell, et al. "Variability of the Reverse Transcription Step: Practical Implications." Clinical Chemistry 61, no. 1 (2015): 202–12. http://dx.doi.org/10.1373/clinchem.2014.230615.

Full text

Abstract:

Abstract BACKGROUND The reverse transcription (RT) of RNA to cDNA is a necessary first step for numerous research and molecular diagnostic applications. Although RT efficiency is known to be variable, little attention has been paid to the practical implications of that variability. METHODS We investigated the reproducibility of the RT step with commercial reverse transcriptases and RNA samples of variable quality and concentration. We quantified several mRNA targets with either singleplex SYBR Green I or dualplex probe-based reverse transcription real-time quantitative PCR (RT-qPCR), with the

APA, Harvard, Vancouver, ISO, and other styles

12

Ren, Hengze, Yao Chen, Fumei Zhao, et al. "Quantitative Distribution and Transmission of Tea Plant Necrotic Ring Blotch Virus in Camellia sinensis." Forests 13, no. 8 (2022): 1306. http://dx.doi.org/10.3390/f13081306.

Full text

Abstract:

Tea plant necrotic ring blotch virus (TPNRBV), which carries four positive-sense single-stranded RNA segments, causes discoloration spots and multiple necrotic ring blotches in tea trees. To understand the distribution and transmission of TPNRBV in tea trees and prevent its spread, a SYBR Green real-time quantitative polymerase chain reaction (RT-qPCR) method for detecting the four virus segments was developed. The limit of detection of RT-qPCR was 3.81, 4.73, 3.58, and 4.64 copies/μL for the four strands of TPNRBV, which was 100-fold more sensitive than conventional PCR for RNA1 detection, 10

APA, Harvard, Vancouver, ISO, and other styles

13

De la Torre, David, Claudete Astolfi-Ferreira, Ruy Chacon, and Antonio Piantino Ferreira. "Sensitive SYBR Green—Real Time PCR for the Detection and Quantitation of Avian Rotavirus A." Veterinary Sciences 6, no. 1 (2018): 2. http://dx.doi.org/10.3390/vetsci6010002.

Full text

Abstract:

Avian rotavirus A (ARtV-A) is a virus that affects young birds, causing acute diarrhea and economic losses in the poultry industry worldwide. The techniques used for the diagnosis of ARtV-A include electron microscopy, isolation in cell culture, and serology, as well as molecular techniques, such as the reverse transcription-polymerase chain reaction (RT-PCR). The objective of this work was to standardize a real-time RT-polymerase chain reaction (RT-qPCR) using SYBR Green chemistry for the rapid detection and quantification of ARtV-A from bird tissues and materials fixed on FTA cards on the ba

APA, Harvard, Vancouver, ISO, and other styles

14

Olveira, José G., Sandra Souto, Isabel Bandín, and Carlos P. Dopazo. "Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards." Animals 11, no. 4 (2021): 1100. http://dx.doi.org/10.3390/ani11041100.

Full text

Abstract:

The nervous necrosis virus (NNV) is a threat to fish aquaculture worldwide, especially in Mediterranean countries. Fast and accurate diagnosis is essential to control it, and viral quantification is required to predict the level of risk of new viral detections in field samples. For both, reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) is used by diagnostic laboratories. In the present study, we developed an RT-qPCR procedure for the diagnosis and simultaneous quantification of NNV isolates from any of the four genotypes. The method proved to be highly sensitive

APA, Harvard, Vancouver, ISO, and other styles

15

Xiao, Jun, Xiaowei Li, Juan Liu, Xiu Fan, Huifen Lei, and Cuiying Li. "Identification of reference genes in blood before and after entering the plateau for SYBR green RT-qPCR studies." PeerJ 5 (September 27, 2017): e3726. http://dx.doi.org/10.7717/peerj.3726.

Full text

Abstract:

Background Tibetans have lived at high altitudes for thousands of years, and they have unique physiological traits that enable them to tolerate this hypoxic environment. However, the genetic basis of these traits is still unknown. As a sensitive and highly efficient technique, RT-qPCR is widely used in gene expression analyses to provide insight into the molecular mechanisms underlying environmental changes. However, the quantitative analysis of gene expression in blood is limited by a shortage of stable reference genes for the normalization of mRNA levels. Thus, systematic approaches were use

APA, Harvard, Vancouver, ISO, and other styles

16

Dacheux, L., F. Larrous, R. Lavenir, et al. "Dual Combined Real-Time Reverse Transcription Polymerase Chain Reaction Assay for the Diagnosis of Lyssavirus Infection." PLoS Negl Trop Dis 10, no. 7 (2016): e0004812. https://doi.org/10.5281/zenodo.13529791.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) The definitive diagnosis of lyssavirus infection (including rabies) in animals and humans is based on laboratory confirmation. The reference techniques for post-mortem rabies diagnosis are still based on direct immunofluorescence and virus isolation, but molecular techniques, such as polymerase chain reaction (PCR) based methods, are increasingly being used and now constitute the principal tools for diagnosing rabies in humans and for epidemiological analyses. However, it remains a key challenge to obtain relevant specificity and sensitivity w

APA, Harvard, Vancouver, ISO, and other styles

17

Dacheux, L., F. Larrous, R. Lavenir, et al. "Dual Combined Real-Time Reverse Transcription Polymerase Chain Reaction Assay for the Diagnosis of Lyssavirus Infection." PLoS Negl Trop Dis 10, no. 7 (2016): e0004812. https://doi.org/10.5281/zenodo.13529791.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) The definitive diagnosis of lyssavirus infection (including rabies) in animals and humans is based on laboratory confirmation. The reference techniques for post-mortem rabies diagnosis are still based on direct immunofluorescence and virus isolation, but molecular techniques, such as polymerase chain reaction (PCR) based methods, are increasingly being used and now constitute the principal tools for diagnosing rabies in humans and for epidemiological analyses. However, it remains a key challenge to obtain relevant specificity and sensitivity w

APA, Harvard, Vancouver, ISO, and other styles

18

Çelik, Ali, Deniz Çakar, Sibel Derviş, et al. "New Detection Methods for Cryphonectria Hypovirus 1 (CHV1) through SYBR Green-Based Real-Time PCR and Loop-Mediated Isothermal Amplification (LAMP)." Viruses 16, no. 8 (2024): 1203. http://dx.doi.org/10.3390/v16081203.

Full text

Abstract:

Some mycoviruses can be considered as effective biocontrol agents, mitigating the impact of phytopathogenic fungi and consequently reducing disease outbreaks while promoting plant health. Cryphonectria parasitica, the causal agent of chestnut blight and a highly destructive pathogen, experienced a notable decrease in its virulence with the identification of cryphonectria hypovirus 1 (CHV1), a naturally occurring biocontrol agent. In this study, two innovative diagnostic protocols designed for the accurate and efficient detection of CHV1 are introduced. The ORF A and ORF B regions of CHV1 are t

APA, Harvard, Vancouver, ISO, and other styles

19

Gao, Kai, Wasif Ullah Khan, Juan Li, et al. "Identification and Validation of Reliable Reference Genes for Gene Expression Studies in Koelreuteria paniculata." Genes 13, no. 5 (2022): 714. http://dx.doi.org/10.3390/genes13050714.

Full text

Abstract:

RT-qPCR is considered a rapid and reliable technique for analyzing gene expression. This technique is commonly used to analyze the expression of various genes at diverse transcriptional levels in different samples. However, few studies have characterized ornamental Koelreuteria species for reliable reference genes. In this study, eight reference genes were evaluated as controls in RT-qPCR with SYBR green to quantify gene expression in different Koelreuteria paniculata samples. All selected reference genes showed a broad range of Ct values in all samples, which was supportive of their variable

APA, Harvard, Vancouver, ISO, and other styles

20

Dong, Changying, Xingyu Xiao, Meiqi Wang, et al. "Development and Application of a TaqMan RT-qPCR for the Detection of Foot-and-Mouth Disease Virus in Pigs." Veterinary Sciences 11, no. 11 (2024): 541. http://dx.doi.org/10.3390/vetsci11110541.

Full text

Abstract:

The global livestock industry is facing a serious threat from a widespread foot-and-mouth disease virus (FMDV) epidemic. The timely detection of FMDV can significantly mitigate its harmful effects. This study aimed to establish and evaluate a TaqMan fluorescence quantitative PCR assay to assess its sensitivity, specificity, reproducibility, and stability. The standard curve equation range is 6.43 × 109–6.43 × 101 copies/µL, with an R2 value of 0.996 and a standard curve equation of y = −3.586x + 36.245. The method successfully detected 64.3 copies/µL of the target gene for FMDV and exhibited h

APA, Harvard, Vancouver, ISO, and other styles

21

Shah, Nilay, and Julia Selich-Anderson. "2139." Journal of Clinical and Translational Science 1, S1 (2017): 57. http://dx.doi.org/10.1017/cts.2017.205.

Full text

Abstract:

OBJECTIVES/SPECIFIC AIMS: (1) Correlate PBX1 mRNA expression as measured by RNAScope in situ hybridization, at an RNA number/cell measurement, Versus by RT-qPCR by the ddCt method. (2) Validate PBX1 mRNA expression in a second independent cohort of neuroblastoma tumor samples, and correlate with patient outcomes. We expect that PBX1 expression will correlate whether detected by RNAScope or by RT-qPCR. This work has the promise of validating a novel biomarker of disease severity, and for clinical translation as the RNAscope technology has been CLIA-certified for clinical use for other genes. ME

APA, Harvard, Vancouver, ISO, and other styles

22

Pozza, Lara, Sena Zumrutcu, Lizanne Bosman, et al. "Abstract 3780: From diagnostic labs to rapid near-patient testing: Bridging across RT-qPCR platforms of a clinicopathological and gene expression model for cutaneous melanoma patients." Cancer Research 84, no. 6_Supplement (2024): 3780. http://dx.doi.org/10.1158/1538-7445.am2024-3780.

Full text

Abstract:

Abstract Background: Gene expression signatures are becoming increasingly common in clinical settings, for diagnosis, risk stratification, and treatment response prediction. However, deployment in clinical practice often entails a molecular platform different from the one used for development. Transporting molecular signatures across platforms (“bridging”) cannot be achieved directly, due to differences in scales and distributions in gene expression and requires bespoke methods. Here, we focused on the bridging of a clinicopathological and gene expression model (CP-GEP) across two reverse tran

APA, Harvard, Vancouver, ISO, and other styles

23

Rahmasari, Ratika, Muhareva Raekiansyah, Syifa Naura Azallea, et al. "Low-cost SYBR Green-based RT-qPCR assay for detecting SARS-CoV-2 in an Indonesian setting using WHO-recommended primers." Heliyon 8, no. 11 (2022): e11130. http://dx.doi.org/10.1016/j.heliyon.2022.e11130.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Mayee, Khetam Qaid. "Some epidemiological features of BCoVs infection in Al-Qadisiyah Province by using real time-qPCR technique." Al-Qadisiyah Journal of Veterinary Medicine Sciences 13, no. 2 (2014): 14. http://dx.doi.org/10.29079/vol13iss2art296.

Full text

Abstract:

This study was carried out to evaluate some epidemiological features of Bovine Coronavirus infection by using one-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay based on SYBR Green I dye in detection. Coronaviruses detected by the same nucleocapsid (N) gene primers under 98% similarity with HECV-4408 (human enteric Coronavirus) in children according to NCBI with product size 124bp. 285 fecal samples have been examined by routine methods against pathogenic bacteria in the intestines (E.coli, Salmonella Spp.) and Cryptosporidium parvum, th

APA, Harvard, Vancouver, ISO, and other styles

25

Bogdanov, L. A., D. K. Shishkova, M. Yu Sinitsky, and A. G. Kutikhin. "Primer parameters defining efficiency and coefficient of determination in quantitative polymerase chain reaction." Complex Issues of Cardiovascular Diseases 9, no. 3 (2020): 13–20. http://dx.doi.org/10.17802/2306-1278-2020-9-3-13-20.

Full text

Abstract:

We performed a correlation analysis between primer parameters and qPCR efficiency/coefficient of determination in two independent samples from in vitro functional experiments.Primer parameters do not define qPCR efficiency and coefficient of determination significantly if primers are designed according to the optimised PRIMER-BLAST settings.Aim. To find the correlation between the primer parameters, efficiency, and coefficient of determination (R2 ) in quantitative polymerase chain reaction (qPCR) conditions.Methods. Upon RNA isolation from primary human coronary artery endothelial cells, we p

APA, Harvard, Vancouver, ISO, and other styles

26

REN, Fang, Zun-ping ZHANG, Xu-dong FAN, Guo-jun HU, Meng-yan ZHANG, and Ya-feng DONG. "A sensitive SYBR Green RT-qPCR method for grapevine virus E and its application for virus detection in different grapevine sample types." Journal of Integrative Agriculture 19, no. 7 (2020): 1834–41. http://dx.doi.org/10.1016/s2095-3119(19)62784-x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Tomkowiak, Agnieszka, Tomasz Jamruszka, Jan Bocianowski, et al. "Transcriptomic Characterization of Genes Harboring Markers Linked to Maize Yield." Genes 15, no. 12 (2024): 1558. https://doi.org/10.3390/genes15121558.

Full text

Abstract:

Background: It is currently believed that breeding priorities, including maize breeding, should focus on introducing varieties with greater utility value, specifically higher yields, into production. Global modern maize breeding relies on various molecular genetics techniques. Using the above mentioned technologies, we can identify regions of the genome that are associated with various phenotypic traits, including yield, which is of fundamental importance for understanding and manipulating these regions. Objectives: The aim of the study was to analyze the expression of candidate genes associat

APA, Harvard, Vancouver, ISO, and other styles

28

Zhao, Zhe, Yun Yu, Zhixiang Zhang, et al. "A duplex, SYBR Green I-based RT-qPCR assay for the simultaneous detection of Apple chlorotic leaf spot virus and Cherry green ring mottle virus in peach." Virology Journal 10, no. 1 (2013): 255. http://dx.doi.org/10.1186/1743-422x-10-255.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Dauch, A. L., B. Ahn, A. K. Watson, P. Seguin, and S. H. Jabaji-Hare. "Molecular Monitoring of Wild-Type and Genetically Engineered Colletotrichum coccodes Biocontrol Strains In Planta." Plant Disease 90, no. 12 (2006): 1504–10. http://dx.doi.org/10.1094/pd-90-1504.

Full text

Abstract:

Two strains of Colletotrichum coccodes, the wild type (DAOM 183088) and T-20a, engineered with the necrosis- and ethylene-inducing peptide (NEP1) gene for hypervirulence on velvetleaf (Abutilon theophrasti, Medik.), were monitored in planta for the first 2 weeks after infection. Real-time quantitative polymerase chain reaction (QPCR) was used to assess the extent of colonization of both strains on velvetleaf using SYBR Green chemistry. Quantification of both strains was successful as soon as the conidia were sprayed on the leaves and up to 14 days after infection. The increase in fungal DNA am

APA, Harvard, Vancouver, ISO, and other styles

30

Simões, C., H. Alakomi, J. Maukonen, and M. Saarela. "Expression of clpL1 and clpL2 genes in Lactobacillus rhamnosus VTT E-97800 after exposure to acid and heat stress treatments or to freeze-drying." Beneficial Microbes 1, no. 3 (2010): 253–57. http://dx.doi.org/10.3920/bm2010.0021.

Full text

Abstract:

The aim of the study was to evaluate the potential of utilising the information on expression levels of selected stress genes in assessing the quality of probiotic products. For this purpose RT-qPCR methods were developed to study the expression of clpL1 and clpL2 stress genes in Lactobacillus rhamnosus VTT E-97800 (E800) cells after exposure to processing-related stress conditions or to freeze-drying. Heat treatments in laboratory scale were performed with E800 cells incubated at 47 °C or 50 °C for 60 min. Acid treatments were performed both at laboratory and fermenter scale. At laboratory sc

APA, Harvard, Vancouver, ISO, and other styles

31

Arif, M., G. S. Aguilar-Moreno, A. Wayadande, J. Fletcher, and F. M. Ochoa-Corona. "Primer Modification Improves Rapid and SensitiveIn Vitroand Field-Deployable Assays for Detection of High Plains Virus Variants." Applied and Environmental Microbiology 80, no. 1 (2013): 320–27. http://dx.doi.org/10.1128/aem.02340-13.

Full text

Abstract:

ABSTRACTA high consequence pathogen,High plains virus(HPV) causes considerable damage to wheat if the crop is infected during early stages of development. Methods for the early, accurate, and sensitive detection of HPV in plant tissues are needed for the management of disease outbreaks and reservoir hosts. In this study, the effectiveness of five methods—real-time SYBR green and TaqMan reverse transcription-quantitative PCR (RT-qPCR), endpoint RT-PCR, RT-helicase dependent amplification (RT-HDA) and the Razor Ex BioDetection System (Razor Ex)—for the broad-range detection of HPV variants was e

APA, Harvard, Vancouver, ISO, and other styles

32

Abdel Sater, Fadi, Mahmoud Younes, Hassan Nassar, Paul Nguewa, and Kassem Hamze. "A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR." Molecular Biology Reports 48, no. 11 (2021): 7243–49. http://dx.doi.org/10.1007/s11033-021-06717-y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

OKINO, Cintia Hiromi, Maria de Fátima Silva MONTASSIER, Andressa Peres de OLIVEIRA, and Helio José MONTASSIER. "Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I." Journal of Veterinary Medical Science 80, no. 4 (2018): 725–30. http://dx.doi.org/10.1292/jvms.17-0566.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Marinho, A. N. R., D. C. C. Rocha, Y. K. Kanai, et al. "Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil." Journal of Water and Health 16, no. 6 (2018): 970–79. http://dx.doi.org/10.2166/wh.2018.130.

Full text

Abstract:

Abstract The contamination of mussels and oysters by viruses and bacteria is often associated with water contamination and gastroenteritis in humans. The present study evaluated viral and bacterial contamination in 380 samples, from nine mollusk-producing regions in coastal water north of the Brazilian Amazon. Rotavirus contamination was studied for groups A to H, using a two-step SYBR Green RT-qPCR (quantitative reverse transcription polymerase chain reaction), and bacterial families Enterobacteriaceae, Vibrionaceae, and Aeromonadaceae by classical and molecular methods. From the 19 pools ana

APA, Harvard, Vancouver, ISO, and other styles

35

Abdel Sater, Fadi, Mahmoud Younes, Hassan Nassar, Paul Nguewa, and Kassem Hamze. "Correction to: A rapid and low‑cost protocol for the detection of B.1.1.7 lineage of SARS‑CoV‑2 by using SYBR Green‑based RT‑qPCR." Molecular Biology Reports 49, no. 4 (2022): 3365. http://dx.doi.org/10.1007/s11033-021-07086-2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Laconi, Andrea, Alinda J. Berends, Esther C. H. de Laat, Tara A. P. M. P. Urselmann, and Hélène M. Verheije. "Infectious bronchitis virus Mass-type (GI-1) and QX-like (GI-19) genotyping and vaccine differentiation using SYBR green RT-qPCR paired with melting curve analysis." Journal of Virological Methods 275 (January 2020): 113771. http://dx.doi.org/10.1016/j.jviromet.2019.113771.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Medina Villaamil, Vanessa, Guadalupe Aparicio, Isabel Santamarina Cainzos, et al. "Peripheral blood microRNAs expression profile: A fingerprint for metastatic prostate cancer." Journal of Clinical Oncology 31, no. 15_suppl (2013): e22171-e22171. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.e22171.

Full text

Abstract:

e22171 Background: We previously have studied differentially microRNA expression levels related to hormone refractory and sensitive prostate cancer cell lines (VCap and LNCap respectively). Now, we investigated circulating miRNAs differentially expressed between metastatic prostate adenocarcinomas (mPA) and healthy controls (HC) that may serve as novel diagnostic and/or prognosis markers. Methods: Using SYBR-green-based custom microRNA RT-qPCR arrays technology (Exiqon), we compared the expression levels of miRNAs in blood samples from 18 HC and 48 mPA. Results: Among a panel of 92 candidates

APA, Harvard, Vancouver, ISO, and other styles

38

Arikawa, Emi, Min You, Jie Wang, Jing-yi Lo, Sean Yu, and Jingping Yang. "Real-time PCR array for simultaneous evaluation of multiple cytokine mRNA expression (B204)." Journal of Immunology 178, no. 1_Supplement (2007): LB42—LB43. http://dx.doi.org/10.4049/jimmunol.178.supp.b204.

Full text

Abstract:

Abstract Cytokine quantification is an important element in studies of inflammation and immune responses. Quantitative RT-PCR, a rapid and sensitive assay, is the preferred method to quantify cytokine mRNA levels because they are often expressed at low levels. The RT2Profiler™ PCR Array combines the reliable performance of SYBR® Green based RT-qPCR with multi-gene profiling capabilities to simultaneously analyze the expression of a panel of genes from the same pathway. Using PCR Arrays, we have monitored the mRNA levels of 84 different cytokines in human peripheral blood mononuclear cells (PBM

APA, Harvard, Vancouver, ISO, and other styles

39

Medina Villaamil, Vanessa, Guadalupe Aparicio Gallego, Francisco Gomez Veiga, et al. "Searching for circulating microRNAs in genitourinary tumors." Journal of Clinical Oncology 32, no. 4_suppl (2014): 468. http://dx.doi.org/10.1200/jco.2014.32.4_suppl.468.

Full text

Abstract:

468 Background: Detection of circulating tumor cells (CTC) may provide diagnostic and prognostic information in genitourinary tumors (GT). The aim of this work was identify aberrantly expressed miRNAs potentially useful for CTC detection in blood samples from patients with GT to assess their potential clinical significance, and to gain a greater understanding of the mechanisms driving tumor progression. Methods: We examined blood levels of 92 microRNAs in 113 metastatic patients: prostate (mP), renal cell carcinoma (mRCC), bladder tumors (mB) and healthy volunteers (HV) (N=18) using SYBR-green

APA, Harvard, Vancouver, ISO, and other styles

40

Hunt, Erin A., Sarah Schwartz, and Nicole Chinnici. "Passive Surveillance of SARS-CoV-2 in Adult Blacklegged Ticks (Ixodes scapularis) from Northeast Pennsylvania." Life 13, no. 9 (2023): 1857. http://dx.doi.org/10.3390/life13091857.

Full text

Abstract:

Monitoring the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wildlife is vital to public health. White-tailed deer (Odocoileus virginianus) in the United States have tested positive for SARS-CoV-2, and their interactions with blacklegged ticks (Ixodes scapularis) raise the question of whether or not these ticks also carry SARS-CoV-2. In this study, 449 blacklegged ticks from Northeast Pennsylvania were collected in the fall of 2022 and tested via RT-qPCR for the presence of SARS-CoV-2. Fourteen ticks were amplified with late quantification cycles (Cq) using primers

APA, Harvard, Vancouver, ISO, and other styles

41

Loor-Giler, Anthony, Sara Castillo-Reyes, Silvana Santander-Parra, et al. "First Report on the Molecular Detection of Canine Astrovirus (CaAstV) in Dogs with Gastrointestinal Disease in Ecuador Using a Fast and Sensitive RT-qPCR Assay Based on SYBR Green®." Veterinary Sciences 11, no. 7 (2024): 303. http://dx.doi.org/10.3390/vetsci11070303.

Full text

Abstract:

Enteric viruses are responsible for a significant number of gastrointestinal illnesses in dogs globally. One of the main enteric viruses is the canine astrovirus (CaAstV), which causes diarrhea in dogs of various ages. It is linked to symptoms such as diarrhea, vomiting, depression and a significant mortality rate due to gastrointestinal disorders. It is a single-stranded positive RNA virus, with three open reading frames, ORF1a, ORF1b and ORF2, where the last one codes for the virus capsid protein and is the most variable and antigenic region of the virus. The aim of this work is to develop a

APA, Harvard, Vancouver, ISO, and other styles

42

Pimenta, Yan Cardoso, Flávia Freitas de Oliveira Bonfim, Carlos Eduardo da Silva Figueiredo, et al. "Polymorphisms in the ACE I/D (rs4646994) and ACE2 G8790A (rs2285666) in Young Children Living in the Amazon Region and SARS-CoV-2 Infection." Tropical Medicine and Infectious Disease 9, no. 11 (2024): 270. http://dx.doi.org/10.3390/tropicalmed9110270.

Full text

Abstract:

COVID-19 infection caused by SARS-CoV-2 continues to cause significant mortality and morbidity. ACE2 is a key regulator of the renin–angiotensin–aldosterone system (RAAS). Differences in COVID-19 severity are thought to be due to the imbalance of RAAS/ACE mutations. This retrospective study evaluated the detection and genetic susceptibility to SARS-CoV-2 infection in 202 children ≤3 years of age living in the Amazon region in 2021. The angiotensin-converting enzyme ACE I/D (rs4646994) and ACE2 G8790A (rs2285666) polymorphisms were detected by SYBR GREEN real-time PCR and PCR-RFLP/Alul digestio

APA, Harvard, Vancouver, ISO, and other styles

43

Chrysanthopoulos, Ioannis, Despoina Mavrogianni, Eirini Drakaki, et al. "Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment." Journal of Clinical Medicine 12, no. 17 (2023): 5652. http://dx.doi.org/10.3390/jcm12175652.

Full text

Abstract:

Background: ZEB1 plays a role in epithelial-to-mesenchymal transition and acts as a repressor of E-cadherin, TGF-β, and Wnt/β-catenin. Since ZEB1 protein is expressed in estrogen-responsive tissues, and expression of the gene in the normal ovary and endometrium is positively correlated with high estrogen levels, we performed a direct analysis of granulosa cell samples to determine whether there are any significant changes in zeb1 expression during folliculogenesis. Methods: ZEB1 expression levels were measured in the granulosa cells of 56 infertile women undergoing IVF treatment. RNA extractio

APA, Harvard, Vancouver, ISO, and other styles

44

Valladares Ayerbes, Manuel, Vanessa Medina Villaamil, Sara Martinez Breijo, et al. "Circulating miR-337-3p as a novel biomarker for prostate cancer." Journal of Clinical Oncology 31, no. 15_suppl (2013): 5087. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.5087.

Full text

Abstract:

5087 Background: Recent studies have demonstrated that the aberrant expression of microRNAs (miRNAs)is related with the development of prostate cancer (PCa). Detection of circulating tumor cells (CTC) may provide diagnostic and prognostic information inPCa. The purpose is identifying circulating miRNAs potentially useful for CTC detection in patients with PCa. Methods: In the first study phase we examined blood levels of 92 miRNAs in 49 patients grouped in pools by risk classification: low-risk 42.8%, intermediate-risk 22.5% and high-risk 34.7% and healthy volunteers (N=10) using SYBR-green-ba

APA, Harvard, Vancouver, ISO, and other styles

45

Tsiakanikas, Panagiotis, Christos K. Kontos, Dimitrios Kerimis, Iordanis N. Papadopoulos, and Andreas Scorilas. "High microRNA-28-5p expression in colorectal adenocarcinoma predicts short-term relapse of node-negative patients and poor overall survival of patients with non-metastatic disease." Clinical Chemistry and Laboratory Medicine (CCLM) 56, no. 6 (2018): 990–1000. http://dx.doi.org/10.1515/cclm-2017-0430.

Full text

Abstract:

Abstract Background: MicroRNAs (miRNAs) may function either as oncogenes or tumor suppressors and are heavily involved in the initiation and progression of cancer, and in metastasis of tumor cells. MicroRNA-28-5p (miR-28-5p) targets several cancer-related genes and is hence involved in cell proliferation, migration, invasion and epithelial-mesenchymal transition. In this study, we investigated the potential diagnostic and prognostic significance of miR-28-5p expression in colorectal adenocarcinoma, the most frequent type of colorectal cancer (CRC). Methods: Therefore, we isolated total RNA fro

APA, Harvard, Vancouver, ISO, and other styles

46

Boldura, Oana-Maria, Simona Marc, and Călin Mircu. "Evaluation of Zona Pellucida Glycoproteins in Porcine Species: Gene Expression and Similarity Analysis." Romanian Journal of Veterinary Sciences 58, no. 1 (2024): 83–89. https://doi.org/10.59463/rjvs.2025.1.12.

Full text

Abstract:

This study examines the use of porcine zona pellucida (PZP) glycoproteins as targets for immunocontraceptive vaccines to con-trol wild boar populations. In this study, we assessed gene expression and sequence homology of five PZP glycoproteins (CD9, ITGa6, MFGE8, ZP2, and ZP3) in domestic pigs and wild boars to determine their suitability as immunocontraceptive targets. Oocytes were collected from both subspecies, and DNA and RNA were extracted for molecular analysis. Using end-point PCR for gene identification and RT-qPCR with SYBR Green for quantification, we measured expression levels acros

APA, Harvard, Vancouver, ISO, and other styles

47

Choi, Sujin, Minju Jung, Mingyoung Jeong, et al. "Postmortem Changes in mRNA Expression and Tissue Morphology in Brain and Femoral Muscle Tissues of Rat." International Journal of Molecular Sciences 26, no. 15 (2025): 7059. https://doi.org/10.3390/ijms26157059.

Full text

Abstract:

The postmortem interval (PMI), defined as the time elapsed between death and the discovery or examination of the body, is a crucial parameter in forensic science for estimating the time of death. There are many ways to measure the PMI, such as Henssge’s nomogram, which uses rectal temperature measurement; livor mortis; rigor mortis; and forensic entomology. However, these methods are usually affected by various conditions in the surrounding environment. The purpose of the present study was to compare molecular genetics and histological changes in the brain and skeletal muscle tissues of SD rat

APA, Harvard, Vancouver, ISO, and other styles

48

Diaz-Rubio, Gustavo Ignacio, Fernanda-Isadora Corona-Meraz, Perla-Monserrat Madrigal-Ruiz, et al. "CCR2/CCL2 and CMKLR1/RvE1 chemokines system levels are associated with insulin resistance in rheumatoid arthritis." PLOS ONE 16, no. 1 (2021): e0246054. http://dx.doi.org/10.1371/journal.pone.0246054.

Full text

Abstract:

Rheumatoid arthritis (RA) has been associated with insulin resistance (IR). Due to an excess in storage of white adipose tissue, IR has an inflammatory process that overlaps with RA. This is performed by the activation/migration of monocytes carried out by the CCR2/CCL2 and CMKLR1/RvE1 chemokines systems. Furthermore, these can potentiate chronic inflammation which is the central axis in the immunopathogenesis of RA. We evaluated the association between the relative expression of CCR2 and CMKLR1 and the serum levels of their ligands CCL2 and RvE1, in the context of adiposity status with IR as

APA, Harvard, Vancouver, ISO, and other styles

49

Andreone, Luz, Carolina Sétula, Juan Manuel Assad, and Marcelo Javier Perone. "O16 La activación de NRF2 por el compuesto A (CPDA) protege a las células-ß de la injuria inflamatoria mediada por citoquinas." Revista de la Sociedad Argentina de Diabetes 54, no. 3Sup (2020): 101. http://dx.doi.org/10.47196/diab.v54i3sup.463.

Full text

Abstract:

Introducción: el desarrollo del proceso autoinmune durante la diabetes tipo 1 (DM1) contribuye a la insulitis; en este contexto, el estrés de las células-ß y su posterior deficiencia en la secreción de insulina preceden a los signos clínicos de la enfermedad. La hiperglucemia desencadena la producción de especies reactivas de oxígeno (ROS) mitocondriales que superan la capacidad antioxidante de las células-ß conduciendo al estrés oxidativo. El microambiente inflamatorio del islote contribuye a la activación del estrés oxidativo y del retículo endoplásmico (RE) resultando en la disfunción y mue

APA, Harvard, Vancouver, ISO, and other styles

50

Andreone, Luz, Carolina Sétula, Juan Manuel Assad, and Marcelo Javier Perone. "O16 La activación de NRF2 por el compuesto A (CPDA) protege a las células-ß de la injuria inflamatoria medida por citoquinas." Revista de la Sociedad Argentina de Diabetes 54, no. 3Sup (2020): 101. http://dx.doi.org/10.47196/diab.v54i3sup.377.

Full text

Abstract:

Introducción: el desarrollo del proceso autoinmune durante la diabetes tipo 1 (DM1) contribuye a la insulitis; en este contexto, el estrés de las células-ß y su posterior deficiencia en la secreción de insulina preceden a los signos clínicos de la enfermedad. La hiperglucemia desencadena la producción de especies reactivas de oxígeno (ROS) mitocondriales que superan la capacidad antioxidante de las células-ß conduciendo al estrés oxidativo. El microambiente inflamatorio del islote contribuye a la activación del estrés oxidativo y del retículo endoplásmico (RE) resultando en la disfunción y mue

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!