Dissertations / Theses on the topic 'Tenotomia'
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Tavares, Marcelo Rodrigo. "Efeito do laser terapêutico na cicatrização tendinosa: estudo experimental em ratos." Universidade de São Paulo, 2002. http://www.teses.usp.br/teses/disponiveis/82/82131/tde-08012003-141011/.
Full textLow-potency laser therapy has been used as a means of minimizing the hazardous effects of inflammation and accelerating healing in damaged tissues. This paper aims at observeving the alterations caused by diode AsGa (904nm), laser in the calcaneal tendon of rats. Thirty 235g (average weight) Wistar rats were divided into 2 groups one with 20 and other with 10 animals, all of which underwent right calcaneal tendon tenotomy. One group started laser treatment at a dosege of 4 J/cm 2 24hours after surgery, the other being used as control. The punctual method was employed and the appliance pen kept in direct contact with animal's skin. Both groups were divided into subgroups, the animals of which were sacrificed in different times on the 8 th and 15 th postoperative day, respectively. The tendon was excised and submitted to histology analysis using light microscopy, which revealed the presence of inflammatory cells, fibroblasts, vascularization and collagen deposition. There was a statistically significant difference (p <0.05) between the control and the treatment as far as collagen deposition at the presence of inflammatory cells are concerned. This study suggests that laser therapy accelerates healing in calcanean tenotomy in rats by increase collagen deposition both in the early and late parts of the treatment.
Souza, Virgolino Nóbrega Brunno. "Tenotomía vs no tenotomía de la Porción Larga del Bíceps en la sutura del manguito de los rotadores. Análisis clínico–funcional y valoración ecográfica." Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/669459.
Full textIntroduction: The role of LHB in the glenohumeral joint generates controversies among researchers. And that these functions are increased when there are rotator cuff injuries, in which compensatory hypertrophy may do an instability of the LHB that can also cause pain. One of the imaging tests in the diagnosis of these lesions is ultrasound. Currently, many authors propose the tenotomy of the LHB as a gesture associated with the repair of the rotator cuff to avoid postoperative pain. However, there is no evidence that this gesture improves the clinical results of the isolated suture. Objectives: - To compare the clinical and imaging results of the arthroscopic sutures of the rotator cuff with and without the LHB tenotomy - To analyze the situation and cross-sectional area of the LHB in the bicipital groove by means of an ultrasound study in cases with and without tenotomy. Methods: This is a prospective randomized single blind comparative study, carried out with a final total cases of 54 patient have been diagnosed with rupture of the supraspinatus tendon and injury of LHB by clinical exploration, by imaging and arthroscopy. The patients were randomly divided into two groups, TENOTOMY (n = 25) and NO TENOTOMY (n = 29), according to a centralized program of random numbers. In all patients, preoperative and postoperative clinical-functional tests were performed at 3, 6 and 12 months after arthroscopic surgery of supraspinatus tendon suture with or without associated LHB tenotomy. An ultrasound study was also carried out at 3 and 12 months post-operative. The inclusion criteria were: Age between 40 to 65 years; partial supraspinatus tears (≥ 50% of thickness); total supraspinatus tears ≤ 3 cm in the coronal plane; muscle atrophy inferior to Thomazeau stage 2; presence of the LHB in the bicipital groove. Results: In the clinical-functional results, a significant improvement was observed between the Constant and the ASES Test score preoperatively and post-operative in both groups (No tenotomy p <0.001 and Tenotomy p <0.001). No significant differences were observed in the Clinical-Functional Tests between the 2 groups studied (Tenotomy and No Tenotomy) at 12 months postoperatively (Constant Test p = 0.883 and ASES Test p = 0.716). The integrity of the supraspinatus tendon suture at 12 months showed no significant difference between both groups 12 months post-operative. No Tenotomy: 93.1% integrity of supraspinatus and Tenotomy: 88% integrity (Fisher's exact test, p = .653). The analysis of the presence of LHB in GB shows that at 12 months there is a significant difference between both groups, being higher in patients of the NO TENOTOMY group: 86.2% vs. 56% (Fisher's exact test, p =. 017). The cross-sectional area of the LHB in GB presented a significant decrease at the proximal and distal level of GB between the measurements taken at 3 and 12 months in both groups (No Tenotomy: proximal p = 0.021 and distal p = 0.026; Tenotomy: proximal p = 0.005 and distal p = 0.004). Conclusions: In conclusion there are no significant differences between both groups in the score of the Test of Constant and the ASES, being similar. It is confirmed that sutures of the supraspinatus tendon remain integrated 12 months post-operative in a high percentage of patients in both groups. The LHB remains in the GB in 56% of the Tenotomy patients and in 86.2% of the Non Tenotomy patients. The area of LHB at 12 months post-operative shows a significant decrease compared to 3 months post-operative in both groups.
Alves, Paulo Henrique de Matos. "Caracterização histoquímica das fibras do músculo gastrocnêmio de ratos Wistar submetidos à tenotomia e tenorrafia." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-01022012-143814/.
Full textTendon injuries are one of the most commonly occurring in sports, its frequency is 10 to 55% of all injuries. The medial head of gastrocnemius muscle (GCm) consists of two clearly distinct regions: a deep region \"red\" and, a superficial region \"white\". Because of that characteristic distribution of fiber types, it becomes possible to determine if the same muscle tenotomy affects differently, depending on the predominant fiber type in each region. The aim of this study was to evaluate the effect of experimental tenotomy and tenorrhaphy on GCm. We used 38 male Wistar rats weighing approximately 300 g divided into three groups: control (C), tenotomized (T) and Tenorraphized (R). They were evaluated at 14 and 21 days after the surgery. Animals were kept under the same conditions of accommodation, feeding, temperature, humidity and light. In T group, calcaneal tendon of the left pelvic limb was dissected and sectioned in the middle third. In R group, the same tendon was immediately submmitted to the modified Kessler suture after tenotomy. Sections were performed in 10 µm thick in a cryostat and they were then stained according to hematoxylin and eosin, Picrosirius, NADH-tr methods and they were analyzed by transmission electron microscopy. The statistical significance of inter-group differences was determined by analysis of variance (ANOVA) and was accepted p<0.05. Significant differences were found between C, T and R groups. There was a wide variation in size and shape of muscle fibers and also a disorientation and degeneration of myofibrils and disorganization of sarcomeres in T group. Both T and R groups showed a decrease in cross-sectional area and length of the GCm.
MORAES, Suellen Alessandra Soares de. "Inibição nitrérgica local favorece o processo de regeneração do tendão de aquiles em ratos submetidos à tenotomia." Universidade Federal do Pará, 2011. http://repositorio.ufpa.br/jspui/handle/2011/9289.
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A prevalência de lesões tendíneas tem aumentando pela constante exigência de movimentos repetitivos em atividades ocupacionais e recreacionais da sociedade. As principais características dessa injúria envolvem disfunção cicatricial e perda de matriz extracelular (ME). Nesse tecido, a ME é constituída basicamente por colágeno tipo I, e a perda dessa proteína aliada a problemas na remodelagem é considerado um dos principais responsáveis pelo quadro sintomatológico e funcional da injúria. Somando-se a isso, a literatura reporta que a expressão das óxido nítrico sintase estão reguladas positivamente ao longo do processo de reparo. Nesse contexto, a proposta do trabalho é averiguar a influência do sistema nitrérgico local na recuperação histológica e funcional do tendão. Para isso, o trabalho conta com o modelo experimental de ruptura do tendão de Aquiles, possibilitando instaurar um quadro de tendinopatia crônica nos animais. O acompanhamento dos grupos controle, ruptura+veículo (Salina 0,9%), ruptura+L-nitro-arginina-metil-éster (LNAME, 5mM) e ruptura+nitroprussiato de sódio (SNP, 3mM) prosseguiram até o 21° dia, estipulado para a coleta de amostras. A fim de avaliar a produção de óxido nítrico, o método de dosagem de Griess foi usado. Os parâmetros funcionais foram caracterizados pelo índice funcional de Aquiles (IFA), obtidos em 0, 7, 14 e 21 dias. Amostras também foram reservadas para processamento histomorfológico e autofluorescência. O ganho de massa corporal também foi avaliado. Conseguimos caracterizar o efeito das drogas no tecido sem fortes influências sistêmicas pela manutenção do peso corporal e níveis de nitrito plasmático. O L-NAME gerou uma significativa redução na densidade celular, inibiu a formação de vasos entre a malha colágena, melhorou a organização tecidual e restabeleceu precocemente o padrão funcional em 21 dias após a injúria. Por outro lado, os tratamentos com SNP e veículo mantiveram a desorganização do tecido e desempenho funcional insatisfatório. Juntos, nossos resultados sugerem um efeito benéfico da inibição local da NOS após a ruptura para a remodelagem tecidual, contribuindo para a regeneração do tendão.
The tendon injuries prevalence has been increased usually by repetitive movements in the occupational and recreational activities of society. The main features this injury involve healing dysfunction and loss of extracellular matrix. At this tissue, the extracellular matrix is basically made of type I collagen and the loss of this protein associated with problems in the remodeling have been considered the major responsible for the symptomatic and functional effects of injury. Additionally the literature reports the nitric oxide synthase expression was up-regulated throughout the repair process. At this context the propose of this work is investigated the nitrergic local inhibition in tissue and functional recovery after tendon injury. We used a experimental model of Achilles tendon rupture for establish pathophysiological changes similar to chronic tendinopathy. The animals were divided into experimental groups control, injury+vehicle (saline, 0,9%), injury+L- Nω-nitro-L-arginine methyl ester (L-NAME, 5mM) and injury+sodium nitroprusside (SNP, 3mM), which were followed until the 21º day, when were killed to collect samples. In order to access nitric oxide production Griess nitrite assay was used. Functional recovery was calculated by Achilles Functional Index (AFI) in 0, 7, 14 and 21 days. Tendons were also processed for histomorphological and autofluorescence analysis. The body weight gain was also evaluated. We characterized the effect of drugs about tissue without stronger systemic influences by maintenance of body weight and plasmatic levels of nitrite. The L-NAME led to significant decrease in the cellular density, vessel formation between collagen network, accelerated the tissue organization and restored early the functional pattern at 21 days after injury. On the other hand, the treatment with SNP and vehicle remained the tissue disorganization and low functional performance. Taken together our results suggest a positive effect of local suppression of NOS after rupture for tissue remodeling, contributing to tendon regeneration.
SEABRA, Adriene Damasceno. "O bloqueio da atividade nitrérgica no tendão de ratos submetidos à tenotomia com sutura acelera a regeneração muscular." Universidade Federal do Pará, 2012. http://repositorio.ufpa.br/jspui/handle/2011/3004.
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A atrofia é uma resposta imediata do músculo em situações de tensão e carga reduzida e caracteriza-se por mudanças morfológicas, aumento da proteólise muscular, perda de massa e redução da área da fibra que estão implicadas em déficits funcionais, afetando assim a qualidade de vida dos indivíduos. Rupturas tendíneas ocasionam atrofia muscular devido à intrínseca relação funcional existente entre ambas as estruturas, músculos-tendões. Considerando a injúria tendínea, trabalho prévio do nosso grupo identificou que a inibição da síntese de óxido nítrico localmente a injúria acelerou a recuperação histológica no tendão e a melhora funcional em animais tenotomizados. Desta forma, a proposta do trabalho é avaliar os efeitos da inibição nitrérgica local no tendão em relação ao padrão de regeneração muscular. Portanto, para gerar atrofia muscular esquelética, o trabalho utiliza o modelo experimental de ruptura do tendão calcâneo com posterior sutura. Os grupos foram divididos em controle, ruptura, ruptura+veículo (Salina 0,9%) e ruptura+L-nitro-arginina-metil-éster (L-NAME, 5mM). As amostras foram coletadas 14 e 21 dias seguintes ao procedimento cirúrgico experimental. Objetivando avaliar a dosagem de proteínas, o método de Bradford foi utilizado. As amostras também foram reservadas para processamento histológico qualitativo e quantitativo da área da fibra muscular e presença de lesões de núcleo central. Assim como no trabalho prévio de nosso grupo, acreditamos que a ação da droga restringiu-se ao local, pois não ocasionou fortes influências em relação ao peso corporal dos animais, que foi medido nos dias 0, 7, 14 e 21. O grupo tratado com L-NAME apresentou diminuição significativa no número de lesões de núcleo central no 14º dia pós-operatório e aumento nos níveis de proteína e área da fibra no 21º dia. Em conjunto, nossos resultados sugerem que houve efeito benéfico da inibição local da NOS após a ruptura com relação à atenuação da atrofia, contribuindo para acelerar a regeneração muscular.
Muscle atrophy is an immediate response of muscle under both situations of reduced tension and unloading. It is characterized by morphological changes, increase in muscular proteolysis, loss of mass and decrease in fiber area which are implicated in functional deficits, affecting the life quality of individuals. Tendon ruptures induce muscle atrophy due to intrinsic functional connection between both structures. Our previous work has shown that local inhibition of nitric oxide synthesis accelerated histological recovery in tendon and functional improvement in tenotomized animals. The present study was undertaken in order to evaluate the effects of local nitrergic inhibition on pattern of skeletal muscle regeneration after tenotomy. Skeletal muscle atrophy was induced by experimental rupture of the calcaneal tendon followed by suture. Animals were divided in the following groups, control, rupture, rupture + vehicle (0.9 % Saline) and rupture + L-nitroarginine methyl ester (5 mM L-NAME). Muscle samples were obtained 14 and 21 days after experimental surgical procedure. Total protein was measured by Bradford method. Samples were also used to qualitative and quantitative histological processing to determination of muscle fiber area and central core lesion. Animals were weighed at 0, 7, 14 and 21 days after surgery. Animals showed no significant differences in body weights, suggesting that drug had a local action. Group treated with L-NAME showed significant decrease in number of central core lesions at 14th postoperative day and increase in protein levels and fiber area at 21th day. Taken together our results suggest that local NOS inhibition had a beneficial effect after tendon rupture with regard to attenuation of atrophy, contributing for acceleration of muscle regeneration.
Oliveira, Anderson Rodrigues de. "Efeitos da terapia laser de baixa intensidade associada ao exerc?cio aer?bio no reparo de tenotomia do tend?o de aquiles de ratos diab?ticos." Universidade Federal do Rio Grande do Norte, 2016. http://repositorio.ufrn.br/handle/123456789/21101.
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Diabetes Mellitus (DM) ? um grupo de doen?as caracterizada por anormalidades metab?licas e complica??es cr?nicas. A hiperglicemia caracter?stica dessa doen?a produz glicosila??o de prote?nas e consequente ac?mulo de produtos finais da glicosila??o em v?rios tecidos humanos, entre eles, o tend?o. O exerc?cio aer?bio (EA) e a terapia laser de baixa intensidade (TLBI) t?m sido utilizados no manejo de tendinopatias em indiv?duos com ou sem DM. Objetivo: O objetivo desse estudo ? observar o efeito da TLBI e o EA, associados, no processo de reparo tecidual do tend?o de Aquiles (TA) de ratos diab?ticos. M?todos: Foram utilizados 91 animais divididos nos seguintes grupos: grupo controle (GC), grupo controle lesionado (GCL), grupo diab?tico (GD), grupo diab?tido laser (GDL), grupo diab?tico treinado (GDT) e grupo diab?tico treinado laser (GDTL). De acordo com o grupo, os ratos foram submetidos interven??o do EA, TLBI ou associa??o dos dois recursos. Foram analisadas as caracter?sticas morfol?gicas, biomec?nicas e moleculares. Resultados: O peso final dos animais diab?ticos foi estatisticamente menor quando comparado com os animiais controles (p <0,01). A glicemia dos animais diab?ticos foi estatisticamente maior quando comparado aos grupos controles (p<0,01). Os testes biomec?nicos indicaram melhoras significativas nos animais dos grupos GC e GDTL quando comparados aos grupos diab?ticos nas vari?veis de carga m?xima, distens?o, energia absorvida, stress, ?rea de sec??o transversa, m?dulo el?stico e densidade de energia (p<0,05). A an?lise por meio de biologia molecular indicou que a associa??o do exerc?cio aer?bio e Laser promoveu aumento da express?o g?nica de col?geno I e modulou a express?o das MMP2 e MMP9. N?o foi observada melhora significativa nas vari?veis morfol?gicas estudadas. Conclus?o: a TLBI associada ao exerc?cio aer?bio promove melhora nas propriedades mec?nicas e no controle da express?o g?nica do col?geno I e da MMP2 e MMP9 de ratos diab?ticos. Palavras-chave Diabetes Mellitus. Fototerapia. Exerc?cio. Tend?o de Aquiles. Cicatriza??o
Diabetes Mellitus (DM ) is a complex disease that requires continuous medical care for the reduction of risk factors in addition to glycemic control. The typical hyperglycemia of this disease produces glycosylation of proteins and so the consequence is the accumulation of glycosylation final products in various human tissues, among them, the tendon. The aerobic exercise (AE) and the low level laser therapy (LLLT) have been used to treat tendinopathies in individuals with or without DM. Objective: The aim of this study was to watch the effect of the LLLT and the AE, in association, in partial tenotomy of the tissue repair of the Achilles tendon (AT) of diabetic rats. Methods: 91 animals were utilized and divided in to the following groups: control group (GC), injured control group (GCL), diabetic group (GD), diabetic group LLLT (GD ? TLBI), diabetic group trained (GD - EX) and diabetic group trained laser (GD-EX+TLBI). The animals were submitted to intervention with AE, using a protocol with a progressive increase of time (12 to 60 min) and speed of (4 to 9 m/min), and the LLLT (660 nm laser, 10mW, 4 J/cm?, single point for 16 seconds, three times for week). It was analyzed morphological, biomechanical and molecular characteristics. For data showing normal distribution was used one-way ANOVA test and post hoc Tukey and data without normal distribution was used Mann Whitney test and post hoc Dunn's. It was accepted p <0.05 for statistical significance Results: The biomechanical tests indicated major improvement in the GC and GD-EX+TLBI groups when compared with the diabetic groups in the following variables: maximum load, strain, absorbed energy, stress, cross section area, elastic modulus and energy density (p<0.05). The analysis through molecular biology indicated that the association of aerobic exercise and LLLT generated an increase of the collagen I gene expression and modulated the expression of the MMP2 and MMP9 (p<0.05). No observed any major improvement in the morphological variable studied. Conclusion: the LLLT associated with aerobic exercise promotes and increase of the mechanical properties, in the control of collagen I gene expression and of the MMP2 and MMP9 of the diabetic rats.
Almeida, Marcos dos Santos de 1980. "O efeito da eletroacupuntura nos pontos Zusanli (E-36) e Chengshan (B-57) sobre o processo de cicatrização do tendão calcâneo de ratos após tenotomia parcial = uma análise bioquímica e morfológica." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317411.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: A eletroacupuntura (EA) tem sido utilizada amplamente para o tratamento de uma variedade de doenças inflamatórias e para o alivio da dor. No entanto, não há relatos na literatura sobre o efeito da EA na composição e organização da matriz extracelular (MEC) de tendões em processo de cicatrização. Objetivo: Investigar através de análises bioquímicas e morfológicas, o efeito da EA durante o pico de síntese dos componentes da MEC, 15 dias após transecção parcial do tendão calcâneo de ratos. Materiais e Métodos: Ratos Wistar machos com 60 dias de idade foram divididos em 3 grupos: Não tenotomizados (G1), tenotomizados (G2) e tenotomizados e submetidos a EA (G3). Agulhas de acupuntura (AC) foram inseridas nos pontos E-36 e B-57 e uma corrente elétrica com onda farádica bipolar assimétrica na freqüência de 2 Hz e intensidade de 2-4 Volts foi aplicada nas agulhas por 20 minutos, 3 vezes por semana em dias alternados, em um total de 6 sessões. Análises bioquímicas foram feitas através da SDS-PAGE, dosagem de proteínas não colagênicas (PNCs), glicosaminoglicanos (GAGs) e hidroxiprolina. Análise da metaloproteinase-2 (MMP-2) foi realizada por zimografia. Para análise morfológica em microscopia de luz comum, os cortes foram corados com hematoxilina-eosina (HE) e azul de toluidina (AT). Para análise em microscopia de polarização os cortes foram corados com Ponceau SS e a birrefringência foi avaliada em termos de valor de média de cinza em pixels utilizando um analisador de imagem. Resultados: EA nos pontos utilizados não alterou a concentração de PNCs, GAGs ou a atividade enzimática da MMP-2 nos tendões transeccionados. Já a concentração de hidroxiprolina foi significativamente aumentada nestes tendões quando tratados com EA. Na análise morfológica nos cortes corados com HE e AT, aparentemente não houve alterações na composição nem na organização da MEC. No entanto, na análise da birrefringência houve uma maior reorganização das fibras de colágeno no grupo tratado com EA. Conclusão: Nossos resultados indicam que a EA pode oferecer benefícios terapêuticos para o tratamento de lesões tendíneas através do aumento do conteúdo do colágeno e da reorganização molecular de suas fibras
Abstract: Electroacupuncture (EA) has been used to treat a variety of inflammatory diseases and pain relief. Therefore, there are no reports in the literature about the effect of EA on the composition and organization of the extracellular matrix (ECM) of tendons in the healing process. Objective: To investigate by morphological and biochemical analysis, the effect of EA during the peak of synthesis of ECM components, 15 days after partial transection of the Achilles tendon of rats. Methods: Male Wistar rats with 60 days old were divided into three groups: no tenotomized (G1), tenotomized (G2) and tenotomized and submitted to EA (G3). Acupuncture (AC) needles were inserted in the E-36 and B-57 points and an electrical current with asymmetrical bipolar faradic wave at frequency of 2 Hz and intensity of 2-4 Volts was applied to the needles for 20 minutes, 3 times per week on alternate days, for a total of 6 sessions. Biochemical analyses were done by SDS-PAGE, dosage of non collagenous proteins (NCPs), glycosaminoglycans (GAGs) and hydroxyproline. Analysis of metalloproteinase-2 (MMP-2) was carried out by zymography. For morphological analyses, the sections were stained with hematoxilin-eosin (HE) and toluidine blue. For polarization microscopy analysis the sections were stained with Ponceau SS and birefringence was evaluated in terms of gray average values in pixels using an image analyzer. Results: EA at points used did not alter the concentration of NCPs, GAGs or the enzymatic activity of MMP-2 in transected tendons. However, the concentration of hydroxyproline was significantly increased when these tendons were treated with EA. In the morphological analysis, apparently there were no changes in the composition and organization of the ECM. However, analysis of the birefringence showed higher reorganization of collagen fibers in the group treated with EA. Conclusion: Our results indicate that EA may offer therapeutic benefits for treatment of tendon injuries by increasing the content of collagen and the molecular reorganization of its fibers
Mestrado
Anatomia
Mestre em Biologia Celular e Estrutural
MAILLOT, JACKY. "Contribution au traitement des epicondylalgies du joueur de tennis : indication de la tenotomie sous-cutanee dans les epicondylites rebelles selon une technique modifiee." Besançon, 1990. http://www.theses.fr/1990BESA3100.
Full textMORAES, Suellen Alessandra Soares de. "Regeneração tendínea em modelo murino: estudo da plasticidade central e investigação do efeito da modulação nitrérgica na plasticidade periférica." Universidade Federal do Pará, 2015. http://repositorio.ufpa.br/jspui/handle/2011/7998.
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CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
FAPESPA - Fundação Amazônia de Amparo a Estudos e Pesquisas
As lesões tendíneas causam forte impacto sobre as pessoas em decorrência da dor e limitação funcional dela resultante. Após a lesão, o tecido passa a apresentar uma rede de nervos. Adicionalmente, há indícios da ocorrência de plasticidade central na medula após a lesão. Dentre os fatores moleculares envolvidos no reparo da lesão, o óxido nítrico (NO) é implicado na remodelagem tecidual, contudo seus efeitos ainda não são bem compreendidos. A proposta deste estudo é averiguar a existência de plasticidade central e a influência do NO na plasticidade periférica, limitação funcional e regeneração tendínea em modelo murino. Para estudar os efeitos do NO na plasticidade periférica, utilizamos animais controle (CTRL, sem lesão) ou tratados com salina (SAL, NaCl 0,9%), L-nitro-arginina-metil-éster (L-NAME, inibidor da síntese de NO) ou nitroprussiato de sódio (SNP, doador de NO) em dias alternados até o 21º dia pós-lesão (DPL). Para avaliar a ocorrência de plasticidade central (segmento L5), apenas a lesão foi realizada e a medula coletada em 2 ou 21 DPL. Analisamos a integridade e a organização tecidual nas amostras de tendão por H&E, microscopia eletrônica de transmissão e imunofluorescência, que também foi usada para avaliar a plasticidade periférica. Para verificar a recuperação funcional do tendão, determinamos o índice funcional de Aquiles, o ângulo articular e o campo aberto. No estudo da medula espinhal, investigamos a reatividade glial e o envolvimento neuronal após a injúria através de colocalizações com o indicador de ativação celular c-Fos. Os achados desta pesquisa mostram que a inibição do NO promove a organização tecidual em associação ao aumento da síntese, secreção e deposição de colágeno. Além disso, a administração local de L-NAME parece favorecer a diferenciação celular para tipos morfológicos análogos a tenócitos e melhorar a organização de ramos nervosos por dentre a malha de colágeno em correlação com a recuperação funcional em 21 DPL. Por outro lado, o aumento nos níveis de NO através de SNP promoveu uma piora em quase todos os parâmetros analisados. Nossos dados mostram ainda que a injúria tendínea desencadeia um processo de plasticidade central com aumento da reatividade glial em 2 DPL e da ativação celular ipsilateral à lesão em 2 e 21 DPL. Em suma, nossos achados indicam a ocorrência de plasticidade central após a lesão tendínea e o favorecimento do reparo tecidual e da plasticidade periférica através do bloqueio nitrérgico, revelando aspectos fundamentais da recuperação tecidual que podem representar novos alvos para uma nova abordagem terapêutica em lesões tendíneas.
Tendon injuries cause strong impact on people due to pain and functional limitation resulting therefrom. After injury, the tissue starts to present a network of nerves. Furthermore, there is evidence for the occurrence of central plasticity after injury. Among the molecular factors involved in injury repair, nitric oxide (NO) is implicated in tissue remodeling, but its effects are not yet well understood. The purpose of this study is to ascertain the existence of central plasticity and the influence of NO in peripheral plasticity, functional limitation and tendon regeneration in murine model. To study the effects of NO in peripheral plasticity, we used control animals (CTRL, without injury) or animals treated with saline (SAL, 0.9% NaCl), L-nitro-arginine-methyl-ester (L-NAME, NO-synthesis inhibitor) and sodium nitroprusside (SNP, NO donor) every other day until the 21st day post injury (DPI). To evaluate central plasticity (L5 segment), an injury was performed alone and the spinal cord collected at 2 or 21 DPI. We analyzed the integrity and tissue organization in tendon samples by H&E, transmission electron microscopy and immunofluorescence, which was also used to evaluate peripheral plasticity. To assess tendon functional recovery, we determined the Achilles functional index, the joint angle and the open field. In spinal cord studies, we investigated glial reactivity and neuron involvement after injury by co-localizations with the cell activation indicator c-Fos. The findings of this research show that NO inhibition promotes tissue organization in association to an increase in collagen synthesis, secretion and deposition. Besides, L-NAME local administration seems to favor cell differentiation to tenocyte-like morphological types and improve the organization of nerve branches in between the collagen mesh in correlation with functional recovery at 21 DPI. On the other hand, increased levels of NO by SNP promoted worsening in almost all parameters analyzed. Our data also show tendon injury triggers a central plasticity process with an increase in glial reactivity at 2 DPI and ipsilateral cell activation at 2 and 21 DPI. Afterall, our findings point out occurrence of central plasticity after tendon injury and favoring of tissue repair and peripheral plasticity through nitrergic blockage, unraveling fundamental aspects of tissue recovery that may represent new targets for a therapeutical approach in tendon injuries.
Nelson, Josh. "Surgical repair of a biceps tendon rupture a systematic review /." Morgantown, W. Va. : [West Virginia University Libraries], 2010. http://hdl.handle.net/10450/10936.
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Full textSwarts, Elfriede. "The outcome of prenatal sonographic diagnosis of fetal talipes in the Cape Town Metro district." Master's thesis, University of Cape Town, 2017. http://hdl.handle.net/11427/27550.
Full textKlein, Annette [Verfasser]. "Die Tenotomie der Flexor-hallucis-longus-Sehne und Flexor-digitorum-longus-Sehne zur Behandlung von Zehenkuppenläsionen bei Patienten mit Diabetischem Fußsyndrom / Annette Klein." Köln : Deutsche Zentralbibliothek für Medizin, 2012. http://d-nb.info/1022520490/34.
Full textWöhrle, Katharina [Verfasser], and Stephan [Akademischer Betreuer] Grechenig. "Eine neue implantatfreie Technik zur Tenodese der langen Bizepssehne (Loop Tenodese) im Vergleich zur Tenotomie. Eine biomechanische Analyse / Katharina Wöhrle ; Betreuer: Stephan Grechenig." Regensburg : Universitätsbibliothek Regensburg, 2020. http://d-nb.info/122008039X/34.
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Full textVaranda, João Pedro Folhas. "Hérnia do Desportista, fisiopatologia e modalidades terapêuticas." Master's thesis, 2018. http://hdl.handle.net/10316/82649.
Full textResumoIntrodução A hérnia do desportista ou pubalgia do atleta tem recebido crescente atenção como uma fonte de invalidez e tempo despendido pelos atletas. É uma condição de dor crónica inguinal em atletas, com uma incidência de 0,5 a 6,2%. É estimado que 5 a 18% de todas as lesões desportivas estejam relacionadas com a região inguinal. É mais frequentemente encontrada em atletas que praticam desportos envolvendo movimentos pélvicos de torção enquanto correm a alta velocidade como futebol, hóquei no gelo ou rugby. Foi atribuída a uma panóplia de diagnósticos como hérnia, hérnia incipiente,ponta de hérnia ou pubalgia. Não há consenso quanto a esta condição, especialmente na sua nomenclatura, situação que se tem revelado um desafio para a realização de um diagnóstico preciso, e consequentemente, na instituição do tratamento adequado.Material e MétodosEfectuou-se pesquisa nas bases de dados electrónicas PUBMED e INDEX das revistas médicas portuguesas disponíveis em www.ncbi.nlm.nih.gov/pubmed/ ewww.indexonline.pt, com as palavras-chave: sportsman´s groin, athletic pubalgia,inguinal disruption e groin pain. Foram identificados cerca de 1400 artigos, e após filtragem para o tipo de artigo (excluindo editoriais, guidelines, meta-análises, artigos de jornal e case-reports), ano de publicação, idioma e acessibilidade, apenas 120 eram realmente relacionados com o tema em estudo. Após a sua leitura foram selecionados 52 artigos, em função da atualidade da publicação e da adequação potencial ao tema, que constam na bibliografia. DesenvolvimentoDisrupção Inguinal (ID) pode ser definida como dor, de carácter agudo ou crónico, que ocorre predominantemente na região inguinal, perto do tubérculo púbico,onde não existe nenhuma outra patologia evidente, como uma hérnia inguinal verdadeira, que explique a sintomatologia. O diagnóstico clínico pode ser feito com pelo menos três dos cinco sinais abaixo descritos:1. Sensibilidade localizada (Pinpoint tenderness) sobre o tubérculo púbico no ponto de inserção do tendão conjunto;2. Sensibilidade (tenderness) palpável sobre o anel inguinal profundo;3. Dor ou dilatação do anel inguinal profundo, sem hérnia obviamente evidente;4. Dor na origem do tendão do longo adutor;5. Dor ou desconforto difuso na virilha, geralmente irradiando para o períneo, face interna da coxa, ou através da linha média.Na ID, a parede posterior do canal inguinal, composta exclusivamente pela fascia transversalis, está enfraquecida. Alguns atletas poderão apresentar também disrupção da aponevrose do músculo oblíquo externo. O achado mais frequente, em 85% dos atletas é uma parede posterior do canal inguinal enfraquecida. A RM é o exame de eleição para o diagnóstico de ID. A sua utilidade reside no facto de permitir a exclusão de outras patologias como dano acetabular, patologia da anca, ruturas musculares e lesões no osso púbico. A correcção cirúrgica nem sempre está indicada, e a fisioterapia e reabilitação são fortemente recomendadas para todos os atletas em primeira instância.Os objectivos-chave são: libertar a tensão anormal presente no canal inguinal e reconstruir a sua parede posterior com auxílio de uma prótese ou sutura simples, para suplantar a fragilidade. Nenhum procedimento cirúrgico demonstrou eficácia superior, com a abordagem aberta e laparoscópica a obterem excelentes resultados.Recomenda-se uma tenotomia do adutor combinado com reparação herniária,sempre que a sintomatologia das anormalidades do adutor não possam ser corrigidaspreoperatoriamente.ConclusãoA avaliação do atleta requer uma abordagem multidisciplinar, desde o treinador,preparador físico, fisioterapeuta, fisiatra, imagiologista, cirurgião geral e eventualmente um ortopedista. Para atletas com um diagnóstico de hérnia do desportista, que reportem insucesso após instituição de medidas conservadoras, devem ser consideradas várias opções cirúrgicas. Nos atletas sintomáticos, sem achados relevantes ao exame físico ou nos exames complementares de diagnóstico, será lícito realizar exploração cirúrgica e consequentemente reparação da parede abdominal e/ou tenotomia dos adutores, com neurotomia do nervo ilioinguinal e ramo genital do nervo genitofemoral, como solução para a dor. Estudos que comparam a eficácia da abordagem aberta e laparoscópica, são escassos ou inexistentes no entanto destaca-se a vantagem clara das técnicas cirúrgicas,nomeadamente laparoscópicas, quanto ao tempo diminuto de retorno às actividades desportivas. A tenotomia dos adutores tem ganho destaque pela sua capacidade de resolução do quadro álgico, quando associada ao reforço da parede posterior do canal inguinal com a utilização de uma prótese.
AbstractIntroductionThe athlete's athletic hernia or pubalgia has received increasing attention as a source of disability and time spent by athletes. It is a condition of chronic inguinal pain in athletes, with an incidence of 0.5 to 6.2%. It is estimated that 5 to 18% of all sports injuries are related to the inguinal region. It is most often found in athletes who engage in sports involving pelvic torsion movements while running at high speeds such as football, ice hockey or rugby. It is attributed to a panoply of diagnoses such as hernia,incipient hernia, hernia tip or pubalgia. There is no consensus on this condition,especially in its nomenclature, which has proved to be a challenge for a precise diagnosis and, consequently, for the institution of appropriate treatment.Materials and MethodsWe searched the electronic databases PUBMED and INDEX of Portuguese medical journals available at www.ncbi.nlm.nih.gov/pubmed/ and www.indexonline.pt, with the key words: sportsman's groin, athletic pubalgia , inguinal disruption and groin pain. About 1400 articles were identified, and after filtering for the type of article (excluding editorials, guidelines, meta-analyzes, journal articles and case reports), year of publication, language and accessibility, only 120 were actually related to the topic in study. After its reading, 52 articles were selected, depending on the current publication and the potential adequacy to the theme, which are included in the bibliography.DevelopmentID can be defined as acute or chronic pain, occurring predominantly in the inguinal region, near the pubic tubercle, where there is no other obvious pathology, such as a true inguinal hernia, which explains the symptomatology. Clinical diagnosis can be made with at least three of the five signs described below:1. Pinpoint tenderness on the pubic tubercle at the point of insertion of the tendon conjoint;2. Palpable tenderness on the deep inguinal ring;3. Pain or dilation of the deep inguinal ring, with no obvious hernia;4. Pain in the origin of the tendon of the long adductor;5. Diffuse pain or discomfort in the groin, usually radiating to the perineum, inner thigh,or through the midline.In ID, the posterior wall of the inguinal canal, composed exclusively of the transversalis fascia, is weakened. Some athletes may also present disruption of external oblique muscle aponeurosis. The most frequent finding in 85% of athletes is a weakened posterior wall of the inguinal canal. MRI is the exam of choice for the diagnosis of ID. Its usefulness is that it allows the exclusion of other pathologies such as acetabular damage, hip pathology, muscular ruptures and lesions in the pubic bone. Surgical correction is not always indicated, and physiotherapy and rehabilitation are strongly recommended for all athletes in the first instance. The key objectives are: to release the abnormal tension present in the inguinal canal and reconstruct its posterior wall with the aid of a simple prosthesis or suture, to overcome the fragility. No surgical procedure demonstrated superior efficacy, with the open and laparoscopic approach achieving excellent results. Adductor tenotomy combined with hernia repair is recommended,provided that the symptomatology of adductor abnormalities cannot be corrected preoperatively.ConclusionThe evaluation of the athlete requires a multidisciplinary approach, from the coach, physical trainer, physiotherapist, physiatrist, imaging, general surgeon and eventually an orthopedist. For athletes with a hernia diagnosis who report failure after institution of conservative measures, several surgical options should be considered. In symptomatic athletes, with no findings relevant to the physical examination or complementary diagnostic exams, surgical exploration and consequent repair of the abdominal wall and / or tenotomy of the adductors, with ilioinguinal nerve neurotomyand genitofemoral genital branch, may be performed as a solution for the pain. Studies comparing the efficacy of the open and laparoscopic approach are scarce or nonexistent. However, the clear advantage of surgical techniques, particularly laparoscopic ones, is less time to return to sports activities. The tenotomy of the adductors has gained prominence due to its ability to resolve the pain when associated with the reinforcement of the posterior wall of the inguinal canal with the use of prosthesis.