Academic literature on the topic 'System-active approach'
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Journal articles on the topic "System-active approach"
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Avramenko, Victor Vasilevich. "System-active approach in learning German grammar." Вестник Шадринского государственного педагогического университета, no. 2 (2021): 10–13. http://dx.doi.org/10.52772/25420291_2021_2_10.
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Nimdia, Kirit, and Balasubrahmanya Balakrishna. "Batch Job Active-Active Resiliency System and Method." International Journal of Computer Science and Mobile Computing 13, no. 5 (2024): 89–99. http://dx.doi.org/10.47760/ijcsmc.2024.v13i05.008.
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This manuscript presents a robust solution for orchestrating batch job workflows in an active-active resiliency mode across multiple regions. The proposed system abstracts the complexities of job and step/task state management, provides automated and customizable retries, and ensures auditability and observability. The system maintains operational continuity and fault tolerance, leveraging lock marker files and a configurable scheduler, even during regional failures. This approach addresses the challenges of asynchronous workflow management, offering enhanced visibility, control, and efficiency in processing batch jobs.
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Afanaseva, I. "TO THE QUESTION OF TEACHING A FUNCTIONAL METHOD FOR SOLVING EQUATIONS AND INEQUALITIES IN THE IMPLEMENTATION OF A SYSTEM-ACTIVITY APPROACH." Scientific heritage, no. 93 (July 22, 2022): 53–55. https://doi.org/10.5281/zenodo.6882457.
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The article introduces the concept of the system-activity approach, describes the system-activity approach and its components main ideas. The conditions of teaching the functional method of solving equations and inequalities of the school mathematics course in the implementation of the system-activity approach are determined.
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Sharma, Aakriti, Anusha Adhikari, Bibek Adhikari, Pratik Pandey, and Indraman Tamrakar. "Active Cell Balancing Approach for Efficient Battery Management System." KEC Journal of Science and Engineering 8, no. 1 (2024): 23–26. http://dx.doi.org/10.3126/kjse.v8i1.69260.
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With the increasing concern for EV and its battery management system, the effective life and performance of li-ion battery is of utmost importance. The battery cells should be frequently equalized in order to increase the lifetime of battery pack. The conventional method of cell balancing is passive cell balancing where excess energy from cell is dissipated in the form of heat until all the cells are equally charged, causing thermal issues and efficiency in the battery pack. This paper proposes a novel approach for improving the efficiency of battery management systems through active cell balancing using the Kalman filter algorithm thereby overcoming the drawbacks of passive cell balancing. The goal of this paper is to develop a system that can extend the lifespan of batteries by ensuring that each cell is charged and discharged evenly. The proposed system includes an active balancing circuit that uses the Kalman filter algorithm to estimate the state of each battery cell and determine the optimal charging and discharging currents.
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Altinoz, O. Tolga. "Multiobjective PID controller design for active suspension system: scalarization approach." An International Journal of Optimization and Control: Theories & Applications (IJOCTA) 8, no. 2 (2018): 183–94. http://dx.doi.org/10.11121/ijocta.01.2018.00399.
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In this study, the PID tuning method (controller design scheme) is proposed for a linear quarter model of active suspension system installed on the vehicles. The PID tuning scheme is considered as a multiobjective problem which is solved by converting this multiobjective problem into single objective problem with the aid of scalarization approaches. In the study, three different scalarization approaches are used and compared to each other. These approaches are called linear scalarization (weighted sum), epsilon-constraint and Benson’s methods. The objectives of multiobjective optimization are selected from the time-domain properties of the transient response of the system which are overshoot, rise time, peak time and error (in total there are four objectives). The aim of each objective is to minimize the corresponding property of the time response of the system. First, these four objective is applied to the scalarization functions and then single objective problem is obtained. Finally, these single objective problems are solved with the aid of heuristic optimization algorithms. For this purpose, four optimization algorithms are selected, which are called Particle Swarm Optimization, Differential Evolution, Firefly, and Cultural Algorithms. In total,twelve implementations are evaluated with the same number of iterations. In this study, the aim is to compare the scalarization approaches and optimization algorithm on active suspension control problem. The performance of the corresponding cases (implementations) are numerically and graphically demonstrated on transient responses of the system.
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Rigaud, B., Y. Shi, N. Chauveau, and J. P. Morucci. "Experimental acquisition system for impedance tomography with active electrode approach." Medical & Biological Engineering & Computing 31, no. 6 (1993): 593–99. http://dx.doi.org/10.1007/bf02441807.
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Ahemad, Noormahamad Nadaf*1 Vaibhav Gaikwad2 Dhiraj More1 Mustafa Bashir Mujavar1 Pavanraje Dhokate1. "An Advance Drug Delivery Approach: Osmotic Pump." International Journal of Pharmaceutical Sciences 3, no. 3 (2025): 385–97. https://doi.org/10.5281/zenodo.14989518.
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The past few decades were challenging for the pharmaceutical industry to bring the new active pharmaceutical ingredients APIs in market. Due to the multiple challenges the novel drug delivery approach were increased. From the numerous novel drug delivery systems one of the definitive approach is Osmotic drug delivery system. Osmotic drug delivery system is a type of controlled drug release and brings relief from local gastrointestinal or systemic toxicity. The active drug molecule is present in the core compartment of the osmotic pump. Osmotic pump utilized in oral administration and implantation procedures. It is much easier to develop a novel drug delivery system in comparison to bring a new chemical entity in world. Novel drug delivery approach can increase market value of an old chemical entity by allowing it to increase its lifespan in market and conflicting to other chemical entity.
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Miftakul Amin, M., Andino Maseleno, Shankar K, Eswaran Perumal, R. M Vidhyavathi, and Lakshmanaprabu SK. "Active Database System Approach and Rule Based in the Development of Academic Information System." International Journal of Engineering & Technology 7, no. 2.26 (2018): 95. http://dx.doi.org/10.14419/ijet.v7i2.26.14361.
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Active database system is a database system which is capable of generating a certain action automatically if it detects an event that meets certain conditions. The existence of Event Condition Action (ECA) rules and functional components such as triggers, stored procedures, and stored functions that are owned by active database system make the database system has the ability to automatically monitor input and output data. Separation of ECA rules components in the database with the application program will also facilitate the development of information systems. This research applies active database system in academic information system, so that academic business rules can be planted in database software and be able to produce the right solution automatically. The addition of active database system components to the database software makes procedures such as subject distribution, study plans, academic leave, values, thesis defense and other processes can be monitored by the system automatically. This can be done because between applications and databases use the model driven approach parameters to communicate with each other. The results of this research prove that a database is not only has function as a container of data, but can control the information system actively, this is caused by the logic of programs that are generally planted in the application layer that can be moved and planted in the database software.
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Gawthrop, Peter J., Simon A. Neild, and David J. Wagg. "Semi-active damping using a hybrid control approach." Journal of Intelligent Material Systems and Structures 23, no. 18 (2012): 2103–16. http://dx.doi.org/10.1177/1045389x12436734.
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In this article, a hybrid control framework is used to design semi-active controllers for vibration reduction. It is shown that the semi-active skyhook damper, typically used for vibration reduction, can be recast in the framework of an event-driven intermittent controller. By doing this, we can then exploit the well-developed techniques associated with hybrid control theory to design the semi-active control system. Illustrative simulation examples are based on a 2 degree-of-freedom system, often used to model the dynamics of a quarter car body model. The simulation results demonstrate how hybrid control design techniques can improve the overall performance of the semi-active control system.
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Sijakovic, Nenad, Aleksandar Terzic, Georgios Fotis, et al. "Active System Management Approach for Flexibility Services to the Greek Transmission and Distribution System." Energies 15, no. 17 (2022): 6134. http://dx.doi.org/10.3390/en15176134.
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New methods for state estimation are required due to the complexity of the topology of transmission and distribution systems, and the predictability in the management of prosumer dispatch. This paper describes a pilot project in Greece that, in accordance with OneNet’s architecture, addresses the challenges of congestion and balancing management that system operators face due to the high penetration of renewable energy sources. The respective data requirements and the IT/OT environment are described, as well as the interconnections among the various modules and functionalities. Available resources of the grid’s flexibility are identified, and the implementation of an integrated monitoring system based on efficient forecasting of volatile generation and demand is addressed. Congestion management and frequency and voltage control are in the center of interest of the demonstrator where, in close collaboration with system operators, respective network models are being developed.
Dissertations / Theses on the topic "System-active approach"
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Brunell, Brent Jerome 1972. "A system identification approach to active control of thermoacoustic instabilities." Thesis, Massachusetts Institute of Technology, 2000. http://hdl.handle.net/1721.1/88832.
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Du, S., Wyk BJ Van, G. Qi, and C. Tu. "Chaotic system synchronization with an unknown master model using a hybrid HOD active control approach." Elsevier, 2009. http://encore.tut.ac.za/iii/cpro/DigitalItemViewPage.external?sp=1001363.
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a b s t r a c t
In this paper, a hybrid method using active control and a High Order Differentiator (HOD)
methodology is proposed to synchronize chaotic systems. Compared to some traditional
active control methods, this new method can synchronize chaotic systems where only output
states of the master system are available, i.e. the system is considered a black box. The
HOD is used to estimate the derivative information of the master system followed by an
active control methodology relying on HOD information. The Qi hyperchaotic system is
used to verify the performance of this hybrid method. The proposed method is also compared
to some traditional methods. Experimental results show that the proposed method
has high synchronization precision and speed and is robust against uncertainties in the
master system. The circus implements of the proposed synchronizing scheme are included
in this paper. The simulation results show the feasibility of the proposed scheme.
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Uppströmer, Viktor, and Henning Råberg. "Detecting Lateral Movement in Microsoft Active Directory Log Files : A supervised machine learning approach." Thesis, Blekinge Tekniska Högskola, Institutionen för datavetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-18337.
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Cyberattacker utgör ett stort hot för dagens företag och organisationer, med engenomsnittlig kostnad för ett intrång på ca 3,86 miljoner USD. För att minimera kostnaden av ett intrång är det viktigt att detektera intrånget i ett så tidigt stadium som möjligt. Avancerande långvariga hot (APT) är en sofistikerad cyberattack som har en lång närvaro i offrets nätverk. Efter attackerarens första intrång kommer fokuset av attacken skifta till att få kontroll över så många enheter som möjligt på nätverket. Detta steg kallas för lateral rörelse och är ett av de mest kritiska stegen i en APT. Syftet med denna uppsats är att undersöka hur och hur väl lateral rörelse kan upptäckas med hjälp av en maskininlärningsmetod. I undersökningen jämförs och utvärderas fem maskininlärningsalgoritmer med upprepad korsvalidering följt av statistisk testning för att bestämma vilken av algoritmerna som är bäst. Undersökningen konkluderar även vilka attributer i det undersökta datasetet som är väsentliga för att detektera laterala rörelser. Datasetet kommer från en Active Directory domänkontrollant där datasetets attributer är skapade av korrelerade loggar med hjälp av datornamn, IP-adress och användarnamn. Datasetet består av en syntetisk, samt, en verklig del vilket skapar ett semi-syntetiskt dataset som innehåller ett multiklass klassifierings problem. Experimentet konkluderar att all fem algoritmer klassificerar rätt med en pricksäkerhet (accuracy) på 0.998. Algoritmen RF presterar med den högsta f-measure (0.88) samt recall (0.858), SVM är bäst gällande precision (0.972) och DT har denlägsta inlärningstiden (1237ms). Baserat på resultaten indikerar undersökningenatt algoritmerna RF, SVM och DT presterar bäst i olika scenarier. Till exempel kan SVM användas om en låg mängd falsk positiva larm är viktigt. Om en balanserad prestation av de olika prestanda mätningarna är viktigast ska RF användas. Undersökningen konkluderar även att en stor mängd utav de undersökta attributerna av datasetet kan bortses i framtida experiment, då det inte påverkade prestandan på någon av algoritmerna.<br>Cyber attacks raise a high threat for companies and organisations worldwide. With the cost of a data breach reaching $3.86million on average, the demand is high fora rapid solution to detect cyber attacks as early as possible. Advanced persistent threats (APT) are sophisticated cyber attacks which have long persistence inside the network. During an APT, the attacker will spread its foothold over the network. This stage, which is one of the most critical steps in an APT, is called lateral movement. The purpose of the thesis is to investigate lateral movement detection with a machine learning approach. Five machine learning algorithms are compared using repeated cross-validation followed statistical testing to determine the best performing algorithm and feature importance. Features used for learning the classifiers are extracted from Active Directory log entries that relate to each other, with a similar workstation, IP, or account name. These features are the basis of a semi-synthetic dataset, which consists of a multiclass classification problem. The experiment concludes that all five algorithms perform with an accuracy of 0.998. RF displays the highest f1-score (0.88) and recall (0.858), SVM performs the best with the performance metric precision (0.972), and DT has the lowest computational cost (1237ms). Based on these results, the thesis concludes that the algorithms RF, SVM, and DT perform best in different scenarios. For instance, SVM should be used if a low amount of false positives is favoured. If the general and balanced performance of multiple metrics is preferred, then RF will perform best. The results also conclude that a significant amount of the examined features can be disregarded in future experiments, as they do not impact the performance of either classifier.
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Ourique, de Morais Wagner. "Architecting Smart Home Environments for Healthcare : A Database-Centric Approach." Doctoral thesis, Högskolan i Halmstad, Centrum för forskning om inbyggda system (CERES), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-29227.
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The development of system architectures and applications for smart homes and ambient assisted living has been the main activity of a number of academic and industrial research projects around the world. Existing system architectures for smart environments usually employ different architectural styles in a multi-layer logical architecture to support the integration and interoperation of heterogeneous hardware and software technologies, which are subsequently used to provide two major functionalities: monitoring and assistance. It is also usual among existing architectures that the database management system is the most common but the least exploited architectural component, existing in the periphery of the system and devoted exclusively for data storage and retrieval. However, database technology has advanced and matured considerably over the years, and, as a result, current database management systems can be and do more. This thesis considers the hypothesis of several features of modern database management systems being employed to address functional (e.g. well-being and security monitoring, automated control, data processing) and non-functional (e.g. interoperability, extensibility, data security and privacy) requirements of smart environments, i.e. the database management system serves as a platform for smart environments. The scope of this thesis is therefore to investigate the possibility of using different features supported by database management systems to create a database-centric system architecture for the development of smart home environments and ambient assisted living. The thesis also investigates the development of applications for health monitoring and assistance: 1) a serious game for fall prevention that assists people in practicing Tai Chi at home, and 2) a non-intrusive home-based method for sleep assessment. These features are explored in this thesis to address general functional aspects of smart environments, such as monitoring, processing, coordination and control of various types of events in a given environment. Extensibility and security features and cross-platform capabilities of database management systems are employed to accommodate non-functional, but still technical, properties of smart environments, including interoperability, extensibility, portability, scalability, security and privacy. Heterogeneous technologies are integrated into the system using programming language and platform independent software resource adapters. Interoperation among integrated technologies is mediated in an active database. The feasibility of the proposed database-centric system architecture was pragmatically investigated with the development of a "smart bedroom'' demonstrator and with the implementation of a number of short-term and long-term types of services to support active aging, aging in place and ambient assisted living. In the proposed architecture, active in-database processing maintains sensitive data within the database. This increases data security and independence from external software applications for data analysis. Changes in the system are managed during runtime, which improves flexibility and avoids system downtime. The proposed system architecture was evaluated taking into account different application scenarios and heterogeneous computing platforms. As a conclusion, modern database management systems support features that can be successfully employed in a database-centric system architecture to effectively and efficiently address functional and non-functional requirements of smart environments.
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Jan, Taimoor. "Active fault tolerant control for general aircraft using NLGA." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2018.
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The aerospace applications are considered among one of most complex. The non-linear dynamics and coupling within aircraft systems remains a challenging research area for long time till date. The faults and failures are ordinary in all electrical/mechanical sub-systems. The probability of occurrence can be debatable but it is impossible to assume fault free systems. The faults in input actuators in aircraft can be fatal and highly undesirable. There is a need to make aircraft remain safe/operational and stable in case of such undesirable event. This goal can only be achieved using fault tolerant controls FTC, and in this case active fault tolerant control system AFTCS methodology is considered and investigated. The fault detection and diagnosis FDD can provide the basis of AFTCS. The accurate and effective FDD is explored using Non-linear Geometric Approach (NLGA) for non-linear dynamics of aircraft. In addition, the criticality of descent/landing phases is not unknown since accidents are more prone to happen during these phases. The backstepping (BS) control scheme is proposed as a non-linear method to achieve glide slope control system. The proposed control scheme provided sound tracking performance. The FDD module in combination with BS based control system, provided AFTCS which is capable of stabilizing the aircraft and achieving the desired performance in both scenarios, i.e. faulty/faultless. Minor performance degradation is present but comfort/workload of pilot remains similar to faultless system since aircraft recovers nominal condition promptly, post-fault occurrence. The robustness analysis is satisfactory.
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Chen, Yinlin. "A High-quality Digital Library Supporting Computing Education: The Ensemble Approach." Diss., Virginia Tech, 2017. http://hdl.handle.net/10919/78750.
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Educational Digital Libraries (DLs) are complex information systems which are designed to support individuals' information needs and information seeking behavior. To have a broad impact on the communities in education and to serve for a long period, DLs need to structure and organize the resources in a way that facilitates the dissemination and the reuse of resources. Such a digital library should meet defined quality dimensions in the 5S (Societies, Scenarios, Spaces, Structures, Streams) framework - including completeness, consistency, efficiency, extensibility, and reliability - to ensure that a good quality DL is built.
In this research, we addressed both external and internal quality aspects of DLs. For internal qualities, we focused on completeness and consistency of the collection, catalog, and repository. We developed an application pipeline to acquire user-generated computing-related resources from YouTube and SlideShare for an educational DL. We applied machine learning techniques to transfer what we learned from the ACM Digital Library dataset. We built classifiers to catalog resources according to the ACM Computing Classification System from the two new domains that were evaluated using Amazon Mechanical Turk. For external qualities, we focused on efficiency, scalability, and reliability in DL services. We proposed cloud-based designs and applications to ensure and improve these qualities in DL services using cloud computing. The experimental results show that our proposed methods are promising for enhancing and enriching an educational digital library.
This work received support from ACM, as well as the National Science Foundation under Grant Numbers DUE-0836940, DUE-0937863, and DUE-0840719, and IMLS LG-71-16-0037-16.<br>Ph. D.
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Mousavinejad, Seyed Eman. "Advanced Terminal Sliding Mode Control Approach to Integrated Steer-by-Wire and Differential Braking of Ground Vehicles." Thesis, Griffith University, 2016. http://hdl.handle.net/10072/367350.
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Advances in electronic technology have had a profound impact on the design and development of modern vehicle systems. These advances have provided the basis for a research into active vehicle control paradigm with the aim of improving vehicle safety and its dynamic performance.
The vehicle stability control (VSC) concept has recently been born in response to such a research challenge. Its refinement is looking for an algorithm able of integrating vehicle dynamics control (IVDC) by way of coordinating the active front steering (AFS) system and direct yaw-moment control (DYC) system. Thus, when the vehicle is in the normal driving situation, the AFS system is involved for handling enhancement; however, as the vehicle reaches the handling limits, both the AFS and DYC systems are integrated to ensure the vehicle stability.
Recent research into VSC has indicated that the IVDC concept may be able to enhance the handling and stability of ground vehicle by controlling the main two control objectives, yaw rate and body sideslip angle, effectively in severe cornering manoeuvres and the steady-state condition, and several control strategies have been investigated to design an IVDC system. However, most of these control methods have not been focused on improving the transient response for vehicle yaw rate and sideslip angle tracking controllers in the presence of vehicle dynamics uncertainties and external disturbance. Therefore, further improvement of the transient response for the tracking controllers is still the most needed research topics.<br>Thesis (Masters)<br>Master of Philosophy (MPhil)<br>Griffith School of Engineering<br>Full Text
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OMONG, Mark Donald. "Enhancing Collaborative Governance to Build Active Citizenship through Social Inclusion in Historical Neighborhood Regeneration: A Dynamic Performance Governance Approach." Doctoral thesis, Università degli Studi di Palermo, 2021. http://hdl.handle.net/10447/515030.
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Active citizenship and collaborative governance can be supported to enhance urban regeneration and social inclusion through Dynamic Performance Governance (DPG). The problem of urban decay and urban decline is identified as the main problem that continues to affect cities and urban areas. The study focuses on the urban regeneration experience of the historic urban neighborhood of Ballaro which is located in the historic city of Palermo. Urban regeneration is essential for transforming urban neighborhoods which are at risk of decline and blight. However, the lack of a social aspect such as social inclusion through active citizenship was identified to affect the sustainability of urban regeneration policies. This study therefore illustrates how social inclusion as a social aspect of urban regeneration can be attained through active citizenship. It also illustrates how active citizenship can be supported by collaborative governance so as to generate community outcomes. The main goal of the study is to demonstrate how DPG can be applied by decision makers to design and implement sustainable urban regeneration policies. In this regard, urban regeneration was depicted as an intervention within an interconnected system, rather than a sum of single interventions. The study therefore identifies social inclusion as a key variable which can be promoted through active citizenship and collaborative governance so as to ensure sustainable urban regeneration. The following objectives were studied: (1) To identify factors which influence the growth and decline of cities and urban neighborhoods. (2) To illustrate how active citizenship can be generated so as to foster collaborative governance. (3) To determine how stakeholder collaboration can be used to generate sustainable outcomes. (4) To demonstrate how DPG can be applied in local areas strategic planning to support sustainable outcomes. Findings from the study indicate that factors associated with urban decline include the presence of social ‘wicked’ problems and dynamic complexity. The factors that support urban growth include, the balanced scorecard for strategic planning, social inclusion, system dynamics, and implementation of urban regeneration policies. Active citizenship was found to sustain collaborative governance. Collaborative governance across stakeholder groups was found to be essential in fostering sustainable outcomes. The study illustrates the role of DPG in decision making and strategic planning. In addition, DPG was used as a methodology in the study. The study recommends a theoretical and practical integration of Dynamic Performance Management and Governance in urban settings which will enrich this field of study, and improve the way decision makers approach urban regeneration.<br>Active citizenship and collaborative governance can be supported to enhance urban regeneration and social inclusion through Dynamic Performance Governance (DPG). The problem of urban decay and urban decline is identified as the main problem that continues to affect cities and urban areas. The study focuses on the urban regeneration experience of the historic urban neighborhood of Ballaro which is located in the historic city of Palermo. Urban regeneration is essential for transforming urban neighborhoods which are at risk of decline and blight. However, the lack of a social aspect such as social inclusion through active citizenship was identified to affect the sustainability of urban regeneration policies. This study therefore illustrates how social inclusion as a social aspect of urban regeneration can be attained through active citizenship. It also illustrates how active citizenship can be supported by collaborative governance so as to generate community outcomes. The main goal of the study is to demonstrate how DPG can be applied by decision makers to design and implement sustainable urban regeneration policies. In this regard, urban regeneration was depicted as an intervention within an interconnected system, rather than a sum of single interventions. The study therefore identifies social inclusion as a key variable which can be promoted through active citizenship and collaborative governance so as to ensure sustainable urban regeneration. The following objectives were studied: (1) To identify factors which influence the growth and decline of cities and urban neighborhoods. (2) To illustrate how active citizenship can be generated so as to foster collaborative governance. (3) To determine how stakeholder collaboration can be used to generate sustainable outcomes. (4) To demonstrate how DPG can be applied in local areas strategic planning to support sustainable outcomes. Findings from the study indicate that factors associated with urban decline include the presence of social ‘wicked’ problems and dynamic complexity. The factors that support urban growth include, the balanced scorecard for strategic planning, social inclusion, system dynamics, and implementation of urban regeneration policies. Active citizenship was found to sustain collaborative governance. Collaborative governance across stakeholder groups was found to be essential in fostering sustainable outcomes. The study illustrates the role of DPG in decision making and strategic planning. In addition, DPG was used as a methodology in the study. The study recommends a theoretical and practical integration of Dynamic Performance Management and Governance in urban settings which will enrich this field of study, and improve the way decision makers approach urban regeneration.
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Moscoso, Manolete S. "The health psychology: a multidisciplinary approach about stress and behavioral change." Pontificia Universidad Católica del Perú, 2013. http://repositorio.pucp.edu.pe/index/handle/123456789/102061.
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This article reviews research and professional trends in the field of health psychology and behavioral medicine. I also discuss recent research on stress, immune system, behavioral change and health promotion. An increasing focus on the human immunodeficiencyvirus (HN/AIDS) is very important given the Rde of health psychology in terms of prevencing further spread of the epidemic and in maintaining a good qualiry of life. I examined psychosocial and immune system stress responses in individuals before and after their nocification of HN status andas a consequence of stress management intervention procedures. Psychological and social factors in the development of illness are considered.<br>Este articulo revisa líneas de investigación y profesionales en el campo de la psicología de la salud y la medicina conductual. Se discuten investigaciones recientes acerca del estrés, el sistema inmunológico, los cambios conducruales y la promoción de la salud. Un énfasis en el virus de inmunodeficiencia (HN/SIDA) es muy importante dado el rol de la psicología de la salud para prevenir la expansión de la epidemia y mantener una buena calidad de vida. Se han examinado las respuestas psicosociales y del sistema inmunológico de individuos antes y después de ser notificados de su status de HN y como consecuencia de los procedimientos de intervención para manejar el estrés. Se consideran los factores psicológicos y sociológicos en el desarrollo de la enfermedad.
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Bayoudh, Mehdi. "Active Diagnosis of Hybrid Systems Guided by Diagnosability Properties - Application to Autonomous Satellites." Thesis, Toulouse, INPT, 2009. http://www.theses.fr/2009INPT069H.
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Motivée par les besoins du domaine spatial en termes de diagnostic embarqué et d’autonomie, cette thèse s’intéresse aux problèmes de diagnostic, de diagnosticabilité et de diagnostic actif des systèmes hybrides. Un formalisme hybride est proposé pour représenter les deux dynamiques, continues et discrètes, du système. En s’appuyant sur ce modèle, une approche de diagnostic passif est proposée en mariant les techniques des systèmes à événements discrets et des systèmes continus. Un cadre formel pour la diagnosticabilité des systèmes hybrides a également été établi proposant des définitions et des critères pour la diagnosticabilité hybride. Suite à un diagnostic passif ambigu, le diagnostic actif est nécessaire afin de désambiguïser l’état du système. Cette thèse propose donc une approche de diagnostic actif, qui partant d’un état de croyance incertain, fait appel aux propriétés de diagnosticabilité du système pour déterminer la configuration où les fautes peuvent être discriminées. Une nouvelle machine à états finis appelée diagnostiqueur actif est introduite permettant de formaliser le diagnostic actif comme un problème de planification conditionnelle. Un algorithme d’exploration de graphes ET-OU est proposé pour calculer les plans de diagnostic actif. Finalement, l’approche de diagnostic a été testée sur le Système de Contrôle d’Attitude (SCA) d’un satellite de Thales Alenia Space. Le module de diagnostic a été intégré dans la boucle fermée de commande. Des scénarios de faute ont été testés donnant des résultats très satisfaisants<br>Motivated by the requirements of the space domain in terms of on-board diagnosis and autonomy, this thesis addresses the problems of diagnosis, diagnosability and active diagnosis of hybrid systems. Supported by a hybrid modeling framework, a passive approach for model-based diagnosis mixing discrete-event and continuous techniques is proposed. The same hybrid model is used to define the diagnosability property for hybrid systems and diagnosability criteria are derived. When the diagnosis provided by the passive diagnosis approach is ambiguous, active diagnosis is needed. This work provides a method for performing such active diagnosis. Starting with an ambiguous belief state, the method calls for diagnosability analysis results to determine a new system configuration in which fault candidates can be discriminated. Based on a new finite state machine called the diagnoser, the active diagnosis is formulated as a conditional planning problem and an AND-OR graph exploration algorithm is proposed to determine active diagnosis plans. Finally, the diagnosis approach is tested on the Attitude Control System (ACS) of a satellite simulator provided by Thales Alenia Space. The diagnosis module is successfully tested on several fault scenarios and the obtained results are reported
Books on the topic "System-active approach"
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Yukio, Yamori, and Lenfant Claude, eds. Prevention of cardiovascular diseases: An approach to active long life : proceedings of the International Symposium on the Prevention of Cardiovascular Diseases--an Approach to a Health Long Life, Tokyo, Japan, October 26, 1985. Excerpta Medica, 1987.
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Kleandrov, Mihail. Justice and equity. INFRA-M Academic Publishing LLC., 2021. http://dx.doi.org/10.12737/1816287.
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The monograph examines the fundamental problems of justice from the point of view of the need to ensure organizational and legal means of justice in judicial activity. The problems of philosophical, legal and other approaches to understanding justice and the mechanism of justice are studied; the problems of justice in the system of current legislation, including in the conditions of uncertainty of legal norms, within the framework of judicial discretion, in judicial law - making and rule — making; the problems of evidence in court proceedings; the problems of fair justice in extreme conditions of the coronavirus pandemic; as a vector of the future-the problems of justice carried out by artificial intelligence; the problem of justice of the death sentence. Proposals aimed at improving the mechanism of fair justice are being made. 
 For employees of legislative, judicial and law enforcement agencies, active judges and those who want to become them, scientists, teachers, graduate students and law students, as well as for practicing lawyers.
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Grineva, Elizaveta, Larisa Davletshina, and Nadezhda Bibikova. Formation of ecological worldview of schoolchildren. INFRA-M Academic Publishing LLC., 2023. http://dx.doi.org/10.12737/1882578.
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The textbook outlines the theoretical foundations of the formation of an ecological worldview, provides a model of this process, reveals modern approaches and ideological aspects of environmental education. A conceptual analysis of creativity in the structure of the general abilities of the individual is proposed, innovative technologies in environmental education that contribute to the development of creativity are considered, and attention is also paid to socio-ecological design. The issues of diagnostics of the level of formation of the main indicators of the ecological worldview of students are considered. The manual includes a system of workshops on the development of creativity in the process of environmental education and socio-ecological design, educational tasks in the unity of intellectual, emotional-value and practical-active components, in the unity of global, national and local history components and in the unity of natural science, humanities and cultural components.
 Meets the requirements of the federal state educational standards of higher education of the latest generation.
 For undergraduate students studying in the areas of training "Preschool education", "Psychological and pedagogical education", "Pedagogical education", "Social work", as well as students of secondary vocational education institutions studying in the specialty "Teaching in primary classes".
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Durakova, Irina, Larisa Matasova, Ekaterina Mayer, et al. Personnel management in Russia: reboot. Book 11. INFRA-M Academic Publishing LLC., 2023. http://dx.doi.org/10.12737/2035498.
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The monograph contains the results of research concerning, firstly, new ideas about employees and about human resource management in the context of a reset of the labor market and sanctions restrictions. In the context of these directions, the competition factor is considered as an imperative to reset the supply and demand of labor, the specifics of the modern psyche and existential reality, the role of human capital in the sustainable development of the corporation. Secondly, the actual role of continuing education as a basis for the competitiveness of workers in a period of economic instability, its inclusive scenario and, as a consequence, knowledge, formed, including taking into account the policy of digitalization. Thirdly, issues of well—being, the content of which is a culture of caring and psychological safety of employees, including conditions of crisis economic development, an inclusive environment, family-friendly policy of the organization. Fourth, the problems of managing older workers in the concepts and practice of personnel management: professional success in aging societies, technology for achieving active longevity, training, productivity and remuneration. Fifth, resetting approaches to personnel management: the formation of an organization's culture in the context of the development of information and communication technologies, the development of a system of documentation support for personnel management in order to reduce personnel and legal risks.
 For students, postgraduates and teachers of economic universities and faculties, as well as a wide range of readers interested in personnel management issues.
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Cottrell, David M. Mastering the Financial Accounting Research System On-Line: An Active Learning Approach. Wiley & Sons, Incorporated, John, 2005.
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Experiencing Business: An Active Learning Approach (Applies Baldrige Performance Excellence System, Updated Edition for 2004). 2nd ed. Fountainhead Press, 2003.
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Treptow, Nancy Anne. A genetic approach to the study of protein-protein interactions in an active transport system of Escherichia coli. 1987.
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Marakas, George. Systems Analysis & Design: An Active Approach. McGraw-Hill/Irwin, 2004.
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Marakas, George M. Systems Analysis and Deisgn: An Active Approach. Prentice Hall, 2000.
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Marakas, George M. Systems Analysis and Deisgn: An Active Approach. Prentice Hall, 2000.
Find full textBook chapters on the topic "System-active approach"
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Schagaev, Igor, and Brian Robinson Kirk. "Active System Control and Safety Approach, and Regulation in Other Application Domains." In Active System Control. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-46813-6_2.
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Llamosi, Artémis, Adel Mezine, Florence d’Alché-Buc, Véronique Letort, and Michèle Sebag. "Experimental Design in Dynamical System Identification: A Bandit-Based Active Learning Approach." In Machine Learning and Knowledge Discovery in Databases. Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-662-44851-9_20.
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Dittrich, Angelika Kotz. "Adding Active Functionality to an Object-Oriented Database System — a Layered Approach." In Informatik aktuell. Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-86096-6_4.
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Wei, Wei, XuDong Shen, YanJie Shao, and Min Zuo. "Linear Active Disturbance Rejection Control for Nanopositioning System by ITAE Optimal Tuning Approach." In Lecture Notes in Electrical Engineering. Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6499-9_31.
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Abdul-jabbar, Thealfaqar A., Adel A. Obed, and Ahmed J. Abid. "An Approach for an Intelligent Lithium-Ion Battery Management System with Active Balancing." In Lecture Notes in Electrical Engineering. Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-16-9488-2_71.
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Wei, Wei, Weicun Zhang, Donghai Li, and Yuqiong Zhang. "On the Stability of Linear Active Disturbance Rejection Control: Virtual Equivalent System Approach." In Proceedings of the 2015 Chinese Intelligent Systems Conference. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-48365-7_31.
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Spong, Chris, Mike Gibb, Javaria Waqar, et al. "Active Travel Infrastructure (ATI) in Ireland – An Asset Management Approach." In Lecture Notes in Mobility. Springer Nature Switzerland, 2025. https://doi.org/10.1007/978-3-031-89444-2_53.
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Abstract Ireland is committed to boosting active travel as a key part of its decarbonisation plan. The development of Active Travel Infrastructure (ATI) which facilitates walking, cycling, and other non-motorised forms of transportation, is a focal area of Ireland's substantial capital investment efforts aimed at advancing its sustainable transportation goals. While investing in the new ATI, the aspect of ongoing maintenance and renewal is frequently overlooked. However, to ensure the realisation of the benefits of active travel, it is essential to have a sustainable asset management system accompanied by sufficient funding to uphold a consistent level of service throughout the infrastructure’s lifespan. This paper highlights the importance of a systematic management, maintenance, and renewal strategy for ATI assets that includes planned asset renewal, routine maintenance activities, and winter operations. It also proposes the funding requirements for maintaining and preserving the lifespan of existing and new ATI.
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Trabelsi, Hassen, Pierre-Alain Yvars, Jamel Louati, and Mohamed Haddar. "Application of Set-based-approach for the Global Sizing of an Active Macpherson Suspension System." In Design and Modeling of Mechanical Systems—III. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-66697-6_106.
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Awad, Safa, Rui Wang, and Ismael Soto. "Target Detection in ISAC System Equipped with IRS: A Joint Active and Passive Beamforming Approach." In Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering. Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-67162-3_5.
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Yamamoto, Noriyasu. "An Integrated Online Learning Approach Using a Smartphone-Based Active Learning System and a Web Video On-Demand System." In Advances in Intelligent Systems and Computing. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-57811-4_35.
Full textConference papers on the topic "System-active approach"
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Naufal, Ahmad Fikri, Andris Yanuar Mifdar Munandar, Harry Freddy Handrian, Brahmantya Aji Pramudita, and Bandiyah Sri Aprillia. "Simulation of Active Balancing Approach Utilizing Capacitor-Based Topology for Battery Management System." In 2024 IEEE International Conference on Smart Mechatronics (ICSMech). IEEE, 2024. https://doi.org/10.1109/icsmech62936.2024.10812312.
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Lv, Lingling, Bo Hou, Shijie Zhang, and Quanzhen Huang. "Active Disturbance Rejection Control of Satellite Attitude Based on High-Order Fully Actuated System Approach." In 2024 IEEE 13th Data Driven Control and Learning Systems Conference (DDCLS). IEEE, 2024. http://dx.doi.org/10.1109/ddcls61622.2024.10606888.
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Zhu, Guanrong, Wenbo Luo, Jianan Yan, Guangzong Huang, and Ziwei Fan. "A study on active power balance control of wind/photovoltaic storage system based on multiple rotating vector superposition approach." In 2024 2nd International Conference on Signal Processing and Intelligent Computing (SPIC). IEEE, 2024. http://dx.doi.org/10.1109/spic62469.2024.10691507.
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Schipani, P., F. Perrotta, and L. Marty. "Integrated modeling approach for an active optics system." In SPIE Astronomical Telescopes + Instrumentation, edited by Martin J. Cullum and George Z. Angeli. SPIE, 2006. http://dx.doi.org/10.1117/12.670528.
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Basturk, Halil Ibrahim. "A backstepping approach for an active suspension system." In 2016 American Control Conference (ACC). IEEE, 2016. http://dx.doi.org/10.1109/acc.2016.7526870.
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Lu, Peng, and Erik-Jan Van Kampen. "Active Fault-Tolerant Control System using Incremental Backstepping Approach." In AIAA Guidance, Navigation, and Control Conference. American Institute of Aeronautics and Astronautics, 2015. http://dx.doi.org/10.2514/6.2015-1312.
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Wei, Wei, Han Gao, Tao Xu, et al. "Active distribution network sustainability assessment: A system dynamic approach." In 2017 IEEE Power & Energy Society Innovative Smart Grid Technologies Conference (ISGT). IEEE, 2017. http://dx.doi.org/10.1109/isgt.2017.8086002.
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"Active Database System Realized by a Petri Net Approach." In The 6th International Workshop on Modelling, Simulation,Verification and Validation of Enterprise Information Systems. SciTePress - Science and and Technology Publications, 2008. http://dx.doi.org/10.5220/0001730501500159.
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Bai, Xiaoyin, Yen-Kheng Tan, Zhi-Hui Kong, and Siek Liter. "Novel active tuning approach for resonant-mode wireless charging system." In 2014 IEEE International Conference on Industrial Technology (ICIT). IEEE, 2014. http://dx.doi.org/10.1109/icit.2014.6894890.
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Gaskill, A. "A case study of delivering an Active Network Management control system on a UK DNO network." In IET Conference on Power in Unity: a Whole System Approach. Institution of Engineering and Technology, 2013. http://dx.doi.org/10.1049/ic.2013.0126.
Full textReports on the topic "System-active approach"
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Morkun, Volodymyr S., Сергій Олексійович Семеріков, and Svitlana M. Hryshchenko. Use of the system Moodle in the formation of ecological competence of future engineers with the use of geoinformation technologies. Видавництво “CSITA”, 2016. http://dx.doi.org/10.31812/0564/718.
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At present the information and communication technologies in education can be a catalyst in solving important social problems connected with increasing the educational resources and services availability and quality, real and equal opportunities in getting education for citizens despite their residence, social status and income. One of the most important education tasks is to develop students’ active cognitive attitude to knowledge. Cognitive activity in universities is a necessary stage in preparing for further professional life. The solution of task of formation of ecological competence of mining profile engineer requires the reasonable selection of the means of information and communication technologies conducing formation of ecological competence. Pressing task is constructive and research approach to preparation of future engineers to performance of professional duties in order to make them capable to develop engineering projects independently and exercise control competently. The relevance of the material covered in the article, due to the need to ensure the effectiveness of the educational process in the preparation of the future Mining Engineers. We analyze the source with problems of formation of ecological competence. The article focuses mainly general-purpose computer system support learning Moodle, which allows you to organize individual and collective work of students to master the specialized course teaching material used in teaching special course "Environmental Geoinformatics" in the implementation of educational research.
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Douglas, D. G., R. F. Wise, J. W. Starr, and J. W. Maresca, Jr. Leak testing plan for the Oak Ridge National Laboratory liquid low- level waste system (active tanks). Volume 1, Regulatory background and plan approach; Volume 2, Methods, protocols, and schedules; Volume 3, Evaluation of the ORNL/LT-823DP differential pressure leak detection method. Office of Scientific and Technical Information (OSTI), 1992. http://dx.doi.org/10.2172/7296416.
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Short, Samuel, Bernhard Strauss, and Pantea Lotfian. Emerging technologies that will impact on the UK Food System. Food Standards Agency, 2021. http://dx.doi.org/10.46756/sci.fsa.srf852.
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Rapid technological innovation is reshaping the UK food system in many ways. FSA needs to stay abreast of these changes and develop regulatory responses to ensure novel technologies do not compromise food safety and public health. This report presents a rapid evidence assessment of the emerging technologies considered most likely to have a material impact on the UK food system and food safety over the coming decade. Six technology fields were identified and their implications for industry, consumers, food safety and the regulatory framework explored. These fields are: Food Production and Processing (indoor farming, 3D food printing, food side and byproduct use, novel non-thermal processing, and novel pesticides); Novel Sources of Protein, such as insects (for human consumption, and animal feedstock); Synthetic Biology (including lab-grown meat and proteins); Genomics Applications along the value chain (for food safety applications, and personal “nutrigenomics”); Novel Packaging (active, smart, biodegradable, edible, and reusable solutions); and, Digital Technologies in the food sector (supporting analysis, decision making and traceability). The report identifies priority areas for regulatory engagement, and three major areas of emerging technology that are likely to have broad impact across the entire food industry. These areas are synthetic biology, novel food packaging technologies, and digital technologies. FSA will need to take a proactive approach to regulation, based on frequent monitoring and rapid feedback, to manage the challenges these technologies present, and balance increasing technological push and commercial pressures with broader human health and sustainability requirements. It is recommended FSA consider expanding in-house expertise and long-term ties with experts in relevant fields to support policymaking. Recognising the convergence of increasingly sophisticated science and technology applications, alongside wider systemic risks to the environment, human health and society, it is recommended that FSA adopt a complex systems perspective to future food safety regulation, including its wider impact on public health. Finally, the increasing pace of technological
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Rojas, Eduardo. The Long Road to Housing Reform: Lessons from the Chilean Experience. Inter-American Development Bank, 1999. http://dx.doi.org/10.18235/0008522.
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Chile's housing policy is widely regarded as a success. For almost a decade, new construction has been above the level required to provide houses for new families and replace obsolete structures. This raises hopes that within the next decade all families in Chile will occupy dwellings that comply with minimum quality and service standards. This is no small accomplishment for a country just entering the middle ground in the development scale. Moreover, the private sector is taking an increasingly active role in housing production and financing. This too is a significant achievement considering that in the 1970s most housing was built and financed by the government. Further, government assistance is effectively reaching the poor, and most public resources are benefiting low-income households. Confidence in the housing policy is high among the low-income population as expressed in their high level of participation in a housing savings program and by the absence of land invasions. These accomplishments are the result of a long maturation process in the Chilean housing sector. Fifty years of government policy have consolidated the legal, institutional, and entrepreneurial foundations of the current housing production and financing system. Several success factors can be identified: an integrated sector approach (which incorporates the housing needs of all income groups); an efficient subsidy system (the result of a long process of experimentation); and reforms of the general banking system and the social security system (which created strong institutions to intermediate the financial resources accumulated by pension funds and life insurance companies). Even with its accomplishments, Chile's housing sector still faces significant challenges. Improvements are needed to more effectively mobilize the resources devoted to housing. The lack of coordination between housing and urban development policies is becoming a major liability for both efficient housing production and equitable urban growth. Direct government involvement in house construction and home financing enforce uniformity in design and repayment schedules failing to fully meet the diversified demand of the target households and to fully mobilize the repayment capacity of beneficiaries. It is suggested that it may be time for housing policy to move beyond this basic approach introducing more flexibility through greater market participation in low-income housing.
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Cytryn, Eddie, Mark R. Liles, and Omer Frenkel. Mining multidrug-resistant desert soil bacteria for biocontrol activity and biologically-active compounds. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7598174.bard.
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Control of agro-associated pathogens is becoming increasingly difficult due to increased resistance and mounting restrictions on chemical pesticides and antibiotics. Likewise, in veterinary and human environments, there is increasing resistance of pathogens to currently available antibiotics requiring discovery of novel antibiotic compounds. These drawbacks necessitate discovery and application of microorganisms that can be used as biocontrol agents (BCAs) and the isolation of novel biologically-active compounds. This highly-synergistic one year project implemented an innovative pipeline aimed at detecting BCAs and associated biologically-active compounds, which included: (A) isolation of multidrug-resistant desert soil bacteria and root-associated bacteria from medicinal plants; (B) invitro screening of bacterial isolates against known plant, animal and human pathogens; (C) nextgeneration sequencing of isolates that displayed antagonistic activity against at least one of the model pathogens and (D) in-planta screening of promising BCAs in a model bean-Sclerotiumrolfsii system. The BCA genome data were examined for presence of: i) secondary metabolite encoding genes potentially linked to the anti-pathogenic activity of the isolates; and ii) rhizosphere competence-associated genes, associated with the capacity of microorganisms to successfully inhabit plant roots, and a prerequisite for the success of a soil amended BCA. Altogether, 56 phylogenetically-diverse isolates with bioactivity against bacterial, oomycete and fungal plant pathogens were identified. These strains were sent to Auburn University where bioassays against a panel of animal and human pathogens (including multi-drug resistant pathogenic strains such as A. baumannii 3806) were conducted. Nineteen isolates that showed substantial antagonistic activity against at least one of the screened pathogens were sequenced, assembled and subjected to bioinformatics analyses aimed at identifying secondary metabolite-encoding and rhizosphere competence-associated genes. The genome size of the bacteria ranged from 3.77 to 9.85 Mbp. All of the genomes were characterized by a plethora of secondary metabolite encoding genes including non-ribosomal peptide synthase, polyketidesynthases, lantipeptides, bacteriocins, terpenes and siderophores. While some of these genes were highly similar to documented genes, many were unique and therefore may encode for novel antagonistic compounds. Comparative genomic analysis of root-associated isolates with similar strains not isolated from root environments revealed genes encoding for several rhizospherecompetence- associated traits including urea utilization, chitin degradation, plant cell polymerdegradation, biofilm formation, mechanisms for iron, phosphorus and sulfur acquisition and antibiotic resistance. Our labs are currently writing a continuation of this feasibility study that proposes a unique pipeline for the detection of BCAs and biopesticides that can be used against phytopathogens. It will combine i) metabolomic screening of strains from our collection that contain unique secondary metabolite-encoding genes, in order to isolate novel antimicrobial compounds; ii) model plant-based experiments to assess the antagonistic capacities of selected BCAs toward selected phytopathogens; and iii) an innovative next-generation-sequencing based method to monitor the relative abundance and distribution of selected BCAs in field experiments in order to assess their persistence in natural agro-environments. We believe that this integrated approach will enable development of novel strains and compounds that can be used in large-scale operations.
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Weil, Clifford F., Anne B. Britt, and Avraham Levy. Nonhomologous DNA End-Joining in Plants: Genes and Mechanisms. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7585194.bard.
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Repair of DNA breaks is an essential function in plant cells as well as a crucial step in addition of modified DNA to plant cells. In addition, our inability to introduce modified DNA to its appropriate locus in the plant genome remains an important hurdle in genetically engineering crop species.We have taken a combined forward and reverse genetics approach to examining DNA double strand break repair in plants, focusing primarily on nonhomologous DNA end-joining. The forward approach utilizes a gamma-plantlet assay (miniature plants that are metabolically active but do not undergo cell division, due to cell cycle arrest) and has resulted in identification of five Arabidopsis mutants, including a new one defective in the homolog of the yeast RAD10 gene. The reverse genetics approach has identified knockouts of the Arabidopsis homologs for Ku80, DNA ligase 4 and Rad54 (one gene in what proves to be a gene family involved in DNA repair as well as chromatin remodeling and gene silencing)). All these mutants have phenotypic defects in DNA repair but are otherwise healthy and fertile. Additional PCR based screens are in progress to find knockouts of Ku70, Rad50, and Mre11, among others. Two DNA end-joining assays have been developed to further our screens and our ability to test candidate genes. One of these involves recovering linearized plasmids that have been added to and then rejoined in plant cells; plasmids are either recovered directly or transformed into E. coli and recovered. The products recovered from various mutant lines are then compared. The other assay involves using plant transposon excision to create DNA breaks in yeast cells and then uses the yeast cell as a system to examine those genes involved in the repair and to screen plant genes that might be involved as well. This award supported three graduate students, one in Israel and two in the U.S., as well as a technician in the U.S., and is ultimately expected to result directly in five publications and one Masters thesis.
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Gordon, Dalia, Ke Dong, and Michael Gurevitz. Unexpected Specificity of a Sea Anemone Small Toxin for Insect Na-channels and its Synergic Effects with Various Insecticidal Ligands: A New Model to Mimic. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7697114.bard.
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Motivated by the high risks to the environment and human health imposed by the current overuse of chemical insecticides we offer an alternative approach for the design of highly active insect-selective compounds that will be based on the ability of natural toxins to differentiate between insect and mammalian targets. We wish to unravel the interacting surfaces of insect selective toxins with their receptor sites on voltage-gated sodium channels. In this proposal we put forward two recent observations that may expedite the development of a new generation of insect killers that mimic the highly selective insecticidal toxins: (i) A small (27aa) highly insecticidal sea anemone toxin, Av3, whose toxicity to mammals is negligible; (ii) The prominent positive cooperativity between distinct channel ligands, such as the strong enhancement of pyrethroids effects by anti-insect selective scorpion depressant toxins. We possess a repertoire of insecticidal toxins and sodium channel subtypes all available in recombinant form for mutagenesis followed by analysis of various pharmacological, electrophysiological, and structural methods. Our recent success to express Av3 provides for the first time a selective toxin for receptor site-3 on insect sodium channels. In parallel, our recent success to determine the structures and bioactive surfaces of insecticidal site-3 and site-4 toxins establishes a suitable system for elucidation of toxin-receptor interacting faces. This is corroborated by our recent identification of channel residues involved with these two receptor sites. Our specific aims in this proposal are to (i) Determine the bioactive surface of Av3 toward insect Na-channels; (ii) Identify channel residues involved in binding or activity of the insecticidal toxins Av3 and LqhaIT, which differ substantially in their potency on mammals; (iii) Illuminate channel residues involved in recognition by the anti-insect depressant toxins; (iv) Determine the face of interaction of both site-3 (Av3) and site-4 (LqhIT2) toxins with insect sodium channels using thermodynamic mutant cycle analysis; and, (v) Examine whether Av3, LqhIT2, pyrethroids, and indoxacarb (belongs to a new generation of insecticides), enhance allosterically the action of one another on the fruit fly and cockroach paraNa-channels and on their kdr and super-kdr mutants. This research establishes the grounds for rational design of novel anti-insect peptidomimetics with minimal impact on human health, and offers a new approach in insect pest control, whereby a combination of allosterically interacting compounds increases insecticidal action and reduces risks of resistance buildup.
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Avni, Adi, and Gitta L. Coaker. Proteomic investigation of a tomato receptor like protein recognizing fungal pathogens. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7600030.bard.
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Maximizing food production with minimal negative effects on the environment remains a long-term challenge for sustainable food production. Microbial pathogens cause devastating diseases, minimizing crop losses by controlling plant diseases can contribute significantly to this goal. All plants possess an innate immune system that is activated after recognition of microbial-derived molecules. The fungal protein Eix induces defense responses in tomato and tobacco. Plants recognize Eix through a leucine-rich-repeat receptor- like-protein (LRR-RLP) termed LeEix. Despite the knowledge obtained from studies on tomato, relatively little is known about signaling initiated by RLP-type immune receptors. The focus of this grant proposal is to generate a foundational understanding of how the tomato xylanase receptor LeEix2 signals to confer defense responses. LeEix2 recognition results in pattern triggered immunity (PTI). The grant has two main aims: (1) Isolate the LeEix2 protein complex in an active and resting state; (2) Examine the biological function of the identified proteins in relation to LeEix2 signaling upon perception of the xylanase elicitor Eix. We used two separate approaches to isolate receptor interacting proteins. Transgenic tomato plants expressing LeEix2 fused to the GFP tag were used to identify complex components at a resting and activated state. LeEix2 complexes were purified by mass spectrometry and associated proteins identified by mass spectrometry. We identified novel proteins that interact with LeEix receptor by proteomics analysis. We identified two dynamin related proteins (DRPs), a coiled coil – nucleotide binding site leucine rich repeat (SlNRC4a) protein. In the second approach we used the split ubiquitin yeast two hybrid (Y2H) screen system to identified receptor-like protein kinase At5g24010-like (SlRLK-like) (Solyc01g094920.2.1) as an interactor of LeEIX2. We examined the role of SlNRC4a in plant immunity. Co-immunoprecipitation demonstrates that SlNRC4a is able to associate with different PRRs. Physiological assays with specific elicitors revealed that SlNRC4a generally alters PRR-mediated responses. SlNRC4a overexpression enhances defense responses while silencing SlNRC4 reduces plant immunity. We propose that SlNRC4a acts as a non-canonical positive regulator of immunity mediated by diverse PRRs. Thus, SlNRC4a could link both intracellular and extracellular immune perception. SlDRP2A localizes at the plasma membrane. Overexpression of SlDRP2A increases the sub-population of LeEIX2 inVHAa1 endosomes, and enhances LeEIX2- and FLS2-mediated defense. The effect of SlDRP2A on induction of plant immunity highlights the importance of endomembrane components and endocytosis in signal propagation during plant immune . The interaction of LeEIX2 with SlRLK-like was verified using co- immunoprecipitation and a bimolecular fluorescence complementation assay. The defence responses induced by EIX were markedly reduced when SlRLK-like was over-expressed, and mutation of slrlk-likeusing CRISPR/Cas9 increased EIX- induced ethylene production and SlACSgene expression in tomato. Co-expression of SlRLK-like with different RLPs and RLKs led to their degradation, apparently through an endoplasmic reticulum-associated degradation process. We provided new knowledge and expertise relevant to expression of specific be exploited to enhance immunity in crops enabling the development of novel environmentally friendly disease control strategies.
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Tabbert, Cory, John Vest, and Marin Kress. Waterway engineering applications of Automatic Identification System data along the Mississippi River and at lock structures. Engineer Research and Development Center (U.S.), 2024. http://dx.doi.org/10.21079/11681/48264.
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The USACE, St. Louis District, is responsible for maintaining navigation channels along with multiple lock and dam structures on the Mississippi River, a vital inland waterway that carries millions of tons of commodities every year. Understanding commercial vessel traffic patterns is fundamental to informing decisions about construction projects and to efforts to improve communication to mariners. Automatic Identification System (AIS) data provides time-stamped and geo-referenced vessel position reports for most commercial vessels operating in the District’s area of interest. This paper describes how AIS data has been successfully used by St. Louis District waterway managers to (1) prevent conflicts with the navigation industry by revealing active fleeting areas that were under consideration for the construction of river training structures; and (2) identify changes in vessel approaches to a lock structure under different river flow conditions, providing operational information that could be used in future navigation alerts to mariners. This paper concludes with a list of suggested best practices for waterways managers who want to start, or expand, their use of AIS data.
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Sanders, Suzanne, and Jessica Kirschbaum. Forest health monitoring at Mississippi National River and Recreation Area: 2022 field season. National Park Service, 2023. http://dx.doi.org/10.36967/2301407.
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The Mississippi National River and Recreation area (MISS), situated along a 116 km stretch of the Mississippi River through the Minneapolis and St. Paul urban corridor, encompasses ~21,800 ha of public and private land. In 2022, the Great Lakes Inventory and Monitoring Network (GLKN) resampled permanent forest monitoring sites in the park, marking the second assessment of these sites, which were established and initially sampled in 2011. The goal of this long-term monitoring project is to provides managers with routine updates on which to base management decisions; these data can also be used to tease apart impacts and elucidate causal agents when novel problems or situations arise. We initiated a comprehensive forest monitoring program at MISS in 2011, establishing 33 sites at that time. High water levels during our sampling window that year precluded sampling on many of our planned sites while on others, water levels had only recently subsided. Here, the full complement of herbs had not yet emerged. In 2022, we resampled existing sites and established additional locations, bringing the total to 50. Sampled and derived metrics included trees (density and basal area of live trees, seedlings, and snags (i.e., standing dead trees)), understory (herb and shrub frequency), browse (bite marks on woody species and presence and height of herbaceous species), and taxa richness. We classified sites into four broad forest types using the newer (2022) dataset, resulting in two upland types (upland rich, upland disturbed) and two floodplain types (box elder-dominated and silver maple-dominated). Because of sampling difficulties in 2011, we are only comparing tree, sapling, and snag data between years. At upland rich sites, overall tree (? 2.5 cm diameter at breast height [DBH]) density declined 22%, while that for just the small sapling component (? 2.5 cm, < 5 cm DBH) fell 41%. Species experiencing notable losses include basswood (Tilia americana L.), elm (Ulmus L.), bitternut hickory (Carya cordiformis (Wangenh.) K. Koch), and red oak (Quercus rubra L.). All three resampled sites are located in Spring Lake Park Reserve and subjected to high white-tailed deer (Odocoileus virginianus Zimm.) browse pressure. We sampled seven sites in upland disturbed forests, where overall tree density fell 17% from 778 ? 215 trees/ha to 648 ? 72 trees/ha, largely due to declines in elm, ash (Fraxinus sp. L.), and hackberry (Celtis occidentalis L.). While changes in black cherry (Prunus serotina Ehrh.) mirrored this pattern in diameter classes above 5 cm, density of saplings increased 12-fold, largely due to a swamping effect from one site, possibly in response to buckthorn (Rhamnus cathartica L.) removal. In the nine box elder-dominated sites, overall tree density declined from 635 ? 47 in 2011 to 500 ? 58 trees/ha in 2022, mainly reflecting changes in box elder (Acer negundo L.), elm, and silver maple (Acer saccharinum L.). In these sites, density of large (? 30 cm DBH) snags increased from 2.5 ? 1.6 to 11.1 ? 4.4 snags/ha. In silver maple-dominated floodplain forests, tree density in the 12 sites fell from 421 ? 63 to 291 ? 23 trees/ha, with declines observed in all five dominant species. Sapling density was low in these sites, falling from 62.6 ? 36 in 2011 to 23.6 ? 11 saplings/ha in 2022. Our observations likely reflect both deer browse and alteration of the flow regime by river impoundment. At upland sites, deer browse is impeding regeneration of all major upland species: red oak, bitternut hickory, basswood, and elm. While browse is also occurring in floodplain sites, prolonged inundation may play a larger role in regeneration failure here. Saplings of silver maple, box elder, cottonwood, elm, and hackberry all have some degree of susceptibility to inundation, ranging from moderate tolerance to completely intolerant. The Mississippi River experienced flooding in 2014, 2017, and again in 2019 when flood stage was exceeded for a record number of days in St. Paul. Sapling decline at floodplain sites is likely a direct result of this. Forest management within the park should focus both on invasive species control and floodplain reforestation. Several sites with heavy invasive weed species are in areas where leveraging local volunteers for removal projects may be possible. Floodplain reforestation requires a dual approach of research and active management. Research is needed to determine preferred propagule types and planting stock, as well as the most effective ways to control invasives, especially reed canarygrass (Phalaris arundinacea L.). Active floodplain reforestation can alleviate many of the issues we found here, although this is expensive, limited in scope, and carries with it a great deal of uncertainty. Nonetheless, projects undertaken at a small scale can provide lessons to managers, based on which aspects were successful and which were not. Many of the park forests at MISS are nearing an inflection point and are at risk of becoming irreversibly altered if countermeasures are not undertaken in the near future. At this point, steps taken to promote ecosystem integrity are likely to be less costly and more effective than those which may be needed after further ecosystem decline. The river system through the Twin Cities metro area provides numerous services, both ecological and otherwise. As the need to act is becoming a pressing issue, it is incumbent on land managers to recognize this, and address it.