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Journal articles on the topic 'Tissue culture'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Jia, Zhidong, Yuan Cheng, Xinan Jiang, et al. "3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity." BioMed Research International 2020 (March 4, 2020): 1–22. http://dx.doi.org/10.1155/2020/6354183.

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In vitro 3D hepatocyte culture constitutes a core aspect of liver tissue engineering. However, conventional 3D cultures are unable to maintain hepatocyte polarity, functional phenotype, or viability. Here, we employed microfluidic chip technology combined with natural alginate hydrogels to construct 3D liver tissues mimicking hepatic plates. We comprehensively evaluated cultured hepatocyte viability, function, and polarity. Transcriptome sequencing was used to analyze changes in hepatocyte polarity pathways. The data indicate that, as culture duration increases, the viability, function, polari

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McGreevy, Owen, Timothy Gilbert, Maria-Danae Jessel, et al. "A Preclinical Model of Human Liver Using Precision Cut Tissue Slice Culture." F1000Research 14 (June 10, 2025): 571. https://doi.org/10.12688/f1000research.162495.1.

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Preclinical models vary in complexity and cost. Traditional 2D cell cultures that are high throughput and cost effective, but lack the complexity of multicellular interactions. Animal models and more complex, but are costly, raise ethical concerns and are not a human model to better understand human disease or response to novel treatments. Human precision cut tissue slice (hPCTS) models bridge this gap, maintaining the architecture and microenvironment of original tissues. This study examines the viability and functionality of hPCTS using different tissue culture formats. Previous studies have

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Shrestha, Sunil, Vinod Kumar Reddy Lekkala, Prabha Acharya, Darshita Siddhpura, and Moo-Yeal Lee. "Recent advances in microarray 3D bioprinting for high-throughput spheroid and tissue culture and analysis." Essays in Biochemistry 65, no. 3 (2021): 481–89. http://dx.doi.org/10.1042/ebc20200150.

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Abstract Three-dimensional (3D) cell culture in vitro has proven to be more physiologically relevant than two-dimensional (2D) culture of cell monolayers, thus more predictive in assessing efficacy and toxicity of compounds. There have been several 3D cell culture techniques developed, which include spheroid and multicellular tissue cultures. Cell spheroids have been generated from single or multiple cell types cultured in ultralow attachment (ULA) well plates and hanging droplet plates. In general, cell spheroids are formed in a relatively short period of culture, in the absence of extracellu

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Peel, Trisha N., Tim Spelman, Brenda L. Dylla, et al. "Optimal Periprosthetic Tissue Specimen Number for Diagnosis of Prosthetic Joint Infection." Journal of Clinical Microbiology 55, no. 1 (2016): 234–43. http://dx.doi.org/10.1128/jcm.01914-16.

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ABSTRACTWe recently demonstrated improved sensitivity of prosthetic joint infection (PJI) diagnosis using an automated blood culture bottle system for periprosthetic tissue culture [T. N. Peel et al., mBio 7(1):e01776-15, 2016,https://doi.org/10.1128/mBio.01776-15]. This study builds on the prior research by examining the optimal number of periprosthetic tissue specimens required for accurate PJI diagnosis. Current guidelines recommend five to six, which is impractical. We applied Bayesian latent class modeling techniques for estimating diagnostic test properties of conventional culture techni

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Şükrüoğlu Erdoğan, Özge, Seda Kılıç Erciyas, Ayhan Bilir, et al. "Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma." BioMed Research International 2019 (January 17, 2019): 1–9. http://dx.doi.org/10.1155/2019/1407167.

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Epigenetic changes have major role in the normal development and programming of gene expression. Aberrant methylation results in carcinogenesis. The primary objective of our study is to determine whether primary tumor tissue and cultured tumor cells in 2D and 3D tissue culture systems have the same methylation signature forPAX5,TMPRSS2, andSBDS. These findings will play an important role in developing in vitro model system to understand the effect of methylation inhibitors on primary tumor tissue. In a previous studyPAX5,TMPRSS2, andSBDSgenes that we are investigating were reported to be methy

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Linsler, S., A. Moroldo, J. Oertel, and S. Urbschat. "P18.05.A THE GROWTH PATTERN OF MENINGIOMA CELLS IN CELL CULTURE UNDER DIFFERENT CONDITIONS." Neuro-Oncology 25, Supplement_2 (2023): ii122—ii123. http://dx.doi.org/10.1093/neuonc/noad137.413.

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Abstract BACKGROUND Cell cultures is an established method in tumor research. Thereby, the growth rate is important for the results. Depending on the experimental and clinical setting, the used tissue is exposed to different conditions. Interestingely, the different cell and tissue conditions are not well reported yet. Although, it is likely that the different conditions influence the growth pattern of cell cultures in primary cell lines. Here, the authors present their experimental analysis of cell culture of primary meningioma cells after different initial tissue conditions. MATERIAL AND MET

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Stokes, Rebecca A., Michelle C. Coleman, Artem S. Rogovskyy, Vanna M. Dickerson, and Kelley M. Thieman Mankin. "Comparison of bacteriologic culture results for skin wound swabs and skin wound biopsy specimens." Journal of the American Veterinary Medical Association 259, no. 12 (2021): 1416–21. http://dx.doi.org/10.2460/javma.20.10.0568.

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Abstract OBJECTIVE To compare bacteriologic culture results for superficial swab and tissue biopsy specimens obtained from dogs with open skin wounds. ANIMALS 52 client-owned dogs. PROCEDURES For each dog, 1 wound underwent routine preparation prior to collection of 2 specimens, 1 by superficial swab (Levine) technique and 1 by tissue biopsy. Specimens were processed for bacteriologic culture. Two observers determined whether any detected difference in culture results for the 2 types of specimen would have resulted in differing treatment plans. RESULTS Culture results of swab and tissue biopsy

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Lee, Yoonjung, Ahrang Lee, Hae Seong Jeong, et al. "The microbiology of periprosthetic joint infections as revealed by sonicate cultures in Korea: Routine use of fungal and mycobacterial cultures is necessary?" PLOS ONE 19, no. 8 (2024): e0309046. http://dx.doi.org/10.1371/journal.pone.0309046.

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Background Although sonication is a valuable diagnostic tool for periprosthetic joint infections (PJI), it is not commonly utilized. We analyzed sonicate and intraoperative tissue culture results obtained from three hospitals to define the microbial etiology of PJIs in Korea. Furthermore, we investigated necessity of conducting regular fungal and mycobacterial cultures. Methods We retrospectively analyzed data for patients with suspected orthopedic-related infections between 2017 and 2022, who had undergone prostheses removal surgery. We included 193 patients with suspected PJIs, and bacterial

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9

Prakash, Jitendra. "Plant Tissue Culture." Nature Biotechnology 9, no. 7 (1991): 607. http://dx.doi.org/10.1038/nbt0791-607.

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10

Ezzell, Carol. "Tissue culture tools." Nature 327, no. 6119 (1987): 256–58. http://dx.doi.org/10.1038/327256a0.

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Prince, Cary. "Tissue culture trends." Nature 339, no. 6224 (1989): 488–90. http://dx.doi.org/10.1038/339488a0.

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Ezzell, Carol. "Tissue culture tips." Nature 333, no. 6173 (1988): 580–82. http://dx.doi.org/10.1038/333580a0.

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13

de Fossard, Ronald A. "Tissue-culture guide." Trends in Plant Science 6, no. 2 (2001): 85. http://dx.doi.org/10.1016/s1360-1385(00)01856-2.

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Dagla, H. R. "Plant tissue culture." Resonance 17, no. 8 (2012): 759–67. http://dx.doi.org/10.1007/s12045-012-0086-8.

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Seil, Fredrick J. "Tissue Culture Models of Myelination After Oligodendrocyte Transplantation." Journal of Neural Transplantation 1, no. 2 (1989): 49–55. http://dx.doi.org/10.1155/np.1989.49.

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Studies of myelination after transplantation of mature oligodendrocytes to cerebellar cultures in which oligodendrocyte maturation and myelination had been irreversibly inhibited by exposure to cytosine arabinoside were reviewed. Transplanted oligodendrocytes were derived from three sources, including cerebellar explants treated with kainic acid, dissociated oligodendrocyte cultures, and optic nerve fragments. Oligodendrocytes from all sources migrated into the host explants and myelinated appropriate axons. The time of appearance of myelin and the percentage of host cultures myelinated differ

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Tanini, Annalisa, Maria Luisa Brandi, Umberto Modigliani, Carlo M. Rotella, and Roberto Toccafondi. "Transient lack of response to TSH of human cultured thyroid cells obtained from hyperfunctioning tissue." Acta Endocrinologica 113, no. 3 (1986): 346–54. http://dx.doi.org/10.1530/acta.0.1130346.

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Abstract. TSH-induced cAMP accumulation in cells obtained from normal and pathological thyroid tissue was studied during the first 12 days of primary culture. In normal thyroid tissue cultures (N = 7), the response of cAMP to TSH was present from the second day of culture and reached its maximum after 8 days. A similar behaviour was observed in cultures obtained from euthyroid sporadic goitres (N = 8), even if the rate of response was slightly lower than that of normal tissue. Similarly, cultured cells from euthyroid 'autonomous' nodules (N = 8) appeared to be responsive to TSH during the peri

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Cheng, Ya-Fu, Ching-Yuan Cheng, Chang-Lun Huang, Wei-Heng Hung, and Bing-Yen Wang. "Pleural Peels Tissue Culture plus Pleural Fluid Culture Help to Improve Culture Rate for Empyema." Journal of Clinical Medicine 11, no. 7 (2022): 1882. http://dx.doi.org/10.3390/jcm11071882.

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Background: Empyema is known as a serious infection, and outcomes of empyema cases remain poor. Pleural fluid culture and blood culture have been reported to give unsatisfactory results. We introduce a novel pleural peels tissue culture during surgery and aim to improve the culture results of empyema. Methods: This was a retrospective study and was obtained from our institute. Patients with stage II or III empyema undergoing video-assisted thoracic surgery decortication from January 2019 to June 2021 were included in the study. Results: There were 239 patients that received a pleural peels tis

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Souvik, Kundu, Sindhusha Pedada, and Kumar Anil. "Micro-propagation techniques in horticultural crops and various factors affecting it: A review." Modern Phytomorphology 16, no. 1 (2022): 4–8. https://doi.org/10.5281/zenodo.7731512.

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A German Botanist, Gottlieb Haberlandt, made the first attempt to use the in vitro method to grow plant tissues and gave the basic concept for the cultivation of plant cells, tissues and organs in vitro culture over 100 years ago. At the initial period plant tissue cultures treated as a research tool and only focused on study the development of small, isolated cells and segments of plant tissues. At the pinnacle of the plant tissue culture period during the 1980s, in a moderately brief timeframe, many commercial laboratories were set up to capitalize by the capability of micropropagation for l

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Sonwane, Ritesh, Rupesh Patil, Ketan Sonwane, Bhavesh Raghuwanshi, and Pragati Lokhande. "Tissue Culture: Tissue Plants Booking Website." Advancement of IoT in Blockchain Technology and its Applications 1, no. 1 (2022): 8–10. http://dx.doi.org/10.46610/aibtia.2022.v01i01.002.

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Tissue Culture is an website for benefit of the farming community .Where farmers can book Tissue Plants at anytime from anywhere in this paper we discuss about a Website, from where Farmers can easily Book Plants like Banana, Papaya, strawberry, Pine apple etc. at cheap price and take higher yield form their crops.

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Imashiro, Chikahiro, Kai Yamasaki, Ryu-ichiro Tanaka, Yusuke Tobe, Katsuhisa Sakaguchi, and Tatsuya Shimizu. "Perfusable System Using Porous Collagen Gel Scaffold Actively Provides Fresh Culture Media to a Cultured 3D Tissue." International Journal of Molecular Sciences 22, no. 13 (2021): 6780. http://dx.doi.org/10.3390/ijms22136780.

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Culturing three-dimensional (3D) tissues with an appropriate microenvironment is a critical and fundamental technology in broad areas of cutting-edge bioengineering research. In addition, many technologies have engineered tissue functions. However, an effective system for transporting nutrients, waste, or oxygen to affect the functions of cell tissues has not been reported. In this study, we introduce a novel system that employs diffusion and convection to enhance transportation. To demonstrate the concept of the proposed system, three layers of normal human dermal fibroblast cell sheets are u

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Ahlawat, Anju. "Guar Species Regenerated through Plant Tissue Culture Technique." International Journal of Science and Research (IJSR) 11, no. 9 (2022): 276–81. http://dx.doi.org/10.21275/sr22826105425.

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Potts, Robert W. A., Alejandro P. Gutierrez, Yennifer Cortés-Araya, Ross D. Houston, and Tim P. Bean. "Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas." PeerJ 8 (June 1, 2020): e9180. http://dx.doi.org/10.7717/peerj.9180.

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Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture

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Ogita, Shinjiro, Junko Miyazaki, Toshinari Godo, and Yasuo Kato. "Possibility for Selective Accumulation of Polyphenolics in Tissue Cultures of Senno (Lychnis Senno Siebold et Zucc.)." Natural Product Communications 4, no. 3 (2009): 1934578X0900400. http://dx.doi.org/10.1177/1934578x0900400312.

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Senno ( Lychnis senno Siebold et Zucc.), a traditional ornamental plant in Japan had been used as a crude drug acting as natural blood thinners. Since tissue culture protocols have been established, we analyzed polyphenol accumulation profiles in shoot culture, multiple shoot culture, and callus culture using the technique of HPLC with a Photodiode Array Detector. By comparing the HPLC profiles at 220-400 nm from extracts of different cultures, 14 putative flavonoids were confirmed as major metabolites in the cultures of Senno. Among the 14 compounds detected, 6 were tissue specific metabolite

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Peel, Trisha N., John A. Sedarski, Brenda L. Dylla, et al. "Laboratory Workflow Analysis of Culture of Periprosthetic Tissues in Blood Culture Bottles." Journal of Clinical Microbiology 55, no. 9 (2017): 2817–26. http://dx.doi.org/10.1128/jcm.00652-17.

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ABSTRACTCulture of periprosthetic tissue specimens in blood culture bottles is more sensitive than conventional techniques, but the impact on laboratory workflow has yet to be addressed. Herein, we examined the impact of culture of periprosthetic tissues in blood culture bottles on laboratory workflow and cost. The workflow was process mapped, decision tree models were constructed using probabilities of positive and negative cultures drawn from our published study (T. N. Peel, B. L. Dylla, J. G. Hughes, D. T. Lynch, K. E. Greenwood-Quaintance, A. C. Cheng, J. N. Mandrekar, and R. Patel, mBio 7

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Okano, T. "Muscular tissue engineering: capillary-incorporated hybrid muscular tissues in vivo tissue culture." Cell Transplantation 7, no. 5 (1998): 435–42. http://dx.doi.org/10.1016/s0963-6897(98)00030-x.

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Okano, Takahisa, and Takehisa Matsuda. "Muscular Tissue Engineering: Capillary-Incorporated Hybrid Muscular Tissues in Vivo Tissue Culture." Cell Transplantation 7, no. 5 (1998): 435–42. http://dx.doi.org/10.1177/096368979800700502.

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Requirements for a functional hybrid muscular tissue are 1) a high density of multinucleated cells, 2) a high degree of cellular orientation, and 3) the presence of a capillary network in the hybrid tissue. Rod-shaped hybrid muscular tissues composed of C2C12 cells (skeletal muscle myoblast cell line) and type I collagen, which were prepared using the centrifugal cell-packing method reported in our previous article, were implanted into nude mice. The grafts, comprised three hybrid tissues (each dimension, diameter, approximately 0.3 mm, length, approximately 1 mm, respectively), were inserted

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Mothersill, Carmel, Colin Seymour, M. J. Moriarty, and M. J. Cullen. "Long-term culture of differentiated human thyroid tissue." Acta Endocrinologica 108, no. 2 (1985): 192–99. http://dx.doi.org/10.1530/acta.0.1080192.

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Abstract. Human thyroid cells obtained during surgery have been maintained in monolayer culture for at least 2 months and without loss of morphological or functional differentiation. Samples as small as 0.5 g could be cultured but best results were obtained with samples of 5–10 g. The technique used was developed in this laboratory for sheep tissue and was applicable without significant modification to human tissue. It depends on the complete absence of media changes at any time during the culture period. Energy substrates are replenished by the addition of concentrated glucose solutions to th

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Xiang, Yu, Jiongyi Yan, Xujin Bao, Andrew Gleadall, Paul Roach, and Tao Sun. "Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering." International Journal of Molecular Sciences 24, no. 6 (2023): 5234. http://dx.doi.org/10.3390/ijms24065234.

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Developmental engineering (DE) aims to culture mammalian cells on corresponding modular scaffolds (scale: micron to millimeter), then assemble these into functional tissues imitating natural developmental biology processes. This research intended to investigate the influences of polymeric particles on modular tissue cultures. When poly(methyl methacrylate) (PMMA), poly(lactic acid) (PLA) and polystyrene (PS) particles (diameter: 5–100 µm) were fabricated and submerged in culture medium in tissue culture plastics (TCPs) for modular tissue cultures, the majority of adjacent PMMA, some PLA but no

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Beck, K. L., J. Singh, and M. Anzar. "47 THE AVIAN CHORIO-ALLANTOIC MEMBRANE: A SUITABLE SHORT-TERM CULTURE SYSTEM FOR BOVINE OVARIAN TISSUE." Reproduction, Fertility and Development 27, no. 1 (2015): 116. http://dx.doi.org/10.1071/rdv27n1ab47.

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Successful cryopreservation of bovine ovarian tissue holds enormous potential for long-term maintenance of female gametes to preserve genetic diversity by tissue banking. Traditionally, in vitro culture followed by histopathological examination has been used to assess the post-thaw viability of cryopreserved tissues. Recently, in ovo transplantation of mammalian tissues on the chorio-allantoic membrane (CAM) of a growing chicken embryo has emerged as an alternative method for short-term culture. The purpose of this experiment was to compare CAM culture of bovine ovarian tissue over a 5-day per

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Dey, Nandini, Yuliang Sun, Amy K. Krie, et al. "Three dimensional organotypic ex vivo culture of tissues from post-operated tumor samples: Strengths and limitations." Journal of Clinical Oncology 35, no. 15_suppl (2017): e23151-e23151. http://dx.doi.org/10.1200/jco.2017.35.15_suppl.e23151.

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e23151 Background: Evolution of tumor occurs in two phases, pretreatment phase, and posttreatment phase.Tumorigenic history and path of tumorigenic evolution determine the response of tumor cells to antitumor drug and thus the outcome of treatment. Carcinomas from the same organ-site with similar genomic alterations in different patients may vary in their tumorigenic history and their diverse paths of tumorigenic evolution which cause them respond differently to the same antitumor drugs. Uniqueness of tumorigenic history and paths of tumorigenic evolution in individual patient makes it ideal t

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Winkelkotte, Maximilian, Florian Schmieder, Stephan Behrens, et al. "Micro-Physiological-Systems enable investigation of hypoxia induced pathological processes in human aortic valve cells and tissues." Current Directions in Biomedical Engineering 7, no. 2 (2021): 45–48. http://dx.doi.org/10.1515/cdbme-2021-2012.

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Abstract Aortic valve (AV) stenosis is characterized by tissue fibrosis and calcification. Fibrous thickening can result in reduced tissue oxygen supply leading to pathological valvular interstitial cell (VIC) differentiation and calcification. Static 2D VIC cultures and animal models are limited in the ability to reflect human AV calcification. Culturing of VICs in micro-physiological-systems (MPS) in a pulsatile flow and the establishment of a modular AV tissue incubation chamber (TIC) are new approaches to evaluate pathophysiological processes of AV disease. Therefore, a MPS able to adjust

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Kreß, Sebastian, Roland Schaller-Ammann, Jürgen Feiel, et al. "Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures." Cells 11, no. 3 (2022): 412. http://dx.doi.org/10.3390/cells11030412.

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The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform combining a perfusion bioreactor with an integrated minimally invasive sampling system and implemented sensors that enables the online monitoring of culture parameters and medium compounds within 3D cultures. As a proof-of-concept, primary cells as well

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Anushi, Shubham Jain, Manjunath Rathod, et al. "Plant Tissue Culture for Medical Therapy: Unlocking the Potential of Medicinal Plants." Current Journal of Applied Science and Technology 42, no. 46 (2023): 7–22. http://dx.doi.org/10.9734/cjast/2023/v42i464289.

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Plant tissue culture is emerging as a pivotal biotechnological tool with profound implications for medical therapy, particularly in the realm of herbal medicine. Medicinal plants have long been cherished for their natural healing properties. However, escalating demand, habitat destruction, and overharvesting have threatened the availability and sustainability of these valuable resources. Plant tissue culture addresses these concerns by enabling the mass propagation of medicinal plants. In controlled environments, plant tissues can be multiplied rapidly, providing a continuous and sustainable s

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Das, Debarath, Kishore D, Lakshmana R, Pravin Dhas, Snigdha N, and Malarmannan M. "Comparative study of tissue culture and sensitivity versus swab culture and sensitivity of microorganisms in the healing of diabetic foot ulcers." International Journal of Research in Pharmaceutical Sciences 15, no. 1 (2024): 25–31. http://dx.doi.org/10.26452/ijrps.v15i1.4660.

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This prospective observational study, conducted at the Department of General Surgery, SRM Medical College and Hospital, aimed to assess the effectiveness of tissue culture and sensitivity compared to swab culture and sensitivity in the healing of diabetic foot ulcers through antibiotic sensitivity of microorganisms. Between May 2016 and August 2017, 160 subjects with diabetic foot ulcers were randomly assigned treatment based on either swab or tissue culture findings. Patients were followed at 15-day intervals for up to 60 days. Results showed positive swab cultures in 76.88% and positive tiss

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Vogt, P. M., F. W. Peter, and H. U. Steinau. "Perspectives of tissue transfer and tissue culture." Der Orthopäde 27, no. 1 (1998): 45–50. http://dx.doi.org/10.1007/pl00003449.

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Kunakh, V. A., D. O. Navrotska, M. O. Twardovska, and I. O. Andreev. "Peculiarities of chromosomal variability in cultured tissues of Deschampsia antarctica Desv. plants with different chromosome numbers." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 14, no. 1 (2016): 36–43. http://dx.doi.org/10.7124/visnyk.utgis.14.1.542.

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Aim. To clarify the details of chromosome variation in calli derived from D. antarctica plants in the initial passages of the culture in vitro. Methods. Induction of callus from root explants of plants, which were grown from seeds, and consequent subcultivation of tissue culture. Cytogenetic analysis of squashed slides stained by acetic-orcein and counting the number of chromosomes in mitotic metaphase plates. Results. There were analyzed the cultured tissues derived from D. antarctica plants with different chromosome numbers: diploid plants (2n=26), mixoploid plant with B-chromosomes (2n=26+1

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Polisetti, Naresh, Gottfried Martin, Eva Ulrich, et al. "Influence of Organ Culture on the Characteristics of the Human Limbal Stem Cell Niche." International Journal of Molecular Sciences 24, no. 23 (2023): 16856. http://dx.doi.org/10.3390/ijms242316856.

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Organ culture storage techniques for corneoscleral limbal (CSL) tissue have improved the quality of corneas for transplantation and allow for longer storage times. Cultured limbal tissue has been used for stem cell transplantation to treat limbal stem cell deficiency (LSCD) as well as for research purposes to assess homeostasis mechanisms in the limbal stem cell niche. However, the effects of organ culture storage conditions on the quality of limbal niche components are less well described. Therefore, in this study, the morphological and immunohistochemical characteristics of organ-cultured li

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Encina, C. L., I. M. G. Padilla, J. M. Cazorla, and E. Caro. "TISSUE CULTURE IN CHERIMOYA." Acta Horticulturae, no. 497 (August 1999): 289–302. http://dx.doi.org/10.17660/actahortic.1999.497.15.

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YAMAMOTO, Yoshikazu. "Tissue Culture of Lichens." Plant tissue culture letters 5, no. 1 (1988): 44–46. http://dx.doi.org/10.5511/plantbiotechnology1984.5.44.

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Loeb, Marcia, and Jun Mitsuhashi. "Invertebrate Tissue Culture Methods." Annals of the Entomological Society of America 96, no. 3 (2003): 344. http://dx.doi.org/10.1603/0013-8746(2003)096[0344:itcm]2.0.co;2.

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Illg, Rolf Dieter. "Plant tissue culture techniques." Memórias do Instituto Oswaldo Cruz 86, suppl 2 (1991): 21–24. http://dx.doi.org/10.1590/s0074-02761991000600008.

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HORTON, BRENDAN. "Cell and tissue culture." Nature 381, no. 6579 (1996): 255–58. http://dx.doi.org/10.1038/381255a0.

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Ruben, Regina L. "The new tissue culture." Science 356, no. 6335 (2017): 342. http://dx.doi.org/10.1126/science.356.6335.342.

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Hickey, Ivor. "Tissue culture without tears." Trends in Cell Biology 3, no. 12 (1993): 453. http://dx.doi.org/10.1016/0962-8924(93)90042-y.

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Messenger, A. G. "Hair follicle tissue culture." British Journal of Dermatology 113, no. 6 (1985): 639–40. http://dx.doi.org/10.1111/j.1365-2133.1985.tb02397.x.

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Poginsky, B., W. Preil, J. Westendorf, and Lj Kraus. "Tissue Culture ofRubia tinctorum." Planta Medica 55, no. 02 (1989): 231–32. http://dx.doi.org/10.1055/s-2006-961989.

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J. Robins, Ichard. "Plant tissue culture manual." Phytochemistry 31, no. 9 (1992): 3301–2. http://dx.doi.org/10.1016/0031-9422(92)83507-u.

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Brückner, Luzi, Annika Reinshagen, Bahriye Aktas, Ingo Bechmann, Ivonne Nel, and Sonja Kallendrusch. "Development of a personalized ex vivo drug screening test for patients with ovarian cancer." Journal of Clinical Oncology 38, no. 15_suppl (2020): e18090-e18090. http://dx.doi.org/10.1200/jco.2020.38.15_suppl.e18090.

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e18090 Background: The standard therapy of patients with ovarian cancer consists of primary surgery followed by chemotherapy. Initial response rates are very high, but recurrence occurs in 85% of the cases. Personalized ex vivo analyses of various anti-tumor compounds in a standardized tissue slice culture system (1) might be a very promising approach for individualized therapeutic decisions. In comparison to cell culture, tumor slice cultures maintain the direct tumor microenvironment which plays a role in resistance mechanisms and thus therapy response. Methods: Patient derived tumor culture

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Martin-Iglesias, Sara, Lara Milian, María Sancho-Tello, et al. "BMP-2 Enhances Osteogenic Differentiation of Human Adipose-Derived and Dental Pulp Stem Cells in 2D and 3D In Vitro Models." Stem Cells International 2022 (March 4, 2022): 1–15. http://dx.doi.org/10.1155/2022/4910399.

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Bone tissue provides support and protection to different organs and tissues. Aging and different diseases can cause a decrease in the rate of bone regeneration or incomplete healing; thus, tissue-engineered substitutes can be an acceptable alternative to traditional therapies. In the present work, we have developed an in vitro osteogenic differentiation model based on mesenchymal stem cells (MSCs), to first analyse the influence of the culture media and the origin of the cells on the efficiency of this process and secondly to extrapolate it to a 3D environment to evaluate its possible applicat

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Mahato, Asmita, Goma Chaudhari, Gaurave Banjade, and Shreesha Uprety. "TISSUE CULTURE AND ITS APPLICATION IN MODERN AGRICULTURE." i TECH MAG 5 (January 3, 2023): 09–11. http://dx.doi.org/10.26480/itechmag.05.2023.09.11.

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Tissue culture is a technique of growing plant cells, tissues and organs in an artificial prepared medium under aseptic conditions, which was invented by Gottlieb Haberlandt in 1902. Plant tissue culture is based on cellular totipotency, dedifferentiation, and redifferentiation. This paper overviews about procedures and applications of tissue culture by the analysis of numerous published studies. Compared to normal plant breeding methods, which take substantially longer, crops produced through tissue culture is developed through accurate and time-saving methods. It enables the recovery of embr

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