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Journal articles on the topic 'TIRF'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Treves, Susan, and Francesco Zorzato. "TIRF." Imaging & Microscopy 11, no. 3 (2009): 52–53. http://dx.doi.org/10.1002/imic.200990065.

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2

Truskey, G. A., J. S. Burmeister, E. Grapa, and W. M. Reichert. "Total internal reflection fluorescence microscopy (TIRFM). II. Topographical mapping of relative cell/substratum separation distances." Journal of Cell Science 103, no. 2 (1992): 491–99. http://dx.doi.org/10.1242/jcs.103.2.491.

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A simplified model of TIRF optics was used to quantitate the relative membrane/substratum separation distances from the spatial pattern of TIRF image brightness. Phase-contrast and total internal reflection fluorescence microscopy (TIRFM) images were collected of bovine aortic endothelial cells (BAEC) plated onto glass microscope slides for 15 min, 30 min and 24 h. BAEC adherent for 15 min showed an absence of a focal contact morphology, with the region of closest apposition beneath the cell center. After 30 min, multiple contacts with the surface were established and the morphology became mor

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Fu, Yan, Peter W. Winter, Raul Rojas, Victor Wang, Matthew McAuliffe, and George H. Patterson. "Axial superresolution via multiangle TIRF microscopy with sequential imaging and photobleaching." Proceedings of the National Academy of Sciences 113, no. 16 (2016): 4368–73. http://dx.doi.org/10.1073/pnas.1516715113.

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We report superresolution optical sectioning using a multiangle total internal reflection fluorescence (TIRF) microscope. TIRF images were constructed from several layers within a normal TIRF excitation zone by sequentially imaging and photobleaching the fluorescent molecules. The depth of the evanescent wave at different layers was altered by tuning the excitation light incident angle. The angle was tuned from the highest (the smallest TIRF depth) toward the critical angle (the largest TIRF depth) to preferentially photobleach fluorescence from the lower layers and allow straightforward obser

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Foylan, S., W. B. Amos, J. Dempster, et al. "MesoTIRF: A prism-based Total Internal Reflection Fluorescence illuminator for high resolution, high contrast imaging of large cell populations." Applied Physics Letters 122, no. 11 (2023): 113701. http://dx.doi.org/10.1063/5.0133032.

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Total internal reflection fluorescence (TIRF) illumination bypasses the axial diffraction limit of light by using an evanescent field to excite fluorophores close to a sample substrate. However, standard TIRF imaging through the objective requires a high numerical aperture (NA) to generate the evanescent wave. Available lenses have a high magnification with a correspondingly small field of view—ranging from [Formula: see text]50 μm to 1 mm in diameter. Switching to the older prism-TIRF configuration introduced by Axelrod in the 1980s might seem to remove the requirement for high objective NA a

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Punjabi, Gary, and Elena Ramírez. "Appropriateness of Prescribing Transmucosal Immediate-Release Fentanyl in the Emergency Room, During Hospitalization, and at Discharge: A Retrospective Study." Pharmaceuticals 17, no. 12 (2024): 1609. http://dx.doi.org/10.3390/ph17121609.

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Background/Objectives: This study evaluated the appropriateness of transmucosal immediate-release fentanyl (TIRF) prescriptions in a Madrid emergency room during 2019 and 2022, following a 2018 warning about off-label use. Methods: TIRF prescription in the emergency room search yielded 993 patients in 2019 and 1499 in 2022, of which 140 were randomized for the study, 70 in 2019, and 70 in 2022. Dose appropriateness and indication for TIRF were analyzed according to established criteria. Results: Despite a high prevalence of cancer diagnoses (77.9%, 109/140), only 32.9% (46/140) of patients met

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Lin, Jia, and Adam D. Hoppe. "Uniform Total Internal Reflection Fluorescence Illumination Enables Live Cell Fluorescence Resonance Energy Transfer Microscopy." Microscopy and Microanalysis 19, no. 2 (2013): 350–59. http://dx.doi.org/10.1017/s1431927612014420.

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AbstractFluorescence resonance energy transfer (FRET) microscopy is a powerful technique to quantify dynamic protein-protein interactions in live cells. Total internal reflection fluorescence (TIRF) microscopy can selectively excite molecules within about 150 nm of the glass-cell interface. Recently, these two approaches were combined to enable high-resolution FRET imaging on the adherent surface of living cells. Here, we show that interference fringing of the coherent laser excitation used in TIRF creates lateral heterogeneities that impair quantitative TIRF-FRET measurements. We overcome thi

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Zheng, Xiu, Xiaomian Cai, Wenjie Liu, Youhua Chen, and Cuifang Kuang. "Alternative Methods to Enhance the Axial Resolution of Total Internal Reflection Fluorescence–Structured Illumination Microscopy." Photonics 12, no. 7 (2025): 652. https://doi.org/10.3390/photonics12070652.

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Total internal reflection fluorescence–structured illumination microscopy (TIRF-SIM) can enhance the lateral resolution of fluorescence microscopy to twice the diffraction limit, enabling subtler observations of activity in subcellular life. However, the lack of an axial resolution makes it difficult to resolve three-dimensional (3D) subcellular structures. In this paper, we present an alternative TIRF-SIM axial resolution enhancement method by exploiting quantitative information regarding the distance between fluorophores and the surface within the evanescent field. Combining the lateral supe

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Lanni, F., A. S. Waggoner, and D. L. Taylor. "Structural organization of interphase 3T3 fibroblasts studied by total internal reflection fluorescence microscopy." Journal of Cell Biology 100, no. 4 (1985): 1091–102. http://dx.doi.org/10.1083/jcb.100.4.1091.

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We studied the laminar organization of 3T3 fibroblast cells growing on glass slides by use of total internal reflection illumination to excite fluorescence emission (TIRF) from labeled molecules and stained cellular compartments that are very close to the cell-substrate contact region. Mitochondria, distant from the contact regions and stained with the water-soluble cationic dye, dil-C3-(3), fluoresced only as the glass/cytoplasm critical angle was approached. A similar result was obtained when the nuclei were stained with Hoechst dye 33342. From this measured angle a cytoplasmic refractive in

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Morelo Pereira, Douglas Jahir, and Diocelina Torres Castro. "Técnicas e indicadores de rendimiento financiero aplicados al estado de resultados en empresas comerciales y de servicios colombianas." Cuadernos de Contabilidad 22 (August 19, 2021): 1–21. http://dx.doi.org/10.11144/javeriana.cc22.tirf.

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Este artículo está dirigido a identificar el grado de utilización de un conjunto de Técnicas e Indicadores de Rendimiento Financiero (TIRF) que permitan monitorear la sostenibilidad financiera de las empresas comerciales y de servicios. El problema radica en las frecuentes reformas tributarias y la constante lucha contra el contrabando; actividades que en su conjunto conjugan una amenaza a la sostenibilidad financiera de la empresa. En este sentido, la presente investigación busca analizar las características predominantes, diferenciales y correlacionales de las TIRF asociadas al estado de res

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Gingell, D., O. S. Heavens, and J. S. Mellor. "General electromagnetic theory of total internal reflection fluorescence: the quantitative basis for mapping cell-substratum topography." Journal of Cell Science 87, no. 5 (1987): 677–93. http://dx.doi.org/10.1242/jcs.87.5.677.

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Total internal reflection fluorescence (TIRF) has recently been used to look at the contacts made between cells and a glass surface on which they are spread. Our method utilizes the fluorescence of a water-soluble dye that acts as an extracellular aqueous volume marker. Fluorescence is stimulated by the short-range electric field near the glass surface that exists under conditions of total internal reflection. Since fluorescence is normally generated beneath a spread cell and not beyond it, the fluorescence of the image is related to the size of the cell-glass water gap. The images obtained ar

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Ellefsen, Kyle L., Joseph L. Dynes, and Ian Parker. "Spinning-Spot Shadowless TIRF Microscopy." PLOS ONE 10, no. 8 (2015): e0136055. http://dx.doi.org/10.1371/journal.pone.0136055.

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Balaa, Karla, Emmanuel Fort, and Nikon Instruments. "Surface Plasmon Enhanced TIRF Imaging." Imaging & Microscopy 11, no. 4 (2009): 55–56. http://dx.doi.org/10.1002/imic.200990091.

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Fairlamb, Max S., Amy M. Whitaker, Fletcher E. Bain, Maria Spies, and Bret D. Freudenthal. "Construction of a Three-Color Prism-Based TIRF Microscope to Study the Interactions and Dynamics of Macromolecules." Biology 10, no. 7 (2021): 571. http://dx.doi.org/10.3390/biology10070571.

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Single-molecule total internal reflection fluorescence (TIRF) microscopy allows for the real-time visualization of macromolecular dynamics and complex assembly. Prism-based TIRF microscopes (prismTIRF) are relatively simple to operate and can be easily modulated to fit the needs of a wide variety of experimental applications. While building a prismTIRF microscope without expert assistance can pose a significant challenge, the components needed to build a prismTIRF microscope are relatively affordable and, with some guidance, the assembly can be completed by a determined novice. Here, we provid

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Olevsko-Arad, Ilya, Moshe Feldberg, Martin Oheim, and Adi Salomon. "Colour-coded nanoscale calibration and optical quantification of axial fluorophore position." EPJ Web of Conferences 287 (2023): 09034. http://dx.doi.org/10.1051/epjconf/202328709034.

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Total internal reflection fluorescence (TIRF) has come of age, but a reliable and easy-to-use tool for calibrating evanescent-wave penetration depths is missing. We provide a test-sample for TIRF and other axial super-resolution microscopies for emitter axial calibration. Our originality is that nanometer(nm) distances along the microscope’s optical axis are color-encoded in the form of a multi-layered multi-colored transparent sandwich. Emitter layers are excited by the same laser but they emit in different colors. Layers are deposited in a controlled manner onto a glass substrate and protect

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Keffer, J. L., C. R. Sabanayagam, M. E. Lee, E. F. DeLong, M. W. Hahn, and J. A. Maresca. "Using Total Internal Reflection Fluorescence Microscopy To Visualize Rhodopsin-Containing Cells." Applied and Environmental Microbiology 81, no. 10 (2015): 3442–50. http://dx.doi.org/10.1128/aem.00230-15.

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ABSTRACTSunlight is captured and converted to chemical energy in illuminated environments. Although (bacterio)chlorophyll-based photosystems have been characterized in detail, retinal-based photosystems, rhodopsins, have only recently been identified as important mediators of light energy capture and conversion. Recent estimates suggest that up to 70% of cells in some environments harbor rhodopsins. However, because rhodopsin autofluorescence is low—comparable to that of carotenoids and significantly less than that of (bacterio)chlorophylls—these estimates are based on metagenomic sequence dat

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Knight, Alex E. "Single-molecule fluorescence imaging by total internal reflection fluorescence microscopy (IUPAC Technical Report)." Pure and Applied Chemistry 86, no. 8 (2014): 1303–20. http://dx.doi.org/10.1515/pac-2012-0605.

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AbstractTotal internal reflection fluorescence (TIRF) is a popular illumination technique in microscopy, with many applications in cell and molecular biology and biophysics. The chief advantage of the technique is the high contrast that can be achieved by restricting fluorescent excitation to a thin layer. We summarise the optical theory needed to understand the technique and various aspects required for a practical implementation of it, including the merits of different TIRF geometries. Finally, we discuss a variety of applications including super-resolution microscopy and high-throughput DNA

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Rüttinger, Steffen, Baptiste Lamarre, and Alex E. Knight. "Single Molecule Genotyping by TIRF Microscopy." Journal of Fluorescence 18, no. 5 (2008): 1021–26. http://dx.doi.org/10.1007/s10895-008-0386-2.

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Castell, Oliver K., James M. Berridge, and Mark I. Wallace. "TIRF Imaging of Lipid Bilayer Arrays." Biophysical Journal 100, no. 3 (2011): 317a. http://dx.doi.org/10.1016/j.bpj.2010.12.1932.

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Tonzani, Stefano. "TIRF: imaging at the cellular edge." Nature Cell Biology 11, S1 (2009): S16. http://dx.doi.org/10.1038/ncb1933.

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Greb, Christoph, Ralf Jacob, and Anja Schué. "TIRF-Mikroskopie auf den Kopf gestellt." BIOspektrum 17, no. 3 (2011): 314–16. http://dx.doi.org/10.1007/s12268-011-0050-2.

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Denham, Simon, and Deborah Cutchey. "Total Internal Reflection Fluorescence (TIRF) Microscopy." Imaging & Microscopy 11, no. 2 (2009): 54–55. http://dx.doi.org/10.1002/imic.200990043.

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Abramova, Natalia, Odeniel Sertil, Sapna Mehta, and Charles V. Lowry. "Reciprocal Regulation of Anaerobic and Aerobic Cell Wall Mannoprotein Gene Expression in Saccharomyces cerevisiae." Journal of Bacteriology 183, no. 9 (2001): 2881–87. http://dx.doi.org/10.1128/jb.183.9.2881-2887.2001.

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ABSTRACT The DAN/TIR genes encode nine cell wall mannoproteins in Saccharomyces cerevisiae which are expressed during anaerobiosis (DAN1,DAN2, DAN3, DAN4,TIR1, TIR2, TIR3,TIR4, and TIP1). Most are expressed within an hour of an anaerobic shift, but DAN2 andDAN3 are expressed after about 3 h. At the same time, CWP1 and CWP2, the genes encoding the major mannoproteins, are down-regulated, suggesting that there is a programmed remodeling of the cell wall in which Cwp1 and Cwp2 are replaced by nine anaerobic counterparts. TIP1,TIR1, TIR2, and TIR4 are also induced during cold shock. Correspondingl

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Jaykumar, Ankita Bachhawat, Paulo S. Caceres, Ibrahim Sablaban, Bakhos A. Tannous, and Pablo A. Ortiz. "Real-time monitoring of NKCC2 endocytosis by total internal reflection fluorescence (TIRF) microscopy." American Journal of Physiology-Renal Physiology 310, no. 2 (2016): F183—F191. http://dx.doi.org/10.1152/ajprenal.00104.2015.

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The apical Na-K-2Cl cotransporter (NKCC2) mediates NaCl reabsorption by the thick ascending limb (TAL). The amount of NKCC2 at the apical membrane of TAL cells is determined by exocytic delivery, recycling, and endocytosis. Surface biotinylation allows measurement of NKCC2 endocytosis, but it has low time resolution and does not allow imaging of the dynamic process of endocytosis. We hypothesized that total internal reflection fluorescence (TIRF) microscopy imaging of labeled NKCC2 would allow monitoring of NKCC2 endocytosis in polarized Madin-Darby canine kidney (MDCK) and TAL cells. Thus we

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Park, Young IL, Seung Ha Lee, and Jeong-Hwan Kim. "Study of Applicability of Triangular Impulse Response Function for Ultimate Strength of LNG Cargo Containment Systems under Sloshing Impact Loads." Applied Sciences 13, no. 5 (2023): 2883. http://dx.doi.org/10.3390/app13052883.

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The LNG cargo containment system used in membrane-type LNG cargo tanks must have sufficient dynamic strength to withstand the impact of sloshing loads. However, performing direct dynamic nonlinear transient finite element assessments against design sloshing impact loads with different design specifications can be complicated and time-consuming. To address this, it is effective to use linear superposition methods, such as the triangular impulse response function (TIRF) method, to conduct dynamic transient FE assessments of LNG cargo containment systems. However, as LNG cargo containment systems

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Young, Laurence J., Gabriele S. Kaminski Schierle та Clemens F. Kaminski. "Imaging Aβ(1–42) fibril elongation reveals strongly polarised growth and growth incompetent states". Phys. Chem. Chem. Phys. 19, № 41 (2017): 27987–96. http://dx.doi.org/10.1039/c7cp03412a.

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Laasfeld, Tõnis, Robin Ehrminger, Maris-Johanna Tahk, et al. "Budded baculoviruses as a receptor display system to quantify ligand binding with TIRF microscopy." Nanoscale 13, no. 4 (2021): 2436–47. http://dx.doi.org/10.1039/d0nr06737g.

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Drawbond, Rachel, та Kathrin Spendier. "TIRF Microscope Image Sequences of Fluorescent IgE-FcεRI Receptor Complexes inside a FcεRI-Centric Synapse in RBL-2H3 Cells". Data 4, № 3 (2019): 111. http://dx.doi.org/10.3390/data4030111.

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Total internal reflection fluorescence (TIRF) microscope image sequences are commonly used to study receptors in live cells. The dataset presented herein facilitates the study of the IgE-FcεRI receptor signaling complex (IgE-RC) in rat basophilic leukemia (RBL-2H3) cells coming into contact with a supported lipid bilayer with 25 mol% N-dinitrophenyl-aminocaproyl phosphatidylethanolamine, modeling an immunological synapse. TIRF microscopy was used to image IgE-RCs within this FcεRI-centric synapse by loading RBL-2H3 cells with fluorescent anti-dinitrophenyl (anti-DNP) immunoglobulin E (IgE) in

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Boguslavsky, Shlomit, Tim Chiu, Kevin P. Foley, et al. "Myo1c binding to submembrane actin mediates insulin-induced tethering of GLUT4 vesicles." Molecular Biology of the Cell 23, no. 20 (2012): 4065–78. http://dx.doi.org/10.1091/mbc.e12-04-0263.

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GLUT4-containing vesicles cycle between the plasma membrane and intracellular compartments. Insulin promotes GLUT4 exocytosis by regulating GLUT4 vesicle arrival at the cell periphery and its subsequent tethering, docking, and fusion with the plasma membrane. The molecular machinery involved in GLUT4 vesicle tethering is unknown. We show here that Myo1c, an actin-based motor protein that associates with membranes and actin filaments, is required for insulin-induced vesicle tethering in muscle cells. Myo1c was found to associate with both mobile and tethered GLUT4 vesicles and to be required fo

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Won, Jong Hak, and David I. Yule. "Measurement of Ca2+ signaling dynamics in exocrine cells with total internal reflection microscopy." American Journal of Physiology-Gastrointestinal and Liver Physiology 291, no. 1 (2006): G146—G155. http://dx.doi.org/10.1152/ajpgi.00003.2006.

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In nonexcitable cells, such as exocrine cells from the pancreas and salivary glands, agonist-stimulated Ca2+ signals consist of both Ca2+ release and Ca2+ influx. We have investigated the contribution of these processes to membrane-localized Ca2+ signals in pancreatic and parotid acinar cells using total internal reflection fluorescence (TIRF) microscopy (TIRFM). This technique allows imaging with unsurpassed resolution in a limited zone at the interface of the plasma membrane and the coverslip. In TIRFM mode, physiological agonist stimulation resulted in Ca2+ oscillations in both pancreas and

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Machado, Bendesky, Brown, Spendier та Hagen. "Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation". Journal of Imaging 5, № 7 (2019): 63. http://dx.doi.org/10.3390/jimaging5070063.

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Total internal reflection fluorescence microscopy with polarized excitation (P-TIRF) can be used to image nanoscale curvature phenomena in live cells. We used P-TIRF to visualize rat basophilic leukemia cells (RBL-2H3 cells) primed with fluorescent anti-dinitrophenyl (anti-DNP) immunoglobulin E (IgE) coming into contact with a supported lipid bilayer containing mobile, monovalent DNP, modeling an immunological synapse. The spatial relationship of the IgE-bound high affinity IgE receptor (FcεRI) to the ratio image of P-polarized excitation and S-polarized excitation was analyzed. These studies

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Fu, Meifang, Luru Dai, Qiao Jiang, et al. "Observation of intracellular interactions between DNA origami and lysosomes by the fluorescence localization method." Chemical Communications 52, no. 59 (2016): 9240–42. http://dx.doi.org/10.1039/c6cc00484a.

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Rangnekar, Vinay M., John T. Foley, and Philip B. Oldham. "Investigation of Chromatographic Surface Viscosity Using Total Internal Reflection Fluorescence." Applied Spectroscopy 46, no. 5 (1992): 827–31. http://dx.doi.org/10.1366/0003702924124600.

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Total internal reflection fluorescence (TIRF) spectroscopy was employed to determine the viscosity of planar chromatographic surfaces. Various n-alkyldimethylchlorosilanes were used to modify the surface of a UV-quartz plate, so as to obtain a covalently attached monomeric C1, C3, C8, or C18 surface. TIRF anisotropy data of 1, 6-diphenylhexatriene (DPH) were collected on the unmodified and modified surfaces in the presence of overlaying solvents covering a range of viscosities. The results indicate that DPH adsorbs onto C1 and C3, but clearly exhibits partitioning with the C18 phase. The C8 su

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Liras, M., S. Simoncelli, A. Rivas-Aravena, et al. "Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy." Organic & Biomolecular Chemistry 14, no. 17 (2016): 4023–26. http://dx.doi.org/10.1039/c6ob00533k.

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Richter, Verena, Peter Lanzerstorfer, Julian Weghuber, and Herbert Schneckenburger. "Super-Resolution Live Cell Microscopy of Membrane-Proximal Fluorophores." International Journal of Molecular Sciences 21, no. 19 (2020): 7099. http://dx.doi.org/10.3390/ijms21197099.

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Here, we present a simple and robust experimental setup for the super-resolution live cell microscopy of membrane-proximal fluorophores, which is comparably easy to perform and to implement. The method is based on Structured Illumination Microscopy (SIM) with a switchable spatial light modulator (SLM) and exchangeable objective lenses for epi-illumination and total internal reflection fluorescence (TIRF) microscopy. While, in the case of SIM (upon epi-illumination), cell layers of about 1–2 µm in close proximity to the plasma membrane can be selected by software, layers in the 100 nm range are

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Mantil, Elisabeth, Trinda Crippin, Anatoli Ianoul, and Tyler J. Avis. "Experimental Parameters Leading to Optimal Bilayers for Total Internal Reflection Fluorescence Microscopy Visualization." Microscopy and Microanalysis 23, no. 1 (2017): 97–112. http://dx.doi.org/10.1017/s1431927617000083.

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AbstractSupported lipid bilayer systems were evaluated following various experimental procedures in an effort to determine their appropriateness for visualization using total internal reflection fluorescence (TIRF) microscopy. The incorporation and distribution of Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (TR-DHPE) was studied when incorporated into bilayers of variable lipid composition using different forms of mechanical shearing. Results showed that 0.8 mol% TR-DHPE provides the most optimum TIRF images. At this concentration, a sufficient level of photostability can be

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Nishizaka, T., T. Okuno, J. Okawa, et al. "Exploration and application of innovative TIRF microscopy." Seibutsu Butsuri 43, supplement (2003): S229. http://dx.doi.org/10.2142/biophys.43.s229_3.

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Shen, Hao, Eric Huang, Tapaswini Das, Hongxing Xu, Mark Ellisman, and Zhaowei Liu. "TIRF microscopy with ultra-short penetration depth." Optics Express 22, no. 9 (2014): 10728. http://dx.doi.org/10.1364/oe.22.010728.

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Tedeschi, Lorena, Claudio Domenici, Arti Ahluwalia, Francesco Baldini, and Andrea Mencaglia. "Antibody immobilisation on fibre optic TIRF sensors." Biosensors and Bioelectronics 19, no. 2 (2003): 85–93. http://dx.doi.org/10.1016/s0956-5663(03)00173-8.

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Popescu, Alina L., Kathryn K. Gersh, Dan Safer, and John W. Weisel. "Single Molecule TIRF Study of Fibrinogen Polymerization." Biophysical Journal 100, no. 3 (2011): 153a. http://dx.doi.org/10.1016/j.bpj.2010.12.1049.

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Hoying, James B., and Stuart K. Williams. "Endothelial cell monolayers viewed by TIRF microscopy." In Vitro Cellular & Developmental Biology - Animal 30, no. 1 (1994): 1–3. http://dx.doi.org/10.1007/bf02631406.

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Zong, Weijian, Xiaoshuai Huang, Chi Zhang, et al. "Shadowless-illuminated variable-angle TIRF (siva-TIRF) microscopy for the observation of spatial-temporal dynamics in live cells." Biomedical Optics Express 5, no. 5 (2014): 1530. http://dx.doi.org/10.1364/boe.5.001530.

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Мочалов, К. Е., О. И. Агапова, И. И. Агапов та ін. "ФЛУОРЕСЦЕНТНАЯ ОПТИЧЕСКАЯ НАНОТОМОГРАФИЯ: ОБЪЕДИНЕНИЕ ТЕХНИК ФЛУОРЕСЦЕНТНОЙ МИКРОСКОПИИ ПОЛНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ И УЛЬТРАМИКРОТОМИИ". Nanoindustry Russia 17, № 7-8 (2024): 428–33. http://dx.doi.org/10.22184/1993-8578.2024.17.7-8.428.433.

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Предложен метод 3D-TIRF-микроскопии, основанный на объединении методик ультрамикротомии и флуоресцентной микроскопии полного внутреннего отражения поверхности образца после среза и позволяющий реконструировать трехмерную ультраструктуру объектов.

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Soni, Gautam V., and Amit Meller. "Progress toward Ultrafast DNA Sequencing Using Solid-State Nanopores." Clinical Chemistry 53, no. 11 (2007): 1996–2001. http://dx.doi.org/10.1373/clinchem.2007.091231.

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Abstract Background: Measurements of the ionic current flowing through nanometer-scale pores (nanopores) have been used to analyze single DNA and RNA molecules, with the ultimate goal of achieving ultrafast DNA sequencing. However, attempts at purely electronic measurements have not achieved the signal contrast required for single nucleotide differentiation. In this report we propose a novel method of optical detection of DNA sequence translocating through a nanopore. Methods: Each base of the target DNA sequence is 1st mapped onto a 2-unit code, 2 10-bp nucleotide sequence, by biochemical con

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Reichert, W. M., and G. A. Truskey. "Total internal reflection fluorescence (TIRF) microscopy. I. Modelling cell contact region fluorescence." Journal of Cell Science 96, no. 2 (1990): 219–30. http://dx.doi.org/10.1242/jcs.96.2.219.

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Total Internal Reflection Fluorescence (TIRF) is a powerful technique for visualizing focal and close contacts between the cell and the surface. Practical application of TIRF has been hampered by the lack of straightforward methods to calculate separation distances. The characteristic matrix theory of thin dielectric films was used to develop simple exponential approximations for the fluorescence excited in the cell-substratum contact region during a TIRF experiment. Two types of fluorescence were examined: fluorescently labeled cell membranes, and a fluorescent water-soluble dye. By neglectin

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Peng, Weichen, Luo Chen, Xue Ouyang, and Wei Xiong. "A Time-Identified R-Tree: A Workload-Controllable Dynamic Spatio-Temporal Index Scheme for Streaming Processing." ISPRS International Journal of Geo-Information 13, no. 2 (2024): 49. http://dx.doi.org/10.3390/ijgi13020049.

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Many kinds of spatio-temporal data in our daily lives, such as the trajectory data of moving objects, stream natively. Streaming systems exhibit significant advantages in processing streaming data due to their distributed architecture, high throughput, and real-time performance. The use of streaming processing techniques for spatio-temporal data applications is a promising research direction. However, due to the strong dynamic nature of data in streaming processing systems, traditional spatio-temporal indexing techniques based on relatively static data cannot be used directly in stream-process

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46

Reichert, W. M., P. A. Suci, J. T. Ives, and J. D. Andrade. "Evanescent Detection of Adsorbed Protein Concentration-Distance Profiles: Fit of Simple Models to Variable-Angle Total Internal Reflection Fluorescence Data." Applied Spectroscopy 41, no. 3 (1987): 503–8. http://dx.doi.org/10.1366/0003702874448913.

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Total internal reflection fluorescence spectroscopy (TIRF) is an established technique for following the course of interfacial reactions. Theoretically, by gathering TIRF data as a function of observation angle, one can obtain the density of fluorophores with respect to distance away from a solid/liquid interface. In order that the practical application of the theory might be explored, variable observation angle data from solutions of fluorescein and from adsorbed layers of fluorescein isothio-cyanate labeled immunoglobulin have been analyzed in terms of simple concentration-distance profiles.

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Matsui, K. "Optical aberration correction for TIRF single molecule imaging." Seibutsu Butsuri 43, supplement (2003): S17. http://dx.doi.org/10.2142/biophys.43.s17_1.

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Hingorani, Manju M. "TIRF(ing) reveals Msh2-Msh6 surfing on DNA." Nature Structural & Molecular Biology 14, no. 12 (2007): 1124–25. http://dx.doi.org/10.1038/nsmb1207-1124.

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Holden, Seamus J., Stephan Uphoff, David Yadin, et al. "TIRF-Based FRET with One Base-Pair Resolution." Biophysical Journal 98, no. 3 (2010): 413a. http://dx.doi.org/10.1016/j.bpj.2009.12.2230.

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Lee, Jinwoo, and Sungchul Hong Hohng. "Alex Single Molecule Three Color in TIRF Microscopy." Biophysical Journal 98, no. 3 (2010): 747a. http://dx.doi.org/10.1016/j.bpj.2009.12.4096.

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