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Journal articles on the topic 'Thermophilic bacteria'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Uemura, Naoki A., and Daisuke Nakane. "Type IV Pili in Thermophilic Bacteria: Mechanisms and Ecological Implications." Biomolecules 15, no. 4 (2025): 459. https://doi.org/10.3390/biom15040459.

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Type IV pili (T4P) machinery is critical for bacterial surface motility, protein secretion, and DNA uptake. This review highlights the ecological significance of T4P-dependent motility in Thermus thermophilus, a thermophilic bacterium isolated from hot springs. Unlike swimming motility, the T4P machinery enables bacteria to move over two-dimensional surfaces through repeated cycles of extension and retraction of pilus filaments. Notably, T. thermophilus exhibits upstream-directed migration under shear stress, known as rheotaxis, which appears to represent an adaptive strategy unique to thermop

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2

Allgood, Gregory S., and Jerome J. Perry. "Oxygen defense systems in obligately thermophilic bacteria." Canadian Journal of Microbiology 31, no. 11 (1985): 1006–10. http://dx.doi.org/10.1139/m85-190.

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Ten strains of Gram-negative, aerobic, obligately thermophilic bacteria were examined for their response to oxygen toxicity by comparing static with shaken cultures. All of the organisms tested had measurable levels of superoxide dismutase, catalase, and peroxidase. Aeration generally did not result in an increased level of superoxide dismutase in any of the thermophiles. Aeration of organisms obligate for n-alkane substrate caused an increase in cellular peroxidase levels and a corresponding decrease in catalase. The thermophiles that grew on either n-alkanes or complex media did not grow on

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3

Kim and Lee. "Effects of a Groundwater Heat Pump on Thermophilic Bacteria Activity." Water 11, no. 10 (2019): 2084. http://dx.doi.org/10.3390/w11102084.

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Groundwater samples were collected from the tubular wells of a groundwater heat pump (GWHP), and the psychrophilic, mesophilic, and thermophilic bacteria inhabiting the collected groundwater were cultured and isolated. Using the isolated bacteria, we analyzed temperature-dependent changes in autochthonous bacteria based on the operation of the GWHP. Microbial culture identified eight species of bacteria: five species of thermophilic bacteria (Anoxybacillus tepidamans, Bacillus oceanisediminis, Deinococcus geothermalis, Effusibacillus pohliae, and Vulcaniibacterium thermophilum), one species of

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4

Safitri, Ratu, Rika Okta Pylia, and Shabarni Gaffar. "Amylolytic Geobacillus from Kamojang Crater Hot Springs, Garut, Indonesia." Research Journal of Chemistry and Environment 26, no. 10 (2022): 62–69. http://dx.doi.org/10.25303/2610rjce062069.

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The hot springs area of Kamojang Crater, Garut Indonesia is a prospective habitat for amylase-producing thermophile bacteria. Researchers have been drawn to thermophilic bacteria because they produce thermostable enzymes that can be used in biotechnological processes. Thermostable enzyme produced by thermophilic bacteria is used extensively in industrial processes. This study aims to isolate and characterize the amylolytic thermophilic bacteria and archaea from Kamojang Crater hot springs, Garut, Indonesia. Samples were grown in Thermus, Luria Bertani and hot spring medium at 70°C and pH 6, ch

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Vavitsas, Konstantinos, Panayiotis D. Glekas, and Dimitris G. Hatzinikolaou. "Synthetic Biology of Thermophiles: Taking Bioengineering to the Extremes?" Applied Microbiology 2, no. 1 (2022): 165–74. http://dx.doi.org/10.3390/applmicrobiol2010011.

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Synthetic biology applications rely on a well-characterized set of microbial strains, with an established toolbox of molecular biology methods for their genetic manipulation. Since there are no thermophiles with such attributes, most biotechnology and synthetic biology studies use organisms that grow in the mesophilic temperature range. As a result, thermophiles, a heterogenous group of microbes that thrive at high (>50 °C) temperatures, are largely overlooked, with respect to their biotechnological potential, even though they share several favorable traits. Thermophilic bacteria tend to gr

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6

KHALILA, RUHUL, Lenni Fitri, and SUHARTONO SUHARTONO. "Isolation and Characterization of Thermophilic Bacteria as Cellulolytic Enzyme Producer from the Hot Spring of Ie Seuum Aceh Besar, Indonesia." Microbiology Indonesia 14, no. 1 (2020): 4. http://dx.doi.org/10.5454/mi.14.1.4.

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Cellulase enzymes can be isolated from thermophile bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to recover and characterize the isolates morphologically and biochemically followed by determination of the thermophile bacterial isolates potential as cellulolytic enzyme producers,. The sampling method in this research was conducted by a purposive sampling at temperature of 70 oC, 60 oC and 50 oC. Isolation of thermophilic bacteria was carried out on nutrient agar (NA) media. There were four isolates of thermophilic bacteria isolated recovered at 70 oC, five isol

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7

Mawati, Sefi Desfeni, Esti Harpen, and Hilma Putri Fidyandini. "SKRINING BAKTERI TERMOFILIK POTENSIAL AMILOLITIK DARI SUMBER AIR PANAS WAY BELERANG KALIANDA LAMPUNG SELATAN." Journal of Aquatropica Asia 6, no. 1 (2021): 1–7. http://dx.doi.org/10.33019/aquatropica.v6i1.2458.

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Thermophilic bacteria that produced amylase and protease have been isolated from Way Belerang hot spring, Kalianda, South Lampung. This research aims to screen and identify thermophilic bacteria that have the potential to produce thermostable amylase and protease enzymes.The research procedures included sampling, isolation of enzyme-producing thermophilic bacteria, a series of phenotypic and biochemical tests, and molecular identification by 16s rRNA. This study used 2 treatments, namely incubation temperature 37 and 50 ºC with 3 repetitions. The results showed that the optimum temperature for

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8

Jasilionis, Andrius, Algirdas Kaupinis, Marija Ger, Mindaugas Valius, Donaldas Chitavichius, and Nomeda Kuisiene. "Gene expression and activity analysis of the first thermophilic U32 peptidase." Open Life Sciences 7, no. 4 (2012): 587–95. http://dx.doi.org/10.2478/s11535-012-0047-y.

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AbstractPeptidase family U32 is one of the few whose catalytic type and structure has not yet been described. It is generally accepted that U32 peptidases represent putative collagenases and contribute to the pathogenicity of some bacteria. Meanwhile, U32 peptidases are also found in nonpathogenic bacteria including thermophiles and hyperthermophiles. Here we report cloning of the U32.002 peptidase gene from thermophilic Geobacillus thermoleovorans DSM 15325 and demonstrate expression and characterization of the recombinant protein. It has been determined that U32.002 peptidase is constitutive

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9

Ebrahimpour, Arezoo, and Ashraf Kariminik. "Isolation, characterization and molecular identification of protease producing bacteria from Tashkooh mountain located in Ahvaz, Iran." International Journal of Life Sciences 9, no. 2 (2015): 39–42. http://dx.doi.org/10.3126/ijls.v9i2.12054.

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Thermophilic microorganisms have gained worldwide importance due to their remarkable potential to produce thermostable and thermoactive enzymes that have wide applications in pharmaceuticals and industries. Therefore, the isolation of thermophilic bacteria from natural sources and their identification are very important in terms of discovering new industrial enzymes. The aim of this research was therefore the isolation of protease producing thermophilic bacteria from Tashkooh or firing mount located in Ahvaz, Iran. 8 bacterial isolates were screened. These strains were examined for the existen

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10

Yoshida, Naoto. "Simple Assessing of Calcification Catalyzed by Thermophilic Bacteria." European Journal of Theoretical and Applied Sciences 1, no. 5 (2023): 786–89. http://dx.doi.org/10.59324/ejtas.2023.1(5).65.

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Several bacterial species have been found to involve the biomineralization phenomenon promoted by them. The formation of calcium carbonate mineral (calcification) is most abundant phenomena as biomineralization by bacteria. Here we introduce the simple assessing method of calcification catalyzed by thermophilic bacteria. To obtain the fresh biomass of thermophilic bacterium, the thermophilic bacteria was cultured on conventional nutrient agar medium at 60˚C. Fresh biomass of bacteria is simply placed on calcite promoting hydrogel surface, and incubated at 60˚C. After incubation for 24 to 72 h,

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11

Naoto, Yoshida. "Simple Assessing of Calcification Catalyzed by Thermophilic Bacteria." European Jornal of Theoretical and Sciences 1, no. 5 (2023): 786–89. https://doi.org/10.59324/ejtas.2023.1(5).65.

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Several bacterial species have been found to involve the biomineralization phenomenon promoted by them. The formation of calcium carbonate mineral (calcification) is most abundant phenomena as biomineralization by bacteria. Here we introduce the simple assessing method of calcification catalyzed by thermophilic bacteria. To obtain the fresh biomass of thermophilic bacterium, the thermophilic bacteria was cultured on conventional nutrient agar medium at 60˚C. Fresh biomass of bacteria is simply placed on calcite promoting hydrogel surface, and incubated at 60˚C. After incubation for 24 to 72 h,

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12

Ifandi, Slamet, and Muh Alwi. "Isolation of Thermophilic Bacteria from Bora Hot Springs in Central Sulawesi." Biosaintifika: Journal of Biology & Biology Education 10, no. 2 (2018): 291–97. http://dx.doi.org/10.15294/biosaintifika.v10i2.14905.

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Thermophilic bacteria can survive at high temperature, in which hot spring is one of its habitats. Indonesia has many hot springs with potential as a habitat for thermophilic bacteria. The purpose of this study was to obtain isolates thermophilic bacteria from Bora hotspring located in Central Sulawesi. This study applied a descriptive-observational study design, characteristics of bacterial properties identified using conventional methods according to the Bergey’s Manual of Determinative Bacteriology. The study was conducted in 3 stages. The first stage was bacteria cultivation on the appropr

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13

Korehi, Hananeh, and Axel Schippers. "Bioleaching of a Marine Hydrothermal Sulfide Ore with Mesophiles, Moderate Thermophiles and Thermophiles." Advanced Materials Research 825 (October 2013): 229–32. http://dx.doi.org/10.4028/www.scientific.net/amr.825.229.

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Marine hydrothermal polymetallic sulfide ores contain high amounts of valuable metals such as Cu, Pb, Zn, Au, Ag, as well as In, Ge, Bi, and Se. Samples from a site in the Indian Ocean were taken during a BGR ship cruise, crushed and sieved for bioleaching experiments to reveal the extraction of the various metals. Chalcopyrite was the main mineral, the total copper content was 38.5 %wt. Comparative bioleaching with mesophilic, moderate thermophilic and thermophilic acidophilic iron- and sulfur-oxidizing bacteria and archaea was investigated. Batch culture experiments were conducted at 2% (w/v

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14

Trunilina, M. V., A. A. Vologzhannikova, T. A. Kudryashov, et al. "Physical and Biochemical Properties of N-Acetyl Transferase RimL of the Hyperthermophilic Bacteria Thermus thermophilus." Journal Biomed 20, no. 3 (2024): 47–51. http://dx.doi.org/10.33647/2074-5982-20-3-47-51.

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Bacterial N-terminal acetyltransferases (NATs) are involved in the biosynthesis/degradation of antibiotics. The RimL enzyme from E. coli provides it with resistance to the antibiotic microcin C. To date, the NATs of pathogenic bacteria have been well studied, but there is no data on the NATs of thermophilic bacteria. The purpose of the work is to study the physicochemical properties and specificity of a new NAT — RimL from Thermus thermophilus. We cloned the RimL ORF (TTHA1799) and developed a method for purifying the enzyme. The stability of RimL to pH, high temperatures and denaturing agents

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15

Mikulík, Karel, Magdalena Melčová, and Jarmila Zídková. "Antibacterial peptides from thermophilic bacteria." International Journal of Engineering Research and Science 3, no. 5 (2017): 46–57. http://dx.doi.org/10.25125/engineering-journal-ijoer-apr-2017-15.

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16

Versiska, Poppy Mai, Minda Azhar, Bahrizal, Toyokazu Yoshida, and Mitsukura Koichi. "Molecular Identification of Inulin-Degrading Thermophiles: A 16S rRNA Gene-Based Study." BIO Web of Conferences 163 (2025): 01009. https://doi.org/10.1051/bioconf/202516301009.

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Thermophilic bacteria are a potential source of thermostable enzymes capable of degrading inulin. One prominent habitat for these bacteria is hot springs. Despite their significance, many thermophilic bacteria remain unidentified at the molecular level. This study conducted to identify inulin-degrading thermophilic bacteria from the Sonsang hot spring in Solok, West Sumatera, using 16S rRNA gene analysis. Chromosomal DNA was extracted from the bacterial isolate, and the 16S rRNA gene was amplified through Polymerase Chain Reaction. The amplified gene products were purified, visualized using ag

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17

Irdawati, Irdawati, Fahra Fahra, Dwi Hilda Putri, Dezi Handayani, and Yusrizal Yusrizal. "Effect of the Thermophilic Bacterial Biculture Consortium from Mudiak Sapan Hot Springs on Biofuel Production." Jurnal Penelitian Pendidikan IPA 9, no. 10 (2023): 9032–37. http://dx.doi.org/10.29303/jppipa.v9i10.3597.

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Biofuels are fuels derived from biomass and consist of biogas, biodiesel and bioethanol. Bioethanol is a biofuel whose main ingredients are from plants and generally use microorganisms in the fermentation process. One way is to use thermophilic bacteria with the advantage of low contamination levels and better product quality. Bacteria that exist in nature are not only in a single form but also exist in the form of a consortium in which there are beneficial or detrimental bacterial interactions. Favorable bacterial interactions indicate that the bacteria are compatible. Using a consortium with

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18

Mohanta, Subham, Megha Bahuguna, John David Baley, Shivika Sharma, and Vikas Sharma. "Extremophilic Cellulases: A Comprehensive Review." Journal of Tropical Biodiversity and Biotechnology 8, no. 3 (2023): 74986. http://dx.doi.org/10.22146/jtbb.74986.

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Microbial cellulases are an important industrial enzyme having diverse applications in biotechnology, environmental challenges, industrial products and processes. Extremophiles like thermophillic bacteria are a good source of industrially important cellulases as these can withstand industrially rigorous procedures like paper deinking, fabric material softening, bio stoning, paper and pulp, biopolishing cloth material, animal feed and juice. Identification of novel cellulases or improving them through biotechnological interventions has remained a challenge for researchers. Genetic manipulation

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19

COGAN, TIMOTHY M., MANUELA BARBOSA, ERIC BEUVIER, et al. "Characterization of the lactic acid bacteria in artisanal dairy products." Journal of Dairy Research 64, no. 3 (1997): 409–21. http://dx.doi.org/10.1017/s0022029997002185.

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In all, 4379 isolates from 35 products, including 24 artisanal cheeses, were surveyed with a view to identifying strains that could be used as starters in commercial dairy fermentations. Of the isolates, 38% were classified as Lactococcus, 17% as Enterococcus, 14% as Streptococcus thermophilus, 12% as mesophilic Lactobacillus, 10% as Leuconostoc and 9% as thermophilic Lactobacillus. Acid production by the isolates varied considerably. Of the 1582 isolates of Lactococcus and 482 isolates of mesophilic Lactobacillus tested, only 8 and 2% respectively produced sufficient acid to lower the pH of m

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20

Çulha, Mustafa, Ahmet Adigüzel, M. MÜGE Yazici, Mehmet Kahraman, Fikrettin Slahin, and Medine Güllüce. "Characterization of Thermophilic Bacteria Using Surface-Enhanced Raman Scattering." Applied Spectroscopy 62, no. 11 (2008): 1226–32. http://dx.doi.org/10.1366/000370208786401545.

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Surface-enhanced Raman scattering (SERS) can provide molecular-level information about the molecules and molecular structures in the vicinity of nanostructured noble metal surfaces such as gold and silver. The three thermophilic bacteria Bacillus licheniformis, Geobacillus stearothermophilus, and Geobacillus pallidus, a Gram-negative bacterium E. coli, and a Gram-positive bacterium B. megaterium are comparatively characterized using SERS. The SERS spectra of thermophilic bacteria are similar, while they show significant differences compared to E. coli and B. megaterium. The findings indicate t

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Abed, Hayder H., Aamer M. Ali, and Zahrah S. Mahdi. "Effect of Vitamin D3 Supplement on the Semen Quality in Human Patients with Vitamin D Deficiency." HAYATI Journal of Biosciences 29, no. 5 (2022): 562–69. http://dx.doi.org/10.4308/hjb.29.5.562-569.

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Thermophilic bacteria are a source of bioactive compounds that have many benefits for human life. One of them is as a source of antimicrobials. This research aimed to identify and characterize the promising thermophilic bacterial isolates by analyzing bioactive compounds and their potential as antimicrobial agents. Thermophilic bacteria with the code LBKURCC were taken from the collection of the Biochemistry Laboratory of the University of Riau. Forty-four purified strains of thermophilic bacteria were tested for antimicrobial ability. These thermophilic bacteria were taken from hot springs lo

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Octarya, Zona, Titania Tjandrawati Nugroho, Yuana Nurulita, and Saryono. "Molecular Identification, GC-MS Analysis of Bioactive Compounds and Antimicrobial Activity of Thermophilic Bacteria Derived from West Sumatra Hot-Spring Indonesia." HAYATI Journal of Biosciences 29, no. 4 (2022): 549–61. http://dx.doi.org/10.4308/hjb.29.4.549-561.

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Thermophilic bacteria are a source of bioactive compounds that have many benefits for human life. One of them is as a source of antimicrobials. This research aimed to identify and characterize the promising thermophilic bacterial isolates by analyzing bioactive compounds and their potential as antimicrobial agents. Thermophilic bacteria with the code LBKURCC were taken from the collection of the Biochemistry Laboratory of the University of Riau. Forty-four purified strains of thermophilic bacteria were tested for antimicrobial ability. These thermophilic bacteria were taken from hot springs lo

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23

Wang, Chuan, Yuze Yang, Shaoqing Tang, et al. "Comparison of Structural Features of CRISPR-Cas Systems in Thermophilic Bacteria." Microorganisms 11, no. 9 (2023): 2275. http://dx.doi.org/10.3390/microorganisms11092275.

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The clustered regularly interspaced short palindromic repeat (CRISPR) is an adaptive immune system that defends most archaea and many bacteria from foreign DNA, such as phages, viruses, and plasmids. The link between the CRISPR-Cas system and the optimum growth temperature of thermophilic bacteria remains unclear. To investigate the relationship between the structural characteristics, diversity, and distribution properties of the CRISPR-Cas system and the optimum growth temperature in thermophilic bacteria, genomes of 61 species of thermophilic bacteria with complete genome sequences were down

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Megahati, Ruth Rize Paas. "Identification of Amylase-Producing Thermophilic Bacteria through 16sRNA Gene Analysis: MGH3 Strain." Jurnal Penelitian Pendidikan IPA 9, no. 2 (2023): 621–25. http://dx.doi.org/10.29303/jppipa.v9i2.3080.

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Thermostable amylase plays a role in the industrial sector because it is resistant to high temperatures, has high enzyme activity in various parameters and is economical in production costs. The thermostable amylase used in industry is stable and thermo activity. Bacteria that produce thermostable amylase can come from hot springs with temperatures above 80°C, such as Semurup hot springs, Kerinci, Jambi province, Indonesia. Semurup hot spring (Jambi) has a temperature of 82°C and a pH of 8.6. This causes variations in the content of organic, inorganic, and mineral compounds in the Semurup hot

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Adhikari, Hriush, Sangam Ghimire, Binod Khatri, and Yuvraj K.C. "Enzymatic Screening and Molecular Characterization of Thermophilic Bacterial Strains Isolated from Hotspring of Tatopani, Bhurung, Nepal." International Journal of Applied Sciences and Biotechnology 3, no. 3 (2015): 392–97. http://dx.doi.org/10.3126/ijasbt.v3i3.12724.

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Background and Aim: In Nepal not much of study of Thermophilic area and Thermophiles have been done. Thermophilic bacteria are less studied but are important group of microorganisms due to their ability to produce industrially important enzymes. Methods: In this study, thermophilic bacteria were isolated from hot spring of Bhurung, Nepal. Wide range of bacteria that could grow at high temperatures and tolerate extreme temperature were characterized by morphology, biochemistry and sequencing of its 16S rRNA gene sequence. The isolates were screened for production of extracellular enzymes like p

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Suharti, Nin Suharti, and Geminsah Putra. "ISOLASI DAN IDENTIFIKASI BAKTERI TERMOFILIK PENGHASIL AMILASE DARI SUMBER AIR PANAS SIDEBUK-DEBUK SUMATERA UTARA." Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) 17, no. 1 (2022): 147–57. http://dx.doi.org/10.36911/pannmed.v17i1.1277.

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Hot springs or hot springs are springs that are produced as a result of the release of groundwater from the earth's crust after experiencing geothermal heating. Indonesia is one of the most active tectonic regions in the world with more than 70 active volcanoes, and has many geothermal areas. Hot springs are a suitable growth medium for thermophilic bacteria. Thermophilic bacteria are a group of bacteria that adapt to high-temperature environmental conditions, with temperatures ranging from 50°C-75°C. The natural habitat of thermophilic bacteria is widespread throughout the earth's surface, in

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Mahestri, Laras, Esti Harpeni, and Agus Setyawan. "Isolation and Screening of Amylolytic and Proteolytic Thermophilic Bacteria from Way Panas Hot Spring, Kalianda, South Lampung." Jurnal Perikanan dan Kelautan 26, no. 3 (2021): 161. http://dx.doi.org/10.31258/jpk.26.3.161-168.

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Amylolytic and proteolitic activity from thermophilic bacteria have been observed from the Way Panas hot springs, Kalianda, South Lampung. This study aims to isolate and screen amylase and protease enzyme-producing thermophilic bacteria and their potential in fisheries. A total of 8 bacterial isolates were examined from the Way Panas hot spring, Kalianda. Each was cultured on selective medium for amylase and protease production. The results showed that 4 bacterial isolates were able to hydrolyze starch (A.WP.50.1, A.WP.50.1, A.WP.50.3, and A.WP.50.4) were able hydrolyze starch and no bacterial

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Hamana, Koei, Teruhiko Akiba, Fuji Uchino, and Shigeru Matsuzaki. "Distribution of spermine in bacilli and lactic acid bacteria." Canadian Journal of Microbiology 35, no. 4 (1989): 450–55. http://dx.doi.org/10.1139/m89-069.

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Obligate moderately thermophilic bacilli and obligate moderately thermoacidophilic bacilli contained spermine as the major polyamine in addition to putrescine and spermidine. The identity of spermine was confirmed by thin-layer chromatography and high-performance liquid chromatography before and after treatment with putrescine oxidase. Using these methods, thermospermine and spermine can be separated; thermospermine was not present in these organisms. On the other hand, various facultative thermophiles and mesophilic strains of the genus Bacillus, including alkalophiles and halophiles, lack sp

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Gladden, John M., Martin Allgaier, Christopher S. Miller, et al. "Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass." Applied and Environmental Microbiology 77, no. 16 (2011): 5804–12. http://dx.doi.org/10.1128/aem.00032-11.

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ABSTRACTIndustrial-scale biofuel production requires robust enzymatic cocktails to produce fermentable sugars from lignocellulosic biomass. Thermophilic bacterial consortia are a potential source of cellulases and hemicellulases adapted to harsher reaction conditions than commercial fungal enzymes. Compost-derived microbial consortia were adapted to switchgrass at 60°C to develop thermophilic biomass-degrading consortia for detailed studies. Microbial community analysis using small-subunit rRNA gene amplicon pyrosequencing and short-read metagenomic sequencing demonstrated that thermophilic ad

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Herwanda, Fadia Rezika, and Tetty Marta Linda. "Isolation and Characterization of Thermophilic Bacteria as Producing Enzyme Amylase from Kepanasan Hot Springs In Tapung Hulu, Kampar, Riau." Jurnal Biologi Tropis 25, no. 1 (2025): 967–74. https://doi.org/10.29303/jbt.v25i1.7888.

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Thermophilic bacteria are a group of bacteria that can live and survive at high temperatures ranging from 45°C-80°C. Thermophilic bacteria are the best choice as a producer of thermostable enzymes that can endure high temperatures used in various industrial fields including the textile industry, food industry, detergent industry, and pharmaceutical industry. This study aims to isolate, characterize, and test the activity of thermostable amylase enzyme from thermophilic bacterial isolates in Kepanasan hot spring in Tapung Hulu District, Kampar Regency, Riau. Characterization of thermophilic bac

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Godon, Jean Jacques, Amandine Galès, Eric Latrille, Pornpimol Ouichanpagdee, and Jean-Philippe Seyer. "An "overlooked" habitat for thermophilic bacteria: the phyllosphere." BioDiscovery 23 (March 30, 2020): e47033. https://doi.org/10.3897/biodiscovery.23.e47033.

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Thermophilic microbes are present everywhere around us and their only known natural biotope is far away and most usually associated with geothermal energy. To answer this paradox, we explore the hypothesis that the phyllosphere (surface of leaves), due to its exposition to the sun, could well be a thermophilic habitat for microbes and thus a source of thermophilic microbes growing around 50°C – 60°C. To support this hypothesis, we reviewed the heat sources on earth and associated microbial habitats, as well as the difficult identification of thermophilic microbes. We further present an experim

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Ragini, Arora, and Jha Harit. "Isolation and Characterization of Antioxidant-Producing Thermophilic Bacteria from Tatapani Hot Spring, Chhattisgarh, India." JOURNAL OF CLINICAL AND BIOMEDICAL SCIENCES 15, no. 1 (2025): 38–48. https://doi.org/10.58739/jcbs/v15i1.136.

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Background: Oxidative stress, caused by an imbalance between reactive oxygen species (ROS) and antioxidant defences, is implicated in numerous degenerative diseases. The search for natural antioxidants has led to the exploration of thermophilic bacteria, known for thriving in extreme environments and potentially producing potent antioxidant compounds. Objective: The purpose of this study was to isolate and characterize thermophilic bacteria collected at Tatapani Hot Spring and evaluate their antioxidant potential. Methods: Water samples were collected from Tatapani Hot Spring and subjected to

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Tasturganova, Elmira, Fatima Dikhanbaeva, Alexandr Prosekov, Gulzat Zhunusova, Bagila Dzhetpisbaeva, and Ainur Matibaeva. "Research of Fatty Acid Composition of Samples of Bio-Drink Made of Camel Milk." Current Research in Nutrition and Food Science Journal 6, no. 2 (2018): 491–99. http://dx.doi.org/10.12944/crnfsj.6.2.23.

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Adequate nutrition is the most important determinant of the human health. Taking into account this factor, development of the technology for special purpose dairy products based on camel milk using probiotic starter cultures that will have the ability to destroy toxic metabolites, produce amino acids, volatile fatty acids and synthesize vitamins is the urgent task. In connection with the objective set, we have developed 4 samples of dairy products based on camel milk, and examined fatty acid composition thereof on the basis of the Scientific Research Institute of Biotechnology FSBEI HE Kemerov

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Budagaeva, Valentina Grygoryevna, and Darima Dondokovna Barkhutova. "THERMOPHILIC ORGANOTROPHIC BACTERIA OF THE GENUS MEIOTHERMUS IN ALKALINE HYDROTHERMS OF PRIBAIKALYE (BURYATIA)." Samara Journal of Science 4, no. 2 (2015): 30–32. http://dx.doi.org/10.17816/snv20152108.

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Aerobic, facultative anaerobic hydrolytic bacteria are widespread in the sediments and microbial mats of alkaline hot springs in Baikal region (Buryatia). Typical representatives of hydrolytic bacteria are alkalotermophylic bacilli which are capable of utilizing organic matter in alkaline hot spring waters. Two pure cultures of bacteria growing on acetate, pyruvate and soetone were isolated from the microbial mat of Baikal region thermal springs (Buryatia). These strains were non-sporeforming straight or curved rods that morphologically similar to the representatives of the genus Meiothermus.

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Kha Mong, Tran, Le Thi Thanh Van, Nguyen Vu Phong, and Nguyen Huu Tri. "Isolation and molecular identification of obligate thermophiles from hot springs in Ba Ria – Vung Tau and Khanh Hoa provinces, Vietnam." Vietnam Journal of Biotechnology 20, no. 2 (2022): 379–86. http://dx.doi.org/10.15625/1811-4989/15863.

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Environments with temperatures from 50°C to 80°C are rare in nature and are almost exclusively associated with geothermal regions including hot springs, solar-heated soils and volcanic areas. Thermophilic bacteria already exist and prefer in such habitats. Since innate tolerance to thermal environment and potential chassis for extracellular enzymes such as lipase, protease and amylase, which are utilized widely in the industrial fermentation, thermophilic bacteria have been becoming one of the objects for microbiologists worldwide, recently. This study aimed to isolate and identify thermophili

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Shieh, Wung Yang, and Wen Dar Jean. "Alterococcus agarolyticus, gen.nov., sp.nov., a halophilic thermophilic bacterium capable of agar degradation." Canadian Journal of Microbiology 44, no. 7 (1998): 637–45. http://dx.doi.org/10.1139/w98-051.

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Five strains of facultatively anaerobic moderately thermophilic bacteria were isolated from two hot springs in the intertidal zone of Lutao, Taiwan. They produced extracellular agarase on agar medium, yielding reducing sugars and organic acids as the end products under either aerobic or anaerobic conditions. The growth temperature range was approximately 3858°C with an optimal temperature of about 48°C. The five strains tolerated a relatively narrow pH range from 7.0 to 8.5. They were Gram-negative halophiles growing optimally at 2.02.5% NaCl (ca. 0.340.43 M). They were capable of anaerobic

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Murtiyaningsih, Hidayah, Laras Sekar Arum, and Rendi Anggriawan. "Selection of Polymerase-Producing Thermophilic Bacteria from Ijen Crater, East Java." Indonesian Journal of Biotechnology and Biodiversity 6, no. 1 (2022): 26–32. http://dx.doi.org/10.47007/ijobb.v6i1.123.

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Thermophilic bacteria are a group of bacteria that adapt to environmental conditions with high temperatures, ranging from 45 ° - 90 ° C. as the ring of fire, Indonesia has many volcanic areas as the source of thermophilic bacteria, once is in Ijen Crater, East Java. Thermophilic bacteria have the potential to produce heat-resistant / thermostable enzymes such as polymerase. DNA polymerase is a key enzyme in amplification process of DNA fragment. The objective of this study was to found the novels thermophilic bacteria isolated from Ijen Crater which has potential to be the source of DNA polyme

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Lingga, Rahmad, Budi Afriyansyah, Reti Septiani, and Ina Miranti. "Isolasi dan Karakterisasi Bakteri Asal Sumber Air Panas Non-Vulkanik." BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains 4, no. 2 (2021): 175–84. http://dx.doi.org/10.31539/bioedusains.v4i2.2423.

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This study aims to isolate and characterize bacteria that live in hot springs on Bangka Island. The research methods used include bacterial isolation; morphological characterization, Gram group determination and biochemical tests; identification; and thermophilic bacteria temperature resistance test. Hot water samples were obtained from 2 locations, namely Pemali Village and Permis Village. The hot water temperature range at both locations is between 38ºC to 52ºC. The results showed that 22 pure isolates were consisting of 9 isolates from Permis Village and 13 isolates from Pemali Village. In

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Octarya, Zona, Sumaryati Syukur, and Endang Purwati. "Purifikasi Parsial Enzim Ekstraseluler (Anoxybacillus sp.) yang Diisolasi dari Sumber Air Panas Bukit Kili Solok serta Aplikasinya untuk Menghidrolisis Limbah Berserat." Jurnal Natur Indonesia 15, no. 2 (2015): 106. http://dx.doi.org/10.31258/jnat.15.2.106-114.

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Termostable enzyme from thermophilic bacteria is very potential to improve technical enzyme in industry which used hightemperature. High water temperature exerts selection pressure on microbial species leading to specific flora that survivesand tolerates heat stress. The relative isolation and unique physical properties of Bukit Kili Ketek Hot Springs in Solok,West Sumatera may yield unique thermophiles. The generation of extracellular enzymatic bacterial is highly desirable forproduction of hydrolitic enzymes, which are useful in various industrial application and in animal feeds. This study,

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Lee, Yong-Jik, Dariimaa Ganbat, DoKyung Oh, et al. "Isolation and Characterization of Thermophilic Bacteria from Hot Springs in Republic of Korea." Microorganisms 10, no. 12 (2022): 2375. http://dx.doi.org/10.3390/microorganisms10122375.

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Thermophiles that produce extracellular hydrolases are of great importance due to their applications in various industries. Thermophilic enzymes are of interest for industrial applications due to their compatibility with industrial processes, and the availability of the organisms is essential to develop their full potential. In this study, a culture-dependent approach was used to identify thermophilic bacteria from five hot springs in Republic of Korea. Characterization, taxonomic identification, and extracellular hydrolase (amylase, lipase, and protease) activity of 29 thermophilic bacterial

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Muqarramah, Mufidhatul, Anthoni Agustien, and Feskaharny Alamsjah. "Isolation, Screening and Partial Characterization of Thermophilic Bacteria Producing Protease from Bukik Gadang Hot Springs, Solok Regency." International Journal of Progressive Sciences and Technologies 40, no. 1 (2023): 101. http://dx.doi.org/10.52155/ijpsat.v40.1.4521.

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Research about the isolation, screening and partial characterization of thermophilic bacteria that produce protease from Bukik Gadang hot springs, Solok Regency, was carried out at the Basic Biology Laboratory, UPT Basic and Central Laboratory, Andalas University in January - April 2022. This study aims to obtain isolates of thermophilic bacteria that indicated protease production and to determine the partial characterization of thermophilic bacteria that indicated protease production from Bukik Gadang hot springs, Solok Regency. This study used a survey method and purposive sampling technique

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Łubkowska, Beata, Joanna Jeżewska-Frąckowiak, Ireneusz Sobolewski, and Piotr M. Skowron. "Bacteriophages of Thermophilic ‘Bacillus Group’ Bacteria—A Review." Microorganisms 9, no. 7 (2021): 1522. http://dx.doi.org/10.3390/microorganisms9071522.

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Bacteriophages of thermophiles are of increasing interest owing to their important roles in many biogeochemical, ecological processes and in biotechnology applications, including emerging bionanotechnology. However, due to lack of in-depth investigation, they are underrepresented in the known prokaryotic virosphere. Therefore, there is a considerable potential for the discovery of novel bacteriophage-host systems in various environments: marine and terrestrial hot springs, compost piles, soil, industrial hot waters, among others. This review aims at providing a reference compendium of thermoph

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Zang, Xiangyun, Meiting Liu, Han Wang та ін. "The distribution of active β-glucosidase-producing microbial communities in composting". Canadian Journal of Microbiology 63, № 12 (2017): 998–1008. http://dx.doi.org/10.1139/cjm-2017-0368.

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The composting ecosystem is a suitable source for the discovery of novel microorganisms and secondary metabolites. Cellulose degradation is an important part of the global carbon cycle, and β-glucosidases complete the final step of cellulose hydrolysis by converting cellobiose to glucose. This work analyzes the succession of β-glucosidase-producing microbial communities that persist throughout cattle manure – rice straw composting, and evaluates their metabolic activities and community advantage during the various phases of composting. Fungal and bacterial β-glucosidase genes belonging to glyc

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Brochier-Armanet, Céline, and Patrick Forterre. "Widespread distribution of archaeal reverse gyrase in thermophilic bacteria suggests a complex history of vertical inheritance and lateral gene transfers." Archaea 2, no. 2 (2006): 83–93. http://dx.doi.org/10.1155/2006/582916.

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Reverse gyrase, an enzyme of uncertain funtion, is present in all hyperthermophilic archaea and bacteria. Previous phylogenetic studies have suggested that the gene for reverse gyrase has an archaeal origin and was transferred laterally (LGT) to the ancestors of the two bacterial hyperthermophilic phyla, Thermotogales and Aquificales. Here, we performed an in-depth analysis of the evolutionary history of reverse gyrase in light of genomic progress. We found genes coding for reverse gyrase in the genomes of several thermophilic bacteria that belong to phyla other than Aquificales and Thermotoga

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McMullan, G., J. M. Christie, T. J. Rahman, I. M. Banat, N. G. Ternan, and R. Marchant. "Habitat, applications and genomics of the aerobic, thermophilic genus Geobacillus." Biochemical Society Transactions 32, no. 2 (2004): 214–17. http://dx.doi.org/10.1042/bst0320214.

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Thermophilic bacteria belonging to Bacillus genetic group 5 have been reclassified as being members of Geobacillus gen. nov., with G. stearothermophilus as the type strain. Geobacillus species, literally meaning earth or soil Bacillus, are widely distributed and readily isolated from natural and man-made thermophilic biotopes. Work within our group has however shown that an abundance of genetically distinct Geobacillus isolates can be obtained from temperate Irish soils. As with many thermophiles there is considerable interest in potential industrial application of these bacteria and their gen

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Bai, Jing, Jiang Kang Wen, Song Tao Huang, Biao Wu, and Bo Wei Chen. "The Bioleaching Characteristics of Chalcopyrite with Different Genetic Types." Advanced Materials Research 825 (October 2013): 443–46. http://dx.doi.org/10.4028/www.scientific.net/amr.825.443.

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The copper extraction yield from thermophilic bioleaching of chalcopyrite depends on temperature, pH, and the oxidation-reduction potential (ORP), the activity of the thermophile used, as well as on the different genetic type of the chalcopyrite used. The bioleaching characteristics of chalcopyrite from marine volcanic type and porphyry type, and the influence of genetic type on microbial community were studied. The results indicated that the bioleaching of chalcopyrite is controlled by the ORP rather than by the pH. The thermophiles composition of marine volcanic type bioleaching wasMetallosp

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Suddin, Simanjuntak, Yermia Semuel Mokosuli, Welerubun Marcelina, Naharia Orbanus, and Kapahang Ardi. "Molecular Barcoding Based 16S rRNA Gene of Thermophilic Bacteria from Vulcanic Sites, Linow Lake, Tomohon." Materials Science Forum 967 (August 2019): 83–92. http://dx.doi.org/10.4028/www.scientific.net/msf.967.83.

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Thermophilic bacteria live at temperatures above 450 C. Many investigations focused on their potential as sources of highly active enzymes ‘termostable enzyme’ and other products such as antibiotics and compatible solutes. Lake Linow is an active volcanic lake located in Tomohon City, North Sulawesi, Indonesia. Lake Linow becomes the habitat of thermophilic bacteria. A study has been conducted to obtain isolates of thermophilic bacteria and to identifikasi berdasarkan gen 16 s RNA. Bacterial DNA extraction procedure using the Presto TM Mini gDNA Bacteria Kit Geneaid protocol, with modification

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Lyon, Pierre-François, Trello Beffa, Michel Blanc, Georg Auling, and Michel Aragno. "Isolation and characterization of highly thermophilic xylanolyticThermus thermophilusstrains from hot composts." Canadian Journal of Microbiology 46, no. 11 (2000): 1029–35. http://dx.doi.org/10.1139/w00-075.

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This is the first detailed report of xylanolytic activity in Thermus strains. Two highly thermophilic xylanolytic bacteria, very closely related to non-xylanolytic T. thermophilus strains, have been isolated from the hottest zones of compost piles. Strain X6 was investigated in more detail. The growth rate (optical density monitoring) on xylan was 0.404·h-1at 75°C. Maximal growth temperature was 81°C. Xylanase activity was mainly cell-bound, but was solubilized into the medium by sonication. It was induced by xylan or xylose in the culture medium. The temperature and pH optima of the xylanases

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Lande, Fidhia Rara, Wahyu Widayat, and Yurika Sastyarina. "Isolasi Bakteri Termofilik dari Tanah Hutan Mangrove." Proceeding of Mulawarman Pharmaceuticals Conferences 10 (February 5, 2020): 156–59. http://dx.doi.org/10.25026/mpc.v10i1.383.

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Mangrove forest soil is a habitat and source of diversity of microorganisms, including thermophilic bacteria. The utilization of thermophilic bacteria has been carried out in the pharmaceutical field, especially as a source of raw materials for pharmaceutical products. This study aims to provide an overview of the population of thermophilic bacteria found in mangrove forest soils. Soil samples were obtained by random stratification with a depth of 5-10 cm at 9 points from 3 strata. The isolation media used were Starch Casein Agar (SCA), and the groups of bacteria determined by using the Gram s

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Kurtböke, D. I., N. E. Murphy, and K. Sivasithamparam. "Use of bacteriophage for the selective isolation of thermophilic actinomycetes from composted eucalyptus bark." Canadian Journal of Microbiology 39, no. 1 (1993): 46–51. http://dx.doi.org/10.1139/m93-007.

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A method was developed to reduce the numbers of thermophilic bacteria on isolation plates, which in turn facilitated the detection and isolation of thermophilic actinomycetes. The method involves exposing the test material to bacteriophage suspensions prior to inoculation on isolation plates. This method was applied to composted eucalyptus bark samples, which were then inoculated on R8 and 1/2 TSA + 0.2% casein hydrolysate agar plates. The phage susceptibility of thermophilic bacteria provided a selective means of reducing their numbers on isolation plates and hence increased the numbers of Th

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