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Journal articles on the topic 'Tissue culture in plants'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 February 2022

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1

Niazian, M., S. A. Sadat Noori, P. Galuszka, and S. M. M. Mortazavian. "Tissue culture-based Agrobacterium-mediated and in planta transformation methods." Czech Journal of Genetics and Plant Breeding 53, No. 4 (2017): 133–43. http://dx.doi.org/10.17221/177/2016-cjgpb.

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Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method. The main problem of Agrobacterium-method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. Development of genotype-independent gene transformation method is of great interest in many plants. Some tissue culture-independent Agrobacterium-mediated gene transformation methods

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2

van der Linde, P. C. G. "CERTIFIED PLANTS FROM TISSUE CULTURE." Acta Horticulturae, no. 530 (September 2000): 93–102. http://dx.doi.org/10.17660/actahortic.2000.530.9.

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3

Lee, Michael, and R. L. Phillips. "Genomic rearrangements in maize induced by tissue culture." Genome 29, no. 1 (1987): 122–28. http://dx.doi.org/10.1139/g87-021.

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Chromosomal instability is a common occurrence in plant tissue cultures and has been documented in plants regenerated from several genotypes of maize (Zea mays L.) tissue cultures. The objective of this research was to evaluate the frequency and types of chromosomal aberrations in regenerated plants of an Oh43–A188 genetic background, which had not been examined previously for chromosome stability in culture. Organogenic callus cultures were intitated from immature embryos of F2 plants for several Oh43 ms isoline × A188 crosses. The chromosome constitution of 267 plants was investigated throug

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4

Seabrook, Janet E. A., and Gerald Farrell. "City Water Can Contaminate Tissue Culture Stock Plants." HortScience 28, no. 6 (1993): 628–29. http://dx.doi.org/10.21273/hortsci.28.6.628.

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Stock plants of `Shepody' and `Yukon Gold' potato (Solarium tuberosum L.) were grown in a greenhouse and irrigated with city water. Contamination rate of stem explant tissue cultures excised from these stock plants was 50% to 100%. A comparison of the microorganisms isolated from the contaminated cultures and from 0.22-μm filter disks through which 20 liters of city water had passed revealed the presence of similar bacterial floras. Five genera of bacteria (Listerium spp., Corynebacterium spp., Enterobacter spp., Pasteurella spp., and Actinobacillus spp.) were isolated from contaminated cultur

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5

Johnson, K. "TISSUE CULTURE OF AUSTRALIAN PLANTS - A REVIEW." Acta Horticulturae, no. 447 (October 1997): 515–28. http://dx.doi.org/10.17660/actahortic.1997.447.102.

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6

Kunakh, V. A., D. O. Navrotska, M. O. Twardovska, and I. O. Andreev. "Peculiarities of chromosomal variability in cultured tissues of Deschampsia antarctica Desv. plants with different chromosome numbers." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 14, no. 1 (2016): 36–43. http://dx.doi.org/10.7124/visnyk.utgis.14.1.542.

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Aim. To clarify the details of chromosome variation in calli derived from D. antarctica plants in the initial passages of the culture in vitro. Methods. Induction of callus from root explants of plants, which were grown from seeds, and consequent subcultivation of tissue culture. Cytogenetic analysis of squashed slides stained by acetic-orcein and counting the number of chromosomes in mitotic metaphase plates. Results. There were analyzed the cultured tissues derived from D. antarctica plants with different chromosome numbers: diploid plants (2n=26), mixoploid plant with B-chromosomes (2n=26+1

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7

Twardovska, M. O. "THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE." Biotechnologia Acta 14, no. 2 (2021): 59–66. http://dx.doi.org/10.15407/biotech14.02.059.

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Aim. The aim of the study was to determine the quantitative and qualitative content of phenolic compounds and flavonoids in Deschampsia antarctica E. Desv. tissue cultures obtained from plants originating from different islands of the maritime Antarctic. Methods. In vitro tissue culture, Folin-Ciocalteu method, spectrophotometry, HPLC analysis. Results. The quantitative content of phenolic compounds and flavonoids in D. antarctica tissue cultures obtained from plants of six genotypes (DAR12, DAR13, G/D12-2a, Y66, R30 and L57) was determined. The highest content of phenolic compounds (4.46 and

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8

Ruan, Yiqin, and Mark H. Brand. "In Vitro Responses of Tissues from Rhododendron Plants With and Without Tissue Proliferation." HortScience 30, no. 4 (1995): 873D—873. http://dx.doi.org/10.21273/hortsci.30.4.873d.

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Rhododendron `Montego' shoot cultures initiated from plants with and without tissue proliferation (TP and NTP) served as explant sources for all studies (Note: in vitro TP shoot cultures produce primarily dwarf shoots, some long shoots, and stem tumors). Calli induced from TP leaves and tumors and NTP leaves were cultured on woody plant (WP) medium containing NAA and 2-iP. During the first 4 weeks of culture, calli from NTP leaves had higher relative growth rates than calli from TP leaves or tumors. However, calli from TP leaves and tumors grew faster than calli from NTP leaves for all subcult

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9

panigrahi, Sunitha, Dr k. Aruna Lakshmi, and Nida Mir. "Micro Propagation and Plant Strengthening of Tissue Cultured Plants, Inoculated with Several Bacterial Strains." International Journal of Scientific Research 2, no. 8 (2012): 15–17. http://dx.doi.org/10.15373/22778179/aug2013/7.

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10

Baillie, A. M. R., K. K. Kartha, and B. G. Rossnagel. "Evaluation of 10 Canadian barley (Hordeum vulgare L.) cultivars for tissue culture response." Canadian Journal of Plant Science 73, no. 1 (1993): 171–74. http://dx.doi.org/10.4141/cjps93-023.

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Ten Canadian barley cultivars — Abee, Deuce, Ellice, Harrington, Manley, Bonanza, Conquest, Duke, Heartland, and Samson — were evaluated for tissue-culture response. Callus was obtained from embryos 3–5 d post anthesis from all cultivars. Fertile plants were regenerated from eight. Abee cultures gave the best response in terms of the number of plants regenerated, while Bonanza and Samson cultures produced no regenerated plants. Heartland and Deuce were selected for further study to determine optimum growth-regulator concentrations for callus production and plant regeneration. Two growth regula

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11

Rhodes, C. A., R. L. Phillips, and C. E. Green. "Cytogenetic stability of aneuploid maize tissue cultures." Canadian Journal of Genetics and Cytology 28, no. 3 (1986): 374–84. http://dx.doi.org/10.1139/g86-055.

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Monosomic maize tissue cultures might be used to select recessive mutations of cellular traits. This strategy would avoid some of the problems encountered with haploid cultures such as lack of vigor, sterility of regenerated plants, and uncontrolled diploidization. Monosomic and other aneuploid plants were selected among progeny of W22 R/r-x1 crossed with genetic stocks containing recessive markers. The r-x1 allele induces aneuploidy at a frequency of about 15%. Immature tassels of selected plants were used to initiate totipotent tissue cultures. Plants were regenerated from the cultures over

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12

Lee, Michael, and R. L. Phillips. "Genetic variants in progeny of regenerated maize plants." Genome 29, no. 6 (1987): 834–38. http://dx.doi.org/10.1139/g87-142.

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Tissue culture has been shown to be a method of generating genetic variation in regenerated plants and their progeny for several maize (Zea mays L.) genotypes. The objectives of this study were to (i) estimate the frequency and types of variants arising from maize tissue cultures, (ii) investigate the effect of culture age on the frequency of variants per regenerated plant, and (iii) estimate the frequency of sectoring among regenerated plants of an F3 from Oh43/A188 genetic background that had not been examined previously for genetic stability in culture. Organogenic callus cultures were init

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13

Briggs, B. "COMMERCIAL PRODUCTION OF ERICACEOUS PLANTS BY TISSUE CULTURE." Acta Horticulturae, no. 212 (September 1987): 644. http://dx.doi.org/10.17660/actahortic.1987.212.107.

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14

Zimmerman, R. H. "MICROPROPAGATION OF WOODY PLANTS: POST TISSUE CULTURE ASPECTS." Acta Horticulturae, no. 227 (September 1988): 489–99. http://dx.doi.org/10.17660/actahortic.1988.227.102.

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15

Fay, Michael F., Jill Gratton, and Peter J. Atkinson. "Tissue culture of succulent plants - An annotated bibliography." Bradleya 13, no. 13 (1995): 38–42. http://dx.doi.org/10.25223/brad.n13.1995.a6.

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16

Florkowski, Wojciech J., Orville M. Lindstrom, Carol D. Robacker, and H. R. Simonton. "Analysis of Pricing Plants Grown in Tissue Culture." HortScience 25, no. 10 (1990): 1306. http://dx.doi.org/10.21273/hortsci.25.10.1306.

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17

SAITO, Kazuki. "Genetic Engineering in Tissue Culture of Medicinal Plants." Plant tissue culture letters 10, no. 1 (1993): 1–8. http://dx.doi.org/10.5511/plantbiotechnology1984.10.1.

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18

Scherwinski-Pereira, J. E., F. H. S. Costa, J. Camillo, D. B. Silva, R. B. N. Alves, and R. F. Vieira. "TISSUE CULTURE STORAGE OF BRAZILIAN MEDICINAL PLANTS GERMPLASM." Acta Horticulturae, no. 860 (February 2010): 211–14. http://dx.doi.org/10.17660/actahortic.2010.860.31.

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19

TOYODA, Hideyoshi. "Selection of disease resistant plants through tissue culture." Kagaku To Seibutsu 28, no. 1 (1990): 12–19. http://dx.doi.org/10.1271/kagakutoseibutsu1962.28.12.

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20

Stephens, P. A., C. D. Nickell, and J. M. Widholm. "Agronomic evaluation of tissue-culture-derived soybean plants." Theoretical and Applied Genetics 82, no. 5 (1991): 633–35. http://dx.doi.org/10.1007/bf00226802.

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21

von Aderkas, P., and J. M. Bonga. "Plants from haploid tissue culture of Lavix decidua." Theoretical and Applied Genetics 87, no. 1-2 (1993): 225–28. http://dx.doi.org/10.1007/bf00223768.

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22

Müller, E., P. T. H. Brown, S. Hartke, and H. Lörz. "DNA variation in tissue-culture-derived rice plants." Theoretical and Applied Genetics 80, no. 5 (1990): 673–79. http://dx.doi.org/10.1007/bf00224228.

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23

Benzion, Gary, and Ronald L. Phillips. "Cytogenetic stability of maize tissue cultures: a cell line pedigree analysis." Genome 30, no. 3 (1988): 318–25. http://dx.doi.org/10.1139/g88-056.

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Cytogenetic instability in maize plants regenerated from tissue culture is a commonly observed phenomenon. In an attempt to understand the origin of this instability meiotic analyses were performed on 370 regenerated plants that were initiated from cell lines of 22 immature embryos of 9 maize genotypes. Cell lineage pedigrees were maintained on these cultures to record the familial relationship between regenerated plants. Overall, 12.4% of the 370 régénérants contained cytological aberrations. The largest category of aberrations involved chromosome breakage (translocations, deletions, and an i

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24

Wang, Hui, Guanyong Luo, Jiayuan Wang, et al. "Flavonoids Produced by Tissue Culture of Dracaena cambodiana." Natural Product Communications 9, no. 1 (2014): 1934578X1400900. http://dx.doi.org/10.1177/1934578x1400900113.

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Dragon's blood is a traditional medicine used in many countries of many cultures because of its various therapeutic uses, and its main bioactive compounds are flavonoids, which mainly exhibit antitumor and antimicrobial activities. In the process of tissue culture of Dracaena cambodiana, one of its resource plants, red secretion was discovered in the culture when 6-benzylaminopurine was added. Analysis of its constituents by HPLC in comparison with dragon's blood and the standards proved that 17 compounds, including 10 flavonoids, are the same as those in dragon's blood. It is promising that f

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25

Cullis, C. A., and W. Cleary. "DNA variation in flax tissue culture." Canadian Journal of Genetics and Cytology 28, no. 2 (1986): 247–51. http://dx.doi.org/10.1139/g86-034.

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The DNAs from leaves and callus from a series of flax genotrophs have been compared. The probes used for this comparison represent all of the highly repeated DNA sequence families in the flax genome. The abundance of most of the families could vary in culture, but the extent of variation was dependent on the genotroph. The extent of the variation observed between leaf DNA and callus DNA from a single genotroph was greater than that observed between the genotrophs in vivo. The DNAs from the progeny of a number of regenerated plants were also compared. They sometimes differed both from the callu

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26

Thomas, J. "Commercial Plant Tissue Culture in India—Current Status." HortScience 30, no. 4 (1995): 757B—757. http://dx.doi.org/10.21273/hortsci.30.4.757b.

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In recent years, there has been an explosion in the number of commercial plant tissue culture (TC) units in India. More than 25 such companies have production capacity of two to five million plants per annum. Almost all units are export oriented, but the target crops are the same. Indoor foliage plants dominate the export market. Micropropagation industry in India is providing major support to Indian agriculture in four crop groups: Fruits, ornamentals, spices, forestry/plantation crops. Banana is the largest selling TC fruit crop. TC papaya plants are now marketed for extraction and processin

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27

Dogsom, Bolormaa, and Buyanchimeg Batsukh. "Tissue culture of the monocot." Mongolian Journal of Agricultural Sciences 11, no. 2 (2014): 87–91. http://dx.doi.org/10.5564/mjas.v11i2.225.

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PsbS is a 22-kDa protein of photosystem II involved in nonphotochemical quenching of chlorophyll fluorescence but the mechanism is still unclear. We designed and generated transgenic rice plants with significantly reduced PsbS1 protein level using RNA interference (RNAi). Transformation confirmed by vector-specific primers and transformants were screened by RT-PCR for OsPsbS1 transcript levels and PsbS1 protein level. We could identify three PsbS1-RNAi lines. DOI: http://dx.doi.org/10.5564/mjas.v11i2.225 Mongolian Journal of Agricultural Sciences Vol.11(2) 2013 pp.87-91

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28

Wightman, Raymond, and C. J. Luo. "From mammalian tissue engineering to 3D plant cell culture." Biochemist 38, no. 4 (2016): 32–35. http://dx.doi.org/10.1042/bio03804032.

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Recent advances applying mammalian tissue engineering to in vitro plant cell culture have successfully cultured single plant cells in a 3D microstructure, leading to the discovery of plant cell behaviours that were previously not envisaged. Animal and plant cells share a number of properties that rely on a hierarchical microenvironment for creating complex tissues. Both mammalian tissue engineering and 3D plant culture employ tailored scaffolds that alter a cell's behaviour from the initial culture used for seeding. For humans, these techniques are revolutionizing healthcare strategies, partic

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29

Sultanbawa, Fazal, Sharad C. Phatak, and Casimir A. Jaworski. "TISSUE CULTURE OF CUPHEA GLUTINOSA CHAM. & SCHLECHT." HortScience 25, no. 9 (1990): 1137e—1137. http://dx.doi.org/10.21273/hortsci.25.9.1137e.

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Caphea glutinosa is a herbaceous, low-growing annual, bearing numerous attractive purple flowers and has potential as an ornamental and as a ground cover. Plants exhibit winter hardiness in USDA plant hardiness zone 8. Tissue culture techniques were developed to obtain large numbers of uniform plants. Whole leaf explants (approximately 1.0 cm2) callused profusely in MS (Murashige and Skoog, 1962) medium containing 84 mM sucrose, 1% (w/v) Difco Bacto agar and 8.8 μM N6benzyladenine. Shoot formation from calli was observed in the same medium 4 weeks after explanting. Detached shoots were rooted

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30

Tisserat, Brent. "Establishing Tissue-cultured Sweetgum Plants in Soil." HortTechnology 15, no. 2 (2005): 308–12. http://dx.doi.org/10.21273/horttech.15.2.0308.

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Methods to enhance sweetgum (Liquidambar styraciflua L.) in vitro axillary shoot formation and shoot establishment into soil are presented. Sweetgum shoots grown in an automated plant culture system (APCS) produced 400 to 500 shoots via axillary branching compared to only 40 shoots produced within Magenta vessels containing agar medium after 8 weeks of incubation. Vitrification was observed in as many as 80% of the axillary shoots produced in the APCS. A continuous carbon dioxide (CO2)-flow-through system was tested on both vitrified and non-vitrified sweetgum shoots transferred from the APCS

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31

AboEl-Nil, M. M. "TISSUE CULTURE OF NATIVE PLANTS IN THE DEVELOPING COUNTRIES." Acta Horticulturae, no. 447 (October 1997): 507–14. http://dx.doi.org/10.17660/actahortic.1997.447.101.

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32

Tóth, E. T., T. Onisei, D. Amariei, and D. Lazurca. "VARIABILITY IN TISSUE CULTURE REGENERATED PLANTS OF ATROPA BELLADONNA." Acta Horticulturae, no. 289 (April 1991): 269–72. http://dx.doi.org/10.17660/actahortic.1991.289.74.

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33

M. Al- Hamdani, Kasim. "FORMATION OF Tagetes patula L. PLANTS BY TISSUE CULTURE." Mesopotamia Journal of Agriculture 35, no. 2 (2007): 20–28. http://dx.doi.org/10.33899/magrj.2007.26486.

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34

Freytag, A. H., A. P. Rao-Arelli, S. C. Anand, J. A. Wrather, and L. D. Owens. "Somaclonal variation in soybean plants regenerated from tissue culture." Plant Cell Reports 8, no. 4 (1989): 199–202. http://dx.doi.org/10.1007/bf00778531.

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35

ZHANG, Yi, and Michael PALMGREN. "Gene-editing in plants no longer requires tissue culture." Frontiers of Agricultural Science and Engineering 7, no. 2 (2020): 229. http://dx.doi.org/10.15302/j-fase-2020330.

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36

Kaeppler, S. M., and R. L. Phillips. "DNA methylation and tissue culture-induced variation in plants." In Vitro Cellular & Developmental Biology - Plant 29, no. 3 (1993): 125–30. http://dx.doi.org/10.1007/bf02632283.

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37

Lee, Chiwon W., Joel T. Nichols, Lijuan Wang, and Shanqiang Ke. "Plant Regeneration in Coreopsis lanceolata L. from Leaf Tissue Cultures." HortScience 29, no. 11 (1994): 1353–54. http://dx.doi.org/10.21273/hortsci.29.11.1353.

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Excised leaf sections of lance coreopsis cultured on Murashige Skoog (MS) medium produced adventitious shoots in response to BA. When the combinations of 0, 0.5, 1, or 2 μm NAA with 0, 5, 10, 20, or 40 μm BA were tested, shoots were induced by any of the four BA concentrations used in the medium, regardless of the presence of NAA. The average number of shoots formed per leaf section ranged from 1.4 to 4.3 seven weeks after culture initiation. Roots were induced at the base of individual shoots on the same regeneration medium when cultures were kept longer than 7 weeks. The rooted plants were t

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38

Johnston, M., I. C. Onwueme, A. J. Dowling, and B. C. Rodoni. "Comparison of suckering, leaf and corm characteristics of taro grown from tissue culture and conventional planting material." Australian Journal of Experimental Agriculture 37, no. 4 (1997): 469. http://dx.doi.org/10.1071/ea96059.

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Summary. The growth of taro plants propagated either from tissue culture plantlets or conventionally using huli (sections of corm containing the shoot apex) was followed throughout a season. The plants grown from huli began suckering 11 weeks after planting and produced an average of 5 suckers per plant. During most of the season, the huli-grown plants maintained 4–5 leaves at any one time, but had a high turnover of leaves producing 25 leaves during the 30 week period (0.8 leaves per week). At harvest the corms of the suckers contributed about one-third of the total corm weight to the entire

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39

Keith, Virginia M., and Mark H. Brand. "INFLUENCE OF CULTURE AGE, CYTOKININ LEVEL IN CULTURE, AND “RETIPPING” ON GROWTH AND THE INCIDENCE OF VARIATION IN TISSUE-CULTURED RHODODENDRONS." HortScience 27, no. 6 (1992): 585a—585. http://dx.doi.org/10.21273/hortsci.27.6.585a.

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Significant occurrences of phenotypic variation have been noted in micropropagated Rhododendron. Studies were undertaken to determine what aspects of micropropagation lead to variation. Rhododendron `Molly Fordham' was used to evaluate growth parameters and the incidence of variation in plants that originated from 3 month and 54 month old cultures. Plants from 3-month-old cultures were significantly wider than plants from 54-month-old cultures. Rhododendron `Aglo', `Molly Fordham', and `Scintillation' were used to evaluate growth and the incidence of variation in plants grown from microcutting

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40

Ikeda, Takashi, Kunio Okano, Yuka Sakamoto, and Shin-ichi Watanabe. "275 Water Relations of Fruit Cracking in Single-truss Tomato Plants." HortScience 34, no. 3 (1999): 489E—489. http://dx.doi.org/10.21273/hortsci.34.3.489e.

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This study was undertaken to investigate the water relations of tomato (Lycopersicon esculentum Mill.) fruit cracking for single-truss tomato plants. The tomato plants were cultured on a closed hydroponic system in greenhouse. Water status of culture solution and plant tissues was measured with psychrometers. Water potential of the culture solution for the stressed plant was changed from -0.06 MPa (control plants) to -0.36 MPa at 24 days after anthesis. Hardness of the fruit skin was not different significantly between the stressed plants and the control plants. Fruit cracking occurred frequen

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41

Twardovska, M. O., I. I. Konvalyuk, K. V. Lystvan, I. O. Andreev, and V. A. Kunakh. "The content of phenolic compounds and flavonoids in in vitro plants and tissue culture of Deschampsia antarctica E. Desv." Faktori eksperimental'noi evolucii organizmiv 26 (September 1, 2020): 276–81. http://dx.doi.org/10.7124/feeo.v26.1279.

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Aim. The aim of the study was a comparative assessment of total phenolic content and total flavonoid content in in vitro plants, regenerated plants, plants grown in a growth chamber, and tissue culture of several genotypes of Deschampsia antarctica. Methods. In vitro culture, Folin-Ciocalteu method, spectrophotometry, high-performance liquid chromatography. Results. The total content of phenolic compounds and total flavonoid content was determined in the samples of three D. antarctica genotypes: G/D12-2a (2n=26), DAR12 (2n=26+0–3B) and Y66 (2n=36–39). The content of these biologically active c

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42

R, Chitra. "Comparative studies on growth and Yield of Conventional and Tissue culture plants of Turmeric (Curcuma longa) var. CO2." Journal of Horticultural Sciences 14, no. 2 (2019): 162–65. http://dx.doi.org/10.24154/jhs.2019.v14i02.013.

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Turmeric (Curcuma longa L.) is an ancient spice, native of India and South East Asia used from antiquity as spice and a dye. It is commonly propagated through rhizomes. The availability of disease free quality planting material is scarce during the cropping season (June – September). An experiment was conducted to study the performance of in vitro derived turmeric plants with conventional rhizome under field condition. The results indicated that the tissue culture plants showed better performance over the conventional rhizome planting. Tissue culture plants grew vigorously and taller than conv

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43

Whelan, Ernest D. P. "Meiotic abnormalities in primary regenerants from callus culture of immature embryos of 'Norstar' winter wheat." Genome 33, no. 2 (1990): 260–66. http://dx.doi.org/10.1139/g90-040.

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Tissue culture can induce changes in chromosome structure and number in common wheat (Triticum aestivum L.). The type and frequency of such changes were evaluated in primary regenerants extracted from calli of four immature embryos of 'Norstar' winter wheat cultured for various durations. Meiotic analyses of samples from 18 or 19 primary regenerants from a single embryo cultured for 6, 10, or 14 weeks detected chromosomal changes in 17–20% of the samples. Analyses of 20 duplicate samples from these plants indicated that 7 (35%) plants were chimeras. Similar analyses for nine duplicate samples

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44

Teixeira Da Silva, Jaime A. "Novel Factors Affecting Shoot Culture of Chrysanthemum (Dendranthema × Grandiflora)." Botanica Lithuanica 20, no. 1 (2014): 27–40. http://dx.doi.org/10.2478/botlit-2014-0004.

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Abstract Teixeira da Silva J.A., 2014: Novel factors affecting shoot culture of chrysanthemum (Dendranthema × grandiflora) [Alternatyvių standiklių, skystų terpės priedų, CO2 sodrinimo ir kitų faktorių įtaka chrizantemų (Dendranthema × grandiflora (Ramat.) Kitamura) ūglių kultūrų auginimui]. - Bot. Lith., 20(1): 27-40. Chrysanthemum (Dendranthema × grandiflora (Ramat.) Kitamura) continues to be one of the most important ornamental plants in the world. Although the tissue culture of chrysanthemum has been widely explored, several unexplored topics remain, and, in developing countries, there is

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45

Linderman, Robert G., and E. Anne Davis. "Evaluation of Phytophthora ramorum in Nursery Crop Tissue Culture Propagation." Plant Health Progress 8, no. 1 (2007): 14. http://dx.doi.org/10.1094/php-2007-0822-01-rs.

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Phytophthora ramorum, cause of Ramorum blight on numerous woody ornamental shrubs, is a regulated pathogen in the US and internationally. Currently, nurseries are inspected to detect infected plants; however, many plants are propagated by tissue culture nurseries and the behavior of P. ramorum in this system is unknown. Pathogen growth and sporulation in propagation vessels containing different multiplication and rooting media, with a range of plants and without plants, was evaluated with regard to pathogen visibility and induction of disease symptoms. Within 2 weeks, the pathogen colonies wer

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Smeda, Reid J., and Stephen C. Weller. "Plant Cell and Tissue Culture Techniques for Weed Science Research." Weed Science 39, no. 3 (1991): 497–504. http://dx.doi.org/10.1017/s0043174500073288.

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Tissue and cell culture offer weed scientists many opportunities to research herbicide effects on plants. This review will discuss examples in which plant cells grown in vitro have been used to study herbicide action. Plant cell and tissue culture have many advantages over the use of whole plants; however, several disadvantages that exist are discussed. Cell cultures can be established for most plant species and provide a relatively homogeneous system for studying herbicide action. Responses of plant cells to herbicides are usually correlated with responses at the whole plant level, and cells

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Luckett, DJ, D. Rose, and E. Knights. "Paucity of somaclonal variation from immature embryo culture of barley." Australian Journal of Agricultural Research 40, no. 6 (1989): 1155. http://dx.doi.org/10.1071/ar9891155.

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Intact immature embryos of barley (cv. Golden Promise) and component tissues (the scutellum and embryonic axis) were cultured to produce callus. Regenerant plants were obtained from this callus and SC2 families raised. These families were examined in a field trial to search for somaclonal variation. No obvious variants were found confirming our previous unpublished results. The lack of somaclonal variation generated by barley tissue culture (which is in contrast to other species) was not a result of the tissue origin of the regeneration event.

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Khuspe, S. S., P. K. Gupta, D. K. Kulkarni, Urmil Mehta, and A. F. Mascarenhas. "Increased biomass production by tissue culture of eucalyptus." Canadian Journal of Forest Research 17, no. 11 (1987): 1361–63. http://dx.doi.org/10.1139/x87-210.

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Buds collected from elite fast-growing eucalyptus trees were multiplied by tissue culture. In this study, the seed (control) and the tissue culture raised plants of Eucalyptustereticornis and E. torelliana were planted in the field at two population densities. Observations of the height and diameter were taken for biomass calculation. Thirty-four months after planting, there was an overall increase in biomass of the tissue culture raised plants of both species compared with the controls. In E. tereticornis and E. torelliana, the increase in biomass was 36.9 and 49.5%, respectively, over the co

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Mohamed, Fouad, Harry Jan Swartz, and George Buta. "TISSUE CULTURE PRODUCED STRAWBERRY PLANTS ARE DEFICIENT IN ABSCISIC ACID (ABA)." HortScience 25, no. 9 (1990): 1138b—1138. http://dx.doi.org/10.21273/hortsci.25.9.1138b.

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In previous abstracts (HortScience 23:707;24:121), ABA when added throughout the in vitro production cycle, reversed the tissue culture-induced rejuvenation of the day neutral strawberry `Fern'. Compared to benzyl adenine (BA) proliferated plants, ABA treated tissue culture-produced plants flowered earlier and had more adult leaf patterns. In the present study, we analysed endogenous ABA concentrations in the apices and unexpanded leaves of BA treated tissue culture-propagated plants, selved seedlings and propagated adult runner tip plants at 3, 7 and 15 weeks ex vitro, after germination or af

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Brown, P. T. H. "DNA methylation in plants and its role in tissue culture." Genome 31, no. 2 (1989): 717–29. http://dx.doi.org/10.1139/g89-130.

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Regeneration of plants via tissue culture often results in a number of plants subsequently showing phenotypic or genotypic deviations from the parental type. This variation has been called somaclonal variation. In an analysis of regenerated Zea mays plants of the inbred line A188, high levels of phenotypic variation were observed. Subsequent analysis of these regenerated plants shows that a high proportion of the regenerants demonstrate significant alterations in the methylation status of both housekeeping and structural genes. These results are described and the theory of gene methylation is

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