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Journal articles on the topic 'Tetracycline Repressor'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

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1

Kaszycki, Paweł, Andrzej Guz, Monika Drwięga, and Zygmunt Wasylewski. "Tet repressor-tetracycline interaction." Journal of Protein Chemistry 15, no. 7 (1996): 607–19. http://dx.doi.org/10.1007/bf01886743.

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2

Kim, H. J., C. Gatz, W. Hillen, and T. R. Jones. "Tetracycline repressor-regulated gene repression in recombinant human cytomegalovirus." Journal of virology 69, no. 4 (1995): 2565–73. http://dx.doi.org/10.1128/jvi.69.4.2565-2573.1995.

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3

Kamionka, Annette, Miriam Sehnal, Oliver Scholz, and Wolfgang Hillen. "Independent Regulation of Two Genes in Escherichia coli by Tetracyclines and Tet Repressor Variants." Journal of Bacteriology 186, no. 13 (2004): 4399–401. http://dx.doi.org/10.1128/jb.186.13.4399-4401.2004.

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ABSTRACT We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator (tetO) recognition and exclusive induction with the novel inducer 4-dedimethylamino-anhydrotetracycline. The other Tet repressor variant shows tetO-4C recognition and induction with tetracycline. We demonstrate that both variants are independently active in vivo and allow selective regulation of two genes in the same cell without any cross talk.

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4

Guo, Xinzheng V., Mercedes Monteleone, Marcus Klotzsche, et al. "Silencing Essential Protein Secretion in Mycobacterium smegmatis by Using Tetracycline Repressors." Journal of Bacteriology 189, no. 13 (2007): 4614–23. http://dx.doi.org/10.1128/jb.00216-07.

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ABSTRACT Many processes that are essential for mycobacterial growth are poorly understood. To facilitate genetic analyses of such processes in mycobacteria, we and others have developed regulated expression systems that are repressed by a tetracycline repressor (TetR) and induced with tetracyclines, permitting the construction of conditional mutants of essential genes. A disadvantage of these systems is that tetracyclines function as transcriptional inducers and have to be removed to initiate gene silencing. Recently, reverse TetR mutants were identified that require tetracyclines as corepress

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5

Palm, Gottfried Julius, Ina Buchholz, Sebastiaan Werten, et al. "Thermodynamics, cooperativity and stability of the tetracycline repressor (TetR) upon tetracycline binding." Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1868, no. 6 (2020): 140404. http://dx.doi.org/10.1016/j.bbapap.2020.140404.

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6

Dingermann, T., H. Werner, A. Schütz, et al. "Establishment of a system for conditional gene expression using an inducible tRNA suppressor gene." Molecular and Cellular Biology 12, no. 9 (1992): 4038–45. http://dx.doi.org/10.1128/mcb.12.9.4038.

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We investigated the use of the prokaryotic tetracycline operator-repressor system as a regulatory device to control the expression of Dictyostelium discoideum tRNA genes. The tetO1 operator fragment was inserted at three different positions in front of a tRNA(Glu) (Am) suppressor gene from D. discoideum, and the tetracycline repressor gene was expressed under the control of a constitutive actin 6 promoter. The effectiveness of this approach was determined by monitoring the expression of a beta-galactosidase gene engineered to contain a stop codon that could be suppressed by the tRNA. When thes

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7

Dingermann, T., H. Werner, A. Schütz, et al. "Establishment of a system for conditional gene expression using an inducible tRNA suppressor gene." Molecular and Cellular Biology 12, no. 9 (1992): 4038–45. http://dx.doi.org/10.1128/mcb.12.9.4038-4045.1992.

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We investigated the use of the prokaryotic tetracycline operator-repressor system as a regulatory device to control the expression of Dictyostelium discoideum tRNA genes. The tetO1 operator fragment was inserted at three different positions in front of a tRNA(Glu) (Am) suppressor gene from D. discoideum, and the tetracycline repressor gene was expressed under the control of a constitutive actin 6 promoter. The effectiveness of this approach was determined by monitoring the expression of a beta-galactosidase gene engineered to contain a stop codon that could be suppressed by the tRNA. When thes

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8

Degenkolb, J., M. Takahashi, G. A. Ellestad, and W. Hillen. "Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor." Antimicrobial Agents and Chemotherapy 35, no. 8 (1991): 1591–95. http://dx.doi.org/10.1128/aac.35.8.1591.

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9

Moon, Jayoung, Jongsik Gam, Seung-Goo Lee, Young-Ger Suh, and Jeeyeon Lee. "Light-Regulated Tetracycline Binding to the Tet Repressor." Chemistry - A European Journal 20, no. 9 (2014): 2508–14. http://dx.doi.org/10.1002/chem.201304027.

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10

Kedracka-Krok, Sylwia, and Zygmunt Wasylewski. "A differential scanning calorimetry study of tetracycline repressor." European Journal of Biochemistry 270, no. 22 (2003): 4564–73. http://dx.doi.org/10.1046/j.1432-1033.2003.03856.x.

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11

Reichheld, Sean E., and Alan R. Davidson. "Two-way Interdomain Signal Transduction in Tetracycline Repressor." Journal of Molecular Biology 361, no. 2 (2006): 382–89. http://dx.doi.org/10.1016/j.jmb.2006.06.035.

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12

Ettner, Norbert, Wolfgang Hillen, and George A. Ellestad. "Enhanced site-specific cleavage of the tetracycline repressor by tetracycline complexed with iron." Journal of the American Chemical Society 115, no. 6 (1993): 2546–48. http://dx.doi.org/10.1021/ja00059a081.

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13

Chrast-Balz, J. "Bi-directional gene switching with the tetracycline repressor and a novel tetracycline antagonist." Nucleic Acids Research 24, no. 15 (1996): 2900–2904. http://dx.doi.org/10.1093/nar/24.15.2900.

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14

Bolintineanu, Dan S., Katherine Volzing, Victor Vivcharuk, Abdallah Sayyed-Ahmad, Poonam Srivastava, and Yiannis N. Kaznessis. "Investigation of Changes in Tetracycline Repressor Binding upon Mutations in the Tetracycline Operator." Journal of Chemical & Engineering Data 59, no. 10 (2014): 3167–76. http://dx.doi.org/10.1021/je500225x.

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15

Lim, Daniel A., Manfred Gossen, Chris W. Lehman, and Michael R. Botchan. "Competition for DNA Binding Sites between the Short and Long Forms of E2 Dimers Underlies Repression in Bovine Papillomavirus Type 1 DNA Replication Control." Journal of Virology 72, no. 3 (1998): 1931–40. http://dx.doi.org/10.1128/jvi.72.3.1931-1940.1998.

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ABSTRACT Papillomaviruses establish a long-term latency in vivo by maintaining their genomes as nuclear plasmids in proliferating cells. Bovine papillomavirus type 1 encodes two proteins required for viral DNA replication: the helicase E1 and the positive regulator E2. The homodimeric E2 is known to cooperatively bind to DNA with E1 to form a preinitiation complex at the origin of DNA replication. The virus also codes for two short forms of E2 that can repress viral functions when overexpressed, and at least one copy of the repressor is required for stable plasmid maintenance in transformed ce

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16

Kedracka-Krok, Sylwia, Andrzej Gorecki, Piotr Bonarek, and Zygmunt Wasylewski. "Kinetic and Thermodynamic Studies of Tet Repressor−Tetracycline Interaction†." Biochemistry 44, no. 3 (2005): 1037–46. http://dx.doi.org/10.1021/bi048548w.

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17

Saenger, Wolfram, Peter Orth, Caroline Kisker, Wolfgang Hillen, and Winfried Hinrichs. "The Tetracycline Repressor—A Paradigm for a Biological Switch." Angewandte Chemie International Edition 39, no. 12 (2000): 2042–52. http://dx.doi.org/10.1002/1521-3773(20000616)39:12<2042::aid-anie2042>3.0.co;2-c.

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18

Stiebritz, Martin T., Stefanie Wengrzik, Doris L. Klein, et al. "Computational Design of a Chain-Specific Tetracycline Repressor Heterodimer." Journal of Molecular Biology 403, no. 3 (2010): 371–85. http://dx.doi.org/10.1016/j.jmb.2010.07.055.

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19

Kim, Hong-Jin, and Ki Ho Kim. "Characterization of tTA and its functional domain in tetracycline repressor-mediated gene repression system." Archives of Pharmacal Research 21, no. 3 (1998): 320–25. http://dx.doi.org/10.1007/bf02975295.

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20

Beliakova, Maria M., Maria M. Anokhina, Vera A. Spiridonova, et al. "A direct photo-activated affinity modification of tetracycline transcription repressor protein TetR(D) with tetracycline." FEBS Letters 477, no. 3 (2000): 263–67. http://dx.doi.org/10.1016/s0014-5793(00)01728-2.

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21

Palm, Gottfried J., Thomas Lederer, Peter Orth, et al. "Specific binding of divalent metal ions to tetracycline and to the Tet repressor/tetracycline complex." JBIC Journal of Biological Inorganic Chemistry 13, no. 7 (2008): 1097–110. http://dx.doi.org/10.1007/s00775-008-0395-2.

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22

Biburger, Markus, Christian Berens, Thomas Lederer, Traudl Krec, and Wolfgang Hillen. "Intragenic Suppressors of Induction-Deficient TetR Mutants: Localization and Potential Mechanism of Action." Journal of Bacteriology 180, no. 3 (1998): 737–41. http://dx.doi.org/10.1128/jb.180.3.737-741.1998.

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ABSTRACT Eight Tn10 Tet repressor mutants with an induction-deficient phenotype and with primary mutations located at or close to the dimer interface were mutagenized and screened for inducibility in the presence of tetracycline. The second-site suppressors with wild-type-like operator binding activity that were obtained act, except for one, at a distance, suggesting that they contribute to conformational changes in the Tet repressor. Many of these long-range suppressors occur along the dimer interface, indicating that interactions between the monomers play an important role in Tet repressor i

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23

Werten, Sebastiaan, Daniela Dalm, Gottfried Julius Palm, Christopher Cornelius Grimm, and Winfried Hinrichs. "Tetracycline Repressor Allostery Does Not Depend on Divalent Metal Recognition." Biochemistry 53, no. 50 (2014): 7990–98. http://dx.doi.org/10.1021/bi5012805.

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24

Kleinschmidt, Christoph, Karlheinz Tovar, Wolfgang Hillen, and Dietmar Porschke. "Dynamics of repressor-operator recognition: Tn10-encoded tetracycline resistance control." Biochemistry 27, no. 4 (1988): 1094–104. http://dx.doi.org/10.1021/bi00404a003.

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25

Maxwell, I. H., and F. Maxwell. "Control of parvovirus DNA replication by a tetracycline-regulated repressor." Gene Therapy 6, no. 3 (1999): 309–13. http://dx.doi.org/10.1038/sj.gt.3300832.

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26

Orth, Peter, Frank Cordes, Dirk Schnappinger, Wolfgang Hillen, Wolfram Saenger, and Winfried Hinrichs. "Conformational changes of the Tet repressor induced by tetracycline trapping." Journal of Molecular Biology 279, no. 2 (1998): 439–47. http://dx.doi.org/10.1006/jmbi.1998.1775.

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27

Haberl, Florian, Harald Lanig, and Timothy Clark. "Induction of the tetracycline repressor: Characterization by molecular-dynamics simulations." Proteins: Structure, Function, and Bioinformatics 77, no. 4 (2009): 857–66. http://dx.doi.org/10.1002/prot.22505.

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28

Aleksandrov, Alexey, Linda Schuldt, Winfried Hinrichs, and Thomas Simonson. "Tetracycline-Tet Repressor Binding Specificity: Insights from Experiments and Simulations." Biophysical Journal 97, no. 10 (2009): 2829–38. http://dx.doi.org/10.1016/j.bpj.2009.08.050.

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29

Deuschle, U., W. K. Meyer, and H. J. Thiesen. "Tetracycline-reversible silencing of eukaryotic promoters." Molecular and Cellular Biology 15, no. 4 (1995): 1907–14. http://dx.doi.org/10.1128/mcb.15.4.1907.

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A tetracycline-controlled transrepressor protein has been engineered to silence transcriptional activities of eukaryotic promoters that are stably integrated into the chromatin of human cells. By fusing the KRAB domain of human Kox1 to the Tet repressor derived from Tn10 of Escherichia coli, a tetracycline-controlled hybrid protein (TetR-KRAB) was generated and constitutively expressed in HeLa cells. The TetR-KRAB protein binds to tet operator (tetO) sequences in the absence but not in the presence of tetracycline. When TetR-KRAB bound to tetO sequences upstream of the immediate-early promoter

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30

Orth, Peter, Claudia Alings, Dirk Schnappinger, Wolfram Saenger, and Winfried Hinrichs. "Crystallization and preliminary X-ray analysis of the Tet-repressor/operator complex." Acta Crystallographica Section D Biological Crystallography 54, no. 1 (1998): 99–100. http://dx.doi.org/10.1107/s0907444997007646.

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Three crystal forms of the repressor protein TetR class D in complex with the palindromic 17 bp operator sequence containing T overhangs on both sides were obtained by hanging-drop vapor-diffusion methods using PEG 4000 and PEG monomethylether 5000 as precipitants. Although the crystallization conditions were very similar, up to three different crystal forms were observed in the same drop. The space groups are monoclinic C2, P21 and hexagonal P6122. The asymmetric units of the latter two crystal forms contain one repressor–operator complex. The crystal structures of these forms were solved by

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31

Park, Ho-Jin, and Uttam L. RajBhandary. "Tetracycline-Regulated Suppression of Amber Codons in Mammalian Cells." Molecular and Cellular Biology 18, no. 8 (1998): 4418–25. http://dx.doi.org/10.1128/mcb.18.8.4418.

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ABSTRACT As an approach to inducible suppression of nonsense mutations in mammalian cells, we described recently an amber suppression system in mammalian cells dependent on coexpression of Escherichia coli glutaminyl-tRNA synthetase (GlnRS) along with the E. coli glutamine-inserting amber suppressor tRNA. Here, we report on tetracycline-regulated expression of the E. coli GlnRS gene and, thereby, tetracycline-regulated suppression of amber codons in mammalian HeLa and COS-1 cells. The E. coli GlnRS coding sequence attached to a minimal mammalian cell promoter was placed downstream of seven tan

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32

Orth, Peter, Wolfram Saenger, and Winfried Hinrichs. "Tetracycline-Chelated Mg2+Ion Initiates Helix Unwinding in Tet Repressor Induction†,‡." Biochemistry 38, no. 1 (1999): 191–98. http://dx.doi.org/10.1021/bi9816610.

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33

Saenger, Wolfram, Peter Orth, Caroline Kisker, Wolfgang Hillen, and Winfried Hinrichs. "ChemInform Abstract: The Tetracycline Repressor - A Paradigm for a Biological Switch." ChemInform 31, no. 37 (2000): no. http://dx.doi.org/10.1002/chin.200037275.

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34

Klock, G., B. Unger, C. Gatz, et al. "Heterologous repressor-operator recognition among four classes of tetracycline resistance determinants." Journal of Bacteriology 161, no. 1 (1985): 326–32. http://dx.doi.org/10.1128/jb.161.1.326-332.1985.

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35

Werten, Sebastiaan, Julia Schneider, Gottfried Julius Palm, and Winfried Hinrichs. "Modular organisation of inducer recognition and allostery in the tetracycline repressor." FEBS Journal 283, no. 11 (2016): 2102–14. http://dx.doi.org/10.1111/febs.13723.

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36

Luo, Zhao-Qing, and Stephen K. Farrand. "Cloning and Characterization of a Tetracycline Resistance Determinant Present in Agrobacterium tumefaciens C58." Journal of Bacteriology 181, no. 2 (1999): 618–26. http://dx.doi.org/10.1128/jb.181.2.618-626.1999.

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ABSTRACT Agrobacterium tumefaciens C58 and its derivatives give rise to spontaneous mutants resistant to tetracycline at a high frequency. We observed that a mutation affecting a tRNA processing function significantly affected the emergence of such mutants, suggesting that C58 contained a positively acting gene conferring resistance to tetracycline. A cosmid clone conferring resistance to tetracycline in Escherichia coli andAgrobacterium was isolated from a genomic bank of one such mutant. Subcloning, transposon mutagenesis, and DNA sequence analysis revealed that this DNA fragment contained t

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37

Breton, Marc, Evelyne Sagné, Sybille Duret, Laure Béven, Christine Citti, and Joël Renaudin. "First report of a tetracycline-inducible gene expression system for mollicutes." Microbiology 156, no. 1 (2010): 198–205. http://dx.doi.org/10.1099/mic.0.034074-0.

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Inducible promoter systems are powerful tools for studying gene function in prokaryotes but have never been shown to function in mollicutes. In this study we evaluated the efficacy of the tetracycline-inducible promoter Pxyl/tetO2 from Bacillus subtilis in controlling gene expression in two mollicutes, the plant pathogen Spiroplasma citri and the animal pathogen Mycoplasma agalactiae. An S. citri plasmid carrying the spiralin gene under the control of the xyl/tetO2 tetracycline-inducible promoter and the TetR repressor gene under the control of a constitutive spiroplasmal promoter was introduc

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38

Lancashire, John F., Tamsin D. Terry, P. J. Blackall, and Michael P. Jennings. "Plasmid-Encoded Tet B Tetracycline Resistance in Haemophilus parasuis." Antimicrobial Agents and Chemotherapy 49, no. 5 (2005): 1927–31. http://dx.doi.org/10.1128/aac.49.5.1927-1931.2005.

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ABSTRACT The complete sequence of two plasmids, pHS-Tet (5.1 kb) and pHS-Rec (9.5 kb), isolated from Haemophilus parasuis strain HS1543 has been obtained. Plasmid pHS-Tet contains four open reading frames including a tet(B) tetracycline resistance gene which unusually did not have an associated tetR repressor gene. From a total of 45 H. parasuis isolates surveyed (15 international reference strains, 15 field isolates selected for their genetic diversity, and 15 recent Australian field isolates), 2 tetracycline-resistant field isolates (HS226 and HS1859) were identified. Analysis of three addit

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39

Brown, Mindy G., Elizabeth H. Mitchell, and David L. Balkwill. "Tet 42, a Novel Tetracycline Resistance Determinant Isolated from Deep Terrestrial Subsurface Bacteria." Antimicrobial Agents and Chemotherapy 52, no. 12 (2008): 4518–21. http://dx.doi.org/10.1128/aac.00640-08.

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ABSTRACT Tet 42, a novel tetracycline resistance determinant from deep subsurface bacteria, was characterized and found to have a 30% sequence similarity to TetA(Z). The protein is a putative efflux pump that shares characteristics with previously characterized pumps, including a divergently transcribed TetR repressor, a conserved GxxSDRxGRR motif, and transmembrane domains.

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40

Hinrichs, W., C. Kisker, M. Duvel, et al. "Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance." Science 264, no. 5157 (1994): 418–20. http://dx.doi.org/10.1126/science.8153629.

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41

Kamionka, Annette, Marius Majewski, Karin Roth, Ralph Bertram, Christine Kraft, and Wolfgang Hillen. "Induction of single chain tetracycline repressor requires the binding of two inducers." Nucleic Acids Research 34, no. 14 (2006): 3834–41. http://dx.doi.org/10.1093/nar/gkl316.

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42

Lanig, Harald, Olaf G. Othersen, Frank R. Beierlein, Ute Seidel, and Timothy Clark. "Molecular Dynamics Simulations of the Tetracycline-repressor Protein: The Mechanism of Induction." Journal of Molecular Biology 359, no. 4 (2006): 1125–36. http://dx.doi.org/10.1016/j.jmb.2006.04.014.

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43

Luckner, Sylvia R., Marcus Klotzsche, Christian Berens, Wolfgang Hillen, and Yves A. Muller. "How an Agonist Peptide Mimics the Antibiotic Tetracycline to Induce Tet-Repressor." Journal of Molecular Biology 368, no. 3 (2007): 780–90. http://dx.doi.org/10.1016/j.jmb.2007.02.030.

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44

Speer, B. S., N. B. Shoemaker, and A. A. Salyers. "Bacterial resistance to tetracycline: mechanisms, transfer, and clinical significance." Clinical Microbiology Reviews 5, no. 4 (1992): 387–99. http://dx.doi.org/10.1128/cmr.5.4.387.

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Tetracycline has been a widely used antibiotic because of its low toxicity and broad spectrum of activity. However, its clinical usefulness has been declining because of the appearance of an increasing number of tetracycline-resistant isolates of clinically important bacteria. Two types of resistance mechanisms predominate: tetracycline efflux and ribosomal protection. A third mechanism of resistance, tetracycline modification, has been identified, but its clinical relevance is still unclear. For some tetracycline resistance genes, expression is regulated. In efflux genes found in gram-negativ

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45

Hop, Caroline, Vivian de Waard, Jan A. van Mourik, and Hans Pannekoek. "Lack of gradual regulation of tetracycline-controlled gene expression by the tetracyclin-repressor/VP16 transactivator (tTA) in cultured cells." FEBS Letters 405, no. 2 (1997): 167–71. http://dx.doi.org/10.1016/s0014-5793(97)00179-8.

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46

Kamionka, Annette, Ralph Bertram, and Wolfgang Hillen. "Tetracycline-Dependent Conditional Gene Knockout in Bacillus subtilis." Applied and Environmental Microbiology 71, no. 2 (2005): 728–33. http://dx.doi.org/10.1128/aem.71.2.728-733.2005.

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ABSTRACT Reversible tetracycline-dependent gene regulation allows induction of expression with the tetracycline repressor (TetR) or gene silencing with the newly developed reverse mutant revTetR. We report here the implementation of both approaches with full regulatory range in gram-positive bacteria as exemplified in Bacillus subtilis. A chromosomally located gene is controlled by one or two tet operators. The precise adjustment of regulatory windows is accomplished by adjusting tetR or revtetR expression via different promoters. The most efficient induction was 300-fold in the presence of 0.

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47

Wang, Jun, Gui-Rong Wang, Nadja B. Shoemaker, and Abigail A. Salyers. "Production of Two Proteins Encoded by theBacteroides Mobilizable Transposon NBU1 Correlates with Time-Dependent Accumulation of the Excised NBU1 Circular Form." Journal of Bacteriology 183, no. 21 (2001): 6335–43. http://dx.doi.org/10.1128/jb.183.21.6335-6343.2001.

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ABSTRACT NBU1 is a mobilizable transposon that excises from theBacteroides chromosome to form a double-stranded circular transfer intermediate. Excision is triggered by exposure of the bacteria to tetracycline. Accordingly, we expected that the expression of NBU1 genes would be induced by tetracycline. To test this hypothesis, antibodies that recognized two NBU1-encoded proteins, PrmN1 and MobN1, were used to monitor production of these proteins. PrmN1 is essential for excision, and MobN1 is essential for transfer of the excised circular form. At first, expression of the genes encoding these t

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48

Thompson, Stuart A., Elizabeth V. Maani, Angela H. Lindell, Catherine J. King, and J. Vaun McArthur. "Novel Tetracycline Resistance Determinant Isolated from an Environmental Strain of Serratia marcescens." Applied and Environmental Microbiology 73, no. 7 (2007): 2199–206. http://dx.doi.org/10.1128/aem.02511-06.

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ABSTRACT Resistances to tetracycline and mercury were identified in an environmental strain of Serratia marcescens isolated from a stream highly contaminated with heavy metals. As a step toward addressing the mechanisms of coselection of heavy metal and antibiotic resistances, the tetracycline resistance determinant was cloned in Escherichia coli. Within the cloned 13-kb segment, the tetracycline resistance locus was localized by deletion analysis and transposon mutagenesis. DNA sequence analysis of an 8.0-kb region revealed a novel gene [tetA(41)] that was predicted to encode a tetracycline e

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49

Ichijo, Hiroyuki, Naoki Yoshida, Satoru Takahashi, Junko Tanaka, and Yoshihiro Miwa. "Variant of tetracycline repressor enables us to label neurons reversibly through proteolytic regulation." Neuroscience Research 71 (September 2011): e412. http://dx.doi.org/10.1016/j.neures.2011.07.1805.

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50

Lanig, Harald, Olaf G. Othersen, Ute Seidel, Frank R. Beierlein, Thomas E. Exner, and Timothy Clark. "Structural Changes and Binding Characteristics of the Tetracycline-Repressor Binding Site on Induction." Journal of Medicinal Chemistry 49, no. 12 (2006): 3444–47. http://dx.doi.org/10.1021/jm060289g.

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