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Journal articles on the topic 'T-DNA insertion mutant'

Author: Grafiati

Published: 4 June 2021

Last updated: 12 February 2022

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1

Michielse, Caroline B., Arthur F. J. Ram, Paul J. J. Hooykaas, and Cees A. M. J. J. van den Hondel. "Agrobacterium-Mediated Transformation of Aspergillus awamori in the Absence of Full-Length VirD2, VirC2, or VirE2 Leads to Insertion of Aberrant T-DNA Structures." Journal of Bacteriology 186, no. 7 (2004): 2038–45. http://dx.doi.org/10.1128/jb.186.7.2038-2045.2004.

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ABSTRACT Reductions to 2, 5, and 42% of the wild-type transformation efficiency were found when Agrobacterium mutants carrying transposon insertions in virD2, virC2, and virE2, respectively, were used to transform Aspergillus awamori. The structures of the T-DNAs integrated into the host genome by these mutants were analyzed by Southern and sequence analyses. The T-DNAs of transformants obtained with the virE2 mutant had left-border truncations, whereas those obtained with the virD2 mutant had truncated right ends. From this analysis, it was concluded that the virulence proteins VirD2 and VirE

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2

Tanguay, Philippe, Kristin Tangen, and Colette Breuil. "Identifying Pigmentation-Related Genes in Ophiostoma piceae Using Agrobacterium-Mediated Integration." Phytopathology® 97, no. 9 (2007): 1040–48. http://dx.doi.org/10.1094/phyto-97-9-1040.

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Wood sapstain, a cosmetic defect that results in significant economical loss to forest-products industries, is caused by mycelial melanization of the wood-colonizing ophiostomatoid fungi. To improve our understanding of how melanin biosynthesis is regulated in the cosmopolitan sapstaining fungus, Ophiostoma piceae, we used insertional mutagenesis. Insertional mutants were generated by restriction enzyme-mediated integration (REMI) and Agrobacterium-mediated integration (AMI). We screened 1,053 REMI and 1,083 AMI transformants and found 30 mutants with impaired growth or pigmentation. We charac

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3

McNevin, John P., Wendy Woodward, Abdelali Hannoufa, Kenneth A. Feldmann, and Bertrand Lemieux. "Isolation and characterization of eceriferum (cer) mutants induced by T-DNA insertions in Arabidopsis thaliana." Genome 36, no. 3 (1993): 610–18. http://dx.doi.org/10.1139/g93-082.

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Thirteen Arabidopsis thaliana mutants with deviating epicuticular wax layers (i.e., cer mutants) were isolated by screening 13 000 transformed lines produced by the seed transformation method. After crossing the 13 mutants to some of the previously known cer mutant lines, 12 of our mutants mapped to 6 of the 21 known complementation groups (cer1 through cer4 as well as cer6 and cer10), while the other mutant corresponded to a previously unknown locus, cer21. Mutant phenotypes of 6 of the 13 mutant lines were caused by T-DNA insertions within cer genes. We also analyzed the chemical composition

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4

Koerniati, Sri. "UNTAGGED MUTATION IN RICE GAL4/VP16 TRANSCRIPTIONAL ACTIVATOR FACILITATED-ENHANCER TRAP LINES." Indonesian Journal of Agricultural Science 14, no. 1 (2013): 27. http://dx.doi.org/10.21082/ijas.v14n1.2013.27-35.

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An enhancer trap system is an insertional mutagenesis based upon gene expression, instead of gene knock-out, so its insertion in genome is expected not linked to any dramatic changes in plant phenotypes. Gene knock-out, leading to lossof- function (LoF) mutation, is a dominant approach for rice functional genomic studies. The objective of this study was to find out whether Transcriptional Activator-Facilitated Enhancer Trap (TAFET) T-DNA insertion inducing mutant phenotypes in rice TAFET population. Materials used in this experiment were T1 generation of 270 rice TAFET lines. Eight plants of e

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5

Koerniati, Sri. "UNTAGGED MUTATION IN RICE GAL4/VP16 TRANSCRIPTIONAL ACTIVATOR FACILITATED-ENHANCER TRAP LINES." Indonesian Journal of Agricultural Science 14, no. 1 (2013): 27. http://dx.doi.org/10.21082/ijas.v14n1.2013.p27-35.

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An enhancer trap system is an insertional mutagenesis based upon gene expression, instead of gene knock-out, so its insertion in genome is expected not linked to any dramatic changes in plant phenotypes. Gene knock-out, leading to lossof- function (LoF) mutation, is a dominant approach for rice functional genomic studies. The objective of this study was to find out whether Transcriptional Activator-Facilitated Enhancer Trap (TAFET) T-DNA insertion inducing mutant phenotypes in rice TAFET population. Materials used in this experiment were T1 generation of 270 rice TAFET lines. Eight plants of e

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6

Richardson, Kim, Sarah Fowler, Carly Pullen, Caryl Skelton, Bret Morris, and Jo Putterill. "T-DNA tagging of a flowering-time gene and improved gene transfer by in planta transformation of Arabidopsis." Functional Plant Biology 25, no. 1 (1998): 125. http://dx.doi.org/10.1071/pp97109.

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Gene tagging with insertional mutagens greatly facilitates the isolation of novel genes. A new collection of Arabidopsis T-DNA tag insertion lines (n=2165) was generated by in planta transformation. Whole plants were vacuum-infiltrated in a suspension of Agrobacterium carrying the pGKB5 tagging vector. The efficiency of transformation increased with addition of the surfactant Silwet L-77 (0.005% v/v) to the Agrobacterium suspension. Visual screens of the T-DNA lines identified two mutants with floral defects. Allelism tests suggested that a mutation in the GIGANTEA gene was responsible for the

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7

Martinelli, Laurie. "Australian Journal of Plant Physiology: Editorial report." Functional Plant Biology 25, no. 1 (1998): I. http://dx.doi.org/10.1071/ppv25n1_ed.

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Gene tagging with insertional mutagens greatly facilitates the isolation of novel genes. A new collection of Arabidopsis T-DNA tag insertion lines (n=2165) was generated by in planta transformation. Whole plants were vacuum-infiltrated in a suspension of Agrobacterium carrying the pGKB5 tagging vector. The efficiency of transformation increased with addition of the surfactant Silwet L-77 (0.005% v/v) to the Agrobacterium suspension. Visual screens of the T-DNA lines identified two mutants with floral defects. Allelism tests suggested that a mutation in the GIGANTEA gene was responsible for the

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8

Feldmann, K. A., M. D. Marks, M. L. Christianson, and R. S. Quatrano. "A Dwarf Mutant of Arabidopsis Generated by T-DNA Insertion Mutagenesis." Science 243, no. 4896 (1989): 1351–54. http://dx.doi.org/10.1126/science.243.4896.1351.

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9

Xu, Liangsheng, and Weidong Chen. "Random T-DNA Mutagenesis Identifies a Cu/Zn Superoxide Dismutase Gene as a Virulence Factor of Sclerotinia sclerotiorum." Molecular Plant-Microbe Interactions® 26, no. 4 (2013): 431–41. http://dx.doi.org/10.1094/mpmi-07-12-0177-r.

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Agrobacterium-mediated transformation (AMT) was used to identify potential virulence factors in Sclerotinia sclerotiorum. Screening AMT transformants identified two mutants showing significantly reduced virulence. The mutants showed growth rate, sclerotial formation, and oxalate production similar to that of the wild type. The mutation was due to a single T-DNA insertion at 212 bp downstream of the Cu/Zn superoxide dismutase (SOD) gene (SsSOD1, SS1G_00699). Expression levels of SsSOD1 were significantly increased under oxidative stresses or during plant infection in the wild-type strain but co

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10

Elliott, Candace E., and Barbara J. Howlett. "Overexpression of a 3-Ketoacyl-CoA Thiolase in Leptosphaeria maculans Causes Reduced Pathogenicity on Brassica napus." Molecular Plant-Microbe Interactions® 19, no. 6 (2006): 588–96. http://dx.doi.org/10.1094/mpmi-19-0588.

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Agrobacterium tumefaciens-mediated random mutagenesis was used to generate insertional mutants of the fungus Leptosphaeria maculans. Of 91 transformants screened, only one (A3) produced lesions of reduced size on cotyledons of canola (Brassica napus). Genes flanking the T-DNA insertion had the best matches to an alcohol dehydrogenase class 4 (ADH4)-like gene (Adh4L) and a 3-ketoacyl-CoA thiolase gene (Thiol) and were expressed in mutant A3 in vitro and in planta at significantly higher levels than in the wild type. This is the first report of a T-DNA insertion in fungi causing increased gene e

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11

Deng, Sheng, Cai-yue Wang, Xin Zhang, and Ling Lin. "Bidirectional promoter trapping T-DNA for insertional mutagenesis in Verticillium dahliae." Canadian Journal of Microbiology 60, no. 7 (2014): 445–54. http://dx.doi.org/10.1139/cjm-2014-0081.

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Transfer DNA (T-DNA)-based random insertional mutagenesis is a universal forward genetic approach for gene identification and cloning in many phytopathogenic fungi. In a large number of randomly selected transformants, screening for mutants with a specific phenotype is laborious, especially for pathogenicity-defective mutants. To accelerate mutant screening and gene identification, a bidirectional promoter-trapping Ti binary vector, 1300-bisGFP-hyg, was constructed and deployed in this study. More than 6000 Verticillium dahliae transformants were obtained by the mediation of Agrobacterium tume

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12

Idnurm, Alexander, Jennifer L. Reedy, Jesse C. Nussbaum, and Joseph Heitman. "Cryptococcus neoformans Virulence Gene Discovery through Insertional Mutagenesis." Eukaryotic Cell 3, no. 2 (2004): 420–29. http://dx.doi.org/10.1128/ec.3.2.420-429.2004.

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ABSTRACT Insertional mutagenesis was applied to Cryptococcus neoformans to identify genes associated with virulence attributes. Using biolistic transformation, we generated 4,300 nourseothricin (NAT)-resistant strains, of which 590 exhibited stable resistance. We focused on mutants with defects in established virulence factors and identified two with reduced growth at 37°C, four with reduced production of the antioxidant pigment melanin, and two with an increased sensitivity to nitric oxide (NO). The NAT insertion and mutant phenotypes were genetically linked in five of eight mutants, and the

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13

Howden, Ross, Soon Ki Park, James M. Moore, James Orme, Ueli Grossniklaus, and David Twell. "Selection of T-DNA-Tagged Male and Female Gametophytic Mutants by Segregation Distortion in Arabidopsis." Genetics 149, no. 2 (1998): 621–31. http://dx.doi.org/10.1093/genetics/149.2.621.

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Abstract As a strategy for the identification of T-DNA-tagged gametophytic mutants, we have used T-DNA insertional mutagenesis based on screening for distorted segregation ratios by antibiotic selection. Screening of ~1000 transgenic Arabidopsis families led to the isolation of eight lines showing reproducible segregation ratios of ~1:1, suggesting that these lines are putative gametophytic mutants caused by T-DNA insertion at a single locus. Genetic analysis of T-DNA transmission through reciprocal backcrosses with wild type showed severe reductions in genetic transmission of the T-DNA throug

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14

Budziszewski, Gregory J., Sharon Potter Lewis, Lyn Wegrich Glover, et al. "Arabidopsis Genes Essential for Seedling Viability: Isolation of Insertional Mutants and Molecular Cloning." Genetics 159, no. 4 (2001): 1765–78. http://dx.doi.org/10.1093/genetics/159.4.1765.

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Abstract We have undertaken a large-scale genetic screen to identify genes with a seedling-lethal mutant phenotype. From screening ~38,000 insertional mutant lines, we identified &gt;500 seedling-lethal mutants, completed cosegregation analysis of the insertion and the lethal phenotype for &gt;200 mutants, molecularly characterized 54 mutants, and provided a detailed description for 22 of them. Most of the seedling-lethal mutants seem to affect chloroplast function because they display altered pigmentation and affect genes encoding proteins predicted to have chloroplast localization. A

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15

Jia, Qi, Paul Bundock, Paul J. J. Hooykaas, and Sylvia de Pater. "Agrobacterium tumefaciens T-DNA Integration and Gene Targeting in Arabidopsis thaliana Non-Homologous End-Joining Mutants." Journal of Botany 2012 (February 29, 2012): 1–13. http://dx.doi.org/10.1155/2012/989272.

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In order to study the role of AtKu70 and AtKu80 in Agrobacterium-mediated transformation and gene targeting, plant lines with a T-DNA insertion in AtKu80 or AtKu70 genes were functionally characterized. Such plant lines lacked both subunits, indicating that heterodimer formation between AtKu70 and AtKu80 is needed for the stability of the proteins. Homozygous mutants were phenotypically indistinguishable from wild-type plants and were fertile. However, they were hypersensitive to the genotoxic agent bleomycin, resulting in more DSBs as quantified in comet assays. They had lower end-joining eff

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McElver, John, Iris Tzafrir, George Aux, et al. "Insertional Mutagenesis of Genes Required for Seed Development inArabidopsis thaliana." Genetics 159, no. 4 (2001): 1751–63. http://dx.doi.org/10.1093/genetics/159.4.1751.

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AbstractThe purpose of this project was to identify large numbers of Arabidopsis genes with essential functions during seed development. More than 120,000 T-DNA insertion lines were generated following Agrobacterium-mediated transformation. Transgenic plants were screened for defective seeds and putative mutants were subjected to detailed analysis in subsequent generations. Plasmid rescue and TAIL-PCR were used to recover plant sequences flanking insertion sites in tagged mutants. More than 4200 mutants with a wide range of seed phenotypes were identified. Over 1700 of these mutants were analy

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17

Chehab, E. Wassim, Se Kim, Tatyana Savchenko, Daniel Kliebenstein, Katayoon Dehesh, and Janet Braam. "Intronic T-DNA Insertion Renders Arabidopsis opr3 a Conditional Jasmonic Acid-Producing Mutant." Plant Physiology 156, no. 2 (2011): 770–78. http://dx.doi.org/10.1104/pp.111.174169.

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18

Procissi, Antonia, Solveig de Laissardière, Madina Férault, Daniel Vezon, Georges Pelletier, and Sandrine Bonhomme. "Five Gametophytic Mutations Affecting Pollen Development and Pollen Tube Growth in Arabidopsis thaliana." Genetics 158, no. 4 (2001): 1773–83. http://dx.doi.org/10.1093/genetics/158.4.1773.

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Abstract Mutant analysis represents one of the most reliable approaches to identifying genes involved in plant development. The screening of the Versailles collection of Arabidopsis thaliana T-DNA insertion transformants has allowed us to isolate different mutations affecting male gametophytic functions and viability. Among several mutated lines, five have been extensively studied at the genetic, molecular, and cytological levels. For each mutant, several generations of selfing and outcrossing have been carried out, leading to the conclusion that all these mutations are tagged and affect only

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19

Gao, Yangbin, Yi Zhang, Da Zhang, Xinhua Dai, Mark Estelle, and Yunde Zhao. "Auxin binding protein 1 (ABP1) is not required for either auxin signaling or Arabidopsis development." Proceedings of the National Academy of Sciences 112, no. 7 (2015): 2275–80. http://dx.doi.org/10.1073/pnas.1500365112.

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Auxin binding protein 1 (ABP1) has been studied for decades. It has been suggested that ABP1 functions as an auxin receptor and has an essential role in many developmental processes. Here we present our unexpected findings that ABP1 is neither required for auxin signaling nor necessary for plant development under normal growth conditions. We used our ribozyme-based CRISPR technology to generate an Arabidopsis abp1 mutant that contains a 5-bp deletion in the first exon of ABP1, which resulted in a frameshift and introduction of early stop codons. We also identified a T-DNA insertion abp1 allele

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20

Park, Hee-Yeon, Yang Qin, and Jae-Keun Sohn. "Physicochemical Properties of a Giant Embryo Mutant Induced by T-DNA Insertion in Rice." Korean Journal of Crop Science 56, no. 4 (2011): 413–19. http://dx.doi.org/10.7740/kjcs.2011.56.4.413.

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21

Piffanelli, Pietro, Gaétan Droc, Delphine Mieulet, et al. "Large-scale characterization of Tos17 insertion sites in a rice T-DNA mutant library." Plant Molecular Biology 65, no. 5 (2007): 587–601. http://dx.doi.org/10.1007/s11103-007-9222-3.

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22

Webb, K. Judith, Leif Skøt, Margaret N. Nicholson, Bodil Jørgensen, and Sue Mizen. "Mesorhizobium loti Increases Root-Specific Expression of a Calcium-Binding Protein Homologue Identified by Promoter Tagging in Lotus japonicus." Molecular Plant-Microbe Interactions® 13, no. 6 (2000): 606–16. http://dx.doi.org/10.1094/mpmi.2000.13.6.606.

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A promoter tagging program in the legume Lotus japonicus was initiated to identify plant genes involved in the nitrogen-fixing symbiosis between legumes and rhizobia. Seven transformed plant lines expressing the promoterless reporter gene uidA (β-glucuronidase; GUS) specifically in roots and/or nodules were identified. Four of these expressed GUS in the roots only after inoculation with nodule-forming Mesorhizobium loti. In one line (T90), GUS activity was found in the root epidermis, including root hairs. During seedling growth, GUS expression gradually became focused in developing nodules an

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Zhou, Xue-Rong, and Peter J. Christie. "Mutagenesis of the Agrobacterium VirE2 Single-Stranded DNA-Binding Protein Identifies Regions Required for Self-Association and Interaction with VirE1 and a Permissive Site for Hybrid Protein Construction." Journal of Bacteriology 181, no. 14 (1999): 4342–52. http://dx.doi.org/10.1128/jb.181.14.4342-4352.1999.

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ABSTRACT The VirE2 single-stranded DNA-binding protein (SSB) ofAgrobacterium tumefaciens is required for delivery of T-DNA to the nuclei of susceptible plant cells. By yeast two-hybrid and immunoprecipitation analyses, VirE2 was shown to self-associate and to interact with VirE1. VirE2 mutants with small deletions or insertions of a 31-residue oligopeptide (i31) at the N or C terminus or with an i31 peptide insertion at Leu236 retained the capacity to form homomultimers. By contrast, VirE2 mutants with modifications outside a central region located between residues 320 and 390 retained the cap

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Aklilu, Behailu B., Ryan S. Soderquist, and Kevin M. Culligan. "Genetic analysis of the Replication Protein A large subunit family in Arabidopsis reveals unique and overlapping roles in DNA repair, meiosis and DNA replication." Nucleic Acids Research 42, no. 5 (2013): 3104–18. http://dx.doi.org/10.1093/nar/gkt1292.

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Abstract Replication Protein A (RPA) is a heterotrimeric protein complex that binds single-stranded DNA. In plants, multiple genes encode the three RPA subunits (RPA1, RPA2 and RPA3), including five RPA1-like genes in Arabidopsis. Phylogenetic analysis suggests two distinct groups composed of RPA1A, RPA1C, RPA1E (ACE group) and RPA1B, RPA1D (BD group). ACE-group members are transcriptionally induced by ionizing radiation, while BD-group members show higher basal transcription and are not induced by ionizing radiation. Analysis of rpa1 T-DNA insertion mutants demonstrates that although each mut

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Qin, Xue, Jun Hua Liu, Wen Sheng Zhao, Xu Jun Chen, Ze Jian Guo, and You Liang Peng. "Gibberellin 20-Oxidase Gene OsGA20ox3 Regulates Plant Stature and Disease Development in Rice." Molecular Plant-Microbe Interactions® 26, no. 2 (2013): 227–39. http://dx.doi.org/10.1094/mpmi-05-12-0138-r.

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Gibberellin (GA) 20-oxidase (GA20ox) catalyses consecutive steps of oxidation in the late part of the GA biosynthetic pathway. A T-DNA insertion mutant (17S-14) in rice, with an elongated phenotype, was isolated. Analysis of the flanking sequences of the T-DNA insertion site revealed that an incomplete T-DNA integration resulted in enhanced constitutively expression of downstream OsGA20ox3 in the mutant. The accumulation of bioactive GA1 and GA4 were increased in the mutant in comparison with the wild-type plant. Transgenic plants overexpressing OsGA20ox3 showed phenotypes similar to those of

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Gutensohn, M., S. Pahnke, Ü. Kolukisaoglu, et al. "Characterization of a T-DNA insertion mutant for the protein import receptor atToc33 from chloroplasts." Molecular Genetics and Genomics 272, no. 4 (2004): 379–96. http://dx.doi.org/10.1007/s00438-004-1068-7.

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Guo, Guangming, and Bernard Weiss. "Endonuclease V (nfi) Mutant ofEscherichia coli K-12." Journal of Bacteriology 180, no. 1 (1998): 46–51. http://dx.doi.org/10.1128/jb.180.1.46-51.1998.

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ABSTRACT Endonuclease V (deoxyinosine 3′ endonuclease), the product of thenfi gene, has a specificity that encompasses DNAs containing dIMP, abasic sites, base mismatches, uracil, and even untreated single-stranded DNA. To determine its importance in DNA repair pathways, nfi insertion mutants and overproducers (strains bearing nfi plasmids) were constructed. The mutants displayed a twofold increase in spontaneous mutations for several markers and an increased sensitivity to killing by bleomycin and nitrofurantoin. An nfi mutation increased both cellular resistance to and mutability by nitrous

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Liu, Rundong, Wonyong Kim, Jaycee Augusto Paguirigan, Min-Hye Jeong, and Jae-Seoun Hur. "Establishment of Agrobacterium tumefaciens-Mediated Transformation of Cladonia macilenta, a Model Lichen-Forming Fungus." Journal of Fungi 7, no. 4 (2021): 252. http://dx.doi.org/10.3390/jof7040252.

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Despite the fascinating biology of lichens, such as the symbiotic association of lichen-forming fungi (mycobiont) with their photosynthetic partners and their ability to grow in harsh habitats, lack of genetic tools manipulating mycobiont has hindered studies on genetic mechanisms underpinning lichen biology. Thus, we established an Agrobacterium tumefaciens-mediated transformation (ATMT) system for genetic transformation of a mycobiont isolated from Cladonia macilenta. A set of combinations of ATMT conditions, such as input biomass of mycobiont, co-cultivation period with Agrobacterium cells,

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Zhao, Bo, Yongyan Tang, Baocai Zhang, et al. "The Temperature-Dependent Retention of Introns in GPI8 Transcripts Contributes to a Drooping and Fragile Shoot Phenotype in Rice." International Journal of Molecular Sciences 21, no. 1 (2019): 299. http://dx.doi.org/10.3390/ijms21010299.

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Attachment of glycosylphosphatidylinositols (GPIs) to the C-termini of proteins is one of the most common posttranslational modifications in eukaryotic cells. GPI8/PIG-K is the catalytic subunit of the GPI transamidase complex catalyzing the transfer en bloc GPI to proteins. In this study, a T-DNA insertional mutant of rice with temperature-dependent drooping and fragile (df) shoots phenotype was isolated. The insertion site of the T-DNA fragment was 879 bp downstream of the stop codon of the OsGPI8 gene, which caused introns retention in the gene transcripts, especially at higher temperatures

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Jithesh, Mundaya N., Owen S. D. Wally, Iain Manfield, Alan T. Critchley, David Hiltz, and Balakrishnan Prithiviraj. "Analysis of Seaweed Extract-induced Transcriptome Leads to Identification of a Negative Regulator of Salt Tolerance in Arabidopsis." HortScience 47, no. 6 (2012): 704–9. http://dx.doi.org/10.21273/hortsci.47.6.704.

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Successful development of plants resistant to salinity stress is problematic as a result of the complex polygenic natures of salt tolerance. Previously, alkaline extracts of the brown seaweed Ascophyllum nodosum have shown promise in enhancing plant tolerance toward abiotic stresses. To understand the underlying molecular mechanisms, the whole genome transcriptome of Arabidopsis undergoing salt stress was analyzed by microarray analysis after treatment with the chemical components of A. nodosum extracts (ANE). Treatment with ANE induced many positive regulators of salt tolerance in addition to

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Sadhukhan, Ayan, Takuo Enomoto, Yuriko Kobayashi, et al. "Sensitive to Proton Rhizotoxicity1 Regulates Salt and Drought Tolerance of Arabidopsis thaliana through Transcriptional Regulation of CIPK23." Plant and Cell Physiology 60, no. 9 (2019): 2113–26. http://dx.doi.org/10.1093/pcp/pcz120.

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Abstract The transcription factor sensitive to proton rhizotoxicity 1 (STOP1) regulates multiple stress tolerances. In this study, we confirmed its involvement in NaCl and drought tolerance. The root growth of the T-DNA insertion mutant of STOP1 (stop1) was sensitive to NaCl-containing solidified MS media. Transcriptome analysis of stop1 under NaCl stress revealed that STOP1 regulates several genes related to salt tolerance, including CIPK23. Among all available homozygous T-DNA insertion mutants of the genes suppressed in stop1, only cipk23 showed a NaCl-sensitive root growth phenotype compar

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Meco, Victoriano, Isabel Egea, Ana Ortíz-Atienza, et al. "The Salt Sensitivity Induced by Disruption of Cell Wall-Associated Kinase 1 (SlWAK1) Tomato Gene Is Linked to Altered Osmotic and Metabolic Homeostasis." International Journal of Molecular Sciences 21, no. 17 (2020): 6308. http://dx.doi.org/10.3390/ijms21176308.

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Tomato cell wall-associated kinase 1 (SlWAK1) has only been studied in biotic stress response and hence its function in abiotic stress remains unknown. In a screening under salinity of an insertional mutant collection of tomato (Solanum lycopersicum L.), a mutant exhibiting lower degree of leaf chlorosis than wild type (WT) together with reduced leaf Na+ accumulation was selected. Genetic analysis of the mutation revealed that a single T-DNA insertion in the SlWAK1 gene was responsible of the mutant phenotype. Slwak1 null mutant reduced its shoot growth compared with WT, despite its improved N

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Okubara, Patricia A., Rosa Arroyo-Garcia, Katherine A. Shen, et al. "A Transgenic Mutant of Lactuca sativa (Lettuce) with a T-DNA Tightly Linked to Loss of Downy Mildew Resistance." Molecular Plant-Microbe Interactions® 10, no. 8 (1997): 970–77. http://dx.doi.org/10.1094/mpmi.1997.10.8.970.

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One hundred and ninety-two independent primary transformants of lettuce cv. Diana were obtained by co-cultivation with Agrobacterium tumefaciens carrying constructs containing maize Ac transposase and Ds. R2 families were screened for mutations at four genes (Dm) for resistance to downy mildew. One family, designated dm3t524, had lost resistance to an isolate of Bremia lactucae expressing the avirulence gene Avr3. Loss of resistance segregated as a single recessive allele of Dm3. The mutation was not due to a large deletion as all molecular markers flanking Dm3 were present. Loss of Dm3 activi

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Wang, Yan, Klaas Bouwmeester, Patrick Beseh, Weixing Shan, and Francine Govers. "Phenotypic Analyses of Arabidopsis T-DNA Insertion Lines and Expression Profiling Reveal That Multiple L-Type Lectin Receptor Kinases Are Involved in Plant Immunity." Molecular Plant-Microbe Interactions® 27, no. 12 (2014): 1390–402. http://dx.doi.org/10.1094/mpmi-06-14-0191-r.

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L-type lectin receptor kinases (LecRK) are membrane-spanning receptor-like kinases with putative roles in biotic and abiotic stress responses and in plant development. In Arabidopsis, 45 LecRK were identified but their functions are largely unknown. Here, a systematic functional analysis was carried out by evaluating phenotypic changes of Arabidopsis LecRK T-DNA insertion lines in plant development and upon exposure to various external stimuli. None of the LecRK T-DNA insertion lines showed clear developmental changes, either under normal conditions or upon abiotic stress treatment. However, m

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Kim, Kang, Kim, An, and Paek. "OsWRKY5 Promotes Rice Leaf Senescence via Senescence-Associated NAC and Abscisic Acid Biosynthesis Pathway." International Journal of Molecular Sciences 20, no. 18 (2019): 4437. http://dx.doi.org/10.3390/ijms20184437.

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he onset of leaf senescence is triggered by external cues and internal factors such as phytohormones and signaling pathways involving transcription factors (TFs). Abscisic acid (ABA) strongly induces senescence and endogenous ABA levels are finely tuned by many senescence-associated TFs. Here, we report on the regulatory function of the senescence-induced TF OsWRKY5 TF in rice (Oryza sativa). OsWRKY5 expression was rapidly upregulated in senescing leaves, especially in yellowing sectors initiated by aging or dark treatment. A T-DNA insertion activation-tagged OsWRKY5-overexpressing mutant (ter

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Sarmiento-Villamil, Jorge L., Nicolás E. García-Pedrajas, M. Carmen Cañizares, and María D. García-Pedrajas. "Molecular Mechanisms Controlling the Disease Cycle in the Vascular Pathogen Verticillium dahliae Characterized Through Forward Genetics and Transcriptomics." Molecular Plant-Microbe Interactions® 33, no. 6 (2020): 825–41. http://dx.doi.org/10.1094/mpmi-08-19-0228-r.

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The soil-borne pathogen Verticillium dahliae has a worldwide distribution and a plethora of hosts of agronomic value. Molecular analysis of virulence processes can identify targets for disease control. In this work, we compared the global gene transcription profile of random T-DNA insertion mutant strain D-10-8F, which exhibits reduced virulence and alterations in microsclerotium formation and polar growth, with that of the wild-type strain. Three genes identified as differentially expressed were selected for functional characterization. To produce deletion mutants, we developed an updated ver

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Hamashima, Noriko, Xiaonan Xie, Mio Hikawa, Tomohiro Suzuki, and Yutaka Kodama. "A gain-of-function T-DNA insertion mutant of Marchantia polymorpha hyper-accumulates flavonoid riccionidin A." Plant Biotechnology 36, no. 3 (2019): 201–4. http://dx.doi.org/10.5511/plantbiotechnology.19.0722a.

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Khalilova, L. A., O. V. Sergienko, Yu V. Orlova, N. A. Myasoedov, I. V. Karpichev, and Yu V. Balnokin. "Arabidopsis thaliana Mutant with T-DNA Insertion in the Flot1 (At5g25250) Gene Promotor Possesses Increased Resistance to NaCl." Russian Journal of Plant Physiology 67, no. 2 (2020): 275–84. http://dx.doi.org/10.1134/s1021443720020077.

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Chern, Chyr-Guan, Ming-Jen Fan, Su-May Yu, et al. "A rice phenomics study—phenotype scoring and seed propagation of a T-DNA insertion-induced rice mutant population." Plant Molecular Biology 65, no. 4 (2007): 427–38. http://dx.doi.org/10.1007/s11103-007-9218-z.

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Ariizumi, K., H. Takahashi, M. Nakamura, and H. Ariga. "Effect of silencer on polyomavirus DNA replication." Molecular and Cellular Biology 9, no. 9 (1989): 4026–31. http://dx.doi.org/10.1128/mcb.9.9.4026.

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We have cloned the cellular sequence termed box DNA from the enhancer region of polyomavirus F9 mutant fPyF9. Box DNA functions as a negative transcriptional element (silencer) in undifferentiated F9 cells but not in differentiated L cells. Plasmid DNAs containing the origin and enhancer of polyomavirus were used to measure simultaneously transcriptional and replication activities in transfected cells. DNA replication activity was significantly reduced under conditions in which the silencer was able to reduce enhancer activity in F9 cells. On the other hand, when the silencer could not repress

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Ariizumi, K., H. Takahashi, M. Nakamura, and H. Ariga. "Effect of silencer on polyomavirus DNA replication." Molecular and Cellular Biology 9, no. 9 (1989): 4026–31. http://dx.doi.org/10.1128/mcb.9.9.4026-4031.1989.

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We have cloned the cellular sequence termed box DNA from the enhancer region of polyomavirus F9 mutant fPyF9. Box DNA functions as a negative transcriptional element (silencer) in undifferentiated F9 cells but not in differentiated L cells. Plasmid DNAs containing the origin and enhancer of polyomavirus were used to measure simultaneously transcriptional and replication activities in transfected cells. DNA replication activity was significantly reduced under conditions in which the silencer was able to reduce enhancer activity in F9 cells. On the other hand, when the silencer could not repress

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Nguyen Thi, Hong, Yoshikazu Tanaka, Tuyen Vo Thi Minh, and Ham Le Huy. "Identification of some nucleotide mutations in Waxy gene (BGIOSGA022241) of a mutant rice line." Nuclear Science and Technology 8, no. 3 (2021): 36–52. http://dx.doi.org/10.53747/jnst.v8i3.72.

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Waxy genes of the original variety and its mutant type were sequenced by Sanger method and compared through Nucleotide Basic Local Alignment Search Tool (BLASTN) to clarify differences. BLASTN result showed four nucleotide mutations in coding regions and 59 nucleotide mutations in noncoding regions. Four point mutations in coding regions were: the deletion of T/- at position 34 and the insertion of -/T between positions 70 and 71 in exon 3; the substitution of C/T at position 14 in exon 4 and the substitution of T/C at position 115 in exon 9. In 59 mutant nucleotides in non-coding regions, som

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Zou, Baohong, Zhenhua Jia, Shuangmei Tian, et al. "AtMYB44 positively modulates disease resistance to Pseudomonas syringae through the salicylic acid signalling pathway in Arabidopsis." Functional Plant Biology 40, no. 3 (2013): 304. http://dx.doi.org/10.1071/fp12253.

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Plant MYB transcription factors are implicated in resistance to biotic and abiotic stresses. Here, we demonstrate that an R2-R3 MYB transcription factor, AtMYB44, plays a role in the plant defence response to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (PstDC3000). The expression of AtMYB44 was upregulated upon pathogen infection and treatments with defence-related phytohormones. Transgenic plants overexpressing AtMYB44 (35S-Ms) exhibited greater levels of PR1 gene expression, cell death, callose deposition and hydrogen peroxide (H2O2) accumulation in leaves infected with Pst

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Negruk, Valentin, Galina Eisner, and Bertrand Lemieux. "Addition–deletion mutations in transgenic Arabidopsis thaliana generated by the seed co-cultivation method." Genome 39, no. 6 (1996): 1117–22. http://dx.doi.org/10.1139/g96-140.

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We have characterized three mutant alleles of the CER2 gene of Arabidopsis thaliana by sequencing polymerase chain reaction products of this gene generated with template DNA isolated from lines MK1, BRL7, and BRL17. Sequence analysis of the amplification product from line BRL17 revealed a 17-bp deletion in the CER2 gene. The CER2 gene of BRL7 differs from the wild-type sequence by a 2-base substitution and 2-base insertion. As both of these lines were isolated from the transformant populations generated by Dr. K. Feldmann and his collaborators, we suggest that these small rearrangements may be

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Bechtold, Nicole, Bénédicte Jaudeau, Sylvie Jolivet, et al. "The Maternal Chromosome Set Is the Target of the T-DNA in the in Planta Transformation of Arabidopsis thaliana." Genetics 155, no. 4 (2000): 1875–87. http://dx.doi.org/10.1093/genetics/155.4.1875.

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Abstract In planta transformation methods are now commonly used to transform Arabidopsis thaliana by Agrobacterium tumefaciens. The origin of transformants obtained by these methods has been studied by inoculating different floral stages and examining gametophytic expression of an introduced β-glucuronidase marker gene encoding GUS. We observed that transformation can still occur after treating flowers where embryo sacs have reached the stage of the third division. No GUS expression was observed in embryo sacs or pollen of plants infiltrated with an Agrobacterium strain bearing a GUS gene unde

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Garcia, Nelson, Yubin Li, Hugo K. Dooner, and Joachim Messing. "Maize defective kernel mutant generated by insertion of a Ds element in a gene encoding a highly conserved TTI2 cochaperone." Proceedings of the National Academy of Sciences 114, no. 20 (2017): 5165–70. http://dx.doi.org/10.1073/pnas.1703498114.

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We have used the newly engineered transposable element Dsg to tag a gene that gives rise to a defective kernel (dek) phenotype. Dsg requires the autonomous element Ac for transposition. Upon excision, it leaves a short DNA footprint that can create in-frame and frameshift insertions in coding sequences. Therefore, we could create alleles of the tagged gene that confirmed causation of the dek phenotype by the Dsg insertion. The mutation, designated dek38-Dsg, is embryonic lethal, has a defective basal endosperm transfer (BETL) layer, and results in a smaller seed with highly underdeveloped endo

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Moseler, Anna, Isabel Aller, Stephan Wagner, et al. "The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana." Proceedings of the National Academy of Sciences 112, no. 44 (2015): 13735–40. http://dx.doi.org/10.1073/pnas.1510835112.

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The iron-sulfur cluster (ISC) is an ancient and essential cofactor of many proteins involved in electron transfer and metabolic reactions. In Arabidopsis, three pathways exist for the maturation of iron-sulfur proteins in the cytosol, plastids, and mitochondria. We functionally characterized the role of mitochondrial glutaredoxin S15 (GRXS15) in biogenesis of ISC containing aconitase through a combination of genetic, physiological, and biochemical approaches. Two Arabidopsis T-DNA insertion mutants were identified as null mutants with early embryonic lethal phenotypes that could be rescued by

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Li, Fuzhen, Guocheng Hu, Yaping Fu, Huamin Si, Xuemei Bai, and Zongxiu Sun. "Genetic analysis and high-resolution mapping of a premature senescence gene Pse(t) in rice (Oryza sativa L.)." Genome 48, no. 4 (2005): 738–46. http://dx.doi.org/10.1139/g05-030.

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A rice mutant, designated pse(t) (premature senescence, tentatively), was isolated from a T-DNA-inserted transgenic population. Senescence advanced more markedly in pse(t) than in wild-type ('Zhonghua 11', japonica) plants. Genetic analysis of pse(t) revealed that the premature senescence mutation was controlled by a single recessive nuclear gene, but that it was not induced by T-DNA insertion. In an effort to understand the genetic and molecular basis underlying premature senescence in rice, a map-based cloning strategy was used to localize Pse(t). High-resolution mapping of the Pse(t) locus

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Egert, Aurélie, Shaun Peters, Christelle Guyot, Bruno Stieger, and Felix Keller. "An Arabidopsis T-DNA Insertion Mutant for Galactokinase (AtGALK, At3g06580) Hyperaccumulates Free Galactose and is Insensitive to Exogenous Galactose." Plant and Cell Physiology 53, no. 5 (2012): 921–29. http://dx.doi.org/10.1093/pcp/pcs036.

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Chang, Yuxiao, Tuan Long, and Changyin Wu. "Effort and Contribution of T-DNA Insertion Mutant Library for Rice Functional Genomics Research in China: Review and Perspective." Journal of Integrative Plant Biology 54, no. 12 (2012): 953–66. http://dx.doi.org/10.1111/j.1744-7909.2012.01171.x.

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