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Dissertations / Theses on the topic 'T-DNA insertion mutant'

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Published: 4 June 2021

Last updated: 12 February 2022

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1

Njoroge, Norman Chege. "Physiological and genetic characterization of T-DNA insertion mutants of Arabidopsis thaliana that are tolerant to salt." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0001/NQ42963.pdf.

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2

Ruiz-Rojas, Juan Jairo. "Characterization of T-DNA integration sites within a population of insertional mutants of the diploid strawberry Fragaria vesca L." Diss., Virginia Tech, 2010. http://hdl.handle.net/10919/77264.

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Cultivated strawberry (Fragaria × ananassa) is an octoploid (2n=8x=56) species that belongs to the Rosaceae family and the high ploidy level makes genetic and molecular studies difficult. However, its commercial success because of its unique flavor and nutritious qualities has increased interest in the development of genomic resources. Fragaria vesca L. is a diploid (2n=2x=14) species with a small genome size (206 Mbp), short reproductive cycle, and facile vegetative and seed propagation that make it an attractive model for genomic studies. The availability of an efficient transformation metho

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3

Briju, Betsy J. "Progress of Work towards Cloning Gravity Persistence Signal (gps) Mutants by PCR-Based Methods and Positional Mapping." Ohio University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1320862556.

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4

Weißleder, Andrea [Verfasser], Veit Akademischer Betreuer] Schubert, Klaus [Akademischer Betreuer] [Humbeck, and Thomas [Akademischer Betreuer] Schmidt. "Selection and characterization of Arabidopsis thaliana cohesin and condensin T-DNA insertion mutants / Andrea Weißleder. Betreuer: Veit Schubert ; Klaus Humbeck ; Thomas Schmidt." Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2010. http://d-nb.info/1024976017/34.

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5

Hsu, Yang, and 許揚. "Functional characterization of a pathogen susceptible T-DNA insertion mutant- M23454." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/b567m7.

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碩士<br>國立中興大學<br>分子生物學研究所<br>107<br>A rice T-DNA insertion mutant M23454 revealed susceptible to pathogens and prone to form leaf lesions during the transition from vegetative growth stage to reproductive stage. This mutant has its T-DNA inserted in the rice chromosome #4 within the BAC clone OSJNBa0039C07 region. All predicted genes flanked to the insertion site within this BAC clone region were analyzed. The flanking gene named 454-10 was the only one significantly activated in the mutant; however, transgenic line over-expressing a full-length cDNA of 454-10 did not recapitulate the phenotype

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6

Wong, Lai-In, and 黃麗燕. "Rice functional genomics study using T-DNA insertion mutants: characterization of plant height mutant M82268." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/79604492722152410529.

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碩士<br>國立中興大學<br>分子生物學研究所<br>97<br>Seven rice T-DNA insertion mutant lines with altered plant height were identified in this study. The plant height of M82268 was about 20~30% higher than that of the wild type TNG67. M82268 has its T-DNA inserted at 80,783 bp of BAC clone OJ1657_H11 on the chromosome number 5. The progeny assay from T2 to T4 generations demonstrated that the taller phenotype of M82268 was co-segregated with the T-DNA insertion and there is only one T-DNA insertion event in the genome. Two flanking genes, GID gibberellin receptor and putative LEC14B, located either 14.0 kb upstr

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Lin, En-Te, and 林恩德. "Rice functional genomics study using T‐DNA insertion mutants - characterization of a special mutant 48000A." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/64046887259527927242.

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碩士<br>國立中興大學<br>分子生物學研究所<br>100<br>The purpose of this study is try to elucidate the possible cause of the delay germination, shorter plant height and lighter grain weight in a T-DNA insertion rice mutant 48000A. This mutant has its T-DNA inserted at the 147,606th base of BAC OJ1344_B01 on chromosome number 9 and seven annotated genes OA-25, OA-27, OA-29, OA-30, OA-31, OA-36, OA-40 were identified within a 30 kb region flanking to the T-DNA insertion site. Four of them OA-25, OA-29, OA-30, OA-31 were activated in this mutant and they were annotated as the putative Flavonoid 3''- hyd

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Shen, Meng-Ni, and 沈盟倪. "Rice functional genomics study with T-DNA insertion mutants-Functional analysis of a disease susceptible mutant M0023454." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/18632580014779072000.

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碩士<br>國立中興大學<br>分子生物學研究所<br>96<br>Rice (Oryza sativa L. cv. Tainung 67) mutant line, M0023454, is susceptible to rice blast that may resulted by the activation of a T-DNA insertion. Eight CaMV35S enhancers were constructed in T-DNA that can activate the expression of flanking genes next to its insertion. AP2/ERF (APETALA2/ethylene response factor) domain-containing gene 454-10 (Os04g32620), a flanking gene next to the T-DNA insertion in M0023454, is classified into B4 subcluster of ERF subfamily in AP2/ERF superfamily. Phylogenic analysis suggested that 454-10 is an AP2/ERF-like transcription

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9

Ming-LungCheng and 鄭明龍. "Mining for novel genes regulating rice development from the T-DNA insertion mutant population." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/wjs976.

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博士<br>國立成功大學<br>生命科學系<br>105<br>Rice is the most important staple food in the world and supports over 50% of the human population. Sequencing of the whole genome of rice was finished in 2005, which predicted at least 30,000 genes. The sequence data provide good bioinformatics information for rice gene functional studies. The Taiwan Rice Insertional Mutants (TRIM) population contains at least 100,000 T-DNA insertion mutant lines, making this a valuable resource and efficient tool for rice gene discoveries. By adopting a forward genetics approach, we screened the TRIM population and found an int

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KUNG, YUEH-JEN, and 龔月珍. "Rice functional genomics study with T-DNA insertion mutant— Characterization and gene expression analysis of a heading development mutant M0034845." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/49051891973663765040.

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碩士<br>國立中興大學<br>分子生物學研究所<br>93<br>In this study, the T-DNA insertion mutant pool created by Dr Yu’s group of Academia Sinica was used for screening distinct phenotype in grain development such as sterile, low fertility or low grain yield. Twenty-three mutants were selected initially for flanking sequence identification through plasmid rescue and/or thermal asymmetric interlaced PCR analyses. Among them, eleven mutants of their flanking sequences could be resolved and 5 of them revealed only single T-DNA insertion with the same Tos17 hybridization pattern as that of TNG67 after Southern blott

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11

Li, Yan-Suan, and 李彥璇. "Rice functional genomics study with T-DNA insertion mutants—Characterization and gene expression analysis of a disease susceptible mutant M0023454 and a seed development mutant M0039314." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/13266446060857175347.

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碩士<br>國立中興大學<br>分子生物學研究所<br>94<br>In this study, 12 T-DNA insertion mutant lines were selected by their distinct phenotypes in grain development such as sterile, low fertility, abnormal panicles and grains. Only six of these mutants could get flanking sequence by TAIL PCR and plasmid rescue. Among them, only mutants M0023454 and M0039314 showed single insertion event without changes of Tos17 number in their genomes. They were selected for further study. In mutant M0023454, the T-DNA was inserted in the BAC clone of OSJNBa0039C07 in the rice chromosome number 4. This mutant showed more suscepti

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12

Wu, Tsai-Hsin, and 吳采馨. "Rice functional genomics study with T-DNA insertion mutants--Characterizetion and gene expression analysis of a growth and development mutant M0019081." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/51500757130429396368.

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碩士<br>國立中興大學<br>分子生物學研究所<br>93<br>In this study, the T-DNA insertion mutant pool created by Dr Yu’s group of Academia Sinica was used for screening distinct phenotype in grain development such as sterile, low fertility or low grain yield. Twenty-two mutants were selected initially for flanking sequence identification through plasmid rescue and/or thermal asymmetric interlaced PCR analyses. Ten mutants of their flanking sequences were resolved. Among them, a dwarf and low fertility mutant M0019081 showing a single copy of T-DNA insertion in the chromosome 7 within the BAC clone OJ1316_A04 wa

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13

chung, chêng yao, and 鄭堯中. "The study of flanking sequences generate methods and the insertion sites of rice T-DNA mutant lines." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/89861213854565266510.

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碩士<br>亞洲大學<br>生活應用科學學系碩士班<br>95<br>The researches engaged in the functional genomic of rice at present mainly used T-DNA as tag to understand the profits of rice gene and there function. In addition, for the purpose of reverse genetic, the research from gene sequence to the phenotype by T-DNA insertion the inseration sites flanking sequence is very importment.Which can let us understands the connection between phenotypes and their gene sequences of those mutane lines. The database of T-DNA insertion sites (Flanking sequence tag, FST) which can accelerate the research of the rice functional gen

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14

Li, Tzu-Yin, and 李咨胤. "Characterization of the T-DNA insertion mutant M52048 and functional study of three activated genes OsMADS34、OsMADS14 and OsCP7." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/gp7a2s.

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碩士<br>國立中興大學<br>分子生物學研究所<br>99<br>A rice T-DNA insertion mutant M0052048 showing extreme early flowering, bending tillers and impairment in panicle exertion was isolated from the Taiwan Rice Insertion Mutant (TRIM) library. This mutant contained a copy of the T-DNA tag inserted in the chromosome number 3 at the 64,867 bp position of OSJBa0032E21 BAC clone where the expression of 048-3 (OsMADS34), 048-4 (OsMADS14) and 048-7 (putative cysteine proteinase, OsCP7) genes were activated by the 35S enhancer located in the T-DNA. The OsMADS14 and OsMADS34 are MADS box-containing transcription factors

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15

Chang, Yu-Pei, and 張渝珮. "Characterization of the T-DNA insertion mutant M69217 and gene functional study of two activated genes, OsCM and OsCCR." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/15865474158396037817.

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碩士<br>國立中興大學<br>分子生物學研究所<br>101<br>The rice T-DNA insertion mutant M69217 showed semi-dwarf, high tiller number, short panical length and low fertility rate. It’s T-DNA inserted at the 36,560 bp of BAC clone OJ1297_C09 of chromosome number 2 and the expression of Gene-07（putative chorismate mutase, CM）、Gene-09（putative cinnamoyl-CoA reductase, CCR）were activated. CM is the first and critical enzyme of the aromatic amino acids tyrosine and phenylalanine biosynthesis, CCR is also a key enzyme in the formation of lignin. To study the contributions of CM and CCR to the phenotype of M69217, putativ

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Tsai, Chia-Fen, and 蔡佳芬. "Generation of Nicotiana benthamiana Mutant Lines by T-DNA Insertion Mutagenesis for Study of Host factors Involved in Bamboo mosaic virus Replication." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/83324862327178158755.

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碩士<br>國立中興大學<br>生物科技學研究所<br>91<br>The infection of plants by viruses usually occurs through complex interactions between virus-encoded and host-encoded factors. Some evidences reveal that host factors are involved in the process of virus RNA replication, such as those interacted with RdRP and other cellular translational factors. But the information about the host factors that directly participate in the virus RNA replication is limited. The aim of this study is to generate a large number of Nicotiana benthamiana mutants by T-DNA insertion mutagenesis, for screening of mutant lines in which Ba

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17

Pai, Yu-Min, and 白育旻. "Searching for transcription factors involved in regulating expression of GA2ox genes and characterization of a high tiller number T-DNA insertion mutant M69217." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/16729582236393637615.

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碩士<br>國立中興大學<br>分子生物學研究所<br>103<br>GA (gibberellic acids) is one of the plant hormones involved in plants development, excessive GA cause plant lodging and lack of GA cause plant dwarf. GA2-oxidases are enzymes catalyze bioactive GA or GA precursors to inactive GAs and to regulate GA homeostasis in plants. There are nine GA2-oxidase genes in rice (OsGA2ox1–9) and how these gene expressions are regulated temporally and spatially in rice plant have not yet been characterized. This study intended to search for transcription factors involved in regulating the expression of OsGA2ox genes. After bio

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18

Houde, Josée. "Caractérisation d'une famille de récepteurs kinases impliqués dans le développement gamétophytique chez Arabidopsis thaliana." Thèse, 2010. http://hdl.handle.net/1866/3756.

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Au cours du développement des végétaux, de l’établissement de l’identité cellulaire des premiers organes au guidage du tube pollinique, la communication cellule à cellule est d’une importance capitale. En réponse, les voies de signalisation moléculaires sont élaborées pour la perception d’un signal extérieur et la transduction en une réponse génique via une cascade intracellulaire. Les récepteurs kinases font partie des protéines perceptrices des stimuli et constituent chez les plantes une catégorie de protéines avec une occurrence considérable, mais dont très peu d’informations détaillées son

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19

Hsiao, Shi-Xuan, and 蕭詩軒. "Physiological mechanism of glufosinate resistance for T-DNA insertion mutants of rice." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/62836370530017740228.

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碩士<br>國立中興大學<br>農藝學系所<br>94<br>The physiological basis of glufosinate resistance for the rice mutants derived from T-DNA insertion-induced mutant pool, resistant (R) line 084-5-1-1(R) , as compared with susceptible (S) mutant line 283-0-4 (S) and a reference variety FSK, was studied. Based on the dose-response analysis and survival rate of 4-leaf rice seedlings 7 days after treatment with 7.54 mM glufosinate, mutant line 084-5-1-1(R) expressed a higher resistante to glufosinate. A 14C-glufosinate feeding experiment showed no difference among 3 rice lines in both uptake and translocation of glu

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Yin-Ju, Ho. "Funtional study of a T-DNA insertional mutant, TPR8, with serrated leaves in Arabidopsis thaliana." 2006. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2607200601374900.

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Ho, Yin-Ju, and 何盈儒. "Funtional study of a T-DNA insertional mutant, TPR8, with serrated leaves in Arabidopsis thaliana." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/39301460439307862635.

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碩士<br>國立臺灣大學<br>植物科學研究所<br>94<br>The regulatory mechanism of leaf morphogenesis is complex and influenced by a lot of factors, such as the polar growth, the distribution of phytohormones, and the cell differentiation and so on. We used T-DNA containing 2x35S:Bar:SK to transform Arabidopsis for screening mutants with abnormal leaf phenotypes for further investigating the influences and the functions of genes that are correlated with the mutant phenotype. We focused on a gain-of-function mutant, TPR8, with the At4g01245 overexpressed, with leaves from the fourth leaf to the later sprouting leave

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22

Yu, Cheng-Chun, and 余承駿. "Rice functional genomics analysis of T-DNA insertion mutants, disease susceptible M0023454 and high tillering M0111350." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/33330334759833638895.

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碩士<br>國立中興大學<br>分子生物學研究所<br>101<br>M0023454 and M0111350 are T-DNA mutants constructed by TRIM database. Genomic functionalizations of these phenotypically specific mutants were expected to shed lights on disease resistance and tillering regulation in rice. M0023454 was previously demonstrated pathogen susceptible and lesion formation prone in the transition from vegetative growth stage to reproductive stage. The flanking gene 454-10 was activated in mutant, however, the Ubi::454-10 transgenic line did not phenocopy that of M0023454. To reveal its exact mechanism, all predicted genes in T-DN

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23

You, Jia-Wen, and 游佳雯. "Functional study of a T-DNA insertional mutant with curledleaves and delayed flowering in Arabidopsis thaliana." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/22970184109548726346.

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碩士<br>國立臺灣大學<br>植物科學研究所<br>93<br>Leaves are the most noticeable plant organs and play important roles in photosynthesis, respiration and gas exchange. However, the details of leaf development remain unclear. Therefore, our laboratory used forward genetics to identify the mutants with leaf development defect from T-DNA tagging pools in Arabidopsis. We use a T-DNA tagging vector, pPZP202: BAR: SK, to generate mutants and to screen for morphological mutants. In this study, we have identified a mutant, R1-2, which were selected in the T2 generation, with curled leaves and delayed flowering phenoty

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24

Oh, Tshai-Ta, and 何采達. "Rice Functional Genomics Study with T-DNA Insertion Mutants －Characterization and Gene Expression Analysis of the Slender Seed Mutants M0073694 and M0068597." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/6p5dgn.

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碩士<br>國立陽明大學<br>生命科學系暨基因體科學研究所<br>102<br>Two T-DNA insertion mutants M0068597 showing thin culms, narrow leaves, sparse panicles, abnormal panicles and slender grains were identified from the Taiwan Rice Insertional Mutagenesis (TRIM) library. These two mutants each contains a T-DNA inserted at the positions 66275 and 67533 of a PAC clone: P0710E05 located in chromosome 1. Three genes: Os01g9450(OsIAA2), Os01g09460(OsHXK8) and Os01g09470(OsIQD22) were found to be activated by the 35S enhancer located in T-DNA. OsIAA2, expressed universally in the whole plant, is a putative AUX/IAA protein fami

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Chen, Chi-Wen, and 陳季緯. "Rice functional genomics study with T-DNA insertion mutants--Characterization and gene expression analysis of fertility and development mutants, M0017031 and M0017091." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/75035166360087162933.

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碩士<br>國立中興大學<br>分子生物學研究所<br>94<br>In this study, seven T-DNA insertion mutant lines, M0017031, M0017091, M0019675, M0019845, M0023854, M0023858 and M0030289, were selected by their distinct grain development phenotypes. DNA sequences flanked to T-DNA insertion sites for each mutant were obtained by plasmid rescue and TAIL-PCR. Confirmation of the T-DNA insertion events and their co-segregation with the phenotype were performed through progeny genotyping and phenotype observation. Among them, only mutants M0017031 and M0017091 had well co-segregation between genotype and phenotype. They were t

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You, Jia-Wen. "Functional study of a T-DNA insertional mutant with curled leaves and delayed flowering in Arabidopsis thaliana." 2005. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2207200512552500.

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Chen, Jia-Jyun, and 陳佳君. "Building a Model for Predicting the Target Gene Expression in Rice T-DNA Insertional Mutants by Machine Learning Approaches." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/84199971425107239019.

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碩士<br>國立中興大學<br>基因體暨生物資訊學研究所<br>105<br>Rice is the most important human food crop in the world. In the shadow of food crisis, understanding the gene function of rice is important to promote the yield and the environment tolerance of rice. The T-DNA insertion mutants carried CaMV 35S enhancer which could activate the expression of flanking genes. Previous studies showed that the expression of flanking gene could not be judged directly by the distance between the enhancer and the gene. Thence, it would be time-consuming and laborious works to check the expression of flanking genes. In this study

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Yee, Donna. "The Expanding Diversity of Plant U-box E3 Ubiquitin Ligases in Arabidopsis: Identifying AtPUB18 and AtPUB19 Function during Abiotic Stress Responses." Thesis, 2010. http://hdl.handle.net/1807/26265.

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The ability of plants to sense and respond to environmental and endogenous signals is essential to their growth and development. As part of these diverse cellular functions, ubiquitin-mediated proteolysis has emerged to be an important process involved in how plant signalling pathways can be regulated in response to such cues. Of the three enzymes involved in linking ubiquitin to protein targets, E3 ubiquitin ligases are of interest as they confer substrate specificity during this ubiquitination process. The overall focal point of this research is on plant U-box (PUB) E3 ubiquitin ligases,

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