Relevant bibliographies by topics / T-RFLP Analysis

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'T-RFLP Analysis'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'T-RFLP Analysis.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "T-RFLP Analysis"

1

Lueders, Tillmann, and Michael W. Friedrich. "Evaluation of PCR Amplification Bias by Terminal Restriction Fragment Length Polymorphism Analysis of Small-Subunit rRNA and mcrA Genes by Using Defined Template Mixtures of Methanogenic Pure Cultures and Soil DNA Extracts." Applied and Environmental Microbiology 69, no. 1 (2003): 320–26. http://dx.doi.org/10.1128/aem.69.1.320-326.2003.

Full text
Abstract:
ABSTRACT Terminal restriction fragment length polymorphism (T-RFLP) analysis is a widely used method for profiling microbial community structure in different habitats by targeting small-subunit (SSU) rRNA and also functional marker genes. It is not known, however, whether relative gene frequencies of individual community members are adequately represented in post-PCR amplicon frequencies as shown by T-RFLP. In this study, precisely defined artificial template mixtures containing genomic DNA of four different methanogens in various ratios were prepared for subsequent T-RFLP analysis. PCR amplic
APA, Harvard, Vancouver, ISO, and other styles
2

Jackson, Colin J., Richard C. Barton, and E. Glyn V. Evans. "Species Identification and Strain Differentiation of Dermatophyte Fungi by Analysis of Ribosomal-DNA Intergenic Spacer Regions." Journal of Clinical Microbiology 37, no. 4 (1999): 931–36. http://dx.doi.org/10.1128/jcm.37.4.931-936.1999.

Full text
Abstract:
Restriction fragment length polymorphisms (RFLPs) identified in the ribosomal-DNA (rDNA) repeat were used for molecular strain differentiation of the dermatophyte fungus Trichophyton rubrum. The polymorphisms were detected by hybridization ofEcoRI-digested T. rubrum genomic DNAs with a probe amplified from the small-subunit (18S) rDNA and adjacent internal transcribed spacer (ITS) regions. The rDNA RFLPs mapped to the nontranscribed spacer (NTS) region of the rDNA repeat and appeared similar to those caused by short repetitive sequences in the intergenic spacers of other fungi. Fourteen indivi
APA, Harvard, Vancouver, ISO, and other styles
3

Nagashima, Koji, Takayoshi Hisada, Maremi Sato, and Jun Mochizuki. "Application of New Primer-Enzyme Combinations to Terminal Restriction Fragment Length Polymorphism Profiling of Bacterial Populations in Human Feces." Applied and Environmental Microbiology 69, no. 2 (2003): 1251–62. http://dx.doi.org/10.1128/aem.69.2.1251-1262.2003.

Full text
Abstract:
ABSTRACT New primer-enzyme combinations for terminal restriction fragment length polymorphism (T-RFLP) targeting of the 16S rRNA gene were constructed by using the T-RFLP analysis program (designated TAP T-RFLP) located at the Ribosomal Database Project website, and their performance was examined empirically. By using the fluorescently labeled 516f primer (Escherichia coli positions 516 to 532) and 1510r primer (positions 1510 to 1492), the 16S rRNA gene was amplified from human fecal DNA. The resulting amplified product was digested with RsaI plus BfaI or with BslI. When the T-RFLP was carrie
APA, Harvard, Vancouver, ISO, and other styles
4

Egert, Markus, and Michael W. Friedrich. "Formation of Pseudo-Terminal Restriction Fragments, a PCR-Related Bias Affecting Terminal Restriction Fragment Length Polymorphism Analysis of Microbial Community Structure." Applied and Environmental Microbiology 69, no. 5 (2003): 2555–62. http://dx.doi.org/10.1128/aem.69.5.2555-2562.2003.

Full text
Abstract:
ABSTRACT Terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR-amplified genes is a widely used fingerprinting technique in molecular microbial ecology. In this study, we show that besides expected terminal restriction fragments (T-RFs), additional secondary T-RFs occur in T-RFLP analysis of amplicons from cloned 16S rRNA genes at high frequency. A total of 50% of 109 bacterial and 78% of 68 archaeal clones from the guts of cetoniid beetle larvae, using MspI and AluI as restriction enzymes, respectively, were affected by the presence of these additional T-RFs. These peaks
APA, Harvard, Vancouver, ISO, and other styles
5

Danovaro, R., G. M. Luna, A. Dell'Anno, and B. Pietrangeli. "Comparison of Two Fingerprinting Techniques, Terminal Restriction Fragment Length Polymorphism and Automated Ribosomal Intergenic Spacer Analysis, for Determination of Bacterial Diversity in Aquatic Environments." Applied and Environmental Microbiology 72, no. 9 (2006): 5982–89. http://dx.doi.org/10.1128/aem.01361-06.

Full text
Abstract:
ABSTRACT We investigated bacterial diversity in different aquatic environments (including marine and lagoon sediments, coastal seawater, and groundwater), and we compared two fingerprinting techniques (terminal restriction fragment length polymorphism [T-RFLP] and automated ribosomal intergenic spacer analysis [ARISA]) which are currently utilized for estimating richness and community composition. Bacterial diversity ranged from 27 to 99 phylotypes (on average, 56) using the T-RFLP approach and from 62 to 101 genotypes (on average, 81) when the same samples were analyzed using ARISA. The total
APA, Harvard, Vancouver, ISO, and other styles
6

Seldin, M. F., H. C. Morse, J. P. Reeves, C. L. Scribner, R. C. LeBoeuf, and A. D. Steinberg. "Genetic analysis of autoimmune gld mice. I. Identification of a restriction fragment length polymorphism closely linked to the gld mutation within a conserved linkage group." Journal of Experimental Medicine 167, no. 2 (1988): 688–93. http://dx.doi.org/10.1084/jem.167.2.688.

Full text
Abstract:
A linkage map of distal mouse chromosome 1 was generated using restriction fragment length polymorphism (RFLP) analysis of DNA prepared from 95 [C3H-gld/gld X Mus spretus)F1 X C3H-gld/gld] backcross mice. The gene order was: (centromere) C4bp, Ren-1,2, Ly-5, [At-3/gld], Apoa-2/Ly-17, Spna-1 (telomere). All mice expressing the phenotype of gld homozygotes were homozygous for the At-3 RFLP characteristic of C3H mice and none of the mice heterozygous for At-3 RFLPs had characteristics of gld homozygotes, demonstrating close linkage between these genes. The identification of an RFLP closely linked
APA, Harvard, Vancouver, ISO, and other styles
7

Junier, Pilar, Thomas Junier, and Karl-Paul Witzel. "TRiFLe, a Program for In Silico Terminal Restriction Fragment Length Polymorphism Analysis with User-Defined Sequence Sets." Applied and Environmental Microbiology 74, no. 20 (2008): 6452–56. http://dx.doi.org/10.1128/aem.01394-08.

Full text
Abstract:
ABSTRACT We describe TRiFLe, a freely accessible computer program that generates theoretical terminal restriction fragments (T-RFs) from any user-supplied sequence set tailored to a particular group of organisms, sequences from clone libraries, or sequences from specific genes. The program allows a rapid identification of the most polymorphic enzymes, creates a collection of T-RFs for the data set, and can potentially identify specific T-RFs in T-RF length polymorphism (T-RFLP) patterns by comparing theoretical and experimental results. TRiFLE was used for analyzing T-RFLP data generated for t
APA, Harvard, Vancouver, ISO, and other styles
8

Hartmann, Martin, and Franco Widmer. "Community Structure Analyses Are More Sensitive to Differences in Soil Bacterial Communities than Anonymous Diversity Indices." Applied and Environmental Microbiology 72, no. 12 (2006): 7804–12. http://dx.doi.org/10.1128/aem.01464-06.

Full text
Abstract:
ABSTRACT Changes in the diversity and structure of soil microbial communities may offer a key to understanding the impact of environmental factors on soil quality in agriculturally managed systems. Twenty-five years of biodynamic, bio-organic, or conventional management in the DOK long-term experiment in Switzerland significantly altered soil bacterial community structures, as assessed by terminal restriction fragment length polymorphism (T-RFLP) analysis. To evaluate these results, the relation between bacterial diversity and bacterial community structures and their discrimination potential w
APA, Harvard, Vancouver, ISO, and other styles
9

ASHER, A. J., L. S. WALDRON, and M. L. POWER. "Rapid identification of Giardia duodenalis assemblages in NSW using terminal-restriction fragment length polymorphism." Parasitology 139, no. 8 (2012): 1005–13. http://dx.doi.org/10.1017/s0031182012000388.

Full text
Abstract:
SUMMARYHumans are infected by 2 genetic assemblages (A and B) of Giardia duodenalis, a protozoan parasite that causes gastro-intestinal disease. Sub-assemblages AI, AII, BIII and BIV are commonly identified in human cases. Detection requires amplification of G. duodenalis loci. Subsequent DNA sequencing or restriction fragment length polymorphism (RFLP) identifies sub-assemblages but is expensive (DNA sequencing) or insensitive (RFLP). This study investigated a fluorescence-based detection method, using terminal-restriction fragment length polymorphism (T-RFLP) of the glutamate dehydrogenase g
APA, Harvard, Vancouver, ISO, and other styles
10

Wang, Jia Jia, Yu Mei Li, Zhi Wen Zhao, et al. "T-RFLP Analysis of Soil Microbial Community from Shandong Province for Forensic Science." Advanced Materials Research 599 (November 2012): 250–53. http://dx.doi.org/10.4028/www.scientific.net/amr.599.250.

Full text
Abstract:
Soil microbial community very little had been found as trace evidence， however， it could be vital for forensic science. In this paper, soil microbial community diversities from seventeen different regions of Shandong province were investigated by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The results of diversity index analysis show the highest Margalef index and Eveness index are 4.609 for sample D and 0.970 for sample E, respectively. In T-RFLP profile, the absolute advantage peak are 488bp (62.6% ) from sample O and 496.5bp (20.8%) from Sample H. The analysed terminal restr
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "T-RFLP Analysis"

1

Gustafson, Aubree Marie. "T-RFLP analysis of bacterial 16S rRNA gene sequences isolated from river otter (Lontra canadensis) scat and parasite screening for the presence of Toxoplasma gondii." Scholarly Commons, 2009. https://scholarlycommons.pacific.edu/uop_etds/732.

Full text
Abstract:
In order to analyze the bacterial community of river otter scat (fecal material) at the class level, river otter scat samples were collected from Grizzly Island Wildlife Area (Solano County, CA) and the Cosumnes River Preserve (Sacramento County, CA). DNA was isolated from each sample with the MOBIO PowerSoil™ DNA Isolation Kit and 16S rRNA gene sequences were amplified from each sample. After digestion with Mspl, TRFLPs were analyzed in an ABI Prism™ 310 Genetic Analyzetin triplicate and data peak information from each electropherogram was uploaded into the Phylogenetic Assignment Tool (PAT).
APA, Harvard, Vancouver, ISO, and other styles
2

Benitez, Maria Soledad. "Applied T-RFLP Analyses for the Identification and Characterization of Microbial Populations Associated With Damping-Off Incidence in a Transitional Organic Cropping System." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1218471106.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Kirker, Grant Terral. "EFFECTS OF CHLOROTHALONIL AND BUTYLATED HYDROXYTOLUENE ON MICROBIAL COMMUNITIES INVOLVED IN THE DETERIORATION OF WOOD USING TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM (T-RFLP) ANALYSES." MSSTATE, 2008. http://sun.library.msstate.edu/ETD-db/theses/available/etd-04022008-155301/.

Full text
Abstract:
The effects of an organic biocide (CTN) with and without co-added antioxidant (BHT) on microbial communities in SYP were assessed using terminal restriction fragment length polymorphism (T-RFLP) analyses in both field and accelerated decay laboratory studies. Ammoniacal copper quaternary (ACQ-C) was used as a positive control in the field study component, but not in the laboratory test. Field stakes were treated with 0.25 and 0.37% ammoniacal copper quat (ACQ-C), CTN (0.1 and 0.25%), CTN (0.1 and 0.25%) with 2% BHT added, 2% BHT alone, and controls were left untreated. In the field studies, pr
APA, Harvard, Vancouver, ISO, and other styles
4

Bleul, Catrin. "Molekularbiologische Analyse mikrobieller Gemeinschaften in Talsperrensedimenten." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2004. http://nbn-resolving.de/urn:nbn:de:swb:14-1097570982718-83940.

Full text
Abstract:
Mikrobielle Prozesse spielen eine wichtige Rolle im Sediment von Talsperren und Seen. Demgegenüber stehen nur unzureichende Erkenntnisse über die Zusammensetzung mikrobieller Biozönosen in Sedimenten sowie deren Aktivität zur Verfügung. Das Ziel dieser Studie war die Untersuchung und der Vergleich der Zusammensetzung und der Struktur mikrobieller Gemeinschaften in Sedimenten um eine Abschätzung der mikrobiellen Diversität in Talsperrensedimenten unterschiedlicher Trophie zu erreichen. Durch die Kombination der in dieser Arbeit verwendeten Methoden (Vergleichende 16S rDNA Analyse, Fingerprintte
APA, Harvard, Vancouver, ISO, and other styles
5

Eschenhagen, Martin. "Molekulare Untersuchung zweier Belebtschlammanlagen unter besonderer Berücksichtigung der biologischen Phosphorelimination." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2004. http://nbn-resolving.de/urn:nbn:de:swb:14-1091188675328-95596.

Full text
Abstract:
Aufgrund der ökologischen und ökonomischen Problematik der chemischen Phosphatfällung ist eine Optimierung der Effizienz und Stabilität der biologischen Verfahren zur Phosphat-elimination erforderlich. Hierfür ist jedoch ein fundiertes Wissen über die daran beteiligten Organismen eine entscheidende Vorraussetzung. Das Ziel der vorliegenden Arbeit war es, die mikrobielle Populationstruktur von zwei Belebtschlamm-anlagen im Labormaßstab mit Hilfe von drei unterschiedlichen 16S rDNA basierenden molekular-biologischen Methoden zu charakterisieren. Ein besonderer Schwer-punkt ist hierbei die Analys
APA, Harvard, Vancouver, ISO, and other styles
6

Kirker, Grant Terral. "Effects of chlorothalonil (CTN) and butylated hydroxy-toluene (BHT) on microbial communities involved in the deterioration of wood using terminal restriction fragment length polymorphism (T-RFLP) analyses." Diss., Mississippi State : Mississippi State University, 2008. http://library.msstate.edu/etd/show.asp?etd=etd-04022008-155301.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Eschenhagen, Martin. "Molekulare Untersuchung zweier Belebtschlammanlagen unter besonderer Berücksichtigung der biologischen Phosphorelimination." Doctoral thesis, Technische Universität Dresden, 2003. https://tud.qucosa.de/id/qucosa%3A24360.

Full text
Abstract:
Aufgrund der ökologischen und ökonomischen Problematik der chemischen Phosphatfällung ist eine Optimierung der Effizienz und Stabilität der biologischen Verfahren zur Phosphat-elimination erforderlich. Hierfür ist jedoch ein fundiertes Wissen über die daran beteiligten Organismen eine entscheidende Vorraussetzung. Das Ziel der vorliegenden Arbeit war es, die mikrobielle Populationstruktur von zwei Belebtschlamm-anlagen im Labormaßstab mit Hilfe von drei unterschiedlichen 16S rDNA basierenden molekular-biologischen Methoden zu charakterisieren. Ein besonderer Schwer-punkt ist hierbei die Analys
APA, Harvard, Vancouver, ISO, and other styles
8

Lenz, Erin Jennifer. "Rhizobial T-RFLP analysis for differentiating soils and habitats." Diss., 2008. http://proquest.umi.com/pqdweb?did=1606926031&sid=2&Fmt=2&clientId=3552&RQT=309&VName=PQD.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Lashaway, Aubrey Rain. "Water quality and eukaryotic plankton dynamics in the Mission-Aransas Estuary, Texas from 2011-2012." 2013. http://hdl.handle.net/2152/22101.

Full text
Abstract:
As the base of the food chain, plankton affect the cycling of nutrients and organic matter within ecosystems and support production at higher trophic levels. The overall goal of this project was to examine how natural water quality fluctuations, such as changes in nutrients, temperature, and salinity, influence estuarine plankton community structure. To achieve this, I examined water quality as well as the diversity and biomass of eukaryotic plankton communities in a subtropical estuary located within the Mission-Aransas National Estuarine Research Reserve. The sampling sites included in th
APA, Harvard, Vancouver, ISO, and other styles
10

Kernaghan, Shaun. "Characterization of the Bacterial Communities of the Tonsil of the Soft Palate of Swine." Thesis, 2013. http://hdl.handle.net/10214/7750.

Full text
Abstract:
Terminal restriction fragment length polymorphism (T-RFLP) analysis and pyrosequencing were used to characterize the microbiota of the tonsil of the soft palate of 126 unfit and 18 healthy pigs. The T-RFLP analysis method was first optimized for the study of the pig tonsil microbiota and the data compared with culture-based identification of common pig pathogens. Putative identifications of the members of the microbiota revealed that the phyla Firmicutes, Proteobacteria and Bacteroidetes were the most prevalent. A comparison of the T-RFLP analysis results grouped into clusters to clinical cond
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "T-RFLP Analysis"

1

Park, Min Sik, Aida Barbetti, Toshinao Takenouchi, and Paul I. Terasaki. "Analysis of Class II RFLP Patterns and T-Cell Clone Reactions." In Immunobiology of HLA. Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_267.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Kim, Sang-Hoon, and Terence Marsh. "Section 3 update: The Analysis of Microbial Communities with Terminal Restriction Fragment Length Polymorphism (T-RFLP)." In Molecular Microbial Ecology Manual. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-2177-0_315.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Tiquia, Sonia M. "Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Assess Microbial Community Structure in Compost Systems." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-439-5_6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

"Archaeal Diversity Analysis Using 16S rDNA T-RFLP (Terminal-Restriction Fragment Length Polymorphisms)." In Methods for the Study of Deep-Sea Sediments, Their Functioning and Biodiversity. CRC Press, 2009. http://dx.doi.org/10.1201/9781439811382-c21.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Li, J., Y. Zhou, Y. Zhuang, and J. Zhao. "Community structure of methanotrophs under wetland evolution of WuLiangSuHailake by T-RFLP analysis." In Frontiers of Energy and Environmental Engineering. CRC Press, 2012. http://dx.doi.org/10.1201/b13718-183.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

"2Chapter 1 Archaeal Diversity Analysis Using 16S rDNA T-RFLP (Terminal-Restriction Fragment Length Polymorphisms)." In Methods for the Study of Deep-Sea Sediments, Their Functioning and Biodiversity. CRC Press, 2009. http://dx.doi.org/10.1201/9781439811382-31.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "T-RFLP Analysis"

1

Dong, Ping, Yujiao Sun, Hongqi Wang, Liding Chen, and Hui Zhang. "Notice of Retraction: Study on Riparian Zone Microbial Community Structure of the Wenyu River by T-RFLP Analysis." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780057.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Bernaedi, F., V. Bertagnolo, S. Bartolai, L. Rossi, F. Panicucci, and F. Conconi. "A POINT MUTATION AND A GENE DELETION OF FVIII GENE IN SEVERE HAEMOPHILIA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644047.

Full text
Abstract:
The presence of Factor VIII (FVIII) gene lesions has been investigated in 100 haemophilia A patients using cDNA probes for the 3'part of FVIII gene (exons 14-26 ).In two related severe patients without inhibitor a deletion removesthe exon 26; the gene lesion has been confirmed with several restriction enzymes and has been shown by densitometry of the autoradiographic pattern in a woman of the same family. The complete deletionof the exon 26 has been described by Gitschier et al. in a patient with inhibitor. Thus the comparison of the end points of the two deletions could help to define the mec
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'T-RFLP Analysis' for particular source types:
Journal articles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography