Log in

Relevant bibliographies by topics / Synthetic promoter / Journal articles

To see the other types of publications on this topic, follow the link: Synthetic promoter.

Journal articles on the topic 'Synthetic promoter'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Synthetic promoter.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Tang, Hongting, Yanling Wu, Jiliang Deng, et al. "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae." Metabolites 10, no. 8 (2020): 320. http://dx.doi.org/10.3390/metabo10080320.

Full text

Abstract:

Promoters play an essential role in the regulation of gene expression for fine-tuning genetic circuits and metabolic pathways in Saccharomyces cerevisiae (S. cerevisiae). However, native promoters in S. cerevisiae have several limitations which hinder their applications in metabolic engineering. These limitations include an inadequate number of well-characterized promoters, poor dynamic range, and insufficient orthogonality to endogenous regulations. Therefore, it is necessary to perform promoter engineering to create synthetic promoters with better properties. Here, we review recent advances

APA, Harvard, Vancouver, ISO, and other styles

2

Gilman, James, and John Love. "Synthetic promoter design for new microbial chassis." Biochemical Society Transactions 44, no. 3 (2016): 731–37. http://dx.doi.org/10.1042/bst20160042.

Full text

Abstract:

The judicious choice of promoter to drive gene expression remains one of the most important considerations for synthetic biology applications. Constitutive promoter sequences isolated from nature are often used in laboratory settings or small-scale commercial production streams, but unconventional microbial chassis for new synthetic biology applications require well-characterized, robust and orthogonal promoters. This review provides an overview of the opportunities and challenges for synthetic promoter discovery and design, including molecular methodologies, such as saturation mutagenesis of

APA, Harvard, Vancouver, ISO, and other styles

3

Feng, Xiaofan, and Mario Andrea Marchisio. "Novel S. cerevisiae Hybrid Synthetic Promoters Based on Foreign Core Promoter Sequences." International Journal of Molecular Sciences 22, no. 11 (2021): 5704. http://dx.doi.org/10.3390/ijms22115704.

Full text

Abstract:

Promoters are fundamental components of synthetic gene circuits. They are DNA segments where transcription initiation takes place. New constitutive and regulated promoters are constantly engineered in order to meet the requirements for protein and RNA expression into different genetic networks. In this work, we constructed and optimized new synthetic constitutive promoters for the yeast Saccharomyces cerevisiae. We started from foreign (e.g., viral) core promoters as templates. They are, usually, unfunctional in yeast but can be activated by extending them with a short sequence, from the CYC1

APA, Harvard, Vancouver, ISO, and other styles

4

McWhinnie, Ralph L., and Francis E. Nano. "Synthetic Promoters Functional in Francisella novicida and Escherichia coli." Applied and Environmental Microbiology 80, no. 1 (2013): 226–34. http://dx.doi.org/10.1128/aem.02793-13.

Full text

Abstract:

ABSTRACTIn this work, we describe the identification of synthetic, controllable promoters that function in the bacterial pathogenFrancisella novicida, a model facultative intracellular pathogen. Synthetic DNA fragments consisting of the tetracycline operator (tetO) flanked by a random nucleotide sequence were inserted into aFrancisella novicidashuttle plasmid upstream of a promoterless artificial operon containing the reporter genescatandlacZ. Fragments able to promote transcription were selected for based on their ability to drive expression of thecatgene, conferring chloramphenicol resistanc

APA, Harvard, Vancouver, ISO, and other styles

5

Feng, Xiaofan, and Mario Andrea Marchisio. "Saccharomyces cerevisiae Promoter Engineering before and during the Synthetic Biology Era." Biology 10, no. 6 (2021): 504. http://dx.doi.org/10.3390/biology10060504.

Full text

Abstract:

Synthetic gene circuits are made of DNA sequences, referred to as transcription units, that communicate by exchanging proteins or RNA molecules. Proteins are, mostly, transcription factors that bind promoter sequences to modulate the expression of other molecules. Promoters are, therefore, key components in genetic circuits. In this review, we focus our attention on the construction of artificial promoters for the yeast S. cerevisiae, a popular chassis for gene circuits. We describe the initial techniques and achievements in promoter engineering that predated the start of the Synthetic Biology

APA, Harvard, Vancouver, ISO, and other styles

6

Efremova, Larisa N., Svetlana R. Strelnikova, Guzel R. Gazizova, Elena A. Minkina, and Roman A. Komakhin. "A Synthetic Strong and Constitutive Promoter Derived from the Stellaria media pro-SmAMP1 and pro-SmAMP2 Promoters for Effective Transgene Expression in Plants." Genes 11, no. 12 (2020): 1407. http://dx.doi.org/10.3390/genes11121407.

Full text

Abstract:

Synthetic promoters are vital for genetic engineering-based strategies for crop improvement, but effective methodologies for their creation and systematic testing are lacking. We report here on the comparative analysis of the promoters pro-SmAMP1 and pro-SmAMP2 from Stellaria media ANTIMICROBIAL PEPTIDE1 (AMP1) and ANTIMICROBIAL PEPTIDE2 (AMP2). These promoters are more effective than the well-known Cauliflower mosaic virus 35S promoter. Although these promoters share about 94% identity, the pro-SmAMP1 promoter demonstrated stronger transient expression of a reporter gene in Agrobacterium infi

APA, Harvard, Vancouver, ISO, and other styles

7

Wang, Ye, Haochen Wang, Lei Wei, Shuailin Li, Liyang Liu, and Xiaowo Wang. "Synthetic promoter design in Escherichia coli based on a deep generative network." Nucleic Acids Research 48, no. 12 (2020): 6403–12. http://dx.doi.org/10.1093/nar/gkaa325.

Full text

Abstract:

Abstract Promoter design remains one of the most important considerations in metabolic engineering and synthetic biology applications. Theoretically, there are 450 possible sequences for a 50-nt promoter, of which naturally occurring promoters make up only a small subset. To explore the vast number of potential sequences, we report a novel AI-based framework for de novo promoter design in Escherichia coli. The model, which was guided by sequence features learned from natural promoters, could capture interactions between nucleotides at different positions and design novel synthetic promoters in

APA, Harvard, Vancouver, ISO, and other styles

8

Blazeck, John, Rishi Garg, Ben Reed, and Hal S. Alper. "Controlling promoter strength and regulation inSaccharomyces cerevisiaeusing synthetic hybrid promoters." Biotechnology and Bioengineering 109, no. 11 (2012): 2884–95. http://dx.doi.org/10.1002/bit.24552.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Cazzonelli, Christopher Ian, and Jeff Velten. "In vivo characterization of plant promoter element interaction using synthetic promoters." Transgenic Research 17, no. 3 (2007): 437–57. http://dx.doi.org/10.1007/s11248-007-9117-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Matsumoto, Saki, Kei Iida, Asako Murata, Masatsugu Denawa, Masatoshi Hagiwara, and Kazuhiko Nakatani. "Synthetic ligand promotes gene expression by affecting GC sequence in promoter." Bioorganic & Medicinal Chemistry Letters 27, no. 15 (2017): 3391–94. http://dx.doi.org/10.1016/j.bmcl.2017.06.006.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Rud, Ida, Peter Ruhdal Jensen, Kristine Naterstad, and Lars Axelsson. "A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum." Microbiology 152, no. 4 (2006): 1011–19. http://dx.doi.org/10.1099/mic.0.28599-0.

Full text

Abstract:

A synthetic promoter library (SPL) for Lactobacillus plantarum has been developed, which generalizes the approach for obtaining synthetic promoters. The consensus sequence, derived from rRNA promoters extracted from the L. plantarum WCFS1 genome, was kept constant, and the non-consensus sequences were randomized. Construction of the SPL was performed in a vector (pSIP409) previously developed for high-level, inducible gene expression in L. plantarum and Lactobacillus sakei. A wide range of promoter strengths was obtained with the approach, covering 3–4 logs of expression levels in small increm

APA, Harvard, Vancouver, ISO, and other styles

12

Blazeck, John, Leqian Liu, Heidi Redden, and Hal Alper. "Tuning Gene Expression in Yarrowia lipolytica by a Hybrid Promoter Approach." Applied and Environmental Microbiology 77, no. 22 (2011): 7905–14. http://dx.doi.org/10.1128/aem.05763-11.

Full text

Abstract:

ABSTRACTThe development of strong and tunable promoter elements is necessary to enable metabolic and pathway engineering applications for any host organism. Here, we have expanded and generalized a hybrid promoter approach to produce libraries of high-expressing, tunable promoters in the nonconventional yeastYarrowia lipolytica. These synthetic promoters are comprised of two modular components: the enhancer element and the core promoter element. By exploiting this basic promoter architecture, we have overcome native expression limitations and provided a strategy for both increasing the native

APA, Harvard, Vancouver, ISO, and other styles

13

Rytter, Jakob Vang, Søren Helmark, Jun Chen, Mateusz Jakub Lezyk, Christian Solem, and Peter Ruhdal Jensen. "Synthetic promoter libraries for Corynebacterium glutamicum." Applied Microbiology and Biotechnology 98, no. 6 (2014): 2617–23. http://dx.doi.org/10.1007/s00253-013-5481-x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Campbell, A. Malcolm, Todd Eckdahl, Brian Cronk, et al. "pClone: Synthetic Biology Tool Makes Promoter Research Accessible to Beginning Biology Students." CBE—Life Sciences Education 13, no. 2 (2014): 285–96. http://dx.doi.org/10.1187/cbe.13-09-0189.

Full text

Abstract:

The Vision and Change report recommended genuine research experiences for undergraduate biology students. Authentic research improves science education, increases the number of scientifically literate citizens, and encourages students to pursue research. Synthetic biology is well suited for undergraduate research and is a growing area of science. We developed a laboratory module called pClone that empowers students to use advances in molecular cloning methods to discover new promoters for use by synthetic biologists. Our educational goals are consistent with Vision and Change and emphasize cor

APA, Harvard, Vancouver, ISO, and other styles

15

Buermeyer, A. B., N. E. Thompson, L. A. Strasheim, R. R. Burgess, and P. J. Farnham. "The HIP1 initiator element plays a role in determining the in vitro requirement of the dihydrofolate reductase gene promoter for the C-terminal domain of RNA polymerase II." Molecular and Cellular Biology 12, no. 5 (1992): 2250–59. http://dx.doi.org/10.1128/mcb.12.5.2250.

Full text

Abstract:

We examined the ability of purified RNA polymerase (RNAP) II lacking the carboxy-terminal heptapeptide repeat domain (CTD), called RNAP IIB, to transcribe a variety of promoters in HeLa extracts in which endogenous RNAP II activity was inhibited with anti-CTD monoclonal antibodies. Not all promoters were efficiently transcribed by RNAP IIB, and transcription did not correlate with the in vitro strength of the promoter or with the presence of a consensus TATA box. This was best illustrated by the GC-rich, non-TATA box promoters of the bidirectional dihydrofolate reductase (DHFR)-REP-encoding lo

APA, Harvard, Vancouver, ISO, and other styles

16

Buermeyer, A. B., N. E. Thompson, L. A. Strasheim, R. R. Burgess, and P. J. Farnham. "The HIP1 initiator element plays a role in determining the in vitro requirement of the dihydrofolate reductase gene promoter for the C-terminal domain of RNA polymerase II." Molecular and Cellular Biology 12, no. 5 (1992): 2250–59. http://dx.doi.org/10.1128/mcb.12.5.2250-2259.1992.

Full text

Abstract:

We examined the ability of purified RNA polymerase (RNAP) II lacking the carboxy-terminal heptapeptide repeat domain (CTD), called RNAP IIB, to transcribe a variety of promoters in HeLa extracts in which endogenous RNAP II activity was inhibited with anti-CTD monoclonal antibodies. Not all promoters were efficiently transcribed by RNAP IIB, and transcription did not correlate with the in vitro strength of the promoter or with the presence of a consensus TATA box. This was best illustrated by the GC-rich, non-TATA box promoters of the bidirectional dihydrofolate reductase (DHFR)-REP-encoding lo

APA, Harvard, Vancouver, ISO, and other styles

17

Martin, Craig T., and Joseph E. Coleman. "Kinetic analysis of T7 RNA polymerase-promoter interactions with small synthetic promoters." Biochemistry 26, no. 10 (1987): 2690–96. http://dx.doi.org/10.1021/bi00384a006.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Jores, Tobias, Jackson Tonnies, Travis Wrightsman, et al. "Synthetic promoter designs enabled by a comprehensive analysis of plant core promoters." Nature Plants 7, no. 6 (2021): 842–55. http://dx.doi.org/10.1038/s41477-021-00932-y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Righetti, Elena, Cansu Uluşeker, and Ozan Kahramanoğulları. "Stochastic Simulations as a Tool for Assessing Signal Fidelity in Gene Expression in Synthetic Promoter Design." Biology 10, no. 8 (2021): 724. http://dx.doi.org/10.3390/biology10080724.

Full text

Abstract:

The design and development of synthetic biology applications in a workflow often involve connecting modular components. Whereas computer-aided design tools are picking up in synthetic biology as in other areas of engineering, the methods for verifying the correct functioning of living technologies are still in their infancy. Especially, fine-tuning for the right promoter strength to match the design specifications is often a lengthy and expensive experimental process. In particular, the relationship between signal fidelity and noise in synthetic promoter design can be a key parameter that can

APA, Harvard, Vancouver, ISO, and other styles

20

Yang, Heping, Nathaniel Magilnick, Xiaopeng Ou та Shelly C. Lu. "Tumour necrosis factor α induces co-ordinated activation of rat GSH synthetic enzymes via nuclear factor κB and activator protein-1". Biochemical Journal 391, № 2 (2005): 399–408. http://dx.doi.org/10.1042/bj20050795.

Full text

Abstract:

GSH synthesis occurs via two enzymatic steps catalysed by GCL [glutamate–cysteine ligase, made up of GCLC (GCL catalytic subunit), and GCLM (GCL modifier subunit)] and GSS (GSH synthetase). Co-ordinated up-regulation of GCL and GSS further enhances GSH synthetic capacity. The present study examined whether TNFα (tumour necrosis factor α) influences the expression of rat GSH synthetic enzymes. To facilitate transcriptional studies of the rat GCLM, we cloned its 1.8 kb 5′-flanking region. TNFα induces the expression and recombinant promoter activities of GCLC, GCLM and GSS in H4IIE cells. TNFα i

APA, Harvard, Vancouver, ISO, and other styles

21

Guidry, C., and M. Hook. "Endothelins produced by endothelial cells promote collagen gel contraction by fibroblasts." Journal of Cell Biology 115, no. 3 (1991): 873–80. http://dx.doi.org/10.1083/jcb.115.3.873.

Full text

Abstract:

Endothelial 1 (E1) is identified as an endothelial cell secreted factor that stimulates collagen gel contraction by fibroblasts. This identification is based on (a) co-localization of stimulatory activity in endothelial cell conditioned media with synthetic E1 in reversed phase analysis; (b) removal of the activity from conditioned media with antiserum directed against E1; and (c) the activity of synthetic E1. Treatment of endothelial cell conditioned media with immobilized anti-E1 antibodies removed 59% of the activity from the pool suggesting that E1 is the major contraction promoter in endo

APA, Harvard, Vancouver, ISO, and other styles

22

Hammer, Karin, Ivan Mijakovic, and Peter Ruhdal Jensen. "Synthetic promoter libraries – tuning of gene expression." Trends in Biotechnology 24, no. 2 (2006): 53–55. http://dx.doi.org/10.1016/j.tibtech.2005.12.003.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Johari, Yusuf B., Adam J. Brown, Christina S. Alves, et al. "CHO genome mining for synthetic promoter design." Journal of Biotechnology 294 (March 2019): 1–13. http://dx.doi.org/10.1016/j.jbiotec.2019.01.015.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

He, Kevin, S. M. Ali Hosseini Rad, Aarati Poudel, and Alexander Donald McLellan. "Compact Bidirectional Promoters for Dual-Gene Expression in a Sleeping Beauty Transposon." International Journal of Molecular Sciences 21, no. 23 (2020): 9256. http://dx.doi.org/10.3390/ijms21239256.

Full text

Abstract:

Promoter choice is an essential consideration for transgene expression in gene therapy. The expression of multiple genes requires ribosomal entry or skip sites, or the use of multiple promoters. Promoter systems comprised of two separate, divergent promoters may significantly increase the size of genetic cassettes intended for use in gene therapy. However, an alternative approach is to use a single, compact, bidirectional promoter. We identified strong and stable bidirectional activity of the RPBSA synthetic promoter comprised of a fragment of the human Rpl13a promoter, together with additiona

APA, Harvard, Vancouver, ISO, and other styles

25

Sanches-Medeiros, Ananda, Lummy Maria Oliveira Monteiro, and Rafael Silva-Rocha. "Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators inEscherichia coli." International Journal of Genomics 2018 (2018): 1–10. http://dx.doi.org/10.1155/2018/9235605.

Full text

Abstract:

The engineering of synthetic circuits in cells relies on the use of well-characterized biological parts that would perform predicted functions under the situation considered, and many efforts have been taken to set biological standards that could define the basic features of these parts. However, since most synthetic biology projects usually require a particular cellular chassis and set of growth conditions, defining standards in the field is not a simple task as gene expression measurements could be affected severely by genetic background and culture conditions. In this study, we addressed pr

APA, Harvard, Vancouver, ISO, and other styles

26

McLean, Bradley W., Shari L. Wiseman, and Andrew M. Kropinski. "Functional analysis of sigma-70 consensus promoters in Pseudomonas aeruginosa and Escherichia coli." Canadian Journal of Microbiology 43, no. 10 (1997): 981–85. http://dx.doi.org/10.1139/m97-141.

Full text

Abstract:

A series of synthetic promoters, based upon the Escherichia coli σ70 consensus promoter sequence, was constructed upstream of the lacZ reporter gene in the modified broad-host-range vector pQF52. The role of the intervening spacer region in gene expression in Pseudomonas aeruginosa and E. coli was studied by insertions and deletions within this region. In P. aeruginosa and E. coli the patterns of gene expression were identical with maximum β-galactosidase activity being measured from promoters possessing 19 bp in their intervening regions, presumably as a result of impeded promoter clearance w

APA, Harvard, Vancouver, ISO, and other styles

27

Cai, Yao-Min, Kalyani Kallam, Henry Tidd, Giovanni Gendarini, Amanda Salzman, and Nicola J. Patron. "Rational design of minimal synthetic promoters for plants." Nucleic Acids Research 48, no. 21 (2020): 11845–56. http://dx.doi.org/10.1093/nar/gkaa682.

Full text

Abstract:

Abstract Promoters serve a critical role in establishing baseline transcriptional capacity through the recruitment of proteins, including transcription factors. Previously, a paucity of data for cis-regulatory elements in plants meant that it was challenging to determine which sequence elements in plant promoter sequences contributed to transcriptional function. In this study, we have identified functional elements in the promoters of plant genes and plant pathogens that utilize plant transcriptional machinery for gene expression. We have established a quantitative experimental system to inves

APA, Harvard, Vancouver, ISO, and other styles

28

Basak, Shashwati, and Valakunja Nagaraja. "DNA Unwinding Mechanism for the Transcriptional Activation ofmomP1Promoter by the Transactivator Protein C of Bacteriophage Mu." Journal of Biological Chemistry 276, no. 50 (2001): 46941–45. http://dx.doi.org/10.1074/jbc.m107476200.

Full text

Abstract:

Transcription factor-induced conformational changes in DNA are one of the mechanisms of transcription activation. C protein of bacteriophage Mu appears to transactivate themomgene of the phage by this mode. DNA binding by C to its site leads to torsional changes that seem to compensate for a weakmomP1 promoter having a suboptimal spacing of 19 bp between the poor −35 and −10 elements. The C-mediated unwinding could realign the promoter elements for RNA polymerase recruitment to the reoriented promoter. In this study, the model has been tested by mutational analysis of the spacer region ofmomP1

APA, Harvard, Vancouver, ISO, and other styles

29

Sinani, Devis, Ethan Cordes, Aspen Workman, Prasanth Thunuguntia, and Clinton Jones. "Stress-Induced Cellular Transcription Factors Expressed in Trigeminal Ganglionic Neurons Stimulate the Herpes Simplex Virus 1 ICP0 Promoter." Journal of Virology 87, no. 23 (2013): 13042–47. http://dx.doi.org/10.1128/jvi.02476-13.

Full text

Abstract:

Alphaherpesvirinaefamily members can reactivate from latency following stress. The synthetic corticosteroid dexamethasone induces certain cellular transcription factors in murine and bovine trigeminal ganglionic neurons. Three dexamethasone-induced transcription factors, Krüppel-like factor 15, Slug, and SPDEF, stimulated the herpes simplex virus type 1-infected cell protein 0 (ICP0) promoter more than 150-fold. Conversely, other viral promoters (VP16 and ICP4) were not strongly stimulated, suggesting that the ICP0 promoter is preferentially activated by dexamethasone-simulated stress.

APA, Harvard, Vancouver, ISO, and other styles

30

Xu, Jiangtao, Xiaoqing Liu, Xiaoxia Yu, Xiaoyu Chu, Jian Tian, and Ningfeng Wu. "Identification and characterization of sequence signatures in the Bacillus subtilis promoter Pylb for tuning promoter strength." Biotechnology Letters 42, no. 1 (2019): 115–24. http://dx.doi.org/10.1007/s10529-019-02749-4.

Full text

Abstract:

Abstract Objective To thoroughly characterize the Pylb promoter and identify the elements that affect the promoter activity. Result The sequences flanking the − 35 and − 10 box of the Pylb promoter were divided into six segments, and six random-scanning mutant promoter libraries fused to an enhanced green fluorescent protein EGFP were made and analyzed by flow cytometry. Our results showed that the four nucleotides flanking the − 35 box could mostly influence the promoter activity, and this influence was related to the GC content. The promoters mutated in these regions were successfully used f

APA, Harvard, Vancouver, ISO, and other styles

31

Kinkhabwala, Ali, and Călin C. Guet. "Uncovering cis Regulatory Codes Using Synthetic Promoter Shuffling." PLoS ONE 3, no. 4 (2008): e2030. http://dx.doi.org/10.1371/journal.pone.0002030.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Mordaka, Paweł M., and John T. Heap. "Stringency of Synthetic Promoter Sequences inClostridiumRevealed and Circumvented by Tuning Promoter Library Mutation Rates." ACS Synthetic Biology 7, no. 2 (2018): 672–81. http://dx.doi.org/10.1021/acssynbio.7b00398.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Chung, Y. T., and E. B. Keller. "Positive and negative regulatory elements mediating transcription from the Drosophila melanogaster actin 5C distal promoter." Molecular and Cellular Biology 10, no. 12 (1990): 6172–80. http://dx.doi.org/10.1128/mcb.10.12.6172.

Full text

Abstract:

The major cytoskeletal actin gene of Drosophila melanogaster, the actin 5C gene, has two promoters, the distal one of which controls synthesis of actin in a tissue- and developmental stage-specific manner. This very strong promoter has widely been used for expression of heterologous genes in cultured cells. To locate functional regulatory elements in this distal promoter, mutants of the promoter were fused to the bacterial chloramphenicol acetyltransferase gene and assayed for transient expression activity in cultured Drosophila embryonic Schneider line 2 cells. The results showed that the ups

APA, Harvard, Vancouver, ISO, and other styles

34

Chung, Y. T., and E. B. Keller. "Positive and negative regulatory elements mediating transcription from the Drosophila melanogaster actin 5C distal promoter." Molecular and Cellular Biology 10, no. 12 (1990): 6172–80. http://dx.doi.org/10.1128/mcb.10.12.6172-6180.1990.

Full text

Abstract:

The major cytoskeletal actin gene of Drosophila melanogaster, the actin 5C gene, has two promoters, the distal one of which controls synthesis of actin in a tissue- and developmental stage-specific manner. This very strong promoter has widely been used for expression of heterologous genes in cultured cells. To locate functional regulatory elements in this distal promoter, mutants of the promoter were fused to the bacterial chloramphenicol acetyltransferase gene and assayed for transient expression activity in cultured Drosophila embryonic Schneider line 2 cells. The results showed that the ups

APA, Harvard, Vancouver, ISO, and other styles

35

Gao, Yi-Zhou, Hong Liu, Hong-Jun Chao, and Ning-Yi Zhou. "Constitutive Expression of a Nag-Like Dioxygenase Gene through an Internal Promoter in the 2-Chloronitrobenzene Catabolism Gene Cluster of Pseudomonas stutzeri ZWLR2-1." Applied and Environmental Microbiology 82, no. 12 (2016): 3461–70. http://dx.doi.org/10.1128/aem.00197-16.

Full text

Abstract:

ABSTRACTThe gene cluster encoding the 2-chloronitrobenzene (2CNB) catabolism pathway inPseudomonas stutzeriZWLR2-1 is a patchwork assembly of a Nag-like dioxygenase (dioxygenase belonging to the naphthalene dioxygenase NagAaAbAcAd family fromRalstoniasp. strain U2) gene cluster and a chlorocatechol catabolism cluster. However, the transcriptional regulator gene usually present in the Nag-like dioxygenase gene cluster is missing, leaving it unclear how this cluster is expressed. The pattern of expression of the 2CNB catabolism cluster was investigated here. The results demonstrate that the expr

APA, Harvard, Vancouver, ISO, and other styles

36

Kim, Seong K., Randy A. Albrecht, and Dennis J. O'Callaghan. "A Negative Regulatory Element (Base Pairs −204 to −177) of the EICP0 Promoter of Equine Herpesvirus 1 Abolishes the EICP0 Protein's trans-Activation of Its Own Promoter." Journal of Virology 78, no. 21 (2004): 11696–706. http://dx.doi.org/10.1128/jvi.78.21.11696-11706.2004.

Full text

Abstract:

ABSTRACT The early EICP0 protein is a powerful trans-activator that activates all classes of equine herpesvirus 1 (EHV-1) promoters but, unexpectedly, trans-activates its own promoter very weakly. Transient transfection assays that employed constructs harboring deletions within the EICP0 promoter indicated that EICP0 cis-acting sequences within bp −224 to −158 relative to the first ATG abolished the EICP0 protein's trans-activation of its own promoter. When inserted into the promoters of other EHV-1 genes, this sequence also downregulated activation of the immediate-early IE(−169/+73), early t

APA, Harvard, Vancouver, ISO, and other styles

37

In, Solhee, Hyun-Ah Lee, Jongchan Woo, Eunsook Park, and Doil Choi. "Molecular Characterization of a Pathogen-Inducible Bidirectional Promoter from Hot Pepper (Capsicum annuum)." Molecular Plant-Microbe Interactions® 33, no. 11 (2020): 1330–39. http://dx.doi.org/10.1094/mpmi-07-20-0183-r.

Full text

Abstract:

In hot pepper, the sesquiterpene phytoalexin capsidiol is catalyzed by the two final-step enzymes, a sesquiterpene cyclase (EAS) and a hydroxylase (EAH), which are genetically linked and present as head-to-head orientation in the genome. Transcriptomic analysis revealed that a subset of EAS and EAH is highly induced following pathogen infection, suggesting the coregulation of EAS and EAH by a potential bidirectional activity of the promoter (pCaD). A series of the nested deletions of pCaD in both directions verified the bidirectional promoter activity of the pCaD. Promoter deletion analysis re

APA, Harvard, Vancouver, ISO, and other styles

38

Wang, W. D., and J. D. Gralla. "Differential ability of proximal and remote element pairs to cooperate in activating RNA polymerase II transcription." Molecular and Cellular Biology 11, no. 9 (1991): 4561–71. http://dx.doi.org/10.1128/mcb.11.9.4561.

Full text

Abstract:

To investigate the synergism or cooperative interaction between transcription elements, we have designed and constructed a series of synthetic polymerase II promoters with different combinations of elements. These include three different CCAAT boxes, which correspond to the binding sites for CP1, CP2, and NFI, a GC box, a CACCC box, and an ATF/CREB-binding site. The synthetic promoters containing these elements in proximal positions were linked to a test gene (CAT). Tandem repeats of AP1- and AP2-binding sites, the simian virus 40 enhancer, and DNA-binding sites for GAL-estrogen receptor were

APA, Harvard, Vancouver, ISO, and other styles

39

Wang, W. D., and J. D. Gralla. "Differential ability of proximal and remote element pairs to cooperate in activating RNA polymerase II transcription." Molecular and Cellular Biology 11, no. 9 (1991): 4561–71. http://dx.doi.org/10.1128/mcb.11.9.4561-4571.1991.

Full text

Abstract:

To investigate the synergism or cooperative interaction between transcription elements, we have designed and constructed a series of synthetic polymerase II promoters with different combinations of elements. These include three different CCAAT boxes, which correspond to the binding sites for CP1, CP2, and NFI, a GC box, a CACCC box, and an ATF/CREB-binding site. The synthetic promoters containing these elements in proximal positions were linked to a test gene (CAT). Tandem repeats of AP1- and AP2-binding sites, the simian virus 40 enhancer, and DNA-binding sites for GAL-estrogen receptor were

APA, Harvard, Vancouver, ISO, and other styles

40

Masson, Jean-Michel, and Jeffrey H. Miller. "Expression of synthetic suppressor tRNA genes under the control of a synthetic promoter." Gene 47, no. 2-3 (1986): 179–83. http://dx.doi.org/10.1016/0378-1119(86)90061-2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Searle, P. F., G. W. Stuart, and R. D. Palmiter. "Building a metal-responsive promoter with synthetic regulatory elements." Molecular and Cellular Biology 5, no. 6 (1985): 1480–89. http://dx.doi.org/10.1128/mcb.5.6.1480.

Full text

Abstract:

A fusion gene consisting of the promoter region from the mouse metallothionein-I gene joined to the coding region of the herpes simplex virus thymidine kinase gene is efficiently regulated by zinc in a transient assay when transfected into baby hamster kidney cells. Analysis of similar plasmids in which the metallothionein-I promoter region was mutated indicated the presence of multiple metal regulatory elements (MREs) between -176 and -44 base pairs from the cap site. To further investigate the function of MREs, we inserted a synthetic DNA fragment containing the sequence of MRE-a (the elemen

APA, Harvard, Vancouver, ISO, and other styles

42

Searle, P. F., G. W. Stuart, and R. D. Palmiter. "Building a metal-responsive promoter with synthetic regulatory elements." Molecular and Cellular Biology 5, no. 6 (1985): 1480–89. http://dx.doi.org/10.1128/mcb.5.6.1480-1489.1985.

Full text

Abstract:

A fusion gene consisting of the promoter region from the mouse metallothionein-I gene joined to the coding region of the herpes simplex virus thymidine kinase gene is efficiently regulated by zinc in a transient assay when transfected into baby hamster kidney cells. Analysis of similar plasmids in which the metallothionein-I promoter region was mutated indicated the presence of multiple metal regulatory elements (MREs) between -176 and -44 base pairs from the cap site. To further investigate the function of MREs, we inserted a synthetic DNA fragment containing the sequence of MRE-a (the elemen

APA, Harvard, Vancouver, ISO, and other styles

43

Sendy, Bandar, David J. Lee, Stephen J. W. Busby, and Jack A. Bryant. "RNA polymerase supply and flux through the lac operon in Escherichia coli." Philosophical Transactions of the Royal Society B: Biological Sciences 371, no. 1707 (2016): 20160080. http://dx.doi.org/10.1098/rstb.2016.0080.

Full text

Abstract:

Chromatin immunoprecipitation, followed by quantification of immunoprecipitated DNA, can be used to measure RNA polymerase binding to any DNA segment in Escherichia coli . By calibrating measurements against the signal from a single RNA polymerase bound at a single promoter, we can calculate both promoter occupancy levels and the flux of transcribing RNA polymerase through transcription units. Here, we have applied the methodology to the E. coli lactose operon promoter. We confirm that promoter occupancy is limited by recruitment and that the supply of RNA polymerase to the lactose operon prom

APA, Harvard, Vancouver, ISO, and other styles

44

Feng, Yingzhu, Zhangzhang Xie, Xuanlong Jiang, et al. "The Applications of Promoter-gene-Engineered Biosensors." Sensors 18, no. 9 (2018): 2823. http://dx.doi.org/10.3390/s18092823.

Full text

Abstract:

A promoter is a small region of a DNA sequence that responds to various transcription factors, which initiates a particular gene expression. The promoter-engineered biosensor can activate or repress gene expression through a transcription factor recognizing specific molecules, such as polyamine, sugars, lactams, amino acids, organic acids, or a redox molecule; however, there are few reported applications of promoter-enhanced biosensors. This review paper highlights the strategies of construction of promoter gene-engineered biosensors with human and bacteria genetic promoter arrays with regard

APA, Harvard, Vancouver, ISO, and other styles

45

Warren, Travis K., S. Amanda Lund, Kevin F. Jones, and Dennis E. Hruby. "Comparison of transformation protocols in Streptococcus gordonii and evaluation of native promoter strength using a multiple-copy plasmid." Canadian Journal of Microbiology 53, no. 3 (2007): 417–26. http://dx.doi.org/10.1139/w07-004.

Full text

Abstract:

An active area of research in the development of Streptococcus gordonii for use as a bacterial commensal vector involves the identification and utilization of strong promoters for high-level expression of heterologous products. Escherichia coli plasmid vectors containing different streptococcal promoters often fail to become established in E. coli for unknown reasons. Therefore, it is desirable at times to transform S. gordonii, which is naturally competent, with small quantities of nascently ligated DNA without using E. coli first to amplify or screen the product. By comparing the efficiency

APA, Harvard, Vancouver, ISO, and other styles

46

Guyer, Dave, Ann Tuttle, Sabrina Rouse, et al. "Activation of Latent Transgenes in Arabidopsis Using a Hybrid Transcription Factor." Genetics 149, no. 2 (1998): 633–39. http://dx.doi.org/10.1093/genetics/149.2.633.

Full text

Abstract:

Abstract A hybrid transcription factor comprising a fusion of the DNA-binding domain of Saccharomyces cerevisiae GAL4 and the transcription activation domain of maize C1 was expressed in stably transformed Arabidopsis. Additional transgenic lines were created containing test genes controlled by a synthetic promoter consisting of concatemeric copies of the cis-acting site recognized by GAL4 (UASG) fused to a minimal promoter. The GAL4/C1 effector line was crossed to two lines containing a synthetic promoter/GUS fusion. Both histochemical staining and GUS activity assays indicate strong activati

APA, Harvard, Vancouver, ISO, and other styles

47

FANIELLO, Maria C., Giuseppa CHIRICO, Barbara QUARESIMA, et al. "An alternative model of H ferritin promoter transactivation by c-Jun." Biochemical Journal 363, no. 1 (2002): 53–58. http://dx.doi.org/10.1042/bj3630053.

Full text

Abstract:

c-Jun is a member of the activator protein 1 family, and its interaction with different nuclear factors generates a wide spectrum of complexes that regulate transcription of different promoters. H ferritin promoter transcription is tightly dependent on nuclear factor Y (NFY). Ferritin transcription is activated by c-Jun, although the promoter does not contain a canonical binding site. NFY, on the other hand, does not bind c-Jun in vitro, whereas in vivo c-Jun is found in the complex containing NFY. Moreover, a c-Jun—GCN4 chimaeric construct containing only the transactivation domain of Jun and

APA, Harvard, Vancouver, ISO, and other styles

48

Bogenhagen, D. F., and M. F. Romanelli. "Template sequences required for transcription of Xenopus laevis mitochondrial DNA from two bidirectional promoters." Molecular and Cellular Biology 8, no. 7 (1988): 2917–24. http://dx.doi.org/10.1128/mcb.8.7.2917.

Full text

Abstract:

Previous work from our laboratory has shown that transcription of Xenopus laevis mitochondrial DNA initiates both in vivo and in vitro from bidirectional promoters located between the gene for tRNA(Phe) and the 5' termini of displacement loop DNA strands. A consensus sequence matching the octanucleotide ACGTTATA surrounds each transcription start site. In the present study, we used in vitro mutagenesis to define sequences required for specific transcription in vitro. First, cloned mitochondrial DNA templates generated by deletion mutagenesis were transcribed in vitro to define the limits of fu

APA, Harvard, Vancouver, ISO, and other styles

49

Bogenhagen, D. F., and M. F. Romanelli. "Template sequences required for transcription of Xenopus laevis mitochondrial DNA from two bidirectional promoters." Molecular and Cellular Biology 8, no. 7 (1988): 2917–24. http://dx.doi.org/10.1128/mcb.8.7.2917-2924.1988.

Full text

Abstract:

Previous work from our laboratory has shown that transcription of Xenopus laevis mitochondrial DNA initiates both in vivo and in vitro from bidirectional promoters located between the gene for tRNA(Phe) and the 5' termini of displacement loop DNA strands. A consensus sequence matching the octanucleotide ACGTTATA surrounds each transcription start site. In the present study, we used in vitro mutagenesis to define sequences required for specific transcription in vitro. First, cloned mitochondrial DNA templates generated by deletion mutagenesis were transcribed in vitro to define the limits of fu

APA, Harvard, Vancouver, ISO, and other styles

50

He, Weijing, Mei Qiang, Wuqiong Ma, et al. "Development of a Synthetic Promoter for Macrophage Gene Therapy." Human Gene Therapy 17, no. 9 (2006): 949–59. http://dx.doi.org/10.1089/hum.2006.17.949.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!