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Cubbin, Ian James. "Tissue culture studies in selected medicinal plants." Thesis, University of Sunderland, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320538.
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Yamagishi, Masumi. "Genetic evaluation of cell and tissue culture-derived rice plants." Kyoto University, 1997. http://hdl.handle.net/2433/202414.
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Marlow, Susan A. "Acclimatization physiology in tissue cultured plants." Thesis, Oxford Brookes University, 1990. https://radar.brookes.ac.uk/radar/items/a1e3ad31-39e0-4cd1-b236-7ae638edcdf7/1/.
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Physiological and morphological aspects of acclimatization were studied in cultured tomato (Lycopersicon esculentum Mill.), banana (Musa accuminata L.) and date palm (Phoenix dactyli/era ). The nutrient availability from agar solidified culture medium was determined to establish the nutrient status of the cultured plandets before transfer to ex vitro conditions. Analysis of the plant tissues demonstrated decreasing tissue concentrations of the major elements nitrogen, phosphorus and potassium with decreasing concentration of basal salts in the medium. The effects of agar and increasing sodium concentration in the culture medium was studied in cultured banana plants. Plandets grown on agar solidified medium with increased levels of sodium, exhibited reduced growth and stomatal movement. The use of agar as a solidifying agent was shown to reduce root growth, development and stomatal functioning in these plants. The efficiency of ion and water uptake, and translocation in in vitro and acclimatized tomato plants was assessed using [32P]-orthophosphate and [3H]_ tritiated water. The functional capacity of the root system fOlmed in vitro was established, and assessed following acclimatization treatments at 40% and 80% relative humidity. Comparative studies with tomato seedlings demonstrated reduced efficiency of ion translocation to the shoot in plandets growing in vitro. However, transport to the shoot improved during acclimatization. Ion absorption studies on in vitro and acclimatized palm plants demonstrated phosphate uptake and translocation in both plant types. A detailed examination of the tissue structure through the root/shoot junction and roots of · cultured, acclimatized and seedling tomato plants illustrated differences in the vascular development between the three plant types. However, no major abnormalities were observed which could have accounted for the reduced translocation efficiency in the cultured plants. Increased vascularization present in the root/shoot junction of the cultured plants may increase resistance to the transpiration flow through the region. The type of root system produced in vitro and the root/shoot ratio was manipulated using varying IAA and sucrose treatments. Improved root development and plantlet survival rates were achieved by reduced exposure to IAA during the root initiation phase followed by root elongation on IAA free medium supplemented with sucrose. Acclimatization at low relative humidity (40%) was achieved by producing plandets with balanced root/shoot ratios and a well developed root system.
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Shih, Sharon Min-Hsuan Biotechnology & Biomolecular Sciences Faculty of Science UNSW. "Transient viral infection of plant tissue culture and plants for production of virus and foreign protein." Awarded by:University of New South Wales, 2007. http://handle.unsw.edu.au/1959.4/34967.
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This work was aimed to investigate the basic viral infection protocols mainly focusing on Nicotiana benthamiana hairy root cultures and wild-type tobacco mosaic virus (TMV). The application of transgenic virus containing the gene for green fluorescent protein (GFP) for foreign protein production in plant tissue cultures and whole plants was also studied. The effect on viral accumulation of the form of plant tissue culture used, such as hairy roots, shooty teratomas and suspended cells, was investigated. Viral infection was shown to have no effect on culture growth and morphology. Hairy root cultures are a superior host for viral propagation and production in vitro. The maximum specific rate of viral accumulation occurred mainly during the root growth phase. The average maximum virus concentration in the hairy roots was 0.82 ?? 0.14 mg g-1 dry weight and virus protein represented a maximum of approximately 6% of total soluble protein in the root biomass. Proportional scale-up of TMVinfected hairy roots in shake flasks and bioreactors can be achieved without changing the average virus concentration accumulated in the hairy roots. The level of viral accumulation was much lower in N. benthamiana hairy roots infected with transgenic virus containing GFP (TMVGFPC3) compared with TMV and low levels or no GFP was detected. Viral accumulation and GFP production in whole plants was studied using different generations of transgenic TMV-GFPC3 virus. Hybrid viruses with the foreign gene GFPC3 deleted may have been formed in successive TMV-GFPC3 generations, resulting in the loss of GFP production and enhanced viral infectivity. In vitro generated RNA transcript and first generation TMV-GFPC3 were found to be more suitable for infection than the second generation TMV-GFPC3. However, the accumulation of GFP and virus concentration did not occur at the same ratio. Provided a more genetically stable transgenic viral vector is used for infection, transient viral infection of hairy roots can be a potential alternative system for foreign protein production than plants grown in the field as the containment or safety issues can be addressed.
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Slomka, Marek Jozef. "Studies of tomato golden mosaic virus in plants, protoplasts and tissue culture." Thesis, Imperial College London, 1987. http://hdl.handle.net/10044/1/46616.
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Ubaid, R. H. "Plant tissue culture and prostaglandin production from a range of Allium species." Thesis, University of Salford, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381734.
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Sheibani, Ahmad. "Tissue culture studies of Pistacia." Thesis, University of Salford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.238801.
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Gazis, Fotis. "Forsythia X intermedia cv. spectabilis (Zab.) in vitro propagation and raspberry ringspot virus elimination." Thesis, University of Bath, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334617.
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Gribble, Karleen Dawn. "Towards an understanding of the physiological abnormality of tissue cultured plants known as vitrification /." [Richmond, N.S.W.] : Horticulture, University of Western Sydney, Hawkesbury, 1999. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030513.144109/index.html.
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Thesis (Ph.D.) -- University of Western Sydney, Hawkesbury, 1999.Thesis submitted for the degree of Doctor of Philosophy. Spine title: Towards an understanding of vitrification in tissue cultured plants. Includes bibliographical references (leaves 175-203).
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Al-Ani, Nabeel K. "Some epigenetic effects in plant tissue culture." Thesis, Aberystwyth University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.659362.
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Al-Shakarchi, E. M. D. "Transformation of sterols in plant tissue cultures." Thesis, Bucks New University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384187.
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Viegas, Bruno Miguel Fragoso. "Utilisation of tissue culture techniques and induced mutations for propagation and breeding of ornamental plants." Thesis, University of Birmingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289290.
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James, V. J. "Regulation of xenobiotic catabolism in plant tissue culture." Thesis, Cardiff University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380205.
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Ahmed, Eakhlas Uddin. "Development of micro-propagation procedure in Caladium bicolor plants-True-type-plant propagation and a new simplified tissue culture method." Kyoto University, 2003. http://hdl.handle.net/2433/148976.
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Kyoto University (京都大学)0048
新制・課程博士
博士(農学)
甲第10249号
農博第1321号
新制||農||865(附属図書館)
学位論文||H15||N3770(農学部図書室)
UT51-2003-H670
京都大学大学院農学研究科農学専攻
(主査)教授 矢澤 進, 教授 櫻谷 哲夫, 教授 米森 敬三
学位規則第4条第1項該当
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Gribble, Karleen D., of Western Sydney Hawkesbury University, and Faculty of Science and Technology. "Towards an understanding of the physiological abnormality of tissue cultured plants known as vitrification." THESIS_FST_HPS_Gribble_K.xml, 1999. http://handle.uws.edu.au:8081/1959.7/417.
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For this research, the abnormality of tissue cultured plantlets,vitrification, was examined in Gypsophila paniculata.Measurement of the relative water content and water saturation deficit of plantlets in culture revealed that vitrified plantlets contain relatively more water and less air spaces than non-vitrified plantlets.The effect of relative humidity on vitrification and growth was investigated using a variety of methods.From the results found, it was determined the defining characteristic of vitrified plantlets is water filled intercellular spaces. It was also determined that the primary cause of vitrification is high relative humidity resulting in a lack of transpiration in vitro but that other factors such as unbalanced mineral nutrition or high medium cytokinin can exacerbate vitrification.Further research in tissue culture may investigate the influence of relative humidity on plant growth and morphology, the mechanism by which plants exclude water from their intercellular spaces and refine in vitro tissue mineral analysis as a means by which critical mineral concentrations can be determined.Doctor of Philosophy (PhD)
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Borda, Yepez Charlotte Cesty [UNESP]. "Potencial morfogenético e carotenóides em tecidos cultivados in vitro de Pothomorphe umbellata L." Universidade Estadual Paulista (UNESP), 2003. http://hdl.handle.net/11449/103306.
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bordayepez_cc_me_botfca.pdf: 605083 bytes, checksum: 56a87160ec1d63940d534d9e6a51cff7 (MD5)O presente trabalho teve como objetivo estabelecer o protocolo de desinfestação de estacas e sernentes e germinação de Pothomorphe umbeilata (L) e adaptar o protocolo de micropropagação de Pothomorphe umbeilata (L), incluindo a produção de calos e organogênese, além de comparar o teor de carotenóides entre calos e plântulas. O experimento foi conduzido no Laboratório de Biotecnologia Vegetal do Departamento de Química e Bioquímica, do Instituto de Biociências da UNESP - Botucatu, SP e o material vegetal (sementes e estacas) foi obtido no município de Adrianópolis-PR. Semente germinadas de P. umbeilata foram inoculadas em diferentes concentrações de BAP (0,5 mg.L ; 1,0 mgL ; 1,5 mg.L) e NAA (0,4 mg.L 0,6 mg.L ; 0,6 mg.L ) respectivamente, visando estimular a produção de calos e dosar o carotenóides. Após 60 dias do cultivo, os calos contendo algumas brotações, foram transferidos para meio de diferenciação das plântulas (GA3 0,1 mg.L , BAP 0,5 mgL ) por um período de 40 dias, para logo serem transferidos para meio de diferenciação de plântulas. Calos (coletados aos 60 dias) e plântulas (coletadas aos 140 dias) foram congelados em nitrogênio líquido e mantidos em freezer a 80°C para posteriores análises de carotenóides. O melhor tratamento para a produção de calos e formação de gemas foi NAA 0.6 mgL em combinação com BAP 10 mg.L . Nas plântulas sem adição de reguladores vegetais foi encontrada uma maior concentração de carotenõides, em comparação aos calos.
The present research aimed at establishing a protocol of stalks and seed desinfectation, and seed germination of Pothomorphe umbeilata (L.). A protocol of micropropagation including the induction of callus formation and organogenesis was adapted. Besides, it was compared the carotenoids quantity between Porhomorphe umbeliata calius and plantlets. This experiment was carried out iii Biotechnology Vegetal Laboratory of the Chernistr and Biochemistry Department at the Instituto de Biociências of Universidade Estadual Paulista, Botucatu campus. The vegetal matenal (seed and stalks) was obtained from Adranópolis - PR. Pothomorphe umbeilata germinated seeds were inoculated in different concentrations of BAP (0,5 mg.L-l; 1,0 mg.L.-l; 1,5 mg.L-l) and NAA (0,4 mg.L-l; 0,6 mg.L-1; 0,6 mg.L-l) respectively, in order to stimulate calius induction and increase quantity of earotenoids. Afler 60 days, calius which contained shoots were inoculated in plantlets diferenciation medium GA3 0,1 mg.L4, BAP 0,5 mg.U ) during 40 days and transferred to plantlets growth medium. Plantlets were acclimatized Calius collected after 60 days and plantlets were collected afier 140 days. There were frozen in liquid nitrogen and maintained in freezer 80°C to be used in further carotenoids test. The best treatment for callus production and shoots elongation was NAA 0.6 mg.L in association with BAP 1.0 mg.L . The higher carotenoids coneentration was in plantlets without growth regulators, compared with calius.
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Essa, A. K. "Variations in alkaloidal constituents of plant tissue cultures." Thesis, University of Manchester, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379163.
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Silva, Lívia Cristina da. "Germinação, estabelecimento e multiplicação in vitro de Eugenia dysenterica DC. e Dipteryx alata Vogel, espécies frutíferas do cerrado." Universidade Federal de Goiás, 2012. http://repositorio.bc.ufg.br/tede/handle/tede/4967.
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Dipteryx alata Vogel and Eugenia dysenterica DC. are Cerrado’s fruit tree threatened by habitat fragmentation and the predatory extractivism. Thus, it is essential to the study of techniques for the conservation and sustainable use of these species. The objective for this work was to establish protocols for micropropagation of these species from the in vitro germination of their seeds. Experiments were conducted at the Laboratory of Plant Tissue Culture ICB / UFG. Seeds of both species were divided into two groups: with coat and without coat. After pre-cleansing with detergent and alcohol 70%, seeds of D. alata were treated with four concentrations of sodium hypochlorite. Seeds of E. dysenterica were treated with four concentrations of sodium hypochlorite and three of casugamicina. The seeds were inoculated in ½ MS and MS complete, with or without addition of charcoal. The lowest contamination percentage for E. dysenterica occurred with seeds without tegument soaked in 0.5% of active chlorine. To D. alata, the most effective treatment was with seed-coats soaked in 1.25% of active chlorine. For the germination of E. dysenterica, the seed coats were removed. The treatments used through complete MS and ½ MS. After inoculation, the seeds remained in a growth chamber in two distinct photoperiods: 16 h light or 24 hours of dark. The highest germination percentage for E. dysenterica, with 93%, occurred in complete MS medium in a 16h photoperiod. To D. alata, the highest germination percentage, 97.5%, occurred in complete MS medium without charcoal, in a 16h photoperiod. To verify the induction of shoots of both species, nodal segments were inoculated on MS medium supplemented with different concentrations of NAA and BAP, the best treatment for multiplication of shoots in E dysenterica was with 4.0 mg.L-1 BAP. For D. alata, the best was 0.1 mg.L-1 NAA and 2.5 mg l-1 BAP. To study the roots and shoots from in vitro cultures inoculated in a MS or ½ MS supplemented with combinations of NAA, sucrose and activated charcoal. No satisfactory results occurred for rooting in any species. For E. dysenterica, the few seedlings that emitted roots did not stand the acclimatization. To D. alata, no emission of roots was detected, but there was the issue of shoots in all treatments, especially those inoculated in ½ MS. To obtain the callus, stem explants of D. alata, and leaf explants of E. dysenterica were inoculated on MS medium supplemented with different concentrations of BAP and 2.4 D. As a result, we obtained callus formation in D. alata with BAP, ranging from 0.0 mg L-1 to 1.0 mg.L-1, interacting with 2,4-D, ranging from 1.0 mg L-1 and 4.0 mg L-1. Leaf explants of E. dysenterica callus was obtained between 3.0 and 4.0 mg.L-1 of 2.4-D combined with 0.5 and 1.0 mg.L-1 BAP.
Dipteryx alata Vogel e Eugenia dysenterica DC. são frutíferas do Cerrado ameaçadas pela fragmentação de seu habitat e pelo extrativismo predatório. Deste modo, torna-se imprescindível o estudo de técnicas para a conservação e uso sustentável destas espécies. O objetivo do trabalho foi estabelecer protocolos de micropropagação destas espécies a partir da germinação in vitro. Experimentos foram conduzidos no Laboratório de Cultura de Tecidos Vegetais do ICB/UFG. Sementes das duas espécies foram divididas em dois grupos: com tegumento e sem tegumento. Após pré-assepsia com detergente e álcool 70%, as sementes de D. alata foram tratadas com quatro concentrações de hipoclorito de sódio. Sementes de E. dysenterica foram tratadas com quatro concentrações de hipoclorito de sódio e três de casugamicina. As sementes foram inoculadas em meio MS completo e ½ MS, com ou sem adição de carvão. A menor porcentagem de contaminação para E. dysenterica foi com sementes sem tegumento, imersas em 0,5% de cloro ativo. Para D. alata, o tratamento mais eficiente foi com sementes com tegumento imersas em 1,25% de cloro ativo. Para a germinação de sementes de E. dysenterica, os tegumentos foram removidos. Os tratamentos utilizaram meio MS completo e ½ MS. Após a inoculação, as sementes permaneceram em sala de crescimento em dois fotoperíodos distintos: 16 horas de claro ou 24 horas de escuro. A maior porcentagem de germinação para E. dysenterica, com 93%, ocorreu em meio MS completo no fotoperíodo de 16h. Para D. alata, a maior porcentagem de germinação ocorreu em meio MS completo, sem carvão, no fotoperíodo de 16h com 97,5%. Para verificar a indução de brotações das duas espécies, segmentos nodais foram inoculados em meio MS suplementado com diferentes concentrações e combinações de ANA e BAP, O melhor tratamento para multiplicação de brotações em E. dysenterica foi com 4,0 mg.L-1 de BAP. Já para D. alata o melhor foi 0,1 mg.L-1 de ANA e 2,5 mg.L-1 de BAP. Para o estudo do enraizamento, brotações provenientes do cultivo in vitro inoculadas em meio de MS ou ½ MS suplementado com combinações de ANA, sacarose, carvão ativado. Não houve resultados satisfatórios para o enraizamento em nenhuma das espécies. Para E. dysenterica, as poucas plântulas que emitiram raízes não suportaram a aclimatização. Para D. alata, não houve emissão de raízes, mas houve emissão de brotações em todos os tratamentos, principalmente naqueles inoculados em meio ½ MS. Para a obtenção dos calos, explantes caulinares de D. alata, e explantes foliares de E. dysenterica, foram inoculados em meio MS, suplementado com diferentes concentrações e combinações de BAP e 2,4 D. Como resultados, obteve-se formação de calos em D. alata com BAP, variando de 0,0 mg.L-1 a 1,0 mg.L-1, interagindo com 2,4 D, variando de 1,0 mg.L-1 e 4,0 mg.L-1. Explantes foliares de E. dysenterica formaram calos entre 3,0 e 4,0 mg.L-1 de 2,4-D combinadas com 0,5 e 1,0 mg.L-1 de BAP.
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Santana, Daniela Maria Andrade. "Micropropagação e etnobotânica de espécies de Bromeliaceae nativas de Sergipe." Pós-Graduação em Desenvolvimento e Meio Ambiente, 2018. http://ri.ufs.br/jspui/handle/riufs/8489.
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Bromeliaceae are characterized as ornamental plants par excellence and are composed of 3,248 species among 58 genera. They are an important taxonomic group, both on the economic scenario - where they impress the ornamental market due to its exotic forms - and in the ecological scenario, in which they are considered amplifiers of the biodiversity. However, Bromeliaceae is currently the second most endangered botanical family. Therefore, the development of ornamental bromeliad cultivation techniques has been considered an important conservation strategy. In the light of the foregoing, the main objective of this research is to apply micropropagation techniques in native Bromeliaceae species with ornamental potential of Sergipe and to carry out their ethnobotanical study, as a form of valorization and conservation. Considering the ecological importance played by the Bromeliaceae family in the Restinga ecosystems, like as their high degree of endemism in these environments, the research was held in a Restinga area located in Aguilhadas community, located in the city of Pirambu, east of Sergipe. From where the mature fruits of Bromeliaceae Aechmea aquilega and Hohenbergia catingae were collected from adult plants in a natural population. The technique used was the propagation through seed germination in vitro, through which three experiments were realized with the bromeliads H. catingae and A. aquilega. The first experiment, in order to analyze the disinfestations of seeds of H. catingae in different times of immersion in sodium hypochlorite. The second experiment evaluated the germination of A. aquilega in medium supplemented culture with the concentrations of 15 g L-¹ and 30 g L-¹ of sucrose. And the third, who investigated the germination of A. aquilega in two levels of maturation of its seeds. The variables analyzed were the Germination Speed Index (IVG) and the Percent Germination. The results showed that the most effective disinfestations was the one with immersion time in the sodium hypochlorite for 20 minutes, segmented in two ten minute periods. For the germination of A. aquilega, it was observed that the culture medium with 15 g L-¹ sucrose is sufficient in germination in vitro. In the third experiment it was verified that the seeds of A. aquilega, in the two stages of maturation, did not differ in the percentage of germination and the IVG. However, in relation to the length of the air component and number of leaves, the treatment that had seeds with less degree of maturation obtained the best results. The etonobotanical study was developed with 20 people from the Aguilhadas community. The sociocultural profile of the interviewees was traced and the knowledge and use of the Bromeliaceae species was analyzed. The approximation of the community by the cultivation of ornamental plants was identified. However, the local population is unaware of the use of bromeliads as ornamental plants. This causes a devaluation of these species, facilitating their extractivism and / or deforestation.As Bromeliáceas são caracterizadas como plantas ornamentais por excelência e são compostas por 3.248 espécies entre 58 gêneros. São um grupo taxonômico importante, tanto no cenário econômico - onde impressionam o mercado ornamental por suas formas exóticas -, quanto no cenário ecológico - no qual são consideradas amplificadoras da biodiversidade. Contudo, a Bromeliaceae é, atualmente, a segunda família botânica mais ameaçada de extinção. Por isso, o desenvolvimento de técnicas de cultivo de bromélias ornamentais têm sido considerado uma importante estratégia para sua conservação. Diante do exposto, a pesquisa teve como objetivo principal aplicar técnicas de micropropagação em espécies de Bromeliaceae com potencial ornamental nativas de Sergipe e realizar um estudo etnobotânico, visando a valorização e conservação. Considerando-se a importância ecológica desempenhada pela família Bromeliaceae nos ecossistemas de Restinga, bem como seu alto grau de endemismo nesses ambientes, a pesquisa foi desenvolvida numa área de restinga situada no povoado Aguilhadas, localizada no município de Pirambu, no leste Sergipano. Foram coletados os frutos maduros das bromeliáceas Aechmea aquilega e Hohenbergia catingae, de plantas adultas em população natural. A técnica utilizada foi a propagação por meio da germinação de sementes in vitro, através da qual realizaram-se três experimentos com as bromélias H. catingae e A. aquilega. O primeiro, a fim de analisar a desinfestação das sementes de H. catingae em diferentes tempos de imersão em solução de hipoclorito de sódio. No segundo experimento foi avaliado a germinação de A. aquilega em MS suplementado com as concentrações de 15 g L-¹ e 30 g L-¹ de sacarose. No terceiro foi investigado a germinação de A. aquilega em dois níveis de maturação das sementes. As variáveis analisadas foram o Índice de Velocidade de Germinação (IVG) e o Percentual de Germinação. Os resultados demonstraram que a desinfestação mais eficaz foi aquela com tempo de imersão em solução de hipoclorito de sódio por 20 minutos, segmentado em dois tempos de dez minutos. Para a germinação de A. aquilega, houve maior germinação no tratamento suplementado com 15 g L-¹ de sacarose ao meio de cultura. No terceiro experimento constatou-se que as sementes de A. aquilega, independente do estágio de maturação, não houveram diferenças na porcentagem de germinação e no IVG. Na fase pós emergência, foram obtidos maiores valores para as variáveis comprimento da parte aérea e número de folhas, no tratamento com sementes que apresentavam menor grau de maturação. O estudo etnobotânico foi desenvolvido com 20 pessoas da comunidade de Aguilhadas, povoado pertencente ao município de Pirambu/SE. Foi traçado o perfil sociocultural dos entrevistados e analisado o conhecimento e uso das espécies de bromeliáceas. Identificou-se a aproximação da comunidade pelo cultivo de plantas ornamentais. Mas a população local desconhece o uso das bromeliáceas como plantas de valor ornamental, o que leva à desvalorização dessas espécies, facilitando seu extrativismo e/ou desmatamento.
São Cristóvão, SE
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Silva, Rayanne Farias da. "Histological analysis of tissues cultured in vitro laticÃferas plants, soluble protein profile and action against plant pathogens." Universidade Federal do CearÃ, 2015. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14733.
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CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel SuperiorLaticifers plants have been studied by presenting a wide range of proteins related to plant defense in its latex. The aim of this study was to investigate, in tissue cultured in vitro, proteins and activities described for latex laticÃferas two species. Tissue callus and roots of Cryptostegia grandiflora were obtained by in vitro tissues culture protocols and subjected to histological analysis for laticifers characterization. Cultured tissue of Calotropis procera were used as comparative reference in the analysis. There wasnât any laticifer structure in callus or roots of C. grandiflora while in C. procera laticifers are formed in the roots. Soluble proteins were extracted from the cultured tissue and characterized using enzymatic assays, biochemical, immunological techniques and mass spectrometry. The presence of activity against phytopathogenic fungi was investigated and all data obtained were compared with the previously one determined for the plants studied latex. Callus and roots proteins of C. procera showed antifungal activity against pathogenic fungi. The percentage inhibition of the vegetative hyphae growth in the presence of callus and roots C. procera protein respectively were 75.5% and 82.6% for Fusarium solani, 76.7% and 57.1% for Rhizoctonia solani, 88.8% and 79.8% for Fusarium oxysporum, 93.7% and 90.2% for Colletotrichum lindemuthianum and 80.2% and 79.7% for Colletotrichum gloesporioides, however, showed no effect on Mucor sp. Callus and roots proteins of C. grandiflora showed no inhibitory effect on the hyphae growth or spores germination of assayed fungi. Through assays using fluorescent markers, it was demonstrated that proteins extracted from in vitro culture of C. procera interact with the membrane of C. gloesporioides causing leakage of cytoplasmic contents, possibly suggesting that its mechanism of action against fungi is related to the change in plasma membrane permeability. Also oxidative stress was observed in C. gloesporioides spores treated with callus and roots protein C. procera by hydrogen peroxide production. Protease inhibitors, chitinases, osmotins and proteases were detected in the C. procera callus and roots samples, however, osmotins and proteases were not observed in C. grandiflora callus and roots. The activity of antioxidant enzymes APX, G-POD and catalase were observed in tissue cultured in vitro of C. grandiflora. Considering that C. grandiflora laticifers proteases were demonstrated exert action against fungi, the results observed in this study suggest that the absence of antifungal activity in C. grandiflora cultured tissue is due to the absence of proteases in these tissues as well exclude chitinases and proteases inhibitors as antifungal proteins. The study concludes that the use of cultured tissues that do not differentiate laticifers is an interesting model to study activities associated to proteins founded in latex. Antifungal proteases present in C. grandiflora latex were not found in the tissues without laticifer formation.
Plantas laticÃferas tÃm sido estudadas por apresentarem uma grande diversidade de proteÃnas relacionadas à defesa vegetal em seu lÃtex. O objetivo deste trabalho foi pesquisar em tecidos cultivados in vitro, proteÃnas e atividades descritas para o lÃtex de duas espÃcies laticÃferas. Tecidos de calos e raÃzes de Cryptostegia grandiflora foram obtidos atravÃs de protocolos de cultura in vitro de tecidos e submetidos à anÃlise histolÃgica para caracterizaÃÃo de laticÃferos. Tecidos cultivados de Calotropis procera foram utilizados como referencial comparativo nas anÃlises. NÃo foi detectada qualquer estrutura laticÃfera em calos ou raÃzes de C. grandiflora enquanto que em C. procera laticÃferos se formam nas raÃzes. ProteÃnas solÃveis foram extraÃdas dos tecidos cultivados e caracterizadas por meio de ensaios enzimÃticos, tÃcnicas bioquÃmicas, imunolÃgicas e espectrometria de massas. A presenÃa de atividade contra fungos fitopatogÃnicos foi investigada e todos os dados obtidos foram comparados com dados previamente determinados para os lÃtex das espÃcies estudadas. ProteÃnas dos calos e raÃzes de C. procera, apresentaram atividade antifÃngica sobre fungos fitopatogÃnicos. Os percentuais de inibiÃÃo do crescimento vegetativo de hifas na presenÃa de proteÃnas de calos e raÃzes de C. procera, respectivamente, foram: 75,5% e 82,6% para Fusarium solani, 76,7% e 57,1% para Rhizoctonia solani, 88,8% e 79,8% para Fusarium oxysporum, 93,7% e 90,2% para Colletotrichum lindemuthianum e 80,2% e 79,7% para Colletotrichum gloesporioides, no entanto, nÃo demonstraram nenhum efeito sobre Mucor sp. As proteÃnas de calos e raÃzes de C. grandiflora nÃo apresentaram qualquer efeito inibitÃrio sobre o crescimento de hifas ou germinaÃÃo de esporos dos fungos avaliados. Por meio de ensaios com marcadores de fluorescÃncia, foi possÃvel demonstrar que as proteÃnas extraÃdas da cultura in vitro de C. procera interagem com a membrana de C. gloesporioides causando extravasamento do conteÃdo citoplasmÃtico, sugerindo que possivelmente seu mecanismo de aÃÃo contra fungos esteja relacionado à alteraÃÃo na permeabilidade da membrana plasmÃtica. TambÃm foi observado estresse oxidativo em esporos de C. gloesporioides tratados com proteÃnas de calos e raÃzes de C. procera atravÃs da produÃÃo de perÃxido de hidrogÃnio. Inibidores de proteases, quitinases, osmotinas e proteases foram detectados nas amostras de calos e raÃzes de C. procera, porÃm, osmotinas e proteases nÃo foram observadas em calos e raÃzes de C. grandiflora. A atividade das enzimas antioxidantes APX, G-POD e catalase foram observadas nos tecidos cultivados in vitro de C. grandiflora. Considerando que proteases laticÃferas de C. grandiflora foram demonstradas exercer aÃÃo contra fungos, os resultados observados nesta pesquisa sugerem que a ausÃncia de atividade antifÃngica em tecidos cultivados de C. grandiflora deve-se a ausÃncia de proteases nestes tecidos e ainda excluem quitinases e inibidores de proteases presentes como proteÃnas antifÃngicas. Em calos e raÃzes de C. procera, alÃm de proteases, outras proteÃnas tais como, quitinases, inibidores de proteases e osmotinas detectadas podem estar envolvidas na atividade antifÃngica observada, e/ou agir sinergicamente na defesa contra fungos. O estudo conclui que o uso de tecidos cultivados que nÃo diferenciam laticÃferos à um interessante modelo para estudar atividades associadas Ãs proteÃnas encontradas no lÃtex. Proteases antifÃngicas presentes no lÃtex de C. grandiflora nÃo foram encontradas nos tecidos sem formaÃÃo laticÃfera.
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Wardrop, Julie. "Biotechnological applications of perfluorochemical liquids in plant tissue culture." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389475.
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Taeb, Abdulkarim Giumaa. "Influence of culture environment on tulip micropropagation." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328788.
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Khalid, Norzulaani. "Somaclonal variation through tissue culture studies in Chrysanthemum morifolium." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329847.
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Sinha, Debleena. "Development of an In Vitro Protoplast Culture System for Albizia Lebek (L.) Benth., an Economically Important Leguminous Tree." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc500422/.
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An in vitro system of generating protoplasts from their callus cultures was established. The friable callus was more productive in terms of producing protoplasts than the green compact callus. The concentration of the various cell wall degrading enzymes had an effect on the viability of the protoplasts in the medium. The protoplast system developed from the experiments was stable and could be used for the transformation experiments of Albizia lebek and for other plant improvement practices.
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Fei, Liwen. "Towards automating micropropagation: from cells to shoots to plants in one step." Digital WPI, 2015. https://digitalcommons.wpi.edu/etd-dissertations/195.
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A mist reactor was used to study plant growth and development under various environmental conditions towards the production of healthy plantlets ready for soil transplant in one step from inoculation. In addition, a 3D type of cultivation via surface attachment of explants to vertically hanging strips inside the mist reactor was also investigated to maximize productivity with minimal footprint. Using carrot as the model species, pre-embryogenic cell suspensions were successfully spray-inoculated onto hanging poly-L-lysine (PLL)-coated nylon mesh to which they then attached and remained for several weeks while they developed into rooted plantlets. To study single step micropropagation from shoot explants to fully acclimatized plantlets, Artemisia annua was used as the model species. Nodal cuttings of A. annua were inoculated onto PLL-coated mesh strips by briefly immersing the strips in the suspension of nodal cuttings. Investigation of medium, phytohormones, CO2, ventilation level and humidity ensued resulting in selection of a preferred final process that reduced physiological aberrations like hyperhydricity and was time efficient. The nodal cuttings that attached to the strips were first misted with half strength shooting medium for 7 days to develop new shoots. Then the new shoots were misted with the rooting medium supplemented with NAA for 12 days to develop roots. Rooted plantlets were acclimatized in the same rooting medium for 9 days to acquire fully functional stomata prior to planting into soil. Taken together this study suggested that fully developed plantlets ready for planting into soil could be obtained in a single step in a bioreactor from embryogenic cells or from nodal explants.
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Mlungwana, Asanda. "In-vitro propagation studies of the endangered succulents Drosanthemum Micans and Drosanthemum Hallii (Aizoaceae)." Thesis, Cape Peninsula University of Technology, 2018. http://hdl.handle.net/20.500.11838/2748.
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Thesis (MTech (Horticulture))--Cape Peninsula University of Technology, 2018.Drosanthemum micans and Drosanthemum hallii are endangered succulent shrubs of horticultural and medicinal value. They are restricted to the Succulent Karroo, which is one of the world’s biodiversity hotspots. The species risk extinction from illegal over-harvesting for water-wise gardens, erosion by occasional flush floods from ephemeral rivers, competition from alien invasive species, overgrazing and clearing of land for agriculture and human settlement. Although seeds and cuttings may be used in propagating these species, they often require seasonal collection and planting and cuttings struggle to establish, hence the need for in-vitro propagation as an alternative solution. Thus, the main objective of the study was to develop a method for rapid in-vitro shoot and root multiplication and acclimatization of D. micans and D. hallii. To initiate shoot formation, disinfected leaf and stem nodal explants were cultured in Murashige and Skoog (1962) media supplemented with different rates (0, 10, 20 or 30μM) of 2-isopentyladenine, 6-Benzyladenine and kinetin for D. hallii or 2-isopentyladenine, 6-Benzyladenine and Thiadiazuron for D. micans. Shoots from explants were rooted in varying rates (0, 10, 20 or 30μM) of IAA for root initiation. Three media, which were used in previous studies, were tested for acclimatization of rooted explants in i) vermiculite, ii) sand (50%): vermiculite (50%) or iii) sand (75%): perlite (25%). For quantitative evaluation of plant stress, chlorophyll fluorescence index (Fv/Fm) was measured as a proxy for plant stressf stress. It emerged that stem nodal explants of D. hallii tend to produce multiple shoots whilst leaf explants tended to produce callus when cultured in full-strength Murashige and Skoog (1962). Shoot multiplication was optimal in both D. hallii and D. micans at 10 μM of kinetin. Root formation in both D. hallii and D. micans only occurred when shoots were transferred to a full-strength Murashige and Skoog (1962) media without any phytohormones added. The intensity of tissue browning increased at higher levels of cytokinins, suggesting an interaction of plant growth regulators with exudates from explants. Different acclimatization media tested showed no significant differences in the level of stress (Fv/Fm). It is recommended that Murashige and Skoog (1962) media with10 μM kinetin be used for shoot development and multiplication, followed by transfer of the shoots to fresh full-strength Murashige and Skoog (1962) media without hormones for root development. Acclimatization of the rooted explants was possible in one of the following media: i) vermiculite, ii) sand (50%): vermiculite (50%) or iii) sand (75%): perlite (25%) and in a misted greenhouse (ca. 60% RH), with gradual weekly reductions in humidity by 10% over 2 weeks.
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Al, Kaabi Helel Humaid Saed Humaid. "Date palm tissue culture and AFLP analysis of plant variability." Thesis, Imperial College London, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409314.
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Ghadimzadeh, Mortaza. "Studies of protoplast and liposome techniques in plant tissue culture." Thesis, University of Salford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.255239.
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Lappin, G. J. "The biotransformation of monoterpenoids by plant cells in axenic culture." Thesis, University of Westminster, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382797.
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Heyenga, Gerard. "Tissue culture of Podophyllum hexandrum and production of anticancer ligands." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235985.
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Redway, F. A. "Regeneration in tissue and protoplast culture of Sainpaulia ionantha (African violet)." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382673.
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Watson, L. D. "Induction and assessment of plant cell membrane permeability." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233331.
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This thesis describes the isolation, immobilisation and permeabilisation of Digitalis lanata (foxglove) and Nicotiana tabacum (tobacco) plant cells and protoplasts. Protoplasts were isolated from leaves of Digitalis lanata and Nicotiana tabacum and cultured in conditions of varying osmotic potential, illumination and cell density in order to achieve maximum cell stability. The regeneration of cellulose cell walls in Nicotiana tabacum protoplasts could be inhibited by the addition of dilute concentrations of the herbicide 2,6-Dichlorobenzonitrile. At concentrations of 2-10 mg/l in culture medium cell wall regeneration could be prevented for up to 6-8 weeks. Isolated cells and protoplasts were stabilised by entrapment within a beaded agarose support matrix. Nicotiana tabacum protoplasts and cells, immobilised within an agarose beaded support matrix were used to develop a technique to permeabilise and destabilise the cell membranes. Cells or protoplasts pre-loaded with 6-Carboxy-Fluorescein, Neutral Red stains and 14C-Sucrose or 86Rb+ radioactive tracers were employed as markers for cell permeability or leakiness. Efflux or Compartmental analysis was used to determine the influence of various selected permeabilising agents on the integrity and the leakiness of either protoplast or cell membranes. Immobilised protoplasts or cells were subjected to a three - step procedure involving initial loading with 86Rb+ tracer, a membrane permeabilisation step and finally a recovery step. Protoplast membrane stability could not be regained after the recovery step, following a 30 minute period of permeabilisation with 50 mM acetic acid in culture medium. Immobilised cells could, however, regain membrane integrity with good intracellular retention of 86Rb+ tracer ion during the efflux experiment. Thus, immobilised Nicotiana tabacum cells could be made reversibly permeable with the use of specific agents. It is anticipated that such techniques which encourage reversible permeabilisation of immobilised cells have potential in larger scale plant cell culture systems to effect the release of intracellularly stored, useful secondary metabolites. There are also possibilities for the inclusion of exogenous precursors, cofactors and foreign genetic material which would increase compound yield and may produce novel secondary metabolites.
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Blakemore, Philip Alexander. "Optimisation of steam reconditioning for regrowth-ash and plantation-grown eucalypt species." Connect to full text, 2008. http://ses.library.usyd.edu.au/handle/2123/2343.
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Thesis (Ph. D.)--University of Sydney, 2008.Includes graphs and tables. Includes list of publications: p. iv. Title from title screen (viewed May 5, 2008). Thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the School of Chemical and Biomolecular Engineering. Includes bibliographical references. Also available in print form.
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THOMAS, JOHN CALVIN. "THE CONSEQUENCES OF BROMODEOXYURIDINE TREATMENT IN PLANT TISSUE CULTURES (REGENERATION, REPLICATION)." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183989.
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Plant tissue culture regeneration is chiefly regulated by exogenous phytohormones. To stop regeneration and induce undifferentiated callus growth auxins are used. Unfortunately auxins influence many plant responses, most unrelated to development. Using the thymidine analogue 5-bromodeoxyuridine (BrdU) a phytohormone independent means for differentiation inhibition has been developed. Studies were focused on the target site and mechanism of BrdU action. The BrdU inhibited step in development is indicative of a plant response necessary for normal differentiation. BrdU (5-30 uM) interrupts callus growth in all tested plants. Exogenous cytokinin does not restore growth while thymidine and deoxycytidine rescue plant growth and differentiation in the presence of BrdU. Endogenous cytokinin levels are not greatly affected by subtoxic BrdU levels and indicate that cytokinin and BrdU act upon independent sites. Domestic carrot cells were used in further studies. Normally carrot cells are undifferentiated in medium with the auxin 2,4D. When 2,4D is removed, somatic embryogenesis takes place. By including 5 uM BrdU in the hormoneless medium, the cells fail to differentiate. The growth of carrots in 2,4D is not affected by 5 uM BrdU. Thus, BrdU influences growth during differentiation to a greater extent than the growth of callus cells. BrdU is effective in halting development when applied 0-24 hours after differentiation induction. An event required for differentiation (the first and second replications) must take place at this time. BrdU action begins with DNA incorporation. The consequent cellular replication becomes slowed and DNA repair results. At the same time RNA and protein levels are similar in BrdU treated and untreated cultures. BrdU thymidine substitution into DNA increases from 28% (2 days) to 68% (3 days) after embryogenic induction. A second BrdU effect follows DNA incorporation. Factors (MIFs) in the medium of BrdU treated cells arrest differentiation. After BrdU is repaired from the DNA, the cells are only able to differentiate after a medium change. Understanding MIF production could explain why some plants differentiate more readily than others. BrdU provides the means for further study of MIF's in the auxin-free inhibition of development.
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Kaparakis, Georgios. "In vitro culture of pepper (Capsicum annuum L.)." Thesis, University of Nottingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297989.
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Holden, Peter Richard. "Variability in cultured cells of Capsicum Spp." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/10960.
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Borda, Yepez Charlotte Cesty 1976. "Potencial morfogenético e carotenóides em tecidos cultivados in vitro de Pothomorphe umbellata L. /." Botucatu : [s.n.], 2003. http://hdl.handle.net/11449/103306.
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Orientador: Giuseppina Pace Pereira LimaBanca: Eny Iochevet Segal Floh
Banca: João Domingos Rodrigues
Resumo: O presente trabalho teve como objetivo estabelecer o protocolo de desinfestação de estacas e sernentes e germinação de Pothomorphe umbeilata (L) e adaptar o protocolo de micropropagação de Pothomorphe umbeilata (L), incluindo a produção de calos e organogênese, além de comparar o teor de carotenóides entre calos e plântulas. O experimento foi conduzido no Laboratório de Biotecnologia Vegetal do Departamento de Química e Bioquímica, do Instituto de Biociências da UNESP - Botucatu, SP e o material vegetal (sementes e estacas) foi obtido no município de Adrianópolis-PR. Semente germinadas de P. umbeilata foram inoculadas em diferentes concentrações de BAP (0,5 mg.L; 1,0 mgL; 1,5 mg.L) e NAA (0,4 mg.L 0,6 mg.L; 0,6 mg.L) respectivamente, visando estimular a produção de calos e dosar o carotenóides. Após 60 dias do cultivo, os calos contendo algumas brotações, foram transferidos para meio de diferenciação das plântulas (GA3 0,1 mg.L, BAP 0,5 mgL) por um período de 40 dias, para logo serem transferidos para meio de diferenciação de plântulas. Calos (coletados aos 60 dias) e plântulas (coletadas aos 140 dias) foram congelados em nitrogênio líquido e mantidos em freezer a 80°C para posteriores análises de carotenóides. O melhor tratamento para a produção de calos e formação de gemas foi NAA 0.6 mgL em combinação com BAP 10 mg.L. Nas plântulas sem adição de reguladores vegetais foi encontrada uma maior concentração de carotenõides, em comparação aos calos.
Abstract: The present research aimed at establishing a protocol of stalks and seed desinfectation, and seed germination of Pothomorphe umbeilata (L.). A protocol of micropropagation including the induction of callus formation and organogenesis was adapted. Besides, it was compared the carotenoids quantity between Porhomorphe umbeliata calius and plantlets. This experiment was carried out iii Biotechnology Vegetal Laboratory of the Chernistr and Biochemistry Department at the Instituto de Biociências of Universidade Estadual Paulista, Botucatu campus. The vegetal matenal (seed and stalks) was obtained from Adranópolis - PR. Pothomorphe umbeilata germinated seeds were inoculated in different concentrations of BAP (0,5 mg.L-l; 1,0 mg.L.-l; 1,5 mg.L-l) and NAA (0,4 mg.L-l; 0,6 mg.L-1; 0,6 mg.L-l) respectively, in order to stimulate calius induction and increase quantity of earotenoids. Afler 60 days, calius which contained shoots were inoculated in plantlets diferenciation medium GA3 0,1 mg.L4, BAP 0,5 mg.U) during 40 days and transferred to plantlets growth medium. Plantlets were acclimatized Calius collected after 60 days and plantlets were collected afier 140 days. There were frozen in liquid nitrogen and maintained in freezer 80°C to be used in further carotenoids test. The best treatment for callus production and shoots elongation was NAA 0.6 mg.L in association with BAP 1.0 mg.L. The higher carotenoids coneentration was in plantlets without growth regulators, compared with calius.
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Ebida, Aly Ibrahim Aly. "Evaluation of phenotypic stability and salinity tolerance in tissue culture : propagated plants of strawberry (Fragaria x ananassa Duch.) cultivar 'Tioga'." Thesis, Imperial College London, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273270.
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Reinhold, Dawn Marie. "Fate of fluorinated organic pollutants in aquatic plant systems studies with lemnaceae and lemnaceae tissue cultures /." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/26506.
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Thesis (Ph.D)--Civil and Environmental Engineering, Georgia Institute of Technology, 2008.Committee Chair: Saunders, F. Michael; Committee Member: Huang, Ching-Hua; Committee Member: Hughes, Joseph; Committee Member: Loeffler, Frank; Committee Member: Pullman, Gerald; Committee Member: Spain, Jim. Part of the SMARTech Electronic Thesis and Dissertation Collection.
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Hamidoghli, Yousef. "Production and identification of interspecific potato somatic hybrids." Thesis, University of the West of Scotland, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283091.
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Spencer, Andrew. "The development of shooty teratomas in Mentha species by genetic manipulation and studies on their growth and terpene production in vitro." Thesis, University of East Anglia, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292258.
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Gibbs, Margaret Joan. "Genetic engineering of the forage legume Lotus corniculatus using Agrobacterium : mediated transformation systems." Thesis, Durham University, 1991. http://etheses.dur.ac.uk/6040/.
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Gene transfer vectors based on the Agrobacterium tumefaciens Ti plasmid were used to develop a successful disarmed Agrobacterium tumefaciens-mediated transformation method for Lotus comiculatus. A binary vector construct, pJIT73, was used during the development of the Agrobacterium tumefaciens transformation system due to its selectable (Aph IV, nos- neo) and scorable markers. The effects of the antibiotics geneticin (G-418) and hygromycin B were studied. Use of kill curves and selection delay experiments allowed potentially suitable selection pressure parameters to be proposed. Using such selection during transformation experiments led to further optimisation of this stage of transformation. The influence of plant hormones on the regeneration of Lotus comiculatus explants was investigated and a modification of an established protocol using leaf explants was introduced as an attempt to reduce the overall time of regeneration. Various explants were used but leaf pieces were chosen as the most suitable explant on which to focus research. So, through alteration of various stages, including length of cocultivation and subsequent decontamination within the transformation process, a successful method was developed. Experiments indicated the optimum Agrobacterium tumefaciens strain to be used with Lotus comiculatus was the disarmed Ach5 type, LBA4404(pAL4404). Transgenic Lotus comiculatus plants were produced which expressed the scorable marker β-Glucuronidase gene (GUS) and the selectable marker for hygromycin B resistance, AphIV. Gene transfer was confirmed by Southern blotting. The new Agrobacterium tumefaciens-mediated vector system was used to introduce the cowpea trypsin inhibitor gene (CpTi) into Lotus comiculatus. However, although there was evidence for transformed callus development, no shoots were induced. By the use of previously established Agrobacterium rhizogenes-mediated system, an attempt was made to introduce the pea lectin gene (psl) into Lotus corniculatus. Hairy root regenerants were produced but genetic transfer was unconfirmed and attempted investigation of the plant - Rhizobium symbiosis involving Lotus corniculatus was not fulfilled.
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Khatun, Asma. "The genetic manipulation of jute (Corchorus) species." Thesis, University of Nottingham, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335652.
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Smith, Elaine Francis. "The preparation of micropropagated plantlets for transplantation." Thesis, University of East London, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.258546.
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Hudson, G. "Micropropagation and low temperature storage of Dieffenbachia." Thesis, University of East London, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370763.
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Kirby, Nigel John. "An investigation of metabolite release from plant cells in vitro to their surrounding medium." Thesis, University of the West of England, Bristol, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329861.
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Satchwell, Christa Elizabeth. "Investigation of the nutrient requirements of Pinus caribaea Morelet in vitro." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305742.
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Gunasekare, M. T. K. "In vitro culture directed towards plant improvement of tea (Camellia sinensis var. assamica)." Thesis, University of Southampton, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241937.
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Earnshaw, Brent A. "The role of cellular antioxidants (glutathione and ascorbic acid) in the growth and development of wild carrot suspension cultures." Diss., Georgia Institute of Technology, 1986. http://hdl.handle.net/1853/5623.
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Qouta, Lolita Abdulla. "The biochemistry and molecular biology of intercellular adhesion in plant tissue culture." Thesis, University of Glasgow, 2008. http://theses.gla.ac.uk/302/.
Full textAbstract:
The adhesion between neighbouring plant cells is established as cells are formed during cytokinesis through the middle lamella that is made principally of pectins and proteins. Pectins are secreted into the cell wall in a highly methylesterified form and subsequently de-esterified in muro by pectin methyl esterase (PME, E.C. 3.1.11). The present study reports on the biochemical characterization and immunochemical analyses of phosphate buffer/EDTA pectic extracts associated with cell-cell adhesion in suspension cultures of wild type (WT), salt tolerant (HHS) cell lines and synchronized Arabidopsis suspension cultures. Using the synchronized cultures, The PME-mediated configuration of pectins at the onset of adhesion during cytokinesis, was assessed through the analysis of the expression patterns of the PME isoforms annotated to be expressed throughout the cell cycle The wild type Arabidopsis seemed to maintain the intercellular adhesion through the gelling of the highly methylated JIM7 recognized homogalacturonans that were shown to be abundant in the primary cell walls, middle lamellae and cellular junctions, possibly due to the hydrophobic interactions between the methoxy groups. The rhamnogalacturonan-I fraction was rich in arabinan side chains reflecting the proliferative state of the cells. The increase in arabinan content was accompanied by a reduction in the galactan content 4 days after subculturing. The cell walls of salt tolerant Arabidopsis contained the JIM7 and LM7recognized epitopes along with a high degree of branching of rhamnogalacturonan-I carrying galactans and arabinans as side chains. The change in the detected epitopes is thought to play a role in the ability of the cells to withstand the high osmotic pressure and increase the in the level of adhesion between cells. The JIM5 low methylesterified HGs were less abundant in both cultures, and the absence of the 2F4 antibody recognizing the Ca2+ egg boxes could be attributed to the scarce amounts of Ca2+ present in the culturing medium The immunochemical studies of the pectin extracted from the synchronized Arabidopsis suspension cultures after washing out aphidicolin indicated that the recognition of both of JIM7 and JIM5 varied in parallel during the cell cycle, whereas, the recognition of arabinan increased during the cell division. The sequence and phylogenetic analysis of ten PME isoforms that were annotated to be expressed at one or more phases of the cell cycle of synchronized Arabidopsis thaliana suspension cultures (Menges and Murray, 2002 and 2003), revealed that only five of these genes could be PMEs. The genes At4g02330, At1g02810, At2g26440, and At2g47550 were thought to be of type II PMEs which have a pre-pro-catalytic domains and At5g47500 is a type I PME that lack the pro-region. The amino acid sequence of At4g12390 showed similarities with the N-terminal pro-peptides of plant PME and invertase inhibitors. The expression of several PME genes was studied in suspension cultures of Arabidopsis thaliana synchronised using aphidicolin. Semi-quantitative PCR experiments showed that the expression of At5g47500 transcript was always detected during M phase of the cell cycle. The rest of the genes failed to show consistent patterns of expression. Northern blots revealed that mRNA coding for At5g47500 decreases during S and G2 phases and accumulates during the M phase of the cell cycle. Our results suggest that this PME isoform is involved in the modulations of the cell walls as the cells are going through division and cytokinesis.