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1

Soleimani, Vahab D., Gareth A. Palidwor, Parameswaran Ramachandran, Theodore J. Perkins, and Michael A. Rudnicki. "Chromatin tandem affinity purification sequencing." Nature Protocols 8, no. 8 (2013): 1525–34. http://dx.doi.org/10.1038/nprot.2013.088.

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2

Maine, G. N., N. Gluck, I. W. Zaidi, and E. Burstein. "Bimolecular Affinity Purification (BAP): Tandem Affinity Purification Using Two Protein Baits." Cold Spring Harbor Protocols 2009, no. 11 (2009): pdb.prot5318. http://dx.doi.org/10.1101/pdb.prot5318.

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3

Amberg, David C., Daniel J. Burke, and Jeffrey N. Strathern. "Tandem Affinity Protein (TAP) Purification from Yeast." Cold Spring Harbor Protocols 2006, no. 1 (2006): pdb.prot4153. http://dx.doi.org/10.1101/pdb.prot4153.

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4

Li, Yifeng. "The tandem affinity purification technology: an overview." Biotechnology Letters 33, no. 8 (2011): 1487–99. http://dx.doi.org/10.1007/s10529-011-0592-x.

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5

Van Leene, Jelle, Erwin Witters, Dirk Inzé, and Geert De Jaeger. "Boosting tandem affinity purification of plant protein complexes." Trends in Plant Science 13, no. 10 (2008): 517–20. http://dx.doi.org/10.1016/j.tplants.2008.08.002.

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6

Li, Yifeng. "Commonly used tag combinations for tandem affinity purification." Biotechnology and Applied Biochemistry 55, no. 2 (2010): 73–83. http://dx.doi.org/10.1042/ba20090273.

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7

Mishra, Vibhor. "Affinity Tags for Protein Purification." Current Protein & Peptide Science 21, no. 8 (2020): 821–30. http://dx.doi.org/10.2174/1389203721666200606220109.

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The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some affinity tags also serve a dual purpose as solubility enhancers for challenging protein targets. By applying a combinatorial approach, carefully chosen affinity tags designed in tandem have proven to be very successful in the purification of single proteins or multi-protein complexes. In this mini-review, the key features of the most commonly used affinity tags are discussed. The affinity tags have been classified into
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8

GOULD, K. "Tandem affinity purification and identification of protein complex components." Methods 33, no. 3 (2004): 239–44. http://dx.doi.org/10.1016/j.ymeth.2003.11.019.

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9

Stingl, Kerstin, Kristine Schauer, Chantal Ecobichon, et al. "In VivoInteractome ofHelicobacter pyloriUrease Revealed by Tandem Affinity Purification." Molecular & Cellular Proteomics 7, no. 12 (2008): 2429–41. http://dx.doi.org/10.1074/mcp.m800160-mcp200.

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10

Kaneko, Aki, Takashi Umeyama, Nozomu Hanaoka, Brian C. Monk, Yoshimasa Uehara, and Masakazu Niimi. "Tandem affinity purification of theCandida albicans septin protein complex." Yeast 21, no. 12 (2004): 1025–33. http://dx.doi.org/10.1002/yea.1147.

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11

Zanon, Alessandra, Aleksandar Rakovic, Hagen Blankenburg, et al. "Profiling of Parkin-Binding Partners Using Tandem Affinity Purification." PLoS ONE 8, no. 11 (2013): e78648. http://dx.doi.org/10.1371/journal.pone.0078648.

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12

Nonne, Nora, Maya Ameyar-Zazoua, Mouloud Souidi, and Annick Harel-Bellan. "Tandem affinity purification of miRNA target mRNAs (TAP-Tar)." Nucleic Acids Research 38, no. 4 (2009): e20-e20. http://dx.doi.org/10.1093/nar/gkp1100.

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13

Cox, D. M., M. Du, X. Guo, K. W. M. Siu, and J. C. McDermott. "Tandem Affinity Purification of Protein Complexes from Mammalian Cells." BioTechniques 33, no. 2 (2002): 267–70. http://dx.doi.org/10.2144/02332bm02.

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14

Puig, Oscar, Friederike Caspary, Guillaume Rigaut, et al. "The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex Purification." Methods 24, no. 3 (2001): 218–29. http://dx.doi.org/10.1006/meth.2001.1183.

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15

Yang, Xiao, Geoff P. Doherty, and Peter J. Lewis. "Tandem affinity purification vectors for use in gram positive bacteria." Plasmid 59, no. 1 (2008): 54–62. http://dx.doi.org/10.1016/j.plasmid.2007.11.001.

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16

Bartoi, Tudor, Kristoffer T. G. Rigbolt, Dan Du, Georg Köhr, Blagoy Blagoev, and Hans-Christian Kornau. "GABABReceptor Constituents Revealed by Tandem Affinity Purification from Transgenic Mice." Journal of Biological Chemistry 285, no. 27 (2010): 20625–33. http://dx.doi.org/10.1074/jbc.m109.049700.

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17

Cipak, Lubos, Mario Spirek, Maria Novatchkova, et al. "An improved strategy for tandem affinity purification-tagging ofSchizosaccharomyces pombegenes." PROTEOMICS 9, no. 20 (2009): 4825–28. http://dx.doi.org/10.1002/pmic.200800948.

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18

Veraksa, Alexey, Andreas Bauer, and Spyros Artavanis-Tsakonas. "Analyzing protein complexes inDrosophila with tandem affinity purification-mass spectrometry." Developmental Dynamics 232, no. 3 (2005): 827–34. http://dx.doi.org/10.1002/dvdy.20272.

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19

Henry, Scott M., Elissa Sutlief, Oscar Salas-Solano, and John Valliere-Douglass. "ELISA reagent coverage evaluation by affinity purification tandem mass spectrometry." mAbs 9, no. 7 (2017): 1065–75. http://dx.doi.org/10.1080/19420862.2017.1349586.

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20

Cheong, Clara, Patricia Lon Ng, Rhonda Ponnampalam, Heng-Hang Tsai, Guillaume Bourque, and Thomas Lufkin. "In silico tandem affinity purification refines an Oct4 interaction list." Stem Cell Research & Therapy 2, no. 3 (2011): 26. http://dx.doi.org/10.1186/scrt67.

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21

Ostrowski, Jerzy, and Karol Bomsztyk. "Purification of DNA-binding proteins using tandem DNA- affinity column." Nucleic Acids Research 21, no. 4 (1993): 1045–46. http://dx.doi.org/10.1093/nar/21.4.1045.

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22

Stamsås, Gro Anita, Leiv Sigve Håvarstein, and Daniel Straume. "CHiC, a new tandem affinity tag for the protein purification toolbox." Journal of Microbiological Methods 92, no. 1 (2013): 59–63. http://dx.doi.org/10.1016/j.mimet.2012.11.003.

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23

Guo, Longhua, Wantao Ying, Jiyang Zhang, et al. "Tandem affinity purification and identification of the human TSC1 protein complex." Acta Biochimica et Biophysica Sinica 42, no. 4 (2010): 266–73. http://dx.doi.org/10.1093/abbs/gmq014.

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24

Graumann, Johannes, Leslie A. Dunipace, Jae Hong Seol, et al. "Applicability of Tandem Affinity Purification MudPIT to Pathway Proteomics in Yeast." Molecular & Cellular Proteomics 3, no. 3 (2003): 226–37. http://dx.doi.org/10.1074/mcp.m300099-mcp200.

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25

Tasto, Joseph J., Robert H. Carnahan, W. Hayes McDonald, and Kathleen L. Gould. "Vectors and gene targeting modules for tandem affinity purification inSchizosaccharomyces pombe." Yeast 18, no. 7 (2001): 657–62. http://dx.doi.org/10.1002/yea.713.

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26

Gregan, Juraj, Christian G. Riedel, Mark Petronczki, et al. "Tandem affinity purification of functional TAP-tagged proteins from human cells." Nature Protocols 2, no. 5 (2007): 1145–51. http://dx.doi.org/10.1038/nprot.2007.172.

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27

Schäffer, Ursula, Andreas Schlosser, Kristian M. Müller, et al. "SnAvi – a new tandem tag for high-affinity protein-complex purification." Nucleic Acids Research 38, no. 6 (2010): e91-e91. http://dx.doi.org/10.1093/nar/gkp1178.

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28

Hafrén, Anders, and Kristiina Mäkinen. "Purification of viral genome-linked protein VPg from potato virus A-infected plants reveals several post-translationally modified forms of the protein." Journal of General Virology 89, no. 6 (2008): 1509–18. http://dx.doi.org/10.1099/vir.0.83649-0.

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In order to be able to analyse post-translational modifications and protein interactions of viral genome-linked protein VPg taking place during potato virus A (PVA) infection, an affinity tag-based purification system was developed by inserting a sequence encoding a six-histidine and haemagglutinin (HisHA) tag to the 3′ end of the VPg coding sequence within the infectious cDNA clone of PVA. The engineered virus was fully functional and the HisHA tag-encoding sequence remained stable in the PVA genome throughout the infection process. Purification under denaturing conditions resulted in a prote
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29

Völkel, Pamela, Perrine Le Faou, and Pierre-Olivier Angrand. "Interaction proteomics: characterization of protein complexes using tandem affinity purification–mass spectrometry." Biochemical Society Transactions 38, no. 4 (2010): 883–87. http://dx.doi.org/10.1042/bst0380883.

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Most cellular processes are carried out by a multitude of proteins that assemble into multimeric complexes. Thus a precise understanding of the biological pathways that control cellular events relies on the identification and on the biochemical characterization of the proteins involved in such multimeric assemblies. Advances in MS have made possible the identification of multisubunit protein complexes isolated from cell lysates with high sensitivity and accuracy, whereas the TAP (tandem affinity purification) methodology efficiently isolates native protein complexes from cells for proteomics a
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30

Takebe, Sachiko, William Harold Witola, Bernd Schimanski, Arthur Günzl, and Choukri Ben Mamoun. "Purification of Components of the Translation Elongation Factor Complex of Plasmodium falciparum by Tandem Affinity Purification." Eukaryotic Cell 6, no. 4 (2007): 584–91. http://dx.doi.org/10.1128/ec.00376-06.

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ABSTRACT Plasmodium falciparum is the causative agent of severe human malaria, responsible for over 2 million deaths annually. Of the 5,300 polypeptides predicted to control the parasite life cycle in mosquitoes and humans, 60% are of unknown function. A major challenge of malaria postgenomic biology is to understand how the 5,300 predicted proteins coexist and interact to perform the essential tasks that define the complex life cycle of the parasite. One approach to assign function to these proteins is by identifying their physiological partners. Here we describe the use of tandem affinity pu
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31

Puri, Pawan, Amparo Acker-Palmer, Ryan Stahler, Yijing Chen, Douglas Kline, and Srinivasan Vijayaraghavan. "Identification of testis 14–3-3 binding proteins by tandem affinity purification." Spermatogenesis 1, no. 4 (2011): 354–65. http://dx.doi.org/10.4161/spmg.1.4.18902.

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32

Kyriakakis, Phillip P., Marla Tipping, Louka Abed, and Alexey Veraksa. "Tandem affinity purification in Drosophila: the advantages of the GS-TAP system." Fly 2, no. 4 (2008): 229–35. http://dx.doi.org/10.4161/fly.6669.

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33

Assadi, Maziar, Thomas Schindler, Bernd Muller, John D. Porter, Markus A. Ruegg, and Hanno Langen. "Identification of Proteins Interacting with Dysferlin Using the Tandem Affinity Purification Method." Open Cell Development & Biology Journal 1 (June 17, 2008): 17–23. http://dx.doi.org/10.2174/1874085500801010017.

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34

Borroto Escuela, Dasiel Oscar, Mileydis Perez Alea, Wilber Romero Fernandez, and Daniel Bello Gil. "Vectors and P64k gene targeting for tandem affinity purification in Neisseria meningitidis." Journal of Microbiological Methods 65, no. 1 (2006): 187–93. http://dx.doi.org/10.1016/j.mimet.2005.07.013.

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35

Gully, D., D. Moinier, L. Loiseau, and E. Bouveret. "New partners of acyl carrier protein detected inEscherichia coliby tandem affinity purification." FEBS Letters 548, no. 1-3 (2003): 90–96. http://dx.doi.org/10.1016/s0014-5793(03)00746-4.

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36

Tagwerker, Christian, Karin Flick, Meng Cui, et al. "A Tandem Affinity Tag for Two-step Purification under Fully Denaturing Conditions." Molecular & Cellular Proteomics 5, no. 4 (2006): 737–48. http://dx.doi.org/10.1074/mcp.m500368-mcp200.

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37

Ma, Hanhui, Janel R. McLean, Lucy Fang-I. Chao, et al. "A Highly Efficient Multifunctional Tandem Affinity Purification Approach Applicable to Diverse Organisms." Molecular & Cellular Proteomics 11, no. 8 (2012): 501–11. http://dx.doi.org/10.1074/mcp.o111.016246.

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38

Mayer, Daniel, Sacha Baginsky, and Martin Schwemmle. "Isolation of viral ribonucleoprotein complexes from infected cells by tandem affinity purification." PROTEOMICS 5, no. 17 (2005): 4483–87. http://dx.doi.org/10.1002/pmic.200402095.

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39

Shafey, Dina, Justin G. Boyer, Kunal Bhanot, and Rashmi Kothary. "Identification of Novel Interacting Protein Partners of SMN Using Tandem Affinity Purification." Journal of Proteome Research 9, no. 4 (2010): 1659–69. http://dx.doi.org/10.1021/pr9006987.

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40

Bürckstümmer, Tilmann, Keiryn L. Bennett, Adrijana Preradovic, et al. "An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells." Nature Methods 3, no. 12 (2006): 1013–19. http://dx.doi.org/10.1038/nmeth968.

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41

Rohila, Jai S., Mei Chen, Shuo Chen, et al. "Protein-protein interactions of tandem affinity purification-tagged protein kinases in rice." Plant Journal 46, no. 1 (2006): 1–13. http://dx.doi.org/10.1111/j.1365-313x.2006.02671.x.

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42

Saracco, Scott A., Maria Hansson, Mark Scalf, Joseph M. Walker, Lloyd M. Smith, and Richard D. Vierstra. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Plant Journal 59, no. 2 (2009): 344–58. http://dx.doi.org/10.1111/j.1365-313x.2009.03862.x.

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43

Rubio, Vicente, Yunping Shen, Yusuke Saijo, et al. "An alternative tandem affinity purification strategy applied to Arabidopsis protein complex isolation." Plant Journal 41, no. 5 (2005): 767–78. http://dx.doi.org/10.1111/j.1365-313x.2004.02328.x.

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44

Golebiowski, Filip, Michael H. Tatham, Akihiro Nakamura, and Ronald T. Hay. "High-stringency tandem affinity purification of proteins conjugated to ubiquitin-like moieties." Nature Protocols 5, no. 5 (2010): 873–82. http://dx.doi.org/10.1038/nprot.2010.40.

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45

Cipak, Lubos, Tomas Selicky, Jan Jurcik, et al. "Tandem affinity purification protocol for isolation of protein complexes from Schizosaccharomyces pombe." STAR Protocols 3, no. 1 (2022): 101137. http://dx.doi.org/10.1016/j.xpro.2022.101137.

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46

Xu, Xiaoli, Yuan Song, Yuhua Li, Jianfeng Chang, Hua zhang, and Lizhe An. "The tandem affinity purification method: An efficient system for protein complex purification and protein interaction identification." Protein Expression and Purification 72, no. 2 (2010): 149–56. http://dx.doi.org/10.1016/j.pep.2010.04.009.

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47

Drakas, Robert, Marco Prisco, and Renato Baserga. "A modified tandem affinity purification tag technique for the purification of protein complexes in mammalian cells." PROTEOMICS 5, no. 1 (2005): 132–37. http://dx.doi.org/10.1002/pmic.200400919.

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48

Tsai, Arthur, and Russ P. Carstens. "An optimized protocol for protein purification in cultured mammalian cells using a tandem affinity purification approach." Nature Protocols 1, no. 6 (2006): 2820–27. http://dx.doi.org/10.1038/nprot.2006.371.

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49

Jerlström-Hultqvist, Jon, Britta Stadelmann, Sandra Birkestedt, Ulf Hellman, and Staffan G. Svärd. "Plasmid Vectors for Proteomic Analyses in Giardia: Purification of Virulence Factors and Analysis of the Proteasome." Eukaryotic Cell 11, no. 7 (2012): 864–73. http://dx.doi.org/10.1128/ec.00092-12.

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ABSTRACTIn recent years, proteomics has come of age with the development of efficient tools for purification, identification, and characterization of gene products predicted by genome projects. The intestinal protozoanGiardia intestinaliscan be transfected, but there is only a limited set of vectors available, and most of them are not user friendly. This work delineates the construction of a suite of cassette-based expression vectors for use inGiardia. Expression is provided by the strong constitutive ornithine carbamoyltransferase (OCT) promoter, and tagging is possible in both N- and C-termi
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50

Schimanski, Bernd, Tu N. Nguyen, and Arthur Günzl. "Highly Efficient Tandem Affinity Purification of Trypanosome Protein Complexes Based on a Novel Epitope Combination." Eukaryotic Cell 4, no. 11 (2005): 1942–50. http://dx.doi.org/10.1128/ec.4.11.1942-1950.2005.

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ABSTRACT Tandem affinity purification (TAP) allows for rapid and efficient purification of epitope-tagged protein complexes from crude extracts under native conditions. The method was established in yeast and has been successfully applied to other organisms, including mammals and trypanosomes. However, we found that the original method, which is based on the TAP tag, consisting of a duplicate protein A epitope, a tobacco etch virus protease cleavage site, and the calmodulin-binding peptide (CBP), did not yield enough recovery of transcription factor SNAPc (for small nuclear RNA-activating prot
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