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Journal articles on the topic 'TargetScan'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 February 2022

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1

Shinde, Santosh, and Utpal Bhadra. "MicroRNA Gene Interaction in Amyotrophic Lateral Sclerosis Dataset." Dataset Papers in Science 2014 (June 30, 2014): 1–24. http://dx.doi.org/10.1155/2014/780726.

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All microRNAs (miRNAs) in amyotrophic lateral sclerosis (ALS) study were collected from public databases such as miRBase, mir2Disease, and Human miRNA and Disease Database (HMDD). These miRNA datasets were used for target identification; these sets of miRNAs were expressed in brain specific parts of brain such as midbrain, cerebellum, frontal cortex, and hippocampus. Gene’s information and sequences were collected from NCBI and KEGG databases. All miRNAs were used for target prediction against 35 ALS associated genes. Three programs were used for target identification, namely, miRanda, TargetS

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Tadtayev, S., E. Mazaris, L. Fowler, and G. Boustead. "UP-02.141 TargetScan 3D Mapping Biopsies of the Prostate in Men With Prior Negative Biopsies." Urology 78, no. 3 (2011): S308—S309. http://dx.doi.org/10.1016/j.urology.2011.07.959.

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Mon‐López, Daniel, and Carlos M. Tejero‐González. "Validity and reliability of the TargetScan ISSF Pistol & Rifle application for measuring shooting performance." Scandinavian Journal of Medicine & Science in Sports 29, no. 11 (2019): 1707–12. http://dx.doi.org/10.1111/sms.13515.

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Bollmann, Stephanie, Dengpan Bu, Jiaqi Wang, and Massimo Bionaz. "Unmasking Upstream Gene Expression Regulators with miRNA-corrected mRNA Data." Bioinformatics and Biology Insights 9S4 (January 2015): BBI.S29332. http://dx.doi.org/10.4137/bbi.s29332.

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Expressed micro-RNA (miRNA) affects messenger RNA (mRNA) abundance, hindering the accuracy of upstream regulator analysis. Our objective was to provide an algorithm to correct such bias. Large mRNA and miRNA analyses were performed on RNA extracted from bovine liver and mammary tissue. Using four levels of target scores from TargetScan (all miRNA:mRNA target gene pairs or only the top 25%, 50%, or 75%) Using four levels of target scores from TargetScan (all miRNA:mRNA target gene pairs or only the top 25%, 50%, or 75%) and four levels of the magnitude of miRNA effect (ME) on mRNA expression (3

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Lv, Teng, Kejuan Song, Lili Zhang, et al. "miRNA-34a decreases ovarian cancer cell proliferation and chemoresistance by targeting HDAC1." Biochemistry and Cell Biology 96, no. 5 (2018): 663–71. http://dx.doi.org/10.1139/bcb-2018-0031.

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This study aimed to explore the roles of miRNA-34a (miR-34a) in ovarian cancer (OC) cells and uncover possible mechanisms. The proliferation of OC cells was measured with an MTT assay and soft agar colony formation assay. TargetScan analysis, real-time PCR, and a luciferase reporter assay were used to demonstrate the downstream target of miR-34a in OC cells. HDAC1 expression levels were detected by immunoblot analysis. miR-34a inhibited the proliferation of SKOV3 and OVCA433 cells and enhanced cisplatin sensitivity in cisplatin-resistant SKOV3cp cells. The results of TargetScan analysis, real-

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Shi, Yuxia, Fan Yang, Shuqing Wei, and Gang Xu. "Identification of Key Genes Affecting Results of Hyperthermia in Osteosarcoma Based on Integrative ChIP-Seq/TargetScan Analysis." Medical Science Monitor 23 (April 28, 2017): 2042–48. http://dx.doi.org/10.12659/msm.901191.

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Mustafa, Rima, Mohsen Ghanbari, Marina Evangelou, and Abbas Dehghan. "An Enrichment Analysis for Cardiometabolic Traits Suggests Non-Random Assignment of Genes to microRNAs." International Journal of Molecular Sciences 19, no. 11 (2018): 3666. http://dx.doi.org/10.3390/ijms19113666.

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MicroRNAs (miRNAs) regulate the expression of the majority of genes. However, it is not known whether they regulate genes in random or are organized according to their function. To this end, we chose cardiometabolic disorders as an example and investigated whether genes associated with cardiometabolic disorders are regulated by a random set of miRNAs or a limited number of them. Single-nucleotide polymorphisms (SNPs) reaching genome-wide level significance were retrieved from most recent genome-wide association studies on cardiometabolic traits, which were cross-referenced with Ensembl to iden

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Woo, Chin Cheng, Wenting Liu, Xiao Yun Lin, et al. "The Interaction between 30b-5p miRNA and MBNL1 mRNA is Involved in Vascular Smooth Muscle Cell Differentiation in Patients with Coronary Atherosclerosis." International Journal of Molecular Sciences 21, no. 1 (2019): 11. http://dx.doi.org/10.3390/ijms21010011.

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Vascular smooth muscle cells (VSMCs) in the arterial wall have diverse functions. In pathological states, the interplay between transcripts and microRNAs (miRNAs) leads to phenotypic changes. Understanding the regulatory role of miRNAs and their target genes may reveal how VSMCs modulate the pathogenesis of coronary artery disease. Laser capture microdissection was performed on aortic wall tissues obtained from coronary artery bypass graft patients with and without recent acute myocardial infarction (MI). The mSMRT-qPCR miRNA assay platform (MiRXES, Singapore) was used to profile miRNA. The mi

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Zhang, Jun, Suli Lu, Ting Ding, Haijia Zhao, and Dongxing Tang. "MiR-384 is associated with renal damage in lupus nephritis via regulation of TET3 expression." Tropical Journal of Pharmaceutical Research 19, no. 12 (2021): 2571–76. http://dx.doi.org/10.4314/tjpr.v19i12.13.

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Purpose: To investigate the correlations between miR-384 expression and renal damage in lupus nephritis (LN).Methods: Lupus nephritis and normal tissues were collected during surgery. The relative miR-384 expression was evaluated by extracting RNA and performing quantitative real time PCR (qRT-PCR) assays. Expression of ten-eleven translocation (TET3) mRNA and protein were measured by qRT-PCR and western blotting, respectively. The 24-h urine protein, serum complement C3, and serum creatinine were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits. TargetScan and lucife

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Wang, Yibiao, and Min Xu. "miR-380-5p facilitates NRF2 and attenuates cerebral ischemia/reperfusion injury-induced neuronal cell death by directly targeting BACH1." Translational Neuroscience 12, no. 1 (2021): 210–17. http://dx.doi.org/10.1515/tnsci-2020-0172.

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Abstract Background This study aimed to explore the role of miR-380-5p in cerebral ischemia/reperfusion (CIR) injury-induced neuronal cell death and the potential signaling pathway involved. Methodology Human neuroblastoma cell line SH-SY5Y cells were used in this study. Oxygen and glucose deprivation/reperfusion (OGD/R) model was used to mimic ischemia/reperfusion injury. CCK-8 assay and flow cytometry were used to examine cell survival. Quantitative real time PCR (RT-qPCR) assay and Western blotting were used to measure the change of RNA and protein expression, respectively. TargetScan and L

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Zhang, Zhongjie, Yan Xu, Songyuan Chi, and Longji Cui. "MicroRNA-582-5p Reduces Propofol-induced Apoptosis in Developing Neurons by Targeting ROCK1." Current Neurovascular Research 17, no. 2 (2020): 140–46. http://dx.doi.org/10.2174/1567202617666200207124817.

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Background: Propofol is an intravenous drug commonly used in anesthesia procedures and intensive care in children. However, it also has neurotoxic effects on children. MicroRNA plays an important role in neurological diseases and neurotoxicity. Methods: In this study, primary rat hippocampal neurons were used to investigate the role of miR- 582-5p in propofol-induced neurotoxicity. Cell viability was monitored by 3-(4,5-dimethylthiazolyl)- 2,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, while the expression of proteins was monitored by real-time quantitation polymerase c

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Hu, Yi, Yan Ma, Guifang Luo, Wenyan Liao, Shufen Zhang, and Genlin Li. "Effect of MiR-375 Regulates YAP1 on the Invasion, Apoptosis, and Epithelial-Mesenchymal Transition of Cervical Cancer HeLa Cells." Evidence-Based Complementary and Alternative Medicine 2021 (September 1, 2021): 1–8. http://dx.doi.org/10.1155/2021/3088723.

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Yes-associated protein 1 (YAP1) is an important signaling pathway activator molecule. Studies have shown that it is involved in the occurrence of malignant tumors. This study identified a microRNA (miR/miRNA) targeting the 3′ untranslated region (3″ utr) of the YAP1 gene and evaluated its biological impact on human cervical cancer cells and related molecular mechanisms. qPCR and western blotting were used to detect the levels of miR-375 and YAP1 in HeLa cells. TargetScan software was used to identify the binding sites of YAP1 and miR-375. The MTT method was used to determine the viability of H

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Chen, Xiao, Zhaosheng Ding, Tong Li, Wei Jiang, Jiawei Zhang, and Xuejun Deng. "MicroR-26b Targets High Mobility Group, AT-hook 2 to Ameliorate Myocardial Infarction-induced Fibrosis by Suppression of Cardiac Fibroblasts Activation." Current Neurovascular Research 17, no. 2 (2020): 204–13. http://dx.doi.org/10.2174/1567202617666200506101258.

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Background: Myocardial Fibrosis (MF) is an important physiological change after myocardial infarction (MI). MicroRNA-26b (MiR-26b) has a certain inhibitory effect on pulmonary fibrosis. However, the role of miR-26b in MI-induced MF rats and underlying molecular mechanisms remain unknown. Methods: Forty male Sprague Dawley (SD) rats weighing 200-250 g were divided into four groups (n=10): Sham group, MF group, MF + negative control (NC) agomir group and MF + miR-26b agomir group. Cardiac fibroblasts were isolated from cardiac tissue. Fibrosis levels were detected by MASSON staining, while the e

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Liu, Sulai, Honglian Zou, Yonggang Wang, et al. "miR-155-5p is Negatively Associated with Acute Pancreatitis and Inversely Regulates Pancreatic Acinar Cell Progression by Targeting Rela and Traf3." Cellular Physiology and Biochemistry 51, no. 4 (2018): 1584–99. http://dx.doi.org/10.1159/000495648.

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Background/Aims: Acute pancreatitis contributes to high mortality in pancreatitis patients, and miRNAs play a vital role in the development of acute pancreatitis (AP), however, its precise biological role remains largely elusive. Methods: To clarify the potential mechanisms of miRNAs in AP, we built mouse models of mild acute pancreatitis (MAP) and moderate/ severe acute pancreatitis (SAP). MiRNA microarray analysis and Real-time quantitative PCR (qRT-PCR) were used to analyze the expression of miRNA in MAP/SAP. TargetScan software, dual-luciferase gene reporter assays and Western blotting wer

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An, Ning, and Bo Zheng. "MiR-203a-3p Inhibits Pancreatic Cancer Cell Proliferation, EMT, and Apoptosis by Regulating SLUG." Technology in Cancer Research & Treatment 19 (January 1, 2020): 153303381989872. http://dx.doi.org/10.1177/1533033819898729.

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Objective: The aim of the present research is to study the roles of miR-203a-3p on cell proliferation, migration, invasion, and epithelial–mesenchymal transition in pancreatic cancer. Methods: Transcription profiles were acquired from Gene Expression Omnibus database, which was used to screen out the differentially expressed microRNAs and messenger RNAs in pancreatic cancer. Pancreatic cancer tissues were used to verify the bioinformatics results by quantitative real-time polymerase chain reaction. The relationship between miR-203a-3p and SLUG was examined by TargetScan software, dual-lucifera

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Taneja, Samir S., Guilherme Godoy, Adam S. Kibel, David F. Penson, and John T. Wei. "PROSTATE CANCER DETECTION USING A NOVEL COMPUTERIZED THREE-DIMENSIONAL PROSTATE BIOPSY TEMPLATE (TARGETSCAN™): RESULTS OF A MULTICENTER PROSPECTIVE DATA REGISTRY." Journal of Urology 181, no. 4 (2009): 712. http://dx.doi.org/10.1016/s0022-5347(09)61989-3.

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Shen, Julian, Wei Wei, Xialei Wang, et al. "Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK1/2 and miR-17∼92a Cluster Activation." Evidence-Based Complementary and Alternative Medicine 2020 (August 24, 2020): 1–12. http://dx.doi.org/10.1155/2020/7275246.

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Context: Alismatis rhizome decoction (AD) exhibits antiatherosclerotic activities. The activity of AD against vascular smooth muscle cell (VSMC) proliferation remains unclear. Objective. The mechanisms and effects of AD on oxidized low-density lipoprotein (ox-LDL)-induced VSMC proliferation were explored. Materials and methods. The male SD rats were fed with AD (2.56 g/mL) or 0.9% NaCl by oral gavage 4 mL twice daily for 7 d. Then, AD-containing serum (ADcs) was collected. MTS assay was applied to measure the VSMC viability. The proliferation of VSMCs was detected by 5-bromodeoxyuridine (BrdU)

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Alphonse, P., N. Hood, S. Lyn, J. Foote, and J. Bennett. "MP-4.09: TargetScan ®: A Novel Approach for Outpatient Prostate Biopsy with the Potential for Use as an Aid to Focal Prostate Therapy." Urology 72, no. 5 (2008): S86—S87. http://dx.doi.org/10.1016/j.urology.2008.08.251.

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Xu, Jiahong, Yang Liu, Yuan Xie, Cuimei Zhao, and Hongbao Wang. "Bioinformatics Analysis Reveals MicroRNAs Regulating Biological Pathways in Exercise-Induced Cardiac Physiological Hypertrophy." BioMed Research International 2017 (2017): 1–6. http://dx.doi.org/10.1155/2017/2850659.

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Exercise-induced physiological cardiac hypertrophy is generally considered to be a type of adaptive change after exercise training and is beneficial for cardiovascular diseases. This study aims at investigating exercise-regulated microRNAs (miRNAs) and their potential biological pathways. Here, we collected 23 miRNAs from 8 published studies. MirPath v.3 from the DIANA tools website was used to execute the analysis, and TargetScan was used to predict the target genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses were performed to identify potential pathways an

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Li, Sierra Mi, Xiwei Wu, Paul Henry Frankel, et al. "Correlation between miRNA (miR) and gene expression profiles (GEP) and response to neoadjuvant chemotherapy (NT) in patients with locally advanced and inflammatory breast cancer (BC)." Journal of Clinical Oncology 30, no. 15_suppl (2012): 10545. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.10545.

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10545 Background: GEP may predict for pathologic complete response (pCR). Correlation between miRs, GEP, and pCR may reveal novel targets. Methods: Patients (pts) with HER2- BC were randomized to receive docetaxel, doxorubicin, cyclophosphamide (TAC, arm A) or A and C given every 2 weeks x 4, followed by carboplatin and nab-paclitaxel (arm B). Pts with HER2+ BC received NT per arm B, with trastuzumab added (arm C). Core biopsies were snap-frozen prior to NT, and RNA extracted for gene array analysis (Agilent 44K microarray) and deep sequence miRNA analysis (Solexa/Illumina platform). HER2 test

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Suraokar, M., A. Corvalan, C. Chow, et al. "Integrating microRNA and mRNA expression profiling using a novel algorithm identified a small set of unique genes upregulated in malignant pleural mesothelioma (MPM)." Journal of Clinical Oncology 27, no. 15_suppl (2009): e22111-e22111. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.e22111.

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e22111 Background: We employed a global profiling strategy using miRNA microarrays in MPM cell lines and archival tumor tissue. Methods: We isolated total RNA from 4 MPM cell lines, 2 control cell lines, and 16 tissue specimens from patients with resected MPM (n=8) and normal counterpart (n=8) patients as controls. Total RNA was labeled with Cyanine 3, then hybridized with Agilent human miRNA microarray v1 slides. Results: Preliminary miRNA profiles show up-regulation of 44 versus down-regulation of 29 miRNA's in MSTO-211H cancer cells compared to HCT-4012 (pleural telomerase-transformed contr

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Qiu, Zhenghua, Lingjing Tu, Xiongwei Hu, et al. "A Preliminary Study of miR-144 Inhibiting the Stemness of Colon Cancer Stem Cells by Targeting Krüppel-Like Factor 4." Journal of Biomedical Nanotechnology 16, no. 7 (2020): 1102–9. http://dx.doi.org/10.1166/jbn.2020.2952.

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Colon cancer is a prevalent clinical malignant tumor of the digestive system. The current study aims to explore the miR-144 expression in colorectal cancer (CRC) cell lines and CRC stem cells (CSCs) and to explore its effect on the stemness of CSCs and the targeted regulation of Krüppel-like factor 4 (KLF4). Use qRT-PCR to detect the expression level of miR-144 in CRC cells SW480, HCT116, and H129 and the healthy colon cell NCM460. The CSCs that were used were cultured in HCT116 cells. Use western blot to explore the expressions of Nanog, SOX2, and OCT4 stemness marker protein. After it was tr

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Zhang, Jianjun, Jingjing Sha, Yan Zhou, et al. "Bufalin Inhibits Proliferation and Induces Apoptosis in Osteosarcoma Cells by Downregulating MicroRNA-221." Evidence-Based Complementary and Alternative Medicine 2016 (2016): 1–10. http://dx.doi.org/10.1155/2016/7319464.

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Bufalin, a major component of the Chinese medicine ChanSu, which is prepared from the skin and parotid venom glands of toads, has shown cytotoxicity in several malignant tumors. Here, we reported that bufalin inhibited proliferation and induced mitochondria-dependent apoptosis in U-2OS and Saos-2 osteosarcoma cells with intracellular reactive oxygen species (ROS) production. By microRNA (miR) array analysis and quantitative reverse transcription polymerase chain reaction, we found that miR-221 was downregulated after treatment with bufalin. In accordance with TargetScan prediction and lucifera

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Zhou, Lili, Lingzhi Li, Yan Chen, et al. "miR-190a-3p Promotes Proliferation and Migration in Glioma Cells via YOD1." Computational and Mathematical Methods in Medicine 2021 (September 4, 2021): 1–12. http://dx.doi.org/10.1155/2021/3957738.

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Introduction. To investigate the function of miR-190a-3p on the proliferation and migration of glioma. Methods. Twenty glioma samples and 6 normal brain tissue samples were collected. Normal human glial cell line HEB and glioma cell lines were used for the experiments. We then used TargetScan to predict the target genes of miR-190a-3p. Dual-luciferase reporter assay was also used to validate. Results. Combined with dual-luciferase reporter experiment, we finally verified that YOD1 was the aim, and it was low-expressed in glioma. Besides, a series of mechanism experiments then proved that miR-1

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Jia, Hongshuai, and Chunsheng Hao. "Exploring dysregulated miRNAs in cryptorchidism: a systematic review." Journal of International Medical Research 49, no. 3 (2021): 030006052199995. http://dx.doi.org/10.1177/0300060521999950.

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Objective To identify dysregulated miRNAs in testicular tissues from animal models and patients with cryptorchidism. Methods Databases were systematically searched for studies published before 10 May 2020 that had investigated miRNAs in cryptorchidism. Predicted targets of the identified miRNA biomarkers were obtained by searching TargetScan and Starbase. Gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analyses were subsequently conducted. Results Five publications met the eligibility criteria for the review. 21 differentially expressed miRNAs were the

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Wang, Jianmin, Dongliang Zhou, Hongwei Qin, Ying Xu, Ying Guan, and Weidong Zang. "Screening of Key Genes Associated with Ischemic Stroke via Microarray Data." Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques 40, no. 6 (2013): 864–69. http://dx.doi.org/10.1017/s0317167100016036.

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Objective:To promote understandings about the pathogenesis of ischemic stroke (IS) through mining key genes, functions and pathways with microarray technology.Methods:Differentially expressed genes (DEGs) in blood between patients with IS and healthy people were screened out through comparing microarray data obtained from Gene Expression Omnibus. Overrepresented functions in DEGs were revealed by Gene Ontology (GO) enrichment analysis. Interaction network was constructed for the top 24 DEGs with information from Human Protein Reference Database (HPRD). Relevant microRNAs (miRNAs) were retrieve

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Zhu, Yu, Chengmao Zhou, and Qixiong He. "High miR-139-3p expression predicts a better prognosis for hepatocellular carcinoma: a pooled analysis." Journal of International Medical Research 47, no. 1 (2018): 383–90. http://dx.doi.org/10.1177/0300060518802727.

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Objective To observe the expression and clinical significance of micro RNA (miR)-139-3p in liver cancer tissues, and to explore its relationship with miR-139-3p target genes related to the prognosis of hepatocellular carcinoma (HCC). Methods A total of 362 patients with HCC were included in the study. Liver hepatocellular carcinoma data were obtained directly from The Cancer Genome Atlas data portal .The bioinformatics analysis tool TargetScan was applied to predict miR-139-3p target genes. Results Survival time was significantly higher in patients with high miR-139-3p expression, compared wit

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Wang, Hui, Jing Shi, Beibei Li, Qiulian Zhou, Xiangqing Kong, and Yihua Bei. "MicroRNA Expression Signature in Human Calcific Aortic Valve Disease." BioMed Research International 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/4820275.

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Altered microRNA (miRNA, miR) expression has been related to many disease processes; however, the miRNA expression signature in calcific aortic valve disease (CAVD) is unclear. In this study, microarrays were used to determine the miRNA expression signature of tissue samples from healthy individuals (n=4) and patients with CAVD (n=4). TargetScan, PITA, and microRNAorg 3-way databases were used to predict the potential target genes. DIANA-miRPath was used to incorporate the aberrant miRNAs into gene pathways. miRNA microarrays identified 92 differentially expressed miRNAs in CAVD tissues. The p

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Hu, Guangyao, and Dianxiu Wu. "miR-4262 Targets Anti-Apoptotic Gene B-Cell Lymphoma-2 to Induce Cell Apoptosis and Inhibit Cell Proliferation in Oral Squamous Cell Carcinoma." Journal of Biomaterials and Tissue Engineering 10, no. 6 (2020): 804–11. http://dx.doi.org/10.1166/jbt.2020.2336.

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Oral squamous cell carcinoma (OSCC), a frequently happened cancer, is still an important threaten to human with unsatisfactory prognosis. Increasing evidence indicated that abnormal miRNA expressions were related to the development of OSCC. microRNA (miR)-4262 has been considered to be a cancer suppressor in various tumors, however its exact role in OSCC remains to be clarified. During the current research, we proposed to probe the biological activity and fundamental mechanism of miR-4262 in OSCC. The expression levels of miR-4262 in SCC9 and HOK cells were analyzed using quantitative real tim

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Santosh P., Shinde, Neelima Arora, Pranjal Sarma, Manika Pal-Bhadra, and Utpal Bhadra. "Interaction Map and Selection of microRNA Targets in Parkinson's Disease-Related Genes." Journal of Biomedicine and Biotechnology 2009 (2009): 1–11. http://dx.doi.org/10.1155/2009/363145.

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Parkinson's disease (PD) is a complex multigenic neurodisorder frequently occurring in elderly persons. To investigate noncoding tiny microRNA mediated gene regulation, miRanda (version 1.0b) was used to predict human miRNA target sites on selected 29 genes related to PD. To verify output generated from miRanda, a similar analysis was performed only for microRNA target sites in3′UTR using TargetScan (version 5.1). Data extracted by miRanda elucidates the mode of microRNA action based on the location of target sites in the Parkinson genes. Sites prone to action of multiple miRNAs were identifie

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Zhu, Zheng, Yuhua Qi, Huan Fan, Lunbiao Cui, and Zhiyang Shi. "Systematic Identification and Bioinformatic Analysis of MicroRNAs in Response to Infections of Coxsackievirus A16 and Enterovirus 71." BioMed Research International 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/4302470.

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Hand, foot, and mouth disease (HFMD), mainly caused by coxsackievirus A16 (CVA16) and enterovirus 71 (EV71) infections, remains a serious public health issue with thousands of newly diagnostic cases each year since 2008 in China. The mechanisms underlying viral infection, however, are elusive to date. In the present study, we systematically investigated the host cellular microRNA (miRNA) expression patterns in response to CVA16 and EV71 infections. Through microarray examination, 27 miRNAs (15 upregulated and 12 downregulated) were found to be coassociated with the replication process of two v

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Li, Enfang, Ke Han, and Xuan Zhou. "microRNA-27a-3p down-regulation inhibits malignant biological behaviors of ovarian cancer by targeting BTG1." Open Medicine 14, no. 1 (2019): 577–85. http://dx.doi.org/10.1515/med-2019-0065.

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AbstractOvarian cancer is the most deadly malignant tumor. MicroRNA-27a-3p (miR-27a-3p) was a tumor oncogene in various cancers. However, the role and mechanism of miR-27a-3p in ovarian cancer are still unknown. In this study, we found that miR-27a-3p over-expression could significantly promote the viability of SK-OV-3 cells, enhance cell migration and invasion, and reduce cell apoptosis. Besides, results from western blot assay showed that miR-27a-3p over-expression could increase Bcl-2 protein expression and decrease Bax protein expression. Furthermore, TargetScan and the dual luciferase rep

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Lin, Jie, Jun Jiang, Ruifang Zhou, Xiaojie Li, and Jun Ye. "MicroRNA-451b participates in coronary heart disease by targeting VEGFA." Open Medicine 15, no. 1 (2019): 1–7. http://dx.doi.org/10.1515/med-2020-0001.

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AbstractCoronary artery disease (CAD) is one of the main causes of hospitalization worldwide and has high morbidity. MicroRNAs (miRNAs) play an important role in the pathogenesis of cardiovascular diseases. miR-451 is a special miRNA that is involved in many cancers’ development. At present, there is no research about miR-451 in coronary heart disease. In this study, we aimed to identify the action mechanism of miR-451 in coronary heart disease and human umbilical vein endothelial cells (HUVECs). In this study, we found that miR-451 is up-regulated in the peripheral blood of patients with coro

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Kurita, Hisaka, Saori Yabe, Tomoyuki Ueda, Masatoshi Inden, Akiyoshi Kakita, and Isao Hozumi. "MicroRNA-5572 Is a Novel MicroRNA-Regulating SLC30A3 in Sporadic Amyotrophic Lateral Sclerosis." International Journal of Molecular Sciences 21, no. 12 (2020): 4482. http://dx.doi.org/10.3390/ijms21124482.

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Amyotrophic lateral sclerosis (ALS) is a progressive degenerative disease caused by the loss of motor neurons. Although the pathogenesis of sporadic ALS (sALS) remains unclear, it has recently been suggested that disorders of microRNA (miRNA) may be involved in neurodegenerative conditions. The purpose of this study was to investigate miRNA levels in sALS and the target genes of miRNA. Microarray and real-time RT-PCR analyses revealed significantly-decreased levels of miR-139-5p and significantly increased levels of miR-5572 in the spinal cords of sALS patients compared with those in controls.

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Xu, Tonglei, Fangliang Xie, Dazhou Xu, et al. "MiR-200b Suppresses Gastric Cancer Cell Migration and Invasion by Inhibiting NRG1 through ERBB2/ERBB3 Signaling." Journal of Oncology 2021 (September 7, 2021): 1–10. http://dx.doi.org/10.1155/2021/4470778.

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Purpose. Accumulating evidence indicates that miRNAs (miRs) play crucial roles in the modulation of tumors development. However, the accurately mechanisms have not been entirely clarified. In this study, we aimed to explore the role of miR-200b in the development of gastric cancer (GC). Methods. Western blot and RT-PCR were applied to detect epithelial-mesenchymal transition (EMT) marker expression and mRNA expression. Transwell assay was used for measuring the metastasis and invasiveness of GC cells. TargetScan system, luciferase reporter assay, and rescue experiments were applied for validat

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Hua, Zhaozhao, Dana Li, Anqin Wu, Ting Cao, and Shi Luo. "miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus." Open Medicine 16, no. 1 (2021): 464–71. http://dx.doi.org/10.1515/med-2021-0247.

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Abstract Gestational diabetes mellitus (GDM) is a metabolic dysregulation closely related to both obesity and type 2 diabetes; however, the molecular mechanism underlying GDM is still unclear. The purpose of this study was to investigate the effects of microRNA-377 (miR-377-3p) and fibronectin type III domain containing 5 (FNDC5) in regulating the cell growth of trophoblasts under high glucose (HG) conditions during the development of GDM. Serum miR-377-3p was upregulated and positively correlated with fasting blood glucose level in GDM patients. miR-377-3p downregulation increased the cell vi

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Roccaro, Roccaro M., Antonio Sacco, Abdel Kareem Azab, et al. "MicroRNA Changes Occur in Multiple Myeloma Cells in the Context of Bone Marrow Milieu." Blood 114, no. 22 (2009): 1785. http://dx.doi.org/10.1182/blood.v114.22.1785.1785.

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Abstract Abstract 1785 Poster Board I-811 Background We and Others have previously demonstrated that primary multiple myeloma (MM) cells are characterized by a specific microRNA (miRNA) signature compared to the related normal plasmacell counterpart; and that miRNAs play a crucial role in regulating MM pathogenesis. Nevertheless, miRNA changes that occur in MM cells in the context of the bone marrow microenvironment have not been previously examined. Therefore, characterization of miRNA profiling of MM cells in conjunction with bone marrow stromal cells (BMSCs) is important to better understan

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Chen, Xuewu, and Hongguang Xu. "LncRNA SNHG15 regulates osteosarcoma progression in vitro and in vivo via sponging miR-346 and regulating TRAF4 expression." Open Life Sciences 15, no. 1 (2020): 423–36. http://dx.doi.org/10.1515/biol-2020-0039.

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AbstractOsteosarcoma (OS) is a common primary malignant bone tumor around the world. It has been reported that long noncoding RNAs (lncRNAs) take part in diverse pathological processes of OS; however, the mechanism remains unknown. This study aimed to uncover the profile of lncRNA small nucleolar RNA host gene 15 (SNHG15), its biological function, and its potential involvement in the mechanism of OS progression in vitro and in vivo. The expression of SNHG15 and TRAF4 was promoted in OS tissues opposite for that of miR-346. The silencing of SNHG15 limited the proliferation, invasion, and enhanc

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Zhao, Yong, Hao Wang, Ming Lu, et al. "Pancreatic Acinar Cells Employ miRNAs as Mediators of Intercellular Communication to Participate in the Regulation of Pancreatitis-Associated Macrophage Activation." Mediators of Inflammation 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/6340457.

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Macrophage activation plays an important role in the inflammatory response in acute pancreatitis. In the present study, the activation of AR42J pancreatic acinar cells was induced by taurolithocholate treatment. The results showed that the culture medium from the activated AR42J cells significantly enhanced NFκB activation in the macrophages compared to that without taurolithocholate treatment. Additionally, the precipitates obtained from ultracentrifugation of the culture media that were rich in exosomes were markedly more potent in activating macrophages compared with the supernatant fractio

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Ji, Wen-Feng, Jia-Xin Chen, Shu He, et al. "Characteristics of circular RNAs expression of peripheral blood mononuclear cells in humans with coronary artery disease." Physiological Genomics 53, no. 8 (2021): 349–57. http://dx.doi.org/10.1152/physiolgenomics.00020.2021.

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Circular RNAs (circRNAs) function as promising biomarkers and therapeutic targets for coronary artery disease due to their high stability, covalently closed structure, and potential gene regulation. We aimed to identify the expression profile and role of circular RNAs (circRNAs) in coronary artery disease (CAD). We performed RNA sequence analysis of circRNAs in peripheral blood mononuclear cells of five patients with CAD and five controls. Bioinformatics analyses were adopted to explore biological functions of differentially expressed circRNAs. The miRanda and TargetScan tools were used to pre

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Nagaraj, Siranjeevi, Andrew Want, Katarzyna Laskowska-Kaszub, et al. "Candidate Alzheimer’s Disease Biomarker miR-483-5p Lowers TAU Phosphorylation by Direct ERK1/2 Repression." International Journal of Molecular Sciences 22, no. 7 (2021): 3653. http://dx.doi.org/10.3390/ijms22073653.

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MicroRNAs have been demonstrated as key regulators of gene expression in the etiology of a range of diseases including Alzheimer’s disease (AD). Recently, we identified miR-483-5p as the most upregulated miRNA amongst a panel of miRNAs in blood plasma specific to prodromal, early-stage Alzheimer’s disease patients. Here, we investigated the functional role of miR-483-5p in AD pathology. Using TargetScan and miRTarBase, we identified the microtubule-associated protein MAPT, often referred to as TAU, and the extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), known to phosphorylate T

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Hai, Xiaoyu, Guozhong Zhao, Zhaolong Li, Junli Wu, Xiangzhao Xu, and Yaowen Yang. "Effects of microRNA-103 on the Proliferation and Apoptosis of Pancreatic Cancer Cells via Targeting Phosphatase and Tensin Homolog Deleted on Chromosome Ten (PTEN) and Activating Phosphoinositide 3-Kinase/A Serine/Threonine Kinase (PI3K/Akt) Signaling Pathway." Journal of Biomaterials and Tissue Engineering 11, no. 4 (2021): 690–96. http://dx.doi.org/10.1166/jbt.2021.2583.

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Objective: To investigate whether micro ribonucleic acid (miR)-103 affects pancreatic cancer (PaCa) cells via PTEN-activated PI3K/Akt signaling pathway. Methods: Differences in miR-103 expression in 35 pairs of PaCa tissues and cell lines (SW1990 and PATU8988S) were detected by RT-qPCR. miR-103 inhibitor was transfected into PaCa PATU8988S cell followed by analysis of proliferation and apoptosis of PaCa cells by MTT assay and flow cytometry, respectively. Results: MiR-103 exhibited a significantly high expression in PaCa tissues and cell lines (p < 0.05). Besides, the exogenous inhibition o

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Li, Peng, Yi Chen, Conslata Awino Juma, et al. "Differential Inhibition of Target Gene Expression by Human microRNAs." Cells 8, no. 8 (2019): 791. http://dx.doi.org/10.3390/cells8080791.

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microRNAs (miRNAs) exert their functions by repressing the expression of their target genes, but most miRNA target genes are unknown, and the degree to which a miRNA differentially inhibits the expression of its targets is underappreciated. We selected human miR-1, miR-122, and miR-124 as representatives to investigate the reliability of miRNA target predictions and examine how miRNAs suppress their targets. We constructed miRNA target gene reporter libraries based on prediction programs TargetScan, miRanda, and PicTar, and performed large-scale reporter assays to directly evaluate whether and

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Jiang, Shuxia, Xi Fang, Mingni Liu, Yingdong Ni, Wenqiang Ma, and Ruqian Zhao. "MiR-20b Down-Regulates Intestinal Ferroportin Expression In Vitro and In Vivo." Cells 8, no. 10 (2019): 1135. http://dx.doi.org/10.3390/cells8101135.

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Ferroportin (FPN) is the only known cellular iron exporter in mammalian. However, post-transcriptional regulation of intestinal FPN has not yet been completely understood. In this study, bioinformatics algorithms (TargetScan, PicTar, PITA, and miRanda) were applied to predict, screen and obtain microRNA-17 family members (miR-17, miR-20a, miR-20b, and miR-106a) targeting FPN, ‘seed sequence’ and responding binding sites on the 3′untranslated region (3′UTR) region of FPN. Dual-luciferase reporter assays revealed miRNA-17 family members’ mimics decreased the luciferase activity, whereas their in

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Corsello, Tiziana, Andrzej S. Kudlicki, Roberto P. Garofalo, and Antonella Casola. "Cigarette Smoke Condensate Exposure Changes RNA Content of Extracellular Vesicles Released from Small Airway Epithelial Cells." Cells 8, no. 12 (2019): 1652. http://dx.doi.org/10.3390/cells8121652.

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Exposure to environmental tobacco smoke (ETS) is a known risk factor for the development of chronic lung diseases, cancer, and the exacerbation of viral infections. Extracellular vesicles (EVs) have been identified as novel mediators of cell–cell communication through the release of biological content. Few studies have investigated the composition/function of EVs derived from human airway epithelial cells (AECs) exposed to cigarette smoke condensate (CSC), as surrogates for ETS. Using novel high-throughput technologies, we identified a diverse range of small noncoding RNAs (sncRNAs), including

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Fazio, Sofia, Gabriele Berti, Francesco Russo, et al. "The miR-28-5p Targetome Discovery Identified SREBF2 as One of the Mediators of the miR-28-5p Tumor Suppressor Activity in Prostate Cancer Cells." Cells 9, no. 2 (2020): 354. http://dx.doi.org/10.3390/cells9020354.

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miR-28-5p is downregulated in some tumor tissues in which it has been demonstrated to have tumor suppressor (TS) activity. Here, we demonstrate that miR-28-5p acts as a TS in prostate cancer (PCa) cells affecting cell proliferation/survival, as well as migration and invasion. Using the miRNA pull out assay and next generation sequencing, we collected the complete repertoire of miR-28-5p targets, obtaining a data set (miR-28-5p targetome) of 191 mRNAs. Filtering the targetome with TargetScan 7, PITA and RNA22, we found that 61% of the transcripts had miR-28-5p binding sites. To assign a functio

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Sheu, Chau-Chyun, Wei-An Chang, Ming-Ju Tsai, Ssu-Hui Liao, Inn-Wen Chong, and Po-Lin Kuo. "Gene Expression Changes Associated with Nintedanib Treatment in Idiopathic Pulmonary Fibrosis Fibroblasts: A Next-Generation Sequencing and Bioinformatics Study." Journal of Clinical Medicine 8, no. 3 (2019): 308. http://dx.doi.org/10.3390/jcm8030308.

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Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and fatal interstitial lung disease. Therapeutic options for IPF remain limited. Nintedanib, a tyrosine kinase inhibitor approved for IPF treatment, is known to inhibit fibroblasts proliferation, migration and transformation to myofibroblasts. However, how nintedanib changes gene regulations in IPF has never been systematically investigated. We conducted a next-generation sequencing and bioinformatics study to evaluate the changes of mRNA and miRNA profiles in IPF fibroblasts treated with 2 µM and 4 µM nintedanib, compared to those

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Zhang, Li, Zhang-Qing Wu, Yu-Juan Wang, Meng Wang, and Wu-Cai Yang. "MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells." Animals 10, no. 9 (2020): 1453. http://dx.doi.org/10.3390/ani10091453.

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Milk fat is the main nutritional component of milk and is also an important indicator for evaluating milk quality. Substantial evidence has implicated miRNAs in the synthesis of milk fat. miR-143 is one of the miRNAs closely related to lipid metabolism. Herein, miR-143 upregulation remarkably promoted the production of lipid droplets and increased the level of intracellular triglyceride (TAG). Meanwhile, miR-143 suppression overtly repressed TAG synthesis and lipid droplet accumulation in bovine mammary epithelial cells (BMECs). At the same time, miR-143 significantly upregulated the genes ass

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Wang, Shunmin, Jingchuan Sun, Haisong Yang, et al. "Profiling and bioinformatics analysis of differentially expressed circular RNAs in human intervertebral disc degeneration." Acta Biochimica et Biophysica Sinica 51, no. 6 (2019): 571–79. http://dx.doi.org/10.1093/abbs/gmz036.

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AbstractThe functional changes of nucleus pulposus (NP) cells are considered to be the initiating factors of intervertebral disc degeneration (IDD), and the differentially expressed circRNAs in NP cells may play an important role in the process of IDD. To identify circular RNAs (circRNAs) associated with human IDD, we isolated the NP cells from human degenerated and non-degenerated intervertebral disc and identified NP cells by microscopy and cell proliferation. CircRNA microarray expression profiles were obtained from NP cells of degenerated and non-degenerated intervertebral disc and further

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Li, Yan-zhen, Hao-jie Xu, Jia-min Hu, et al. "Bioinformatic Analysis of Gene Expression Profile in Plasma of Hypertensive Patients." American Journal of Hypertension 33, no. 6 (2020): 581. http://dx.doi.org/10.1093/ajh/hpaa040.

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Abstract Background To analyze expression profiles of long noncoding RNA (lncRNA) and messenger RNA (mRNA) in patients with essential hypertension (EH) and normotensive adults. Methods The gene chip dataset GSE76845, which was generated from 5 plasma samples from patients with EH and 5 normotensives, was downloaded from the National Biotechnology Information Center Public Data Platform. Each sample (total RNA) was pooled from the total RNA of 3 age- and gender-matched subjects (EH patients or healthy controls). A ClusterProfiler package including gene set enrichment analysis (GSEA) was used to

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