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Journal articles on the topic 'Thin layer Chromatography-Direct Bioautography'

Author: Grafiati

Published: 7 June 2025

Last updated: 2 August 2025

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1

Choma, I. M. "Thin‐Layer Chromatography‐Direct Bioautography of Flumequine Residues in Milk." Journal of Liquid Chromatography & Related Technologies 29, no. 14 (2006): 2083–93. http://dx.doi.org/10.1080/10826070600759942.

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Choma, Irena M., and Edyta M. Grzelak. "Bioautography detection in thin-layer chromatography." Journal of Chromatography A 1218, no. 19 (2011): 2684–91. http://dx.doi.org/10.1016/j.chroma.2010.12.069.

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3

Vishwa, Patel1* Niyati Acharya2. "Application of TLC Bioautography for Natural Bioactive Screening." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 1376–86. https://doi.org/10.5281/zenodo.15378848.

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Bioautography is a mean of targeted-directed isolation of active molecules on chromatogram. Bioautography is technique to detect substances affecting the growth rates to test organisms in complex mixtures and matrices. The antimicrobial, antioxidant and enzyme inhibitory activities can be performed on TLC bioautography. Bioautography methods are divided into three categories. 1)Agar diffusion or contact bioautography 2) Immersion or agar-overlay bioautography 3) Direct bioautography. High throughput method enabling analyses of many samples in parallel and the comparison of their activity. Both

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4

Anuar, Nadia, Sadia Sultan, and Kamran Ashraf. "An Overview of Antimicrobial and Antioxidant Bioautography Method Analysis: Cosmos caudatus and Orthosiphon stamineus." International Journal of Pharmaceuticals, Nutraceuticals and Cosmetic Science 5, no. 1 (2022): 1–12. http://dx.doi.org/10.24191/ijpnacs.v5i1.01.

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Medicinal herbs such as Cosmos caudatus and Orthosiphon stamineus are sources of a huge number of bioactive compounds with antioxidant and antimicrobial properties. C. caudatus plant has bioactive compounds such as phenolics, flavonoids, and terpenoids. On the other hand, phytochemicals from O. stamineus have been shown to have a wide range of traditional applications due to their antioxidant and antibacterial characteristics. The bioautography method is used to isolate active molecules on chromatogram followed by the chemical and biological detection system. In antimicrobial bioautography, ag

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Choma, Irena, Adam Choma, and Krystyna Staszczuk. "Direct bioautography-thin-layer chromatography of flumequine and doxycycline in milk." Journal of Planar Chromatography – Modern TLC 15, no. 3 (2002): 187–91. http://dx.doi.org/10.1556/jpc.15.2002.3.5.

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6

Choma, I. M., A. Choma, I. Komaniecka, K. Pilorz, and K. Staszczuk. "Semiquantitative Estimation of Enrofloxacin and Ciprofloxacin by Thin‐Layer Chromatography–Direct Bioautography." Journal of Liquid Chromatography & Related Technologies 27, no. 13 (2004): 2071–85. http://dx.doi.org/10.1081/jlc-120039419.

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7

Isnaeni, Isnaeni, Andri Astuti, and Muhammad Yuwono. "Validation of Thin-Layer Chromatography-Bioautographic Method for Determination of Streptomycin." JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA 4, no. 1 (2018): 34. http://dx.doi.org/10.20473/jfiki.v4i12017.34-38.

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Background: A simple bio-assay for determination of streptomycin hyphenated with planar chromatography techniques was developed. Objective: This study aims to validate the method for identification and determination of streptomycin in injection preparations with TLC-bioautography. Methods: Thin Layer Chromatography (TLC) was performed on the silica Gel GF-254 using KH2PO4 solution as mobile solvent. The visualization was performed by spraying 2% resorcinol. Direct bi autography was developed using Escherichia coli ATCC 25922 as a bacterial test, grown on the nutrient agar medium at 37oC for 24

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Balázs, Viktória Lilla, Barbara Horváth, Erika Kerekes, et al. "Anti-Haemophilus Activity of Selected Essential Oils Detected by TLC-Direct Bioautography and Biofilm Inhibition." Molecules 24, no. 18 (2019): 3301. http://dx.doi.org/10.3390/molecules24183301.

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Essential oils (EOs) are becoming increasingly popular in medical applications because of their antimicrobial effect. Direct bioautography (DB) combined with thin layer chromatography (TLC) is a screening method for the detection of antimicrobial compounds in plant extracts, for example, in EOs. Due to their lipophilic character, the common microbiological assays (etc. disk diffusion) could not provide reliable results. The aim of this study was the evaluation of antibacterial and anti-biofilm properties of the EO of cinnamon bark, clove, peppermint, thyme, and their main components against Ha

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9

Jesionek, Wioleta, Edyta Grzelak, Barbara Majer-Dziedzic, and Irena Choma. "Thin-layer chromatography — Direct bioautography for the screening of antimicrobial properties of plant extracts." Journal of Planar Chromatography – Modern TLC 26, no. 2 (2013): 109–13. http://dx.doi.org/10.1556/jpc.26.2013.2.1.

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10

Choma, I. M., A. Choma, and K. Staszczuk. "DETERMINATION OF FLUMEQUINE IN MILK BY THIN-LAYER CHROMATOGRAPHY-BIOAUTOGRAPHY." Journal of Liquid Chromatography & Related Technologies 25, no. 10-11 (2002): 1579–87. http://dx.doi.org/10.1081/jlc-120005705.

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11

Ramírez-Rueda, Román Yesid, Jane Marinho, and Marcos José Salvador. "Bioguided identification of antimicrobial compounds from Chrysopogon zizaniodes (L.) Roberty root essential oil." Future Microbiology 14, no. 14 (2019): 1179–89. http://dx.doi.org/10.2217/fmb-2019-0167.

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Aim: To determine the group of compounds from Chrysopogon zizaniodes root essential oil that have antimicrobial activity. Materials & methods: Thin-layer chromatography coupled to direct bioautography was used to determinate the fraction(s) having antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VREF). Through GC-MS identification, the fractions with the greatest similarity to the active thin-layer chromatography fraction were used to determinate the MIC. Results: The subfraction 8 from column chromatography w

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12

Wang, Meng, Yirong Zhang, Ruijie Wang, Zhibin Wang, Bingyou Yang, and Haixue Kuang. "An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography." Molecules 26, no. 15 (2021): 4647. http://dx.doi.org/10.3390/molecules26154647.

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Thin-layer chromatography (TLC) bioautography is an evolving technology that integrates the separation and analysis technology of TLC with biological activity detection technology, which has shown a steep rise in popularity over the past few decades. It connects TLC with convenient, economic and intuitive features and bioautography with high levels of sensitivity and specificity. In this study, we discuss the research progress of TLC bioautography and then establish a definite timeline to introduce it. This review summarizes known TLC bioautography types and practical applications for determin

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Rakotoniriana, Erick Francisco, Gabrielle Chataigné, Guy Raoelison, et al. "Characterization of an endophytic whorl-forming Streptomyces from Catharanthus roseus stems producing polyene macrolide antibiotic." Canadian Journal of Microbiology 58, no. 5 (2012): 617–27. http://dx.doi.org/10.1139/w2012-034.

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An endophytic whorl-forming Streptomyces sp. designated as TS3RO having antifungal activity against a large number of fungal pathogens, including Sclerotinia sclerotiorum , Rhizoctonia solani , Colletotrichum gloeosporioides , Cryphonectria parasitica , Fusarium oxysporum , Pyrenophora tritici-repentis , Epidermophyton floccosum , and Trichophyton rubrum , was isolated from surface-sterilized Catharanthus roseus stems. Preliminary identification showed that Streptomyces cinnamoneus subsp. sparsus was its closest related species. However, strain TS3RO could readily be distinguished from this sp

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14

Neidert, Eli, Peter W. Saschenbrecker, and Frank Tittiger. "Thin Layer Chromatographic/Bioautographic Method for Identification of Antibiotic Residues in Animal Tissues." Journal of AOAC INTERNATIONAL 70, no. 2 (1987): 197–200. http://dx.doi.org/10.1093/jaoac/70.2.197.

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Abstract An analytical method for the identification of the residues from 14 commonly used antibiotics is presented. The technique is based on selective tissue extraction followed by thin layer chromatography (TLC)/bioautography. Antibiotic residues are extracted from the tissues with methanol and methanol-HCl (98 + 2). The methanol extract is further extracted with chloroform to isolate groups of antibiotics. The extracts are spotted onto TLC plates and developed in suitable solvent systems. Developed plates are placed on set medium seeded with Bacillus subtilis and a bioautograph is produced

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15

Alimuddin, Andi Hairil, and Masriani Masriani. "ANTIMICROBIAL ACTIVITIES OF Shorea foxworthyi Sym STEAM BARK METHANOL EXTRACT." Indonesian Journal of Chemistry 8, no. 1 (2010): 114–18. http://dx.doi.org/10.22146/ijc.21656.

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Screening of antimicrobial activity compound from steam bark of Shorea foxworthyi Sym by Thin Layer Chromatography-Bioautography method have been conducted. The result of this research can be base in elucidation of antimicrobial activity compounds from S. foxworthyi Sym. The first step was done in this research that is maceration of S. foxworthyi steam bark using methanol solvent. Fractination to methanol extract was done using n-hexane, chloroform, and ethyl acetate solvent, respectively. Phytochemical screening were done to methanol, chloroform, n-hexane, and ethyl acetate fractions. Screeni

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Grzelak, Edyta M., Barbara Majer-Dziedzic, and Irena M. Choma. "Development of a Novel Direct Bioautography–Thin-Layer Chromatography Test: Optimization of Growth Conditions for Gram-Negative Bacteria, Escherichia coli." Journal of AOAC INTERNATIONAL 94, no. 5 (2011): 1567–72. http://dx.doi.org/10.5740/jaoac.10-385.

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Abstract With the aim of developing a TLC-direct bioautography assay using Escherichia coli as test bacteria, various parameters influencing the viability of microorganisms on TLC plates were examined and checked for flumequine standards. The optimal times for preincubation and incubation of bacterial broth were 20 h at 37°C and 2 h at 37°C, respectively. The optimal viscosity of the broth was obtained for 0.05% agarose solution in Mueller-Hinton broth. Various incubation times of the seeded TLC plates were also tested (5 h proved to be optimal). After incubation, the plates were sprayed with

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17

Móricz, Ágnes M., Györgyi Horváth, Andrea Böszörményi, and Péter G. Ott. "Detection and Identification of Antibacterial and Antioxidant Components of Essential Oils by TLC-Biodetection and GC-MS." Natural Product Communications 11, no. 11 (2016): 1934578X1601101. http://dx.doi.org/10.1177/1934578x1601101120.

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Components of cinnamon bark, rosemary, clove and thyme essential oils were screened for antioxidant and antibacterial activity utilizing thin-layer chromatography (TLC) coupled with the DPPH• test and direct bioautography using Bacillus subtilis cells. The compounds in the active chromatographic zones were identified by solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) after their elution. Seven antibacterial components were found: cinnamaldehyde and eugenol in cinnamon bark oil, 1,8-cineole, camphor, borneol and α-terpineol in rosemary oil, eugenol in clove oil and

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18

Jesionek, Wioleta, Irena Maria Choma, Barbara Majer-Dziedzic, and Irena Malinowska. "SCREENING BACTERIAL AND RADICAL SCAVENGING PROPERTIES OF CHOSEN PLANT EXTRACTS USING THIN-LAYER CHROMATOGRAPHY–DIRECT BIOAUTOGRAPHY." Journal of Liquid Chromatography & Related Technologies 37, no. 20 (2014): 2882–91. http://dx.doi.org/10.1080/10826076.2014.907103.

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19

Bories, Georges F., Maryse M. Baradat, and Denis E. Corpet. "Bioautographic Method for Evaluation of Glycopeptide Actaplanin in Milk." Journal of AOAC INTERNATIONAL 69, no. 6 (1986): 938–40. http://dx.doi.org/10.1093/jaoac/69.6.938.

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Abstract A bioautographic method is described that allows the identification and semiquantitation of residues of the glycopeptide antibiotic actaplanin in cow’s milk at 0.01 ppm. The milk sample is precipitated with acetonitrile and the resultant pellet is extracted by a buffer. This extract is defatted, then chromatographed on an Amberlite resin column. Actaplanin is eluted with methanol-HCI. Purified extract is then chromatographed on a cellulose thin layer, developed in a methanol-chloroform- ammonium hydroxide mixture. This thin layer chromatography increased the sensitivity of the determi

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20

Parveen, Rabea, Nausheen Khan, Sultan Zahiruddin, et al. "TLC-Bioautographic Evaluation for High-Throughput Screening and Identification of Free Radical Scavenging and Antidiabetic Compounds from Traditional Unani Medicinal Plant: Citrullus colocynthis Schrad." Journal of AOAC INTERNATIONAL 103, no. 3 (2020): 669–77. http://dx.doi.org/10.5740/jaoacint.19-0287.

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Abstract Background: Interest in the antioxidant and antidiabetic activity of natural products are growing vastly in the modern world. Thin layer chromatography-bioautography-mass spectroscopy (TLC-bioautography-MS) plays an important role in chemico-biological screening of natural sources. TLC combined with 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) free radical, α-amylase and α-glucosidase bioassay were used to evaluate antioxidant and antidiabetic activities, respectively, in different extracts of Citrullus colocynthis (Hanzal), a well-known traditional Indian Unani medicinal plant. Objective: T

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21

Tock, Margaux Lim Ah, Sandra Combrinck, Guy Kamatou, Weiyang Chen, Sandy Van Vuuren, and Alvaro Viljoen. "Antibacterial Screening, Biochemometric and Bioautographic Evaluation of the Non-Volatile Bioactive Components of Three Indigenous South African Salvia Species." Antibiotics 11, no. 7 (2022): 901. http://dx.doi.org/10.3390/antibiotics11070901.

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Salvia africana-lutea L., S. lanceolata L., and S. chamelaeagnea L. are used in South Africa as traditional medicines to treat infections. This paper describes an in-depth investigation into their antibacterial activities to identify bioactive compounds. Methanol extracts from 81 samples were screened against seven bacterial pathogens, using the microdilution assay. Biochemometric models were constructed using data derived from minimum inhibitory concentration (MIC) and ultra-performance liquid chromatography-mass spectrometry data. Active molecules in selected extracts were tentatively identi

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22

Jóźwiak, Grzegorz W., Barbara Majer-Dziedzic, Wioleta Jesionek, W. Zieliński, and Monika Waksmundzka-Hajnos. "Thin-layer chromatography: Direct bioautography as a method of examination of antimicrobial activity of selected Potentilla species." Journal of Liquid Chromatography & Related Technologies 39, no. 5-6 (2016): 281–85. http://dx.doi.org/10.1080/10826076.2016.1163466.

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23

Vanderkop, Perry A., and James D. Macneil. "Thin-Layer Chromatography/Bioautography Method for Detection of Monensin in Poultry Tissues." Journal of AOAC INTERNATIONAL 72, no. 5 (1989): 735–38. http://dx.doi.org/10.1093/jaoac/72.5.735.

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Abstract Monensin is an effective anticoccidial agent widely used in the poultry industry. Because of concerns over its toxicity, a sensitive, reliable, fast, and simple method for residue detection in poultry tissues is desirable from both a diagnostic and a regulatory view. Many methods of detection are excluded due to this ionophore's complex chemical and biological nature. A common method used for its detection is thin-layer chromatography/bioautography (TLC/B), although this is usually only semiquantitative. A new TLC/B method for monensin detection in poultry tissues was developed and is

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Grzelak, Edyta M., Barbara Majer-Dziedzic, Irena M. Choma, and Karol M. Pilorz. "Development of a Novel Direct Bioautography-Thin-Layer Chromatography Test: Optimization of Growth Conditions for Gram-Positive Bacteria, Bacillus subtilis." Journal of AOAC INTERNATIONAL 96, no. 2 (2013): 386–91. http://dx.doi.org/10.5740/jaoacint.11-466.

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Abstract A TLC-direct bioautography (DB) assay using Bacillus subtilis as test bacteria was developed. Various factors affecting the microorganism's viability on the TLC plates were studied and verified for the flumequine standards. The Dhenasar's method called "direct sample determination" was used for TLC; the antibiotic samples were spotted on the TLC plates and subjected to bioautography without developing with a mobile phase. The best preincubation and incubation times of bacterial broth were found to be 1 h at 37°C and 6 h at 37°C. The optimal viscosity of broth was obtained by the addit

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25

Valle, Demetrio L., Juliana Janet M. Puzon, Esperanza C. Cabrera, and Windell L. Rivera. "Thin Layer Chromatography-Bioautography and Gas Chromatography-Mass Spectrometry of Antimicrobial Leaf Extracts from PhilippinePiper betleL. against Multidrug-Resistant Bacteria." Evidence-Based Complementary and Alternative Medicine 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/4976791.

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This study isolated and identified the antimicrobial compounds of PhilippinePiper betleL. leaf ethanol extracts by thin layer chromatography- (TLC-) bioautography and gas chromatography-mass spectrometry (GC-MS). Initially, TLC separation of the leaf ethanol extracts provided a maximum of eight compounds withRfvalues of 0.92, 0.86, 0.76, 0.53, 0.40, 0.25, 0.13, and 0.013, best visualized when inspected under UV 366 nm. Agar-overlay bioautography of the isolated compounds demonstrated two spots withRfvalues of 0.86 and 0.13 showing inhibitory activities against two Gram-positive multidrug-resis

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RAMIREZ, A., R. GUTIERREZ, G. DIAZ, et al. "High-performance thin-layer chromatography–bioautography for multiple antibiotic residues in cow’s milk." Journal of Chromatography B 784, no. 2 (2003): 315–22. http://dx.doi.org/10.1016/s1570-0232(02)00819-x.

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27

Salisbury, Craig D. C., Charlotte E. Rigby, and Wayne Chan. "Determination of antibiotic residues in Canadian slaughter animals by thin-layer chromatography-bioautography." Journal of Agricultural and Food Chemistry 37, no. 1 (1989): 105–8. http://dx.doi.org/10.1021/jf00085a024.

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28

Vander Kop, Perry A., and James D. Mac Neil. "Separation and detection of monensin, lasalocid and salinomycin by thin-layer chromatography/bioautography." Journal of Chromatography A 508 (January 1990): 386–90. http://dx.doi.org/10.1016/s0021-9673(00)91282-2.

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29

Jesionek, Wioleta, Barbara Majer-Dziedzic, Györgyi Horváth, Ágnes M. Móricz, and Irena M. Choma. "Screening of antibacterial compounds in Thymus vulgaris L. tincture using thin-layer chromatography—direct bioautography and liquid chromatography—tandem mass spectrometry techniques." JPC - Journal of Planar Chromatography - Modern TLC 30, no. 2 (2017): 131–35. http://dx.doi.org/10.1556/1006.2017.30.2.7.

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Jesionek, Wioleta, Barbara Majer-Dziedzic, Györgyi Horváth, Ágnes M. Móricz, and Irena M. Choma. "Screening of antibacterial compounds in Salvia officinalis L. tincture using thin-layer chromatography—direct bioautography and liquid chromatography—tandem mass spectrometry techniques." JPC - Journal of Planar Chromatography - Modern TLC 30, no. 5 (2017): 357–62. http://dx.doi.org/10.1556/1006.2017.30.5.4.

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31

Piech, Tomasz, Barbara Majer-Dziedzic, Anna Kostruba, Edyta M. Grzelak, and Irena M. Choma. "Thin-layer chromatography—direct bioautography as an alternative method for screening of antibiotic residues in milk: A comparative study." Journal of Liquid Chromatography & Related Technologies 39, no. 5-6 (2016): 292–97. http://dx.doi.org/10.1080/10826076.2016.1163468.

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Jóźwiak, Grzegorz, Barbara Majer-Dziedzic, Justyna Kwiecińska, and Monika Waksmundzka-Hajnos. "Comparison of the microbiological activities of different varieties of hop (Humulus lupulus) extracts by thin-layer chromatography—direct bioautography." JPC - Journal of Planar Chromatography - Modern TLC 30, no. 2 (2017): 126–30. http://dx.doi.org/10.1556/1006.2017.30.2.6.

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Móricz, Ágnes M., Tim T. Häbe, Péter G. Ott, and Gertrud E. Morlock. "Comparison of high-performance thin-layer with overpressured layer chromatography combined with direct bioautography and direct analysis in real time mass spectrometry for tansy root." Journal of Chromatography A 1603 (October 2019): 355–60. http://dx.doi.org/10.1016/j.chroma.2019.03.068.

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Amountzias, Vaios, Evagelos Gikas, and Nektarios Aligiannis. "HPTLC Combined with sHetCA and Multivariate Statistics for the Detection of Bioactive Compounds in Complex Mixtures." Molecules 29, no. 24 (2024): 6027. https://doi.org/10.3390/molecules29246027.

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High-Performance Thin Layer Chromatography (HPTLC) is widely utilized in natural products research due to its simplicity, low cost, and short total analysis time, including data treatment. While bioautography can be used for rapid detection of bioactive compounds in extracts, the number of available bioautographic methods is limited mainly due to the high cost and difficulty in developing protocols that lead to accurate and reproducible results. For this reason, an alternative method for the detection of bioactive compounds in plant extracts prior to their isolation using HPTLC, combined with

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Zheng, Xi-yuan, Lei Zhang, Xue-mei Cheng, Zi-jia Zhang, Chang-hong Wang, and Zheng-tao Wang. "Identification of acetylcholinesterase inhibitors from seeds of plants of genusPeganumby thin-layer chromatography-bioautography." Journal of Planar Chromatography – Modern TLC 24, no. 6 (2011): 470–74. http://dx.doi.org/10.1556/jpc.24.2011.6.3.

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Yang, Yingbo, Lihua Gu, Ying Xiao та ін. "Rapid Identification of α-Glucosidase Inhibitors from Phlomis tuberosa by Sepbox Chromatography and Thin-Layer Chromatography Bioautography". PLOS ONE 10, № 2 (2015): e0116922. http://dx.doi.org/10.1371/journal.pone.0116922.

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37

Setiawan, Finna. "Antioxidant Activity and Phytochemical Profile in Sequential Solvent Extract of Faloak (Sterculia quadrifida) Leaves and Stem Bark." International Journal of Agriculture and Biology 33, no. 06 (2025): 330603. https://doi.org/10.17957/ijab/15.2323.

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Faloak (Sterculia quadrifida R.Br) is an endemic plant of East Nusa Tenggara Island. This plant was found in the East Nusa Tenggara archipelago, including Timor, Sumba, Flores, Alor and Rote Islands. Traditionally, the community used this plant for a variety of therapeutic purposes. Faloak demonstrate numerous biological activities, primarily attributable to its secondary metabolite compounds. The presence of phenolic compounds and flavonoids renders it a good candidate for the development of a novel natural antioxidant resource. Faloak possesses several biological activities related to its hi

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38

Sherma, Joseph. "Review of the Determination of the Antioxidant Activity of Foods, Food Ingredients, and Dietary Supplements by Thin Layer Chromatography-Direct Bioautography, Spectrometry, and the Dot-Blot Procedure." Journal of AOAC INTERNATIONAL 101, no. 5 (2018): 1285–94. http://dx.doi.org/10.5740/jaoacint.18-0116.

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Abstract This paper reviews a selection of the most important studies on antioxidants in foods (including beverages), food ingredients, and dietary supplements by effect-directed analysis using TLC with DPPH*, ABTS*+, and β-carotene direct bioautography. Total antioxidant activity by visible mode spectrometry (colorimetry) with TLC used offline to obtain additional analytical results, mostly for identification and quantification of phenolic compounds in samples, is also discussed. Finally, dot-blot assay for total antioxidant activity, carried out on a TLC plate without mobile-phase developmen

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Móricz, Ágnes M., and Péter G. Ott. "Separation and detection of apricot leaf triterpenes by high-performance thin-layer chromatography combined with direct bioautography and mass spectrometry." Journal of Chromatography A 1675 (July 2022): 463167. http://dx.doi.org/10.1016/j.chroma.2022.463167.

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40

Choma, Irena, and Iwona Komaniecka. "Matrix Solid‐Phase Dispersion Combined with Thin‐Layer Chromatography–Direct Bioautography for Determination of Enrofloxacin and Ciprofloxacin Residues in Milk." Journal of Liquid Chromatography & Related Technologies 28, no. 16 (2005): 2467–78. http://dx.doi.org/10.1080/10826070500189406.

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Darwish, Reham S., Eman Shawky, Hala M. Hammoda, and Fathallah M. Harraz. "A new thin-layer chromatography–direct bioautography assay for the qualitative and quantitative determination of peroxidase inhibitors in plant extracts." JPC – Journal of Planar Chromatography – Modern TLC 33, no. 1 (2020): 3–9. http://dx.doi.org/10.1007/s00764-019-00004-0.

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Prasetya, Fajar, M. Arifuddin, and Arsyik Ibrahim. "Identifikasi Senyawa Marker Dominan Ekstrak Daun Sirih Hitam dan Kuantifikasi Berdasarkan Perbedaan Lokasi Tanam." Jurnal Sains dan Kesehatan 3, no. 2 (2021): 147–57. http://dx.doi.org/10.25026/jsk.v3i2.386.

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It has been revealed that the benefits of black betel leaf extract have created an opportunity for the black betel plant to be commercialized. The several benefits of black betel leaf extract are antimicrobial, anti-inflammatory, cytotoxic, and antioxidant. The extraction method uses maceration techniques. Identification of active markers as antimicrobials (Streptococcus mutans, Streptococcus sanguinis, Candida albicans) begins with Bioautography Thin Layer Chromatography (TLC), for isolation from macerated extracts followed by Vacuum Liquid Chromatography Fractionation (KCV), conventional Col

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Muthia, Farah, Sukmawati Sukmawati, and Fitriana Fitriana. "Antibacterial Activity of Ethanol Extract of Qust Al Hindi Plant Root (Saussurea Lappa) Against Bacteria Staphylococcus aureus and Escherichia coli by TLC-Bioautography." Journal Microbiology Science 3, no. 2 (2023): 20–29. http://dx.doi.org/10.56711/jms.v3i2.980.

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The Indian Costus plant (Saussurea lappa) possesses the ability as an antibacterial, which contains alkaloid compounds, whereas this study aims to determine the antibacterial activity of ethanol extract of Indian Costus plant root (Saussurea lappa). The extraction process of Indian Costus plant root simplicia (Saussurea lappa) was carried out using the Soxhlet method with 96% ethanol as a solvent, and then evaporated with a rotavapor to obtain a viscous extract. Screening tests were carried out by giving ethanol extract of Indian Costus plant root (Saussurea lappa) with a concentration of 0.1%

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Petz, Michael, Roy Solly, Moira Lymburn, and Mike H. Clear. "Thin-Layer Chromatographic Determination of Erythromycin and Other Macrolide Antibiotics in Livestock Products." Journal of AOAC INTERNATIONAL 70, no. 4 (1987): 691–97. http://dx.doi.org/10.1093/jaoac/70.4.691.

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Abstract A method is described for determination of 4 macrolide antibiotics in livestock products. Erythromycin, tylosin, oleandomycin, and spiramycin were extracted from animal tissues, milk, and egg with acetonirrile at pH 8.5. Cleanup was done by adding sodium chloride and dichloromethane, evaporating the organic layer, and subsequent acid/ base partitioning. After the antibiotics were separated by thin-layer chromatography (TLC), they were reacted with xanthydrol and could be detected as purple spots down to 0.02 mg/kg without interference by other commonly used therapeutic drugs (23 were

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Guleria, Sanjay, Ashok Kumar, and Ashok Kumar Tiku. "Chemical Composition and Fungitoxic Activity of Essential Oil of Thuja orientalis L. Grown in the North-Western Himalaya." Zeitschrift für Naturforschung C 63, no. 3-4 (2008): 211–14. http://dx.doi.org/10.1515/znc-2008-3-409.

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The essential oil from fresh leaves of Thuja orientalis L. grown in the north-western Himalaya was isolated by means of hydrodistillation and analyzed by GC and GC/MS. Twentytwo compounds representing 94.0% of the total oil were identified. The leaf oil contained α- pinene (29.2%), Δ-3-carene (20.1%), α-cedrol (9.8%), caryophyllene (7.5%), α-humulene (5.6%), limonene (5.4%), α-terpinolene (3.8%) and α-terpinyl acetate (3.5%) as major constituents. The essential oil showed antifungal activity against Alternaria alternata in a direct bioautography assay. Two main bioactive compounds named as b1

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Pyka, Alina. "Detection Progress of Selected Drugs in TLC." BioMed Research International 2014 (2014): 1–19. http://dx.doi.org/10.1155/2014/732078.

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This entry describes applications of known indicators and dyes as new visualizing reagents and various visualizing systems as well as photocatalytic reactions and bioautography method for the detection of bioactive compounds including drugs and compounds isolated from herbal extracts. Broadening index, detection index, characteristics of densitometric band, modified contrast index, limit of detection, densitometric visualizing index, and linearity range of detected compounds were used for the evaluation of visualizing effects of applied visualizing reagents. It was shown that visualizing effec

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Sarmila, Sarmila, Abd Rahman Razak, and Jamaluddin Jamaluddin. "ANTIBACTERIAL ACTIVITY OF PATCHOULI OIL REFINERY WASTE AGAINTS STAPHYLOCOCCUS AUREUS BACTERIA." Journal Microbiology Science 2, no. 1 (2022): 1–8. http://dx.doi.org/10.56711/jms.v2i1.821.

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Patchouli oil refining waste is biomass or organic waste which has chemical contect so that it has antibacterial potential. This study aims to determine the antibacterial activity of patchouli oil refining waste against Staphylococcus aureus and knowing the groups of compounds identified as having antibacterial activity after the TLC Bioautography test was carried out. The sample was extracted using the maceration method with 96% ethanol solvent. Antibacterial activity testing was carried out with a concentration variant of 50%, 25% and 10% with the well method. Thin layer Chromatography (TLC)

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Tomashevich, N. S., T. M. Sidorova, V. V. Allahverdyan, and A. M. Asaturova. "The study of metabolites of new promising strains of bacterial‐antagonists of the genus <i>Bacillus</i> to increase the effectiveness of fungicidal biological products based on them." South of Russia: ecology, development 18, no. 2 (2023): 70–81. http://dx.doi.org/10.18470/1992-1098-2023-2-70-81.

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Aim. To study the metabolomic profile of bacterial strains of the genus Bacillus promising for the development of biofungicides using thin layer chromatography (TLC), bioautography and high performance liquid chromatography with mass spectrometry (HPLC‐MS).Materials and Methods. The objects of study are strains of bacteria of the genus Bacillus. Exometabolites were isolated from the liquid culture and their metabolomic profile was analyzed by TLC, bioautography, and HPLC‐MS.Results. By the method of bioautography with a test culture of the fungus F. oxysporum var. orthoceras BZR F6 metabolites

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Rakshith, Devaraju, Parthasarathy Santosh, Kustiariyah Tarman, Doddahosuru Gurudatt, and Sreedharamurthy Satish. "Dereplication strategy for antimicrobial metabolite using thin-layer chromatography-bioautography and LC-PDA-MS analysis." Journal of Planar Chromatography – Modern TLC 26, no. 6 (2013): 470–74. http://dx.doi.org/10.1556/jpc.26.2013.6.2.

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Czernicka, Lidia, Agnieszka Ludwiczuk, Edward Rój, Zbigniew Marzec, Agata Jarzab, and Wirginia Kukula-Koch. "Acetylcholinesterase Inhibitors among Zingiber officinale Terpenes—Extraction Conditions and Thin Layer Chromatography-Based Bioautography Studies." Molecules 25, no. 7 (2020): 1643. http://dx.doi.org/10.3390/molecules25071643.

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Although numerous studies have been conducted on ginger extracts and fractions, the data on the pharmacological activity of single constituents of Zingiber officinale are still insufficient. To assess the antidementia properties of the plant, a thin layer chromatography (TLC)-based bioautography acetylcholinesterase inhibitory assay was performed on the Zingiber officinale diethyl ether extract. It led to the recognition of three active inhibitors among volatile constituents of the plant: ar-curcumene (A), α-sesquiphellandrene (B) and a-zingiberene (C). The identification of the components was

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