Academic literature on the topic 'Sysmex XN-550'

AbstractBackgroundBlood counters are primarily used to measure peripheral blood cells including platelets (PLTs). In routine quality control of platelet concentrates (PCs), counters are also used to analyze very high PLT counts. To meet the requirements of national and European guidelines for quality assurance, the accuracy of counting very high PLT counts has to be validated. The aim of the present study was to validate four blood counters (one of which has two detection methods) focusing on the PLT count.MethodsThe comparison was performed with PCs using the blood counter devices CELL-DYN Ruby (optical count) and CELL-DYN Emerald (impedance count), Sysmex K-4500 (impedance count), Sysmex XN-550 (impedance count) and Sysmex XN-550 (optical count). For precision performances, samples were measured serially 5 times and the coefficients of variation were calculated and compared with manufacturers’ requirements. Additionally, 50 peripheral blood samples were analyzed and standard hemogram parameters (red blood cells [RBC], white blood cells [WBC], hemoglobin [HGB], hematocrit [HCT], PLTs) were compared.ResultsThe comparison showed significant differences between the studied blood counter devices in measuring high PLT counts. The CELL-DYN-Emerald, the Sysmex K-4500 and Sysmex XN-500 with the optical counting method measured significantly higher PLT counts compared to the CELL-DYN-Ruby and the Sysmex XN-500 with the impedance counting technology (p<0.0001) independent of their principle of measurement. The manufacturers provide comparable coefficients of variation. We achieved similar results for all counters. All results of the peripheral blood count parameters were comparable.ConclusionsOur study showed the importance of blood counter validations focusing on PCs with high PLT counts before routine use. Not only the generally fundamental method, but also the manufacturers’ peculiarities seem to play an important role.

Background: In India, malaria has a major impact on health system. It is usually diagnosed based on symptomatology, parasite detection in the peripheral smear (PS) or rapid diagnostic tests (RDT) such as malaria antigen test (MAT). Detection of malaria by MAT is considered as the gold standard. A rapid, cost effective screening of malaria can be done with the automated analyzers. The present study was undertaken to assess the efficacy of WBC scattergram generated by Sysmex XN 550 hematology analyzer to diagnose malaria.
 Methods: A prospective study was conducted over a period of 4 months from August to November 2019, after obtaining institutional ethical clearance. All cases diagnosed as Plasmodium vivax / Plasmodium falciparum infections on malaria antigen test (MAT) were included. Their hemogram and WBC scattergrams obtained from Sysmex XN 550 were studied. Thick & thin Smears were made and stained with Leishman’s stain for microscopy.
 Results: A total of 101 cases were diagnosed as malaria positive by MAT and thick smear. Ninety-seven were positive by Leishman’s stain. Abnormal scattergrams were 81 out of 101 malaria positive cases. The commonest pattern was double neutrophil zone (n=22) followed by double neutrophil with less space between neutrophil and eosinophil (n=17). An abnormal event on X axis was observed in 16 patients. Gray zone and double eosinophil areas were observed in 11 and 4 cases respectively. The sensitivity of the analyzer was found to be 80.19%.
 Conclusion: Scattergram of automated haematology analyser (Sysmex XN 550) has good sensitivity, which can be increased to a better level if combined with thrombocytopenia and symptomatology of the patients.

Introduction:Clinical diagnostics in sudden-onset disasters (SOD) has historically been limited. With poor supply routes, lack of a cold chain, and challenging environmental conditions, many diagnostic platforms are unsuitable.Aim:We set out to design, implement, and evaluate a mobile diagnostic laboratory accompanying a type II emergency medical team (EMT) field hospital.Methods:Available diagnostic platforms were reviewed and selected against infield need. Platforms included HemoCue301/WBC DIFF, i-STAT, BioFire multiplex RT-PCR, Olympus BX53 microscopy, ABO/Rh Grouping, and specific rapid diagnostic tests (RDT). This equipment was trialed in Katherine, Australia and Dili, Timor-Leste.Results:During the initial deployment, validation of FilmArray rt-PCR multiplex tests was successful on blood culture, gastrointestinal, and respiratory panels. HemoCue301 (n = 20) haemoglobin values were compared on Sysmex XN 550 (r = 0.94). Analysis of HemoCue WBC DIFF samples had some variation when compared to Sysmex XN 550, (neutrophils r = 0.88, lymphocytes r = 0.49, monocytes r = 0.16, eosinophils r = 0.70, basophils r = 0.16). i-STAT showed non-significant differences for CHEM4 (n=10), CG8 (n = 10), and TnI (n = 5) against Vitros 250. A further trial of BioFire rt-PCR testing in Dili, Timor-Leste diagnosed 117 causative pathogens on 168 FilmArray test cartridges.Discussion:This mobile laboratory represents a major advance in SOD. Setup of the service was quick (<24hr) and transport to site rapidly. Training was simple and performance consistent. Future deployment in fragmented health systems after sudden onset disasters with EMT2 will now allow broader diagnostics.

Background When preparing dried blood spots (DBSs), haematocrit (Hct) can affect the ability of the blood to spread through the filter paper, thus resulting in varying quantities of sample being measured when fixed subpunches of the DBSs are taken. It may be important to predict the sample Hct to correct volume differences. Methods Blood (10 µL) was applied to Perkin Elmer 226® paper. The samples ( n = 165) were allowed to dry for 24 h, and the entire blood spots were cut out. Subpunch analysis was also performed on blood spots prepared from 75 µL EDTA blood, taking 6 mm subpunches centrally and peripherally from the spots ( n = 59). The spots were eluted with 100 µL water, and a 10 µL aliquot of lysate was added to sulfolyser reagent (80 µL) in a microtitre plate. Hb was measured at 550 nm using an ELISA plate reader. DBS samples were compared against blood samples measured on a routine Sysmex XN-9000 analyser. Results The Passing and Bablock regression showed Hct (DBS-predicted) = 0.99 Hct (Sysmex) −0.02, R2 = 0.87. Intra-assay imprecision measured at Hct values of 0.27, 0.40 and 0.52, gave CVs of 4.1%, 2.8% and 4.2%, respectively. Inter-assay imprecision showed CVs of 6.2%, 5.2% and 4.2%, respectively. DBS samples were stable for up to two days at 60℃, one month at room temperature and six months at 4℃. Conclusion This method provides a simple and fast estimation of predicted Hct in dried blood spots.

ABSTRACT Objectives: Clinical diagnostics in sudden onset disasters have historically been limited. We set out to design, implement, and evaluate a mobile diagnostic laboratory accompanying a type 2 emergency medical team (EMT) field hospital. Methods: Available diagnostic platforms were reviewed and selected against in field need. Platforms included HemoCue301/WBC DIFF, i-STAT, BIOFIRE FILMARRAY multiplex rt-PCR, Olympus BX53 microscopy, ABO/Rh grouping, and specific rapid diagnostic tests. This equipment was trialed in Katherine, Australia, and Dili, Timor-Leste. Results: During the initial deployment, an evaluation of FilmArray tests was successful using blood culture identification, gastrointestinal, and respiratory panels. HemoCue301 (n = 20) hemoglobin values were compared on Sysmex XN 550 (r = 0.94). HemoCue WBC DIFF had some variation, dependent on the cell, when compared with Sysmex XN 550 (r = 0.88-0.16). i-STAT showed nonsignificant differences against Vitros 250. Further evaluation of FilmArray in Dili, Timor-Leste, diagnosed 117 pathogens on 168 FilmArray pouches, including 25 separate organisms on blood culture and 4 separate cerebrospinal fluid pathogens. Conclusion: This mobile laboratory represents a major advance in sudden onset disaster. Setup of the service was quick (< 24 hr) and transport to site rapid. Future deployment in fragmented health systems after sudden onset disasters with EMT2 will now allow broader diagnostic capability.

Introduction and Objectives: In this study, we aimed to investigate whether platelet count (PLT) and platelet indices included mean platelet volume (MPV), platecrit (PCT), platelet distribution width (PDW) values can be used as diagnostic markers in cranial meningiomas.
 Materials and Methods: The study included results of 29 patient and 47 healthy contributors. Based on pathologies, the patients were divided into two groups. The first group included meningioma patients and the second one included healthy individuals. Healthy contributors named control group. Platelet count and platelet indices were determined using Sysmex XN 550 haematology analyzer. The preoperative platelet count (PLT) and platelet indices included mean platelet volume (MPV), platecrit (PCT), platelet distribution width (PDW) values were recorded from the routine laboratory tests.
 Results: There was no statistically significant difference in PLT between the meningioma and healthy groups (p = 0.217). There was a statistically significant difference in PCT between the meningioma group and the healthy group (p = 0.002). There was a statistically significant difference in PDW between meningioma group and healthy group (p = 0.001). In terms of MPV, there was a statistically significant difference between meningioma group and the healthy group (p = 0.001)
 Conclusion: Platelet count and indices are easily available in the routine blood tests. Despite the retrospective design and small sample size, our findings suggest that altered MPV, PDW and PCT levels might serve as potential biomarkers for the diagnosis of meningiomas.

Färsk fryst plasma (FFP) är en blodkomponent som utvinns genom separation från helblod. FFP innehåller livsviktiga plasmaproteiner, immunoglobuliner och koagulationsfaktorer. Produkten används främst till patienter med omfattande skador som lett till större blodförlust samt vid större kirurgiska blödningar vid operationer. Enligt riktlinjer stiftade av European Directorate for the quality of Medicines and healtcare (EDQM) bör samtliga plasmaenheter understiga 6,0 × 109/L erytrocyter för att produkten klassificeras som godtagbar för transfusion. Syftet med denna studie var att undersöka om Sysmex XN-550 med applikation ”Body Fluid” (BF) kan tillförlitligt koncentrationsbestämma erytrocyter i FFP och därmed ersätta Bürkerkammare som är den nuvarande metoden för dessa mätningar. För detta ändamål utfördes en undersökning av linjäritet hos instrumentet, en statistisk jämförelse med mätning av 30 prover samt fem upprepade mätningar av en känd koncentration för undersökning av reproducerbarheten. Resultaten visade en tydlig korrelation mellan analyserna med en jämn stegring av värden. Korrelationskoefficienten erhöll ett värde på 0,99. Bland Altmananalys visade att 95% av uppmätta värden förelåg inom konfidensintervallet vilket påvisar en överensstämmelse mellan metoderna med p-värde på 0,98. Mätningarna påvisade p-värden på 0,0005 vid analys av den lägsta koncentrationen (1 × 109/L), 0,5 vid anays av näst högsta koncentrationen (3 × 109/L) samt 0,9 vid analys av den högsta koncentrationen (6 × 109/L).<br>Fresh frozen plasma (FFP) is a blood component derived from separation of whole blood. FFP contains essential plasma proteins, immunoglobulins and coagulation factors. The product is mainly used for patients with extensive injuries which have led to major blood loss or for major bleeding due to surgical procedures. According to guidelines established by the European Directorate for the quality of Medicines and healtcare (EDQM), all plasma components should have an erythrocyte concentration of less than 6 × 109/L for the product to be classified as acceptable for transfusion. The purpose of this study was to investigate whether Sysmex XN-550 with the application “Body Fluid” (BF) could reliably determine the concentration of erythrocytes in FFP and thus replace the current method of Bürker chamber for these measurements. For this purpose, the linearity of the instruments was studied and a statistical comparison including measurements of 30 samples and five repeated measurements of a known concentration for examination of the reproducibility. The results showed a clear correlation between the analyses with an even increase in value. The correlation coefficient provided a value of 0,99. Bland Altman analysis showed that 95% of the measured values were within the confidence interval, which shows a correlation between the used methods and the calculated p-value was 0.98. The reproducibility measurements revealed a p-value of 0,0005 when measuring the lowest concentration (1 × 109/L), 0,5 when measuring the second to highest concentration (3 × 109/L) and 0,9 when measuring the highest concentration (6 × 109/L).