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Journal articles on the topic 'Time-lapse video analysis'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

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1

Watanabe, S., M. Kamihata, R. Matsunaga, A. Kuwahata, M. Ochi, and T. Horiuchi. "Effect of an abnormal first cleavage on embryonic development: time-lapse video analysis." Fertility and Sterility 100, no. 3 (September 2013): S245—S246. http://dx.doi.org/10.1016/j.fertnstert.2013.07.1166.

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Gerlach, J., J. Vienken, P. Walker, and K. Affeld. "Computer Aided Time-Lapse Video Analysis of Hepatocyte Morphology during Adhesion to Cellulose Membranes." International Journal of Artificial Organs 13, no. 6 (June 1990): 365–69. http://dx.doi.org/10.1177/039139889001300607.

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3

Feyeux, M., A. Reignier, M. Mocaer, J. Lammers, D. Meistermann, P. Barrière, P. Paul-Gilloteaux, L. David, and T. Fréour. "Development of automated annotation software for human embryo morphokinetics." Human Reproduction 35, no. 3 (March 2020): 557–64. http://dx.doi.org/10.1093/humrep/deaa001.

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Abstract STUDY QUESTION Is it possible to develop an automated annotation tool for human embryo development in time-lapse devices based on image analysis? SUMMARY ANSWER We developed and validated an automated software for the annotation of human embryo morphokinetic parameters, having a good concordance with expert manual annotation on 701 time-lapse videos. WHAT IS KNOWN ALREADY Morphokinetic parameters obtained with time-lapse devices are increasingly used for the assessment of human embryo quality. However, their annotation is time-consuming and can be slightly operator-dependent, highligh

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Hoepfner, Dominic, Arndt Brachat, and Peter Philippsen. "Time-Lapse Video Microscopy Analysis Reveals Astral Microtubule Detachment in the Yeast Spindle Pole Mutantcnm67." Molecular Biology of the Cell 11, no. 4 (April 2000): 1197–211. http://dx.doi.org/10.1091/mbc.11.4.1197.

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Saccharomyces cerevisiae cnm67Δ cells lack the spindle pole body (SPB) outer plaque, the main attachment site for astral (cytoplasmic) microtubules, leading to frequent nuclear segregation failure. We monitored dynamics of green fluorescent protein–labeled nuclei and microtubules over several cell cycles. Early nuclear migration steps such as nuclear positioning and spindle orientation were slightly affected, but late phases such as rapid oscillations and insertion of the anaphase nucleus into the bud neck were mostly absent. Analyzes of microtubule dynamics revealed normal behavior of the nuc

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5

Hagmann, Jörg, Daniel Dagan, and Max M. Burger. "Release of endosomal content induced by plasma membrane tension: Video image intensification time lapse analysis." Experimental Cell Research 198, no. 2 (February 1992): 298–304. http://dx.doi.org/10.1016/0014-4827(92)90383-j.

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6

Dover, R., and C. S. Potten. "Heterogeneity and cell cycle analyses from time-lapse studies of human keratinocytes in vitro." Journal of Cell Science 89, no. 3 (March 1, 1988): 359–64. http://dx.doi.org/10.1242/jcs.89.3.359.

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We have analysed the behaviour of cultured epidermal keratinocytes using time-lapse video recordings. We have found evidence for heterogeneity in the behaviour of the cells. Some lines underwent extensive self-renewal, thus expanding the population, while others produced daughter cells that migrated suprabasally and are presumed to have undergone terminal differentiation. We also present kinetic data on the cell cycle times, mitotic durations and post-mitotic residence times. The latter is the time between a cell's birth and eventual suprabasal migration. The data suggest that the ‘decision’ t

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Bertani, N. "Neurogenic potential of human mesenchymal stem cells revisited: analysis by immunostaining, time-lapse video and microarray." Journal of Cell Science 118, no. 17 (September 1, 2005): 3925–36. http://dx.doi.org/10.1242/jcs.02511.

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8

Skilton, Rachel J., Lesley T. Cutcliffe, David Barlow, Yibing Wang, Omar Salim, Paul R. Lambden, and Ian N. Clarke. "Penicillin Induced Persistence in Chlamydia trachomatis: High Quality Time Lapse Video Analysis of the Developmental Cycle." PLoS ONE 4, no. 11 (November 6, 2009): e7723. http://dx.doi.org/10.1371/journal.pone.0007723.

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9

Nagy, Gabor, Grant W. Hennig, Katalin Petrenyi, Laszlo Kovacs, Istvan Pocsi, Viktor Dombradi, and Gaspar Banfalvi. "Time-lapse video microscopy and image analysis of adherence and growth patterns of Candida albicans strains." Applied Microbiology and Biotechnology 98, no. 11 (April 2, 2014): 5185–94. http://dx.doi.org/10.1007/s00253-014-5696-5.

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Siegert, F., C. J. Weijer, A. Nomura, and H. Miike. "A gradient method for the quantitative analysis of cell movement and tissue flow and its application to the analysis of multicellular Dictyostelium development." Journal of Cell Science 107, no. 1 (January 1, 1994): 97–104. http://dx.doi.org/10.1242/jcs.107.1.97.

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We describe the application of a novel image processing method, which allows quantitative analysis of cell and tissue movement in a series of digitized video images. The result is a vector velocity field showing average direction and velocity of movement for every pixel in the frame. We apply this method to the analysis of cell movement during different stages of the Dictyostelium developmental cycle. We analysed time-lapse video recordings of cell movement in single cells, mounds and slugs. The program can correctly assess the speed and direction of movement of either unlabelled or labelled c

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11

Meyers, S., V. Burruel, M. Kato, A. de la Fuente, D. Orellana, C. Renaudin, and G. Dujovne. "Equine non-invasive time-lapse imaging and blastocyst development." Reproduction, Fertility and Development 31, no. 12 (2019): 1874. http://dx.doi.org/10.1071/rd19260.

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In this study we examined the timeline of mitotic events of invitro-produced equine embryos that progressed to blastocyst stage using non-invasive time-lapse microscopy (TLM). Intracytoplasmic sperm injection (ICSI) embryos were cultured using a self-contained imaging incubator system (Miri®TL; Esco Technologies) that captured brightfield images at 5-min intervals that were then generated into video for retrospective analysis. For all embryos that progressed to the blastocyst stage, the initial event of extrusion of acellular debris preceded all first cleavages and occurred at mean (±s.e.m.) t

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12

Grönniger, Elke, Sonja Wessel, Sonja Christin Kühn, Jörn Söhle, Horst Wenck, Franz Stäb, and Marc Winnefeld. "A new protocol for functional analysis of adipogenesis using reverse transfection technology and time-lapse video microscopy." Cell Biology International 34, no. 7 (May 24, 2010): 737–46. http://dx.doi.org/10.1042/cbi20090299.

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13

Magyar-Lehmann, Stefanie, Caterina Savi Suter, Werner Stahel, and Melitta Schachner. "Behaviour of Small Inhibitory Interneurons in Early Postnatal Mouse Cerebellar Microexplant Cultures: A Video Time-lapse Analysis." European Journal of Neuroscience 7, no. 7 (July 1995): 1449–59. http://dx.doi.org/10.1111/j.1460-9568.1995.tb01140.x.

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14

Mineyuki, Yoshinobu, and Brian E. S. Gunning. "Streak time-lapse video microscopy: analysis of protoplasmic motility and cell division in Tradescantia stamen hair cells." Journal of Microscopy 150, no. 1 (April 1988): 41–55. http://dx.doi.org/10.1111/j.1365-2818.1988.tb04585.x.

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15

Straight, Aaron F., John W. Sedat, and Andrew W. Murray. "Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast." Journal of Cell Biology 143, no. 3 (November 2, 1998): 687–94. http://dx.doi.org/10.1083/jcb.143.3.687.

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The mitotic spindle is a complex and dynamic structure. Genetic analysis in budding yeast has identified two sets of kinesin-like motors, Cin8p and Kip1p, and Kar3p and Kip3p, that have overlapping functions in mitosis. We have studied the role of three of these motors by video microscopy of motor mutants whose microtubules and centromeres were marked with green fluorescent protein. Despite their functional overlap, each motor mutant has a specific defect in mitosis: cin8Δ mutants lack the rapid phase of anaphase B, kip1Δ mutants show defects in the slow phase of anaphase B, and kip3Δ mutants

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16

Aftab, Obaid, Mårten Fryknäs, Ulf Hammerling, Rolf Larsson, and Mats G. Gustafsson. "Detection of Cell Aggregation and Altered Cell Viability by Automated Label-Free Video Microscopy." Journal of Biomolecular Screening 20, no. 3 (December 17, 2014): 372–81. http://dx.doi.org/10.1177/1087057114562158.

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Automated phase-contrast video microscopy now makes it feasible to monitor a high-throughput (HT) screening experiment in a 384-well microtiter plate format by collecting one time-lapse video per well. Being a very cost-effective and label-free monitoring method, its potential as an alternative to cell viability assays was evaluated. Three simple morphology feature extraction and comparison algorithms were developed and implemented for analysis of differentially time-evolving morphologies (DTEMs) monitored in phase-contrast microscopy videos. The most promising layout, pixel histogram hierarch

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17

Lin, Sabrina Chia-Chin, Jo-Hao Weng, Kimberly Lung, Jonathan Balakumar, Victor Slupski, and Prue Talbot. "Analysis of Human Embryonic Stem Cell Behavior in Control and Experimental Conditions Using Time-Lapse Video Microscopy Endpoints." Biology of Reproduction 81, Suppl_1 (July 1, 2009): 667. http://dx.doi.org/10.1093/biolreprod/81.s1.667.

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18

Guo, Jun, Xuefei Yan, Shiyang Li, Johanna Van der Walt, Guangzhao Guan, and Li Mei. "Quantitative and qualitative analyses of orthodontic-related videos on YouTube." Angle Orthodontist 90, no. 3 (February 3, 2020): 411–18. http://dx.doi.org/10.2319/082019-542.1.

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ABSTRACT Objectives To investigate content of orthodontic-related videos on YouTube to improve the understanding of orthodontic patients' perceptions and treatment experiences. Materials and Methods A systematic search was conducted on YouTube on March 20, 2018, and updated on August 4, 2019, to identify all relevant videos using search terms “orthodontic,” “orthodontics,” “braces,” and “orthodontic braces.” The data set was captured from YouTube Data API (Application Programming Interface) and stored in an Excel database using a query function written in Python. All videos captured were viewe

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19

Gwydir, Stacy, Christos Kirgios, Helen Buettner, and Stanley Dunn. "Analysis of time-varying imaging from phase-contrast microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 50, no. 2 (August 1992): 1004–5. http://dx.doi.org/10.1017/s0424820100129656.

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Quantitative analysis of neurite outgrowth and growth cone (filopodia and lamellipodia) motility provides key information for developing new therapeutic strategies for treating peripheral nerve injury and disease. Neurite outgrowth and growth cone properties will be measured for neurons taken from the dorsal root ganglia of 8-day old chick embryos. Ganglia will be enzymatically dissociated into single neurons and cultured in a serum-free medium. Cultures will be grown on glass coverslips coated with the basement membrane protein laminin. Following a brief incubation period of 4 hr to allow the

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Forrester, Helen B., Norman Albright, C. Clifton Ling, and William C. Dewey. "Computerized Video Time-Lapse Analysis of Apoptosis of REC:Myc Cells X-Irradiated in Different Phases of the Cell Cycle." Radiation Research 154, no. 6 (December 2000): 625–39. http://dx.doi.org/10.1667/0033-7587(2000)154[0625:cvtlao]2.0.co;2.

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Rezaie, P., G. Trillo-Pazos, J. Greenwood, I. P. Everall, and D. K. Male. "Motility and Ramification of Human Fetal Microglia in Culture: An Investigation Using Time-Lapse Video Microscopy and Image Analysis." Experimental Cell Research 274, no. 1 (March 2002): 68–82. http://dx.doi.org/10.1006/excr.2001.5431.

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22

Watanabe, S., M. Kamihata, R. Matsunaga, A. Kuwahata, M. Ochi, and T. Horiuchi. "Contractions during the expanded blastocyst stage decrease the success rate of frozen-thawed blastocyst transfer: time-lapse video analysis." Fertility and Sterility 100, no. 3 (September 2013): S245. http://dx.doi.org/10.1016/j.fertnstert.2013.07.1165.

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23

Schiraldi, Chiara, Silvia Zappavigna, Antonella D' Agostino, Stefania Porto, Ornella Gaito, Sara Lusa, Monica Lamberti, Mario De Rosa, Giuseppe De Rosa, and Michele Caraglia. "Nanoparticles for the delivery of zoledronic acid to prostate cancer cells: A comparative analysis through time lapse video-microscopy technique." Cancer Biology & Therapy 15, no. 11 (November 2, 2014): 1524–32. http://dx.doi.org/10.4161/15384047.2014.955989.

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Huth, Johannes, Malte Buchholz, Johann M. Kraus, Martin Schmucker, Götz von Wichert, Denis Krndija, Thomas Seufferlein, Thomas M. Gress, and Hans A. Kestler. "Significantly improved precision of cell migration analysis in time-lapse video microscopy through use of a fully automated tracking system." BMC Cell Biology 11, no. 1 (2010): 24. http://dx.doi.org/10.1186/1471-2121-11-24.

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25

Oertel, Anke, Nicole Aichinger, Romana Hochreiter, Josef Thalhamer, and Ursula Lütz-Meindl. "ANALYSIS OF MUCILAGE SECRETION AND EXCRETION IN MICRASTERIAS (CHLOROPHYTA) BY MEANS OF IMMUNOELECTRON MICROSCOPY AND DIGITAL TIME LAPSE VIDEO MICROSCOPY1." Journal of Phycology 40, no. 4 (July 9, 2004): 711–20. http://dx.doi.org/10.1111/j.1529-8817.2004.03222.x.

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26

Červinka, Miroslav. "Time-lapse Phase-contrast Microphotography of Cell Populations as a Basis for Improvement of In Vitro Toxicity Assessment." Alternatives to Laboratory Animals 20, no. 2 (April 1992): 302–6. http://dx.doi.org/10.1177/026119299202000223.

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Recent trends in the field of in vitro toxicology have centred around the validation of in vitro methods. The ultimate goal is to obtain pertinent data with the minimum of effort. In our laboratory, we have used toxicological methods based on the evaluation of cell morphology and cell proliferation. A method suitable for this purpose is time-lapse microcinematographic (or video) recording of cellular changes, which we used for many years. For practical in vitro toxicity testing, however, this method is far too complicated. Therefore, we have tried to develop a simple modification for the evalu

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Lu, Hengyang, Jiabing Li, Melisa A. Martinez-Paniagua, Irfan N. Bandey, Amit Amritkar, Harjeet Singh, David Mayerich, Navin Varadarajan, and Badrinath Roysam. "TIMING 2.0: high-throughput single-cell profiling of dynamic cell–cell interactions by time-lapse imaging microscopy in nanowell grids." Bioinformatics 35, no. 4 (August 1, 2018): 706–8. http://dx.doi.org/10.1093/bioinformatics/bty676.

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Abstract Motivation Automated profiling of cell–cell interactions from high-throughput time-lapse imaging microscopy data of cells in nanowell grids (TIMING) has led to fundamental insights into cell–cell interactions in immunotherapy. This application note aims to enable widespread adoption of TIMING by (i) enabling the computations to occur on a desktop computer with a graphical processing unit instead of a server; (ii) enabling image acquisition and analysis to occur in the laboratory avoiding network data transfers to/from a server and (iii) providing a comprehensive graphical user interfa

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Prieur-Carrillo, Geraldine, Kenneth Chu, Johan Lindqvist, and William C. Dewey. "Computerized Video Time-Lapse (CVTL) Analysis of the Fate of Giant Cells Produced by X-Irradiating EJ30 Human Bladder Carcinoma Cells." Radiation Research 159, no. 6 (June 2003): 705–12. http://dx.doi.org/10.1667/rr3009.

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29

Tanaka, Satoshi, Yuko Sekino, and Tomoaki Shirao. "1105 Inhibition of the speed of of cerebellar granule cell migration in vitro by NT-3-A time-lapse video microscopic analysis." Neuroscience Research 28 (January 1997): S136. http://dx.doi.org/10.1016/s0168-0102(97)90362-x.

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Sretavan, David W., and Louis F. Reichardt. "Time-lapse video analysis of retinal ganglion cell axon pathfinding at the mammalian optic chiasm: Growth cone guidance using intrinsic chiasm cues." Neuron 10, no. 4 (April 1993): 761–77. http://dx.doi.org/10.1016/0896-6273(93)90176-r.

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31

Palevitz, Barry A. "Division plane determination in guard mother cells of Allium: Video time-lapse analysis of nuclear movements and phragmoplast rotation in the cortex." Developmental Biology 117, no. 2 (October 1986): 644–54. http://dx.doi.org/10.1016/0012-1606(86)90333-7.

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Swift, Lucy, Chunfen Zhang, Ravi Shah, Tanya Trippett, and Aru Narendran. "In Vitro Activity and Target Modulation of PV-10 Against Relapsed and Refractory Pediatric Leukemia." Blood 132, Supplement 1 (November 29, 2018): 5207. http://dx.doi.org/10.1182/blood-2018-99-119438.

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Abstract Introduction: Leukemias are the most common childhood cancers, accounting for 30% of all pediatric cancer diagnoses. Although the survival rate for pediatric leukemia has greatly improved, relapse is a major cause of treatment failure. Approximately 15-20% of pediatric acute lymphoblastic leukemia (ALL) patients and 30-40% of acute myeloid leukemia (AML) patients relapse, with relapsed ALL identified as the fourth most common malignancy in children. Treatment of relapsed pediatric leukemia includes intensification of chemotherapeutic regimens and use of bone marrow transplantation (BM

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Grundler, F., L. Schnibbe, and U. Wyss. "In vitro studies on the behaviour of second-stage juveniles of Heterodera schachtii (Nematoda: Heteroderidae) in response to host plant root exudates." Parasitology 103, no. 1 (August 1991): 149–55. http://dx.doi.org/10.1017/s0031182000059394.

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The behaviour of Heterodera schachtii second-stage juveniles in response to mustard (Sinapis alba) rooxudates was observed and analysed under aseptic conditions in a standardized bioassay. Aggregation of juveniles on an agarose layer occurred within less than 30 min in the area where root exudates had been applied and persisted for several hours. Analysis of time-lapse video recordings showed that the aggregation did not result from a directed orientation of the juvenile towards the root exudate. This was supported by an orientation assay using single juveniles. Aggregated juveniles showed pre

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Bohrmann, J., and K. Biber. "Cytoskeleton-dependent transport of cytoplasmic particles in previtellogenic to mid-vitellogenic ovarian follicles of Drosophila: time-lapse analysis using video-enhanced contrast microscopy." Journal of Cell Science 107, no. 4 (April 1, 1994): 849–58. http://dx.doi.org/10.1242/jcs.107.4.849.

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In Drosophila oogenesis, several morphogenetic determinants and other developmental factors synthesized in the nurse cells have been shown to accumulate in the oocyte during pre- to mid-vitellogenic stages. However, the mechanisms of the involved intercellular transport processes that seem to be rather selective have not been revealed so far. We have investigated in vitro, by means of video-enhanced contrast time-lapse microscopy, the transport of cytoplasmic particles from the nurse cells through ring canals into the oocyte during oogenesis stages 6–10A. At stage 7, we first observed single p

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Ward, Mandy J., Kenny C. Mok, and David R. Zusman. "Myxococcus xanthus Displays Frz-Dependent Chemokinetic Behavior during Vegetative Swarming." Journal of Bacteriology 180, no. 2 (January 15, 1998): 440–43. http://dx.doi.org/10.1128/jb.180.2.440-443.1998.

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ABSTRACT Myxococcus xanthus has been shown to utilize both directed (tactic) and undirected (kinetic) movements during different stages of its complex life cycle. We have used time-lapse video microscopic analysis to separate tactic and kinetic behaviors associated specifically with vegetatively swarming cells. Isolated individual cells separated by a thin agar barrier from mature swarms showed significant increases in gliding velocity compared to that of similar cells some distance from the swarm. This orthokinetic behavior was independent of the frequency of reversals of gliding direction (k

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CROMARTY, S. I., J. S. COBB, and G. KASS-SIMON. "Behavioral Analysis of the Escape Response in the Juvenile Lobster Homarus Americanus Over the Molt Cycle." Journal of Experimental Biology 158, no. 1 (July 1, 1991): 565–81. http://dx.doi.org/10.1242/jeb.158.1.565.

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1. Components of the escape response of the American lobster were compared over the molt cycle. Number of tailflips, frequency, duration and distance were measured. Velocity, acceleration, force and work were computed from the above measurements, using time-lapse video-recordings of escaping lobsters. 2. Soft-shelled postmolt lobsters (stage B) traveled further, spent more time tailflipping and performed a larger number of tailflips than hard-shelled premolt lobsters (stage D). Hard-shelled lobsters had a more forceful initial power swim, achieved a higher overall velocity and acceleration and

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Kil, Nuray, Katrin Ertelt, and Ulrike Auer. "Development and Validation of an Automated Video Tracking Model for Stabled Horses." Animals 10, no. 12 (November 30, 2020): 2258. http://dx.doi.org/10.3390/ani10122258.

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Changes in behaviour are often caused by painful conditions. Therefore, the assessment of behaviour is important for the recognition of pain, but also for the assessment of quality of life. Automated detection of movement and the behaviour of a horse in the box stall should represent a significant advancement. In this study, videos of horses in an animal hospital were recorded using an action camera and a time-lapse mode. These videos were processed using the convolutional neural network Loopy for automated prediction of body parts. Development of the model was carried out in several steps, in

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Saisho, Yoshifumi, Erica Manesso, Tatyana Gurlo, Chang-jiang Huang, Gianna M. Toffolo, Claudio Cobelli та Peter C. Butler. "Development of factors to convert frequency to rate for β-cell replication and apoptosis quantified by time-lapse video microscopy and immunohistochemistry". American Journal of Physiology-Endocrinology and Metabolism 296, № 1 (січень 2009): E89—E96. http://dx.doi.org/10.1152/ajpendo.90697.2008.

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An obstacle to development of methods to quantify β-cell turnover from pancreas tissue is the lack of conversion factors for the frequency of β-cell replication or apoptosis detected by immunohistochemistry to rates of replication or apoptosis. We addressed this obstacle in islets from 1-mo-old rats by quantifying the relationship between the rate of β-cell replication observed directly by time-lapse video microscopy (TLVM) and the frequency of β-cell replication in the same islets detected by immunohistochemistry using antibodies against Ki67 and insulin in the same islets fixed immediately a

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Glassmann, Alexander, Carmen Carrillo Garcia, Viktor Janzen, Dominik Kraus, Nadine Veit, Jochen Winter, and Rainer Probstmeier. "Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells." Analytical Cellular Pathology 2018 (October 10, 2018): 1–7. http://dx.doi.org/10.1155/2018/1754085.

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Cultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or a lack of proliferation in a concentration-dependent manner. This inhibition of proliferation is accompanied by the generation of polyploid giant cancer cells (PGCCs) that are characterized by cell flattening, increased cell size, polyploidy, and polynucleation as determined by crystal violet staining, BrdU and DiI labelling, and flow cytometry as well as video time-lapse analysis. Continuous SSP treatment of A549 cells can preserve PGCCs for at least two months in a r

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Chu, Kenneth, Edith A. Leonhardt, Maxine Trinh, Geraldine Prieur-Carrillo, Johan Lindqvist, Norman Albright, C. Clifton Ling, and William C. Dewey. "Computerized Video Time-Lapse (CVTL) Analysis of Cell Death Kinetics in Human Bladder Carcinoma Cells (EJ30) X-Irradiated in Different Phases of the Cell Cycle." Radiation Research 158, no. 6 (December 2002): 667–77. http://dx.doi.org/10.1667/0033-7587(2002)158[0667:cvtlca]2.0.co;2.

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Salmon, K., D. Johnson, C. Underberger, D. L. Hill, M. Surrey, H. Danzer, S. Ghadir, W. Chang, C. Alexander, and J. Barritt. "Time-lapse video analysis demonstrates laser-assisted hatching of ∼33% of the zona-pellucida after blastocyst warming significantly improves an embryo’s ability to fully hatch." Fertility and Sterility 104, no. 3 (September 2015): e188. http://dx.doi.org/10.1016/j.fertnstert.2015.07.583.

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Heinzen, Robert A., Scott S. Grieshaber, Levi S. Van Kirk, and Clinton J. Devin. "Dynamics of Actin-Based Movement byRickettsia rickettsii in Vero Cells." Infection and Immunity 67, no. 8 (August 1, 1999): 4201–7. http://dx.doi.org/10.1128/iai.67.8.4201-4207.1999.

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ABSTRACT Actin-based motility (ABM) is a virulence mechanism exploited by invasive bacterial pathogens in the genera Listeria,Shigella, and Rickettsia. Due to experimental constraints imposed by the lack of genetic tools and their obligate intracellular nature, little is known about rickettsial ABM relative toListeria and Shigella ABM systems. In this study, we directly compared the dynamics and behavior of ABM ofRickettsia rickettsii and Listeria monocytogenes. A time-lapse video of moving intracellular bacteria was obtained by laser-scanning confocal microscopy of infected Vero cells synthes

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Zahm, Jean-Marie, Sonia Baconnais, Serge Monier, Noël Bonnet, Ginette Bessède, Philippe Gambert, Edith Puchelle, and Gérard Lizard. "Chronology of cellular alterations during 7-ketocholesterol-induced cell death on A7R5 rat smooth muscle cells: Analysis by time lapse-video microscopy and conventional fluorescence microscopy." Cytometry Part A 52A, no. 2 (March 19, 2003): 57–69. http://dx.doi.org/10.1002/cyto.a.10027.

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Swift, Lucy, Chunfen Zhang, Antony Pfaffle, Paul Chew, Olga Kovalchuk, Tanya Maria Trippett, and Aru Narendran. "Effective targeted antitumor activity of the antimicrobial agent taurolidine against relapsed/refractory neuroblastoma: Cytotoxicity, target modulation and tumor xenograft studies." Journal of Clinical Oncology 37, no. 15_suppl (May 20, 2019): e21502-e21502. http://dx.doi.org/10.1200/jco.2019.37.15_suppl.e21502.

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e21502 Background: Neuroblastoma (NB) is the most common extracranial solid tumor and one of the most complex and difficult to treat diseases in pediatrics. Currently, even with highly aggressive treatment protocols, the prognosis for patients with high-risk and relapsed NB remains poor. Hence, there is a clear need to identify new agents and novel therapeutic strategies for the treatment of these children. Taurolidine (TRD) is derived from the aminosulfoacid taurine and has known anti-microbial and anti-inflammatory properties. TRD has demonstrated anti-neoplastic activity against a range of

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Breen, E. J., P. H. Vardy, and K. L. Williams. "Movement of the multicellular slug stage of Dictyostelium discoideum: an analytical approach." Development 101, no. 2 (October 1, 1987): 313–21. http://dx.doi.org/10.1242/dev.101.2.313.

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Time-lapse video recordings of migrating multicellular slugs of Dictyostelium discoideum were subjected to image analysis. A transient ‘collar-like’ structure was identified at the anterior end of the slug. This collar remains stationary in the wild- type strain WS380B; it is observed shortly after the advancing tip contacts the substratum. Stationary collars formed approximately every 12min; they were matched with patterns revealed on the underside of slime trails with FITC-coupled monoclonal antibody MUD50. It is proposed that stationary collars are involved with the forward movement of the

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Blue, Ben, Elena Vayndorf, Matt Kaeberlein, and Jason Pitt. "A ROBOTIC SYSTEM FOR HIGH-THROUGHPUT AUTOMATED LIFESPAN ANALYSIS IN C. ELEGANS." Innovation in Aging 3, Supplement_1 (November 2019): S102. http://dx.doi.org/10.1093/geroni/igz038.383.

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Abstract Over the past decade, the identification of potential genetic and pharmacological modifiers of lifespan and age-related pathologies in C. elegans and other model organisms has yielded fruitful leads for follow-up investigation. A major limitation of such studies, however, is that they are often time-consuming and labor-intensive. The advent of affordable high-quality digital cameras, robotics systems, and 3D printers, as well as the decreasing costs of image storage and processing have allowed us to automate data capture and analysis at an unprecedented scale. To this end, our group d

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Datta, Harish K., Iain MacIntyre, and Mone Zaidi. "The effect of extracellular calcium elevation on morphology and function of isolated rat osteoclasts." Bioscience Reports 9, no. 6 (December 1, 1989): 747–51. http://dx.doi.org/10.1007/bf01114813.

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Osteoclasts are large multinucleate cells unique in their capacity to resorb bone. These cells are exposed locally to high levels of ionised calcium during the process of resorption. We have therefore examined the effect of elevated extracellular calcium on the morphology and function of freshly disaggregated rat osteoclasts. Cell size and motility were quantitated by time-lapse video recording together with digitisation and computer-centred image analysis. In order to assess the resorptive capacity of isolated osteoclasts, we measured the total area of resorption of devitalised cortical bone

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Comes, Maria Colomba, Arianna Mencattini, Davide Di Giuseppe, Joanna Filippi, Michele D’Orazio, Paola Casti, Francesca Corsi, Lina Ghibelli, Corrado Di Natale, and Eugenio Martinelli. "A Camera Sensors-Based System to Study Drug Effects on In Vitro Motility: The Case of PC-3 Prostate Cancer Cells." Sensors 20, no. 5 (March 10, 2020): 1531. http://dx.doi.org/10.3390/s20051531.

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Cell motility is the brilliant result of cell status and its interaction with close environments. Its detection is now possible, thanks to the synergy of high-resolution camera sensors, time-lapse microscopy devices, and dedicated software tools for video and data analysis. In this scenario, we formulated a novel paradigm in which we considered the individual cells as a sort of sensitive element of a sensor, which exploits the camera as a transducer returning the movement of the cell as an output signal. In this way, cell movement allows us to retrieve information about the chemical compositio

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Tankersley, Richard A., and Ronald V. Dimock Jr. "Endoscopic visualization of the functional morphology of the ctenidia of the unionid mussel Pyganodon cataracta." Canadian Journal of Zoology 71, no. 4 (April 1, 1993): 811–19. http://dx.doi.org/10.1139/z93-106.

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During reproduction, the outer demibranchs of the unionid mussel Pyganodon cataracta serve as marsupia, with incubation of the developing shelled glochidia larvae occurring within the water tubes. In this study, recently developed endoscopic video analysis techniques were employed to examine in vivo the dynamics of filter feeding and water transport in mussels during gravid and postgravid periods. Particles entering the mantle cavity and retained by the gills were transported to the palps in a complex mucus-bound cord by the ventral food groove of the medial ctenidia. Larval incubation and cte

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Yang, Sam S., Elaine Yeh, E. D. Salmon, and Kerry Bloom. "Identification of a Mid-anaphase Checkpoint in Budding Yeast." Journal of Cell Biology 136, no. 2 (January 27, 1997): 345–54. http://dx.doi.org/10.1083/jcb.136.2.345.

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Activation of a facultative, dicentric chromosome provides a unique opportunity to introduce a double strand DNA break into a chromosome at mitosis. Time lapse video enhanced-differential interference contrast analysis of the cellular response upon dicentric activation reveals that the majority of cells initiates anaphase B, characterized by pole–pole separation, and pauses in mid-anaphase for 30–120 min with spindles spanning the neck of the bud before completing spindle elongation and cytokinesis. The length of the spindle at the delay point (3–4 μm) is not dependent on the physical distance

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