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1

&NA;. "Tea-tree oil." Reactions Weekly &NA;, no. 400 (May 1992): 11. http://dx.doi.org/10.2165/00128415-199204000-00049.

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&NA;. "Tea tree oil." Reactions Weekly &NA;, no. 955 (June 2003): 14–15. http://dx.doi.org/10.2165/00128415-200309550-00046.

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&NA;. "Tea tree oil." Reactions Weekly &NA;, no. 959 (July 2003): 15. http://dx.doi.org/10.2165/00128415-200309590-00054.

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4

Larson, David, and Sharon E. Jacob. "Tea Tree Oil." Dermatitis 23, no. 1 (2012): 48–49. http://dx.doi.org/10.1097/der.0b013e31823e202d.

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5

Elliott, Charles. "Tea tree oil poisoning." Medical Journal of Australia 159, no. 11-12 (December 1993): 830–31. http://dx.doi.org/10.5694/j.1326-5377.1993.tb141370.x.

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Seawrlght, Alan. "Tea tree oil poisoning." Medical Journal of Australia 159, no. 11-12 (December 1993): 830–31. http://dx.doi.org/10.5694/j.1326-5377.1993.tb141371.x.

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7

Moss, Andrew. "Tea tree oil poisoning." Medical Journal of Australia 160, no. 4 (February 1994): 236. http://dx.doi.org/10.5694/j.1326-5377.1994.tb126628.x.

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8

de Groot, Anton C., and Erich Schmidt. "Eucalyptus oil and tea tree oil." Contact Dermatitis 73, no. 6 (July 15, 2015): 381–86. http://dx.doi.org/10.1111/cod.12450.

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9

Knight, Timothy E., and B. M. Hausen. "Melaleuca oil (tea tree oil) dermatitis." Journal of the American Academy of Dermatology 30, no. 3 (March 1994): 423–27. http://dx.doi.org/10.1016/s0190-9622(94)70050-8.

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10

Mallika, Linga, and Thanigaivel S. "Comparison of Antimicrobial and Antipathogenic Efficacy of Tea Tree Oil Nanoemulsion Against Gram Negative Pseudomonas Aeruginosa Infection in Cyprinus Carpio." ECS Transactions 107, no. 1 (April 24, 2022): 14027–36. http://dx.doi.org/10.1149/10701.14027ecst.

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The major aim of this study is to compare the antimicrobial and antipathogenic efficacy of tea tree oil nanoemulsion against gram negative Pseudomonas aeruginosa and invivo antibacterial efficacy of tea tree oil nanoemulsion in Cyprinus carpio. Materials and methods: Tea tree oil nanoemulsion (TTNE) preparation was done by the standard protocol. Tea tree oil nanoemulsion (N=21) and control antibiotics (Amoxicillin) and zone of inhibition was observed for the well and disc diffusion as per the procedure. Invivo efficacy of tea tree oil nanoemulsion in Cyprinus carpio was performed according to the standard procedure. Results: Independent sample T test was done which revealed that nano formulated tea tree oil showed comparatively good results against Pseudomonas aeruginosa with respect to the control antibiotics. The zone of inhibition in well diffusion was found to be 11 ±1mm for TT oil nanoemulsion and 17.25 ±1mm for antibiotic respectively. Nano formulated tea tree oil appears to have the MIC of 40µl and attained significance was (P<0.005). Conclusion: Tea tree oil nano emulsion showed better significant results than the antibiotics for zone of inhibition.
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11

Apted, John. "Sensitivity to Tea Tree Oil." Australasian Journal of Dermatology 33, no. 3 (December 1992): 158. http://dx.doi.org/10.1111/j.1440-0960.1992.tb00109.x.

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12

Ardiana, Dian. "Role Of Tea Tree Oil as A Skin Antimicrobial : A Literature Study." Medical and Health Science Journal 5, no. 1 (February 28, 2021): 26–33. http://dx.doi.org/10.33086/mhsj.v5i1.1921.

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Background: Skin disease due to microorganism infection are still widely found in community. The infections can be caused by bacteria, viruses, fungi, and parasite. Tea tree oil often used as a herbal medicine in the treatment of skin diseases due to microorganisms. This literature study is conducted to review the role of tea tree oil as an antimicrobial in skin infections. Method: Fifteen indexed journals published from 2015 to 2020 about tea tree oil and skin infections, were included. From 15 journals, 9 journals discuss antibacterial activity of tea tree oil, 2 journals discuss antiviral activity, 9 journals discuss antifungal activity, and 1 journal discusses antiparasitic activity. All journals state that tea tree oil has an antimicrobial effect on microorganisms that cause skin infections. Result: From 9 journals, it was found that A. baumanni, P. aeruginosa, and C. acnes were the most sensitive bacteria to tea tree oil in terms of MIC and S. epidermidis was the most sensitive bacteria, seen from their inhibition zone. Eight journals state variations with significant differences in the activity of tea tree oil as an anti-fungal. Tea tree oil has stronger antibacterial activity than antifungal activity. It also has antiviral activity against HSV and antiparasitic on S. scabiei. Conclusion: The conclusion of this study is that tea tree oil has antimicrobial activity against microorganisms that cause skin disease, including bacteria, viruses, fungi, and parasite.
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13

Yadav, Erosh, Sunil Kumar, Sheefali Mahant, Sarita Khatkar, and Rekha Rao. "Tea tree oil: a promising essential oil." Journal of Essential Oil Research 29, no. 3 (September 22, 2016): 201–13. http://dx.doi.org/10.1080/10412905.2016.1232665.

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14

Hammer, Katherine A., Christine F. Carson, and Thomas V. Riley. "Effects of Melaleuca alternifolia (Tea Tree) Essential Oil and the Major Monoterpene Component Terpinen-4-ol on the Development of Single- and Multistep Antibiotic Resistance and Antimicrobial Susceptibility." Antimicrobial Agents and Chemotherapy 56, no. 2 (November 14, 2011): 909–15. http://dx.doi.org/10.1128/aac.05741-11.

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ABSTRACTThis study examined the effect of subinhibitoryMelaleuca alternifolia(tea tree) essential oil on the development of antibiotic resistance inStaphylococcus aureusandEscherichia coli. Frequencies of single-step antibiotic-resistant mutants were determined by inoculating bacteria cultured with or without subinhibitory tea tree oil onto agar containing 2 to 8 times the MIC of each antibiotic and with or without tea tree oil. Whereas most differences in resistance frequencies were relatively minor, the combination of kanamycin and tea tree oil yielded approximately 10-fold fewer resistantE. colimutants than kanamycin alone. The development of multistep antibiotic resistance in the presence of tea tree oil or terpinen-4-ol was examined by culturingS. aureusandE. coliisolates daily with antibiotic alone, antibiotic with tea tree oil, and antibiotic with terpinen-4-ol for 6 days. Median MICs for each antibiotic alone increased 4- to 16-fold by day 6. Subinhibitory tea tree oil or terpinen-4-ol did not greatly alter results, with day 6 median MICs being either the same as or one concentration different from those for antibiotic alone. For tea tree oil and terpinen-4-ol alone, day 6 median MICs had increased 4-fold forS. aureus(n= 18) and 2-fold forE. coli(n= 18) from baseline values. Lastly, few significant changes in antimicrobial susceptibility were seen forS. aureusandS. epidermidisisolates that had been serially subcultured 14 to 22 times with subinhibitory terpinen-4-ol. Overall, these data indicate that tea tree oil and terpinen-4-ol have little impact on the development of antimicrobial resistance and susceptibility.
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15

Wulansari, Aprilla, Mahdi Jufri, and Angky Budianti. "STUDIES ON THE FORMULATION, PHYSICAL STABILITY, AND IN VITRO ANTIBACTERIAL ACTIVITY OF TEA TREE OIL (MELALEUCA ALTERNIFOLIA) NANOEMULSION GEL." International Journal of Applied Pharmaceutics 9 (October 30, 2017): 135. http://dx.doi.org/10.22159/ijap.2017.v9s1.73_80.

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Objective: This study aimed to formulate tea tree oil into a nanoemulsion gel dosage form and evaluate its physical stability and antibacterial activity.Methods: Nanoemulsion gels were formulated with various concentrations of tea tree oil, namely, 5%, 7%, and 9%, using Tween-80 as a surfactantand propylene glycol as a cosurfactant. The tea tree oil nanoemulsion gels showed a stable physical appearance over 8 weeks of storage at lowtemperature (4±2°C) and room temperature (25±2°C), cycling test, and centrifugation test.Results: The best formula was nanoemulsion gel formulation 1 (F1), which contained 5% tea tree oil, due to its good stability, smaller globule size,and greater viscosity. The results for antibacterial activity, determined by in vitro study, showed that the tea tree oil nanoemulsion gels exhibitedantibacterial activity against Propionibacterium acnes through the formation of an inhibition zone.Conclusion: Higher concentrations of tea tree oil in nanoemulsion gels (5%, 7%, and 9%) showed greater mean inhibition zones (28.33±0.88 mm,30.33±0.33 mm, and 31.67±0.33 mm, respectively).
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16

Riley, TV. "Topical antimicrobial therapy with tea tree oil: fact or fantasy." British Journal of Infection Control 3, no. 5 (October 2002): 12–15. http://dx.doi.org/10.1177/175717740200300503.

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T his paper reviews briefly the evidence that tea tree oil may be suitable as a topical antimicrobial agent. Tea tree oil is the essential oil extracted by steam distillation from the Australian native tree Melaleuca alternifolia. It has a long history of use in Australia, and has emerged as a possible topical antimicrobial because of antimicrobial resistance to conventional agents. The history, production and chemistry of tea tree oil are described. The in vitro antimicrobial activity of tea tree oil has now been reported in numerous papers, and there is good evidence for antibacterial, antifungal and, to a lesser extent, antiviral activity. What is lacking is sufficient numbers of clinical trials to verify this efficacy in vivo. Tea tree oil is only safe to use as a topical agent and although skin sensitivity has been a concern, the evidence for this is not strong. However, long-term usage studies are still required. Based on the published data, tea tree oil may prove to be a very useful topical antimicrobial and there is a role for infection control practitioners in the validation of its use in clinical practice.
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17

Spironello, Thaila Maria, Gisele Bandera, Janete Jacinta Lupatini Presser, Mariana Tortelli Beux, and Natália Freddo. "The benefits of melaleuca essential oil for acne treatment / Os benefícios do óleo essencial de melaleuca para o tratamento da acne." Brazilian Journal of Health Review 5, no. 3 (May 24, 2022): 10074–77. http://dx.doi.org/10.34119/bjhrv5n3-173.

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Acne is the most common disease that affects young people, among the various existing treatments, essential oils can be cited. Tea tree essential oil (Melaleuca alternifolia) also known as Tea Tree has medicinal actions that can improve acne degrees. The objective is to show the effectiveness of tea tree oil for the aesthetic treatments of acne, for this, bibliographic searches available on Google Scholar were carried out. Studies report that tea tree oil at a concentration of 5% can reduce the lesions caused by acne and its bacteria. The treatment aims to minimize the formation of scars and dyschromias, for that, it is noticed that the studies carried out have brought positive results and low adverse reactions in the treatment with the essential oil, before that the tea tree oil is used in cosmetic formulations helping in the treatments of acne.
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18

Concha, JM, LS Moore, and WJ Holloway. "1998 William J. Stickel Bronze Award. Antifungal activity of Melaleuca alternifolia (tea-tree) oil against various pathogenic organisms." Journal of the American Podiatric Medical Association 88, no. 10 (October 1, 1998): 489–92. http://dx.doi.org/10.7547/87507315-88-10-489.

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Tea-tree oil (oil of Melaleuca alternifolia) has recently received much attention as a natural remedy for bacterial and fungal infections of the skin and mucosa. As with most naturally occurring agents, claims of effectiveness have been only anecdotal; however, several published studies have recently demonstrated tea-tree oil's antibacterial activity. This study was conducted to determine the activity of tea-tree oil against 58 clinical isolates: Candida albicans (n = 10), Trichophyton rubrum (n = 8), Trichophyton mentagrophytes (n = 9), Trichophyton tonsurans (n = 10), Aspergillus niger (n = 9), Penicillium species (n = 9), Epidermophyton floccosum (n = 2), and Microsporum gypsum (n = 1). Tea-tree oil showed inhibitory activity against all isolates tested except one strain of E floccosum. These in vitro results suggest that tea-tree oil may be useful in the treatment of yeast and fungal mucosal and skin infections.
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19

Messager, Syndie, Katherine A. Hammer, Christine F. Carson, and Thomas V. Riley. "Sporicidal activity of tea tree oil." Australian Infection Control 11, no. 4 (December 2006): 112–21. http://dx.doi.org/10.1071/hi06112.

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20

&NA;. "Tea tree oil treats anaerobic vaginosis." Inpharma Weekly &NA;, no. 774 (February 1991): 7. http://dx.doi.org/10.2165/00128413-199107740-00017.

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21

Southwell, Ian A., Susanne Freeman, and Diana Rubel. "Skin Irritancy of Tea Tree Oil." Journal of Essential Oil Research 9, no. 1 (January 1997): 47–52. http://dx.doi.org/10.1080/10412905.1997.9700713.

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22

Bozzuto, Giuseppina, Marisa Colone, Laura Toccacieli, Annarita Stringaro, and Agnese Molinari. "Tea Tree Oil Might Combat Melanoma." Planta Medica 77, no. 01 (June 17, 2010): 54–56. http://dx.doi.org/10.1055/s-0030-1250055.

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23

De Groot, Anton C., and J. Willem Weyland. "Contact allergy to tea tree oil." Contact Dermatitis 28, no. 5 (May 1993): 309. http://dx.doi.org/10.1111/j.1600-0536.1993.tb03450.x.

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24

Ambrogio, Francesca, Caterina Foti, Gerardo Cazzato, Edoardo Mortato, Stella Mazzoccoli, Anna Paola De Caro, Nicoletta Cassano, Gino Antonio Vena, Gianfranco Calogiuri, and Paolo Romita. "Spreading Allergic Contact Dermatitis to Tea Tree Oil in an Over-the-Counter Product Applied on a Wart." Medicina 58, no. 5 (April 19, 2022): 561. http://dx.doi.org/10.3390/medicina58050561.

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Tea tree oil is an essential oil obtained by distillation from the leaves and terminal branchlets of Melaleuca alternifolia and is now present in numerous products for body care and self-medication. We report a case of allergic contact dermatitis to tea tree oil in a young man who was applying a lotion containing tea tree oil on a wart localized on the plantar aspect of the right big toe, which had previously been treated with cryotherapy. He developed a severe eczematous eruption on the right foot and the right leg, with subsequent id reactions affecting the right thigh, the contralateral lower limb, the trunk and the upper limbs. The lotion was discontinued, and the dermatitis resolved after topical corticosteroid therapy. Patch testing with the aforementioned lotion 10% pet. and oxidized tea tree oil 5% pet. identified tea tree oil as the culprit agent of the dermatitis. This case report confirms that products made of natural ingredients, often perceived to be harmless, can cause allergic reactions.
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Lakatos, Marietta, Samuel Obeng Apori, Julie Dunne, and Furong Tian. "The Biological Activity of Tea Tree Oil and Hemp Seed Oil." Applied Microbiology 2, no. 3 (July 25, 2022): 534–43. http://dx.doi.org/10.3390/applmicrobiol2030041.

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The interest in hemp seed oil (HSO) and tea tree oil (TTO) in the medical and food industries is increasing. The current study compares their bioactivity to other plant oils, mainly focusing on hemp seed oils (HSOs) with various cannabidiol (CBD) contents. A DPPH assay was employed to evaluate the antioxidant activity. The antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis was evaluated using time–kill, minimum inhibition concentration (MIC), and Kirby–Bauer disk diffusion methods. Tea tree oil showed significantly higher antimicrobial activity against S. enteritidis compared to E. coli and S. aureus (p < 0.05). The antioxitant activity range (lowest to highest) was sesame < vetiver < rosehip < tea tree < organic hemp < pure hemp < 5% CBD < vitamin C. Tea tree oil and 5% CBD showed antioxidant activity at IC50 of 64.45 μg/mL and 11.21 μg/mL, respectively. The opposing antimicrobial and antioxidant results for TTO and HSO indicate that these activities arise from different components within the oil compositions.
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Sheth, Hina, Uday Kamath, Sai Ramesh, and Keshav Singla. "Comparison of the Antibacterial Efficacy of Tea Tree Oil with 3% Sodium Hypochlorite and 2% Chlorhexidine against E. faecalis: An in vitro Study." Journal of Contemporary Dentistry 3, no. 3 (2013): 117–20. http://dx.doi.org/10.5005/jp-journals-10031-1049.

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ABSTRACT Background and objectives Among the bacterial species which most often cause endodontic failure, Enterococcus faecalis is the most important. This study compared the antibacterial efficacy of tea tree oil with 3% sodium hypochlorite and 2% chlorhexidine as a root canal irrigant, against E. faecalis Materials and Methods Normal saline, tea tree oil, 3% sodium hypochlorite, 2% chlorhexidine and fresh stains of E. faecalis were used. Four round wells, 4 mm deep and 8 mm diameter, were punched using sterile cork borer in blood agar plates and divided into four groups: group A: normal saline (control), group B: 3% sodium hypochlorite, group C: 2% chlorhexidine and group D: tea tree oil. Agar well diffusion method was performed to compare the antibacterial efficacy of tea tree oil, 3% sodium hypochlorite and 2% chlorhexidine. Results The results were tabulated and statistically analyzed using analysis of variance (ANOVA). Tea tree oil showed comparable inhibition of bacterial growth with sodium hypochlorite and chlorhexidine. No zone of inhibition was shown by normal saline (control). Interpretation and conclusion Tea tree oil showed statistically significant antimicrobial activity against Enterococcus faecalis, which is very much similar to sodium hypochlorite and chlorhexidine. Abbreviations CHX: Chlorhexidine; E. faecalis: Enterococcus faecalis; NaOCl: Sodium hypochlorite. How to cite this article Kamath U, Sheth H, Ramesh S, Singla K. Comparison of the Antibacterial Efficacy of Tea Tree Oil with 3% Sodium Hypochlorite and 2% Chlorhexidine against E. faecalis: An in vitro Study. J Contemp Dent 2013; 3(3):117-120.
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CHAUHAN, AMISHA, and SHIKHA BAGHEL CHAUHAN. "FORMULATION AND EVALUATION OF POLYHERBAL EMULGEL FOR TREATMENT OF ACNE." Current Research in Pharmaceutical Sciences 12, no. 3 (October 8, 2022): 132–37. http://dx.doi.org/10.24092/crps.2022.120302.

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Skin disease is a persistent inflammatory skin disorder that affects around 70% of teenage females throughout puberty. To counteract this problem, antibiotics are being administered more often, which has resulted in a variety of undesirable side effects. As a result, as a first-line topical therapy, it must target on the herbal formulation. In this study, medicinal plant extracts and oils with high antibacterial activity, such as Curcuma longa and Aloe barbadensis, tea tree oil, and lemongrass oil, were chosen to produce a polyherbal emulgel for the treatment of acne vulgaris. Curcuma longa, Aloe barbadensis, tea tree oil, and lemongrass oil were all extracted and reported in this study. Lemongrass oil, tea tree oil, aloe Vera extract, and turmeric extract were used in various amounts in the topical gels. After being kept at room temperature for 24 hours, the gel was prepared and evaluated. As a result of the study's findings, a prepared polyherbal emulgel containing extracts of Curcuma longa, lemon grass oil and tea tree oil at concentrations of 0.5 percent, 5 percent, 5 percent respectively, can be used to treat acne vulgaris. KEYWORDS: Polyherbal emulgel, Anti-acne formulation, Acne vulgaris, Curcuma longa, lemongrass oil, tea tree oil
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28

Lu, Cui, Li-Zhi Gao, and Qun-Jie Zhang. "A High-Quality Genome Assembly of the Mitochondrial Genome of the Oil-Tea Tree Camellia gigantocarpa (Theaceae)." Diversity 14, no. 10 (October 8, 2022): 850. http://dx.doi.org/10.3390/d14100850.

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Camellia gigantocarpa is one of the oil-tea trees whose seeds can be used to extract high-quality vegetable oil. To date, there are no data on the mitochondrial genome of the oil-tea tree, in contrast to the tea-tree C. sinensis, which belongs to the same genus. In this paper, we present the first complete mitochondrial genomes of C. gigantocarpa obtained using PacBio Hi-Fi (high-fidelity) and Hi-C sequencing technologies to anchor the 970,410 bp genome assembly into a single sequence. A set of 44 protein-coding genes, 22 non-coding genes, 746 simple sequence repeats (SSRs), and more than 201 kb of repetitive sequences were annotated in the genome assembly. The high percentage of repetitive sequences in the mitochondrial genome of C. gigantocarpa (20.81%) and C. sinensis (22.15%, tea tree) compared to Arabidopsis thaliana (4.96%) significantly increased the mitogenome size in the genus Camellia. The comparison of the mitochondrial genomes between C. gigantocarpa and C. sinensis revealed genes exhibit high variance in gene order and low substitution rate within the genus Camellia. Information on the mitochondrial genome provides a better understanding of the structure and evolution of the genome in Camellia and may contribute to further study of the after-ripening process of oil-tea trees.
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29

Bin Edris, Mohamad Adib, Awang Soh Yusuff Mamat, Muhammad Shahzad Aslam, and Muhammad Syarhabil Ahmad. "Insect Repellent Properties of Melaleuca alternifolia." Recent Advances in Biology and Medicine 02 (2016): 57. http://dx.doi.org/10.18639/rabm.2016.02.293742.

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The aim of this study is to compare the use of plant-based insect repellents that are environment friendly with the use of insect repellents based on chemical substances which can be harmful to the environment and human health. The plant studied here is “tea tree”; its scientific name isMelaleuca alternifolia. Essential oil from this plant is extracted by steam distillation method. Based on the previous research, tea tree oil has antimicrobial, antifungal, anti-inflammatory, and insect repellent properties. Some experiments were done on tea tree oil to determine its insect repellent properties and the suitable concentration that can be used to make sure its repelling effect is optimum. The purpose of this determination is to avoid its harmful effect on humans because it can be toxic if it is used at high concentration. The results showed that tea tree oil repelledTribolium castaneum. Furthermore, the toxicity assays also gave positive result where the tea tree oil has toxic properties againstSolenopsis invicta. The lethal dose (LD) of tea tree oil to kill 50% of a group ofS. invictais 23.52 μL/mL. This LD50 is determined by using the arithmetic method of Karber. Broadly, the results showed thatM. alternifoliahas insect repellent properties and shows toxicity against certain insects.
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30

Malik, Pooja, and Prashant Upadhyay. "GC-MS Chemical profile, Antioxidant Activity, and Sun Protection Factor of Essential Oil of Tea Tree (Melaleuca alternifolia) and Rosemary (Rosmarinus officinalis L.)." Oriental Journal Of Chemistry 38, no. 5 (October 31, 2022): 1266–75. http://dx.doi.org/10.13005/ojc/380524.

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The present investigation aimed to determine the oxidative potential, sun protection factor value, and half-maximal inhibitory concentration of rosemary essential oil and tea tree essential oil. These two essential oils have gained popularity as active ingredients in many cosmetic preparations due to their antioxidant activity, whether used individually or in combination. Gas chromatography-mass spectroscopy is used to identify the presence of different phytochemical constituents in essential oils. The GC-MS (Gas chromatography-mass spectroscopy) chemical analysis of tea tree oil revealed 34 and rosemary oil revealed 35 volatile chemical components with sesquiterpene hydrocarbon, monoterpenes (42.77%), and alcohols (41.01%) as major detected classes. The 2, 2-diphenylpicrylhydrazyl (DPPH) and nitric oxide-free scavenging activity techniques were used to investigate the antioxidant capacity of these oils. It was found that both tea tree and rosemary oil have the potential to slow down skin aging through their anti-oxidative properties using the approach of free radical scavenging activity. The UV spectroscopy method was used to determine the sun protection factor, and the sun protection values of rosemary and tea tree oil were found to be 8.45 and 6.85, respectively. Rosemary oil was an extremely promising tea tree essential oil for anti-aging and sunburn prevention. The study's findings indicated that rosemary and tea tree essential oil can both offer a synergistic sun protection factor effect, antioxidant property, and anti-aging or extra activity of cosmetic preparations.
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31

Riyankati, Baiq, Surya Hadi, and Sri Seno Handayani. "Characterization and Chemical Composition Analysis of Tea Tree (Meleuca alternifolia) Leaf Hydrosols Growing on Lombok Island." Jurnal Penelitian Pendidikan IPA 8, no. 1 (January 8, 2022): 119–23. http://dx.doi.org/10.29303/jppipa.v8i1.1239.

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Tea tree is an essential oil-producing plant from Australia which is also found growing in several parts of Indonesia, including the island of Lombok. So far, tea tree essential oil producers on the island of Lombok do not utilize by-products in the form of hydrosol (distilled water) produced in the process of making essential oils. In this study, the characterization and analysis of the chemical composition of the hydrosol of tea tree leaves growing on the island of Lombok was carried out. The characteristic aroma of hydrosol is similar to that of tea tree essential oil. The results of the GC-MS analysis also showed that the hydrosol of tea tree leaves also had similarities with the essential oil, composed of major compounds in the form of trans-caryophyllene (28.58%), limonene (13.98%) and terpinen-4-ol (16.27%). Other compounds detected were -pinene (4.14%), -pinene (6.50%), -myrcene (8.09%), -terpineol (10.10%) and -terpinene (5.77%).
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32

Hsu, Jui-Liang, Tzu-Yun Lin, Jung-Hul Chien, Chin-Hsien Hsu, Hsiao-Hsien Lin, and An-Chi Yeh. "A Study on the Release Persistence of Microencapsulated Tea Tree Essential Oil in Hotel Hot Spring Water." Water 14, no. 9 (April 26, 2022): 1391. http://dx.doi.org/10.3390/w14091391.

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To improve business performance and achieve sustainable development through the concept of hot spring resource reuse, this study investigated the antibacterial effect of alginate-coated tea tree essential oil microcapsules and the effect of alginate microcapsules on the release of tea tree essential oil. The results revealed that 450 μm alginate/tea tree essential oil microcapsules (containing 720 ppm of tea tree essential oil) prepared using microfluidic assemblies effectively inhibited total bacteria, Escherichia coli, and Staphylococcus aureus in hot spring water. For alginate/tea tree essential oil microcapsules prepared under different conditions, at a fixed concentration of cross-linking reagents, the release time increased with the cross-linking time (10 min > 5 min > 1 min). At a fixed cross-linking time, the release time increased with the concentrations of cross-linking reagents (1 M > 0.5 M > 0.1 M). When the concentrations of cross-linking reagents and the cross-linking time were the same, the release time of cross-linking reagents increased with the strength of metal activity (Ca > Zn).
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33

Capetti, Francesca, Barbara Sgorbini, Cecilia Cagliero, Monica Argenziano, Roberta Cavalli, Luisella Milano, Carlo Bicchi, and Patrizia Rubiolo. "Melaleuca alternifolia Essential Oil: Evaluation of Skin Permeation and Distribution from Topical Formulations with a Solvent-Free Analytical Method." Planta Medica 86, no. 06 (February 25, 2020): 442–50. http://dx.doi.org/10.1055/a-1115-4848.

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Abstract Melaleuca alternifolia essential oil (tea tree oil) is widely used as an ingredient in skin care products because of its recognized biological activities. The European Scientific Committee on Consumer Products constantly promotes research and collection of data on both skin distribution and systemic exposure to tea tree oil components after the application of topical formulations. This study quantitatively evaluates permeation, skin layer distribution (stratum corneum, epidermis, and dermis), and release into the surrounding environment of bioactive tea tree oil markers (i.e., α-pinene, β-pinene, α-terpinene, 1,8-cineole, γ-terpinene, 4-terpineol, α-terpineol) when a 5% tea tree oil formulation is applied at a finite dosing regimen. Permeation kinetics were studied in vitro on pig ear skin using conventional static glass Franz diffusion cells and cells ad hoc modified to monitor the release of markers into the atmosphere. Formulation, receiving phases, and skin layers were analyzed using a fully automatic and solvent-free method based on headspace solid-phase microextraction/gas chromatography-mass spectrometry. This approach affords, for the first time, to quantify tea tree oil markers in the different skin layers while avoiding using solvents and overcoming the existing methods based on solvent extraction. The skin layers contained less than 1% of each tea tree oil marker in total. Only oxygenated terpenes significantly permeated across the skin, while hydrocarbons were only absorbed at trace level. Substantial amounts of markers were released into the atmosphere.
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Thosar, Nilima, Silpi Basak, Rakesh N. Bahadure, and Monali Rajurkar. "Antimicrobial efficacy of five essential oils against oral pathogens: An in vitro study." European Journal of Dentistry 07, S 01 (September 2013): S071—S077. http://dx.doi.org/10.4103/1305-7456.119078.

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ABSTRACT Objectives: This study was aimed to find out the minimum inhibitory concentration (MIC) of five essential oils against oral pathogens and to find out the minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) of five essential oils against oral pathogens. Materials and Methods: The antimicrobial activities by detecting MIC and MBC/MFC of five essential oils such as tea tree oil, lavender oil, thyme oil, peppermint oil and eugenol oil were evaluated against four common oral pathogens by broth dilution method. The strains used for the study were Staphylococcus aureus ATCC 25923, Enterococcus fecalis ATCC 29212, Escherichia coli ATCC 25922 and Candida albicans ATCC 90028. Results: Out of five essential oils, eugenol oil, peppermint oil, tea tree oil exhibited significant inhibitory effect with mean MIC of 0.62 ± 0.45, 9.00 ± 15.34, 17.12 ± 31.25 subsequently. Mean MBC/MFC for tea tree oil was 17.12 ± 31.25, for lavender oil 151.00 ± 241.82, for thyme oil 22.00 ± 12.00, for peppermint oil 9.75 ± 14.88 and for eugenol oil 0.62 ± 0.45. E. fecalis exhibited low degree of sensitivity compared with all essential oils. Conclusion: Peppermint, tea tree and thyme oil can act as an effective intracanal antiseptic solution against oral pathogens.
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Maftuchah, Maftuchah, Priskila Iris Christine, and M. Jamaluddin. "The Effectiveness of Tea Tree Oil and Eucalyptus Oil Aromaterapy for Toddlers with Common Cold." JURNAL KEBIDANAN 10, no. 2 (October 28, 2020): 131–37. http://dx.doi.org/10.31983/jkb.v10i2.6360.

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Common cold is a mild viral infection of the upper respiratory tract, nose, and throat. Common cold suffered by each toddler in Indonesia is predicted to occur three to six times a year, which means that a toddler may experience three to six times of coughs and colds every year. Common cold is also a symptom of Covid-19 whose early treatments used symptomatic and non-pharmacological therapy including tea tree oil and eucalyptus oil aromatherapy. Related to this, this paper reveals the effectiveness of tea tree oil and eucalyptus oil aromatherapy for the healing period of 1 to 2-year-old toddlers in T W Independent Midwifery Practice (IMP). This paper employs quasy experiment design with non-equivalent control group model. Sample includes 14 toddlers with the age of 1 to 2-year-old. Seven of them were given tea tree oil, while the rest were given eucalyptus oil aromatherapy with accidental sampling technique. Aromatherapy was given once a day for seven days. Findings showed that the healing period of the control and intervention group was four to five days and according to the Mann Whitney trial, p-value of 0,530 (0,05) was obtained. In conclusion, there is no difference between the healing period of common cold using tea tree oil and eucalyptus oil aromatherapy for 1 to 2-year-old toddlers in T W Independent Midwifery Practice (IMP). Tea tree oil and eucalyptus oil aromatherapy can be used to accelerate the healing period of common cold suffered by toddlers.
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36

Ahmed, Qasim. "EVALUATION OF TEA TREE EXTRACT FORMULATION FOR THE CONTROL OF THE COTTON APHID, APHIS GOSSYPII (HOMOPTERA: APHIDIDAE) ON CAPSICUM ANNUUM IN THE GLASSHOUSE." Journal of Biopesticides 15, no. 1 (June 1, 2022): 31–38. http://dx.doi.org/10.57182/jbiopestic.15.1.31-38.

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Under laboratory and glasshouse conditions, the insecticidal activities of the essential oil obtained from leaves of the tea tree plant by steam distillation was evaluated against the cotton aphid, Aphis gossypii. The results showed that tea tree essential oil affected the aphid population causing a higher mortality of A. gossypii. This study showed that the 50 μL/mL concentration of tea essential oil caused 85.67% mortality after 24 hrs exposure time when used as contact treatment. However, the mortalities at 10, 20 and 30 μL/mL were 18.33, 21.67 and 70%, respectively, after 24 hrs of exposure. Conversely, the residual treatment was the most effective on the cotton aphid, with 100% mortality at the concentration of 50 μL/mL after 24 hrs of exposure to the essential oil. In contrast, the mortalities of A. gossypii were 15.00, 24.44 and 92.78% at the concentrations of 10, 20 and 30 μL/mL, respectively, after 24 hrs of exposure. The formulation of the tea tree essential oil was enormously potent in reducing the cotton aphid population on the sweet pepper, but was slightly phytotoxic to the potted plants. No significant differences were observed between tea tree oil formulations compared with the chemical pesticides, Karate and botanical insecticide, Levo. Based on our findings, the tea tree essential oil can be used as a botanical pesticide against A. gossypii after modifying it to reduce the phytotoxicity on the plants.
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37

Thomson, Natalie, Katherine Hammer, and Thomas Riley. "Tea-tree oil – a naturally occurring biocide." Microbiology Australia 31, no. 4 (2010): 182. http://dx.doi.org/10.1071/ma10182.

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Tea-tree oil (TTO) is the essential oil derived from an Australian native plant that has been used for hundreds of years, mainly for its antimicrobial and anti-inflammatory properties. With the advent of ever-increasing resistance to antibiotics and biocides amongst organisms, TTO may play an important role in the health industry in the fight against resistance.
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38

&NA;. "Tea tree oil may find clinical application." Inpharma Weekly &NA;, no. 991 (June 1995): 20. http://dx.doi.org/10.2165/00128413-199509910-00039.

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39

Zhang, Si Yi, and Donald Robertson. "A Study of Tea Tree Oil Ototoxicity." Audiology and Neurotology 5, no. 2 (2000): 64–68. http://dx.doi.org/10.1159/000013869.

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40

Carson, Christine F., and Thomas V. Riley. "The antimicrobial activity of tea tree oil." Medical Journal of Australia 160, no. 4 (February 1994): 236. http://dx.doi.org/10.5694/j.1326-5377.1994.tb126627.x.

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41

Minghetti, Paola, Antonella Casiraghi, Francesco Cilurzo, Veniero Gambaro, and Luisa Montanari. "Formulation Study of Tea Tree Oil Patches." Natural Product Communications 4, no. 1 (January 2009): 1934578X0900400. http://dx.doi.org/10.1177/1934578x0900400129.

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The antimicrobial, antifungal and anti-inflammatory properties of tea tree oil (TTO), the essential oil of Melaleuca alternifolia are well documented. In order to optimize its therapeutic activity, TTO patches were designed. The aim of this work was the formulation of monolayer patches containing TTO. Moreover, the performance of oleic acid (OA) as a skin penetration enhancer in patches was evaluated. Terpinen-4-ol (T4OL), the main component of TTO, was the marker used to evaluate TTO skin permeability. The permeation study was performed through human epidermis by using Franz diffusion cells. Patches were prepared by using methacrylic copolymers, Eudragit E100 (EuE100) or Eudragit NE (EuNE), and a silicone resin, BioPSA7-4602 (Bio-PSA). TTO and OA contents were fixed at 10% w/w and 3% w/w, respectively. The patches were prepared by a casting method and characterised in terms of T4OL content and skin permeability. All the selected polymers were suitable as the main component of the patch matrix. Since the main critical issue in the use of TTO is related to its toxicity after absorption, the local administration of TTO can take advantage of the use of patches based on EuE100 because of the high retained amount and the low permeation of T4OL. In this matrix, OA slightly increased the T4OL retained amount, improving the efficacy and safety of TTO patches.
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42

Wallengren, Joanna. "Tea tree oil attenuates experimental contact dermatitis." Archives of Dermatological Research 303, no. 5 (September 24, 2010): 333–38. http://dx.doi.org/10.1007/s00403-010-1083-y.

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43

Blackwell, A. L. "Tea tree oil and anaerobic (bacterial) vaginosis." Lancet 337, no. 8736 (February 1991): 300. http://dx.doi.org/10.1016/0140-6736(91)90910-h.

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44

Mills, Clive, Brian V. Cleary, John J. Walsh, and John F. Gilmer. "Inhibition of acetylcholinesterase by Tea Tree oil." Journal of Pharmacy and Pharmacology 56, no. 3 (March 2004): 375–79. http://dx.doi.org/10.1211/0022357022773.

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45

De Groot, Anton C., and J. Willem Weyland. "Systemic contact dermatitis from tea tree oil." Contact Dermatitis 27, no. 4 (April 1992): 279–80. http://dx.doi.org/10.1111/j.1600-0536.1992.tb03279.x.

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46

Hammer, K. "In-vitro activity of essential oils, in particular Melaleuca alternifolia (tea tree) oil and tea tree oil products, against Candida spp." Journal of Antimicrobial Chemotherapy 42, no. 5 (November 1, 1998): 591–95. http://dx.doi.org/10.1093/jac/42.5.591.

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47

Greig, Jane E., Siew-Lee Thoo, Christine F. Carson, and Thomas V. Riley. "Allergic contact dermatitis following use of a tea tree oil hand-wash not due to tea tree oil." Contact Dermatitis 41, no. 6 (December 1999): 354–55. http://dx.doi.org/10.1111/j.1600-0536.1999.tb06197.x.

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48

Markovic, D., B. Mirkovic, T. Jovanovic, A. Knezevic, and T. Nastovski. "The application of tea tree essential oil in dentistry." Serbian Dental Journal 54, no. 2 (2007): 106–14. http://dx.doi.org/10.2298/sgs0702106m.

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Essential oils are widely used in medicine, dentistry and cosmetology as flavour and odour corrigents in various substances for oral hygiene. The aim of this study was to present comprehensively the possibilities for application of Melaleuca Alternifolia essential oil in dentistry based on the analysis of contemporary scientific and professional publications. The application of Tea tree essential oil in the treatment of periodontal, fungal and viral diseases is very efficient. The study of antimicrobial potential of ten different essential oils confirmed the efficiency of Tea tree oil against numerous Gram+ and Gram- bacteria. In vitro studies of bacteriostatic, bactericidal and fungicidal effect of Tea tree oil solution against ten different microorganisms confirmed sensitivity of the following microorganisms: Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum and Porphyromonas gingivalis, and slightly weaker effect against Streptococcus Mutans and Prevotella intermedia. Tea tree is very effective in the treatment of various diseases and is an introduction and momentum for the application of plant substances in the treatment of numerous diseases in dentistry. .
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49

Varvani Farahani, Pouran, Davood Hekmatpou, Hadi Jafarimanesh, Pegah Matoripour, and Mehdi Harorani. "Comparing the Effect of Tea Tree Oil and Lavender on Bacterial Samples of Nurses’ Hands." Complementary Medicine Journal 11, no. 4 (January 1, 2022): 292–303. http://dx.doi.org/10.32598/cmja.11.4.18.3.

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Objective: One of the most typical ways of transmitting the infection is by hand; thus, much emphasis has been placed on hand hygiene. In this regard, this study aimed to determine the effect of tea tree oil and lavender on bacterial samples found in nurses’ hands. Methods: This quasi-experimental study was performed on all nurses working in ICU wards. The samples were selected by an easy non-probability sampling method. The research tools were demographic information questionnaires and a bacterial culture registration form. In the intervention phase, on the first day in the first experimental group, 2 drops of lavender oil, on the second day, 2 drops of tea tree oil, and on the third day, 2 drops of distilled water were poured on the nurse’s palm, and their hands were massaged for 10 seconds. Before and after the intervention, culture samples were taken from each group. The obtained data were analyzed with the Chi-square test, paired t test, and ANOVA in SPSS software v. 20 at a significant level of less than 0.05. Results: The most common organisms isolated at the beginning of the study were Staphylococcus epidermidis (76.8%), Staphylococcus aureus (8.8%), Klebsiella spp. (7.9%), and Escherichia coli (6.5%). The use of lavender oil and tea tree has been effective in reducing the microbial load of the palm. Hand massage with lavender oil had less effect on reducing germs (P=0.003), but tea tree oil had a more significant reduction of pathogens (P=0.001). Conclusion: Lavender and tea tree oil reduced the microbial load of nurses. Both methods have been effective, but the effect of tea tree oil was more significant. Therefore, their use in handwashing is recommended to eliminate palm bacteria in ICU staff.
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Saoud, Amer Ali, Rosli Mohd Yunus, and Ramlan Abd Aziz. "Yield Study of Tea Tree Essential Oil Using Microwave-Assisted Process." ASEAN Journal of Chemical Engineering 6, no. 1 (June 1, 2006): 22. http://dx.doi.org/10.22146/ajche.50150.

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There have been developments on the use of microwave to accelerate the digestion of solid materials, the heating of materials, and, more recently, the extraction of solute from solids via solvent as extracting medium. Microwave-assisted process (MAP) was used to accelerate the extraction of target compounds. It can be used for the extraction of compounds from various plant and animal tissues or of undesirable components from raw materials. Tea tree leaves were used in this study to investigate the applicability of microwave irradiation for essential oil extraction. The microwave parameters studied were tea tree leaves/ethanol ratio as well as required dose of microwave and time of irradiation. Different ratios of tea tree leaves/ethanol had been examined in order to obtain the optimal feed/solvent ratio that would give the highest yield of extracted essential oil. The required number of microwave doses that provided an accomplished extraction process had been ascertained. The optimal time of microwave exposure was found to be at 3 min. The measurements of extracted tea tree essential oil constituents (Le., cineole, a-pinene, and y-terpinene) that represent the major constituents were performed using gas chromatography (GC) analysis to estimate the yield of extracted tea tree essential oil.
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