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Journal articles on the topic 'Thin-Layer- Chromatography High accuracy'

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Published: 7 June 2025

Last updated: 2 August 2025

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1

Renger, Bernd. "Contemporary Thin-Layer Chromatography in Pharmaceutical Quality Control." Journal of AOAC INTERNATIONAL 81, no. 2 (1998): 333–40. http://dx.doi.org/10.1093/jaoac/81.2.333.

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abstract The progress in layers, instrumentation, and the development of automated scanning densitometers have led to a remarkable improvement of the features of thin-layer chromatography (TLC) and especially its high performance version (HPTLC). Contemporary TLC or HPTLC combines the techniques' inherent advantages which include high sample throughput, simultaneous processing of standards and samples, flexibility, easy postchro matographic visualization techniques, and the ability to handle complex or crude samples with minimum sample cleanup—with improved selectivity, sensitivity, and accura

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Yegorova, A., Yu Scrypynets, G. Fedosenko, G. Maltsev, and S. Kashutskiy. "Practical application of high-performance liquid chromatography and high-performance thin-layer chromatography in pharmaceutical analysis." Zurnal Hromatograficnogo tovaristva 21, no. 67 (2021): 46–55. http://dx.doi.org/10.15407/zht2021.67.046.

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Currently, chromatographic methods are widely used to control the quality of products in the pharmaceutical and food industries. This article is devoted to the systematization of the results of scientific research of the authors on the development, validation and application of a number of chromatographic methods in the creation of generic drugs, as well as in the analysis of medicinal plant materials. The developed methods are validated according to the following parameters: specificity, linearity, accuracy and limit of detection. The specificity of the methods is based on the ability to eval

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Komal N. Pawar, Komal N. Pawar, Sandhya P. Kadam Sandhya P. Kadam, and Dr VivekkumarK Redasani Dr.VivekkumarK.Redasani. "A Systematic Review on High Performance Thin Layer Chromatography (HPTLC)." International Journal of Pharmaceutical Research and Applications 10, no. 3 (2025): 402–16. https://doi.org/10.35629/4494-1003402416.

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High-Performance Thin Layer Chromatography (HPTLC) is a sophisticated analytical tool that has transformed the science of separation science through its greater sensitivity, resolution, and reproducibility over the conventional Thin Layer Chromatography (TLC). This systematic review delves into the concept, instrumentation, methodological innovation, and vast applications of HPTLC in pharmaceuticals, herbal drugs, food, forensic science, and biotechnology. The review highlights the most important innovations in automation, detection, derivatization, and hyphenation with mass spectrometry and F

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Yegorova, A., Yu Loskutova, G. Voitiuk, A. Maltsev, E. Malinka, and Yu Scrypynets. "Determination of curcumin in bouillon cubes by high-performance thin-layer chromatography." Zurnal Hromatograficnogo tovaristva 20, no. 66 (2021): 4–14. http://dx.doi.org/10.15407/zht2020.66.004.

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Currently, high-performance thin-layer chromatography (HPTLC) is widely used to control product quality in the pharmaceutical and food industries. In the manufacture of food products, the most important requirement is the control of the content of various additives (preservatives, dyes, antioxidants). For the first time, a method for determining curcumin by high-performance thin-layer chromatography in bouillon cubes "Gallina Blanca" was proposed. Detection was carried out by densitometric scanning using CAMAG equipment when measuring absorbance at a wavelength 265 nm. The method is based on d

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Dr., W.D.Sam Solomon, Priyadharshini.R, K.Hemamalini, S.Priyavagini, K.R.Anjana, and V.Karnan. "Estimation of Piracetam in Bulk and Formulation Using High Performance Thin-Layer Chromatography." International Journal of Innovative Science and Research Technology 8, no. 3 (2023): 2052–55. https://doi.org/10.5281/zenodo.7807241.

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A rapid, accurate and precise HPTLC method has been developed for the estimation of Piracetam in bulk and Pharmaceutical formulation. In this method standard and sample solutions of Piracetam were applied on pre-coated 6 x 10 silica gel 60F254 TLC plate, and developed using chloroform: methanol:glacial acetic acid: triethyl amine (16:4:0.2:0.2) as mobile phase. A Camag HPTLC system comprising of Camag Linomat -5-applicator, Camag twin trough chamber, Camag TLC-3 scanner was used for the analysis. The drugs on the plate were scanned at 254 nm. The dynamic linearity range was 2µl, 4µ

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Kowalczuk, Dorota. "Simultaneous High-Performance Thin-Layer Chromatography Densitometric Assay of Trandolapril and Verapamil in the Combination Preparation." Journal of AOAC INTERNATIONAL 88, no. 5 (2005): 1525–29. http://dx.doi.org/10.1093/jaoac/88.5.1525.

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Abstract A high-performance thin-layer chromatography (TLC) method coupled with densitometric analysis has been developed for simultaneous measurement of trandolapril (TRA) and verapamil (VER) in 2-component mixtures and in their combination capsules. The active substances were extracted from capsules withmethanol (mean recovery: 103.4% for TRA, 97.13% for VER) and chromatographed on TLC plates coated with silica gel 60 F254 in horizontal chambers with ethyl acetate–ethanol–acetic acid (8 + 2 + 0.5, v/v) mobile phase. Chromatographic separation of these components was followed by ultraviolet d

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Mrunal D. Thakare and M.D. Game. "Validated HPTLC method development, comparative evaluation and stability study for berberine in herbal preparations." GSC Biological and Pharmaceutical Sciences 27, no. 2 (2024): 281–96. http://dx.doi.org/10.30574/gscbps.2024.27.2.0186.

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The study focusing on the development and validation of a high-performance thin-layer chromatography (HPTLC) method for quantifying berberine in pharmaceutical formulations. Various chromatographic parameters were optimized to ensure precise and reproducible results, including mobile phase composition, linearity range, detection wavelength, and spot characteristics. The method demonstrated high accuracy and linearity within the concentration range of 5 - 35 µg/mL, with a determined Rf value of 0.52±0.02 for berberine. Additionally, stability studies revealed that berberine underwent degradatio

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Mrunal, D. Thakare, and Game M.D. "Validated HPTLC method development, comparative evaluation and stability study for berberine in herbal preparations." GSC Biological and Pharmaceutical Sciences 27, no. 2 (2024): 281–96. https://doi.org/10.5281/zenodo.12541039.

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The study focusing on the development and validation of a high-performance thin-layer chromatography (HPTLC) method for quantifying berberine in pharmaceutical formulations. Various chromatographic parameters were optimized to ensure precise and reproducible results, including mobile phase composition, linearity range, detection wavelength, and spot characteristics. The method demonstrated high accuracy and linearity within the concentration range of 5 - 35 µg/mL, with a determined Rf value of 0.52±0.02 for berberine. Additionally, stability studies revealed that berberine underwe

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Edward Raju Gope, Shaik Munnisha Begum, Pujitha Prayer Anisetti, et al. "A Review of Principles, Applications, and Recent Developments in HPTLC and HPLC." Journal of Pharma Insights and Research 2, no. 6 (2024): 056–64. https://doi.org/10.69613/315vge42.

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High-Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC) represent fundamental analytical techniques in modern chemical analysis. These sophisticated separation methods have revolutionized the field of analytical chemistry through their precision, reliability, and versatility. HPTLC, an enhancement of traditional thin-layer chromatography, offers advantages such as minimal sample preparation, simultaneous analysis of multiple samples, and cost-effectiveness. It has found extensive applications in pharmaceutical analysis, natural product research, and

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Upton, Roy T. "Use of High-Performance Thin Layer Chromatography by the American Herbal Pharmacopoeia." Journal of AOAC INTERNATIONAL 93, no. 5 (2010): 1349–54. http://dx.doi.org/10.1093/jaoac/93.5.1349.

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Abstract TLC characterizations are among the key identity tests in most pharmacopoeial monographs. Pharmacopoeial standards are typically used by industry as a basis for meeting QC requirements and current good manufacturing practices (cGMPs). TLC is a relatively low-cost, highly versatile tool for developing specifications for raw materials, as well as for the various preparations for which pharmacopoeial standards are created. In addition to its use in the development of identity tests, TLC is a valuable tool for screening plant samples that pharmacopoeias must review in the development of m

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Gackowski, Marcin, Marcin Koba, Katarzyna Mądra-Gackowska, Piotr Kośliński, and Stefan Kruszewski. "Recent Applications of High Performance Thin Layer Chromatography and Derivative Spectrophotometry in Pharmaceutical Analysis." Current Pharmaceutical Analysis 16, no. 6 (2020): 671–89. http://dx.doi.org/10.2174/1573412915666190226155149.

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At present, no one can imagine drug development, marketing and post-marketing without rigorous quality control at each stage. Only modern, selective, accurate and precise analytical methods for determination of active compounds, their degradation products and stability studies are able to assure the appropriate amount and purity of drugs administered every day to millions of patients all over the world. For routine control of drugs simple, economic, rapid and reliable methods are desirable. The major focus of current scrutiny is placed on high-performance thin layer chromatography and derivati

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Liu, Xing Rong, Jian Zheng, Dao Chen Zhu, and Yuan Mei Chen. "Determination of Puerarin and Daidzin from Pueraria Root Based on Chromatography." Advanced Materials Research 524-527 (May 2012): 2273–77. http://dx.doi.org/10.4028/www.scientific.net/amr.524-527.2273.

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Combine thin layer chromatography ( TLC ) with high performance liquid chromatography ( HPLC ) detection of kudzu root isoflavones, to establish a rapid and accurate method to determine the component content of Puerarin and daidzin in Radix Puerariae.Though study on the optimization analysis of Puerarin Content in Radix Puerariae and daidzin content chromatography in a variety of conditions, including TLC developing solvent and options of coloration, and HPLC chromatographic separation conditions and detection characteristics of TLC and HPL, to get the best Chromatographic separation condition

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KB, Premakumari, Meenashi Vanathi B, Ezhilarasan V, and Harshal Tare. "Development and Validation of HPTLC for the Determination of Gefitinib HCl in Tablet Dosage Form." INTERNATIONAL JOURNAL OF PHARMACEUTICAL QUALITY ASSURANCE 15, no. 02 (2024): 831–35. http://dx.doi.org/10.25258/ijpqa.15.2.45.

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A simple, high-performance thin-layer chromatography (HPTLC) method of gefitinib HCl in pharmaceutical formulation was developed. Separation was done on TLC aluminum plates precoated by silica gel 60GF254 (20 × 20 cm) with 200 μm layer depth. With UV detection set to 254nm, chromatography was conceded out by the mobile phase of dichloromethane and methanol (9:1, v/v). Linearity was found to range of 50 to 300 ng/spot and, the correlation coefficient was found to be 0.99 and the Rf value was found to be 0.46. The system’s suitability was considered to ascertain the performance of ascertaining q

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Cherviakova, L., T. Panchenko, and O. Borzykh. "Analytical control of active substances of the class of imidazolinones in herbicide formulations." Karantin i zahist roslin, no. 1 (March 10, 2021): 15–18. http://dx.doi.org/10.36495/2312-0614.2021.1.15-18.

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Goal. The choice of a chromatographic method and the development of a method for the analytical determination of imazethapyr, imazamox, imazapyr in herbicide preparative forms in the joint presence. Methods. Active substances were analyzed by thin layer chromatography. The linear range of the dependence of the area of chromatographic zones on the amount of active substance was assessed by a mathematical-statistical method. Results. Determination of pesticides by thin-layer chromatography includes the main stages: classification of pesticides by polarity; extraction of active substa

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Pagadipally Santhosh Kumar, Pagadipally Santhosh Kumar, PatlollaNikitha Reddy PatlollaNikitha Reddy, Nagireddy Roshitha Nagireddy Roshitha, Anumolu Durga Pani Kumar Anumolu Durga Pani Kumar, and Naraparaju Swathi Naraparaju Swathi. "A comprehensive review on analytical methods of Cabotegravir." International Journal of Pharmaceutical Research and Applications 10, no. 2 (2025): 1784–88. https://doi.org/10.35629/4494-100217841788.

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HIV weakens the immune system, but highly active antiretroviral therapy (HAART), including the FDA-approved Cabotegravir, has improved life expectancy for those living with HIV. However, these treatments require lifelong use and can have side effects that impact adherence. Reliable methods to detect and measure Cabotegravir in pharmaceutical formulations and biological samples are crucial for ensuring its effectiveness. Chromatographic techniques like HighPerformance Liquid Chromatography (HPLC), Reverse-Phase HPLC (RP-HPLC), UltraPerformance Liquid Chromatography (UPLC), and High-Performance

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Kalyani, Ashok Patil*1 Shailesh B. Patil2. "To Develop And Validate Stability Indicating Assay Of Diloxanide Furoate By Using UV And HPLC And HPTLC Method For Pharmaceutical Dosage Form." International Journal in Pharmaceutical Sciences 2, no. 6 (2024): 498–517. https://doi.org/10.5281/zenodo.11543189.

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The study aimed to establish stability assays for Diloxanide Furoate using both High-Performance Liquid Chromatography (HPLC) and High-Performance Thin-Layer Chromatography (HPTLC) methods, covering crucial aspects of method development and validation. Detailed specifications were provided for the HPLC and HPTLC systems to ensure proper setup and functioning. Chromatographic conditions were optimized, including parameters like stationary phase, mobile phase composition, flow rate, and detection wavelength, to achieve optimal separation and detection of Diloxanide Furoate. The identity of pure

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Ahmad, Sufiyan, Mahendra Ganbase, Nitin Deshmukh та ін. "Development of validated HPTLC and UHPLC methods for quantiﬁcation of Withanolide in extract and formulation". International Journal of Experimental Research and Review 30 (1 травня 2023): 375–87. http://dx.doi.org/10.52756/ijerr.2023.v30.035.

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High performance thin layer chromatography (HPTLC) and Ultra-High-Performance Liquid Chromatography (UHPLC) techniques were developed and validated to quantify Withanolide in extract and formulation. On Al-backed silica gel 60 F254 TLC plates (10 cm × 10 cm, layer thickness 0.2 mm), which had been prewashed with methanol, HPTLC separation was carried out. Dichloromethane: Methanol: Toluene: Acetone in various ratios produced good separation in mobile phase (5:1:1:0.5 v/v). Camag TLC scanner densitometric scanning at 365 nm determined and quantified. This approach produced compact Withanolide s

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Gray, Dean E., Dale Messer, Andrew Porter, et al. "Analysis of Flavonol Aglycones and Terpenelactones in Ginkgo biloba Extract: A Comparison of High-Performance Thin-Layer Chromatography and Column High-Performance Liquid Chromatography." Journal of AOAC INTERNATIONAL 90, no. 5 (2007): 1203–9. http://dx.doi.org/10.1093/jaoac/90.5.1203.

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Abstract Advancements in automated high-performance thin-layer chromatography (HPTLC) have made it feasible to assess its use for the quantitative analysis of marker compounds in botanical preparations. We report here the findings of method comparisons for the terpenelactones and flavonol aglycones by column high-performance liquid chromatography (HPLC) with evaporative light scattering and UV detection, and HPTLC with a scanning densitometer. For the HPTLC assay of terpenelactones, total bilobalide, ginkgolide A, and ginkgolide B consistently achieved &lt;70 of the total determined using

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K, Suresh Kumar1* M. Sajin Arokia Raj2 R. Arivukkarasu3 N. Kiruthiga4 S. Dhinesh Kumar5. "Method Development And Validation Of Imeglimin Hydrochloride Using High-Performance Thin-Layer Chromatography In Bulk And Tablet Dosage Form." International Journal in Pharmaceutical Sciences 2, no. 8 (2024): 3445–53. https://doi.org/10.5281/zenodo.13347039.

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A novel method for analyzing Imeglimin Hydrochloride, an oral antidiabetic agent has been developed and validated using High-Performance Thin Layer Chromatography (HPTLC) for both bulk and tablet forms. The method utilizes a mobile phase of acetone, methanol, toluene, and formic acid in specific proportions. Chromatographic separation was achieved on silica gel TLC plates with densitometry scanning at 244 nm, where the drug shows significant absorbance. Validation followed ICH Q2R1 guidelines, demonstrating satisfactory results for Linearity, Accuracy, Precision (intra- and inter-day), Limit o

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Abdel-Kader, Maged S., Ayman A. Salkini, Prawez Alam, Khaled A. Alshahrani, Ahmed I. Foudah, and Mohammed H. Alqarni. "A High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Curcumin I, Curcumin II and Curcumin III in Curcuma longa and Herbal Formulation." Separations 9, no. 4 (2022): 94. http://dx.doi.org/10.3390/separations9040094.

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Curcuma longa (turmeric) has traditionally been used in Ayurvedic, Unani and herbal drugs to cure numerous ailments. Due to the high demand, the quantitative standardization of herbal products is challenging to maintain their quality. We aim to develop a rapid, sensitive and validated high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination and quantification of curcumin I, curcumin II and curcumin III in C. longa and herbal formulation. The three standards were separated using centrifugal preparative thin-layer chromatography (CPTLC) silica gel and identifi

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V.A., Ghodake B.A.Agarwal A.R. Tekade*. "STABILITY –INDICATING DENSITOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF DONEPEZIL HYDROCHLORIDE AND CURCUMIN IN INSITU NASAL GEL." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 05 (2018): 4097–106. https://doi.org/10.5281/zenodo.1251949.

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A stability indicating high performance thin layer chromatography (HPTLC) method was developed and validated for determination of Donepezil hydrochloride and curcumin in in situ nasal gel. Study was performed on pre-coated silica gel HPTLC plates using toluene: methanol: glacial acetic acid (8: 2: 0.1 v/v/v) as the mobile phase. A TLC scanner set at 254 nm was used for direct evaluation of the chromatograms in the reflectance/absorbance mode. Method was validated according to ICH guidelines. The correlation coefficients of calibration curves were found to be 0.994 and 0.988 in the concentratio

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Yue, Yongde, Rong Zhang, Wei Fan, and Feng Tang. "High-Performance Thin-Layer Chromatographic Analysis of Selected Organophosphorous Pesticide Residues in Tea." Journal of AOAC INTERNATIONAL 91, no. 5 (2008): 1210–17. http://dx.doi.org/10.1093/jaoac/91.5.1210.

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Abstract The separation of 9 organophosphates (monocrotophos, quinalphos, triazophos, parathion-methyl, isofenphos-methyl, temephos, parathion, phoxim-ethyl, and chlorpyrifos) by high-performance thin-layer chromatography (HPTLC) with automated multiple development was studied. The HPTLC method was developed and validated for analysis of residues of phoxim-ethyl and chlorpyrifos in tea. The sample was extracted with acetonitrile and cleaned up by ENVI-CARB solid-phase extraction. The extract was directly applied as bands to glass-backed silica gel 60F254 HPTLC plates. The plates were developed

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Heta, Kachhiya, Tandel Jinal, Rupchandani Isha, et al. "Development and Validation of HPTLC Method for Simultaneous Estimation of Azelnidipine and Telmisartan." Journal of Advances in Drug Discovery and Development 1, no. 1 (2023): 28–38. https://doi.org/10.5281/zenodo.8001476.

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<em>HPTLC method for simultaneous estimation of Azelnidipine and Telmisartan in bulk and their pharmaceutical dosage form was developed and validated. Pre coated silica gel G60-F<sub>254 </sub>aluminium sheet as a stationary phase and Toluene: Acetonitrile: Formic acid (5:4.5:0.5 % V/V/V) as mobile phase was optimized to develop chromatograph.  The method shown linearity in the range of 200-700 ng/ band for Azelnidipine and 1000-3500 ng/band for Telmisartan with R<sup>2</sup> 0.9961 and 0.9971 for Azelnidipine and Telmisartan respectively. %Recovery of 100.1 to 103.8% for Azelnidipine and

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Patel, V. B., J. S. Patel, and D. A. Shah. "ESTIMATION OF METFORMIN HYDROCHLORIDE AND TENELIGLIPTIN IN PHARMACEUTICAL FORMULATION BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY." INDIAN DRUGS 55, no. 10 (2018): 34–39. http://dx.doi.org/10.53879/id.55.10.11059.

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A new, accurate and precise high performance thin layer chromatographic (HPTLC) method has been developed for simultaneous analysis of metformin hydrochloride and teneligliptin in tablet formulation. The chromatographic separations were achieved on TLC plates precoated with silica gel G60 F254 and the plates were developed with 0.5% ammonium acetate-n propanol-ammonia (4.5:5:0.5, v/v/v) as mobile phase. Detection and evaluation of chromatograms were performed densitometrically at 252 nm. The retention factor of metformin hydrochloride and teneligliptin was found to be 0.2 and 0.63, respectivel

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Kumar Rout, Kedar, Sagarika Parida, and Sagar Kumar Mishra. "Standardization of the Ayurvedic Formulation Haridra Khanda Using High-Performance Thin-Layer Chromatography-Densitometry." Journal of AOAC INTERNATIONAL 91, no. 5 (2008): 1162–68. http://dx.doi.org/10.1093/jaoac/91.5.1162.

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Abstract The present study aimed to standardize the Ayurvedic preparation Haridra Khanda containing Curcuma longa as a major ingredient. Various physicochemical parameters such as alcohol-soluble extractive, water-soluble extractive, total ash, and acid-insoluble ash were determined according to the Ayurvedic Pharmacopoeia of India. Microscopic evaluation of the formulation revealed the presence of various diagnostic cell structures of C. longa. Trace metal analysis indicated the absence of toxic metals such as As, Cd, Hg, and Pb. High-performance thin-layer chromatographic (HPTLC) fingerprint

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Duffau, Boris E., Sonia Rojas, Lorena A. Delgado, and Sebastián Jofré. "High Performance Thin Layer Chromatography method for analysis of 3,4-methylenedioxymethamphetamine in seized tablets." Journal of Pharmacy & Pharmacognosy Research 3, no. 1 (2015): 162–70. http://dx.doi.org/10.56499/jppres15.096_3.6.162.

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Context: Consumption of synthetic drugs had increased in recent years, used as a recreational drug by young people who presume that consumption of this drug is harmless for health; however clinical studies have shown that this stimulant and its metabolites are toxic. Due to these reasons, chemical analysis of this illicit drug is crucial from the points of view of occupational medicine, toxicology, and law enforcement with the aim of pursuit the traffic of illegal drug. Aims: Implement and fully validate a rapid and simple method for detection and quantitation of MDMA by High-Performance Thin

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Li, Tao, Runtao Tian, Xinlan Yu, et al. "Application of Chemometric Algorithms in the High-Performance Thin-Layer Chromatography Fingerprint of Traditional Chinese Medicines." Journal of AOAC INTERNATIONAL 102, no. 3 (2019): 720–25. http://dx.doi.org/10.5740/jaoacint.18-0306.

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Abstract Background: The use of HPTLC fingerprinting for the analysis of traditional Chinese medicines (TCMs) usually involves several image-processing steps. However, these image-processing steps are time consuming. Objective: We describe a new approach that applies artificial neural networks (ANN) directly to raw high-performance thin-layer chromatography HPTLC images. Methods: This approach combines image processing and chemometric modeling and was used to classify TCMs [dried tangerine eel (Chen Pi), green tangerine peel (Qing Pi), immature bitter orange fruit, and bitter orange fruit (Zhi

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Patel, P. J., D. A. Shah, F. A. Mehta, and U. K. Chhalotiya. "DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM." INDIAN DRUGS 52, no. 12 (2015): 42–48. http://dx.doi.org/10.53879/id.52.12.10321.

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A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for

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Fortes, Paulo Jordão de Oliveira Cerqueira, Nelson Leal Alencar, Livio Cesar Cunha Nunes, et al. "CANNABIS THIN LAYER CHROMATOGRAPHY (TLC) CHEMO TYPING: HOW ACCURATE IS TLC COMPARED TO HIGH PRESSURE LIQUID CHROMATOGRAPHY?" Revista Contemporânea 3, no. 10 (2023): 19702–24. http://dx.doi.org/10.56083/rcv3n10-164.

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Problem: Brazilian Cannabis Associations (BCA) search for methods of reducing their costs and increasing their quality control of Cannabis spp. Oil and extracts. The significant difficulties presented by ABRACAM (Associação Brasileira Cannabis Medicinal) to UFPI professors were to standardize cannabinoids profiles of its plants and guarantee the same levels of cannabinoids on its artisanal extracts. ABRACAM has difficulty standardizing plant cannabinoids profiles due to Cannabis spp. Phenotypic plasticity, or the ability of Cannabis spp. to change attributes like the size of leaves, levels of

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Chirag V. Joshi, Chirag V. Joshi, Dhrumil H. Patel Dhrumil H. Patel, Khilan R. Patel Khilan R. Patel, et al. "A Comprehensive Review on Analytical Method Development and Validation for Linezolid in Pharmaceutical Dosage Forms." International Journal of Pharmaceutical Research and Applications 10, no. 2 (2025): 588–91. https://doi.org/10.35629/4494-1002588591.

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Linezolid, a synthetic oxazolidione antibiotic, is widely used to treat infections caused by Grampositive bacteria, including those resistant to other antibiotics. The development and validation of analytical methods for the determination of Linezolid in pharmaceutical dosage forms are critical for ensuring drug quality, efficacy, and safety. This review provides a comprehensive overview of the various analytical techniques employed for Linezolid quantification, including UV-Visible spectrophotometry, high-performance liquid Chromatography (HPLC), and high-performance thin-layer chromatography

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Sanyal, Asis K., Bhabadeb Chowdhury, and Arun B. Banerjee. "Rapid Thin-Layer Chromatographic Microestimation of Elemental Sulfur: Application to Complex Sulfur Ointments." Journal of AOAC INTERNATIONAL 76, no. 5 (1993): 1152–55. http://dx.doi.org/10.1093/jaoac/76.5.1152.

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Abstract Because of its nonpolar character, elemental sulfur can be separated easily from the components of complex samples and quantitated by thin-layer chromatography. Sulfur was separated by petroleum ether on silica gel G layers. The sulfur band on the chromatogram was scraped off and eluted with chloroform, and sulfur was estimated by its absorbance at 265 nm. The present method allows rapid, simple, but highly sensitive detection of sulfur down to 1 μg on the plate. The method was applied with high accuracy and precision to the analysis of complex sulfur ointments. No interference was en

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Selvadurai, Muralidharan, and Subramaniya Nainar Meyyanathan. "Sensitive and accurate estimation of losartan potassium formulation by high-performance thin-layer chromatography." Pharmaceutical Methods 2, no. 2 (2011): 95–98. http://dx.doi.org/10.4103/2229-4708.84444.

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Krzek, Jan, Urszula Hubicka, and Justyna Szczepańczyk. "High-Performance Thin-Layer Chromatography with Densitometry for the Determination of Ciprofloxacin and Impurities in Drugs." Journal of AOAC INTERNATIONAL 88, no. 5 (2005): 1530–36. http://dx.doi.org/10.1093/jaoac/88.5.1530.

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Abstract A thin-layer chromatographic (TLC)-densitometric method has been developed for identification and quantification of ciprofloxacin (Rf = 0.61) and an ethylenediamine compound (Rf = 0.42), a desfluoro compound (Rf = 0.48), by-compound A (Rf = 0.53), and fluoroquinolonic acid (Rf = 0.68) as ciprofloxacin degradation products in pharmaceutical preparations. By using chloroform–methanol–25% ammonia (43 + 43 + 14, v/v/v) as the mobile phase and silica gel 60 F254 high-performance TLC plates as the stationary phase, it was possible to separate individual constituents that, when subjected to

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Sai Chebrolu, Tejasvini, Lalit Kumar, and Ruchi Verma. "Lacidipine: review of analytical methods developed for pharmaceutical dosage forms and biological fluids." Bioanalysis 13, no. 12 (2021): 1011–24. http://dx.doi.org/10.4155/bio-2021-0024.

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Lacidipine (LAC) is a calcium antagonist used in the treatment of hypertension. It is a lipophilic drug containing dihydropyridine ring that is responsible for the activity. This review article gives an overview of various analytical techniques proposed for the determination of LAC in pharmaceutical dosage forms, in pure form, in biological fluids and to determine characteristics of LAC in modified release dosage forms. Ultra violet/visible spectrophotometric, spectroflourimetric, high performance liquid chromatography, high performance thin layer chromatography, electro-analytical, bioanalyti

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Bodart, Patricia, Charles Kabengera, Alfred Noirfalise, Philippe Hubert та Luc Angenot. "Determination of α-Solanine and α-Chaconine in Potatoes by High-Performance Thin-Layer Chromatography/Densitometry". Journal of AOAC INTERNATIONAL 83, № 6 (2000): 1468–73. http://dx.doi.org/10.1093/jaoac/83.6.1468.

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Abstract A high-performance thin-layer chromatographic (HPTLC) method was used to determine the glycoalkaloids α-solanine and α-chaconine in potatoes. α-Solanine and α-chaconine are extracted from dehydrated potatoes with boiling methanol–acetic acid (95 + 5, v/v). The analytes are separated on a Silica Gel 60 F254 HPTLC plate by a saturated mixture of dichloromethane–methanol–water–concentrated ammonium hydroxide (70 + 30 + 4 + 0.4, v/v), which is used for vertical development of the plate up to a distance of 85 mm. For visualization, the plate is dipped 3 times into a modified Carr-Price rea

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Philomindoss, Pratheema. "IDENTIFICATION, QUANTIFICATION AND VALIDATION OF STIGMASTEROL FROM ALPINIA CALCARATA USING HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 9 (2017): 126. http://dx.doi.org/10.22159/ijpps.2017v9i9.19988.

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Objective: The present study is designed to develop a new simple, precise, rapid and selective high‐performance thin‐layer chromatographic (HPTLC) method for the determination of stigmasterol in methanolic rhizomes extract of Alpinia calcarata.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of stigmasterol as standard on HPTLC plates for the quantification of stigmasterol from the Alpinia calcarata rhizomes. The concentration of standard stigmasterol is 1 mg/ml.Results: The retention factor of stigmasterol was 0.58. Linearity

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Kansara, Devansh, Usmangani K. Chhalotiya, Hetaben M. Kachhiya, and Ishita Patel. "Development of TLC method for simultaneous estimation of novel combination of amlodipine besylate, rosuvastatin calcium, and fimasartan potassium in synthetic mixture." Journal of Chemical Metrology 14, no. 2 (2020): 142–52. http://dx.doi.org/10.25135/jcm.45.20.07.1744.

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An accurate, sensitive, robust and precise high performance thin layer liquid chromatography method was developed based on ICH Q2 (R1) guidelines for estimation of novel combination of Amlodipine besylate, Rosuvastatin calcium and Fimasartan potassium in bulk and its synthetic mixture. Pre-coated silica gel aluminum plate 60 F254 was selected as the stationary phase and n-hexane, n-butanol, methanol, and Glacial Acetic Acid (5.7:2:2.3:0.1, v/v/v/v) was selected as mobile phase. All three drugs showing appreciable absorbance at the common wavelength of 242 nm were selected for quantification of

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Shunmuganathan, Elakkiya L., F. A. Mehta, and U. K. Chhalotiya. "STABILITY INDICATING HPTLC METHOD FOR THE ESTIMATION OF TICAGRELOR IN BULK AND IN PHARMACEUTICAL DOSAGE FORM." INDIAN DRUGS 53, no. 10 (2016): 34–39. http://dx.doi.org/10.53879/id.53.10.10506.

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A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of Ticagrelor in pharmaceutical dosage form. The method employed Thin-layer chromatography (TLC) aluminum plates precoated with silica gel 60 F254 as the stationary phase, while the solvent system was found to be toluene: ethyl acetate: acetic acid (5:4:1V/V/V). The Rf value was observed to be 0.33± 0.008. The spot was densitometrically analyzed in absorbance mode at 305 nm. The method was linear in the range of 50-250 ng/ band for ticagrelor. The limit of detection for tic

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Chervyakova, L., T. Panchenko, and O. Tsurkan. "Features of analytical control of glyphosate salts in preparative formulations." Interdepartmental Thematic Scientific Collection of Phytosanitary safety, no. 69 (March 14, 2024): 283–91. https://doi.org/10.36495/phss.2023.69.283-291.

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Goal. Development of a method of analytical control of glyphosate in preparative forms of herbicides. Methods. Determination of glyphosate was carried out by the chromatographic method of thin-layer chromatography (TLC) using Sorbfil plates with a thin layer of silica gel KSK adsorbent. The active substance was identified by the value of Rf, quantitative determination was based on the formalized dependence of the size of the area of the chromatographic zones on the amount of glyphosate. Results. Physicochemical properties of herbicides — electrolytes, to which glyphosate belongs, create specia

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Chervyakova, L., T. Panchenko, and O. Tsurkan. "Features of analytical control of glyphosate salts in preparative formulations." Interdepartmental Thematic Scientific Collection of Phytosanitary safety, no. 69 (March 14, 2024): 283–91. http://dx.doi.org/10.36495/1606-9773.2023.69.283-291.

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Goal. Development of a method of analytical control of glyphosate in preparative forms of herbicides. Methods. Determination of glyphosate was carried out by the chromatographic method of thin-layer chromatography (TLC) using Sorbfil plates with a thin layer of silica gel KSK adsorbent. The active substance was identified by the value of Rf, quantitative determination was based on the formalized dependence of the size of the area of the chromatographic zones on the amount of glyphosate. Results. Physicochemical properties of herbicides — electrolytes, to which glyphosate belongs, c

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Neamţu, Andreea Silvia, Andrei Biţă, Ion Romulus Scorei, et al. "Simultaneous quantitation of nicotinamide riboside and nicotinamide in dietary supplements via HPTLC–UV with confirmation by online HPTLC–ESI–MS." Acta Chromatographica 32, no. 2 (2020): 128–33. http://dx.doi.org/10.1556/1326.2019.00600.

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The identification and quantitation of nicotinamide riboside (NAR) and its main related compound (nicotinamide) were achieved using high-performance thin-layer chromatography (HPTLC)–ultraviolet (UV) densitometry with confirmation by online electrospray ionization (ESI)–mass spectrometry (MS). As the stationary phase, HPTLC Si 60 F254 glass plates were employed; the mobile phase was ethanol–1 M ammonium acetate–formic acid (7:1:0.1, v/v/v). No derivatization was applied, and UV densitometry was performed in the absorbance mode (270 nm). The method was validated by specificity, linearity, accur

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Nalbantova, Vanya, Velislava Todorova, Niko Benbassat, and Cedric Delattre. "Fenugreek galactomannan: High-Performance Thin-Layer Chromatography (HPTLC) method for identification and quantification of galactose and mannose." Pharmacia 71 (February 23, 2024): 1–8. http://dx.doi.org/10.3897/pharmacia.71.e118029.

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Fenugreek (Trigonella foenum-graecum L.) is a leguminous, medicinal plant that finds applications in traditional medicine and as an agent in pharmaceutical and nutraceutical products. Its seeds contain various compounds to which numerous beneficial effects are attributed, such as soluble polysaccharides, in particular galactomannan. There are limited data reporting the determination of Bulgarian fenugreek galactomannan composition by HPTLC. The analysis was successful in separating galactose and mannose, each demonstrating different Rf values 0.36, 0.48, respectively. After developing the meth

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Nalbantova, Vanya, Velislava Todorova, Niko Benbassat, and Cedric Delattre. "Fenugreek galactomannan: High-Performance Thin-Layer Chromatography (HPTLC) method for identification and quantification of galactose and mannose." Pharmacia 71, no. () (2024): 1–8. https://doi.org/10.3897/pharmacia.71.e118029.

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Fenugreek (<i>Trigonella foenum-graecum</i> L.) is a leguminous, medicinal plant that finds applications in traditional medicine and as an agent in pharmaceutical and nutraceutical products. Its seeds contain various compounds to which numerous beneficial effects are attributed, such as soluble polysaccharides, in particular galactomannan. There are limited data reporting the determination of Bulgarian fenugreek galactomannan composition by HPTLC. The analysis was successful in separating galactose and mannose, each demonstrating different Rf values 0.36, 0.48, respectively. After developing t

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Raviraj, Pawar* Vaibhav Paymal Jaya Kambale Dr. Nilesh Chougule. "Various Methods of Phytoconstituent Estimation." International Journal of Pharmaceutical Sciences 2, no. 11 (2024): 1445–50. https://doi.org/10.5281/zenodo.14224839.

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This review explores the diverse analytical methods used to assess phytoconstituents in medicinal plants, focusing on their unique   characteristics and therapeutic potential. Key techniques such as UV-VIS spectroscopy, Paper, thin-layer, high-performance liquid, and gas chromatography are examples of chromatography, and various spectroscopic methods (FTIR and mass spectrometry) are highlighted for their roles in the detection, isolation, and measurement of bioactive substances. The efficacy of these methods in evaluating antioxidant properties and quality control in phytopharmaceuti

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Vijaykumar K. Parmar, Deepika Mohanta, and Harsh Shah. "A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Berberine Chloride and Guggulsterone Z in Herbal Formulation." Journal of Pharmaceutical Technology, Research and Management 7, no. 1 (2019): 7–13. http://dx.doi.org/10.15415/jptrm.2019.71002.

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A simple, precise, and robust high-performance thin layer chromatography (HPTLC) method was developed and validated for the determination of berberine chloride and guggulsterone Z in herbal formulation. Chromatographic separation was achieved on aluminium plates precoated with silica gel G60F254 as the stationary phase and toluene-acetonitrile-formic acid (5:3:0.5 v/v/v) as the mobile phase. Densitometric evaluation was carried out at 264 nm. The present method was validated according to ICH guidelines. The Rf value of berberine chloride and guggulsterone Z was found to be 0.40 ± 0.02 and 0.68

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Pulpati, Harsha, Yogesh S. Biradar, and Mandapati Rajani. "High-Performance Thin-Layer Chromatography Densitometric Method for the Quantification of Harmine, Harmaline, Vasicine, and Vasicinone in Peganum harmala." Journal of AOAC INTERNATIONAL 91, no. 5 (2008): 1179–85. http://dx.doi.org/10.1093/jaoac/91.5.1179.

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Abstract Peganum harmala Linn. seed is a reputed drug of the Indian systems of medicine. We report a simple high-performance thin-layer chromatography (HPTLC) densitometric method for the quantification of 4 alkaloids, viz., harmine, harmaline, vasicine, and vasicinone from P. harmala seed. The 4 compounds were resolved in ethyl acetatemethanolammonia (7 1 0.3, v/v/v) mobile phase. The method was validated for precision, repeatability, and accuracy. Instrumental precision was 0.27, 1.53, 0.39, and 1.15% [relative standard deviation (RSD)] and repeatability of the method was 1.01, 0.79, 0.13, a

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Marciniec, Barbara, Magdalena Ogrodowczyk, and Anna Kwiecien′. "Effect of Radiation Sterilization on Alprenolol in the Solid State Studied by High-Performance Thin-Layer Chromatography." Journal of AOAC INTERNATIONAL 93, no. 3 (2010): 792–97. http://dx.doi.org/10.1093/jaoac/93.3.792.

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Abstract The possibility of radiation sterilization of alprenolol (AL) has been studied. Irradiation of AL in solid form with a 25 kGy beam of electrons caused only an insignificant change in color that became more intense with increasing irradiation dose. Moreover, with increasing dose a decrease in pH, the content of water, and the degree of crystallinity were observed. AL in solid form was radiated with a high-energy electron beam (9.96 MeV) at doses from 25400 kGy and analyzed by HPTLC using the mobile phase methanolammonia 25 (99 + 1, v/v). Densitometric analysis was carried out directly

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Vaidya, Shivani A., Rishikesh R. Kshirsagar, Kirti S. Laddha, and Vandana N. Jain. "ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF A NOVEL HPTLC (METHOD) FOR THE SIMULTANEOUS ESTIMATION OF BERBERINE, GALLIC ACID, QUERCETIN AND PIPERINE IN A POLYHERBAL FORMULATION." Indian Drugs 59, no. 02 (2022): 47–51. http://dx.doi.org/10.53879/id.59.02.12179.

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A novel, accurate, precise High Performance Thin Layer Chromatography (HPTLC) method for the simultaneous estimation of berberine, gallic acid, quercetin and piperine in a polyherbal formulation has been developed and validated as per ICH guidelines. A good chromatographic separation was achieved using mobile phase consisting of toluene, ethyl acetate, methanol and formic acid in the ratio of 6: 6: 2: 1 (V/V/V/V) with a wavelength of 254 nm using UV-Visible (UV-Vis) detector. The retention factors of berberine, gallic acid, quercetin and piperine were found to be 0.37, 0.51, 0.72 and 0.86, res

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Gackowski, Marcin, Marcin Koba, Katarzyna Mądra-Gackowska, and Stefan Kruszewski. "Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations." Acta Pharmaceutica 69, no. 3 (2019): 413–22. http://dx.doi.org/10.2478/acph-2019-0028.

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Abstract New methods for assaying trimetazidine dihydrochloride on the basis of thin layer chromatography and spectrophotometry are proposed and compared in the paper. In HPTLC/UV-densitometry, separation is achieved by using a mobile phase composed of ammonia-methanol (30:70, V/V) on silica gel HPTLC plates F254. Quantification using a non-linear calibration curve is accomplished by densito-metric detection at 230 nm. Derivative spectrophotometric determination of trimetazidine dihydrochloride is carried out from the fourth derivative of the absorbance at 233 nm in peak-zero mode. Statistical

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Ngo, Minh Thuy, Thi Bich Dao Dang, Dinh Chi Le, and Thi Thanh Vuong Tong. "Simultaneous determination of two UV filters (octylmethoxycinnamate, octylsalicylate) in sunscreen creams by high performance thin layer chromatography (HPTLC)." Tạp chí Nghiên cứu Dược và Thông tin Thuốc 21 (February 6, 2025): 37–46. https://doi.org/10.59882/1859-364x/264.

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Octylmethoxycinnamate (OMC), octylsalicylate (OS) are UV filters commonly used in sunscreen creams for skin protection against UV radiations. In this study, an HPTLC method was developed and validated for simultaneous determination of OMC and OS in sunscreen creams. The method was carried out using an silica gel 60 F254 thin layer plate (20 cm × 10 cm) with a mixture of cyclohexane - aceton (10:1, v/v) as mobile phase. Samples were applied on thin layer plate as 4-mm bands with applied volume at 7.0 µL per band and the distance between two consecutive bands was 9 mm. The chromatograms were dev

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