Log in

Relevant bibliographies by topics / Thermophilic temperature / Journal articles

To see the other types of publications on this topic, follow the link: Thermophilic temperature.

Journal articles on the topic 'Thermophilic temperature'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Thermophilic temperature.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Hori, Hiroyuki, Takuya Kawamura, Takako Awai, et al. "Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA." Microorganisms 6, no. 4 (2018): 110. http://dx.doi.org/10.3390/microorganisms6040110.

Full text

Abstract:

To date, numerous modified nucleosides in tRNA as well as tRNA modification enzymes have been identified not only in thermophiles but also in mesophiles. Because most modified nucleosides in tRNA from thermophiles are common to those in tRNA from mesophiles, they are considered to work essentially in steps of protein synthesis at high temperatures. At high temperatures, the structure of unmodified tRNA will be disrupted. Therefore, thermophiles must possess strategies to stabilize tRNA structures. To this end, several thermophile-specific modified nucleosides in tRNA have been identified. Othe

APA, Harvard, Vancouver, ISO, and other styles

2

Vavitsas, Konstantinos, Panayiotis D. Glekas, and Dimitris G. Hatzinikolaou. "Synthetic Biology of Thermophiles: Taking Bioengineering to the Extremes?" Applied Microbiology 2, no. 1 (2022): 165–74. http://dx.doi.org/10.3390/applmicrobiol2010011.

Full text

Abstract:

Synthetic biology applications rely on a well-characterized set of microbial strains, with an established toolbox of molecular biology methods for their genetic manipulation. Since there are no thermophiles with such attributes, most biotechnology and synthetic biology studies use organisms that grow in the mesophilic temperature range. As a result, thermophiles, a heterogenous group of microbes that thrive at high (>50 °C) temperatures, are largely overlooked, with respect to their biotechnological potential, even though they share several favorable traits. Thermophilic bacteria tend to gr

APA, Harvard, Vancouver, ISO, and other styles

3

Allgood, Gregory S., and Jerome J. Perry. "Oxygen defense systems in obligately thermophilic bacteria." Canadian Journal of Microbiology 31, no. 11 (1985): 1006–10. http://dx.doi.org/10.1139/m85-190.

Full text

Abstract:

Ten strains of Gram-negative, aerobic, obligately thermophilic bacteria were examined for their response to oxygen toxicity by comparing static with shaken cultures. All of the organisms tested had measurable levels of superoxide dismutase, catalase, and peroxidase. Aeration generally did not result in an increased level of superoxide dismutase in any of the thermophiles. Aeration of organisms obligate for n-alkane substrate caused an increase in cellular peroxidase levels and a corresponding decrease in catalase. The thermophiles that grew on either n-alkanes or complex media did not grow on

APA, Harvard, Vancouver, ISO, and other styles

4

Saner, A. B., and A. K. Mungray. "Distillery Plant Upflow Anaerobic Sludge Blanket Reactor comparision of Energy regime in Mesophilic and Thermophilic conditions." Research Journal of Chemistry and Environment 27, no. 11 (2023): 35–44. http://dx.doi.org/10.25303/2711rjce035044.

Full text

Abstract:

In this study, comparison of energy regime analysis of mesophilic and thermophilic condition upflow anaerobic sludge blanket (UASB) reactor treating distillery effluent is studied. Percentage COD reduction in thermophilic condition was more by 6.96% than mesophilic condition due to which biogas production increased by 0.1 m3/kg COD removed. In thermophilic temperature condition, methenogenesis was more than acidification than in mesophilic temperature condition; effluent VFA concentration in mesophilic condition was more in thermophilic condition. Stability of thermophilic reactor was more tha

APA, Harvard, Vancouver, ISO, and other styles

5

Maheshwari, Ramesh, Girish Bharadwaj, and Mahalingeshwara K. Bhat. "Thermophilic Fungi: Their Physiology and Enzymes." Microbiology and Molecular Biology Reviews 64, no. 3 (2000): 461–88. http://dx.doi.org/10.1128/mmbr.64.3.461-488.2000.

Full text

Abstract:

SUMMARY Thermophilic fungi are a small assemblage in mycota that have a minimum temperature of growth at or above 20°C and a maximum temperature of growth extending up to 60 to 62°C. As the only representatives of eukaryotic organisms that can grow at temperatures above 45°C, the thermophilic fungi are valuable experimental systems for investigations of mechanisms that allow growth at moderately high temperature yet limit their growth beyond 60 to 62°C. Although widespread in terrestrial habitats, they have remained underexplored compared to thermophilic species of eubacteria and archaea. Howe

APA, Harvard, Vancouver, ISO, and other styles

6

Cockell, Charles S., Claire Cousins, Paul T. Wilkinson, Karen Olsson-Francis, and Ben Rozitis. "Are thermophilic microorganisms active in cold environments?" International Journal of Astrobiology 14, no. 3 (2014): 457–63. http://dx.doi.org/10.1017/s1473550414000433.

Full text

Abstract:

AbstractThe mean air temperature of the Icelandic interior is below 10 °C. However, we have previously observed 16S rDNA sequences associated with thermophilic lineages in Icelandic basalts. Measurements of the temperatures of igneous rocks in Iceland showed that solar insolation of these low albedo substrates achieved a peak surface temperature of 44.5 °C. We isolated seven thermophilicGeobacillusspecies from basalt with optimal growth temperatures of ~65 °C. The minimum growth temperature of these organisms was ~36 °C, suggesting that they could be active in the rock environment. Basalt diss

APA, Harvard, Vancouver, ISO, and other styles

7

Pollo, Stephen M. J., Olga Zhaxybayeva, and Camilla L. Nesbø. "Insights into thermoadaptation and the evolution of mesophily from the bacterial phylum Thermotogae." Canadian Journal of Microbiology 61, no. 9 (2015): 655–70. http://dx.doi.org/10.1139/cjm-2015-0073.

Full text

Abstract:

Thermophiles are extremophiles that grow optimally at temperatures >45 °C. To survive and maintain function of their biological molecules, they have a suite of characteristics not found in organisms that grow at moderate temperature (mesophiles). At the cellular level, thermophiles have mechanisms for maintaining their membranes, nucleic acids, and other cellular structures. At the protein level, each of their proteins remains stable and retains activity at temperatures that would denature their mesophilic homologs. Conversely, cellular structures and proteins from thermophiles may not func

APA, Harvard, Vancouver, ISO, and other styles

8

Wilson, Caitlin K., and Gary M. King. "Short-Term Exposure to Thermophilic Temperatures Facilitates CO Uptake by Thermophiles Maintained under Predominantly Mesophilic Conditions." Microorganisms 10, no. 3 (2022): 656. http://dx.doi.org/10.3390/microorganisms10030656.

Full text

Abstract:

Three phylogenetically and phenotypically distinct CO-oxidizing thermophiles (Alicyclobacillus macrosporangiidus CPP55 (Firmicutes), Meiothermus ruber PS4 (Deinococcus-Thermus) and Thermogemmatispora carboxidovorans PM5T (Chloroflexi)) and one CO-oxidizing mesophile (Paraburkholderia paradisi WAT (Betaproteobacteria)) isolated from volcanic soils were used to assess growth responses and CO uptake rates during incubations with constant temperatures (25 °C and 55 °C) and during multi-day incubations with a temperature regime that cycled between 20 °C and 55 °C on a diurnal basis (alternating mes

APA, Harvard, Vancouver, ISO, and other styles

9

Korehi, Hananeh, and Axel Schippers. "Bioleaching of a Marine Hydrothermal Sulfide Ore with Mesophiles, Moderate Thermophiles and Thermophiles." Advanced Materials Research 825 (October 2013): 229–32. http://dx.doi.org/10.4028/www.scientific.net/amr.825.229.

Full text

Abstract:

Marine hydrothermal polymetallic sulfide ores contain high amounts of valuable metals such as Cu, Pb, Zn, Au, Ag, as well as In, Ge, Bi, and Se. Samples from a site in the Indian Ocean were taken during a BGR ship cruise, crushed and sieved for bioleaching experiments to reveal the extraction of the various metals. Chalcopyrite was the main mineral, the total copper content was 38.5 %wt. Comparative bioleaching with mesophilic, moderate thermophilic and thermophilic acidophilic iron- and sulfur-oxidizing bacteria and archaea was investigated. Batch culture experiments were conducted at 2% (w/v

APA, Harvard, Vancouver, ISO, and other styles

10

DiGiacomo, Juliana, Christopher McKay, and Alfonso Davila. "ThermoBase: A database of the phylogeny and physiology of thermophilic and hyperthermophilic organisms." PLOS ONE 17, no. 5 (2022): e0268253. http://dx.doi.org/10.1371/journal.pone.0268253.

Full text

Abstract:

Thermophiles and hyperthermophiles are those organisms which grow at high temperature (> 40°C). The unusual properties of these organisms have received interest in multiple fields of biological research, and have found applications in biotechnology, especially in industrial processes. However, there are few listings of thermophilic and hyperthermophilic organisms and their relevant environmental and physiological data. Such repositories can be used to standardize definitions of thermophile and hyperthermophile limits and tolerances and would mitigate the need for extracting organism data fr

APA, Harvard, Vancouver, ISO, and other styles

11

Adhikari, Hriush, Sangam Ghimire, Binod Khatri, and Yuvraj K.C. "Enzymatic Screening and Molecular Characterization of Thermophilic Bacterial Strains Isolated from Hotspring of Tatopani, Bhurung, Nepal." International Journal of Applied Sciences and Biotechnology 3, no. 3 (2015): 392–97. http://dx.doi.org/10.3126/ijasbt.v3i3.12724.

Full text

Abstract:

Background and Aim: In Nepal not much of study of Thermophilic area and Thermophiles have been done. Thermophilic bacteria are less studied but are important group of microorganisms due to their ability to produce industrially important enzymes. Methods: In this study, thermophilic bacteria were isolated from hot spring of Bhurung, Nepal. Wide range of bacteria that could grow at high temperatures and tolerate extreme temperature were characterized by morphology, biochemistry and sequencing of its 16S rRNA gene sequence. The isolates were screened for production of extracellular enzymes like p

APA, Harvard, Vancouver, ISO, and other styles

12

Kovács, R., P. Miháltz, and Zs Csikor. "Kinetics of autothermal thermophilic aerobic digestion – application and extension of Activated Sludge Model No 1 at thermophilic temperatures." Water Science and Technology 56, no. 9 (2007): 137–45. http://dx.doi.org/10.2166/wst.2007.706.

Full text

Abstract:

The application of an ASM1-based mathematical model for the modeling of autothermal thermophilic aerobic digestion is demonstrated. Based on former experimental results the original ASM1 was extended by the activation of facultative thermophiles from the feed sludge and a new component, the thermophilic biomass was introduced. The resulting model was calibrated in the temperature range of 20–60 °C. The temperature dependence of the growth and decay rates in the model is given in terms of the slightly modified Arrhenius and Topiwala-Sinclair equations. The capabilities of the calibrated model i

APA, Harvard, Vancouver, ISO, and other styles

13

Wouters, Jeroen A., Frank M. Rombouts, Willem M. de Vos, Oscar P. Kuipers, and Tjakko Abee. "Cold Shock Proteins and Low-Temperature Response ofStreptococcus thermophilus CNRZ302." Applied and Environmental Microbiology 65, no. 10 (1999): 4436–42. http://dx.doi.org/10.1128/aem.65.10.4436-4442.1999.

Full text

Abstract:

ABSTRACT Low-temperature adaptation and cryoprotection were studied in the thermophilic lactic acid bacterium Streptococcus thermophilus CNRZ302. S. thermophilus actively adapts to freezing during a pretreatment at 20°C, resulting in an approximately 1,000-fold increased survival after four freeze-thaw cycles compared to mid-exponential-phase cells grown at an optimal temperature of 42°C. No adaptation is observed when cells are exposed to a temperature (10°C) below the minimal growth temperature of the strain (just below 15°C). By two-dimensional gel electrophoresis several 7-kDa cold-induced

APA, Harvard, Vancouver, ISO, and other styles

14

Kumar, Vijay, Nikhil Sharma та Tek Chand Bhalla. "In Silico Analysis of β-Galactosidases Primary and Secondary Structure in relation to Temperature Adaptation". Journal of Amino Acids 2014 (24 березня 2014): 1–9. http://dx.doi.org/10.1155/2014/475839.

Full text

Abstract:

β-D-Galactosidases (EC 3.2.1.23) hydrolyze the terminal nonreducing β-D-galactose residues in β-D-galactosides and are ubiquitously present in all life forms including extremophiles. Eighteen microbial β-galactosidase protein sequences, six each from psychrophilic, mesophilic, and thermophilic microbes, were analyzed. Primary structure reveals alanine, glycine, serine, and arginine to be higher in psychrophilic β-galactosidases whereas valine, glutamine, glutamic acid, phenylalanine, threonine, and tyrosine are found to be statistically preferred by thermophilic β-galactosidases. Cold active β

APA, Harvard, Vancouver, ISO, and other styles

15

Hamzah, Muhammad Arif Fikri, Jamaliah Md Jahim, Peer Mohamed Abdul, and Ahmad Jaril Asis. "Investigation of Temperature Effect on Start-Up Operation from Anaerobic Digestion of Acidified Palm Oil Mill Effluent." Energies 12, no. 13 (2019): 2473. http://dx.doi.org/10.3390/en12132473.

Full text

Abstract:

Malaysia is one of the largest palm oil producers worldwide and its most abundant waste, palm oil mill effluent (POME), can be used as a feedstock to produce methane. Anaerobic digestion is ideal for treating POME in methane production due to its tolerance to high-strength chemical oxygen demand (COD). In this work, we compared the culture conditions during the start-up of anaerobic digestion of acidified POME between thermophilic (55 °C) and mesophilic (37 °C) temperatures. The pH of the digester was maintained throughout the experiment at 7.30 ± 0.2 in a working volume of 1000 mL. This study

APA, Harvard, Vancouver, ISO, and other styles

16

Bai, Jing, Jiang Kang Wen, Song Tao Huang, Biao Wu, and Bo Wei Chen. "The Bioleaching Characteristics of Chalcopyrite with Different Genetic Types." Advanced Materials Research 825 (October 2013): 443–46. http://dx.doi.org/10.4028/www.scientific.net/amr.825.443.

Full text

Abstract:

The copper extraction yield from thermophilic bioleaching of chalcopyrite depends on temperature, pH, and the oxidation-reduction potential (ORP), the activity of the thermophile used, as well as on the different genetic type of the chalcopyrite used. The bioleaching characteristics of chalcopyrite from marine volcanic type and porphyry type, and the influence of genetic type on microbial community were studied. The results indicated that the bioleaching of chalcopyrite is controlled by the ORP rather than by the pH. The thermophiles composition of marine volcanic type bioleaching wasMetallosp

APA, Harvard, Vancouver, ISO, and other styles

17

Thomas, Torsten, Naresh Kumar, and Ricardo Cavicchioli. "Effects of Ribosomes and Intracellular Solutes on Activities and Stabilities of Elongation Factor 2 Proteins from Psychrotolerant and Thermophilic Methanogens." Journal of Bacteriology 183, no. 6 (2001): 1974–82. http://dx.doi.org/10.1128/jb.183.6.1974-1982.2001.

Full text

Abstract:

ABSTRACT Low-temperature-adapted archaea are abundant in the environment, yet little is known about the thermal adaptation of their proteins. We have previously compared elongation factor 2 (EF-2) proteins from Antarctic (Methanococcoides burtonii) and thermophilic (Methanosarcina thermophila) methanogens and found that theM. burtonii EF-2 had greater intrinsic activity at low temperatures and lower thermal stability at high temperatures (T. Thomas and R. Cavicchioli, J. Bacteriol. 182:1328–1332, 2000). While the gross thermal properties correlated with growth temperature, the activity and sta

APA, Harvard, Vancouver, ISO, and other styles

18

Takai, Ken, Takuro Nunoura, Yoshihiko Sako, and Aritsune Uchida. "Acquired Thermotolerance and Temperature-Induced Protein Accumulation in the Extremely Thermophilic BacteriumRhodothermus obamensis." Journal of Bacteriology 180, no. 10 (1998): 2770–74. http://dx.doi.org/10.1128/jb.180.10.2770-2774.1998.

Full text

Abstract:

ABSTRACT Temperature-induced changes in thermotolerance and protein composition were examined in heat-shocked cells and high-temperature-grown cells of the extremely thermophilic bacteriumRhodothermus obamensis. The survival at temperatures superoptimal for growth (90 and 95°C) was enhanced in both heat-shocked cells and high-temperature-grown cells relative to that of cells grown at optimal temperatures. In a comparison of protein composition using two-dimensional gel electrophoresis, putative heat shock proteins (HSPs) and high-temperature growth-specific proteins (HGPs) were detected. N-ter

APA, Harvard, Vancouver, ISO, and other styles

19

Lyon, Pierre-François, Trello Beffa, Michel Blanc, Georg Auling, and Michel Aragno. "Isolation and characterization of highly thermophilic xylanolyticThermus thermophilusstrains from hot composts." Canadian Journal of Microbiology 46, no. 11 (2000): 1029–35. http://dx.doi.org/10.1139/w00-075.

Full text

Abstract:

This is the first detailed report of xylanolytic activity in Thermus strains. Two highly thermophilic xylanolytic bacteria, very closely related to non-xylanolytic T. thermophilus strains, have been isolated from the hottest zones of compost piles. Strain X6 was investigated in more detail. The growth rate (optical density monitoring) on xylan was 0.404·h-1at 75°C. Maximal growth temperature was 81°C. Xylanase activity was mainly cell-bound, but was solubilized into the medium by sonication. It was induced by xylan or xylose in the culture medium. The temperature and pH optima of the xylanases

APA, Harvard, Vancouver, ISO, and other styles

20

Nguyen, Tri H. "Isolation and optimization of the growth conditions of thermophilic microorganism from hot springs." Journal of Agriculture and Development 17, no. 03 (2018): 55–60. http://dx.doi.org/10.52997/jad.8.03.2018.

Full text

Abstract:

The aim of this study was to isolate and optimize the growth conditions of thermophilic microorganism from hot springs. The isolation was conducted by using the mineral salt basal medium supplemented with 0.6% yeast extract at 50oC. Totally, 33 isolates of thermophilic microorganism were isolated from hot springs at Truong Xuan (Khanh Hoa province) and Binh Chau (Ba Ria - Vung Tau province). The effects of temperature (45 - 80oC), pH (pH 6 - 9) and carbon sources (malate, pyruvate, acetate, glucose, fructose, or carbon dioxide) on the growth of isolates were examined. In addition, the isolate

APA, Harvard, Vancouver, ISO, and other styles

21

Džupponová, Veronika, Nataša Tomášková, Andrea Antošová, Erik Sedlák, and Gabriel Žoldák. "Salt-Specific Suppression of the Cold Denaturation of Thermophilic Multidomain Initiation Factor 2." International Journal of Molecular Sciences 24, no. 7 (2023): 6787. http://dx.doi.org/10.3390/ijms24076787.

Full text

Abstract:

Thermophilic proteins and enzymes are attractive for use in industrial applications due to their resistance against heat and denaturants. Here, we report on a thermophilic protein that is stable at high temperatures (Ttrs, hot 67 °C) but undergoes significant unfolding at room temperature due to cold denaturation. Little is known about the cold denaturation of thermophilic proteins, although it can significantly limit their applications. We investigated the cold denaturation of thermophilic multidomain protein translation initiation factor 2 (IF2) from Thermus thermophilus. IF2 is a GTPase tha

APA, Harvard, Vancouver, ISO, and other styles

22

Budagaeva, Valentina Grygoryevna, and Darima Dondokovna Barkhutova. "THERMOPHILIC ORGANOTROPHIC BACTERIA OF THE GENUS MEIOTHERMUS IN ALKALINE HYDROTHERMS OF PRIBAIKALYE (BURYATIA)." Samara Journal of Science 4, no. 2 (2015): 30–32. http://dx.doi.org/10.17816/snv20152108.

Full text

Abstract:

Aerobic, facultative anaerobic hydrolytic bacteria are widespread in the sediments and microbial mats of alkaline hot springs in Baikal region (Buryatia). Typical representatives of hydrolytic bacteria are alkalotermophylic bacilli which are capable of utilizing organic matter in alkaline hot spring waters. Two pure cultures of bacteria growing on acetate, pyruvate and soetone were isolated from the microbial mat of Baikal region thermal springs (Buryatia). These strains were non-sporeforming straight or curved rods that morphologically similar to the representatives of the genus Meiothermus.

APA, Harvard, Vancouver, ISO, and other styles

23

Kim and Lee. "Effects of a Groundwater Heat Pump on Thermophilic Bacteria Activity." Water 11, no. 10 (2019): 2084. http://dx.doi.org/10.3390/w11102084.

Full text

Abstract:

Groundwater samples were collected from the tubular wells of a groundwater heat pump (GWHP), and the psychrophilic, mesophilic, and thermophilic bacteria inhabiting the collected groundwater were cultured and isolated. Using the isolated bacteria, we analyzed temperature-dependent changes in autochthonous bacteria based on the operation of the GWHP. Microbial culture identified eight species of bacteria: five species of thermophilic bacteria (Anoxybacillus tepidamans, Bacillus oceanisediminis, Deinococcus geothermalis, Effusibacillus pohliae, and Vulcaniibacterium thermophilum), one species of

APA, Harvard, Vancouver, ISO, and other styles

24

Nesbø, Camilla L., Marlena Dlutek, Olga Zhaxybayeva, and W. Ford Doolittle. "Evidence for Existence of “Mesotogas,” Members of the Order Thermotogales Adapted to Low-Temperature Environments." Applied and Environmental Microbiology 72, no. 7 (2006): 5061–68. http://dx.doi.org/10.1128/aem.00342-06.

Full text

Abstract:

ABSTRACT All cultivated isolates of the bacterial order Thermotogales are either thermophiles or hyperthermophiles, but Thermotogales 16S rRNA gene sequences have been detected in many mesophilic anaerobic and microaerophilic environments, particularly within communities involved in the remediation of pollutants. Here we provide metagenomic evidence for the existence of Thermotogales lineages, which we informally call “mesotoga,” that are adapted to growth at lower temperatures. Two fosmid clones containing mesotoga DNA, originating from a low-temperature enrichment culture that degrades a pol

APA, Harvard, Vancouver, ISO, and other styles

25

Ahring, B. K. "Status on science and application of thermophilic anaerobic digestion." Water Science and Technology 30, no. 12 (1994): 241–49. http://dx.doi.org/10.2166/wst.1994.0619.

Full text

Abstract:

Thermophilic anaerobic processes are often regarded as less stable than mesophilic processes. In the paper this postulate is examined and disproved based on real operational data from of full-scale mesophilic and thermophilic biogas plants. The start-up procedure for the thermophilic plants was, however, found to be crucial for determining the period needed before full capacity of the thermophilic plant could be achieved. The importance of balanced loading, determined by the concentration of active biomass, is discussed. Finally, data will be shown on the optimal and maximal temperature for th

APA, Harvard, Vancouver, ISO, and other styles

26

Hoffmann, Anne, Christian Lorenz, Jörg Fallmann, et al. "Temperature-Dependent tRNA Modifications in Bacillales." International Journal of Molecular Sciences 25, no. 16 (2024): 8823. http://dx.doi.org/10.3390/ijms25168823.

Full text

Abstract:

Transfer RNA (tRNA) modifications are essential for the temperature adaptation of thermophilic and psychrophilic organisms as they control the rigidity and flexibility of transcripts. To further understand how specific tRNA modifications are adjusted to maintain functionality in response to temperature fluctuations, we investigated whether tRNA modifications represent an adaptation of bacteria to different growth temperatures (minimal, optimal, and maximal), focusing on closely related psychrophilic (P. halocryophilus and E. sibiricum), mesophilic (B. subtilis), and thermophilic (G. stearother

APA, Harvard, Vancouver, ISO, and other styles

27

THOMAS, M. Theresa, and K. Robert SCOPES. "The effects of temperature on the kinetics and stability of mesophilic and thermophilic 3-phosphoglycerate kinases." Biochemical Journal 330, no. 3 (1998): 1087–95. http://dx.doi.org/10.1042/bj3301087.

Full text

Abstract:

The effects of temperature on the kinetic parameters kcat and Km, for three isolates of the highly conserved monomeric enzyme 3-phosphoglycerate kinase (PGK), were investigated in detail using a rapid automated kinetics apparatus. PGK was purified from the thermophilic bacterium Thermoanaerobacter sp. Rt8.G4 (optimum growth temperature 68 °C), the mesophile Zymomonas mobilis (optimum growth temperature 32 °C) and a second, unidentified, soil mesophile designated unid A (optimum growth temperature 27 °C). The kinetic behaviour with temperature of each PGK preparation was distinct, despite the c

APA, Harvard, Vancouver, ISO, and other styles

28

Pennacchio, Angela, Biagio Pucci, Francesco Secundo, Francesco La Cara, Mosè Rossi, and Carlo A. Raia. "Purification and Characterization of a Novel Recombinant Highly Enantioselective Short-Chain NAD(H)-Dependent Alcohol Dehydrogenase from Thermus thermophilus." Applied and Environmental Microbiology 74, no. 13 (2008): 3949–58. http://dx.doi.org/10.1128/aem.00217-08.

Full text

Abstract:

ABSTRACT The gene encoding a novel alcohol dehydrogenase (ADH) that belongs to the short-chain dehydrogenase/reductase (SDR) superfamily was identified in the extremely thermophilic, halotolerant gram-negative eubacterium Thermus thermophilus HB27. The T. thermophilus ADH gene (adh Tt) was heterologously overexpressed in Escherichia coli, and the protein (ADHTt) was purified to homogeneity and characterized. ADHTt is a tetrameric enzyme consisting of identical 26,961-Da subunits composed of 256 amino acids. The enzyme has remarkable thermophilicity and thermal stability, displaying activity at

APA, Harvard, Vancouver, ISO, and other styles

29

Ranneklev, Sissel Brit, and Erland Bååth. "Temperature-Driven Adaptation of the Bacterial Community in Peat Measured by Using Thymidine and Leucine Incorporation." Applied and Environmental Microbiology 67, no. 3 (2001): 1116–22. http://dx.doi.org/10.1128/aem.67.3.1116-1122.2001.

Full text

Abstract:

ABSTRACT The temperature-driven adaptation of the bacterial community in peat was studied, by altering temperature to simulate self-heating and a subsequent return to mesophilic conditions. The technique used consisted of extracting the bacterial community from peat using homogenization-centrifugation and measuring the rates of thymidine (TdR) or leucine (Leu) incorporation by the extracted bacterial community at different temperatures. Increasing the peat incubation temperature from 25°C to 35, 45, or 55°C resulted in a selection of bacterial communities whose optimum temperatures for activit

APA, Harvard, Vancouver, ISO, and other styles

30

Blake, Lynsay I., Angela Sherry, Obioma K. Mejeha, et al. "An Unexpectedly Broad Thermal and Salinity-Tolerant Estuarine Methanogen Community." Microorganisms 8, no. 10 (2020): 1467. http://dx.doi.org/10.3390/microorganisms8101467.

Full text

Abstract:

Moderately thermophilic (Tmax, ~55 °C) methanogens are identified after extended enrichments from temperate, tropical and low-temperature environments. However, thermophilic methanogens with higher growth temperatures (Topt ≥ 60 °C) are only reported from high-temperature environments. A microcosm-based approach was used to measure the rate of methane production and methanogen community structure over a range of temperatures and salinities in sediment from a temperate estuary. We report short-term incubations (<48 h) revealing methanogens with optimal activity reaching 70 °C in a temperate

APA, Harvard, Vancouver, ISO, and other styles

31

Yang, Shan Rang, Yong Zhen Li, Yu Ming Ding, et al. "Effects of Mesophilic and Thermophilic Temperature on Hydrolysis and Acidification of Organic Wastes Two-Phase Anaerobic Digestion." Applied Mechanics and Materials 448-453 (October 2013): 1599–604. http://dx.doi.org/10.4028/www.scientific.net/amm.448-453.1599.

Full text

Abstract:

This paper investigated the effects of mesophilic temperature (35°C) and thermophilic temperature (55°C) on the hydrolysis and acidification phase in the process of organic wastes anaerobic digestion. Here, the organic wastes refer to the mixture of corn straw and pig manure in this paper. The results show that pH value is negatively correlated with volatile fatty acids (VFAs) concentration, throughout the process both the VFAs concentration and its maximum of mesophilic temperature are bigger than that of thermophilic temperature, but it takes longer time to reach the maximum; the total solid

APA, Harvard, Vancouver, ISO, and other styles

32

Sang, Peng, Shu-Qun Liu, and Li-Quan Yang. "New Insight into Mechanisms of Protein Adaptation to High Temperatures: A Comparative Molecular Dynamics Simulation Study of Thermophilic and Mesophilic Subtilisin-Like Serine Proteases." International Journal of Molecular Sciences 21, no. 9 (2020): 3128. http://dx.doi.org/10.3390/ijms21093128.

Full text

Abstract:

In high-temperature environments, thermophilic proteins must possess enhanced thermal stability in order to maintain their normal biological functions. However, the physicochemical basis of the structural stability of thermophilic proteins at high temperatures remains elusive. In this study, we performed comparative molecular dynamics simulations on thermophilic serine protease (THM) and its homologous mesophilic counterpart (PRK). The comparative analyses of dynamic structural and geometrical properties suggested that THM adopted a more compact conformation and exhibited more intramolecular i

APA, Harvard, Vancouver, ISO, and other styles

33

P. Tripathy, Prangya, Ashok Kumar Mohanty, and P. K. Aggarwal. "Proteomic Analysis of Thermophilic Starter Streptococcus thermophilus NCDC74 on Low Temperature." International Journal of Current Microbiology and Applied Sciences 14, no. 2 (2025): 103–15. https://doi.org/10.20546/ijcmas.2025.1402.009.

Full text

Abstract:

Low temperature adaptation of a thermophilic starter Streptococcus thermophilus NCDC74 (St74) (routinely used for production of ‘Dahi’ (yoghurt) in Indian Dairy Industries) was analysed by physiology and proteomic approach. Cold shock at 20?C for 2, 4 and 6h to St74 demonstrated a factorial increase in freeze-thaw survival of 80%, 800% and 250% respectively, compared to the control (42?C). Proteomic analysis of cold-induced cells at 20?C for 6h revealed 23 spots, which were differentially expressed. Of the differentially expressed proteins, 14 and 9 were of low (< 10 kDa) and high molecular

APA, Harvard, Vancouver, ISO, and other styles

34

Wang, Chuan, Yuze Yang, Shaoqing Tang, et al. "Comparison of Structural Features of CRISPR-Cas Systems in Thermophilic Bacteria." Microorganisms 11, no. 9 (2023): 2275. http://dx.doi.org/10.3390/microorganisms11092275.

Full text

Abstract:

The clustered regularly interspaced short palindromic repeat (CRISPR) is an adaptive immune system that defends most archaea and many bacteria from foreign DNA, such as phages, viruses, and plasmids. The link between the CRISPR-Cas system and the optimum growth temperature of thermophilic bacteria remains unclear. To investigate the relationship between the structural characteristics, diversity, and distribution properties of the CRISPR-Cas system and the optimum growth temperature in thermophilic bacteria, genomes of 61 species of thermophilic bacteria with complete genome sequences were down

APA, Harvard, Vancouver, ISO, and other styles

35

GIRAFFA, GIORGIO, DOMENICO CARMINATI, and GIOVANNA TORRI TARELLI. "Inhibition of Listeria innocua in Milk by Bacteriocin-Producing Enterococcus faecium 7C5." Journal of Food Protection 58, no. 6 (1995): 621–23. http://dx.doi.org/10.4315/0362-028x-58.6.621.

Full text

Abstract:

Enterococcus faecium 7C5 produces a bacteriocin active against Listeria monocytogenes and Listeria innocua. In co-cultures of the strain 7C5 with a thermophilic starter, which was composed of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus, the acidifying activity of the latter was not affected. In contrast, the rate of bacteriocin production was lower when compared with the pure culture of strain 7C5. In co-cultures of L. innocua CNRZ LIN 11 with the thermophilic starter or with strain 7C5, a bacteriostatic effect on Listeria growth was observed. In the co-culture o

APA, Harvard, Vancouver, ISO, and other styles

36

Ingersoll, John G. "Thermophilic Fungi as the Microbial Agents of Choice for the Industrial Co-Fermentation of Wood Wastes and Nitrogen-Rich Organic Wastes to Bio-Methane." Microorganisms 11, no. 10 (2023): 2600. http://dx.doi.org/10.3390/microorganisms11102600.

Full text

Abstract:

The novel industrial approach of co-fermenting wood wastes with agricultural wastes that are rich in nitrogen such as animal manures to produce bio-methane (renewable natural gas) fuel via thermophilic anaerobic digestion mimics an analogous process occurring in lower termites, but it relies instead on thermophilic fungi along with other thermophilic microorganisms comprising suitable bacteria and archaea. Wood microbial hydrolysis under thermophilic temperatures (range of 55 °C to 70 °C) and aerobic or micro-aerobic conditions constitutes the first step of the two-step (hydrolysis and ferment

APA, Harvard, Vancouver, ISO, and other styles

37

Sharma, Maurya, Naayaa Mehta, Renuka Suravajhala, Cynthia Meza, Shrabana Sarkar, and Aparna Banerjee. "Temperature-Dependent Structure–Function Properties of Bacterial Xylose Isomerase Enzyme for Food Applications: An In Silico Study." Clean Technologies 4, no. 4 (2022): 1317–29. http://dx.doi.org/10.3390/cleantechnol4040081.

Full text

Abstract:

Xylose Isomerase (XI) is an intramolecular oxidoreductase enzyme and catalyzes the reversible conversion of ketoses and aldoses in addition to the bioconversion of ethanol from xylose in the production of bioethanol from hemicellulose. It has a broad range of industrial applications in the food and pharmaceutical sectors, particularly in the production of the sweetener high fructose corn syrup (HFCS). It is one of the most widely used industrial enzymes after protease. Taking this into consideration, four bacterial XI sources were selected based on growth temperature, i.e., psychrophile, mesop

APA, Harvard, Vancouver, ISO, and other styles

38

Hu, Weijie, Youfei Zhou, Hong Zhu, and Tianfeng Wang. "Effects of One-Step Abrupt Temperature Change on Anaerobic Co-Digestion of Kitchen Waste with Dewatered Sludge." Fermentation 10, no. 1 (2023): 5. http://dx.doi.org/10.3390/fermentation10010005.

Full text

Abstract:

The operating temperature of anaerobic digesters should be adjusted to adapt to seasonal variations in environmental temperature and the composition of organic solid waste. This study investigated the effects of one-step abrupt temperature changes (from mesophilic to thermophilic temperature, M–T, and from thermophilic to mesophilic temperature, T–M) and the inoculation ratio on methane yield and microbial diversity during the anaerobic co-digestion of kitchen waste with dewatered sludge. The results showed that the cumulative methane yield (CMY) level resulting from thermophilic control and t

APA, Harvard, Vancouver, ISO, and other styles

39

Watts, S., G. Hamilton, and J. Keller. "Two-stage thermophilic-mesophilic anaerobic digestion of waste activated sludge from a biological nutrient removal plant." Water Science and Technology 53, no. 8 (2006): 149–57. http://dx.doi.org/10.2166/wst.2006.245.

Full text

Abstract:

A two-stage thermophilic-mesophilic anaerobic digestion pilot-plant was operated solely on waste activated sludge (WAS) from a biological nutrient removal (BNR) plant. The first-stage thermophilic reactor (HRT 2 days) was operated at 47, 54 and 60 °C. The second-stage mesophilic digester (HRT 15 days) was held at a constant temperature of 36–37 °C. For comparison with a single-stage mesophilic process, the mesophilic digester was also operated separately with an HRT of 17 days and temperature of 36–37 °C. The results showed a truly thermophilic stage (60 °C) was essential to achieve good WAS d

APA, Harvard, Vancouver, ISO, and other styles

40

Haider, Syed Zeeshan. "Investigating the effect of temperature gradient on biogas production from pretreated maize straw and rice husk using multistage anaerobic bioreactor." Pakistan Journal of Agricultural Sciences 58, no. 04 (2021): 1339–48. http://dx.doi.org/10.21162/pakjas/21.1521.

Full text

Abstract:

The share of biogas in renewable energy sources is increasing as variety of feedstocks are now used for biogas production among which lignocellulosic biomass is emerging feedstock that can be used after proper pretreatment under best suited temperature. Although lot of pretreatments and temperature combinations have been tested but still there is a gap that can be filled by the current study focused on the effect of the temperature gradient (mesophilic and thermophilic) on biogas production potential of maize straw and rice husk using a modified Gompertz equation. Pretreatment was done by usin

APA, Harvard, Vancouver, ISO, and other styles

41

Alvarez-Ponce, David, Mario Ruiz-González, Francisco Vera-Sirera, Felix Feyertag, Miguel Perez-Amador, and Mario Fares. "Arabidopsis Heat Stress-Induced Proteins Are Enriched in Electrostatically Charged Amino Acids and Intrinsically Disordered Regions." International Journal of Molecular Sciences 19, no. 8 (2018): 2276. http://dx.doi.org/10.3390/ijms19082276.

Full text

Abstract:

Comparison of the proteins of thermophilic, mesophilic, and psychrophilic prokaryotes has revealed several features characteristic to proteins adapted to high temperatures, which increase their thermostability. These characteristics include a profusion of disulfide bonds, salt bridges, hydrogen bonds, and hydrophobic interactions, and a depletion in intrinsically disordered regions. It is unclear, however, whether such differences can also be observed in eukaryotic proteins or when comparing proteins that are adapted to temperatures that are more subtly different. When an organism is exposed t

APA, Harvard, Vancouver, ISO, and other styles

42

Nozhevnikova, Alla N., C. Holliger, A. Ammann, and A. J. B. Zehnder. "Methanogenesis in sediments from deep lakes at different temperatures (2–70°C)." Water Science and Technology 36, no. 6-7 (1997): 57–64. http://dx.doi.org/10.2166/wst.1997.0575.

Full text

Abstract:

Methanogenic degradation of organic matter occurs in a wide temperature range from psychrophilic to extreme thermophilic conditions. Mesophilic and thermophilic methanogenesis is relatively well investigated, but little is known about low temperature methanogenesis and psychrophilic methanogenic communities. The aim of the present work was to study methanogenesis in a wide range of temperatures with samples from sediments of deep lakes. These sediments may be considered deposits of different types of microorganisms, which are constantly exposed to low temperatures. The main question was how ps

APA, Harvard, Vancouver, ISO, and other styles

43

Nozhevnikova, A. N., O. R. Kotsyurbenko, and S. N. Parshina. "Anaerobic manure treatment under extreme temperature conditions." Water Science and Technology 40, no. 1 (1999): 215–21. http://dx.doi.org/10.2166/wst.1999.0046.

Full text

Abstract:

Usually anaerobic treatment of manure is performed as one stage fermentation under mesophilic (30-35°C) or moderate thermophilic (50-55°C) conditions, with the aim to stabilize organic material for the use of digested manure as a fertilizer. The present study was conducted to develop a new approach for manure treatment, which concerned the sanitation of manure and saving energy for its treatment. Laboratory scale experiments were performed on the digestion of cattle and pig manure under psychrophilic conditions (5-20°C) and extreme thermophilic (55-82°C) conditions. Specifically, we were inter

APA, Harvard, Vancouver, ISO, and other styles

44

Mawati, Sefi Desfeni, Esti Harpen, and Hilma Putri Fidyandini. "SKRINING BAKTERI TERMOFILIK POTENSIAL AMILOLITIK DARI SUMBER AIR PANAS WAY BELERANG KALIANDA LAMPUNG SELATAN." Journal of Aquatropica Asia 6, no. 1 (2021): 1–7. http://dx.doi.org/10.33019/aquatropica.v6i1.2458.

Full text

Abstract:

Thermophilic bacteria that produced amylase and protease have been isolated from Way Belerang hot spring, Kalianda, South Lampung. This research aims to screen and identify thermophilic bacteria that have the potential to produce thermostable amylase and protease enzymes.The research procedures included sampling, isolation of enzyme-producing thermophilic bacteria, a series of phenotypic and biochemical tests, and molecular identification by 16s rRNA. This study used 2 treatments, namely incubation temperature 37 and 50 ºC with 3 repetitions. The results showed that the optimum temperature for

APA, Harvard, Vancouver, ISO, and other styles

45

Shieh, Wung Yang, and Wen Dar Jean. "Alterococcus agarolyticus, gen.nov., sp.nov., a halophilic thermophilic bacterium capable of agar degradation." Canadian Journal of Microbiology 44, no. 7 (1998): 637–45. http://dx.doi.org/10.1139/w98-051.

Full text

Abstract:

Five strains of facultatively anaerobic moderately thermophilic bacteria were isolated from two hot springs in the intertidal zone of Lutao, Taiwan. They produced extracellular agarase on agar medium, yielding reducing sugars and organic acids as the end products under either aerobic or anaerobic conditions. The growth temperature range was approximately 3858°C with an optimal temperature of about 48°C. The five strains tolerated a relatively narrow pH range from 7.0 to 8.5. They were Gram-negative halophiles growing optimally at 2.02.5% NaCl (ca. 0.340.43 M). They were capable of anaerobic

APA, Harvard, Vancouver, ISO, and other styles

46

Singh, S., and D. K. Sandhu. "Thermophilous fungi in Port Blair soils." Canadian Journal of Botany 64, no. 5 (1986): 1018–26. http://dx.doi.org/10.1139/b86-139.

Full text

Abstract:

Soils were collected from eight different sites of saline marshy soils of Port Blair situated in the Andaman and Nicobar islands, India. A total of 93 333 colony-forming units representing 46 species of thermophilous fungi were isolated by the soil dilution method. The fungi were tabulated in order of ecological importance based on their frequency, relative density, and presence in the selected sites of this study. This is apparently the first study of these fungi from the Port Blair soils. Of the 46 species, Mucor miehei, Rhizopus oligosporus, Thermoascus crustaceous, T. thermophilus, and Tri

APA, Harvard, Vancouver, ISO, and other styles

47

Fernández-Rodríguez, J., M. Pérez, and L. I. Romero. "Bio-methanization of organic fraction from municipal solid waste: temperature effects." Polish Journal of Chemical Technology 15, no. 2 (2013): 99–106. http://dx.doi.org/10.2478/pjct-2013-0030.

Full text

Abstract:

The main objective of this study was to analyse the development of dry anaerobic digestion process of OFMSW in batch reactors under two temperature ranges, thermophilic (55°C) and mesophilic (35°C). The experimental results lead to the conclusion that the thermophilic range has a greater rate of hydrolysis and is therefore more effective to degrade wastes, shortening the overall operating time. For example, the hydrolytic step in the thermophilic (T) process lasted an average of 8 days versus 14 days in the mesophilic (M) range. The methanogenic phase lasted for 18 and 29 days in the T and M p

APA, Harvard, Vancouver, ISO, and other styles

48

Mohammed, Mohanad Jasim. "INFLUENCE OF TEMPERATURE FLUCTUATION ON THERMOPHILIC ANAEROBIC DIGESTION OF MUNICIPAL ORGANIC WASTE." Journal of Engineering 14, no. 03 (2008): 2901–11. http://dx.doi.org/10.31026/j.eng.2008.03.25.

Full text

Abstract:

A laboratory-scale experiment was carried out to estimate the influence of temperature fluctuation on thermophilic anaerobic digestion of municipal organic waste (MOW). Heating failure was simulated by decreasing temperature gradually from 55 °C to 20 °C gradually 2 h time need for temperature decrease and recovery. Under conditions of 8.0 g/ (L·d) and 15 d respectively for MOW load and retention time. Following results were found: (1) biogas production almost stopped and VFA (Volatile Fatty Acid) accumulated rapidly when accompanied by pH decrease. (2) With low temperature (20°C) duration of

APA, Harvard, Vancouver, ISO, and other styles

49

Huang, Jing Shui, Yue Wen, A. Sheng Cao, Hai Song Li, and Qi Zhou. "The Influence of Temperature on Bioflocculation and Settling of Activated Sludge and their Flocculation Mechanisms Involved." Advanced Materials Research 518-523 (May 2012): 1817–24. http://dx.doi.org/10.4028/www.scientific.net/amr.518-523.1817.

Full text

Abstract:

Effects of temperature (conventional (25°С) vs. mesophilic (35°С) vs. thermophilic (55°С)) on activated sludge properties (production and composition of EPS and interaction potential) and their roles in bioflocculation and settling were studied using well-controlled sequencing batch reactors fed with a synthetic wastewater comprised of glucose and inorganic nutrients. The results show that thermophilic sludge had a poorer bioflocculation ability and settleability than that of conventional and mesophilic sludge. Analysis of extracellular polymeric substances (EPS) indicates that thermophilic sl

APA, Harvard, Vancouver, ISO, and other styles

50

Zhou, Youfei, Weijie Hu, Jun Sheng, Cheng Peng, and Tianfeng Wang. "Comparison of Anaerobic Co-Digestion of Buffalo Manure and Excess Sludge with Different Mixing Ratios under Thermophilic and Mesophilic Conditions." Sustainability 15, no. 8 (2023): 6690. http://dx.doi.org/10.3390/su15086690.

Full text

Abstract:

In this study, the main aim is to evaluate the mixing ratio of co-digestion of buffalo manure (BM) and excess sludge (ES) influenced for methane yield and digestate dewaterability. Five batch experiments with different BM and ES mixing ratios were carried out under thermophilic and mesophilic conditions. The methane yield of co-digestion of BM and ES increased by 10.1–73.5% under thermophilic conditions and 87.9–153.3% under mesophilic conditions, compared with the mono-anaerobic digestion of ES under the same conditions. Shannon and Chao1 indices showed that the bacterial species of the mesop

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!