Relevant bibliographies by topics / Teeth Dental therapeutics / Dissertations / Theses
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Dissertations / Theses on the topic 'Teeth Dental therapeutics'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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1

Lang-Hua, Bich Hue, and 梁許碧蕙. "Decision-making in dental treatment planning: to maintain or to extract compromised teeth." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B50534282.

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Background: A number of systematic reviews provide the basis for decision-making in treatment planning of the reconstruction of mutilated dentitions. This includes identifying teeth difficult to treat/save and decisions on replacement such teeth if extracted. Aims: 1) To assess attitudes of general dental practitioners (GDPs) in a community where provision of dental implants is a widely practiced treatment; and to determine variations in attitudes with respect to dentists’ factors, training factors and implant-provision factors (Study 1): and 2) To determine treatment decision making with respect to maintaining periodontally compromised teeth among dentists with and without postgraduate qualifications in implant dentistry (Study 2). Methods: Study 1:A cross-sectional survey was conducted on a random sample of registered dentists in Hong Kong regarding their attitudes towards implant dentistry with respect to (1) perceived superiority, (2) perceived outcomes, (3) perceived complications and maintenance issues and (4) placement issues. In addition, information was collected on dentists’ factors, training factors and implant provision factors. Variations in attitudes towards implant dentistry were explored. Study 2: A series ofpatient scenarios with varying degrees of periodontal disease levels was presented to selected dentists. Information on their decision-making outcomes, and their intention to retain compromised teeth was analysed in bivariate and regression analyses; accounting for postgraduate implant training, gender, years in dental practice and implant placement experience. Results: Study 1: Among eligible practitioners (n=246), the response rate was 46.3%. Most dentists’perceived implants to be superior to conventional prostheses for the replacement of a single missing posterior tooth (80%) and likewise, for the replacement of a single missing anterior tooth (67%). Variations in attitudes existed with respect to dentists’ factors: years in practice (p<0.05), place of graduation (p<0.05); implant training factors (p<0.05), number of days of training (p<0.05) and implant experience factors (p<0.05). Study 2:This study involved 30 dentists with postgraduate implant qualifications (GDPP), 33 dentists without postgraduate implant qualifications (GDP) and 27 dentists undergoing postgraduate training for implant qualifications (GDPT). Variations in treatment decision-making were evident between the three groups (p<0.05). Differences in treatment approaches to retaining compromised teeth were apparent(p<0.05). Furthermore, variations in rehabilitation of extracted scenarios existed in terms of use of implants and number of implants needed for rehabilitation. Accounting for dentist and practice factors in regression analyses, compared to GDP, GDPP/GDPT were three times as likely to retain periodontally compromised upper molars with painwith pain (OR 3.08, 95%CI 1.09, 8.14 p=0.03), or without pain (OR 3.10, 95%CI 1.04, 10.62 p=0.04). Conclusions: Study 1:In a community where provision of dental implants is widespread the attitudes of GDPs are not wholly in line with evidence-based knowledge. Variations in their attitudes exist with respect to dentist factors, training and experience issues. Study 2: Variations in treatment decision making with respect to retaining periodontally compromised teeth exist between dentists with and dentists without postgraduate training in implant dentistry. Furthermore, there are differences in their management approaches.<br>published_or_final_version<br>Dentistry<br>Master<br>Master of Philosophy
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2

Lam, Garret Chi Yan. "Biomechanics of orthodontic tooth movement /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?MECH%202003%20LAM.

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Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2003.<br>Includes bibliographical references (leaves 118-122). Also available in electronic version. Access restricted to campus users.
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3

Ribeiro, Erica Del Peloso. "Iodo povidine associado a instrumentação periodontal no tratamento de lesões de bifurcação : avaliações clinica e bioquimica." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/287918.

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Orientadores: Marcio Zaffalon Casati, Sergio de Toledo<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba<br>Made available in DSpace on 2018-08-04T05:05:03Z (GMT). No. of bitstreams: 1 Ribeiro_EricaDelPeloso_M.pdf: 1796294 bytes, checksum: 9bd1ed49b1a4f14b073dca3cf4bbb7e2 (MD5) Previous issue date: 2005<br>Resumo: O objetivo do presente estudo foi avaliar a ação de uma solução de iodo povidine (PVP-I), associada à raspagem e alisamento radicular, no tratamento de lesões de bifurcação. Foram selecionados 44 pacientes com pelo menos um molar com lesão de bifurcação classe II, em face livre, profundidade de sondagem ³ 5 mm e sangramento à sondagem. Os pacientes foram divididos aleatoriamente em 2 grupos: grupo controle - raspagem e alisamento radicular com ultra-som e água destilada como solução refrigerante; grupo teste - raspagem e alisamento radicular com ultra-som e PVP-I 10% como solução refrigerante. Foram avaliados os seguintes parâmetros clínicos: índice de placa (IP), sangramento à sondagem (SS), posição da margem gengival (PMG), nível clínico de inserção relativo (NICr), profundidade de sondagem (PS) e nível clínico de inserção horizontal relativo (NICHr). A avaliação bioquímica da atividade de enzimas tipo tripsina no biofilme subgengival foi feita pelo teste BAPNA. Os parâmetros descritos acima foram avaliados antes do tratamento, 1, 3 e 6 meses após. Ambos os grupos apresentaram médias semelhantes de redução da PS, ganho de NICr e de NICHr. Aos 6 meses, esses valores foram, respectivamente, 2,31 mm, 1,17 mm e 1,00 mm no grupo controle e 2,31 mm, 1,23 mm e 1,02 mm no grupo teste (p>0,05). Diferença estatisticamente significante também não foi observada entre os grupos quanto ao número de sítios que ganharam 2 mm ou mais de inserção. Aos 6 meses, os grupos controle e teste apresentaram, respectivamente, 45,16% e 30,77% das áreas com ganho de inserção = 2 mm. Na comparação entre os grupos quanto ao SS das lesões de bifurcação aos 6 meses e à quantidade de áreas que exigiram retratamento no 3° mês foi encontrado p=0,06, favorecendo o grupo teste. O teste BAPNA não detectou diferença entre os grupos, entretanto, foi observada diferença intra-grupo, indicando redução na atividade de enzimas do tipo tripsina. Pôde-se concluir que o PVP-I, usado como adjunto da instrumentação periodontal traz benefícios clinicamente significantes ao tratamento de lesões de bifurcação classe II, em faces livres<br>Abstract: The aim of this randomized single-blind controlled clinical trial was to evaluate the effect of topically applied povidone-iodine (PVP-I), used as an adjunct to non-surgical therapy of furcation involvements. Forty-four patients presenting at least one class II furcation involvement that bled on probing, in bucal or lingual surfaces, with probing pocket depth ³ 5 mm were recruited. Patients were stratified into 2 treatment groups: subgingival instrumentation by an ultrasonic device using PVP-I (10%) as the cooling liquid (test group); identical treatment using distilled water as the cooling liquid (control group). The following clinical outcomes were evaluated: plaque index, bleeding on probing (BOP), position of the gingival margin, relative attachment level (RAL), probing pocket depth (PPD) and relative horizontal attachment level (RHAL). BAPNA test was used to analysis the trypsin-like activity in dental biofilm. The clinical and biochemical parameters were evaluated at baseline and 1, 3 and 6 months after therapy. Both groups had similar means of PPD reduction and RAL and RHAL gain. At 6 months, these variables were, respectively, 2.31 mm, 1.17 mm and 1.00 mm in the control group and 2.31 mm, 1.23 mm and 1.02 mm in the test group (p<0.05). No differences were observed in terms of the frequency distribution of sites demonstrating RAL gain ³ 2 mm. The comparison between groups on BOP of the furcation sites, at 6 months and on the number of sites referred for re-treatment at 3 months showed better values in the test group (p=0.06). The results of BAPNA test failed to demonstrate significant differences between groups. The use of topically applied PVP-I with subgingival instrumentation may provide additional clinical significant benefits to the treatment of class II furcation involvements<br>Mestrado<br>Periodontia<br>Mestre em Clínica Odontológica
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Heiden, Katia Regina. "Avaliação de doses em órgãos radiossensíveis expostos durante exames de tomografia computadorizada por feixe cônico e radiografia panorâmica." Universidade Tecnológica Federal do Paraná, 2016. http://repositorio.utfpr.edu.br/jspui/handle/1/2647.

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A radiografia panorâmica e a tomografia computadorizada por feixe cônico (TCFC) são métodos de imagem amplamente utilizados na Odontologia, pois fornecem uma visão geral do complexo dentomaxilofacial, sendo importantes no diagnóstico e tratamento de lesões que acometem esta região. A radiografia panorâmica é considerada um procedimento de baixa dose e a TCFC apresenta doses inferiores quando comparada à tomografia computadorizada (TC). Apesar de baixas, as doses provenientes destes exames expõem órgãos radiossensíveis, como tireoide e glândulas salivares, aos riscos da radiação ionizante. Conhecer os níveis de radiação à que estes órgãos estão expostos é importante para a escolha adequada do método de imagem e parâmetros de exposição a serem utilizados, reduzindo os riscos associados à radiação ionizante, conforme o princípio de radioproteção ALARA (As Low As a Reasonably Achievable - tão baixo quanto razoavelmente exequível). O objetivo deste estudo foi avaliar as doses absorvidas por estes órgãos radiossensíveis durante a realização de radiografias panorâmicas e exames de TCFC, utilizando diferentes parâmetros de exposição. Dosímetros termoluminescentes (TLDs - Thermoluminescent Dosimeters) foram inseridos em um phantom antropomórfico Alderson Radiation Therapy (ART) para identificar as doses absorvidas pela tireoide e glândulas salivares, em um equipamento de radiografia panorâmica e dois equipamentos de TCFC, com diferentes protocolos. As doses absorvidas variaram de 0,01 a 0,22 mGy na tireoide e de 0,26 a 2,96 mGy nas glândulas salivares. Na TCFC as doses variaram de acordo com o protocolo selecionado, porém foram superiores às doses de uma radiografia panorâmica. Os protocolos de TCFC que utilizaram longos tempos de exposição, considerados como protocolos de alta resolução, resultaram em doses superiores quando comparados aos protocolos que utilizaram baixos tempos de exposição. Protocolos que utilizaram campos de visão (FOVs - Fields Of View) maiores, expuseram a tireoide a níveis mais altos de radiação. Diante dos resultados obtidos, pode-se concluir que a TCFC somente deve ser solicitada caso a informação diagnóstica não possa ser obtida através da radiografia panorâmica, sendo a escolha adequada dos fatores técnicos imprescindível para reduzir a dose de radiação durante a realização de exames de TCFC.<br>Panoramic radiograph and cone beam computed tomography (CBCT) are imaging methods widely used in dentistry because they provide an overview of dentomaxillofacial complex, being important in the diagnosis and treatment of lesions that affect this region. Panoramic radiograph is considered a low-dose procedure and CBCT presents lower doses when compared with computed tomography (CT). Although low, the doses from these imaging methods expose radiosensitive organs, such as thyroid and salivary glands, to the risks of ionizing radiation. Knowing the levels of radiation these organs are exposed is important to select the appropiate imaging method and exposure parameters to be used, reducing the risks associated with ionizing radiation, according to the principle of radioprotection ALARA (As Low As a Reasonably Achievable). The aim of this study was to evaluate the absorbed doses by these radiosensitive organs during the course of panoramic radiographs and CBCT examinations, using different exposure parameters. Thermoluminescent dosimeters (TLDs) were inserted into an anthropomorphic phantom Alderson Radiation Therapy (ART) to identify the absorbed doses by the thyroid and salivary glands, in a panoramic x-ray unit and two CBCT units with different protocols. Absorbed doses ranged from 0.01 to 0.22 mGy in the thyroid and 0.26 to 2.96 mGy in the salivary glands. Doses in CBCT varied according to the selected protocol, but were higher than the dose of a panoramic radiograph. The CBCT protocols that used long exposure times, considered as high-resolution protocols, resulted in higher doses compared to protocols that used low exposure times. Protocols that used fields of view (FOVs) higher, exposed the thyroid at higher levels of radiation. Based on the results obtained, it can be concluded that CBCT should only be requested if the diagnostic information can’t be obtained through the panoramic radiograph and the appropriate choice of technical factors essential to reduce the radiation dose during examinations CBCT.
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PAIVA, PRISCILA F. "Laser diagnostico e tratamento da carie dental: uma visao clinica." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10930.

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Mei, Lei, and 梅蕾. "Actions of chlorhexidine and silver diamine fluoride on cariogenic biofilm and root caries." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44900776.

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7

BACHMANN, LUCIANO. "Estrutura e composicao do esmalte e da dentina tratados termicamente ou irradiados com lasers emissores no infravermelho." reponame:Repositório Institucional do IPEN, 2004. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11179.

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EBEL, PATRICIA. "Estudo in vitro do efeito do laser de diodo sobre a superficie de esmalte dental humano desmineralizado artificialmente." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11114.

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ROCHA, DALVA M. "Avaliacao clinica e morfologica da acao do laser de Er:YAG frente a hipersensibilidade dentinaria cervical." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10865.

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BARROSO, MARCIA C. da S. "Estudo 'in vitro' da acao do led e laser de diodo no clareamento dental." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11082.

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MATTOS, ALESSANDRA de S. "Avaliacao 'in vitro' das alteracoes quimica e morfologica da superficie do esmalte utilizando diferentes tecnicas de clareamento dental." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11090.

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QUINTO, JUNIOR JOSE. "Estudo in vitro do esmalte dental irradiado com laser de diodo de alta potencia em 960nm: analise morfologica da superficie dental pos irradiada e analise do comportamento termico na camara pulpar devido a aplicacao laser." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10983.

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MACRI, RODRIGO T. "Avaliacao da variacao da temperatura na camara pulpar apos a irradiacao de diodo laser de alta potencia de 830 nanometros em esmalte dental: estudo in vitro." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10965.

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OLIVEIRA, RODRIGO G. de. "Avaliacao da permeabilidade e da alteracao morfologica da superficie dentinaria apos apicectomia, tratamento e retropreparo com os lasers de Er:YAG e Nd:YAG." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10860.

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DALL, MAGRO EDUARDO. "Resistencia de uniao ao cisalhamento de dois sistemas adesivos em superficies dentinarias preparadas com laser de ER:YAG." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10861.

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CIASCA, THATYANA C. "Controle de parametros de irradiacao laser para selamento de tubulos dentinarios." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11527.

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SUN, CHIEN YUAN. "Analise comparativa do efeito da irradiacao do laser de GaAlAS em 780 nm e 660 nm na hipersensibilidade dentinaria." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11096.

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GODOY, BRUNO M. "Efeitos da radiacao laser de GaAlAs (lambda=660nm) em baixa intensidade na interface dentina-polpa pos preparo cavitario classe I." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11117.

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RIBEIRO, RAFAEL C. "Analise 'in vivo' do emprego laser de Er:YAG e do metodo convencional para a remocao do tecido cariado." reponame:Repositório Institucional do IPEN, 2002. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11045.

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SILVEIRA, MARIA C. G. da. "Efeitos da radiacao laser de vapor de cobre na parede de dentina de canais radiculares: Estudo in vitro por meio de microscopia eletronica de varredura e microscopio estereoscopico." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10866.

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SALLES, ALINE P. de O. "Modelagem dos efeitos termicos e opticos na camara pulpar durante a irradiacao do laser de Nd:YAG no tratamento da hipersensibilidade dentinaria." reponame:Repositório Institucional do IPEN, 2005. http://repositorio.ipen.br:8080/xmlui/handle/123456789/9399.

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PULGA, FABIANE G. "Estudo clinico comparativo entre o uso de lasers e metodos convencionais de diagnostico e tratamento de dentes deciduos da presenca de lesao cariosa." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10981.

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MAGALHAES, MANOELITA F. de. "Estudo in vitro do uso do laser de Nd:YAG na permeabilidade dentinaria cervical." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10982.

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MOURA, ABILIO A. M. de. "Avaliacao morfologica da superficie dentinaria e cementaria apos apicectomia com broca Zekrya e com o laser de Er:YAG e irradiacao direta e indireta com o laser de Nd:YAG." reponame:Repositório Institucional do IPEN, 2002. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11054.

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ARCHILLA, JOSE R. de F. "Analise termica de diferentes tecnicas de utilizacao do laser de Nd:YAG apos o preparo quimico-cirurgico de dentes unirradiculares." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10929.

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PICININI, LEONARDO S. "Avaliacao da temperatura na camara pulpar durante preparo classe V com laser de erbio:YAG." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10992.

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GLAUCHE, CARLOS E. C. "laser de Nd:YAG associado a solucoes de sais metalicos no tratamento da hipersensibilidade dentinaria." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10926.

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RODRIGUES, LUCIANE B. "Avaliacao das alteracoes morfologicas da superficie do preparo cavitario apos condicionamento com verniz fluoretado a 2,26 porcento e laser de Er:YAG atraves de microscopia eletronica de varredura." reponame:Repositório Institucional do IPEN, 2002. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11042.

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JUNQUEIRA, JUNIOR DUILIO N. "Estudo da influencia da angulacao do feixe laser na morfologia de esmalte e dentina irradiados com laser de Er:YAG." reponame:Repositório Institucional do IPEN, 2002. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11052.

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MARTINS, VINICIUS R. G. "Avaliacao da temperatura intrapulpar de incisivos bovinos irradiados com o laser de ER:YAG." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11092.

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CARVALHO, BRENO C. F. de. "Avaliacao do clareamento exogeno sobre a dentina realizado pela tecnica por ativacao quimica comparada com a tecnica por ativacao pelo laser do diodo." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11118.

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BLAY, CLAUDIA C. "Analise comparativa da reducao bacteriana com irradiacao do laser Er:YAG ou ponta montada em alta rotacao apos remocao de tecido cariado em dentina: estudo in anima nobile." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10940.

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NEGRAO, JOSE A. de S. "Efeitos do Er:YAG laser na cirurgia periapical microscopia eletronica de varredura." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10927.

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SEBRAO, CATIA C. N. "Avaliacao da microinfiltracao apical de condutos radiculares irradiados in vitro com laser de ER:YAG." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11091.

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THEODORO, LETICIA H. "Estudo comparativo, atraves de microscopia eletronica de varredura, da acao de diferentes substancias quimicas e do laser de Er:YAG, na remocao de smear layer, em superficies radiculares submetidas a raspagem e aplainamento." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10867.

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CARVALHO, WENDELL L. de. "Avaliacao in vitro da microinfiltracao marginal em restauracoes de classe V com resina composta em dentes bovinos. Influencia da irradiacao laser e sistema adesivo no pre-tratamento dentinario." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11108.

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MOREIRA, ALEXANDRE A. "Avaliacao da adesao do cimento de ionomero de vidro a dentina irradiada com laser de Nd:YAG." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10977.

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MARRACCINI, TARSO M. "Estudo comparativo do condicionamento do esmalte e dentina para a adesao de resinas compostas com os lasers Er:YAG 2,94 micrometros e com o laser Co sub(2) de 9,6 micrometros." reponame:Repositório Institucional do IPEN, 2002. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11030.

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ROMANO, ANA C. C. C. "Avaliacao in vitro das alteracoes morfologicas da superficie da dentina radicular irradiada pelo laser de COsub(2) associado ou nao a aplicacao de hidroxido de calcio." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11084.

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CALMON, ANDREA de A. "Estudo comparativo morfologico da acao do laser de ND:YAG em dentina cariada de dentes deciduos in vitro, condicionados com diamino fluoreto de prata a 12 porcento e carvao mineral." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10945.

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SCHEIN, MARCELO T. "Avaliacao, atraves de microscopia eletronica de varredura, do padrao de interacao dentina-resina em cavidades preparadas com laser de Er:YAG." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10976.

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Broady, Adam B. "Effectiveness of ozonated water irrigation against an established Enterococcus faecalis biofilm in root canal treated teeth in vitro." Thesis, 2020. http://hdl.handle.net/1805/23181.

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Indiana University-Purdue University Indianapolis (IUPUI)<br>Introduction: One of the main objectives of endodontic therapy is to reduce microbes and remove inflamed pulpal tissue within the root canal system (RCS). This is accomplished through chemomechanical debridement of the RCS using hand and rotary instrumentation along with an antimicrobial irrigant. Today, the most commonly used irrigant is sodium hypochlorite (NaOCl), often at concentrations toxic to human cells. The use of ozone as an endodontic irrigant is a novel technique that has been proven to be antimicrobial against several microorganisms. However, independent research is lacking on ozone’s efficacy against an established endodontic biofilm. If ozone’s efficacy against biofilms is confirmed, the use of toxic and potentially dangerous sodium hypochlorite could be replaced in some clinical situations (i.e., regeneration, immature teeth, resorption) with a safer and effective alternative. Objective: The aim of the current study was to evaluate the anti-biofilm activity of different concentrations of ozonated water compared to various concentrations of NaOCl against an established endodontic biofilm of Enterococcus faecalis in root canal treated teeth in vitro. Materials and Methods: The crowns of similarly sized, maxillary anterior teeth were removed, and the roots cut to a standard length (12 mm). All root canals were instrumented to a standard size. Specimens were sterilized and then inoculated with E. faecalis, which were allowed to grow for two weeks to form an established biofilm. There were six treatment groups: 1) 6% NaOCl; 2) 1.5% NaOCl; 3) 16µg/mL ozonated water; 4) 25µg/mL ozonated water; 5) 50µg/mL ozonated water, and 6) saline. Following treatment, samples were collected, plated, and incubated for two days. The number of CFU/mL were determined, and samples visualized using confocal imaging. The effect of treatment group on bacterial counts was made using one-way ANOVA followed by pair-wise comparisons. Null Hypothesis: Endodontically treated teeth irrigated with ozonated water will not demonstrate a statistically significant decrease in the E. faecalis biofilm compared to those treated with sodium hypochlorite Results: CFUs were converted to log10 and compared using Fisher’s Exact tests or one-way ANOVA followed by pair-wise tests. In all observations utilizing NaOCl irrigation, no colonies formed following treatment. The two NaOCl groups, with 0 CFU/mL, were significantly different than the other four groups (p=0.009). Saline showed a trend towards higher CFU/mL than 50 µg/ml O3 (p=0.068). None of the other comparisons approached statistical significance (p=0.453 25 µg/ml O3, p=0.606 16 µg/ml O3, p=0.999 25 µg/ml O3 vs 50 µg/ml O3, p=0.990 16 µg/ml O3 vs 50 µg/ml O3, p=1.000 16 µg/ml O3 vs 25 µg/ml O3). Confocal imaging helped illustrate effects of irrigation and confirm CFU findings. Conclusion: The results of this study failed to reject the null hypothesis. There was a statistically significant difference in the E. faecalis biofilm remaining in the groups treated with ozonated water compared to those treated with NaOCl. However, there was a trend towards higher CFU/mL in the saline group compared to the 50µg/mL ozonated water group. According to this finding, future studies should evaluate the effects of higher concentrations of ozonated water against an established E. faecalis biofilm. In addition, other follow-up studies might include ozonated water’s effect on human cells, such as the stem cells of the apical papilla that are so critical to the success of regenerative endodontic procedures. Due to university and laboratory closures caused by the COVID-19 pandemic, this project was stopped short and an insufficient sample size did not allow for proper statistical power. Additional occasions should be run upon the university’s re-opening to allow for proper statistical power.
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