Academic literature on the topic 'Tetracycline Repressor'

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Journal articles on the topic "Tetracycline Repressor"

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Kaszycki, Paweł, Andrzej Guz, Monika Drwięga, and Zygmunt Wasylewski. "Tet repressor-tetracycline interaction." Journal of Protein Chemistry 15, no. 7 (1996): 607–19. http://dx.doi.org/10.1007/bf01886743.

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Kim, H. J., C. Gatz, W. Hillen, and T. R. Jones. "Tetracycline repressor-regulated gene repression in recombinant human cytomegalovirus." Journal of virology 69, no. 4 (1995): 2565–73. http://dx.doi.org/10.1128/jvi.69.4.2565-2573.1995.

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Kamionka, Annette, Miriam Sehnal, Oliver Scholz, and Wolfgang Hillen. "Independent Regulation of Two Genes in Escherichia coli by Tetracyclines and Tet Repressor Variants." Journal of Bacteriology 186, no. 13 (2004): 4399–401. http://dx.doi.org/10.1128/jb.186.13.4399-4401.2004.

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ABSTRACT We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator (tetO) recognition and exclusive induction with the novel inducer 4-dedimethylamino-anhydrotetracycline. The other Tet repressor variant shows tetO-4C recognition and induction with tetracycline. We demonstrate that both variants are independently active in vivo and allow selective regulation of two genes in the same cell without any cross talk.
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Guo, Xinzheng V., Mercedes Monteleone, Marcus Klotzsche, et al. "Silencing Essential Protein Secretion in Mycobacterium smegmatis by Using Tetracycline Repressors." Journal of Bacteriology 189, no. 13 (2007): 4614–23. http://dx.doi.org/10.1128/jb.00216-07.

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ABSTRACT Many processes that are essential for mycobacterial growth are poorly understood. To facilitate genetic analyses of such processes in mycobacteria, we and others have developed regulated expression systems that are repressed by a tetracycline repressor (TetR) and induced with tetracyclines, permitting the construction of conditional mutants of essential genes. A disadvantage of these systems is that tetracyclines function as transcriptional inducers and have to be removed to initiate gene silencing. Recently, reverse TetR mutants were identified that require tetracyclines as corepress
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Palm, Gottfried Julius, Ina Buchholz, Sebastiaan Werten, et al. "Thermodynamics, cooperativity and stability of the tetracycline repressor (TetR) upon tetracycline binding." Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1868, no. 6 (2020): 140404. http://dx.doi.org/10.1016/j.bbapap.2020.140404.

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Dingermann, T., H. Werner, A. Schütz, et al. "Establishment of a system for conditional gene expression using an inducible tRNA suppressor gene." Molecular and Cellular Biology 12, no. 9 (1992): 4038–45. http://dx.doi.org/10.1128/mcb.12.9.4038.

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We investigated the use of the prokaryotic tetracycline operator-repressor system as a regulatory device to control the expression of Dictyostelium discoideum tRNA genes. The tetO1 operator fragment was inserted at three different positions in front of a tRNA(Glu) (Am) suppressor gene from D. discoideum, and the tetracycline repressor gene was expressed under the control of a constitutive actin 6 promoter. The effectiveness of this approach was determined by monitoring the expression of a beta-galactosidase gene engineered to contain a stop codon that could be suppressed by the tRNA. When thes
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Dingermann, T., H. Werner, A. Schütz, et al. "Establishment of a system for conditional gene expression using an inducible tRNA suppressor gene." Molecular and Cellular Biology 12, no. 9 (1992): 4038–45. http://dx.doi.org/10.1128/mcb.12.9.4038-4045.1992.

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We investigated the use of the prokaryotic tetracycline operator-repressor system as a regulatory device to control the expression of Dictyostelium discoideum tRNA genes. The tetO1 operator fragment was inserted at three different positions in front of a tRNA(Glu) (Am) suppressor gene from D. discoideum, and the tetracycline repressor gene was expressed under the control of a constitutive actin 6 promoter. The effectiveness of this approach was determined by monitoring the expression of a beta-galactosidase gene engineered to contain a stop codon that could be suppressed by the tRNA. When thes
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Degenkolb, J., M. Takahashi, G. A. Ellestad, and W. Hillen. "Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor." Antimicrobial Agents and Chemotherapy 35, no. 8 (1991): 1591–95. http://dx.doi.org/10.1128/aac.35.8.1591.

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Moon, Jayoung, Jongsik Gam, Seung-Goo Lee, Young-Ger Suh, and Jeeyeon Lee. "Light-Regulated Tetracycline Binding to the Tet Repressor." Chemistry - A European Journal 20, no. 9 (2014): 2508–14. http://dx.doi.org/10.1002/chem.201304027.

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Kedracka-Krok, Sylwia, and Zygmunt Wasylewski. "A differential scanning calorimetry study of tetracycline repressor." European Journal of Biochemistry 270, no. 22 (2003): 4564–73. http://dx.doi.org/10.1046/j.1432-1033.2003.03856.x.

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Dissertations / Theses on the topic "Tetracycline Repressor"

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Leng, Zhongtai. "Distribution and mobility of antibiotic resistant genes in oral/urogentital [sic] bacteria." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/9273.

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Harris, Andreas William Kisling. "The design of gene regulatory networks with feedback and small non-coding RNA." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:e3a323b1-9067-415d-8728-6c70c1b6cf23.

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The objective of the field of Synthetic Biology is to implement novel functionalities in a biological context or redesign existing biological systems. To achieve this, it employs tried and tested engineering principles, such as standardisation and the design-build-test cycle. A crucial part of this process is the convergence of modelling and experiment. The aim of this thesis is to improve the design principles employed by Synthetic Biology in the context of Gene Regulatory Networks (GRNs). Small Ribonucleic Acids (sRNAs), in particular, are focussed on as a mechanism for post-transcriptional
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Volkers, Gesa [Verfasser]. "Röntgenkristallographische Untersuchungen zum enzymatischen Abbau von Tetracyclinen durch die Monooxygenase TetX sowie zur Erkennung natürlicher Tetracyclin-Abbauprodukte durch den Tet-Repressor / Gesa Volkers." Greifswald : Universitätsbibliothek Greifswald, 2011. http://d-nb.info/1016938314/34.

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Reichheld, Sean. "The Role of Ligand Induced Stabilization in the Allosteric Mechanism of Tetracycline Repressor." Thesis, 2008. http://hdl.handle.net/1807/17242.

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Allosteric regulation of proteins by reversible ligand binding is essential for regulation of fundamental biological processes. The mechanism by which a binding event alters the function of a distant site in a protein is only poorly understood. In this thesis, I use the Tetracycline Repressor (TetR) as a model system to study ligand induced allostery. The transcription of genes encoding the resistance to the antibiotic, tetracycline (Tc), is repressed by TetR, which is a homodimeric alpha-helical protein possessing a small N-terminal DNA binding domain (DNB domain) and a larger C-terminal tetr
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Resch, Marcus [Verfasser]. "Strukturbiologie der Transkriptionsregulatoren YvoA und der reversen Tetracyclin-Repressor-Variante L17G = Structural biology of the transcription regulators YvoA and the reverse tetracycline repressor variant L17G / vorgelegt von Marcus Resch." 2009. http://d-nb.info/999033549/34.

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Othersen, Olaf G. [Verfasser]. "Inducers and induction of the tetracycline repressor protein : computational studies / vorgelegt von Olaf G. Othersen." 2007. http://d-nb.info/986220507/34.

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Seidel, Ute [Verfasser]. "Analysis of the mechanism of induction of selected tetracycline repressor variants using molecular dynamics simulations and protein-ligand docking / vorgelegt von Ute Seidel." 2008. http://d-nb.info/988606097/34.

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Books on the topic "Tetracycline Repressor"

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Litingtung, Ying. Differential regulation of the tetR repressor gene and the tetA tetracycline resistance gene in transposon Tn10 by the two tandem tet operators. 1990.

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Book chapters on the topic "Tetracycline Repressor"

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Hinrichs, W., P. Orth, C. Kisker, D. Schnappinger, W. Hillen, and W. Saenger. "Tetracycline Repressor Acts as a Molecular Switch Regulated by Tetracycline Binding." In Current Challenges on Large Supramolecular Assemblies. Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-5284-6_24.

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Moncivais, Kathryn, and Zhiwen Jonathan Zhang. "Tetracycline Repressor-Based Mammalian Two-Hybrid Systems." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-455-1_15.

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Hinrichs, Winfried, and Christiane Fenske. "Gene regulation by the tetracycline-inducible Tet repressor-operator system — molecular mechanisms at atomic resolution." In Tetracyclines in Biology, Chemistry and Medicine. Birkhäuser Basel, 2001. http://dx.doi.org/10.1007/978-3-0348-8306-1_3.

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Jacot, Damien, and Dominique Soldati-Favre. "CRISPR/Cas9-Mediated Generation of Tetracycline Repressor-Based Inducible Knockdown in Toxoplasma gondii." In Methods in Molecular Biology. Springer US, 2019. http://dx.doi.org/10.1007/978-1-4939-9857-9_7.

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Saenger, Wolfram, Dirk Kostrewa, Joachim Granzin, et al. "DNA Binding and Bending by two different Proteins: Factor for Inversion Stimulation (FIS) and Tetracycline Repressor (TetR)." In From Simplicity to Complexity in Chemistry — and Beyond. Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-49368-3_5.

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