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Relevant bibliographies by topics / T-DNA insertion mutant

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Academic literature on the topic 'T-DNA insertion mutant'

Author: Grafiati

Published: 4 June 2021

Last updated: 12 February 2022

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Journal articles on the topic "T-DNA insertion mutant"

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Michielse, Caroline B., Arthur F. J. Ram, Paul J. J. Hooykaas, and Cees A. M. J. J. van den Hondel. "Agrobacterium-Mediated Transformation of Aspergillus awamori in the Absence of Full-Length VirD2, VirC2, or VirE2 Leads to Insertion of Aberrant T-DNA Structures." Journal of Bacteriology 186, no. 7 (2004): 2038–45. http://dx.doi.org/10.1128/jb.186.7.2038-2045.2004.

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ABSTRACT Reductions to 2, 5, and 42% of the wild-type transformation efficiency were found when Agrobacterium mutants carrying transposon insertions in virD2, virC2, and virE2, respectively, were used to transform Aspergillus awamori. The structures of the T-DNAs integrated into the host genome by these mutants were analyzed by Southern and sequence analyses. The T-DNAs of transformants obtained with the virE2 mutant had left-border truncations, whereas those obtained with the virD2 mutant had truncated right ends. From this analysis, it was concluded that the virulence proteins VirD2 and VirE

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Tanguay, Philippe, Kristin Tangen, and Colette Breuil. "Identifying Pigmentation-Related Genes in Ophiostoma piceae Using Agrobacterium-Mediated Integration." Phytopathology® 97, no. 9 (2007): 1040–48. http://dx.doi.org/10.1094/phyto-97-9-1040.

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Wood sapstain, a cosmetic defect that results in significant economical loss to forest-products industries, is caused by mycelial melanization of the wood-colonizing ophiostomatoid fungi. To improve our understanding of how melanin biosynthesis is regulated in the cosmopolitan sapstaining fungus, Ophiostoma piceae, we used insertional mutagenesis. Insertional mutants were generated by restriction enzyme-mediated integration (REMI) and Agrobacterium-mediated integration (AMI). We screened 1,053 REMI and 1,083 AMI transformants and found 30 mutants with impaired growth or pigmentation. We charac

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McNevin, John P., Wendy Woodward, Abdelali Hannoufa, Kenneth A. Feldmann, and Bertrand Lemieux. "Isolation and characterization of eceriferum (cer) mutants induced by T-DNA insertions in Arabidopsis thaliana." Genome 36, no. 3 (1993): 610–18. http://dx.doi.org/10.1139/g93-082.

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Thirteen Arabidopsis thaliana mutants with deviating epicuticular wax layers (i.e., cer mutants) were isolated by screening 13 000 transformed lines produced by the seed transformation method. After crossing the 13 mutants to some of the previously known cer mutant lines, 12 of our mutants mapped to 6 of the 21 known complementation groups (cer1 through cer4 as well as cer6 and cer10), while the other mutant corresponded to a previously unknown locus, cer21. Mutant phenotypes of 6 of the 13 mutant lines were caused by T-DNA insertions within cer genes. We also analyzed the chemical composition

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Koerniati, Sri. "UNTAGGED MUTATION IN RICE GAL4/VP16 TRANSCRIPTIONAL ACTIVATOR FACILITATED-ENHANCER TRAP LINES." Indonesian Journal of Agricultural Science 14, no. 1 (2013): 27. http://dx.doi.org/10.21082/ijas.v14n1.2013.27-35.

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An enhancer trap system is an insertional mutagenesis based upon gene expression, instead of gene knock-out, so its insertion in genome is expected not linked to any dramatic changes in plant phenotypes. Gene knock-out, leading to lossof- function (LoF) mutation, is a dominant approach for rice functional genomic studies. The objective of this study was to find out whether Transcriptional Activator-Facilitated Enhancer Trap (TAFET) T-DNA insertion inducing mutant phenotypes in rice TAFET population. Materials used in this experiment were T1 generation of 270 rice TAFET lines. Eight plants of e

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Koerniati, Sri. "UNTAGGED MUTATION IN RICE GAL4/VP16 TRANSCRIPTIONAL ACTIVATOR FACILITATED-ENHANCER TRAP LINES." Indonesian Journal of Agricultural Science 14, no. 1 (2013): 27. http://dx.doi.org/10.21082/ijas.v14n1.2013.p27-35.

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An enhancer trap system is an insertional mutagenesis based upon gene expression, instead of gene knock-out, so its insertion in genome is expected not linked to any dramatic changes in plant phenotypes. Gene knock-out, leading to lossof- function (LoF) mutation, is a dominant approach for rice functional genomic studies. The objective of this study was to find out whether Transcriptional Activator-Facilitated Enhancer Trap (TAFET) T-DNA insertion inducing mutant phenotypes in rice TAFET population. Materials used in this experiment were T1 generation of 270 rice TAFET lines. Eight plants of e

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Richardson, Kim, Sarah Fowler, Carly Pullen, Caryl Skelton, Bret Morris, and Jo Putterill. "T-DNA tagging of a flowering-time gene and improved gene transfer by in planta transformation of Arabidopsis." Functional Plant Biology 25, no. 1 (1998): 125. http://dx.doi.org/10.1071/pp97109.

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Gene tagging with insertional mutagens greatly facilitates the isolation of novel genes. A new collection of Arabidopsis T-DNA tag insertion lines (n=2165) was generated by in planta transformation. Whole plants were vacuum-infiltrated in a suspension of Agrobacterium carrying the pGKB5 tagging vector. The efficiency of transformation increased with addition of the surfactant Silwet L-77 (0.005% v/v) to the Agrobacterium suspension. Visual screens of the T-DNA lines identified two mutants with floral defects. Allelism tests suggested that a mutation in the GIGANTEA gene was responsible for the

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Martinelli, Laurie. "Australian Journal of Plant Physiology: Editorial report." Functional Plant Biology 25, no. 1 (1998): I. http://dx.doi.org/10.1071/ppv25n1_ed.

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Gene tagging with insertional mutagens greatly facilitates the isolation of novel genes. A new collection of Arabidopsis T-DNA tag insertion lines (n=2165) was generated by in planta transformation. Whole plants were vacuum-infiltrated in a suspension of Agrobacterium carrying the pGKB5 tagging vector. The efficiency of transformation increased with addition of the surfactant Silwet L-77 (0.005% v/v) to the Agrobacterium suspension. Visual screens of the T-DNA lines identified two mutants with floral defects. Allelism tests suggested that a mutation in the GIGANTEA gene was responsible for the

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Feldmann, K. A., M. D. Marks, M. L. Christianson, and R. S. Quatrano. "A Dwarf Mutant of Arabidopsis Generated by T-DNA Insertion Mutagenesis." Science 243, no. 4896 (1989): 1351–54. http://dx.doi.org/10.1126/science.243.4896.1351.

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Xu, Liangsheng, and Weidong Chen. "Random T-DNA Mutagenesis Identifies a Cu/Zn Superoxide Dismutase Gene as a Virulence Factor of Sclerotinia sclerotiorum." Molecular Plant-Microbe Interactions® 26, no. 4 (2013): 431–41. http://dx.doi.org/10.1094/mpmi-07-12-0177-r.

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Agrobacterium-mediated transformation (AMT) was used to identify potential virulence factors in Sclerotinia sclerotiorum. Screening AMT transformants identified two mutants showing significantly reduced virulence. The mutants showed growth rate, sclerotial formation, and oxalate production similar to that of the wild type. The mutation was due to a single T-DNA insertion at 212 bp downstream of the Cu/Zn superoxide dismutase (SOD) gene (SsSOD1, SS1G_00699). Expression levels of SsSOD1 were significantly increased under oxidative stresses or during plant infection in the wild-type strain but co

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Elliott, Candace E., and Barbara J. Howlett. "Overexpression of a 3-Ketoacyl-CoA Thiolase in Leptosphaeria maculans Causes Reduced Pathogenicity on Brassica napus." Molecular Plant-Microbe Interactions® 19, no. 6 (2006): 588–96. http://dx.doi.org/10.1094/mpmi-19-0588.

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Agrobacterium tumefaciens-mediated random mutagenesis was used to generate insertional mutants of the fungus Leptosphaeria maculans. Of 91 transformants screened, only one (A3) produced lesions of reduced size on cotyledons of canola (Brassica napus). Genes flanking the T-DNA insertion had the best matches to an alcohol dehydrogenase class 4 (ADH4)-like gene (Adh4L) and a 3-ketoacyl-CoA thiolase gene (Thiol) and were expressed in mutant A3 in vitro and in planta at significantly higher levels than in the wild type. This is the first report of a T-DNA insertion in fungi causing increased gene e

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Dissertations / Theses on the topic "T-DNA insertion mutant"

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Njoroge, Norman Chege. "Physiological and genetic characterization of T-DNA insertion mutants of Arabidopsis thaliana that are tolerant to salt." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0001/NQ42963.pdf.

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Ruiz-Rojas, Juan Jairo. "Characterization of T-DNA integration sites within a population of insertional mutants of the diploid strawberry Fragaria vesca L." Diss., Virginia Tech, 2010. http://hdl.handle.net/10919/77264.

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Cultivated strawberry (Fragaria × ananassa) is an octoploid (2n=8x=56) species that belongs to the Rosaceae family and the high ploidy level makes genetic and molecular studies difficult. However, its commercial success because of its unique flavor and nutritious qualities has increased interest in the development of genomic resources. Fragaria vesca L. is a diploid (2n=2x=14) species with a small genome size (206 Mbp), short reproductive cycle, and facile vegetative and seed propagation that make it an attractive model for genomic studies. The availability of an efficient transformation metho

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Briju, Betsy J. "Progress of Work towards Cloning Gravity Persistence Signal (gps) Mutants by PCR-Based Methods and Positional Mapping." Ohio University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1320862556.

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Weißleder, Andrea [Verfasser], Veit Akademischer Betreuer] Schubert, Klaus [Akademischer Betreuer] [Humbeck, and Thomas [Akademischer Betreuer] Schmidt. "Selection and characterization of Arabidopsis thaliana cohesin and condensin T-DNA insertion mutants / Andrea Weißleder. Betreuer: Veit Schubert ; Klaus Humbeck ; Thomas Schmidt." Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2010. http://d-nb.info/1024976017/34.

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Hsu, Yang, and 許揚. "Functional characterization of a pathogen susceptible T-DNA insertion mutant- M23454." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/b567m7.

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碩士<br>國立中興大學<br>分子生物學研究所<br>107<br>A rice T-DNA insertion mutant M23454 revealed susceptible to pathogens and prone to form leaf lesions during the transition from vegetative growth stage to reproductive stage. This mutant has its T-DNA inserted in the rice chromosome #4 within the BAC clone OSJNBa0039C07 region. All predicted genes flanked to the insertion site within this BAC clone region were analyzed. The flanking gene named 454-10 was the only one significantly activated in the mutant; however, transgenic line over-expressing a full-length cDNA of 454-10 did not recapitulate the phenotype

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Wong, Lai-In, and 黃麗燕. "Rice functional genomics study using T-DNA insertion mutants: characterization of plant height mutant M82268." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/79604492722152410529.

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碩士<br>國立中興大學<br>分子生物學研究所<br>97<br>Seven rice T-DNA insertion mutant lines with altered plant height were identified in this study. The plant height of M82268 was about 20~30% higher than that of the wild type TNG67. M82268 has its T-DNA inserted at 80,783 bp of BAC clone OJ1657_H11 on the chromosome number 5. The progeny assay from T2 to T4 generations demonstrated that the taller phenotype of M82268 was co-segregated with the T-DNA insertion and there is only one T-DNA insertion event in the genome. Two flanking genes, GID gibberellin receptor and putative LEC14B, located either 14.0 kb upstr

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Lin, En-Te, and 林恩德. "Rice functional genomics study using T‐DNA insertion mutants - characterization of a special mutant 48000A." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/64046887259527927242.

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碩士<br>國立中興大學<br>分子生物學研究所<br>100<br>The purpose of this study is try to elucidate the possible cause of the delay germination, shorter plant height and lighter grain weight in a T-DNA insertion rice mutant 48000A. This mutant has its T-DNA inserted at the 147,606th base of BAC OJ1344_B01 on chromosome number 9 and seven annotated genes OA-25, OA-27, OA-29, OA-30, OA-31, OA-36, OA-40 were identified within a 30 kb region flanking to the T-DNA insertion site. Four of them OA-25, OA-29, OA-30, OA-31 were activated in this mutant and they were annotated as the putative Flavonoid 3''- hyd

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Shen, Meng-Ni, and 沈盟倪. "Rice functional genomics study with T-DNA insertion mutants-Functional analysis of a disease susceptible mutant M0023454." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/18632580014779072000.

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碩士<br>國立中興大學<br>分子生物學研究所<br>96<br>Rice (Oryza sativa L. cv. Tainung 67) mutant line, M0023454, is susceptible to rice blast that may resulted by the activation of a T-DNA insertion. Eight CaMV35S enhancers were constructed in T-DNA that can activate the expression of flanking genes next to its insertion. AP2/ERF (APETALA2/ethylene response factor) domain-containing gene 454-10 (Os04g32620), a flanking gene next to the T-DNA insertion in M0023454, is classified into B4 subcluster of ERF subfamily in AP2/ERF superfamily. Phylogenic analysis suggested that 454-10 is an AP2/ERF-like transcription

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Ming-LungCheng and 鄭明龍. "Mining for novel genes regulating rice development from the T-DNA insertion mutant population." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/wjs976.

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博士<br>國立成功大學<br>生命科學系<br>105<br>Rice is the most important staple food in the world and supports over 50% of the human population. Sequencing of the whole genome of rice was finished in 2005, which predicted at least 30,000 genes. The sequence data provide good bioinformatics information for rice gene functional studies. The Taiwan Rice Insertional Mutants (TRIM) population contains at least 100,000 T-DNA insertion mutant lines, making this a valuable resource and efficient tool for rice gene discoveries. By adopting a forward genetics approach, we screened the TRIM population and found an int

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KUNG, YUEH-JEN, and 龔月珍. "Rice functional genomics study with T-DNA insertion mutant— Characterization and gene expression analysis of a heading development mutant M0034845." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/49051891973663765040.

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碩士<br>國立中興大學<br>分子生物學研究所<br>93<br>In this study, the T-DNA insertion mutant pool created by Dr Yu’s group of Academia Sinica was used for screening distinct phenotype in grain development such as sterile, low fertility or low grain yield. Twenty-three mutants were selected initially for flanking sequence identification through plasmid rescue and/or thermal asymmetric interlaced PCR analyses. Among them, eleven mutants of their flanking sequences could be resolved and 5 of them revealed only single T-DNA insertion with the same Tos17 hybridization pattern as that of TNG67 after Southern blott

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Book chapters on the topic "T-DNA insertion mutant"

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O’Malley, Ronan C., Cesar C. Barragan, and Joseph R. Ecker. "A User’s Guide to the Arabidopsis T-DNA Insertion Mutant Collections." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2444-8_16.

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Winkler, Rodney G., and Kenneth A. Feldmann. "PCR-Based Identification of T-DNA Insertion Mutants." In Arabidopsis Protocols. Humana Press, 1998. http://dx.doi.org/10.1385/0-89603-391-0:129.

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Coury, D. A., and K. A. Feldmann. "T-DNA Insertion Mutagenesis and the Untagged Mutants." In Somaclonal Variation and Induced Mutations in Crop Improvement. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-015-9125-6_26.

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Qu, Li-Jia, and Genji Qin. "Generation and Characterization of Arabidopsis T-DNA Insertion Mutants." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-580-4_13.

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Şahİn, S., S. D. GÜrle, and Ş. Ari. "Genetic Analyses on the T-Dna Insertion Mutants of Tobacco (Nicotiana Tabacum)." In Progress in Botanical Research. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5274-7_127.

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Jung, Ki-Hong, and Gynheung An. "Functional Characterization of Rice Genes Using a Gene-Indexed T-DNA Insertional Mutant Population." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-194-3_5.

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Kim, Sung-Ryul, Jong-Seong Jeon, and Gynheung An. "Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice." In Methods in Molecular Biology. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-682-5_11.

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Conference papers on the topic "T-DNA insertion mutant"

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Xu, Peidong, Xiaolan Zheng, Wen Tang, et al. "The analysis of T-DNA insertional Colletotrichum gloeosporioides in Stylo pathogenicity-weakened mutant strain 1681." In 3rd International Conference on Material, Mechanical and Manufacturing Engineering (IC3ME 2015). Atlantis Press, 2015. http://dx.doi.org/10.2991/ic3me-15.2015.43.

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