Relevant bibliographies by topics / Thin layer chromatography (TLC)

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Thin layer chromatography (TLC)'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 July 2025

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Journal articles on the topic "Thin layer chromatography (TLC)"

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Balu, Rathod* B. S. Bhosale Dr. Prachi Udapurkar Jaydip Nagane Dnyaneshwar Andhale. "Review On To Study Of Thin Layer Chromatography Of Tofacitinib Citrate." International Journal of Pharmaceutical Sciences 2, no. 7 (2024): 662–72. https://doi.org/10.5281/zenodo.12706367.

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Thin layer chromatography (TLC) was employed to study the properties and behavior of tofacitinib citrate. The aim was to develop a reliable method for the identification, separation, and analysis of this compound. The TLC technique involved using silica gel as the stationary phase and various solvent systems as the mobile phase. The results demonstrated that TLC is an effective analytical tool for monitoring the purity, identifying impurities, and assessing the stability of tofacitinib citrate. This study highlights the utility of TLC in the quality control and research of pharmaceutical compo
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Katayama, Yoshiki, and Shino Manabe. "Thin-layer chromatography（TLC）." Drug Delivery System 35, no. 2 (2020): 147–49. http://dx.doi.org/10.2745/dds.35.147.

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Verbitski, Sheryl M., Gerald T. Gourdin, Larissa M. Ikenouye, James D. McChesney, and Jana Hildreth. "Detection of Actaea racemosa Adulteration by Thin-Layer Chromatography and Combined Thin-Layer Chromatography-Bioluminescence." Journal of AOAC INTERNATIONAL 91, no. 2 (2008): 268–75. http://dx.doi.org/10.1093/jaoac/91.2.268.

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Abstract Actaea racemosa L. (black cohosh; syn. Cimicifuga racemosa L. Nutt.) is a native North American perennial whose root and rhizome preparations are commercially available as phytomedicines and dietary supplements, primarily for management of menopausal symptoms. Despite its wide use, methods that accurately identify processed A. racemosa are not well established; product adulteration remains a concern. Because of its similar appearance and growing locales, A. racemosa has been unintentionally mixed with other species of the genus, such as Actaea pachypoda Ell. (white cohosh) and more co
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Bober-Majnusz, Katarzyna, and Alina Pyka-Pająk. "Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed Using Thin-Layer Chromatography and High-Performance Thin-Layer Chromatography." Processes 12, no. 6 (2024): 1153. http://dx.doi.org/10.3390/pr12061153.

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TLC (thin-layer chromatography) and HPTLC (high-performance thin-layer chromatography) in normal (NP) and reversed (RP) phase systems were combined with densitometry to analyze caffeine, propyphenazone, and paracetamol. This work aims to check whether comparable limit of detection (LOD) values can be obtained on TLC and HPTLC plates. Analyses were performed on five (NP) or four (RP) different stationary phases (chromatographic plates), testing, in both cases, three mobile phases. It is shown that by using both TLC and HPTLC plates, it is possible to develop chromatographic conditions that enab
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Renger, Bernd. "Contemporary Thin-Layer Chromatography in Pharmaceutical Quality Control." Journal of AOAC INTERNATIONAL 81, no. 2 (1998): 333–40. http://dx.doi.org/10.1093/jaoac/81.2.333.

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abstract The progress in layers, instrumentation, and the development of automated scanning densitometers have led to a remarkable improvement of the features of thin-layer chromatography (TLC) and especially its high performance version (HPTLC). Contemporary TLC or HPTLC combines the techniques' inherent advantages which include high sample throughput, simultaneous processing of standards and samples, flexibility, easy postchro matographic visualization techniques, and the ability to handle complex or crude samples with minimum sample cleanup—with improved selectivity, sensitivity, and accura
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Sherma, Joseph. "Recent Advances in Thin-Layer Chromatography of Pesticides." Journal of AOAC INTERNATIONAL 82, no. 1 (1999): 48–54. http://dx.doi.org/10.1093/jaoac/82.1.48.

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Abstract Advances in the use of thin-layer chromatography (TLC) and high-performance TLC (HPTLC) for separation, detection, and qualitative and quantitative determination of pesticides, other agrochemicals, and related compounds are reviewed for the period 1996–1998. Analyses are covered for avariety of food, biological, and environmental samples and for residues of various pesticides, including insecticides, herbicides, and fungicides, belonging to different chemical classes. References on formulation analysis, hydrophobicity studies, and use of TLC and thin-layer radiochromatography for stud
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Mohammad, Ali, Abdul Moheman, and Gaber El-Desoky. "Amino acid and vitamin determinations by TLC/HPTLC: review of the current state." Open Chemistry 10, no. 3 (2012): 731–50. http://dx.doi.org/10.2478/s11532-012-0019-0.

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AbstractSeveral methods to determine amino acids and vitamins in biological and pharmaceutical samples have been reported. Thin layer chromatography (TLC) finds its place when the relatively costly equipment required by other methods is unavailable. This review covers the 1991–2010 literature on TLC/HPTLC (high performance thin layer chromatography) amino acid and vitamin determinations. It gives an overview of the special features as well as the problems in TLC/HPTLC determinations of amino acids and vitamins. Various chromatographic systems useful in amino acid and vitamin identification, se
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Braselton, W. Emmett, and Malgorzata Johnson. "Thin Layer Chromatography Convulsant Screen Extended by Gas Chromatography-Mass Spectrometry." Journal of Veterinary Diagnostic Investigation 15, no. 1 (2003): 42–45. http://dx.doi.org/10.1177/104063870301500109.

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Acute onset convulsive disorders in the canine may result from exposure to a variety of toxicants including strychnine, insecticides, metaldehyde, zinc phosphide, methylxanthines, drugs of abuse, bromethalin, and the tremorgenic mycotoxins (roquefortine and penitrem A). Although several of the above can be identified in a single gas chromatography-mass spectrometry (GC-MS) screen most have to be determined by separate tests. This report describes a modification of the strychnine extraction procedure, which allows thin layer chromatographic (TLC) identification of strychnine, bromethalin, roque
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Komsta, Łukasz. "Chemometrics in Fingerprinting by Means of Thin Layer Chromatography." Chromatography Research International 2012 (November 28, 2012): 1–5. http://dx.doi.org/10.1155/2012/893246.

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The paper is written as an introductory review, presenting summary of current knowledge about chemometric fingerprinting in the context of TLC, due to a rather small interest in the literature about joining TLC and chemometrics. The paper shortly covers the most important aspects of the chemometric fingerprinting in general, creating the TLC fingerprints, denoising, baseline removal, warping/registering, and chemometric processing itself. References being good candidates as a starting point are given for each topic and processing step.
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Huang, Du Shu, Yun Hui Long, Yong Hong, Jing Wang, Na Wu, and Jie Wu. "Chiral Resolution of Ofloxacin Using Carboxylated Multi-Walled Carbon Nanotubes Mediated Thin-Layer Chromatography." Materials Science Forum 809-810 (December 2014): 128–30. http://dx.doi.org/10.4028/www.scientific.net/msf.809-810.128.

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To resolute chiral ofloxacin, we demonstrated a new method of using carboxylated multi-walled carbon nanotubes (MWCNTs) impregnated thin-lay chromatography (TLC). The relative RF value of carboxylated MWCNTs impregnated TLC was 31.3. A general discussion that focuses on the possibility of functionalized MWCNTs in the field of chromatographic chiral resolution is presented.
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Dissertations / Theses on the topic "Thin layer chromatography (TLC)"

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Stephens, Frank Lanier. "Thin layer chromatography - flame ionization detection analysis of in-situ petroleum biodegradation." Texas A&M University, 2004. http://hdl.handle.net/1969.1/1039.

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This research was initiated after a 100-year flood caused an oil spill on the San Jacinto River (Houston, Texas) in October of 1994. After the floodwaters subsided the released petroleum floating on the water was deposited on the surrounding lands. The petroleum spill was used as an opportunity to research intrinsic petroleum biodegradation in a 9-acre petroleum impacted estuarine wetland. The first phase of this research (Phase I) began in December 1994, approximately 1.5 months after the spill of opportunity and involved the study and quantification of in-situ petroleum biodegradation. The
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Coelho, Susete. "Determinação expedita de aminas biogénicas em cogumelos por TLC." Master's thesis, Instituto Politécnico de Bragança, Escola Superior Agrária, 2012. http://hdl.handle.net/10198/7608.

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A presença de aminas biogénicas nos alimentos é um ponto crítico da segurança alimentar pela sua ligação a casos de intoxicações alimentares, sendo por isso importante testar e otimizar novas metodologias analíticas para a deteção e quantificação destes compostos. Neste trabalho, pretendeu-se otimizar uma metodologia baseada na técnica de cromatografia em camada fina para analisar 7 aminas biogénicas: cadaverina, espermina, espermidina, putrescina, tiramina, 2-feniletilamina e histamina. Numa primeira fase, testou-se a eficácia de separação de três eluentes distintos, tendo-se selecionando o
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Dhunna, Manan. "Desorption Electrospray Ionization Mass Spectrometry Imaging: Instrumentation, Optimization and Capabilities." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/3969.

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Desorption Electrospray Ionization Mass spectrometry Imaging (DESI-MSI) is an area of great interest and a promising tool in the field of chemical imaging. It is a powerful, label-free technique, which can determine, map and visualize different molecular compounds on a sample surface. The amount of information acquired in a single DESI-MSI experiment is enormous compared to other techniques, as it can simultaneously detect different compounds with their spatial distribution on the surface. The experiment can be used to produce two-dimensional and three-dimensional images. Chapter 2 focuses on
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Solanský, Pavel. "Využití chromatografie na tenké vrstvě k frakcionaci a charakterizaci organické hmoty izolované z alginitu." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2021. http://www.nusl.cz/ntk/nusl-445143.

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This diploma thesis is focused on the study of structure and physicochemical properties of organic fractions of humic substances, which were obtained by the method of thin-layer chromatography. Humic substances, which were used in this study, were isolated from a sample of Slovak alginite based on the procedure of the International Humic Substances Society (IHSS). The following analytical techniques were selected for the characterization of isolated humic substances: thermogravimetric and elemental analysis, molecular absorption spectroscopy (UV/Vis), Fourier transform infrared spectroscopy (F
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González, Gaarslev Natalia. "Growth and biodegradation by Sporidiobolales yeasts in vanillin-supplemented medium." Thesis, Högskolan i Gävle, Avdelningen för arbets- och folkhälsovetenskap, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-24678.

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Studies of biodegradation in lignins by basidiomycetes yeasts show the conversion of lignin in various degradation products among which vanillin, a valuable substance, suggested to be a strong inhibitor of both fermentation and growth of yeasts, stands. Sporidiobolales yeasts used in these experiments were aimed to be identified by their highly conserved ITS region as well as studied in vanillinsupplemented medium through, vanillin-supplemented plates, TLC and Neubauer’s chamber to find out which, among the several isolates tested, were the most resistant ones, understand how they take up vani
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Ma, Shuguo. "Reactions of Alcohols and Organophosphonates on Tungsten Trioxide Epitaxial Films." Fogler Library, University of Maine, 2003. http://www.library.umaine.edu/theses/pdf/MaS2003.pdf.

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Mullis, James Onis Jr. "Planar chromatography coupled with mass spectrometry." Thesis, Georgia Institute of Technology, 1992. http://hdl.handle.net/1853/27124.

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Collins, Thomas. "Characterisation of mycobacterium species using radiometric growth detection and thin-layer chromatography." Thesis, University of Ulster, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.281212.

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Yeager, Barry Todd. "New detection techniques in high performance thin layer chromatography and related studies." Diss., The University of Arizona, 1995. http://hdl.handle.net/10150/187353.

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Recently, high performance thin layer chromatography, or HPTLC has seen considerable growth as an analytical technique. Most often perceived as a semi-quantitative or preliminary technique, in reality HPTLC is an analytical technique in its own right. Modern instrumentation, improved stationary phases, and automated techniques have found their way into the realm of HPTLC, vastly improving the scope of the technique. Today, HPTLC is a very popular analytical technique, possessing many advantages such as ease of use, high throughput, high sensitivity, and low cost. HPTLC is also applicable to a
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Kanyal, Supriya Singh. "Fabrication, Characterization, Optimization and Application Development of Novel Thin-layer Chromatography Plates." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/5706.

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This dissertation describes advances in the microfabrication of thin layer chromatography (TLC) plates. These plates are prepared by the patterning of carbon nanotube (CNT) forests on substrates, followed by their infiltration with an inorganic material. This document is divided into ten sections or chapters. Chapter 1 reviews the basics of conventional TLC technology. This technology has not changed substantially in decades. This chapter also mentions some of the downsides of the conventional approach, which include unwanted interactions of the binder in the plates with the analytes, relative
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Books on the topic "Thin layer chromatography (TLC)"

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Macherey-Nagel. TLC: Thin layer chromatography. Macherey-Nagel, 1989.

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Hamilton, R. J. Thin layer chromatography. Edited by Hamilton Sheila, Kealey D, and ACOL. Published on behalf of ACOL byWiley, 1987.

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Fried, Bernard. Thin-layer chromatography. 4th ed. M. Dekker, 1999.

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Hamilton, R. J. Thin layer chromatography. Edited by Hamilton Shiela 1943-, Kealey D, and ACOL (Project). Published on behalf of ACOL, London, by Wiley, 1987.

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1934-, Jork Hellmut, ed. Thin-layer chromatography. VCH, 1994.

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Spangenberg, Bernd, Colin F. Poole, and Christel Weins. Quantitative Thin-Layer Chromatography. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-10729-0.

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1950-, Grinberg Nelu, ed. Modern thin-layer chromatography. M. Dekker, 1990.

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Geiss, F. Fundamentals of thin layer chromatography (planar chromatography). Huthig, 1987.

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Touchstone, Joseph C. Practice of thin layer chromatography. 3rd ed. Wiley, 1992.

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Joseph, Sherma, and Fried Bernard 1933-, eds. Handbook of thin-layer chromatography. 2nd ed. Marcel Dekker, 1996.

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Book chapters on the topic "Thin layer chromatography (TLC)"

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Mangold, Helmut K., Hwarald H. O. Schmid, and Egon Stahl. "Thin-Layer Chromatography (TLC)." In Methods of Biochemical Analysis. John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470110300.ch7.

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Spangenberg, Bernd, Colin F. Poole, and Christel Weins. "Fluorescence in TLC Layers." In Quantitative Thin-Layer Chromatography. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10729-0_11.

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Spangenberg, Bernd, Colin F. Poole, and Christel Weins. "Statistics for Quantitative TLC." In Quantitative Thin-Layer Chromatography. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10729-0_13.

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Spangenberg, Bernd, Colin F. Poole, and Christel Weins. "Diffuse Reflectance from TLC Layers." In Quantitative Thin-Layer Chromatography. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10729-0_10.

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Spangenberg, Bernd, Colin F. Poole, and Christel Weins. "Basis for TLC Development Techniques." In Quantitative Thin-Layer Chromatography. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10729-0_6.

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Spangenberg, Bernd, Colin F. Poole, and Christel Weins. "Planning an Analysis and Validation in TLC." In Quantitative Thin-Layer Chromatography. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10729-0_14.

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Spangenberg, Bernd, Colin F. Poole, and Christel Weins. "Theoretical Basis of Thin Layer Chromatography (TLC)." In Quantitative Thin-Layer Chromatography. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10729-0_2.

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Mohammad, A., and A. Moheman. "TLC/HPTLC in Biomedical Applications." In High-Performance Thin-Layer Chromatography (HPTLC). Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14025-9_10.

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Ranný, Mojmír. "New Perspectives in TLC on Chromarods." In Thin-Layer Chromatography with Flame Ionization Detection. Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3705-5_3.

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Henderson, R. James, and Douglas R. Tocher. "Thin-layer chromatography." In Lipid Analysis. Oxford University PressOxford, 1992. http://dx.doi.org/10.1093/oso/9780199630981.003.0003.

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Abstract Thin-layer chromatography (TLC) has long been used for the separation of lipid mixtures into their component lipid classes. Despite the fact that the more modern technique of high performance liquid chromatography (HPLC) is finding applications in lipid analysis, several features still make TLC particularly useful for this purpose. For example, although sophisticated and expensive apparatus can be purchased for TLC, good results on a qualitative basis can be obtained relatively simply using the minimum of equipment. Analyses are performed rapidly and many samples can be analysed simul
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Conference papers on the topic "Thin layer chromatography (TLC)"

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Buck, Erwin, Marvin C. Allen, Byron Sudbury, and Berit Skjellerudsveen. "Corrosion Inhibitor Detection by Thin Layer Chromatography: Development of the Technique." In CORROSION 1993. NACE International, 1993. https://doi.org/10.5006/c1993-93099.

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Abstract A recurring need with chemical corrosion inhibitors is an analytical method to quantify low inhibitor concentrations in both aqueous and hydrocarbon phases. Based on results presented in this paper, Thin Layer Chromatography (TLC) is a technique suitable to this need.
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Comizzoli, R. B., G. R. Crane, A. J. Muller, R. L. Opila, L. A. Psota-Kelty, and J. D. Sinclair. "Field Failures of Relays with Au10Ag Cc." In CORROSION 1989. NACE International, 1989. https://doi.org/10.5006/c1989-89320.

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Abstract High contact resistance has been observed in relays containing Au10Ag (10 weight percent Ag) contacts after approximately one year in the field. This alloy has been used successfully in a variety of relays for many years. Contact force measurements show that the high contact resistance was not a result of low contact force. Crazing of the polycarbonate relay cover suggested that a Freon TMC rinse of the circuit board leached material from the plastic and recondensed it in the contact region. However, gas chromatography/mass detection of solvent extracts of the metal parts from the rel
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Lytvynenko, Volodymyr, Serge Olszewski, Andrzej Smolarz, et al. "Thin-layer chromatography image segmentation for toxicological studies." In Photonics Applications in Astronomy, Communications, Industry, and High Energy Physics Experiments 2024, edited by Ryszard S. Romaniuk, Andrzej Smolarz, and Waldemar Wójcik. SPIE, 2024. https://doi.org/10.1117/12.3052160.

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Tristantini, Dewi, and Adeline Jessica. "Determination of flavonoid content of mixed herbs extract using colorimetric method and thin layer chromatography (TLC)." In SECOND INTERNATIONAL CONFERENCE OF MATHEMATICS (SICME2019). Author(s), 2019. http://dx.doi.org/10.1063/1.5096708.

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Suszyński, Z., R. Świta, and Joanna Łoś. "Thermal-wave investigation of physical and geometrical non-uniformities within thin layer chromatography (TLC) stationary phases." In 2016 Quantitative InfraRed Thermography. QIRT Council, 2016. http://dx.doi.org/10.21611/qirt.2016.129.

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Blebea, Nicoleta Mirela, and Simona Negreș. "METHODS FOR QUANTIFICATION OF THE MAIN CANNABINOIDS IN CBD OIL." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/13.

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Cannabidiol (CBD) is an alkaloid present in Cannabis sativa, together with tetrahydrocannabinol (THC) and more than 120 other substances belonging to a group of compounds named cannabinoids. Due to the continuous increased usage of CBD oils, it became necessary to be developed efficient methods for the identification of its compounds and especially for the characterization of the cannabinoids from the commercial specimens. Cannabinoids may be detected by many and different analytical methods, including immunoassays (EMIT®, Elisa, fluorescent polarization, radioimmunotest), techniques of flat c
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ARIAS, Justo Manuel, Ricardo F. LAIME, Jonathan R. OLMO, Ana G. IRIARTE, and Rosa M. S. ALVAREZ. "INNOVATIVE TECHNIQUE FOR RAPID DETECTION OF ANALYTES IN COMPLEX MATRICES." In Second Southern Science Conference - 2024. Araucária - Associação Científica, 2024. https://doi.org/10.48141/sscon_60_2024.pdf.

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In this work we present an innovative technique to detect analytes in complex matrices. Thioverbenone was synthesized using verbenone and Curphey’s reagent without further purification, and was detected in the mixture reaction using SERS spectroscopý coupled to thin layer chromatography (TLC). Combining both techniques enables the rapid and efficient detection of components in complex matrices without prior purification, thereby saving time and reagents.
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Schick, K. P., S. Shapiro, G. Tuszynski, and J. Slawek. "SULFATIDES AND GLYCOLIPIDS IN PLATELETS AND ENDOTHELIAL CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643641.

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Sulfatides are sulfated glycolipids which are negatively charged and thought to influence receptor mediated activities. Sulfatides have the capacity to provide a surface for the initiation of in vitro coagulation tests and these acidic lipids represent the potential biological surface for the initiation of the contact and intrinsic systems in vivo. Several sulfatides have been demonstrated in blood platelets. We have investigated sulfatides and other glycolipids in endothelial cells and platelets in order to define the cellular sources for sulfatides that would be available for influencing hem
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Turina, E. L., S. G. Efimenko, Yu A. Kornev та A. P. Liksutina. "Results of Сamelina oil assessment". У РАЦИОНАЛЬНОЕ ИСПОЛЬЗОВАНИЕ ПРИРОДНЫХ РЕСУРСОВ В АГРОЦЕНОЗАХ. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-15.05.2020.35.

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Camelina sativa (L.) Crantz – is an annual oilseed crop in the family Brassicaceae. The aim of the research was to study oil obtained from camelina seeds cultivated in the Crimea. Determination of fatty acid composition was carried out on the gas chromatograph “Хроматэк – Кристалл 5000” (Hromatek - Crystal 5000); automatic dosing unit ДАЖ-2М (DAJ- 2M); capillary column SolGelWax 30m × 0.25 mm × 0.5 μm; carrier gas – helium; speed – 22 centimeters per second; programming temperature –178–230 °С. The preparation of fatty acid methyl esters (FAMEs) using gas-liquid chromatography (GC) was perform
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Arslanova, G. R., D. B. Prosvirnikov, and R. G. Safin. "EXTRACTION OF PHENOLIC COMPOUNDS OF ASPEN AND WILLOW LEAVES OF THE SALICACEAE FAMILY." In Modern machines, equipment and IT solutions for industrial complex: theory and practice. Voronezh State University of Forestry and Technologies named after G.F. Morozov, Voronezh, Russia, 2021. http://dx.doi.org/10.34220/mmeitsic2021_17-22.

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The paper presents the results of studies on the extraction of phenolic compounds from the leaves of trees of the Salicaceae family, namely aspen and willow. A method for extracting phenolic compounds of salicin and quercetin by extraction using ethyl alcohol as a solvent is described. The method of identification of salicin and quercetin by thin-layer chromatography (TLC) is described. Digital processing of the obtained results was carried out using the computer program “Sorbfil Visualizer” (Krasnodar). The optimal operating parameters of the process, allowing to obtain the highest yield of t
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Reports on the topic "Thin layer chromatography (TLC)"

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เพียภูเขียว, จิตรตรา. การย่อยสลายทางชีวภาพของเอ็นโดซัลแฟนโดยรา : รายงานวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2008. https://doi.org/10.58837/chula.res.2008.49.

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เอ็นโดซัลแฟน เป็นสารกำจัดแมลงศัตรูพืชในกลุ่มคลอรีนอินทรีย์ที่มีความเป็นพิษ และมีความคงทนสูงในสิ่งแวดล้อม เมื่อเกิดการปนเปื้อนหรือแพร่กระจาย ย่อมก่อให้เกิดภัยต่อมนุษย์ รวมถึงสิ่งมีชีวิตอื่นๆ ในระบบนิเวศ งานวิจัยนี้ จึงเป็นการศึกษาถึงการคัดแยกราในธรรมชาติที่มีความสามารถในการย่อยสลายเอ็นโดซัลแฟนให้อยู่ในรูปที่มีความเป็นพิษน้อยลง โดยราที่คัดแยกมาศึกษา ได้แก่ ราในดิน ราและเห็ดที่ขึ้นบนซากพืช รวมทั้งสิ้น 57 ไอโซเลต ซึ่งผลการทดสอบความสามารถในการย่อยสลายขั้นปฐมภูมิด้วยวิธี Thin Layer Chromatography (TLC) พบว่า ภายหลังจากการบ่มเชื้อด้วยอาหารเลี้ยงเชื้อชนิด Czapek’s Dox Medium เป็นเวลา 20 วัน ราไอโซเลต
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2

Chutimaworapan, Suchada, Chaiyo Chaichantippayuth, and Areerat Laopaksa. Formulation of pharmaceutical products of Garcinia mangostana Linn. extracts. Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.32.

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Part I: The purpose of the investigation was to develop the extraction process that was simple, practical and giving high yield. The maceration of dried powder of Garcinia mangostana fruit husk with ethyl acetate gave yellow crystalline powder of mangostin. The yield was calculated as 7.47%. The identification of the Garcinia mangostanahusk extract was carried out by thin-layer chromatography (TLC) and differential scanning calorimetry. The TLC of mangostin was done by using the alumina sheet and ethyl acetate: hexane (3:1) as mobile phase. The Rf value as compared with standard mangostin was
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3

Yinfa, Ma. Indirect fluorometric detection techniques on thin layer chromatography and effect of ultrasound on gel electrophoresis. Office of Scientific and Technical Information (OSTI), 1990. http://dx.doi.org/10.2172/6045672.

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4

Zhu, Jianzhong. New development of laser-based techniques in applications of thin-layer chromatography, microprobe elemental analysis and gas phase pyrolysis. Office of Scientific and Technical Information (OSTI), 1990. http://dx.doi.org/10.2172/7121607.

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5

Whipple, R. Field Analysis of Propellant Stabilizers and their Daughter Products in any Propellant Formulation by Thin-Layer Chromatography Year End Report 2003. Office of Scientific and Technical Information (OSTI), 2003. http://dx.doi.org/10.2172/15009754.

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6

พงษ์สามารถ, สุนันท์, та มณีวรรณ สุขสมทิพย์. การศึกษาผลต่อการดูดซึมสารอาหารไขมันของสารโพลีแซคคาไรด์จากเปลือกผลทุเรียนในหลอดทดลอง. จุฬาลงกรณ์มหาวิทยาลัย, 2003. https://doi.org/10.58837/chula.res.2003.31.

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ศึกษาคุณสมบัติทางชีวภาพของเจลโพลีแซคคาไรด์ (PG) สกัดจากเปลือกของผลทุเรียน (Durio zibethinus L.) เพื่อประเมินคุณสมบัติการทนต่อการย่อยด้วยเอนไซม์อัลฟาอมัยเลสและคุณสมบัติการเก็บกักเก็บสารลิปิด โดยทำการศึกษาในหลอดทดลอง พบว่า ผงเจลโพลีแซคคาไรด์พองตัวในน้ำจับเป็นชั้นข้นหนืด 2% ของสารละลาย PG มีค่าความหนืดเท่ากับ 279.36 +-25.87 cps. PG ทนต่อการถูกย่อยด้วยเอนไซม์อัลฟาอมัยเลสซึ่งถูกย่อยได้เพียงส่วนน้อยโดยที่โครงสร้างอัลฟาฮิลิคของ PG หายไปจากการตรวจสอบด้วยน้ำยาไอโอดีน หลังการย่อยด้วยเอนไซม์อัลฟาอมัยเลส พบปริมาณน้ำตาลรีดิวส์น้อยมาก ไม่พบน้ำตาล monosaccharides จากการตรวจสอบด้วย O-toluidine test เทียบกับ s
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7

ธนาศุภวัฒน์, สมบูรณ์, กิตติศักดิ์ ลิขิตวิทยาวุฒิ та นิจศิริ เรืองรังษี. การศึกษาเปรียบเทียบการผลิตสารปฏิชีวนะ โดยเชื้อMicromonospora และเชื้อสกุลอื่นที่เกี่ยวข้อง. จุฬาลงกรณ์มหาวิทยาลัย, 1989. https://doi.org/10.58837/chula.res.1989.8.

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ได้ศึกษาคัดเลือกและตรวจสอบเอกลักษณ์เชื้อ actinomycetes ที่สามารถผลิตสารปฏิชีวนะ เชื้อที่แยกได้ส่วนหใญ่สามารถผลิตสารที่ยับยั้งการเจริญของเชื้อ Staphylococcus aureus ATCC 25923 และ Bacillus aubtilis ATCC 6633, และมีบางไอโซเลต สามารถยับยั้งการเจริญของเชื้อ Mycobacterium smegmatis ATCC 607 และ Candida albicans IFO 0583. เชื้อทุกไอโซเลตไม่แสดงฤทธิ์การยับยั้ง Pseudomonas aeruginosa NCTC 10490, Aeromonas hydrophila JCM 2359, Aeromonas sobria JCM 2365, Serratia marcescens IAM 73060 และ Escherichia coli NIHJ JC-2 พบว่าเชื้อทั้งหมด 47 ไอโซเลตเป็น Micromonospora sp. 20 ไอโซเลต, Streptomyces sp. 15 ไอโซเล
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8

ธนียวัน, จิราภรณ์, ไพเราะ ปิ่นพานิชการ, สุเพท ธนียวัน, สุชาดา จันทร์ประทีป та วรรณา ตุลยธัญ. การผลิตสารลดแรงตึงผิวชีวภาพจากจุลินทรีย์เพื่อใช้ในอุตสาหกรรมอาหาร : รายงานการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2009. https://doi.org/10.58837/chula.res.2009.35.

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ยีสต์ Pichia anomala PY1 ที่ใช้ในการทดลองนี้ถูกแยกมาจากข้าวหมากที่อำเภอ พนัสนิคม จังหวัดชลบุรี จากการศึกษาการเจริญ และการผลิตสารลดแรงตึงผิวชีวภาพในอาหารเหลว กำหนดสูตรที่มีน้ำมันถั่วเหลือง 4% เป็นแหล่งคาร์บอน มี NaNO₃ 0.4% เป็นแหล่งไนโตรเจนควบคุมค่าความเป็นกรดด่างเริ่มต้นเท่ากับ 5.5 บ่มที่อุณหภูมิ 30 องศาเซลเซียส เขย่าอัตราเร็ว 200 รอบต่อนาที เป็นเวลา 7 วัน โดยส่วนน้ำเลี้ยงเชื้อที่ไม่มีเซลล์ มีค่าการกระจายน้ำมันสูงสุด 5.07 ตารางเซนติเมตร มีค่าแรงตึงผิวต่ำสุดในวันที่ 7 เท่ากับ 38 มิลลินิวตันต่อเมตร และเมื่อทำการก่อการกลายพันธ์เพื่อเพิ่มการผลิตสารลดแรงตึงผิวชีวภาพโดยสาร Ethylmethane sulfonate คัด
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