Relevant bibliographies by topics / Techniques de knock-out de gènes

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Techniques de knock-out de gènes'

Author: Grafiati
Published: 21 September 2024
Last updated: 31 July 2025

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Journal articles on the topic "Techniques de knock-out de gènes"

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Wolf, Steven E., and Kenneth J. Woodside. "Transgenic and gene knock-out techniques and burn research." Journal of Surgical Research 123, no. 2 (2005): 328–39. http://dx.doi.org/10.1016/j.jss.2004.06.001.

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Monahan, Paul E. "Factor IX: Insights from knock-out and genetically engineered mice." Thrombosis and Haemostasis 100, no. 10 (2008): 563–75. http://dx.doi.org/10.1160/th08-04-0262.

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SummaryThe study of coagulation factors has been rapidly advanced by studies performed in genetically engineered mouse strains. Investigation of factor IX (FIX) has benefited from excellent genedeleted mouse models that recapitulate many of the features of human haemophilia B. Moreover, advanced positional cloning techniques and availability of technology to allow not only knock-out mice, but also knock-in and knock-down mice, provide new opportunities to observe genotype-phenotype and structure-function correlations regarding FIX, as well as the interaction of FIX with inflammatory, immune, a
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Ohara, Hiroki, and Toru Nabika. "Genetic Modifications to Alter Blood Pressure Level." Biomedicines 10, no. 8 (2022): 1855. http://dx.doi.org/10.3390/biomedicines10081855.

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Genetic manipulation is one of the indispensable techniques to examine gene functions both in vitro and in vivo. In particular, cardiovascular phenotypes such as blood pressure cannot be evaluated in vitro system, necessitating the creation of transgenic or gene-targeted knock-out and knock-in experimental animals to understand the pathophysiological roles of specific genes on the disease conditions. Although genome-wide association studies (GWAS) in various human populations have identified multiple genetic variations associated with increased risk for hypertension and/or its complications, t
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Yang, Caiting, Yu Lei, Tinglin Ren, and Mingze Yao. "The Current Situation and Development Prospect of Whole-Genome Screening." International Journal of Molecular Sciences 25, no. 1 (2024): 658. http://dx.doi.org/10.3390/ijms25010658.

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High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function genetic screening is mainly achieved through RNA interference (RNAi), CRISPR knock-out (CRISPRko), and CRISPR interference (CRISPRi) technologies. Gain-of-function genetic screening mainly depends on the overexpression of a cDNA library and CRISPR activation (CRISPRa). Base editing can perform both gain- and loss-of-function genetic screening. This review discusses genetic screening techniques based on Cas9 nuclease, including Cas
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Syarifuddin, Syarifuddin, Ade Ulansari, and Iftahurrahmah Iftahurrahmah. "ARCHITECTURAL DESIGN AND WOODEN SRUCTURES OF TRADITIONAL OGAN ILIR BUILDINGS AS THE LOCAL CULTURAL WEALTH OF THE OGAN ILIR COMMUNITY." Sosiohumaniora 24, no. 2 (2022): 256. http://dx.doi.org/10.24198/sosiohumaniora.v24i2.36893.

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South Sumatra has a lot of traditional houses across its region. Each house has its own characteristics. One notable design is houses built on stilts. In Ogan Ilir, especially Tanjung Batu, it is famous for the production of knock-down houses. Knock-down houses in Tanjung Batu are a stilt house with an architectural design that almost resembles a Limas house, only that these knock-down houses are specially designed to make it easy to disassemble and reassemble them elsewhere. This study aims to give a closer look at the design, structure and wood choices of knock-down houses that have become u
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Riyandi, Ages, and Desy Misnawati. "STRATEGI PEMASARAN PRODUK RUMAH KNOCK DOWN PADA MASYARAKAT TANJUNG BATU SEBERANG." Jurnal Inovasi 15, no. 1 (2021): 11–27. http://dx.doi.org/10.33557/ji.v15i1.2200.

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The production of knock down houses is in a great demand especially in Tanjung BatuSeberang.The craft of knxok down houses was passed down from the era of Sriwijaya Kingdom untilnow. The demand of the craft is inscreasing from year to year this has turned out to be anopportunity so that many young people are engaged in this business. Kotler and Armstrong'sMarketing Strategy Theory (2004 in Varadarajan 2009). The research method uses qualitativeresearch. Collecting data through observation as the main source, interviews and documentation.While data analysis techniques, data presentation and dat
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Mittal, Vikram. "A Review of Recent Advancements in Knock Detection in Spark Ignition Engines." Signals 5, no. 1 (2024): 165–80. http://dx.doi.org/10.3390/signals5010009.

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In gasoline engines, the combustion process involves a flame’s propagation from the spark plug to the cylinder walls, resulting in the localized heating and pressurization of the cylinder content ahead of the flame, which can lead to the autoignition of the gasoline and air. The energy release from the autoignition event causes the engine cylinder to resonate, causing an unpleasant noise and eventual engine damage. This process is termed as knock. Avoiding knock has resulted in limiting the maximum engine pressures, and hence limiting the maximum efficiencies of the engine. Modern engines empl
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Fabbri, Mattia, and Pier Giuseppe Giribone. "Design, implementation and validation of advanced lattice techniques for pricing EAKO — European American Knock-Out option." International Journal of Financial Engineering 06, no. 04 (2019): 1950032. http://dx.doi.org/10.1142/s2424786319500324.

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The paper presents a series of advanced lattice methods aimed at evaluating an EAKO European-American Knock-Out contract. The first part of the paper deals with the numerical methods implemented for pricing: Binomial and Trinomial Stochastic trees, Adaptive Mesh Model, Pentanomial and Heptanomial lattice. In the second part, specific tests are designed to validate the code written in Matlab language. The study concludes by applying the most performing model to a real market case.
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Balla, Beata, Florin Tripon, and Claudia Banescu. "From Descriptive to Functional Genomics of Leukemias Focusing on Genome Engineering Techniques." International Journal of Molecular Sciences 22, no. 18 (2021): 10065. http://dx.doi.org/10.3390/ijms221810065.

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Genome engineering makes the precise manipulation of DNA sequences possible in a cell. Therefore, it is essential for understanding gene function. Meganucleases were the start of genome engineering, and it continued with the discovery of Zinc finger nucleases (ZFNs), followed by Transcription activator-like effector nucleases (TALENs). They can generate double-strand breaks at a desired target site in the genome, and therefore can be used to knock in mutations or knock out genes in the same way. Years later, genome engineering was transformed by the discovery of clustered regularly interspaced
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Li, Meng, Xufang Niu, Shuang Li, et al. "CRISPR/Cas9 Based Cell-Type Specific Gene Knock-Out in Arabidopsis Roots." Plants 12, no. 12 (2023): 2365. http://dx.doi.org/10.3390/plants12122365.

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CRISPR/Cas9 (hereafter Cas9)-mediated gene knockout is one of the most important tools for studying gene function. However, many genes in plants play distinct roles in different cell types. Engineering the currently used Cas9 system to achieve cell-type-specific knockout of functional genes is useful for addressing the cell-specific functions of genes. Here we employed the cell-specific promoters of the WUSCHEL RELATED HOMEOBOX 5 (WOX5), CYCLIND6;1 (CYCD6;1), and ENDODERMIS7 (EN7) genes to drive the Cas9 element, allowing tissue-specific targeting of the genes of interest. We designed the repo
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Dissertations / Theses on the topic "Techniques de knock-out de gènes"

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Chaumont, Lise. "Functional study of key fish interferon-stimulated genes using an in vitro knock-out approach in fish cell lines : from comparative immunology to interest for vaccine production." Electronic Thesis or Diss., université Paris-Saclay, 2024. http://www.theses.fr/2024UPASL038.

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Chez les vertébrés à mâchoires, les défenses antivirales innées sont principalement basées sur les interférons (IFN) de type I. Ces cytokines sont sécrétées en cas d'infection virale et induisent l'expression de gènes stimulés par l'IFN (ISGs). Les ISGs codent des protéines aux fonctions diverses dont l'expression conduit à l'établissement d'un état réfractaire à l'infection. Le système IFN de type I est globalement bien conservé entre les mammifères et les poissons, mais le répertoire des ISGs est plus diversifié chez ces derniers, en raison de leur histoire évolutive complexe et de leurs spé
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Liu, Junjun. "Métabolisme du Tryptophane et fonctions de la cellule Bêta pancréatique en condition normale et au cours du diabète." Sorbonne Paris Cité, 2015. http://www.theses.fr/2015USPCC083.

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Dans le système nerveux central, la voie tryptophane/kynurénine (T/K) est considérée maintenant comme un acteur majeur de la physiopathologie des maladies neurodégénératives et psychiatriques associées à une inflammation chronique. Le diabète de type Ilest aussi associé à une activation chronique de l'immunité innée qui installe une inflammation chronique à bas bruit au niveau des îlots pancréatiques. Comme les cellules bêta pancréatiques partagent un grand nombre de similitudes avec les cellules neuronales, les îlots pancréatiques représententun nouveau tissu au sein duquel la voie T/K pourra
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Helary, Louise. "Validation in vivo de l'implication de nouveaux gènes impliqués dans le développement musculaire des mammifères." Thesis, Limoges, 2019. http://www.theses.fr/2019LIMO0053.

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Même si les acteurs majeurs du développement musculaire ont été identifiés et les voies de transductions décrites, d’autres régulateurs restent encore à découvrir. Un crible ARNi pratiqué sur un modèle cellulaire couramment utilisé, la lignée myoblastique C2C12, a identifié 20 nouveaux gènes potentiellement impliqués dans la myogenèse in vitro. Au cours de ma thèse, deux de ces gènes ont été invalidés sur modèle souris en utilisant la technologie CRISPR/Cas9 pour valider in vivo leur implication. Pour l’un d’entre eux, seuls les animaux hétérozygotes ont pu être étud
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Maï, Wilfried. "Diagnostic phénotypique des souris transgéniques et knock-out par les techniques d'imagerie haute résolution." Lyon 1, 2004. http://www.theses.fr/2004LYO10101.

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L'imagerie haute résolution des rongeurs de laboratoire s'est largement développée au cours des dernières années pour répondre aux besoins des chercheurs, demandeurs de techniques non invasives d'étude des centaines de modèles d'animaux transgéniques et knock-out actuellement disponibles. Les défis techniques étaient nombreux, mais les outils existent aujourd'hui. Dans cette thèse, l'auteur illustre les applications de l'échographie haute résolution à l'exploration non invasive des souris trangéniques dans différentes applications : en biologie moléculaire dans un modèle de souris déficiente e
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Dougier, Hei-lanne. "Commutation de classe et effet néo sur les interactions de longue distance dans le locus des chaînes lourdes d'immunoglobulines." Limoges, 2005. http://aurore.unilim.fr/theses/nxfile/default/d8b1a7d6-1b90-4369-9441-7f148b54763d/blobholder:0/2005LIMO0025.pdf.

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Le gène néo(R) est couramment utilisé comme marqueur de sélection dans les expériences de recombinaison homologue. Mais il est également utilisé comme révélateur des mécanismes sous-jacents aux interactions de longue distance dans les loci complexes comme le locus IgH. Dans ce contexte, mon travail de thèse a porté sur les perturbations générées par le remplacement de séquences du locus IgH par le marqueur néoR. Le premier modèle correspond à des souris mutantes dans lesquelles la quasi-totalité de l'intron Iµ-Cµ (mais laissant intactes les séquences nécessaires à un épissage correct des pré-m
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Odelin, Gaëlle. "Etude du rôle du facteur de transcription Krox20 dans le développement et la maturation des valves cardiaques chez la souris." Thesis, Aix-Marseille, 2015. http://www.theses.fr/2015AIXM5014.

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Les pathologies valvulaires aortiques sont des pathologies plurifactorielles, comportant un déterminisme génétique indiscutable mais peu caractérisé. Ma thèse a pour but d’étudier le rôle du facteur de transcription Krox20 au cours du développement et de la maturation valvulaire à travers l’analyse de modèles murins. Nous avons montré que ce gène est nécessaire au développement et à la maturation de la valve aortique. L’invalidation de Krox20 chez la souris conduit à une hypertrophie des feuillets aortiques dès les stades fœtaux et à des insuffisances aortiques chez l’adulte. Ces anomalies son
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Duflaut, Dominique. "Etudes moléculaires et fonctionnelles des gènes TSAP6 (Tumor Suppressor Activated Pathway 6) et TCTP (Translationally Controlled Tumor Protein) : cibles pharmacologiques anti-tumorales." Phd thesis, Université Paris Sud - Paris XI, 2008. http://tel.archives-ouvertes.fr/tel-00293914.

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L'objectif de mon travail de doctorat était une caractérisation fonctionnelle des gènes TCTP et TSAP6. Ces gènes sont impliqués dans la réversion tumorale et forment un complexe protéique. <br />Tpt1, codant pour la protéine TCTP, est le gène le plus sous-exprimé lors de la réversion tumorale. L'analyse du cristal de TCTP humain montre une forte homologie entre ses hélices H2-H3 et les hélices H5-H6 de Bax. Grâce à ses hélices, TCTP inhibe l'apoptose induite par Bax au niveau des mitochondries. En effet, nous démontrons que TCTP, entre autres par le biais de ces hélices, empêche la dimérisatio
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Book chapters on the topic "Techniques de knock-out de gènes"

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Lashkevich, Katsiaryna, Lino Moises Mediavilla Ponce, Manuel Camargo, Fredrik Milani, and Marlon Dumas. "Discovery of Improvement Opportunities in Knock-Out Checks of Business Processes." In Lecture Notes in Business Information Processing. Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-33080-3_23.

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AbstractOverprocessing is a source of waste that occurs when unnecessary work is performed in a process. Overprocessing is often found in application-to-approval processes since a rejected application does not add value, and thus, work that leads to the rejection constitutes overprocessing. Analyzing how the knock-out checks are executed can help analysts to identify opportunities to reduce overprocessing waste and time. This paper proposes an interpretable process mining approach for discovering improvement opportunities in the knock-out checks and recommending redesigns to address them. Expe
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"Knock-out of Genes in Yeast." In Techniques for Molecular Biology. CRC Press, 2006. http://dx.doi.org/10.1201/9781482294460-55.

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Hancock, John T. "A look at some of the history and techniques of cell signalling." In Cell Signalling. Oxford University Press, 2016. http://dx.doi.org/10.1093/hesc/9780199658480.003.0003.

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This chapter focuses on the history of cell signalling and provides an overview of some of the main techniques that could be used to study cell signalling. It presents a wide range of technologies which can be adopted for signalling studies: the use of antibodies, fluorescent probes and confocal microscopy, and traditional biochemical approaches. The chapter also looks at how the development of modern molecular biology techniques furthered our understanding of how many of these proteins function through the use of gene sequence analysis, overexpression, and knock-out and knock-down studies. It
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Houdebine, Louis Marie. "Chapter 6.3 Ethical implications of knock-out and transgenesistechniques for animal research." In Handbook of Molecular-Genetic Techniques for Brain and Behavior Research. Elsevier, 1999. http://dx.doi.org/10.1016/s0921-0709(99)80069-8.

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Schroten, Egbert. "Ethical Aspects of Genetic Modification of Animals: Opinion of the Group of Advisers on the Ethical Implications of Biotechnology of the European Commission." In The Cloning Source Book. Oxford University PressNew York, NY, 2001. http://dx.doi.org/10.1093/oso/9780195128826.003.0025.

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Abstract The opinion addresses the genetic modification of animals brought about by DNA technology, including transfer of genes (transgenesis) and the deletion of genes (“knock-out”).Genetic modification of animals is a developing technology which will add to rather than replace existing techniques.
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Hedou, Gael, and Isabelle M. Mansuy. "Inducible molecular switches for the study of long-term potentiation." In Long-term Potentiation: Enhancing Neuroscience for 30 years. Oxford University PressOxford, 2004. http://dx.doi.org/10.1093/oso/9780198530305.003.0025.

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Abstract This article reviews technical and conceptual advances in unravelling the molecular bases of long-term potentiation (LTP), learning and memory using genetic approaches. We focus on studies aimed at testing a model suggesting that protein kinases and protein phosphatases balance each other to control synaptic strength and plasticity. We describe how gene ’knock-out’ technology was initially exploited to disrupt the Ca2+ /calmodulin-dependent protein kinase Ilc.t (CaMKifo) gene and how refined knock-in techniques later allowed an analysis of the role of distinct phosphorylation sites in
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Mccarrick, James W., and Peter W. Andrews. "Embryonal carcinoma cells and embryonic stem cells as models for neuronal development and function." In Neuronal Cell Lines. Oxford University PressOxford, 1992. http://dx.doi.org/10.1093/oso/9780199633463.003.0004.

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Abstract For many years teratocarcinomas and their stem cells, embryonal carcinoma (EC) cells, have been well established tools for investigating cell differentiation as it relates to early embryogenesis, especially in the mouse (1, 2). More recently, lines of human EC cells have been characterized to extend this approach to human embryogenesis (3), while the isolation of embryonic stem (ES) cells (4, 5) has opened up important new approaches for the study of gene function during embryonic and fetal development. Many general aspects concerning the use of such cells were extensively covered in
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Ramdev, Poornima Devi C., Divya K. Shankar, and B. Renuka. "CRISPR-Cas for Genome Editing - Molecular Scissors for Combating Pathogens." In Genome Editing in Bacteria (Part 2). BENTHAM SCIENCE PUBLISHERS, 2024. http://dx.doi.org/10.2174/9789815223798124010005.

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Clustered Regularly Interspaced Short Palindromic Repeats, abbreviated as CRISPR, is a genome-editing technology that permits the creation of precise knock-out mutants by aiding the modification of gene sequences devoid of the steps involving the insertion of foreign DNA into pathogenic microorganisms. The microorganisms are ubiquitous in nature and harbor in the complex ecosystem of the human being. Cas (acronym for CRISPR-associated) genes are present in many microbial genomes. The variable nature of the microbial genome has been utilized as an integral typing tool in epidemiologic, diagnost
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Conference papers on the topic "Techniques de knock-out de gènes"

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Forte, Claudio, Enrico Corti, and Gian Marco Bianchi. "Combined Experimental and Numerical Analysis of Knock in Spark Ignition Engines." In ASME 2009 Internal Combustion Engine Division Fall Technical Conference. ASMEDC, 2009. http://dx.doi.org/10.1115/icef2009-14102.

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A detailed analysis of knocking event can help improving engine performance and diagnosis strategies. The paper aim is a better understanding of the phenomena involved in knocking combustion through the combination of CFD and signals analysis tools. CFD simulations have been used in order to reproduce knock effect on the in-cylinder pressure trace. In fact, the in-cylinder pressure signal holds information about waves propagation and heat losses: for the sake of the diagnosis it is important to relate knock severity to knock indexes values. For this purpose, a CFD model has been implemented, a
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Tonelli, Roberto, Massimo Medda, Fabrizio Gullino, et al. "Experimental and Numerical Investigation of Different Dilution Techniques in a High-Performance H2 - Fueled SI Engine." In WCX SAE World Congress Experience. SAE International, 2025. https://doi.org/10.4271/2025-01-8424.

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&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;Nowadays, hydrogen (H2) is rising as a key solution to fuel internal combustion engines (ICE) since it allows carbon free combustion process. At the same time, ICE fueled with H2 can reach similar performance and driving experience of gasoline fueled ones. In stoichiometric conditions, hydrogen shows higher flame speed, lower ignition energy and lower quenching distance than gasoline. Mainly for these reasons, H2 combustion is characterized by a high risk of abnormal combustion (i.e. knock and pre-ignition), relevant NOx
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Mahmoud, Ahmed M., Bunyen Teng, S. Jamal Mustafa, and Osama M. Mukdadi. "High-Frequency Ultrasound Tissue Classification of Atherosclerotic Plaques in an APOE-KO Mouse Model Using Spectral Analysis." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-13061.

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Small animal models have been widely used in cardiovascular research when studying the development and treatment of different diseases. This kind of research has promoted the development of noninvasive techniques to assess cardiac tissue and blood vessels of small animals. Recently, we have developed a high-frequency ultrasound imaging system for small animals, in particular, mouse and rat models. In this work, we aim to elucidate the usefulness of using spectral analysis of the received radiofrequency (RF) ultrasound signals to extract quantitative parameters to assess mechanical properties o
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Singh, Inderpal, Arne Güdden, Ankit Raut, et al. "Experimental and Numerical Investigation of a Single-Cylinder Methanol Port-Fuel Injected Spark Ignition Engine for Heavy-Duty Applications." In Symposium on International Automotive Technology. SAE International, 2024. http://dx.doi.org/10.4271/2024-26-0072.

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&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;With the increasing focus on reducing CO&lt;sub&gt;2&lt;/sub&gt; emissions to combat global warming and climate change, the automotive industry is exploring near zero-emission alternative fuels to replace traditional fossil-based fuels like diesel, gasoline, and CNG. Methanol is a promising alternative fuel that is being evaluated in India due to its easy transportation and storage, as well as its production scalability and availability potential. This study focuses on the retro-fitment solution of M100 (pure methanol) S
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Ciatti, Stephen, Swami Nathan Subramanian, and Alison Ferris. "Effect of EGR in a Gasoline Operated Diesel Engine in LTC Mode." In ASME 2012 Internal Combustion Engine Division Spring Technical Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/ices2012-81010.

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Conventional combustion techniques struggle to meet the current emissions’ regulations while retaining high engine efficiency. Specifically in automotive diesel engines, oxides of nitrogen (NOx) and particulate matter (PM) emissions have limited the utilization of diesel fuel in compression ignition engines. By comparison, throttled, knock-limited conventional gasoline operated SI engines tend not to be fuel efficient. Advanced combustion systems that simultaneously address PM and NOx while retaining the high efficiency of modern diesel engines, are being developed around the globe [1]. One of
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