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1

Al-Amery, Samah M., Phrabhakaran Nambiar, Jacob John, et al. "Unusual Dental Morphology in a Chimpanzee: A Case Report Utilizing Cone-Beam Computed Tomography." Journal of Veterinary Dentistry 35, no. 2 (2018): 96–102. http://dx.doi.org/10.1177/0898756418776448.

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This case report illustrates the teeth morphology of a chimpanzee and its anatomical variations. A well-preserved skull of a male Pan troglodytes troglodyte chimpanzee was scanned using a cone-beam computed tomography machine. Measurements included tooth and crown height, root length, root canal length and width (posterior teeth), and pulp cavity length (anterior teeth). Nonmetrical parameters included number of canals and foramina per root of every root. Interestingly, the mandibular central incisor was longer than the lateral incisor, and all the mandibular anterior teeth presented with a solitary flame-shaped or conical-calcified structure in their pulp cavity. The premolars are usually dual rooted except for the first maxillary premolar that displayed 3 roots. Other unusual discoveries were the presence of bilateral radicular dens invaginatus in the mandibular first premolars and the possibility of having 2 canals and 2 foramina in the roots of the posterior teeth. The presence of conical stone mineralizations at the pulp cavity and the presence of dens invaginatus were of particular interest.
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2

Likitpongpipat, Nattriya, Somboon Sangmaneedet, Poramaporn Klanrit, Rajda Noisombut, Suttichai Krisanaprakornkit, and Pattama Chailertvanitkul. "Promotion of Dental Pulp Wound Healing in New Zealand White Rabbits’ Teeth by Thai Propolis Product." Journal of Veterinary Dentistry 36, no. 1 (2018): 17–24. http://dx.doi.org/10.1177/0898756418818891.

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This study examined and compared wound healing between Thai propolis product and calcium hydroxide paste as pulp-capping agents after partial pulpotomy in New Zealand white rabbits. Forty incisor teeth from 10 rabbits were treated. Thirty-six teeth received class V cavity preparations with partial pulpotomy and application of either propolis or calcium hydroxide paste. Similar cavity preparations were performed in 2 teeth without any capping material as a positive control, whereas 2 teeth without the cavity preparation served as a negative control. Histological evaluation showed that both groups had dentin bridge formation. Dentinal tubules in the dentin bridge were more orderly arranged in the Thai propolis group than in the calcium hydroxide group. Wound healing and the median number of hyperemic blood vessels were not statistically significant different between the 2 groups. Thai propolis product may be used as a pulp-capping agent.
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3

Nakamura, Y., L. Hammarström, E. Lundberg, et al. "Enamel Matrix Derivative Promotes Reparative Processes in the Dental Pulp." Advances in Dental Research 15, no. 1 (2001): 105–7. http://dx.doi.org/10.1177/08959374010150010201.

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During odontogenesis, amelogenins from the preameloblasts are translocated to differentiating odontoblasts in the dental papilla, suggesting that amelogenins may be associated with odontoblast changes during development. In the present study, we have explored the effects of enamel matrix derivative (EMD) on the healing of a pulpal wound. Coronal pulp tissue of permanent maxillary premolars of miniature swine were exposed through buccal class V cavities. The exposed pulp was capped with EMD. The contralateral teeth served as controls and were capped with a calcium hydroxide paste (Dycal®). The cavities were sealed with glass-ionomer cement. After 2 and 4 weeks, the histology of the teeth was analyzed. In the EMD-treated teeth, large amounts of newly formed dentin-like hard tissue with associated formative cells outlined the pulpal wound separating the cavity area from the remaining pulp tissue. Inflammatory cells were present in the wound area but not subjacent to the newly formed hard tissue. Morphometric analysis showed that the amount of hard tissue formed in EMD-treated teeth was more than twice that of the calcium-hydroxide-treated control teeth (p < 0.001), suggesting that EMD is capable of promoting reparative processes in the wounded pulp more strongly than is calcium hydroxide.
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4

Barbosa, Antonio Vinicius Holanda, Gerhilde Callou Sampaio, Fábio Almeida Gomes, Daniel Pinto de Oliveira, Diana Santana de Albuquerque, and Ana Paula Veras Sobral. "Short-Term Analysis of Human Dental Pulps After Direct Capping with Portland Cement." Open Dentistry Journal 3, no. 1 (2009): 31–35. http://dx.doi.org/10.2174/1874210600903010031.

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This study evaluated the short-term response of human pulp tissue when directly capped with Portland cement. In this series of cases, twenty human third molars that were scheduled for extraction were used. After cavity preparation, pulp exposure was achieved and Portland cement pulp capping was performed. Teeth were extracted after 1, 7, 14 and 21 days following treatment and prepared for histological examination and bacterial detection. Each group had 5 teeth. The results were descriptively analysed. Dentin bridge formation was seen in two teeth with some distance from the material interface (14 and 21 days). Soft inflammatory responses were observed in most of the cases. Bacteria were not disclosed in any specimen. PC exhibited some features of biocompatibility and capability of inducing mineral pulp response in short-term evaluation. The results suggested that PC has a potential to be used as a less expensive pulp capping material in comparison to other pulp capping materials.
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5

Timme, Maximilian, Jens Borkert, Nina Nagelmann, Adam Streeter, André Karch, and Andreas Schmeling. "Age-dependent decrease in dental pulp cavity volume as a feature for age assessment: a comparative in vitro study using 9.4-T UTE-MRI and CBCT 3D imaging." International Journal of Legal Medicine 135, no. 4 (2021): 1599–609. http://dx.doi.org/10.1007/s00414-021-02603-1.

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AbstractEvaluation of secondary dentin formation is generally suitable for age assessment. We investigated the potential of modern magnetic resonance imaging (MRI) technology to visualize the dental pulp in direct comparison with cone beam computed tomography (CBCT). To this end, we examined 32 extracted human teeth (teeth 11–48 [FDI]) using 9.4-T ultrashort echo time (UTE)-MRI and CBCT (methods). 3D reconstruction was performed via both manual and semi-automatic segmentation (settings) for both methods in two runs by one examiner. Nine teeth were also examined by a second examiner. We evaluated the agreement between examiners, scan methods, and settings. CBCT was able to determine the pulp volume for all teeth. This was not possible for two teeth on MRI due to MRI artifacts. The mean pulp volume estimated by CBCT was consistently higher (~ 43%) with greater variability. With lower variability in its measurements, evaluation of pulp volume using the MRI method exhibited greater sensitivity to differences between settings (p = 0.016) and between examiners (p = 0.009). The interactions of single-rooted teeth and multi-rooted teeth and method or setting were not found to be significant. For examiner agreement, the mean pulp volumes were similar with overlapping measurements (ICC > 0.995). Suitable for use in age assessment is 9.4-T UTE-MRI with good reliability and lower variation than CBCT. For MRI, manual segmentation is necessary due to a more detailed representation of the interior of the pulp cavity. Since determination of pulp volume is expected to be systematically larger using CBCT, method-specific reference values are indispensable for practical age assessment procedures. The results should be verified under in vivo conditions in the future.
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6

Sari, Nikita Ika, Diatri Nari Ratih, and Pribadi Santosa. "Management of Ellis Class III Fracture in Two Maxillary Incisivus." Journal of Biomimetics, Biomaterials and Biomedical Engineering 48 (November 2020): 29–33. http://dx.doi.org/10.4028/www.scientific.net/jbbbe.48.29.

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Introduction. Trauma injury in the oral cavity due to collisions can cause tooth fracture. Ellis class III fracture is a fracture of the crown with an open pulp. Teeth with exposed pulp will cause irritation of the pulp resulting in pulp inflammation (pulpitis). One visit pulpectomy and jacket crown with posts were carried out to achieve optimal dental functions. Case report. A 27-year-old male patient came to Dental Hospital of Universitas Gadjah Mada complained about his broken front teeth due to accident three days earlier. The crown of the maxillary right lateral incisor and the maxillary left central incisor with open pulp was seen. The tooth 11 was avulsion, which lost at the location of accident. The diagnosis of 12 and 21 were Ellis class III fracture. Initially an infiltration anaesthesia was done, followed by one visit root canal treatment (RCT). On the second visit, the teeth were asymptomatic, hence the jacket crown preparation and fiber post insertion were performed. On the third visit, a porcelain jacket crown was cemented into teeth 12 and 21. A week later the patient came for recall and no complaints were noted. Conclusion. One visit pulpectomy followed by restoration with porcelain crown and fiber posts can be undertaken to achieve the optimal functions of teeth, namely aesthetic, mastication, phonetics, and protection of supporting tissues function.
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7

Inokuchi, Mikiko, Jouji Nomura, Yoshihiko Mtsumura, Motoko Sekida, and Toshiro Tagawa. "Sotos syndrome with enamel hypoplasia: a case report." Journal of Clinical Pediatric Dentistry 25, no. 4 (2001): 313–16. http://dx.doi.org/10.17796/jcpd.25.4.n52851t26173843u.

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A rare case of Sotos syndrome with enamel hypoplasia is described. Dental abnormalities include enamel hypoplasia, expansion of the pulp cavity, high arched palate, and absence of the bilateral premolar teeth of the mandible.
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8

Al Bukhary, R., R. Wassell, S. Sidhu, O. Al Naimi, and J. Meechan. "The Local Anaesthetic Effect of a Dental Laser Prior to Cavity Preparation: A Pilot Volunteer Study." Operative Dentistry 40, no. 2 (2015): 129–33. http://dx.doi.org/10.2341/13-101-c.

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SUMMARY Objectives It has been suggested that laser preconditioning can produce dental anaesthesia. This study aimed to assess the response of the dental pulp to laser preconditioning. Methods The effects of laser preconditioning, sham laser (negative control), and composite curing light (positive control) on the response of the dental pulp to electric pulp testing was investigated in this double-blind crossover trial with six volunteers. The Er,Cr:YSGG laser or curing light was shone on a premolar tooth in a sweeping motion for 30 seconds (in the sham treatment, the laser was not activated) in blindfolded volunteers subjected to a consistent aural stimulus. Treatment method at each visit was randomized and performed by a researcher not involved in pulp testing. Teeth were pulp tested twice initially by another member of the research team to get baseline readings, immediately following the treatment, and thereafter every two minutes for 10 minutes. Results were analyzed using analysis of variance and an independent-sample t-test. Results There were no significant differences in pulpal response between treatments (p>0.05). Conclusion Laser preconditioning did not affect pulpal response as measured by an electronic pulp tester. Laser preconditioning did not result in any pain or noticeable symptoms for both teeth and soft tissues.
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9

Timme, Maximilian, Jens Borkert, Nina Nagelmann, and Andreas Schmeling. "Evaluation of secondary dentin formation for forensic age assessment by means of semi-automatic segmented ultrahigh field 9.4 T UTE MRI datasets." International Journal of Legal Medicine 134, no. 6 (2020): 2283–88. http://dx.doi.org/10.1007/s00414-020-02425-7.

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Abstract Dental methods are an important element of forensic age assessment of living persons. After the development of all the teeth, including third molars, is completed, degenerative characteristics can be used to assess age. The radiologically detectable reduction of the dental pulp cavity has been described as such a feature. We investigated the suitability of ultrahigh field 9.4 T ultrashort time echo (UTE) magnetic resonance imaging (MRI) for the evaluation of pulp cavity volume in relation to the total tooth volume in 4 extracted human teeth. The volume calculations were performed after semi-automatic segmentation by software AMIRA using the different intensities of the structures in the MRI dataset. The automatically selected intensity range was adjusted manually to the structures. The visual distinction of pulp and tooth structure was possible in all cases with in-plane resolution < 70 μm. Ratios of tooth/pulp volume were calculated, which could be suitable for age estimation procedures. Intensity shifts within the pulp were not always correctly assigned by the software in the course of segmentation. 9.4 T UTE-MRI technology is a forward-looking, radiation-free procedure that allows the volume of the dental pulp to be determined at high spatial resolution and is thus potentially a valuable instrument for the age assessment of living persons.
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10

Петрикас, Олег, Oleg Petrikas, Арнольд Петрикас, et al. "PULPOTOMIYA AT PROSTHETICS OF TEETH. PILOT RESEARCH." Actual problems in dentistry 14, no. 2 (2018): 48–51. http://dx.doi.org/10.18481/2077-7566-2018-14-2-48-51.

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The subject. Significant preparation of the teeth for metal-ceramic or ceramic crowns in most cases is accompanied by a preliminary devitalization of the teeth, which shortens the period of their survival.
 The problem of ensuring the vitality of supporting teeth can be solved by amputating only the coronal pulp, which inevitably overheats during preparation, while retaining the root pulp.
 The skeptical attitude of dentists to vital pulpotomy may be explained by the often unsuccessful (ineffective) use of this method for teeth with already affected pulp. The hope for success in this study is based on the choice of intact teeth for vital pulpotomy of subsequent prosthetics.
 Urgency. The amputation method of endodontic dentistry is not used for prosthetic purposes. This can be explained by its low efficiency when using teeth with inflamed pulp.
 The aim of the work is to confirm the effectiveness of vital pulpotomy in a typical dental situation and subsequent prosthetics.
 Methodology. Clinical observation of the patient before and during 1 year after treatment, with the use of clinical and modern paraclinical methods of examination. Diagnosed. Treatment is planned: removable denture on the upper jaw with a preliminary shortening of the tooth 46 and subsequent use of a metal-ceramic crown. Significant sosotoyanie hard tissues of the tooth 46 and the subsequent preparation for artificial crowns required endodontic-specific training. It was supposed to preserve the root pulp (vital pulpotomy).
 As a result, there were no complaints at the control examination 1 year after pulpotomy. The pain threshold from the electrode was 11 µa.
 Summary. The vital pulpotomy of the tooth with intact pulp showed the possibility of its effective prosthetics with an artificial crown while maintaining the viability of the root pulp. The technique of introduction of the electrode into the cavity of the tooth allowed to conduct direct measurements of the pain threshold of the root pulp in the dynamics of (application for invention No. 2017115518/17(026915) from 02.05.2017).
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11

Kaskova, L. F., O. E. Berezhna, N. V. Yanko, and K. A. Lazareva. "CLINICAL CASE OF DENTINE DYSPLASIA TYPE I." Ukrainian Dental Almanac, no. 2 (June 29, 2021): 107–11. http://dx.doi.org/10.31718/2409-0255.2.2021.19.

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Developmental dental hard tissues disorders are rarely diagnosed both in children and adults, and their treatment is a problem for dentist. Despite the fact that molecular genetics studies allow to discriminate some dentine disorders, pathogenesis of radicular dentin dysplasia is still obscure. Type I dentin dysplasia is characterized by normal or slightly coloured crowns with no roots or only rudimentary roots, reduced pulp space in permanent teeth and incomplete or total obliteration of the pulp chambers, and periapical radiolucent areas or cysts which might result in premature loss of tooth. Fortunately, teeth demonstrate higher resistance to caries than normal teeth do.
 Dentin dysplasia type II is characterized by yellow, brown, grey, translucent primary teeth with complete pulpal obliteration. Permanent teeth are normal or might be slightly coloured. Roots are normal in size, but pulp chamber has pulp stones. A third type of dentine dysplasia or focal odontoblastic dysplasia has radiographic aspects of the other two types of disease.
 Also dentine dysplasia type I has subtypes Ia, Ib, Ic and Id. Dentine dysplasia Ia is characterized by complete obliteration of the pulp, disorder of root development, and many periapical radiolucent areas. A single small horizontally oriented and half-moon shaped pulp is presented in case of dentine dysplasia Ib, roots have a few millimetres length and frequent periapical radiolucencies. Dentine dysplasia Ic is characterized by the presence of two horizontal or vertical semicircle-shaped pulpal remnants which surround dentine in pulp chamber. Also teeth have shortened roots and variable periapical radiolucencies. Dentine dysplasia Id is characterized by the distinct pulp chambers with pulp stones in the coronal third of the root canal; periapical radiolucencies are possible as well. Aim of this study was to analyse clinical case of dentine dysplasia in 8,5-year-old boy.
 A 8,5-year old boy patient with parents came to a dental office due to caries cavity in tooth 62. Shape of tooth crowns was not changed and teeth with no signs of mobility; white lines and spots on teeth are were detected. Based on age, the decision of orthopantomogram was made to evaluate status of primary and permanent teeth. The image showed almost obliterated pulp chamber in both erupted and developing teeth and considerably shortened, blunted and malformed roots without a visible lumen of root canals in primary teeth. Periapical lesions were found about roots of primary molars and follicles of teeth 12 and 14 without caries lesions.
 Patient's medical history revealed no disturbance in general health. Clinical examination of the patient's mother did not show dental hard tissues disorders, but radiograph examination revealed such disorder in the orthopantomogram. Thus, history, clinical and radiographic findings revealed this case as dentine dysplasia type I subtype 1c inherited from mother.
 Oral rehabilitation of patients with dentine dysplasia type I require effective prevention and a complex approach. Thus, meticulous oral hygiene measures and dietary instructions, regular check-ups twice a year were established for this patient; orthodontic consultation was recommended. Varnish «Ftoplen-LC» was applied on the permanent teeth 3 times. To prevent early exfoliation, plan of preventive measures was developed included application of fluoride varnish twice a year and fissure sealing in the premolars in 9-10 years and in the second premolars in 12-13 years.
 Treatment of dentine dysplasia I type that depends on patient age and severity of disease may include also filling of carious teeth, orthodontic, surgical and orthodontic treatment. In this regard, dentist has to know developmental dental hard tissues disorders and select measures to prolong the retention of affected teeth maintaining them as long as possible.
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12

Lima, Thiago Farias Rocha, Juliana Yuri Nagata, Francisco José de Souza-Filho, and Adriana de Jesus Soares. "Post-traumatic Complications of Severe Luxations and replanted Teeth." Journal of Contemporary Dental Practice 16, no. 1 (2015): 13–19. http://dx.doi.org/10.5005/jp-journals-10024-1628.

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ABSTRACT Aim The aim of this study was to evaluate the main posttraumatic complications of severe luxation and replanted teeth using clinical and radiographic analyses. Materials and methods Eighty-three patients aged between 7 and 55 years old presenting 180 traumatized teeth that suffered extrusive luxation (n = 67), lateral luxation (n = 69), intrusive luxation (n = 10) and tooth avulsion (n = 34) followed by replantation were evaluated. The follow-up period was 24 months. The complications examined were: pulp canal obliteration, pulp necrosis and root resorption (inflammatory and replacement). Furthermore, the relationship between time elapsed before receiving dental attendance and development of inflammatory resorption was observed. Results Pulp necrosis was the main complication, occurring in 147 teeth (82.7%). All of the teeth that suffered intrusive luxation and tooth avulsion were diagnosed with pulp necrosis, with significant difference in comparison with another traumas (p < 0.001/Fisher's exact test). Inflammatory root resorption was observed in 20.5% of the cases and replacement resorption was more related to tooth replantation (94.1%), showing significant prevalence among tooth luxations (p < 0.001/Fisher's exact test). In addition, it was noted that patients who seek treatment 9 weeks after the trauma episode presented 10 times more chance of developing inflammatory resorption when compared with patients who seek treatment soon after dental trauma (Odds ratio test). Conclusion It may be concluded that pulp necrosis was the main post-traumatic complication observed in traumatized teeth and that delay in seeking treatment may damage the prognosis of severe luxation and replanted teeth. Clinical significance Clinical studies describing the main complications that may affect traumatized teeth present great relevance to make the population aware of the importance of seeking immediate treatment and to alert the professional to the need for follow-up. When traumatic injuries are diagnosed and treated early, post-traumatic complications may be controlled, allowing conservation of the tooth in oral cavity. How to cite this article Lima TFR, Nagata JY, de Souza- Filho FJ, de Jesus Soares A. Post-traumatic Complications of Severe Luxations and replanted Teeth. J Contemp Dent Pract 2015;16(1):13-19.
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13

DZIECH, JUSTYNA, and TOMASZ PIASECKI. "Anatomical and histological structure of the cavy’s teeth as a basis for diagnosis of dental diseases." Medycyna Weterynaryjna 77, no. 01 (2021): 6500–2021. http://dx.doi.org/10.21521/mw.6500.

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The cavy’s dentition can be shortly described as diphyodont, heterodont, and hypselodont. Histologically, each tooth consists of enamel formed of 4 layers of cells, which together form an apical bud, dentin formed by odontoblasts, and dental cementum. The facies lingualis of incisors is covered with classical acellular cementum, whereas a few circular islands of cementum pearls occur on facies labialis. There are 3 types of cementum in cheek teeth: acellular cementum, cementum pearls, and cartilage-like cementum. Constant tooth growth is ensured by an open pulp cavity within the apex. Periodontal ligaments that are part of the desmodontium are responsible for anchoring teeth in the alveolus.
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14

Sehrawat, Jagmahender Singh, and Monika Singh. "Dental health status of skeletal remains excavated from an abandoned well at Ajnala, India: a bioarchaeological study." Anthropological Review 81, no. 3 (2018): 307–24. http://dx.doi.org/10.2478/anre-2018-0025.

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Abstract Present study was conducted to assess dental health status of the mid-19th century skeletal remains excavated from an abandoned ancient well and to scrutinize the demographic affiliations of the remains. Thousands of bones, teeth and contextual items were excavated non-scientifically from an abandoned well situated underneath a religious structure at Ajnala (Amritsar, India). Four thousands four hundred and seventy five teeth of each type were examined for presence of dental pathologies of caries, wear and linear enamel hypoplasia (LEH). The frequency distribution of each pathological state was calculated for each tooth type using international dental standards available in the literature. The overall dental status was characteristic of young individuals having low prevalence of dental caries and enamel hypoplasiac lesions. Very few teeth had seriously exposed dentine and pulp cavity; indicating consumption of some cariogenic food items. The maxillary and posterior teeth were more affected with dental caries than the mandibular and anterior teeth. LEH defects were found more commonly distributed in the lower canines (44.9%) and upper incisors (34.8%) than their corresponding counterparts and statistically significant differences were noticed in LEH prevalence between anterior and, posterior dentition. The overall percentage of LEH has been found as 15.8%; with 15.4% maxillary and 16.2% mandibular teeth being affected with LEH. The paleodontogical examinations, contextual items and the preliminary molecular findings supported the written versions that victims of Ajnala skeletal remains had sound dental health status and probably belonged to adult males who were killed in 1857 as per written versions.
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15

Anand, Pradeep S., and K. Nandakumar. "Management of Periodontitis Associated with Endodontically Involved Teeth: A Case Series." Journal of Contemporary Dental Practice 6, no. 2 (2005): 118–29. http://dx.doi.org/10.5005/jcdp-6-2-118.

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Abstract The pulp and the periodontal attachment are the two components that enable a tooth to function in the oral cavity. Lesions of the periodontal ligament and adjacent alveolar bone may originate from infections of the periodontium or tissues of the dental pulp. The simultaneous existence of pulpal problems and inflammatory periodontal disease can complicate diagnosis and treatment planning. The function of the tooth is severely compromised when either one of these is involved in the disease process. Treatment of disease conditions involving both of these structures can be challenging and frequently requires combining both endodontic and periodontal treatment procedures. This article presents cases of periodontitis associated with endodontic lesions managed by both endodontic and periodontal therapy. Citation Anand PS, Nandakumar K. Management of Periodontitis Associated with Endodontically Involved Teeth: A Case Series. J Contemp Dent Pract 2005 May;(6)2:118-129.
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16

Petrovic, Violeta, Jovana Stasic, Vojislav Komlenic, Tatjana Savic-Stankovic, Marina Latkovic, and Vesna Miletic. "Temperature changes in the pulp chamber induced by polymerization of resin-based dental restoratives following simulated direct pulp capping." Chemical Industry 73, no. 4 (2019): 239–48. http://dx.doi.org/10.2298/hemind190504020p.

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The objective of this study was to measure temperature changes in the pulp chamber induced by polymerization of resin-based dental restoratives following a simulated procedure of direct pulp capping. Class I cavities with a microperforation at the pulp horn were prepared in extracted human molar teeth. The complete procedure of direct pulp capping and cavity restoration was performed with the root part of extracted teeth fixed in a water bath at 37 ?C. Mineral trioxide aggregate, bioactive dentin substitute or calcium-hydroxide paste were used as pulp capping materials. Cavities were restored with a light-cured or chemically-cured resin-modified glass ionomer, universal adhesive and a bulk-fill composite, cured with a high-intensity LED unit. Pulp capping materials caused a slight temperature decrease. Lower temperature increase was recorded during light-curing of the glass ionomer liner after direct capping with mineral trioxide aggregate and calcium-hydroxide than that recorded for the bioactive dentin substitute. Adhesive light-curing increased temperature in all groups with higher mean temperatures in groups with chemically-cured as compared to those for the light-cured glass ionomer liner. Direct pulp capping with mineral trioxide aggregate or calcium-hydroxide followed by the light-cured resin-modified glass ionomer liner and a bonded bulk-fill composite restoration induced temperature changes below the potentially adverse threshold of 42.5?C.
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17

Ivashchenko, Victoria А., Anatoly А. Adamchik, Armenak V. Аrutyunov, Sergey I. Risovanny, Alexander N. Sidorenko, and Oleg V. Tsymbalov. "Morphological Changes in the Dental Pulp of Experimental Animals in the Treatment of Acute Partial Pulpitis using Modern Materials." Kuban Scientific Medical Bulletin 26, no. 5 (2019): 29–41. http://dx.doi.org/10.25207/1608-6228-2019-26-5-29-41.

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Aim. To study the morphological changes in the tooth pulp of experimental animals when modelling acute partial pulpitis using calcium silicate modified with light-cured resin and calcium hydroxide.Materials and methods. An experimental model of acute partial pulpitis was applied including the formation of a cavity on the lingual surface of the tooth, which corresponded to advanced caries in terms of depth, followed by a slight perforation up to the tooth pulp with a sharp probe. This model of acute partial pulpitis was reproduced for 32 teeth in four sexually mature rams. The teeth were extracted together with the surrounding sockets 15, 30 and 90 days after the modelling of pulpitis, followed by their fi xation in 10% solution of neutral formalin. Decalcification was performed in 25% solution of Trilon B. The material was poured into celloidin; histological sections were stained with hematoxylin and eosin according to Masson’s trichrome procedure.Results. The histological examination of pulp from the main group (calcium silicate modified with light-cured resin) revealed the formation of a biological barrier from secondary dentin in the area of the cavity and perforation up to the pulp chamber on the 90th day after the experiment started. The tooth pulp revealed no signs of an inflammatory process. On the 90th day after the experiment started, no pronounced morphological changes in the main substance and dentinal tubules in the area of the cavity walls were noted in the coronal pulp of experimental animals from the comparison group (calcium hydroxide). A focal chronic inflammatory process was noted in the tooth pulp.Conclusion. It was established that the use of calcium silicate modified with light-cured resin in the experimentally created model of acute partial pulpitis was accompanied by the formation of a biological barrier and a well-developed zone of reparative dentin in the area of the cavity and perforation up to the pulp chamber, as compared to the group where calcium hydroxide was used. Thus, the study results confirmed the efficay of using calcium silicate modified with light-cured resin within the biological method for treating acute partial pulpitis, which shortens the functional recovery of tooth pulp.
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18

Fageeh, Hytham N. "Preliminary Evaluation of Proliferation, Wound Healing Properties, Osteogenic and Chondrogenic Potential of Dental Pulp Stem Cells Obtained from Healthy and Periodontitis Affected Teeth." Cells 10, no. 8 (2021): 2118. http://dx.doi.org/10.3390/cells10082118.

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Background: Dental pulp tissue within the central cavity of the tooth is composed of dental pulp stem cells (DPSC). These mesenchymal stem cells have good proliferative as well as differentiation potential. DPSC has been isolated even from teeth with inflamed pulps and is found to retain their proliferative and differentiation potential. Little research is available about the viability and differentiation potential of DPSC obtained from teeth with periodontitis. In the present study, the aim was to compare the morphological features, stem cell marker (MSC) expression, proliferation rate, migratory and wound healing properties, osteogenic and chondrogenic differentiation potential of DPSCs obtained from periodontally healthy teeth (hDPSCs) and periodontitis affected teeth (pDPSCs). Methods: Dental pulp tissue was obtained from periodontally healthy volunteers (n = 3) and patients with periodontitis undergoing extraction of mobile teeth (n = 3). DPSC were isolated using the explant technique and cultured. All the experiments were performed at early passage (Passage 2), late passage (Passage 6) and after cryopreservation. Morphological features of the hDPSCs and pDPSCs were ascertained using microscopy. The expression of cell surface stem cell markers was assessed by the flow cytometry method. The proliferation and growth rate of the cells were assayed by plotting a growth curve from 0–13 days of culture. The migratory characteristics were assessed by wound scratch assay. Osteogenic and chondrogenic differentiation of the cells was assessed using standard protocols with and without induction. Results: DPSCs were successfully obtained from periodontally healthy teeth (hDPSC) and periodontitis-affected teeth (pDPSCs). The data suggests that there were no morphological differences observed in early passage cells between the two cohorts. Cryopreservation did change the morphology of pDSPCs. There was no significant difference in the positive expression of mesenchymal markers CD73, CD90 and CD105 in early passage cells. However, serial passaging and cryopreservation affected the marker expression in pDPSCs. A faint expression of hematopoietic stem cell markers CD34, CD45 and MHC class II antigen HLA-DR was observed in both the cell types. The expression of HLA-DR is upregulated in pDPSCs compared to hDPSC. A significantly slower growth rate and slower wound healing properties was observed in pDPSCs compared to hDPSC. In late passage and after cryopreservation, the migratory ability of pDPSCs was found to be increased drastically. There was no significant difference in osteogenic potential between the two cell types. However, the chondrogenic potential of pDPSCs was significantly lower compared to hDPSc. Yet, pDPSCs showed enhanced osteogenesis and chondrogenesis at late passage as well as after cryopreservation. Conclusion: The results of this novel study shed light on the isolation of viable DPSC from periodontitis-affected teeth. These cells exhibit a slower growth rate and migratory characteristics compared to their healthy counterparts. There was no difference in osteogenic potential but a reduction in chondrogenic potential was seen in pDPSCs compared to hDPSC. The findings reveal that DPSC from periodontitis-affected teeth presents an easy and viable option for regenerative medicine application. Some additional nutritive factors and protocols may be required to attain better regenerative benefits while using pDPSCs.
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Hegde, Shruthi, Kamath Shanti, Vidya Ajila, Subhas Babu, and Supriya Bhat. "Dens evaginatus and dens invaginatus: A report of two cases." Acta stomatologica Naissi 36, no. 82 (2020): 2091–97. http://dx.doi.org/10.5937/asn2082091h.

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Introduction: Developmental disturbances of teeth at the stage of morpho-differentiation have been related to abnormalities associated with changes in the tooth shape and size. Dens evaginatus and dens invaginatus are the developmental variations of the human dentition. Dens evaginatus, a rare anomaly characterized by the presence of a tubercle on the occlusal surface of teeth is seen to occur due to abnormal proliferation and folding of the inner enamel epithelium and part of the dental papilla into the stellate reticulum of the enamel organ; whereas, dens invaginatus is seen to occur due to infolding of the enamel and dentine into the pulp cavity and sometimes extending to the root apex. Case report: We report cases with bilateral dens evaginatus in mandibular second premolars and dens invaginatus in maxillary lateral incisor. Conclusion: Such developmental anomalies of teeth deserve clinical importance, as high chances of early pulpal pathosis.
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Manchorova-Veleva, Neshka А. "Three-dimensional analysis of cavity wall deformation after composite restoration of masticatory teeth." Folia Medica 53, no. 4 (2011): 53–59. http://dx.doi.org/10.2478/v10153-011-0068-8.

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ABSTRACT AIM: The aim of the present work was to study the size of cavity wall deformation in eight class I and II defects after composite restoration. MATERIALS AND METHODS: 1. Creating a geometric model - data on the size of the left maxillary second premolar were obtained from a routine craniofacial scanning of a 20-yearold patient with a 2,5 Dental CT scanner (General Electric), with high resolution and 0.625mm-thin slices. The contour of each of the 33 cross-sections of tooth 25 was delineated using graphics software (CorelDraw 7.0) and transferred to a specialized product for engineering design (SolidWorks Offi ce Premium 2010, SolidWorks Corp. USA). The pulp cavity and periodontal ligament were created in the same manner and were integrated in the premolar body; 2. Generation of a fi nite element method - the geometric model was exported to specialized software for analysis by the fi nite element method - COSMOSWorks 2010, which automatically builds a 3D fi nite elements mesh. Based on the generated model, eight additional models of class I and II cavities with different geometries, adhesive layer and nanofi lled composite restorations were constructed. The polymerization shrinkage was modelled by thermal deformation, with a negative temperature difference (cooling), corresponding to the actual volume shrinkage of the composite materials by 2.1%. RESULTS: In models A and B, the maximum cavity wall displacement was small - 0.014 mm and 0.015 mm, respectively. In models A1, B1, C1 and C, the displacement was at the expense of large deformation of the dental tissues. The maximum cavity wall displacements were 0.020 mm, 0.026 mm, 0.020 mm, 0.035 mm, respectively. The least cavity wall displacement was in models A2 and B2 with 0.008 mm and 0.017 mm, respectively. CONCLUSIONS: The least displacement resulting from cavity wall deformation is found in patient-friendly class I and II preparations. Preservation of the dental tissues reduces the risk of mechanical pressure on the dentinal lymph and the likelihood of post-operative sensitivity.
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COSTA, Mirella Aline Batista da, Juliana Raposo Souto MAIOR, Renata Pedrosa GUIMARÃES, Daene Patrícia Tenório Salvador da COSTA, Paulo Fonseca MENEZES FILHO, and Claudio Heliomar Vicente da SILVA. "Restorations with Bulk Fill restorative system: case report." RGO - Revista Gaúcha de Odontologia 66, no. 4 (2018): 391–97. http://dx.doi.org/10.1590/1981-8637201800040000143505.

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ABSTRACT Bulk Fill Composite resins are restorative materials that present low tension and polymerization shrinkage, allowing them to be inserted in a single increment of up to 4 mm of thickness, in a restorative cavity. This paper aims to report a clinical case of restorations done on posterior teeth with Bulk Fill restorative system, using selective acid-etching with self-etch adhesive system. Patient, male, 24 years old, presented a carious lesion on the occlusal surface of dental element 37 and an occlusal unsatisfactory amalgam restoration, on element 36. A restorative treatment with Universal Bond 3M / ESPE and bulk fill composite resin (3M / ESPE) was done. According to the case described, it was possible to observe that the materials and techniques used restored the form and function of the teeth involved, preserving pulp vitality with a satisfactory aesthetic result. The bulk fill resins employed offer practicality and decrease clinical time, with satisfactory clinical applicability in the aesthetic and functional rehabilitation of posterior teeth.
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Kadota, Tamami, Masakazu Hamada, Ryota Nomura, et al. "Distribution of Helicobacter pylori and Periodontopathic Bacterial Species in the Oral Cavity." Biomedicines 8, no. 6 (2020): 161. http://dx.doi.org/10.3390/biomedicines8060161.

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The oral cavity may serve as a reservoir of Helicobacter pylori. However, the factors required for H. pylori colonization are unknown. Here, we analyzed the relationship between the presence of H. pylori in the oral cavity and that of major periodontopathic bacterial species. Nested PCR was performed to detect H. pylori and these bacterial species in specimens of saliva, dental plaque, and dental pulp of 39 subjects. H. pylori was detected in seven dental plaque samples (17.9%), two saliva specimens (5.1%), and one dental pulp (2.6%) specimen. The periodontal pockets around the teeth, from which dental plaque specimens were collected, were significantly deeper in H. pylori-positive than H. pylori-negative subjects (p < 0.05). Furthermore, Porphyromonas gingivalis, a major periodontopathic pathogen, was detected at a significantly higher frequency in H. pylori-positive than in H. pylori-negative dental plaque specimens (p < 0.05). The distribution of genes encoding fimbriae (fimA), involved in the periodontal pathogenicity of P. gingivalis, differed between H. pylori-positive and H. pylori-negative subjects. We conclude that H. pylori can be present in the oral cavity along with specific periodontopathic bacterial species, although its interaction with these bacteria is not clear.
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Balan, Gülşah, Harry Huiz Peteers, and Serap Akyüz. "The assistance of Er,Cr:YSGG laser in pulp injury related to anterior teeth trauma." Dental Journal (Majalah Kedokteran Gigi) 52, no. 2 (2019): 57. http://dx.doi.org/10.20473/j.djmkg.v52.i2.p57-60.

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Background: Erbium chromium or erbium lasers constitute preferred instruments for the preparation of that section of tooth adjacent to the pulp chamber rather than high-speed drills, especially in cases of dental injury. Their advantages can support modified operations in achieving optimum recovery and avoiding complications related to the healing process. Purpose: The aim of the study was to describe another modality of the assistance of erbium, chromium:yattrium-scandium-gallium-garnet (Er,Cr:YSGG) laser in traumarelated pulp injury affecting the anterior teeth. Case(s): The results of laser treatment applied to four pulp injuries of three children were analyzed in this case report. Case Management: Er,Cr:YSGG laser-assisted cavity preparation was performed without resort to a local anesthetic, the cavities being sealed with mineral trioxide aggregate (MTA). None of the cases demonstrated symptomatic or peri-radicular pathology during clinical or radiographic examinations. Conclusion: The results contained in this case report support the application in pediatric dentistry of a Er,Cr:YSGG laser to pulpotomy cavities as part of the treatment of traumatic pulpal injuries to permanent incisors.
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D’amico, Yohanna Campos, Ulisses Xavier Da Silva Neto, Vânia Portela Ditzel Westphalen, Everdan Carneiro, and Alexandre Kowalczuck. "Fracture strength of teeth with access cavity preparation with operating microscope or on buccal surfaces." Brazilian Dental Science 22, no. 1 (2019): Process. http://dx.doi.org/10.14295/bds.2019.v22i1.1655.

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Objective: The present study assessed the fracture strength of teeth subjected to endodontic access cavity preparation on buccal surfaces, or with the aid of operating microscopy when compared to the conventional technique. Material and methods: Sixty mandibular incisors were split into four groups (n=15): conventional access cavity preparation (CCP); conservative (C); buccal surface (BS); and control. The canals were prepared and filled and the cavities were restored. A static compressive strength test was conducted until crown fracture. The force data were compiled and assessed statistically. Kolmogorov-Smirnov and Shapiro-Wilk tests were performed to assess normality, Levene’s test to assess variance homogeneity, the one-way ANOVA to compare fracture strength in the assessed groups. Tukey’s HSD test was used to determine whether the differences in the means were significant between the groups.Results: The experimental groups did not show any statistically significant differences in mean fracture strength (CCP = 585.65 N±107.64 N)(BS = 530.52 N±129.35 N) (C = 517.83 N±114.68 N). Conclusion: Therefore, the selection of surface or size of access cavity proposed did not influence the fracture strength of mandibular incisors when compared to conventional cavity preparation. KeywordsDental pulp cavity; Dental stress analysis; Root canal therapy.
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Duan, Wei, Yufei Chen, Qi Zhang, Xiang Lin, and Xiaoyu Yang. "Refined tooth and pulp segmentation using U-Net in CBCT image." Dentomaxillofacial Radiology 50, no. 6 (2021): 20200251. http://dx.doi.org/10.1259/dmfr.20200251.

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Objectives The aim of this study was extracting any single tooth from a CBCT scan and performing tooth and pulp cavity segmentation to visualize and to have knowledge of internal anatomy relationships before undertaking endodontic therapy. Methods: We propose a two-phase deep learning solution for accurate tooth and pulp cavity segmentation. First, the single tooth bounding box is extracted automatically for both single-rooted tooth (ST) and multirooted tooth (MT). It is achieved by using the Region Proposal Network (RPN) with Feature Pyramid Network (FPN) method from the perspective of panorama. Second, U-Net model is iteratively performed for refined tooth and pulp segmentation against two types of tooth ST and MT, respectively. In light of rough data and annotation problems for dental pulp, we design a loss function with a smoothness penalty in the network. Furthermore, the multi-view data enhancement is proposed to solve the small data challenge and morphology structural problems. Results: The experimental results show that the proposed method can obtain an average dice 95.7% for ST, 96.2% for MT and 88.6% for pulp of ST, 87.6% for pulp of MT. Conclusions This study proposed a two-phase deep learning solution for fast and accurately extracting any single tooth from a CBCT scan and performing accurate tooth and pulp cavity segmentation. The 3D reconstruction results can completely show the morphology of teeth and pulps, it also provides valuable data for further research and clinical practice.
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Sinescu, Cosmin, Meda Lavinia Negrutiu, Adrian Bradu, Virgil-Florin Duma, and Adrian Gh Podoleanu. "Noninvasive Quantitative Evaluation of the Dentin Layer during Dental Procedures Using Optical Coherence Tomography." Computational and Mathematical Methods in Medicine 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/709076.

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A routine cavity preparation of a tooth may lead to opening the pulp chamber. The present study evaluates quantitatively, in real time, for the first time to the best of our knowledge, the drilled cavities during dental procedures. An established noninvasive imaging technique, Optical Coherence Tomography (OCT), is used. The main scope is to prevent accidental openings of the dental pulp chamber. Six teeth with dental cavities have been used in thisex vivostudy. The real time assessment of the distances between the bottom of the drilled cavities and the top of the pulp chamber was performed using an own assembled OCT system. The evaluation of the remaining dentin thickness (RDT) allowed for the positioning of the drilling tools in the cavities in relation to the pulp horns. Estimations of the safe and of the critical RDT were made; for the latter, the opening of the pulp chamber becomes unavoidable. Also, by following the fractures that can occur when the extent of the decay is too large, the dentist can decide upon the right therapy to follow, endodontic or conventional filling. The study demonstrates the usefulness of OCT imaging in guiding such evaluations during dental procedures.
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VASCONCELOS, Amanda de Albuquerque, Juliana Tietbohl de Almeida REIS, Bianca Fiorentin MOURA, Daniela Cavalcante GIRÃO, José Carlos Pettorossi IMPARATO, and Sérgio Luiz PINHEIRO. "Microleakage analysis of dental caries lesions sealed with flow resin and compared to microhybrid resin restorations in dentin." RGO - Revista Gaúcha de Odontologia 66, no. 2 (2018): 141–46. http://dx.doi.org/10.1590/1981-863720180002000063343.

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ABSTRACT Objective: To evaluate the sealing of cavities of dentinal occlusal caries lesions, reproduced in vitro, with flow resin compared to cavity restorations presenting healthy dentin using microhybrid composite resin. Methods: The sample consisted of 27 healthy deciduous molars where cavities of approximately 2 mm in the fossa region were performed and occlusal cleft of each tooth were sealed, impermeabilization was performed and the sample was randomly divided into 2 groups: group I underwent cariogenic challenge and occlusal sealing with resin flow. The teeth of group II were restored with microhybrid composite resin. The teeth were immersed in 5% methylene blue for 8 hours at 37° C and washed until all the dye was removed from the surface. The teeth were sectioned in the mesio-distal direction. The penetration of the dye was evaluated: 0- no penetration; 1- dye penetration up to 1/3 of the restoration; 2- dye penetration up to 2/3 of the restoration depth; 3 - penetration of dye into the pulp wall. The results were analyzed by the Biostat 4.0 program. Descriptive analysis and the mode among the examiners submitted to the Mann-Whitney test. Results: There was no significant difference in microleakage between restoration performed in healthy dentin with microhybrid composite resin or maintenance of infected dentin in primary teeth sealed with resin flow (p = 0.6035). Conclusion: It was concluded that the marginal infiltration of primary molars sealed with microhybrid composite resin and resin flow was not influenced by the removal -or not -of the carious tissue or the material used.
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Law, A. S., K. R. Baumgardner, S. T. Meller, and G. F. Gebhart. "Localization and Changes in NADPH-Diaphorase Reactivity and Nitric Oxide Synthase Immunoreactivity in Rat Pulp Following Tooth Preparation." Journal of Dental Research 78, no. 10 (1999): 1585–95. http://dx.doi.org/10.1177/00220345990780100301.

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Inflammatory changes in the dental pulp are accompanied by release of a wide variety of chemical mediators. Nitric oxide, an oxidative free radical produced by the enzyme nitric oxide synthase (NOS), has been implicated in multiple inflammatory processes, which makes it a suitable marker for changes which likely occur following tooth pulp insult. Since limited information on nitric oxide in the pulp is available, it is necessary first to examine relative distributions of NOS in uninflamed and inflamed rat pulp. We accomplished this by characterizing regions of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) activity and the distribution of both macrophage NOS (macNOS) and neuronal NOS (nNOS) immunoreactivity in normal and inflamed rat molar pulp at multiple time points. The results showed that: (1) deep cavity preparation on the mesial surface of the molar produced a time-dependent inflammation, with acute inflammation early progressing to chronic, granulomatous inflammation with necrosis later that spread preferentially down the mesial root; (2) control (non-prepared) teeth showed a relatively faint and homogeneous distribution of NADPH-d and macNOS reactivity but no discernible nNOS reactivity; (3) inflamed teeth displayed localized increased intensity of NADPH-d and macNOS reactivity surrounding the inflamed area of pulp, but no increased nNOS activity; (4) pulp vessels supplying the inflamed area showed increased NADPH-d reactivity, but no increased macNOS or nNOS reactivity; and (5) neither NADPH-d, macNOS, nor nNOS reactivity was observed in pulpal nerves. Therefore, nitric oxide may mediate the pulpal inflammatory response through its effects on the paralesional pulp tissue and surrounding endothelial/vascular structures.
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AboulHosn, M., Z. Noujeim, N. Nader, and A. Berberi. "Decompression and Enucleation of a Mandibular Radicular Cyst, Followed by Bone Regeneration and Implant-Supported Dental Restoration." Case Reports in Dentistry 2019 (January 9, 2019): 1–8. http://dx.doi.org/10.1155/2019/9584235.

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Odontogenic cysts are usually treated by enucleation (cystectomy). Limited cysts (less than 5 cm) are usually managed by primary excision (total cystectomy), whereas larger ones (exceeding 5 cm) are often decompressed or marsupialized. Because it consists only of opening a much smaller surgical window, decompression is regarded as a more conservative method of treatment: this method associates the creation of an opening (window) into the cystic cavity with the suturing of a decompressing device (plastic tube or stent) at the periphery of the cyst. Apart from releasing intraluminal pressure in the pathological cavity, this procedure helps the lesion to progressively decrease in volume “with a gradual increase in bone apposition” and preserves pulp vitality and periodontal integrity of the adjacent teeth. We are reporting a case of a mandibular radicular cyst that was treated by decompression, followed by enucleation, bone reconstruction, and restoration with two osseointegrated dental implants. The cystic cavity progressively decreased in volume and increased in bone density.
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30

Gol'din, Pavel, Lena Godlevska, and Maria Ghazali. "Age-Related Changes in the Teeth of Two Bat Species: Dental Wear, Pulp Cavity and Dentine Growth Layers." Acta Chiropterologica 20, no. 2 (2019): 519. http://dx.doi.org/10.3161/15081109acc2018.20.2.022.

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31

Kuzmanović Radman, Irena, Aleksandra Djeri, Adriana Arbutina, and Jelena Milašin. "Microbiological Findings in Deep Caries Lesions." Stomatoloski glasnik Srbije 63, no. 1 (2016): 7–14. http://dx.doi.org/10.1515/sdj-2016-0001.

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SUMMARY Introduction Caries is one of the most significant and widespread oral diseases. It has been confirmed that dental plaque, i.e. microorganisms in it, are the most important factor in the development of dental caries. Caries profunda represents deep carious lesion from where bacterial toxins may affect pulp through dentinal tubules. The aim of this study is to assess the efficacy of indirect pulp capping based on microbiological findings of bacteria present in deep carious lesions before and after the treatment. Material and Methods The clinical study included 29 patients of both genders, aged 16 to 40 and 45 permanent teeth with deep caries lesions. The first microbiological sample was taken after cavity preparation and removal of soft dentin from the bottom of the cavity. The second sample was taken after the removal of temporary filling and calcium hydroxide paste 60 days after the indirect pulp capping treatment. The collected samples were stored in special sterile micro tubes (Eppendorf) and kept at the temperature of −80°C until microbiological analysis was performed. Samples were tested for the presence of the following microorganisms: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Enterococcus faecalis using the multiplex polymerase chain reaction (PCR) method. Results The results showed that prior to the treatment of deep carious lesions the most common species was E. faecalis (80% of samples), followed by A. actinomycetemcomitans (32% of samples), while the least common was P. gingivalis (16% of samples). After the treatment with products based on calcium hydroxide, E. faecalis was registered in 18% of samples, A. actinomycetemcomitans in 16% of samples and P. gingivalis was not registered in any sample. Conclusion The most common bacterial species in teeth with deep caries lesions was E. faecalis, whereas A. actinomycetemcomitans and P. gingivalis were found in lower number of samples.
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Rey Lescure, Manon, Nicola Alberto Valente, Sibylle Chatelain, Chiara Cinquini, and Antonio Barone. "Autotransplantation of Two Immature Third Molars with the Use of L-PRF." Case Reports in Dentistry 2021 (January 2, 2021): 1–8. http://dx.doi.org/10.1155/2021/6672711.

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Tooth autotransplantation is a procedure which provides the extraction of an erupted or impacted tooth and its repositioning to another site in the oral cavity. This Case Report describes a successful case of two autotransplantations of open-apex mandibular third molars in place of the hopeless first mandibular molars with the use of L-PRF in a growing patient. A 15-year-old male patient was referred to the Dental Clinic for the extractions of the two hopeless mandibular first molars. Autotransplantation was considered the best treatment option for both sites 36 and 46, because the presence of two impacted mandibular third molars (38 and 48) with an incomplete root formation. Teeth 36 and 46 were extracted and replaced with teeth 38 and 48. The patient had an uneventful healing. At follow-up visits, the two autotransplanted teeth showed physiologic mobility, absence of inflammation and discomfort, and absence of infection; probing depth values were within normal range, and the vitality tests were positive. After 2 years, the teeth in position 36 and 46 showed absence of infection and mobility, and positive pulp vitality tests and the radiographic examinations exhibited closure of the root apices as well as absence of any periapical radiolucency or root resorption. Tooth autotransplantation is a good treatment option in case of tooth loss offering an alternative to traditional or implant-supported prosthesis especially for growing patients.
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Galler, Kerstin M., Manuel Weber, Yüksel Korkmaz, Matthias Widbiller, and Markus Feuerer. "Inflammatory Response Mechanisms of the Dentine–Pulp Complex and the Periapical Tissues." International Journal of Molecular Sciences 22, no. 3 (2021): 1480. http://dx.doi.org/10.3390/ijms22031480.

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The macroscopic and microscopic anatomy of the oral cavity is complex and unique in the human body. Soft-tissue structures are in close interaction with mineralized bone, but also dentine, cementum and enamel of our teeth. These are exposed to intense mechanical and chemical stress as well as to dense microbiologic colonization. Teeth are susceptible to damage, most commonly to caries, where microorganisms from the oral cavity degrade the mineralized tissues of enamel and dentine and invade the soft connective tissue at the core, the dental pulp. However, the pulp is well-equipped to sense and fend off bacteria and their products and mounts various and intricate defense mechanisms. The front rank is formed by a layer of odontoblasts, which line the pulp chamber towards the dentine. These highly specialized cells not only form mineralized tissue but exert important functions as barrier cells. They recognize pathogens early in the process, secrete antibacterial compounds and neutralize bacterial toxins, initiate the immune response and alert other key players of the host defense. As bacteria get closer to the pulp, additional cell types of the pulp, including fibroblasts, stem and immune cells, but also vascular and neuronal networks, contribute with a variety of distinct defense mechanisms, and inflammatory response mechanisms are critical for tissue homeostasis. Still, without therapeutic intervention, a deep carious lesion may lead to tissue necrosis, which allows bacteria to populate the root canal system and invade the periradicular bone via the apical foramen at the root tip. The periodontal tissues and alveolar bone react to the insult with an inflammatory response, most commonly by the formation of an apical granuloma. Healing can occur after pathogen removal, which is achieved by disinfection and obturation of the pulp space by root canal treatment. This review highlights the various mechanisms of pathogen recognition and defense of dental pulp cells and periradicular tissues, explains the different cell types involved in the immune response and discusses the mechanisms of healing and repair, pointing out the close links between inflammation and regeneration as well as between inflammation and potential malignant transformation.
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Stojsin, Ivana, Ljubomir Petrovic, Igor Stojanac, and Milan Drobac. "Multi-factoriality of dentine hypersensitivity." Medical review 61, no. 7-8 (2008): 359–63. http://dx.doi.org/10.2298/mpns0808359s.

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Introduction. Dentine hypersensitivity has been defined as a sharp, short pain arising from exposed dentin in response to stimuli typically thermal, evaporative tactile, osmoticor, chemical and which cannot be ascribed to any other form of dental defect or pathology. Prevalence. The most affected patients range in age from 20 to 40. The following teeth tend to be most sensitive: cuspids, premolars and incisors, location-concentrated on the facial surface. Morphological bases of dentine hypersensitivity Sensitive teeth have much greater numbers of open tubules per unit area and the average diameter of tubules is almost 2 times greater than tubules in nonsensitive teeth. Mechanisms of dentine hypersensitivity. The most widely accepted theory of how the pain occurs is Brannstroms theory. Etiology Dentine hypersensitivity represents a condition of presumable multifactorial pathology. Two processes are essential for its development: (1) dentin must be exposed through either genetic disturbance, enamel defect (lamellae, tufts and spindles), loss of enamel (erosion, abrasion, attrition, abfraction), gingival recession with rapid loss of cementum and (2) the dentin tubules must be open to both the oral cavity and the pulp. Diagnosis. Diagnostic protocol for this condition consisted of Medical, Dental Dietary, Oral Hygiene History and Intra-oral examinations with air indexing method. Differential Diagnosis: We must take into consideration a number of variables such as: dental caries, cracked tooth, restorative sensitivity, medication sensitivity, bleaching sensitivity and abscessed or non-vital tooth. Conclusion. Dentin hypersensitivity is a problem that bothes many patients. Many conditions share the symptoms of tooth sensitivity so differential diagnosis is essential for suitable treatment or preventive measures.
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Irmawati, Ari Rosita, and Ayulistya Paramita Sutarto. "Prevention of infective endocarditis in dental procedures for tetralogy of fallot patients." Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) 5, no. 2 (2021): 66. http://dx.doi.org/10.32793/jrdi.v5i2.706.

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Objectives: Tetralogy of Fallot (ToF) is a congenital heart defect that is often found in children. Complication that can occur is infective endocarditis caused by bacteria that are often found in the oral cavity. Children with ToF are in a high risk of caries, especially in primary dentition. Preparation of the oral cavity must be done before the cardiac surgery. A proper examination and diagnosis of caries is required in determining the dental treatment to reduce the risk of infective endocarditis.
 Case Report: A boy aged 4 years, weighing 20 kg came to the pediatric dentistry clinic in Surabaya on a referral from Kediri. The patient had ToF with high caries index and will undergo cardiac corrective surgery. Through a brief anamnesis, it was found that this patient's fingernails and lips easily turn blue (cyanosis) if the patient is in an anxious condition. The panoramic photo shows teeth 51, 52, 54, 61, 62, 74, 84 showing radiolucent images from the enamel to the pulp chamber.
 Conclusion: Prophylactic antibiotics should be given to ToF patients before procedures involving the gingiva and pulp. Radiographs are needed to help establish the diagnosis and plan treatment. Proper oral and dental care must be taken to prevent the occurrence of infective endocarditis. Errors in determining the diagnosis can lead to errors in the treatment plan and increase the risk of infective endocarditis.
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Schwendicke, Falk, Soraya Leal, Peter Schlattmann, et al. "Selective carious tissue removal using subjective criteria or polymer bur: study protocol for a randomised controlled trial (SelecCT)." BMJ Open 8, no. 12 (2018): e022952. http://dx.doi.org/10.1136/bmjopen-2018-022952.

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IntroductionSelective (incomplete/partial) carious tissue removal is suitable for treating deep carious lesions in teeth with vital, asymptomatic pulps. In the periphery of a cavity, removal to hard dentin is performed, while in pulpo-proximal areas, leathery or soft dentin is left to avoid pulp exposure. As the decision of what contains ‘soft’ or ‘leathery’ dentin is subjective, using self-limiting burs which help to standardise the hardness of the remaining dentin, has been suggested to increase the reliability of carious tissue removal. The trial compares subjectively measured selective carious tissue removal in deep lesions in primary teeth with objectively measured selective removal with a self-limiting bur (Polybur, Komet).Methods and analysisA community-based single-blind clustered randomised controlled superiority trial nested into a larger evaluation is performed. Recruitment for this trial has been concluded. We have recruited 115 children aged 6–8 years with ≥1 vital primary molar with a deep dentin lesion. The unit of randomisation was the child, with all eligible molars per child treated identically. Treatment was performed in a mobile dental unit. Subjective and objective carious tissue removal was performed at random. Teeth were restored using glass ionomer cement (Equia Forte, GC). Our primary outcome will be the time until complications occur, evaluated via multilevel survival analysis. Secondary outcomes will be the time until extraction is needed, subjective satisfaction of the child with the treatment (measured using a Likert scale) and cost-effectiveness. Re-examination will be performed after 12, 24 and 36 months (the final examination is expected in 2020).Ethics and disseminationThis trial has been approved by the Ethics Committee of the Health Sciences of the University of Brasília (CAAE 51310415.0.0000.0030). Trial results will be published in peer-reviewed journals and presented on conferences.Trial registration numberNCT02754466.
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Kanjevac, Tatjana, Marija Milovanovic, Olivera Milosevic-Djordjevic, Zivoslav Tesic, Mirjana Ivanovic, and Aleksandra Lukic. "Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations." Archives of Biological Sciences 67, no. 2 (2015): 619–30. http://dx.doi.org/10.2298/abs141021022k.

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Stem cells from human exfoliated deciduous teeth (SHED) can be used as a cell-based therapy in regenerative medicine and in immunomodulation. Pulp from human deciduous teeth can be stored as a source of SHED. Glass ionomer cements (GICs) are commonly used in restorative dentistry and in cavity lining. GICs have lower biocompatibility and are cytotoxic for dental pulp cells. In this study, seven commonly used GICs were tested for their cytotoxic effects on SHED, for their potential to arrest mitosis in cells and induce chromosome aberrations, and were compared with the effects of composite. Fuji II, Fuji VIII, Fuji IX, Fuji plus and Vitrebond had significantly higher cytotoxic effects on SHED than composite. Only SHEDs that have been treated with Fuji I, Fuji IX, Fuji plus and composite recovered the potential for proliferation, but no chromosome aberrations were found after treatment with GICs. The cytotoxic effects of GICs on SHEDs were in strong correlation with combined concentrations of released fluoride, aluminum and strontium ions. Fuji I exhibited the lowest activity towards SHEDs; it did not interrupt mitosis and did not induce chromosome aberrations, and was accompanied by the lowest levels of released F, Al and Sr ions. Projekat Ministarstva nauke Republike Srbije, br. ON175069, br. ON175071 i br. ON175103]
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Hidayat, Septian Rahmat, Fahmi Oscandar, Yuti Malinda, et al. "Human age estimation based on pulp volume of canines for chronological age estimation: Preliminary research." Padjadjaran Journal of Dentistry 30, no. 3 (2018): 184. http://dx.doi.org/10.24198/pjd.vol30no3.19302.

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Introduction: Age estimation has an important role in the process of human identification and needs to be performed in an efficient, fast, and accurate ways. Age estimation based on the pulp cavity volume of canines was referring to the theory stated that the apposition of secondary dentine during human life could be determined as an age indicator. This study was aimed to estimate the human age based on the pulp volume of canines for chronological estimation. Methods: Forty-one canine teeth from Cone Beam Computed Tomography (CBCT) 3D image sample from Dental Hospital of the Faculty of Dentistry Universitas Padjadjaran segmented into dental pulp and enamel were prepared for measurement of pulp volume using the ITK SNAP volumetric software. Descriptive analysis of pulp volume and regression equations were generated from regression analysis of pulp volume and tested for age estimation. Result: Dentine thickness was found to be increased from adolescence age range until adulthood age range. Logarithmic regression between pulp volume was statistically significant (p < 0,001). The coefficient of determination (R2) was 0.753 with a standard deviation of 8.421. Mathematics model for estimating age was: Age = 79.523 – (18.194 x ln(Pulp Volume)). The pulp cavity and dentine thickness were decreasing along the age. The same equation was also used in the same sample. There were several differences between chronological age and estimated age approximately 8.4 years following the regression result of R2 (75.3%). The rest of the sample (24.7%) have a big difference, and this could be affected by modifying variables such as occlusal force and human error measurement procedures. Conclusion: The human age can be estimated by the pulp volume of canines.Keywords: Age estimation, pulp volume, canine, ITK-SNAP, CBCT.
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Davidovic, Lado, Mirjana Cuk, Marija Zivkovic-Sandic, Djurica Grga, and Slavoljub Zivkovic. "The influence of liners on the pulp inflammation." Srpski arhiv za celokupno lekarstvo 143, no. 5-6 (2015): 261–66. http://dx.doi.org/10.2298/sarh1506261d.

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Introduction. The study included application of liners and dental composites in to cavities of six experimental animals - rabbits (Oryctolagus cuniculus). Objective. The aim of the study was to investigate rabbit dental pulp response to different liners. Methods. Cavity preparation for class V were made on the maxillary central incisors and one lower incisor, while the second lower incisor served as a control tooth. These teeth were restored with the use of one of the following liners - Calcimol LC, ANA Liner and Fuji II LC Improved, and Ceram-X mono dental composite. After an observation period of five days animals were sacrificed and prepared for histological analysis. The existence and degree of the pulp inflammation was determined by using a light microscope. Results. Results showed that the used liners do not cause distortion of the structure and continuity of the odontoblastic layer. Inflammation was not registered in the control group, while in each group of tested materials one tooth with mild signs of hyperemia was registered. Results showed that all three tested liners demonstrated favorable effects on the pulp of the tooth and did not lead to inflammatory reactions. Conclusion. Histological analysis of the dental pulp of experimental animals suggests that the liners used in this study do not compromise the integrity of the odontoblastic layer, if it is applied over a thin layer of dentin. In each group of tested materials one tooth with mild signs of hyperemia and vasodilation was registered.
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Antonova, I. N., L. Yu Orekhova, T. B. Tkatchenko, S. B. Ulitovskiy, G. A. Khatskevich, and A. I. Yaremenko. "The scientific justification of the preparation's algorithm of the patients for oral prosthetics." Scientific Notes of the I. P. Pavlov St. Petersburg State Medical University 22, no. 2 (2015): 68–70. http://dx.doi.org/10.24884/1607-4181-2015-22-2-68-70.

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The therapeutic and diagnostic activity is a collective work that is carried out jointly by specialists in different directions of dentistry and support units. The main goal of this type of activity is to improve the dental health by preventing dental diseases. It includes the treatment and rehabilitation using various ways of replacement of the lost integrity of the dentition and restoration of the teeth using a prosthetic. In order to do this, it is necessary to prepare the teeth and the pulp of the oral cavity for prosthetics. The sequence of preparatory activities includes a series of steps carried out in sequence: preventive, hygienic, therapeutic (including periodontal), surgical and orthopedic (including orthodontics). Each of these stages includes a series of manipulations, the sequence and number of which is determined by the individual dental status of each patient. The inspection and preparation of a comprehensive plan for the treatment involves a sequence of all these actions carried out by a team of experts in agreement with the patient, evidenced by the «informed consent» subscription. After the preparatory activities, it is possible to go to the final stage, that is to make a prosthetic appliance to the patient. The total duration of all phases of preparatory activities preceding the prosthetics depends on the complexity of the steps themselves, and is determined by the severity of the related dental diseases. The treatment of these diseases is the main part of the preparatory activities.
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Nascimento, Bianca Mara Zakalusne do, Cristiano Zortéa, Monique Marchiori, et al. "Análise radiográfica das aberturas endodônticas de dentes de pacientes referenciados a um Centro de Especialidades Odontológicas Radiographic analysis of endodontic access of patients referenced to a Center of Dental Specialities." RSBO 16, no. 1 (2019): 22. http://dx.doi.org/10.21726/rsbo.v16i1.781.

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Introduction: Coronary access is the first stage of endodontic treatment and aims at direct access to root canals. Objective: To evaluate, by radiographic analysis, endodontic access cavities through radiographic analysis of the teeth of patients coming from primary care referred to the Center of Dental Specialties (CEO) Positivo, Curitiba, PR, Brazil. Material and methods: A total of 226 periapical radiographs taken previous to attending the CDS were analyzed, from August 2015 to August 2017. Three trained and calibrated examiners verified the following criteria: oral cavity region, presence of remaining caries,removal of roof of the pulp chamber, compensatory mesiodistal wear, iatrogenic (suggestive) and temporary restoration. Data were tabulated and analyzed for distribution, followed by bivariate analysis (Fisher’s Exact Test) with significance of 5%. Results: The posterior region prevailed in 70.3% of the sample. There remaining caries were found in 14.2%, insufficient roof removal in 25.2%, iatrogenic in 35.4%, and inadequate height of the temporary restoration in 11.5% of the cases. In the posterior teeth, insufficient mesiodistal compensatory wear was identified in 35.4 of the exams. The bivariate analysis revealed no significant difference when the variable region of the oral cavity was analyzed in relation to the presence of caries (p = 0.393), remaining roof (p = 0.056) and presence of iatrogenic (p = 0.304). Conclusion: It is concluded that there are relevant failures in the endodontic access received in the CDS, which indicates the need for professional training in primary care.
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Jain, Ashish R., R. Pradeep, Sashi Deepth Reddy Janapala, and P. Sesha Reddy. "The Effect of Three Dentinal Sealers on Retention of Crowns cemented with Resin-modified Glass Ionomer Cement: An in vitro Study." World Journal of Dentistry 6, no. 1 (2015): 10–15. http://dx.doi.org/10.5005/jp-journals-10015-1305.

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ABSTRACT Background Dentinal sealers (desensitizing agents) are used to protect the pulp from possible injurious effects after tooth preparation and also prevent the penetration of dentinal tubules by bacteria and their products which are currently thought to cause most of the pulpal inflammation under the crowns. Aim The purpose of this study is to determine the efficacy of effect of three different desensitizing agents on retention of crowns cemented with resin-modified glass ionomer cement. Materials and methods Forty freshly extracted maxillary first premolars were notched for retention and the teeth specimens were mounted in autopolymerising resin. The axial height of all the specimens was 4 mm with a 20° angle of convergence. Impressions of the prepared teeth were made, later waxed invested and casted. Thirty teeth were coated with three different desensitizing agents were used in this study: Cavity varnish (Namuvar, Deepti Dental Products), Glutaraldehyde (Gluma- Heraeus Kulzer), Resin (AdheSE, Ivoclar Vivadent). Ten teeth were not coated with desensitizing agents to act as control group. Independent T test used to compare the mean values between groups. Results Tensile strength for average surface area of the groups I, II, III and IV are 0.3759, 0.2375, 0.2411, 0.2348 respectively. The t-test shows ‘p’-value is statistically not significant (p < 0.05) for groups II and III, where as in group IV ‘p’-value is statistically significant (p < 0.01). Conclusion The use of AdheSE/resin-based dentinal sealer showed increase in bond strength of the crowns luted with resin-modified glass ionomer cement when compared with control group. This study advocates the use of resin-based sealer or a glutaraldehyde-based sealer before cementation of the crowns. The cavity varnish, however, reduces the bond strength and is not acceptable. How to cite this article Janapala SDR, Reddy PS, Jain AR, Pradeep R. The Effect of Three Dentinal Sealers on Retention of Crowns cemented with Resin-modified Glass Ionomer Cement: An in vitro Study. World J Dent 2015;6(1):10-15.
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Nurhapsari, Arlina. "PERBANDINGAN KEBOCORAN TEPI ANTARA RESTORASI RESIN KOMPOSIT TIPE BULK-FILL DAN TIPE PACKABLE DENGAN PENGGUNAAN SISTEM ADHESIF TOTAL ETCH DAN SELF ETCH." ODONTO : Dental Journal 3, no. 1 (2016): 8. http://dx.doi.org/10.30659/odj.3.1.8-13.

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Background: Composite resin has been widely used because of it's great aesthetic and physico-chemical properties. Type of posterior composite resins mostly used nowadays are packable and bulk-fill composite resins. Shrinkage in composite resin resulting microleakage that could lead to pulp sensitivity. Dentin bonding adhesive system can help to minimalize shrinkage. There are two type of adhesive system, the total etch and self etch group. The purpose of this study was to compare the microleakage between bulk-fill and packable composit resin with two different types of dentin bonding adhesive system. Method: twenty premolar teeth used as sample. The teeth were divided into four groups of 5 teeth each and Class I cavity preparations were prepared. The groups are : G1 (PC + TE), G2 (PC + SE), G3 (BC + TE), G4 (BC + SE). The specimens were thermocycled, stained with methylene blue dye, and sectioned to evaluate the dye penetration.Result: Kruskal Wallis test showed no difference among 4 groups. Meanwhile, Mann Whitney test showed significance difference between G2 - G3, and G3 - G4.Conclus/on: The study demonstrated that total etch has better sealing ability than self etch and there was no significant microleakage difference between bulkfill composite and packable composite.
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Manmontri, Chanika, Phattaranant May Mahasantipiya, and Papimon Chompu-inwai. "Preeruptive Intracoronal Radiolucencies: Detection and Nine Years Monitoring with a Series of Dental Radiographs." Case Reports in Dentistry 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/6261407.

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Preeruptive intracoronal radiolucencies (PEIRs) are mostly incidentally found by routine radiographic examination of unerupted teeth. PEIRs are classified into two types according to the nature of the lesion: progressive and nonprogressive. A case report of a 17-year-old boy with a nonprogressive PEIR on the permanent mandibular left second molar is presented. The lesion was initially detected on an unerupted tooth at age eight years, eight months. It was clinically and radiographically assessed yearly. Cone beam computed tomography (CBCT) was used to evaluate the lesion’s size and location when the patient was 11 and 14 years old. The assessments confirmed that the lesion was nonprogressive and had no connection to the pulp or oral cavity. Due to the static nature of the detected PEIR during the nine-year follow-up period, the patient’s low caries-risk status, and high patient and parental cooperation in periodic dental care, it was decided to place resin sealant on the affected tooth and monitor the lesion without any operative treatment.
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Vignesh S, Iffat Nasim, and Arvina Rajasekar. "A Retrospective Analysis of The Correlation Between the Type of Pulp Exposure and The Choice of Pulp Capping Agents." International Journal of Research in Pharmaceutical Sciences 11, SPL3 (2020): 901–6. http://dx.doi.org/10.26452/ijrps.v11ispl3.3046.

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The aim was mainly to determine the correlation between the type of pulp capping method and the material of choice in this study. Pulp capping in the carious teeth has been considered as unpredictable and they mainly have a lesser success rate. The pulp capping can be mainly done in two methods; they are direct and indirect pulp capping which can be mainly done with the help of different materials of choice like calcium hydroxide,MTA etc. The study was conducted in Saveetha Dental College . The data collection has been done from the department of Conservative dentistry and Endodontics for patients undergoing pulp capping treatment. A total sample data of 140 patients were obtained for a period of nine months ( June 2019 – April 2020.). Statistical analysis was done by using chi-square test with SPSS software version 23. The results were obtained and tabulated, the type of pulp capping method were 67% of indirect pulp capping and 33% of direct pulp capping method in which 81% of calcium hydroxide, 8% of MTA and 11% of RMGIC was used as the materials of choice. Among which indirect pulp capping method was most commonly used than the direct pulp capping method with calcium hydroxide as the material of choice for the treatment (p < 0.05 which is statistically significant). The pulpal exposure was mainly due to the dental caries and mechanical preparation of the tooth. Within the limits of the present study, the most common type of the pulp exposure was due to the dental caries and mechanical exposure during the cavity preparation of the tooth and the most common method of pulp capping was the indirect method with calcium hydroxide (CaOH) as the most common material of choice.
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Miranda Filho, Aluísio Eustáquio de Freitas, Diego Henrique da Silva Soares, Petrus Leonardo Alves dos Santos, et al. "Dental treatment in child with congenital heart disease under general anesthesia: Clinical case report." Research, Society and Development 10, no. 6 (2021): e56510615907. http://dx.doi.org/10.33448/rsd-v10i6.15907.

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Congenital heart diseases consist of structural defects of the heart and represent serious malformations, which manifest in newborns, contributing significantly to perinatal mortality. The dental management of patients with this condition may require caution by professionals, owing to the possibility of bleeding and the risk of causing an infectious endocarditis due to the variety of microorganisms residing in the oral cavity. The present case report aimed to detail dental care under general anesthesia in a hospital for a child with congenital heart disease. Female patient, 4 years old, was diagnosed with atrial and interventricular communication and hemodynamic repercussion 21 days after birth. The patient received dental treatment according to the proposed plan, which included dental restorations, conservative pulp therapy and extraction of lower anterior teeth. Posteriorly, a removable partial prosthesis was made for the patient for the purpose of functional aesthetic rehabilitation. The use of general anesthesia may reduce the risk of infective endocarditis and allows the performance of several dental procedures with a single antibiotic prophylaxis, since the cardiac pediatric patient needs a correct planning of the procedures to be performed.
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Amlani, Harsh. "Microleakage: Apical Seal vs Coronal Seal." World Journal of Dentistry 4, no. 2 (2013): 113–16. http://dx.doi.org/10.5005/jp-journals-10015-1215.

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ABSTRACT Microorganisms can reach the dental pulp through the open cavity, dentinal tubules, gingival sulcus, periodontal ligament through a broken occlusal seal or faulty restoration of tooth previously treated by endodontic therapy or extension of a periapical infection from adjacent infected teeth. During operative procedures, these possible paths must be considered to prevent ingress. While endodontic therapy is being performed, these paths must be blocked to avoid contamination during and after the treatment. Well treated tooth might also fail if microorganisms reach the periradicular tissues. After root canal obliteration, care must be taken to ensure maintenance of the coronal as well as apical seal from contaminants. How to cite this article Amlani H, Hegde V. Microleakage: Apical Seal vs Coronal Seal. World J Dent 2013;4(2):113-116.
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Anwarullah, Anupreeta, Ravi Kumar Konagala, Murali Krishna Raju, Amara Swapna Lingam, and Lakshman Varma U. "Endocrown - The Realm of Post-Free Endodontic Restorations." Journal of Evolution of Medical and Dental Sciences 10, no. 16 (2021): 1187–90. http://dx.doi.org/10.14260/jemds/2021/254.

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With increased dental awareness, there has been a growing trend in patients who are opting for teeth retention, so post-endodontic restorations play a significant role in dictating restoration longevity. However, till date restoration of root canal treated teeth is still controversial. Root canal treated teeth often suffer from extensive loss of tooth structure because of access cavity preparation, caries removal, replacement of restoration and trauma.1 Traditionally the restorative material of choice for an endodontically treated molar would be post and core followed by metal / ceramic crowns. However, studies have shown that these intra-canal posts may increase risk, of undermining the residual tooth structure, root perforation and catastrophic failures.2 With adhesive strategies, there has been a paradigm shift towards post-less restorations and utilisation of pulp chamber as an extension, thus integrating the crown and core as a single unit or monobloc. 2,3 This was the concept of the endocrown technique. 3 Bindle and Mormann first, delineated the use of porcelain adhesive endodontic crowns.4 Endocrowns are more practical, conservative, aesthetic, simple to fabricate, economical, less time consuming and allows favourable distribution of masticatory stresses. Most studies on endocrowns showed comparable or somewhat superior results to other conventional treatments for endodontically treated teeth (ETT). However, the available literature is based largely on the use of ceramic endocrowns. 2-4 It was observed that data pertaining to endocrown fabrication with other indirect restorative materials is still limited, and the choice is still unclear.Indirect composites show enhanced mechanical properties, creation of ideal contacts and contours, favourable wear, aesthetics and can serve as an economical alternative to ceramics.5 Hence, this paper attempts to present a case report of functional and aesthetic rehabilitation of an endodontically treated molar with an endocrown restoration utilising indirect composite (SR Adoro System).
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Souza-Rodrigues, Renata Duarte de, Bruna Puty, Laís Bonfim, et al. "Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings." PeerJ 9 (June 10, 2021): e11114. http://dx.doi.org/10.7717/peerj.11114.

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Background Methylmercury (MeHg) is a potent toxicant able to harm human health, and its main route of contamination is associated with the consumption of contaminated fish and other seafood. Moreover, dental amalgams are also associated with mercury release on human saliva and may contribute to the accumulation of systemic mercury. In this way, the oral cavity seems to be the primary location of exposure during MeHg contaminated food ingestion and dental procedures but there is a lack of literature about its effects on dental tissues and the impact of this toxicity on human health. In this way, this study aimed to analyze the effects of different doses of MeHg on human dental pulp stem cells after short-term exposure. Methods Dental pulp stem cells from human exfoliated deciduous teeth (SHED) were treated with 0.1, 2.5 and 5 µM of MeHg during 24 h. The MeHg effects were assessed by evaluating cell viability with Trypan blue exclusion assay. The metabolic viability was indirectly assessed by MTT reduction assay. In order to evaluate an indicative of antioxidant defense impairment, cells exposed to 0.1 and 5 µM MeHg were tested by measuring glutathione (GSH) level. Results It was observed that cell viability decreased significantly after exposure to 2.5 and 5 µM of MeHg, but the metabolic viability only decreased significantly at 5 µM MeHg exposure, accompanied by a significant decrease in GSH levels. These results suggest that an acute exposure of MeHg in concentrations higher than 2.5 µM has cytotoxic effects and reduction of antioxidant capacity on dental pulp stem cells.
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Toubes, Kênia Soares de, Stephanie Quadros Tonelli, Caroline Felipe Magalhães Girelli, et al. "Bio-C Repair - A New Bioceramic Material for Root Perforation Management: Two Case Reports." Brazilian Dental Journal 32, no. 1 (2021): 104–10. http://dx.doi.org/10.1590/0103-6440202103568.

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Abstract These case reports aimed to describe the management of lateral perforation in the middle cervical third of the root in two maxillary incisors with pulp canal calcification using Bio-C Repair, with safe and viable clinical treatment strategies. Digital radiographic exams were obtained with different angles and analyzed using different filters. Cone-beam computed tomography (CBCT) images were requested to show the actual position of the canal, location of the perforation, and guide the strategic planning of the case. Subsequently, cavity access was prepared with the aid of dental operating microscopy. After perforation was identified, granulation tissue was removed and the original canal was identified and then dressed with calcium hydroxide. In the second visit, the perforation was filled with Bio-C Repair and the canal system filled with gutta-percha points and a root canal sealer (Bio-C Sealer). The teeth were restored with glass fiber post, 4 mm beyond the perforation level, and provisory crowns. Both teeth treated as described above were functional and asymptomatic with a 1-year clinical and radiographic assessment. The Bio-C Repair is suggested as a new cement option for the management of lateral canal perforations, with effective results as observed after a one-year follow-up.
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