Relevant bibliographies by topics / Tetrazolium test

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  1. Journal articles

Academic literature on the topic 'Tetrazolium test'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 April 2022

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Journal articles on the topic "Tetrazolium test"

1

Grzybowski, Camila Ribeiro de Souza, Osvaldo de Castro Ohlson, Rosemeire Carvalho da Silva, and Maristela Panobianco. "Viability of barley seeds by the tetrazolium test." Revista Brasileira de Sementes 34, no. 1 (2012): 47–54. http://dx.doi.org/10.1590/s0101-31222012000100006.

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The tetrazolium test is used to control seed quality of various plant species since it allows a rapid evaluation of viability. Freshly harvested barley seeds show dormancy that can make the germination test ineffective for an immediate evaluation. Therefore, the development of more efficient methods, such as the tetrazolium test, is necessary. The objective of this research work was to study various procedures for performing the tetrazolium test on barley seeds. Five lots of cv. BRS 195 barley seeds were used and subjected to the following treatments: two different methods of seed preconditioning (direct immersion in H2O and between sheets of moistened paper towels); two types of preparation for staining (longitudinal cross-section of the seed through the embryo with immersion of one half in a 2,3,5 triphenyl tetrazolium chloride solution or placing both halves on top of filter paper moistened with the tetrazolium salt solution); two methods of staining (on top of filter paper and direct immersion in the tetrazolium salt solution). Three concentrations of the tetrazolium salt solution (0.1%, 0.5%, and 1.0%) were used. It was concluded that the tetrazolium test on barley seeds may be accomplished with preconditioning by direct immersion in H2O and staining by immersing in a 0.1% or 0.5% concentration of tetrazolium salt solution or staining on top of filter paper moistened with such solution at a 1.0% concentration.
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2

Carvalho, Tereza Cristina de, Francisco Carlos Krzyzanowski, Osvaldo de Castro Ohlson, and Maristela Panobianco. "Tetrazolium test adjustment for wheat seeds." Journal of Seed Science 35, no. 3 (2013): 361–67. http://dx.doi.org/10.1590/s2317-15372013000300013.

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The assessment of the germination test in wheat seeds varies from 4 to 15 days, because the species normally presents dormancy in freshly harvested seeds. The tetrazolium test can characterize seed viability in less than 24 hours including lots with dormancy seeds. The objective of this study was to develop a practical and efficient procedure for evaluating the viability of wheat seeds using the tetrazolium test. Five seed lots of the BRS 208 cultivar were used, where the following were tested: a) pre-conditioning between moist paper towels or direct immersion in water for 18 hours, at 20 °C; b) longitudinal section of the embryo and the endosperm; c) coloration on paper or by immersion for 2 and 3 hours, at 30 and 40 °C; and d) concentrations of tetrazolium solution at 0.075%, 0.1%, 0.5% and 1.0%. The tetrazolium test may be efficiently used to evaluate wheat seed viability by pre-conditioning the seeds between paper towels (18 hours, at 20 °C) and adopting the following combinations of preparation and coloration: coloration of both halves of the seed on paper (2 hours, at 30 °C), in a 1.0% tetrazolium solution; or coloration of one half of the seed by immersion (3 hours, at 30 °C), in a 0.1% tetrazolium solution; or coloration of one half of the seed by immersion (2 hours, at 40 °C), in a 0.075% tetrazolium solution. This latter procedure is recommended for identifying and discarding lots with lower viability.
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3

Fantazzini, Tatiana Botelho, Sttela Dellyzete Veiga Franco da Rosa, Gladyston Rodrigues Carvalho, et al. "Correlation between historical data of the germination test and of the tetrazolium test in coffee seeds by GAMLSS." Seed Science and Technology 48, no. 2 (2020): 179–88. http://dx.doi.org/10.15258/sst.2020.48.2.05.

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In the Brazilian National System of Seeds and Seedlings, coffee seeds are evaluated by the germination or tetrazolium test. However, differences have been observed between the results of these tests in various studies, especially when the seeds have a lower level of quality. Given this situation, the aim of this study was to evaluate the correlation between historical data of results of the germination test and of the tetrazolium test in samples of coffee seeds using Generalized Additive Models for Location, Scale and Shape (GAMLSS). Historical data of results of the germination test and of the tetrazolium test of coffee seeds originating from different cultivars and different crop seasons were used. The zero-or-one inflated beta GAMLSS is suitable for fitting data from the germination test and from the tetrazolium test. The estimate of viability by the tetrazolium test varies according to the germination percentage class. There are greater GAMLSS correlations between the percentages of normal seedlings and of viability in the tetrazolium test for germination values above 70%, and low correlations below this value, showing that evaluation of coffee seeds based only on the tetrazolium test may not correspond to actual physiological performance.
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4

Carvalho, Ireni Leitzke, Géri Eduardo Meneghello, Lilian Madruga de Tunes, Caroline Costa Jácome, and Vanessa Nogueira Soares. "Methodological adjustments to the tetrazolium test in rice seeds." Journal of Seed Science 39, no. 1 (2017): 41–49. http://dx.doi.org/10.1590/2317-1545v39n1169643.

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Abstract: Reducing the execution time of the tetrazolium test is important because it is used for making decisions during the preharvest and this test takes approximately 24 h. Thus, the goal of this research was to study preconditioning and staining periods and concentration of tetrazolium salt, in order to reduce the evaluation time of rice seed viability by tetrazolium test. Three independent experiments were conducted. In the first and second experiments, six rice seed lots from the BR Irga 424 cultivar were used. In the first experiment, different concentrations of tetrazolium salt (0.1, 0.25, 0.5 and 1 %) and staining times (0.5, 1.0 and 2.0 h) were evaluated. In the second one, different hydration periods (0.5, 1.0, 2.0 and 4.0 h) at two temperatures (35 and 40 °C) were tested. In the third one, eight seed lots from the same cultivar were used, and the effectiveness of the modified tetrazolium test in the evaluation of rice seed viability was assesed. It is possible to carried out the tetrazolium test on rice using the hydration of peeled seeds for 1 h at 40 °C and staining for 1 h with a 0.25% salt concentration.
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5

Santos, M. A. O., A. D. L. C. Novembre, and J. Marcos-Filho. "Tetrazolium test to assess viability and vigour of tomato seeds." Seed Science and Technology 35, no. 1 (2007): 213–23. http://dx.doi.org/10.15258/sst.2007.35.1.19.

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6

Cordeiro, Luciana Nunes, Riselane de Lucena Alcântara Bruno, Lucas Kennedy Silva Lima, Albericio Pereira de Andrade, Karialane da Silva Belarmino, and Edna Ursulino Alves. "Suitability of tetrazolium test for Tamarindus indica L. seeds." Semina: Ciências Agrárias 43, no. 2 (2022): 509–22. http://dx.doi.org/10.5433/1679-0359.2022v43n2p509.

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Seed quality is routinely assessed by direct tests, e.g, the germination test, or indirect tests like the tetrazolium test, which has shoown to be promising in the determine viability and vigor, allowing the diagnosis of the main problems that may affect seed quality, such as mechanic damages, field deterioration and storage. In this respect, this study was conducted to develop a tetrazolium test protocol to evaluate the viability and vigor of Tamarindus indica L. seeds. Before exposing the seeds to the tetrazolium solution, seed preconditioning studies were carried out in which seven soaking times were tested. The soaking time that did not cause damage to the seed embryo and allowed the removal of the seed coat to expose the seed structures to the tetrazolium salt was selected. Then, an experiment was set up in a completely randomized design with a 2x3x3 factorial arrangement involving two seed lots, three soaking times in tetrazolium salt (6, 12 and 16 h) and three salt concentrations (0.075, 0.1 and 0.5%), totaling 18 treatments with four replicates of 25 seeds, evaluated at 40 ºC. For each treatment, the seeds were divided into three classes, namely, viable and vigorous embryos (class 1); viable embryos (class 2) and non-viable or dead embryos (class 3). For a comparison with the tetrazolium test results, the two seed lots were analyzed for water content, germination, emergence, first count, germination speed index, emergence speed index, growth and seedling dry weight. The viability and vigor of T. indica seeds can be evaluated after a soaking period of 48 h and immersion for 6h in tetrazolium salt at the concentration of 0.1%, at 40°C, with provides results similar to conventional seed viability tests. The tetrazolium test proved to be adequate to differentiate T. indica seed lots in terms of viability.
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7

BRITO, WASHINGTON APARECIDO DA LUZ, KLEANE TARGINO OLIVEIRA PEREIRA, NARJARA WALESSA NOGUEIRA, SALVADOR BARROS TORRES, and EMANOELA PEREIRA DE PAIVA. "EVALUATION OF VIABILITY OF Tabebuia aurea SEEDS THROUGH TETRAZOLIUM TEST." Revista Caatinga 33, no. 4 (2020): 993–99. http://dx.doi.org/10.1590/1983-21252020v33n414rc.

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ABSTRACT The viability of T. aurea seeds is evaluated through the germination test, taking 21 days, which makes it difficult to obtain quick information about the viability of its seeds. In this context, using the tetrazolium test as an evaluation method would be appropriate because it provides faster and more reliable information for several species. In view of the above, the objective of this study was to adapt the tetrazolium test methodology to evaluate the viability of T. aurea seeds. Initially, the imbibition curve was constructed to determine the appropriate hydration period for the seeds and the germination test was conducted, both for evaluating the initial quality and for comparison with the tetrazolium test results. For the tetrazolium test, the seeds were hydrated for 24 hours, then their coats were removed and the seeds were immersed in tetrazolium solutions. The concentrations used were 0.05, 0.075 and 0.1% for three staining periods 2, 4 and 6 hours, at 35 and 40 °C, in the absence of light. The experimental design was completely randomized in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium solution x three staining periods + one control = germination test) with four replicates of 25 seeds. Tetrazolium test was adequate to evaluate the viability of T. aurea seeds using the concentration of 0.05% for four hours at 40 °C.
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8

Souza, Antônia Adailha Torres, Narjara Walessa Nogueira, Salvador Barros Torres, Clarisse Pereira Benedito, Maria Lilia de Souza Neta, and Emanoela Pereira de Paiva. "TETRAZOLIUM TEST TO ESTIMATE OKRA (Abelmoschus esculentus (L.) MOENCH) SEED VIABILITY." Revista Caatinga 31, no. 4 (2018): 817–22. http://dx.doi.org/10.1590/1983-21252018v31n403rc.

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ABSTRACT This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an 18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.
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9

Souza, Camila Ribeiro de, Osvaldo de Castro Ohlson, Melícia Ingredi Araújo Gavazza, and Maristela Panobianco. "Tetrazolium test for evaluating triticale seed viability." Revista Brasileira de Sementes 32, no. 3 (2010): 163–69. http://dx.doi.org/10.1590/s0101-31222010000300018.

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Freshly harvested triticale seeds are usually dormant, making the immediate evaluation of the physiological potential of seed lots difficult. We evaluated different triphenyl tetrazolium chloride (TTC) test methods for rapidly determining the viability of four seed lots of x.Triticosecale Wittmack cultivar IPR111. The test variables were: Preconditioning, (i) placing whole seeds between moistened paper towels or (ii) directly soaking the seeds in water, both procedures being conducted at 20 ºC for 18 hours; Post-conditioning seed preparation, (i) longitudinal bisection of the seed through the embryo with one half being stained and the other discarded or (ii) longitudinal bisection with both halves being stained; Staining for three and four hours, in the dark, with 0.1%, 0.5% or 1.0% (w/v) TTC according to the preconditioning method described above, (i) both halves of each seed were placed on filter paper moistened with TTC and maintained at 40 ºC or (ii) one half of each seed was immersed in 5 mL of TTC solution in a 100 mL glass beaker at 30 ºC. The best results were obtained by preconditioning seeds between moistened paper towels at 20 ºC for 18 hours and staining on filter paper with 1.0% (w/v) TTC for three hours at 40 ºC.
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10

Arrowsmith, W. A. "THE NITROBLUE TETRAZOLIUM (NBT) TEST AND MENINGITIS." Developmental Medicine & Child Neurology 15, no. 4 (2008): 519–21. http://dx.doi.org/10.1111/j.1469-8749.1973.tb05076.x.

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