Relevant bibliographies by topics / Tet-On 3G

Contents

  1. Journal articles

Academic literature on the topic 'Tet-On 3G'

Author: Grafiati
Published: 7 June 2025
Last updated: 16 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Tet-On 3G.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Tet-On 3G"

1

Zhou, Yicheng, Chaoliang Lei, and Zhihui Zhu. "A low-background Tet-On system based on post-transcriptional regulation using Csy4." PLOS ONE 15, no. 12 (2020): e0244732. http://dx.doi.org/10.1371/journal.pone.0244732.

Full text
Abstract:
On account of its stringent regulation and high rate of induction, the tetracycline regulatory system is used extensively for inducing target gene expression in eukaryotes. However, under certain circumstances, its associated background expression can be problematic, as in the expression of highly toxic proteins. We found that when using the Tet-On 3G system to drive expression of the kid toxin gene in sf9 insect cells, a higher percentage of cells were killed than when using an empty vector in the absence of the induction agent doxycycline, thereby indicating the leaky expression of this indu
APA, Harvard, Vancouver, ISO, and other styles
2

Fan, Xiujun, Matthew Petitt, Matthew Gamboa, et al. "Transient, Inducible, Placenta-Specific Gene Expression in Mice." Endocrinology 153, no. 11 (2012): 5637–44. http://dx.doi.org/10.1210/en.2012-1556.

Full text
Abstract:
Abstract Molecular understanding of placental functions and pregnancy disorders is limited by the absence of methods for placenta-specific gene manipulation. Although persistent placenta-specific gene expression has been achieved by lentivirus-based gene delivery methods, developmentally and physiologically important placental genes have highly stage-specific functions, requiring controllable, transient expression systems for functional analysis. Here, we describe an inducible, placenta-specific gene expression system that enables high-level, transient transgene expression and monitoring of ge
APA, Harvard, Vancouver, ISO, and other styles
3

Ходарович, Ю. М., Д. Д. Рахманинова, А. М. Барышникова та С. М. Деев. "Регулируемый доксициклином двухпропромоторный интегратор на основе трансактиватора системы TET-ON 3G". Молекулярная биология 54, № 2 (2020): 308–12. http://dx.doi.org/10.31857/s0026898420020056.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Khodarovich, Yu M., D. D. Rakhmaninova, A. M. Barishnikova, and S. M. Deyev. "Doxycycline Sensitive Two-Promoter Integrator Based on the TET-ON 3G Transactivator." Molecular Biology 54, no. 2 (2020): 269–73. http://dx.doi.org/10.1134/s0026893320020053.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Wu, Lipeng, Hua Su, Justin Fellows, et al. "Abstract 3727: Development of a new All-In-One inducible lentiviral shRNA/gRNA Vector." Cancer Research 83, no. 7_Supplement (2023): 3727. http://dx.doi.org/10.1158/1538-7445.am2023-3727.

Full text
Abstract:
Abstract Tet-On is a powerful inducible system and a classical tool to regulate gene expression in mammalian cells. It has also been applied to regulate Pol III-driven transcription, such as shRNA or gRNA driven by a U6 or H1 promoter. However, all of the current versions of Tet-On shRNA vectors are based on H1-2O2 or U6-2O2 promoters, which are only compatible with first-generation tetracycline repressor TetR. In addition, these promoters have the problem of driving downstream transcription without the binding of the Tet regulatory protein. Here, we developed a new system that is built upon t
APA, Harvard, Vancouver, ISO, and other styles
6

Guichardaz, Michelle, Sveva Bottini, Elisa Balmas, and Alessandro Bertero. "Overcoming the Silencing of Doxycycline-Inducible Promoters in hiPSC-derived Cardiomyocytes." Open Research Europe 4 (December 18, 2024): 266. https://doi.org/10.12688/openreseurope.19024.1.

Full text
Abstract:
Background Human induced pluripotent stem cells (hiPSCs) are pivotal for studying human development, modeling diseases, and advancing regenerative medicine. Effective control of transgene expression is crucial to achieve temporal and quantitative precision in all of these contexts. The doxycycline (dox)-inducible OPTi-OX system, which integrates the Tet-On 3G transactivator and dox-responsive transgene at the hROSA26 and AAVS1 genomic safe harbors (GSHs), respectively, offers a promising solution. Yet, transgene silencing, particularly in hiPSC-derived cardiomyocytes (hiPSC-CMs), limits its ut
APA, Harvard, Vancouver, ISO, and other styles
7

Negron, Ariel L., Jon E. Levine, and Sally Radovick. "Development of a Novel Tetracycline-Inducible Kiss1-Cre Mouse Line for Temporally Controlled Gene Deletion in Kisspeptin Neurons." Journal of the Endocrine Society 5, Supplement_1 (2021): A535. http://dx.doi.org/10.1210/jendso/bvab048.1090.

Full text
Abstract:
Abstract The tetracycline (Tet)-controlled inducible system is the most widely used reversible system for transgenic expression in mice. Previously, we generated a GnRH-CreTeR mouse model, using a first-generation Tet-inducible system to temporally induce expression of Cre recombinase in GnRH neurons. Recently, the Tet-inducible system has undergone several modifications to significantly reduce previous limitations that include leaky background expression and lower sensitivity to tetracycline induction. Therefore, we have developed a novel mouse model bearing a Tet-inducible kisspeptin-Cre all
APA, Harvard, Vancouver, ISO, and other styles
8

Abe, Takuya, and Dana Branzei. "High levels of BRC4 induced by a Tet-On 3G system suppress DNA repair and impair cell proliferation in vertebrate cells." DNA Repair 22 (October 2014): 153–64. http://dx.doi.org/10.1016/j.dnarep.2014.08.003.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Alanazi, Samar M., Rosalin Mishra, Hima Patel, Long Yuan, Mary Kate Kilroy, and Joan T. Garrett. "Abstract P5-10-05: HER2 inhibition increases non-muscle myosin IIa to promote tumorigenesis in HER2+ breast cancers." Cancer Research 82, no. 4_Supplement (2022): P5–10–05—P5–10–05. http://dx.doi.org/10.1158/1538-7445.sabcs21-p5-10-05.

Full text
Abstract:
Abstract HER2 is amplified in about 20% of breast cancers. HER3 is as essential as HER2 for maintaining cell viability in HER2+ breast cancer cells. Inhibition of HER2 tyrosine kinase activity results in upregulation of HER3 transcription and phosphorylation. We sought to identify HER3 binding partners upon pharmacological inhibition of HER2 using the irreversible pan HER inhibitor neratinib. We immunoaffinity-purified HER3 from HER2+ BT474 cells treated ± neratinib. Following immunoprecipitation using a HER3 antibody, binding partners were released under reducing conditions. Mass spectrometry
APA, Harvard, Vancouver, ISO, and other styles
10

Bai, Yinshan, Cui Zhu, Meiying Feng, et al. "Establishment of A Reversibly Inducible Porcine Granulosa Cell Line." Cells 9, no. 1 (2020): 156. http://dx.doi.org/10.3390/cells9010156.

Full text
Abstract:
Granulosa cells (GCs) are the key components of ovarian follicles for regulating oocyte maturation. Previous established GC lines have allowed prolonged proliferation, but lost some physiological features owing to long-term immortalization. This study was to establish an induced immortal porcine GC line with reversible proliferation status by the tetracycline inducible (Tet-on) 3G system. Our conditional immortal porcine GCs (CIPGCs) line steadily propagated for at least six months and displayed primary GC morphology when cultured in the presence of 50 ng/mL doxycycline [Dox (+)]. Upon Dox wit
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography