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1
Becker, Thomas. "Preprotein recognition and translocation by the Toc complex." Diss., lmu, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-39523.
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Becker, Thomas Verfasser], and Jürgen [Akademischer Betreuer] [Soll. "Preprotein recognition and translocation by the Toc complex / Thomas Becker. Betreuer: Jürgen Soll." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2005. http://d-nb.info/1108093329/34.
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Becker, Thomas [Verfasser], and Jürgen [Akademischer Betreuer] Soll. "Preprotein recognition and translocation by the Toc complex / Thomas Becker. Betreuer: Jürgen Soll." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-39523.
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Yang, Ronghua. "Studies on value distribution of solutions of complex linear differential equations /." Joensuu : Joensuun yliopistopaino, 2006. http://www.loc.gov/catdir/toc/fy0706/2006421381.html.
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Almeida, Roberto Barbosa de. "Purification and characterization of LOX isoenzymes from germinating barley : biotransformation of complex lipids /." [S.l. : s.n.], 2005. http://www.gbv.de/dms/bs/toc/506584569.pdf.
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El-Souefi, Sameh. "Challenging complex realities : the paradiplomacy of the Palestinian minority citizens of the State of Israel? /." Helsinki : Dept. of Political Science / Faculty of Social Sciences, University of Helsinki, 2004. http://www.loc.gov/catdir/toc/fy0612/2006402063.html.
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Schürmann, Jörg. "Topology of singular spaces and constructible sheaves /." Basel [u.a.] : Birkhäuser, 2003. http://www.loc.gov/catdir/toc/fy0803/2003062963.html.
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Newton, David William. "Physical studies of including drugs within complex coacervates of gelatin-acacia to produce microglobules for use in parenteral pharmaceutical dosage forms /." Ann Arbor,Mich. : University Microfilms International, 1992. http://www.gbv.de/dms/bs/toc/016106555.pdf.
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Benz, Johan Philipp. "Characterization of two components of the chloroplastic Tic complex." Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-112160.
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Stan, Tincuta. "The Translocase of the Outer Membrane of Mitochondria (TOM complex)." Diss., lmu, 2003. http://nbn-resolving.de/urn:nbn:de:bvb:19-14479.
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Schuck, Natalie. "Functional analysis of two components of the chloroplastic TIC complex." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-164589.
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Barandas, Guilherme Mayrink. "Caracterização de Bacilos Gram-Negativos Não Fermentadores não usuais em bacteremias pelas técnicas de Matrix-Assisted Laser Desorption IonizationTime of Flight Mass Spectrometry, sequenciamento de DNA e método fenotípico convencional." Universidade do Estado do Rio de Janeiro, 2013. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9548.
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Conselho Nacional de Desenvolvimento Científico e TecnológicoAlguns Bastonetes Gram-negativos não fermentadores (BGNNF) costumam ser considerados clinicamente pouco significantes e a sua implicação em infecções é subestimada. Devido à similaridade fenotípica, mudanças taxonômicas, baixa reatividade bioquímica e limitações nos bancos de dados em sistemas comerciais, a identificação de BGNNF é frequentemente equivocada, culminando com a denominação de diferentes micro-organismos apenas como BGNNF, por falta de melhor diferenciação. O objetivo desse estudo foi avaliar, por métodos fenotípico convencional, proteômico e molecular, a identificação de BGNNF incomuns isolados em hemoculturas de pacientes atendidos em um hospital universitário no Rio de Janeiro. Foram selecionadas 78 amostras isoladas de hemoculturas caracterizadas no laboratório clinico como BGNNF para a identificação por sequenciamento dos genes 16S RNA e recA, por um conjunto amplo de testes fenotípicos manuais e por MALDI-TOF MS. Os micro-organismos predominantes na amostragem foram genotipados pela técnica de eletroforese em gel de campo pulsado (PFGE). Pelo sequenciamento do gene 16S rRNA, a maioria das amostras (n=31; 40%) foi incluída no gênero Burkholderia, seguido de Pseudomonas stutzeri (10%) e Delftia acidovorans (4%). Os demais isolados foram agrupados em 27 diferentes espécies. O sequencimento do gene recA identificou a maioria das espécies de Burkholderia como Burkholderia contaminans (n=19; 24%). Os testes fenotípicos incluíram as 31 amostras apenas no CBc e para as outras 47 amostras, a concordância com o sequenciamento do gene 16S rRNA em nível de espécie foi de 64% (n=30) e apenas em gênero a concordância foi de 17% (n=8). A análise comparativa geral da identificação por MALDI-TOF MS com o sequenciamento do gene16S rRNA mostrou que 42% (n=33) das 78 amostras foram concordantes em nível de espécie e 45% (n=35) apenas em gênero. Excluindo as amostras do CBc, houve um aumento da concordância em nível de espécie para 60%. As discordâncias parecem ser devido às diferenças nos perfis proteicos das amostras em relação às amostras-referência do banco de dados do equipamento e podem ser aprimorados com a atualização de perfis no sistema. A análise do polimorfismo genético de B. contaminans mostrou a ausência de um clone disseminado causando surto, além da provável origem ambiental das infecções. Os setores de nefrologia e hemodiálise contribuíram com maior número de pacientes com amostras positivas (5 pacientes e 9 amostras). Os grupos clonais BcoD e BcoE foram encontrados em pacientes assistidos no mesmo setor com diferença de quatro meses (BcoD, nefrologia) e 1,5 ano (BcoE, hemodilálise), entre as culturas, respectivamente. As discordâncias entre as técnicas ocorreram principalmente devido a dificuldade de identificação das espécies do CBc. Os BGNNF incomuns são de difícil caracterização independente da metodologia usada e nenhum método por si só foi capaz de identificar todas as amostras.
Some nonfermenting Gram-negative Bacilli (NFGNB) are considered of low clinical significance, and their implication in infections is usually underestimated. Due to their phenotypic similarities, frequent taxonomic changes and low biochemical reactivity, as well as to limitations of bacterial identification commercial system databases, these NFGNB are frequently misidentified and are collectively referred to as NFGNB group, in the lack of a better differentiation. The aim of the present study was to evaluate the performance of the conventional phenotypic method, the proteomic matrix-assisted laser desorption ionization time of flight mass spectometry method (MALDI-TOF MS) and of molecular methods (16S RNA and recA gene sequencing) in the identification of 78 unusual NFGNB isolated from blood cultures of pacients treated at an university hospital in Rio de Janeiro. Clonality of the predominant species identified within these isolates was determined by pulsed-field gel electrophoresis (PFGE). By the 16S rRNA gene sequence analysis, most strains (n = 31; 40%) were included in the Burkholderia spp. followed by Pseudomonas stutzeri (n = 8; 10%), Delftia acidovorans (n = 3; 4%) and Stenotrophomonas maltophilia (n = 3; 4%). The remaining bacterial isolates were included in 27 different species. By the recA gene sequencing technique, most bacteria from the Burkholderia cepacia complex (BCC), samples were classified as Burkholderia contaminans (n=19; 24%). Phenotypic tests provided accurate identification of all 31 isolates included in the BCC by the 16S rRNA gene sequence analysis. For the other 47 samples, agreement of the results obtained with these two techniques in species and genus level identifications occurred in 30 (63,8%) and 17 samples (36,2%), respectively. The results obtained by the MALDI-TOF MS and 16S rRNA gene sequencing methods agreed at species and genus levels in 33 (42%) and 35 isolates (45%), respectively. When bacteria from the BCC were excluded from the analysis, the agreement between the two techniques at species level increased to 60%. Misidentification by the MALDI-TOF MS method may be due to differences in protein spectra between the samples and the reference strains in the equipment database. PFGE analysis of B. contaminans isolates revealed the absence of a disseminate clone causing an outbreak, and the probable environmental source of infections. The nefrology ang dialisis sectors contributed to the greatest number of patients with positive cultures (5 pacients and 9 isolates). Clones BcoD and BcoE were found in blood cultures of pacientes treated in a same sector with differences of 4 months (BcoD, nefrology) and 1.5 year (BcoE, dialisis). The misidentifications occurred mainly due to the hard differentiation of BCC species. Unusual NFGNB are of difficult characterization whatever the methodology used and no method alone was able to identify all the isolates.
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Popov-Celeketic, Jelena. "The function of Mim1 in the biogenesis of the mitochondrial TOM complex." Diss., lmu, 2008. http://nbn-resolving.de/urn:nbn:de:bvb:19-94819.
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Carr, Stephen B. "Structural studies of the inhibition and translocation into Escherichia coli of a ribosome inactivating colicin." Thesis, University of East Anglia, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327494.
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Khedairy, Hamid S. (Hamid Sabri). "Determination of the complete nucleotide sequence of the xylZ region of the Pseudomonas putida TOL plasmid pDK1, encoding a subunit of the toluate oxidase complex." Thesis, University of North Texas, 1990. https://digital.library.unt.edu/ark:/67531/metadc798448/.
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A 1.57 kb XhoI restriction fragment derived from the TOL plasmid pDKI was subcloned into the E. Coli plasmid pUC19. The complete nucleotide sequence of this XhoI fragment was determined using both the chemical cleavage and chain termination DNA sequencing methods.
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Paxton, Thanai. "Ultra-high sensitivity unambiguous sequencing on a novel geometry quadrupole orthogonal-acceleration time of flight mass spectrometer, the Q-TOF." Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322004.
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Mulay, Ajay A. "Participatory Strategic Planning System for Complex Service Environments." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1314736354.
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Dolman, Richard. "Physical properties derived from seismic modelling at the toe of the Barbados accretionary complex." Thesis, University of Birmingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364522.
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Bouveret, Emmanuelle. "Mécanisme d'importation des colicines via le complexe Tol/PAL chez Escherichia coli." Aix-Marseille 2, 1998. http://www.theses.fr/1998AIX22036.
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Les colicines sont des toxines proteiques produites par escherichia coli et actives sur escherichia coli. Afin de penetrer les cellules cibles, elles doivent traverser l'enveloppe de la bacterie constituee de deux membranes separees par un espace dit periplasmique. Pour cela, ces toxines ont parasite un complexe proteique de l'enveloppe : le complexe tol/pal. Le domaine n-terminal des colicines est implique dans cette etape d'importation egalement appelee translocation. Nous avons demontre l'existence d'interactions in vivo entre le domaine de translocation de la colicine a et les proteines tola et tolb et entre le domaine de translocation de la colicine e3 et tolb. Dans le cas de la colicine a, les interactions se font par des domaines distincts qui ont ete localises par mutagenese aleatoire et dirigee. Parallelement, l'organisation du complexe tol a ete etudiee. L'interaction entre tolb et pal qui est une lipoproteine de la membrane externe explique la localisation de tolb dans les membranes. L'interaction de tolb avec les porines trimeriques nous permet d'envisager un role du systeme tol/pal dans la biogenese de la membrane externe. Finalement, un projet de cristallisation de la proteine tolb a ete amorce. Des cristaux diffractants a 2 angstrom de resolution ont ete obtenus et nous font esperer la determination de la structure de cette proteine pour bientot.
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Schuck, Natalie [Verfasser], and Jürgen [Akademischer Betreuer] Soll. "Functional analysis of two components of the chloroplastic TIC complex / Natalie Schuck. Betreuer: Jürgen Soll." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2013. http://d-nb.info/104601160X/34.
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Trindade, Gustavo F. "The development of multivariate analysis methodologies for complex ToF-SIMS datasets : applications to materials science." Thesis, University of Surrey, 2018. http://epubs.surrey.ac.uk/846328/.
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Secondary ion mass spectrometry (SIMS) is a technique that has evolved to be one of the most powerful techniques for the analysis of organic samples. Modern instruments are capable of obtaining three-dimensional information with high spatial resolution of a material with information as rich as a full mass spectrum at every voxel of the 3D structure, thus generating very large and complex datasets. Multivariate analysis (MVA) methods are used within the SIMS community, however, the absence of MVA in the software packages of instrument manufacturers together with constant increase in data and data analysis complexity demands practical data analysis solutions that are accessible to scientists of diverse backgrounds. This thesis aims to expand the applicability of three major MVA methods to complex SIMS datasets: Principal component analysis (PCA), non-negative matrix factorisation (NMF) and k-means clustering. This is achieved by establishing and validating existing and novel methodologies for the processing of large and complex datasets. Furthermore, it presents the development of a software that encompasses these methodologies and provide accessible and flexible analysis and data visualisation tools. Finally, it presents the application of the software to a series of experiments carried out at The Surface Analysis Laboratory of the University of Surrey in which data processing enabled deeper interpretation of the results and helped to achieve insights towards scientific and industrial problem solving.
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Behl, Joshua. "Education is Too Complex to Simply be Complicated: Repellors, Attractors, and the Interconnectedness of Things." Diss., North Dakota State University, 2018. https://hdl.handle.net/10365/28786.
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Our classrooms exist in a reality of increasing interconnectedness, cultural and societal boundary shifts, and readily available (mis)information. This reality thrusts learners and the classroom into a diverse arena where our collective and individual demographics influence and impact those around us, how we grow to understand ?others,? and understand who we are in that context. It is in this context that exists an opportunity to conceptualize a new model to make explicit the complex, dynamic, self-similar relationships, and emergent contexts in which we operate within the classroom ecosystem. This ecosystem is one that is connected in complex and dynamic ways, understandable only through holistic analysis. While the classroom, the individual agents that comprise it, and a myriad of other aspects or properties of the greater system all contribute to the classroom experience, none of them independently defines the system, controls the behaviors of it participants, or dictates the trajectories of learning that occur within it. It is conceptualized that within the complex system that is a classroom, there exists phenomena that influence and direct the learning trajectories of the class and its individual students. It is further believed that some plenary amongst these influencing phenomena are attractors and repellors. The main question this research intends to answer was, ?What are the distinct types and behaviors of attractors and repellors that exist in the classroom context when viewing that classroom as a complex system?? This research not only confirmed that classrooms operate and exist as complex systems but also affirmed existing research pertaining to the identification of attractors and repellors. More importantly, however, this research expanded the definition and articulation of the behaviors of attractors and repellors. This research more clearly identified and articulated these behaviors in the context of classroom dialogs that occurred over the duration of a semester long course. Further, this research demonstrated the development of additional methods for identifying and evaluating complex systems qualitatively.
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Schirtz, Tom. "Etude du mécanisme de translocation de l'ARNtLys dans les mitochondries de Saccharomyces cerevisiae." Phd thesis, Université de Strasbourg, 2012. http://tel.archives-ouvertes.fr/tel-00868796.
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L'import d'ARN dans les mitochondries est un processus ubiquitaire chez les eucaryotes. Dans Saccharomyces cerevisiae un isoaccepteur cytosolique de l'ARNtLys, l'ARNtLys(CUU) (tRK1), est partiellement importé dans les mitochondries. L'adressage vers la surface mitochondriale a été étudié en détail mais l'étape de translocation dans les mitochondries reste toujours à démontrer. L'objectif principal de ce travail de thèse était l'identification et la caractérisation des protéines qui participent dans ce processus. Deux protéines de la membranes externe capables de former des canaux, Tom40 et VDAC1, ont été identifiées et leur rôle dans l'étape de translocation a été évalué in vitro et in vivo en utilisant des souches mutantes appropriées ou des agents capables de bloquer de manière spécifique les canaux formés par les protéines Tom40 et VDAC1. Ainsi il a été démontré que la délétion de VDAC1 ou l'inhibition du canal VDAC1 a conduit à une inhibition importante, néanmoins pas complète, de l'import de tRK1. Le blocage simultané des canaux Tom40 et VDAC1 par contre a causé un arrêt complet de l'import de tRK1 in vitro. Vu ces résultats, nous proposons que la translocation de tRK1 à travers la membrane mitochondriale externe puisse suivre deux chemins alternatifs.
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Holmes, Peter. "Structure and mode of action of the TolA-TolB complex from Pseudomonas aeruginosa." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:cccb0c88-5c89-4d21-81eb-70ebf513c7ab.
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Protein-protein interactions (PPIs) across the cell envelope of Gram-negative bacteria are critical for mediating signal transduction pathways that underpin cellular homeostasis. The Ton and Tol Pal systems are two conserved, ancestrally related protein networks that are also required for bacterial pathogenesis. Both Ton and Tol-Pal traverse the periplasm to effect different functions at the outer membrane (OM). Tol-Pal is composed of a homologous complex of three inner membrane proteins, TolQ-TolR-TolA (linked to proton motive force) and two additional periplasmic proteins TolB and Pal. The physiological role of the Tol-Pal system is to stabilise the OM, however the mechanism involved is unknown. TolA is however known to form a crucial protein-protein interaction via its C-terminus with the disordered N-terminus of TolB. Prior to this thesis, determination of the molecular features underlying a protein-protein complex between TolA and an endogenous binding partner TolB had never been accomplished. In this work, I describe the first structure comprising the TolA-TolB complex from Gram negative bacteria. The structure of this complex was determined from Pseudomonas aeruginosa by solution NMR spectroscopy. I determined the interaction between P. aeruginosa TolA and a TolB N terminal peptide to be relatively weak using fluorescence anisotropy. I found that TolB interacts with TolA through an analogous mechanism to that seen in TonB-dependent transporters. Based on these studies and bioinformatics analyses, I hypothesize that the evolutionary resilience of the Tol-Pal system to external pressures is contingent on the preservation of the TolA-TolB interface. Structure-based mutations within the TolA-TolB complex were also evaluated for their effect on in vivo function of the Tol-Pal complex and impact on complex formation in vitro. Taken together, the results demonstrate that protein networks which transduce energy to the OM through PMF-dependent systems in bacterial cells appear to follow a common β-strand augmentation mechanism.
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Zimmermann, Mathieu. "Complexes de chaînes et petites catégories." Université Louis Pasteur (Strasbourg) (1971-2008), 2004. http://www.theses.fr/2004STR13044.
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Cette thèse a pour thème l'étude homologique de modules sur certaines petites catégories et se divise en trois parties. La première partie donne un théorème de changement de base pour les foncteurs Tor. Celui-ci trouve un champ d'applications dans ce qu'on définit comme étant des catégories croisées. Ce formalisme nous permet de réinterpréter un théorème de Pirashvili et Richter sur l'homologie cyclique et l'homologie de Hochschild. La deuxième partie utilise le théorème de changement de base pour expliciter une relation entre la Gamma-homologie et l'homologie de Lie. Pour cela, on définit deux catégories Leib et Li, associées aux algèbres de Leibniz et de Lie, permettant d'étendre les théories d'homologies usuelles à une classe plus large d'objets. La structure de catégorie croisée de Li permet de construire un bicomplexe calculant la Li-homologie. La troisième partie a pour but de donner une présentation par générateurs et relations de la catégorie annulaire. Cette catégorie est définie par les opérations unaires de l'opérade planaire introduite par V. Jones et contient deux copies de la catégorie cyclique.
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Moreno, Villavicencio Maiglid Andreina. "Development of 3D high-resolution imaging of complex devices by the correlation of ToF-SIMS and AFM." Thesis, Lyon, 2019. http://www.theses.fr/2019LYSEI122.
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La miniaturisation continue et la complexité des dispositifs poussent les techniques existantes de nano-caractérisation à leurs limites. De ce fait, la combinaison de ces techniques apparait être une solution attrayante pour continuer à fournir une caractérisation précise et exacte. Dans le but de dépasser les verrous existants pour l’imagerie chimique 3D haute résolution à l’échelle nanométrique, nous avons concentré nos recherches sur la création d’un protocole combinant la spectrométrie de masse à ions secondaires de temps de vol (ToF-SIMS) avec la microscopie à force atomique (AFM). Ceci permet entre autre de corréler la composition et la visualisation en 3 dimensions avec des cartographies de topographie ou d’autres propriétés locales fournies par l’AFM. Trois principaux résultats sont obtenus grâce à cette méthodologie : la correction d’un ensemble de données ToF-SIMS pour une visualisation 3D sans artefacts, la cartographie du taux de pulvérisation locale permettant de mettre en évidence les effets liés à la rugosité et la présence d’interfaces verticales et la superposition des informations avancées ToF-SIMS et AFM. Quatre applications de la méthodologie combinée ToF-SIMS et AFM sont abordées dans cette thèse. La procédure de correction des données ToF-SIMS en 3D a été appliquée sur une structure hétérogène GaAs / SiO2. Les artefacts liés à la pulvérisation, notamment l’effet d’ombrage, ont été étudiés par le biais des cartographies de taux de pulvérisation sur des échantillons avec nano-motifs structurés et non structuré. Enfin, nous avons exploré la combinaison de l’analyse ToF-SIMS avec trois modes avancées de microscopie AFM : piézoélectrique (PFM), capacité (SCM) et conducteur (SSRM). Une première étude a notamment permis d’observer l’évolution et la modification chimique suite à l’application d’une contrainte électrique sur deux film mince piézoélectriques. Une deuxième étude s’est focalisée sur l’impact de l’implantation Ga lors de la préparation d’échantillons par FIB pour voir comment limiter l’effet de l’amorphisation sur la mesure électrique. Les aspects techniques de la méthodologie seront abordés pour chacune de ces applications et les perspectives de cette combinaison seront discutésThe continuous miniaturization and complexity of devices have pushed existing nano-characterization techniques to their limits. The correlation of techniques has then become an attractive solution to keep providing precise and accurate characterization. With the aim of overcoming the existing barriers for the 3D high-resolution imaging at the nanoscale, we have focused our research on creating a protocol to combine time-of-flight secondary ion mass spectrometry (ToF-SIMS) with atomic force microscopy (AFM). This combination permits the correlation of the composition in 3-dimensions with the maps of topography and other local properties provided by the AFM. Three main results are achieved through this methodology: a topography-corrected 3D ToF-SIMS data set, maps of local sputter rate where the effect of roughness and vertical interfaces are seen and overlays of the ToF-SIMS and AFM advanced information. The application fields of the ToF-SIMS and AFM combined methodology can be larger than expected. Indeed, four different applications are discussed in this thesis. The procedure to obtain the topography-corrected 3D data sets was applied on a GaAs / SiO2 patterned structure whose initial topography and composition with materials of different sputter rates create a distortion in the classical 3D chemical visualization. The protocol to generate sputter rate maps was used on samples with structured and non-structured nano-areas in order to study the possible ToF-SIMS sputtering artefacts, especially the geometric shadowing effect. Finally, we have explored the combination of ToF-SIMS analysis with three AFM advanced modes: piezoresponse force microcopy (PFM), scanning capacitance microscopy (SCM) and scanning spreading resistance microscopy (SSRM). Specifically, two main applications were studied: the chemical modification during electrical stress of a piezoelectic thin film and the recovery of initial electrical characteristics of a sample subjected to Ga implantation during FIB preparation. Technical aspects of the methodology will be discussed for each application and the perspectives of this combination will be given
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Ham, Bryan Melvin. "Development of mass spectrometric methods for the analysis of components and complex interactions in biological systems." ScholarWorks@UNO, 2005. http://louisdl.louislibraries.org/u?/NOD,237.
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Thesis (Ph. D.)--University of New Orleans, 2005.Title from electronic submission form. "A dissertation ... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Chemistry"--Dissertation t.p. Vita. Includes bibliographical references.
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Klein, Astrid. "A structural analysis of the TOB complex, the insertase for Beta-barrel proteins of the mitochondrial outer membrane." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-150786.
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Sá, Cristiane Freire de. "A açãode tutoria em ambientes virtuais de aprendizagem na Rede e-Tec Brasil: uma visão complexa." Pontifícia Universidade Católica de São Paulo, 2015. https://tede2.pucsp.br/handle/handle/13745.
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Previous issue date: 2015-07-27Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
This research aims to describe and interpret a phenomenon of human experience: tutoring action developed by virtual tutors teachers in virtual learning environments in he context of the educational network called Rede E-Tec Brazil; so trying to understand, under the bias of the complexity presented by Morin, the manifestations of this phenomenon from the subjects who experience it, in the case, virtual tutors. Mentoring is a word in the context of distance education which has named the educational activity that teachers spend developing in virtual environments and has been the subject of studies and discussions in Brazil, from different perspectives, especially as a variation of the traditional teaching activities . However, most studies focus on this theme in the context of higher education, labor relations or responsibilities and regulations involving this form of mediating student learning in virtual environments. In order to contribute from the specifics of Professional Technical Education and the perspective of those who perform the tutoring action in this mode, this research aims to discuss the fragmentation of pedagogical action and hence the division of teaching involving the transformation of autonomy and profession. Developed within the framework of contemporary Applied Linguistics, aimed to investigate experiences and social practices revealed through language, this study takes as its starting point the reports, interpretations and reflections of those who live virtual mentoring, a teaching modality that has cultures and particular identities. From the reality experienced by the subject virtual tutor, we seek to articulate an interpretation of experiences in virtual learning environments, materialized by language, with studies on virtual teaching and studies about virtual mentoring, linking them with the theoretical vision of teaching. Drawing up as a qualitative-interpretative research, this work is methodologically oriented by Hermeneutic-phenomenological approach and complexity that allows the description and interpretation of human experience considering also the theoretical contributions of hermeneutics and phenomenology in the light of complexity by Morin. This methodological approach has allowed to develop research in the field of Applied Linguistics, considering language as revealing interface that embodies the experiences that are textualized under the hermeneutic phenomenological bias. The collection of textualized reports from the five tutors, the essence of mentoring phenomenon action in virtual environments, object of the present study, is revealed from the hermeneutic-phenomenological themes: challenges; difficulties, questions; concerns and relationships that present themselves as relevant to think about the teachers of distance education, especially within the technical vocational education
A presente pesquisa objetiva descrever e interpretar um fenômeno da experiência humana: a ação de tutoria desenvolvida pelos professores-tutores virtuais em ambientes virtuais de aprendizagem no contexto da Rede e-Tec Brasil; dessa forma, buscando compreender, sob o viés da complexidade apresentada por Morin, as manifestações desse fenômeno a partir dos sujeitos que o vivenciam, no caso, os tutores virtuais. A palavra tutoria, no contexto da educação a distância, tem nomeado a ação educativa que os sujeitos professores passam a desenvolver nos ambientes virtuais e tem sido tema de estudos e discussões no Brasil, a partir de diversas perspectivas, especialmente como uma variação das atividades da docência tradicional. Entretanto, a maioria dos estudos foca essa temática no contexto do Ensino Superior ou no das relações trabalhistas ou das responsabilidades e prescrições que envolvem essa forma de mediar a aprendizagem dos alunos nos ambientes virtuais. Visando contribuir, a partir das especificidades do Ensino Técnico-Profissionalizante e pela perspectiva dos sujeitos que realizam a ação de tutoria nessa modalidade, essa pesquisa pretende problematizar a fragmentação da ação pedagógica e, consequentemente, a divisão do trabalho docente, que implicam nas transformações da autonomia e da profissão. Desenvolvendo-se no âmbito da Linguística Aplicada contemporânea, que possibilita investigar experiências e práticas sociais reveladas por meio da linguagem, este estudo tem, como ponto de partida, os relatos, as interpretações e as reflexões de quem vive a tutoria virtual numa modalidade de ensino que possui culturas e identidades particulares. Ao partir da realidade vivida pelo sujeito tutor virtual, busca-se articular uma interpretação das experiências vividas em ambientes virtuais de aprendizagem, materializadas pela linguagem, com os estudos sobre a docência virtual e com os estudos acerca da tutoria virtual, articulando-os com a visão teórica de docência. Desenhando-se como uma pesquisa de natureza qualitativa-interpretativa, este trabalho tem, como orientação metodológica, a Abordagem Hermenêutico-Fenomenológica e Complexidade que possibilita a descrição e a interpretação das experiências humanas considerando, igualmente, os aportes teóricos da hermenêutica e da fenomenologia, à luz da complexidade de Morin. Essa abordagem metodológica tem permitido desenvolver pesquisa no âmbito da Linguística Aplicada, tendo a linguagem como interface reveladora que corporifica as experiências vividas que são textualizadas sob o viés hermenêutico-fenomenológico. A partir da coleta de textos oriundos dos relatos de cinco tutoras, a essência do fenômeno ação de tutoria em ambientes virtuais, objeto do presente estudo, se revela a partir dos temas hermenêutico-fenomenológicos: desafios; dificuldades, questionamentos; preocupações e relações que se apresentam como relevantes para pensar os sujeitos professores da educação a distância, especialmente no âmbito da educação profissional técnica
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Amamura, Thaís Akemi. "Clivagem de proteínas do complexo de ataque à membrana do sistema complemento humano por proteases de leptospiras patogênicas." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-09032017-134114/.
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A leptospirose é causada por bactérias que pertencem ao gênero Leptospira. Em um estudo realizado por nosso grupo, observou-se que as proteases secretadas por leptospiras patogênicas foram capazes de clivar a molécula C3 do Complemento e seus fragmentos C3b e iC3b, além de Fator B, C4b e C2. Neste trabalho expandimos a análise da atividade proteolítica sobre os componentes do Complexo de Ataque à Membrana (MAC): C6, C7, C8 e C9. Nós observamos que essas proteases clivam todos os componentes do MAC inclusive o complexo solúvel formado e que essas clivagens ocorrem de modo tempo-dependente. Além disso, as clivagens dessas moléculas ocorrem de modo seletivo, pois mesmo utilizando quantidades reduzidas de sobrenadantes ainda foi possível observar produtos de clivagem. A atividade proteolítica foi inibida pela 1,10fenantrolina, indicando a participação de metaloproteases. O reconhecimento de quais moléculas do MAC são clivadas por proteases de leptospiras patogênicas pode contribuir para o desenvolvimento de estratégias terapêuticas na infecção por estes patógenos.Leptospirosis is a zoonosis caused by spirochetes from the genus Leptospira. In a previous study, our group observed that the proteases secreted by Pathogenic Leptospira were capable of cleaving C3 of Complement, as well as the fragments C3b and iC3b, Factor B (Alternative Pathway), C4 and C2 (Classical and Lectin Pathways). In this work, we analyze the activity of the leptospiral proteases on the components of Membrane Attack Complex (MAC). We observed that the protease cleaves all MAC components including soluble complex formed and that these cleavages occur in a time-dependent manner and in a selective way, since even when reduced quantities of supernatants were used, the cleavage products were still observed. The proteolytic activity was inhibited by 1,10phenanthroline, indicating the participation of metalloproteinases. The recognition that MAC molecules are cleaved by proteases of pathogenic leptospires can contribute to the development of therapeutic strategies for the infection by these pathogens.
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Gillani, Syed. "Semantically-enabled stream processing and complex event processing over RDF graph streams." Thesis, Lyon, 2016. http://www.theses.fr/2016LYSES055/document.
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Résumé en français non fourni par l'auteurThere is a paradigm shift in the nature and processing means of today’s data: data are used to being mostly static and stored in large databases to be queried. Today, with the advent of new applications and means of collecting data, most applications on the Web and in enterprises produce data in a continuous manner under the form of streams. Thus, the users of these applications expect to process a large volume of data with fresh low latency results. This has resulted in the introduction of Data Stream Processing Systems (DSMSs) and a Complex Event Processing (CEP) paradigm – both with distinctive aims: DSMSs are mostly employed to process traditional query operators (mostly stateless), while CEP systems focus on temporal pattern matching (stateful operators) to detect changes in the data that can be thought of as events. In the past decade or so, a number of scalable and performance intensive DSMSs and CEP systems have been proposed. Most of them, however, are based on the relational data models – which begs the question for the support of heterogeneous data sources, i.e., variety of the data. Work in RDF stream processing (RSP) systems partly addresses the challenge of variety by promoting the RDF data model. Nonetheless, challenges like volume and velocity are overlooked by existing approaches. These challenges require customised optimisations which consider RDF as a first class citizen and scale the processof continuous graph pattern matching. To gain insights into these problems, this thesis focuses on developing scalable RDF graph stream processing, and semantically-enabled CEP systems (i.e., Semantic Complex Event Processing, SCEP). In addition to our optimised algorithmic and data structure methodologies, we also contribute to the design of a new query language for SCEP. Our contributions in these two fields are as follows: • RDF Graph Stream Processing. We first propose an RDF graph stream model, where each data item/event within streams is comprised of an RDF graph (a set of RDF triples). Second, we implement customised indexing techniques and data structures to continuously process RDF graph streams in an incremental manner. • Semantic Complex Event Processing. We extend the idea of RDF graph stream processing to enable SCEP over such RDF graph streams, i.e., temporalpattern matching. Our first contribution in this context is to provide a new querylanguage that encompasses the RDF graph stream model and employs a set of expressive temporal operators such as sequencing, kleene-+, negation, optional,conjunction, disjunction and event selection strategies. Based on this, we implement a scalable system that employs a non-deterministic finite automata model to evaluate these operators in an optimised manner. We leverage techniques from diverse fields, such as relational query optimisations, incremental query processing, sensor and social networks in order to solve real-world problems. We have applied our proposed techniques to a wide range of real-world and synthetic datasets to extract the knowledge from RDF structured data in motion. Our experimental evaluations confirm our theoretical insights, and demonstrate the viability of our proposed methods
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Barisic, Valeria. "Characterization of Putative ExbB and ExbD Leads to the Identification of a Potential Tol-Pal System in Rhizobium leguminosarum ATCC 14479." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etd/2489.
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Rhizobium leguminosarum is a Gram negative nitrogen-fixing soil bacterium. Due to the limited bioavailability of iron, bacteria utilize siderophores that scavenge and bind available iron. The transport of iron-siderophore complexes is achieved by the TonB-ExbB-ExbD complex. We have previously shown that a functional TonB protein is necessary for iron transport by creating ΔtonB mutants and assessing their growth and 55Fe-siderophore transport ability. We attempted to identify and characterize the roles of putative exbB and exbD genes using a similar approach. Growth curves and sequence analyses suggest putative exbB and exbD may be the tolpal-associated genes tolQ and tolR. Phenotypic and sensitivity assays showed mutants do not exhibit the characteristic tol phenotype and are not sensitive to detergents or changes in ionic strength of the growth medium. We also expressed and purified the 120 amino acid fragment of the TonB C-terminus for further physical and chemical characterization.
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Shafeie, Samrand. "Properties in New Complex Perovskite-Related Materials, a Matter of Composition and Structure." Doctoral thesis, Stockholms universitet, Institutionen för material- och miljökemi (MMK), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-88793.
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This PhD thesis presents investigations of perovskite-related compounds in systems of interest for applications in components in solid oxide fuel cells. The compound compositions derive from substitutions in the parent compounds LaCoO3, LaCrO3 and SrFeO3. Novel phases La2Co1+z(MgxTi1-x)1-zO6 were synthesized and investigated with regard to structure, thermal expansion, electronic and magnetic properties. The study focused on the composition lines La2Co(MgxTi1-x)O6 (z=0), where the oxidation state of Co nominally changes from +2 (x=0.0) to +3 (x=0.5), and La2Co1+z(Mg0.5Ti0.5)1-zO6, with a varying fraction of Co3+ ions. XANES data show that the Co ions in the system have discrete oxidation states of +2 and +3. The TEC increases with increasing x due to an increasing contribution from spin state transitions of the Co3+ ions. Novel compounds La2Cr(M2/3Nb1/3)O6 with M=Mg, Ni, Cu were synthesized and characterized with respect to structure and magnetic properties. XRPD and NPD data indicate Pbnm symmetry; however, SAED patterns and HREM images indicate a P21/n symmetry for M=Mg, and Cu. The magnetic measurements results were rationalized using the Goodenough-Kanamori rules. Oxygen-deficient phases with x≥0.63 in SrxY1-xFeO3-δ and Sr0.75Y0.25Fe1-yMyO3-δ (M=Cr, Mn, Ni and y=0.2, 0.33, 0.5), were synthesized and characterized with respect to structure, oxygen content, thermogravimetry, TEC, conductivity and magnetic properties. Powder patterns of phases agree with cubic perovskite structures. NPD data for x=0.75 reveal anisotropic displacement for the O atom, related to local effects from Fe3+/Fe4+ ions. SAED patterns for x=0.75 reveal the presence of an incommensurate modulation. The compounds start to lose oxygen in air at ~ 400°C. The TEC up to ~400°C for x=0.75 is ~10.5 ppm/K and increase to ~17.5 ppm/K at higher temperatures. The conductivity for x=0.91 is 164 S/cm at 400°C. Partial substitution of Fe by Cr, Mn or Ni does not increase the conductivity or decrease TEC.
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Goemaere, Emilie. "Le complexe membranaire Tol d'Escherichia coli : un modèle pour l'étude de l'organisation fonctionnelle d'un moteur moléculaire bactérien." Aix-Marseille 2, 2008. http://theses.univ-amu.fr.lama.univ-amu.fr/2008AIX22032.pdf.
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Le système protéique Tol-Pal est conservé chez les bactéries à Gram négatif. Chaque mutant tol ou pal provoque une déstabilisation de la membrane externe (ME). Les protéines TolA-Q-R forment un complexe de membrane interne (MI) ; Pal ancrée dans la ME interagit avec le peptidoglycane et la protéine périplasmique TolB. L’interaction entre TolA et Pal dépend de TolQ-R et de la force proton-motrice (fpm). Les protéines TolA-Q-R relient ainsi MI, ME et le peptidoglycane. Le but de ce projet est de comprendre l’organisation des segments transmembranaires (sTM) de TolQ-R et le mécanisme énergétique. Les interactions entre sTM ont été analysées ainsi que les résidus impliqués dans le passage d’ions. Nous avons montré que le domaine C-terminal de TolR est dynamique en réponse à la fpm. Nous avons entrepris une mutagénèse systématique de chacun des résidus des sTM. Les résultats obtenus ont permis de dresser un modèle dynamique de l’organisation des sTM du complexe TolQ-RThe Tol-Pal system is well conserved in Gram negative bacteria. Each of the tol- pal mutant exhibits outer membrane (OM) defects. The TolQ-R-A proteins form an inner membrane (IM) complex; Pal anchored in the OM interacts with the peptidoglycan and the periplasmic protein TolB. The TolA-Pal interaction depends on TolQ-R and on the proton motive force (pmf). The TolQ-R-A proteins may form a molecular motor using the pmf to link IM, OM and the peptidoglycan. The aim of this work is to understand the organisation of the IM TolQ-R proteins and the utilisation of pmf. Using mutagenesis approaches, we defined helix organization and key residues of the putative ion channel. We demonstrated movements of the C-terminus of TolR dependent on the pmf. To define complex organisation and conformational changes of the transmembrane segments (TMs), we performed a cysteine scanning mutagenesis. Our results suggest a dynamic model of the TolQ-R TMs organisation
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Ingram, Niamh. ""Too complex for IAPT" : service-user experiences of accessing specialist services and the process of being diagnosed with borderline personality disorder." Thesis, University of Surrey, 2018. http://epubs.surrey.ac.uk/849499/.
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Background: There have been significant advances in research, treatment, policy and service provision for people diagnosed with a personality disorder in England. However, diagnoses such as borderline personality disorder (BPD), continue to be controversial due to the validity of the construct itself, the ability of clinicians to diagnose reliably, and the significant impact stigma can have on those diagnosed. Aims: This study aims to provide a current service-user perspective on the diagnostic process in BPD within specialist PD outpatient services. It specifically focuses on how service-users perceive this process to influence how they understand themselves, given that past research has indicated a BPD diagnosis can influence self-concept, and understanding of their mental health, given research has highlighted a lack of clarity from professionals about the diagnosis. Method: Data were collected from six in-depth interviews with people currently accessing complex needs services, within a London NHS trust, who had experienced the diagnostic process in BPD. Transcripts were analysed using interpretative phenomenological analysis (IPA). Results: Two superordinate themes emerged from the data: “Making sense of a BPD diagnosis” which encapsulated participants’ experiences of understanding the diagnosis, in terms of whether it “fitted” from their perspective, how it made sense of traumatic and challenging past experiences and the clarity they received from professionals; “Struggling to get what I need from services” which captures participants’ experiences of needing to reach crisis point and exhaust all other treatment options before accessing specialist assessment and treatment. Conclusions: Service-users continue to experience a lack of clarity during the diagnostic process in BPD, particularly within secondary care, and feel they struggle to access the specialist support they need. The diagnostic process is an opportunity to help service-users make sense of their current difficulties and past experiences, and to help them feel fully informed about treatment choices.
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Dobrenel, Thomas. "Etude de la voie de signalisation et du complexe TOR (Target Of Rapamycin) chez Arabidopsis." Phd thesis, Université Paris Sud - Paris XI, 2012. http://tel.archives-ouvertes.fr/tel-00880882.
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La protéine kinase TOR (Target Of Rapamycin) a été identifiée chez la levure et les mammifères comme participant à deux complexes protéiques qui servent de carrefour entre la perception des facteurs endogènes et exogènes et la stimulation de la croissance cellulaire. Depuis la découverte de la kinase AtTOR chez Arabidopsis thaliana, des études ont été menées afin de mieux caractériser son rôle chez les plantes et l'influence de son niveau d'expression sur la régulation du métabolisme et du développement.Au cours de ce travail, j'ai contribué à l'étude de cette kinase en étudiant l'influence de l'inactivation de TOR sur la composition du ribosome au niveau protéique et sur le niveau de phosphorylation de ces protéines, ainsi que sur l'organisation du méristème au niveau moléculaire et cytologique Au cours de cette étude, j'ai montré que certaines protéines constitutives du ribosome pourraient être des cibles de l'activité TOR au niveau de leur abondance et/ou de leur état de phosphorylation. Ainsi, l'inactivation de TOR entraine une diminution du niveau de phosphorylation des protéines RPS6 et pourrait influencer l'abondance des protéines acides constitutives du stalk ribosomal, une structure importante dans la régulation de la traduction. Les résultats obtenus suggèrent également que l'activité TOR est nécessaire au maintien du méristème à l'état fonctionnel en régulant les voies importantes contrôlant la division et la différentiation au sein de cette structure.
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Farkas, Maia Jivkova. "Multi-Task Setting Involving Simple and Complex Tasks: An Exploratory Study of Employee Motivation." Scholar Commons, 2014. http://scholarcommons.usf.edu/etd/4887.
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In this study, employees are given autonomy in effort allocation across two tasks - complex and simple tasks, where the return to the organization is significantly higher for the complex task requiring high skill than for the simple task requiring low skill. An unavoidable feature of multi-task settings is that effort expended on one task detracts from effort that can be expended on another task. This effort trade-off among tasks becomes problematic when the returns from different tasks are unequal, with important consequences for a firm's overall performance. The design of management accounting control systems in such multi-task setting is difficult because organizations have to achieve multiple objectives: to improve productivity on both simple and complex tasks (i.e., performance) and to direct employee effort to more complex tasks given that the complex tasks are more valuable to firms (i.e., effort allocation). In a laboratory experiment, I examine the effects of two motivational mechanisms, financial compensation and relative performance information (RPI), on employee performance and effort allocation between simple and complex tasks. I find that the effects of RPI and financial compensation are independent such that each motivational mechanism affects performance and effort allocation separately. In addition, I find that the effects of RPI or financial compensation depend on whether a worker is a top performer or a bottom performer. Also, findings demonstrate that the effects of these motivational mechanisms on employee effort allocation and performance depend on the complexity of the task. Future research studies and managers who design incentive systems should consider the implementation of different types of incentives for different performer levels. Organizations should consider the degree of complexity of the tasks that workers must perform in multi-task settings.
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Pereira, Vitor Emanuel M. Loureiro S. "Computer model to predict electron beam-physical vapour deposition (EB-PVD) and thermal barrier coating (TBC) deposition on substrates with complex geometry." Thesis, Cranfield University, 2000. http://dspace.lib.cranfield.ac.uk/handle/1826/5714.
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For many decades gas turbine engineers have investigated methods to improve engine efficiency further. These methods include advances in the composition and processing of materials, intricate cooling techniques, and the use of protective coatings. Thermal barrier coatings (TBCs) are the most promising development in superalloy coatings research in recent years with the potential to reduce metal surface temperature, or increase turbine entry temperature, by 70-200°C. In order for TBCs to be exploited to their full potential, they need to be applied to the most demanding of stationary and rotating components, such as first stage blades and vanes. Comprehensive reviews of coating processes indicate that this can only be achieved on rotating components by depositing a strain-tolerant layer applied by the electron beam-physical vapour deposition (EB-PVD) coating process. A computer program has been developed in Visual c++ based on the Knudsen cosine law and aimed at calculating the coating thickness distribution around any component, but typically turbine blades. This should permit the controlled deposition to tailor the TBC performance and durability. Various evaporation characteristics have been accommodated by developing a generalised point source evaporation model that involves real and virtual sources. Substrates with complex geometry can be modelled by generating an STL file from a CAD package with the geometric information of the component, which may include shadow-masks. Visualisation of the coated thickness distributions around components was achieved using OpenGL library functions within the computer model. This study then proceeded to verify the computer model by first measuring the coating thickness for experimental trial runs and then comparing the calculated coating thickness to that measured using a laboratory coater. Predicted thickness distributions are in good agreement even for the simplified evaporation model, but can be improved further by increasing the complexity of the source model.
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Richerd, Mathilde. "Développement d'un système de microfluidique de gouttes pour l'analyse d'échantillons complexes." Thesis, Université Paris sciences et lettres, 2021. http://www.theses.fr/2021UPSLS051.
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La microfluidique de gouttes est une technologie qui possède un très grand potentiel pour la miniaturisation et l’automatisation des méthodes bioanalytiques conventionnelles. En effet, les nombreuses fonctionnalités présentes en microfluidique de gouttes, telles que la fusion, le tri et l’encapsulation de cellules, permettent de reproduire des protocoles bioanalytiques standards sur de très petits volumes avec des avantages tels que la diminution de la consommation d’échantillon et de réactifs et la diminution du temps d’analyse. Au cours de cette thèse, nous avons développé les protocoles pour deux types d’analyse biologique utilisant la manipulation de particules magnétiques en gouttes de 100 nL.Le premier projet a porté sur l’analyse multiomique de cellules uniques. Nous avons conçu un protocole en microfluidique de gouttes permettant la séparation de l’ARNm et l’ADN d’un échantillon complexe : du mélange d’ADN et d’ARN pré-purifiés à une suspension de moins d’une dizaine de cellules entières. A chaque étape, les performances du système en gouttes ont été évaluées et les résultats comparés avec les extractions effectuées en tubes de manière non-microfluidique. Les résultats obtenus sont prometteurs car ils démontrent pour la première fois dans un tel dispositif la séparation séquentielle de l’ADN et de l’ARNm à partir de lysat cellulaire ou de quelques cellules entières encapsulées et lysées en gouttes.Le second projet concerne la préparation d’échantillon en gouttes pour l’analyse par spectrométrie de masse MALDI-TOF. Ce projet a été mené en collaboration avec des chercheurs du CEA Saclay qui ont montré qu’il y a un intérêt important à déposer les échantillons sur des plaques MALDI sous forme de gouttes pour augmenter la sensibilité de détection grâce à un effet de concentration. Nous avons mis en place un système automatisable qui permet de coupler la microfluidique de gouttes à la spectrométrie de masse MALDI-TOF : de la digestion enzymatique supportée en goutte au dépôt sur plaque avec pré-concentration de l’échantillon cristallisé. Le développement a été fait avec le lysozyme comme protéine modèle et notre protocole permet d’identifier la protéine par empreinte de masses peptidiques à partir de 200 fmol de protéine avec une bonne fiabilitéDroplet microfluidics is a technology with a huge potential for miniaturization and automation of conventional methods in bioanalysis. Indeed, droplet microfluidics has many functionalities, such as merging, sorting and cell encapsulation, that can reproduce manipulations in standard protocols in bioanalysis on very small volumes with advantages such as a low samples and reagents consumption and reduction of analysis duration. During this thesis, we developed protocols for two types of biological analysis, using magnetic particles manipulation in 100 nL droplets.The first project is about single cell multiomics analysis. We implemented a droplet microfluidics protocol for the separation of mRNA and DNA from complex samples: from a mix of pre-purified RNA and DNA to a suspension of less than ten cells. At each steps, the droplet system performances were evaluated and the results were compared to extractions made in tubes in a non-microfluidics way. The results are very promising since they demonstrate for the first time in such a system the sequential separation of DNA and RNA from cell lysate or a few cells encapsulated and lysed in droplets.The second project is about the sample preparation in droplet for MALDI-TOF mass spectrometry analysis. This project was built in collaboration researchers from CEA Saclay. They have shown that it is very interesting to deposit samples in droplets on MALDI plates to increase the detection sensitivity thanks to a focusing effect. We have implemented on an original integrated droplet microfluidic approach for protein analysis by MALDI-TOF MS: from in drop sample preparation by supported enzymatic digestion to on plate deposition and peptides local pre-concentration. Development was done with lysozyme as a model protein and thanks to our in-drop digestion and deposition protocol we identified this protein by mass fingerprint from 200 fmol protein with a good reliability
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Madaan, Nitesh. "Synthesis and Characterization of Complex Molecular Assemblies on Surfaces." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/5748.
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The research presented in this dissertation is focused on the construction of complex molecular structures on planar gold and silicon dioxide surfaces using a variety of surface modification techniques, along with thorough surface characterization at each modification step. The dissertation is structured into six separate chapters. In Chapter 1, an introduction to the importance and implications of molecular level surface modification, commonly employed surface modification methods, and available surface characterization techniques is presented. Chapter 2 shows applications of novel methodologies for the functionalization of gold surfaces using alkane dithiol self-assembled monolayers and thiol-ene click chemistry. The resulting functionalized gold substrates demonstrate higher chemical stability than alkanethiol self-assembled monolayers alone and allow spatially controlled functionalization of gold surfaces with light. In Chapter 3, work on tunable hydrophobic surfaces is presented. These surfaces are prepared using a combination of organosilane chemistry, layer-by-layer polyelectrolyte deposition, and thiol-ene chemistry. These hydrophobic surfaces demonstrate high mechanical and chemical stability, even at low pH (1.68). The pinning of water droplets could be tuned on them by the extent of their thermal treatment. Comprehensive surface characterization using X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectrometry (ToF-SIMS), spectroscopic ellipsometry, atomic force microscopy, and water contact angles was carried out on the molecular assemblies prepared on gold and silicon dioxide surfaces. Chapters 4 and 5 are focused on the application, data interpretation, and enhancement in sensitivity of different surface characterization methods. In Chapter 4, XPS, ToF-SIMS, and principal components analysis are used to probe a real world corrosion-type problem. This systemic study showed the destruction of a protective coating composed of a nitrilotris(methylene)triphosphonic acid by a low-intensity fluorine plasma. In Chapter 5, enhancement in ToF-SIMS signals is shown via bismuth metal deposition. These surfaces are also probed by spectroscopic ellipsometry using the interference enhancement method. Finally, Chapter 6 concludes this dissertation by describing possible future work.
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Klein, Astrid [Verfasser], and Angelika [Akademischer Betreuer] Böttger. "A structural analysis of the TOB complex, the insertase for beta-barrel proteins of the mitochondrial outer membrane / Astrid Klein. Betreuer: Angelika Böttger." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2011. http://d-nb.info/1028490461/34.
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Wylezich, Thomas Lukas [Verfasser], Nathalie [Akademischer Betreuer] Kunkel, Nathalie [Gutachter] Kunkel, Tom [Gutachter] Nilges, and Philippe [Gutachter] Goldner. "Luminescence of Lanthanide Doped Mixed Anionic Hydrides and Complex Hydrides / Thomas Lukas Wylezich ; Gutachter: Nathalie Kunkel, Tom Nilges, Philippe Goldner ; Betreuer: Nathalie Kunkel." München : Universitätsbibliothek der TU München, 2020. http://d-nb.info/1220321362/34.
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Jean, Mireille. "La chambre des comptes de Lille : (1477 - 1667) ; l'institution et les hommes /." Paris : École des Chartes, 1992. http://www.gbv.de/dms/spk/sbb/recht/toc/275770745.pdf.
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DIAS, Tania Regina Frota Vasconcellos. "Compet?ncias empreendedoras e focos comportamentais: estudo de casos dos empreendedores das empresas ganhadoras do pr?mio Top Empresarial 2006 e 2007." Universidade Federal Rural do Rio de Janeiro, 2009. https://tede.ufrrj.br/jspui/handle/tede/1014.
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Previous issue date: 2009-04-27In the last years, it was consolidated the importance that micro and small business represent in the economic and social setting of the country. At the same time, it is incontrovertible that the competitiveness is essential to the inclusion of Brazilian companies in markets more and more globalized. In that context, the interest in entrepreneurship grows. Entrepreneurial competences take the lead role. The aim of this study is to identify how such entrepreneurial competences take part in the history of the life of the agents of the winning companies of the 2006 and 2007 Corporate TOP Awards and what made them successful people. This research has, as basis, the taxonomy presented by Vergara (2006): as far as to the ends, exploratory and descriptive and, to the means, bibliographic, documental, and multiple cases studies. The data were gathered through a semi-structured questionnaire and sent to the awarded people, after they were personally contacted. The treatment and the data analysis were developed through the Method of Content Analysis, mixed categorical analysis where they were defined, a priori, based on the theory of Man and Lau (2000). The sample of the first study, related to the winners of the 2006 Corporate TOP Award, had five out of six entrepreneurs; and, the sample of the second study, related to the winners of the 2007 Corporate TOP Award, had six out of eight. Seven entrepreneurial competences area stood out in the study. The results show that three of the seven competences area considered relevant and common: Conceptual Competence, Strategic Competence and Support Competence; and, they represent 58.77% of the related frequency, emphasizing the first and the second ones that stand for 24.85% of the total. It can be inferred that there is a relation between the entrepreneurship s success and the inherent competences of the entrepreneurs, such as independence and self-assurance, persistence, pride and self-esteem, creativity, innovation, intuition, taking moderate risks, perfectionism, and calling. The recognition of market opportunities is the key to the development of the activities where the derivation of occupation is the starting point. The major emphasis of its project is related to the obsessive search for quality and sustainability, aiming to the constant satisfaction of its clients. They are innovative in the Schumpeterian style, in the way that they don t necessarily associate innovation with scientific knowledge. They prospered once they had created in the market a new business strategy, as they have a high intelligence level for success.
Nos ?ltimos anos, consolidou-se a import?ncia que micro e pequenas empresas representam no cen?rio econ?mico e social do pa?s. Ao mesmo tempo, ? incontroverso ser a competitividade fundamental para a maior inser??o das empresas brasileiras em mercados cada vez mais globalizados. Nesse contexto, cresce o interesse pelo empreendedorismo. Compet?ncias empreendedoras assumem papel de destaque. O objetivo deste estudo foi identificar como tais compet?ncias empreendedoras fazem parte da hist?ria de vida dos agentes das empresas ganhadoras do Pr?mio TOP Empresarial nos anos de 2006 e 2007 e que os teria tornado bem sucedidos. Esta pesquisa adota como base a taxionomia apresentada por Vergara (2006): quanto aos fins, explorat?ria e descritiva e, quanto aos meios, bibliogr?fica, documental e de estudo de casos m?ltiplos. Os dados foram colhidos atrav?s de um question?rio semi-estruturado, enviado por e-mail aos agraciados, ap?s serem contatados pessoalmente. O tratamento e a an?lise de dados se desenvolveram atrav?s do M?todo de An?lise de Conte?do, an?lise categorial mista, onde as categorias foram definidas, a priori, com base na teoria de Man e Lau (2000). A amostra do primeiro estudo, referente aos ganhadores do Pr?mio TOP Empresarial 2006, se constitui de cinco dentre os seis empreendedores. J? a amostra do segundo estudo, ganhadores do Pr?mio TOP Empresarial 2007, se constituiu de seis dentre os oito empreendedores. Destacaram-se sete ?reas de compet?ncias empreendedoras no estudo. Os resultados mostram que tr?s destas s?o consideradas relevantes e comuns: Compet?ncia Conceitual, Compet?ncia Estrat?gica e Compet?ncia de Suporte e representam 58,77% de freq??ncia relativa, com destaque para a primeira, que representa 24,85% deste total. Depreende-se que muito do sucesso do empreendimento est? relacionado ?s compet?ncias inerentes a esses empreendedores, como: independente e autoconfian?a; persistente, orgulho e auto-estima, criatividade, inova??o, intui??o, assun??o de riscos moderados, perfeccionismo e voca??o. O reconhecimento de oportunidades de mercados se mostra fundamental, para o desenvolvimento de suas atividades, onde a deriva??o de ocupa??o ? a base. O grande destaque do seu produto est? relacionado ? busca obsessiva pela qualidade e sustentabilidade, visando ? constante satisfa??o dos seus clientes. S?o inovadores no estilo schumpeteriano, dado que, n?o associam necessariamente a inova??o ao conhecimento cient?fico. Prosperam ao criarem no mercado uma nova estrat?gia de neg?cios, pois t?m um alto n?vel de intelig?ncia para o sucesso.
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Jouida, Nejmeddine. "Modulateur sigma-delta complexe passe-bande à temps continu pour la réception multistandard." Thesis, Bordeaux 1, 2010. http://www.theses.fr/2010BOR13999/document.
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Le travail de recherche que nous présentons dans cette thèse s’inscrit dans le domaine de la conception des circuits et systèmes pour la numérisation des signaux radio large bande multistandard. La finalité de ces travaux est l’établissement de nouvelles méthodologies de conception des circuits analogiques et mixtes VLSI, et à faible consommation pour le convertisseur analogique numérique (CAN). Nous proposons l’utilisation d’un CAN de type S? complexe passe-bande à temps-continu pour l’architecture Low-IF. Ce qui permet de simplifier l’étage analogique en bande de base en esquivant le besoin de circuits tels que le contrôleur de gain automatique, le filtre anti-repliement et les filtres de rejection d’images. Le récepteur est plus linéaire et présente un degré d’intégrabilité adéquat pour les applications multistandard de type Radio logicielle Restreinte (SDR). La première contribution consiste à proposer une méthodologie originale et complètement automatisée de dimensionnement du modulateur ?? pour la réception SDR. Une nouvelle stratégie de stabilisation, basée sur le placement des zéros et des pôles du filtre de boucle, est élaborée permettant ainsi de simplifier le passage du temps-discret vers le temps-continu par une simple correspondance entre les domaines pour les intégrateurs et les résonateurs du filtre de boucle. La deuxième contribution concerne la construction d’une architecture générique du modulateur ?? complexe à temps-continu en suivant une méthodologie originale. Les éléments de base de cette architecture sont les deux modulateurs ?? passe-bas pour les voies I et Q à temps-continu. Les deux filtres de boucles sont en couplage croisé en structure polyphase, ce qui permet le décalage vers la fréquence intermédiaire du récepteur. Nous avons conçu un outil de dimensionnement sous MATLAB pour les modulateurs S? multistandard stables d’ordre élevés à temps-continu, passe bas, passe-bande réel et complexe. La troisième contribution de ces travaux concerne la proposition d’une méthodologie de conception avancée de circuits mixtes VLSI pour les CANs de type ??. Cette méthodologie de conception permet une combinaison des approches descendante ‘Top-down’ et montante ‘Bottom-up’, ce qui rend possible l’analyse des compromis de conception par l’utilisation concurrente des circuits au niveau transistor et des modèles comportementaux. Cette approche permet de faire allier à la fois la précision et la vitesse de processus de simulation lors de la conception des CANs de type ??. La modélisation comportementale du modulateur S?, en utilisant le langage VHDL-AMS, nous a permis de développé une bibliothèque de modèles permettant la prise en compte des imperfections tels que le bruit, le jitter, le retard de boucle au niveau comportemental. Afin d’illustrer la méthodologie de conception proposée, un exemple de la vérification par la simulation mixte est fourni à travers la conception d’un quantificateur en technologie CMOS. L’extraction des paramètres des imperfections du schéma au niveau transistor a permit d’enrichir le modèle comportemental et de prévenir les anomalies causant la dégradation des performances du modulateur S?Abstract
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GON?ALVES, Fernando Soares. "Efici?ncia da recupera??o ambiental de ?reas antropizadas pela minera??o de ferro do Complexo Caraj?s - PA." Universidade Federal Rural do Rio de Janeiro, 2012. https://tede.ufrrj.br/jspui/handle/jspui/1522.
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CAPES
Mining activities have a direct influence on economic development, which directly affects the gross domestic product (GDP), mainly in developing countries. The environmental interference provided by the mining activities can vary in intensity according to the mineral exploited, but they are always punctual activities. This study was conducted in the mineral province of Caraj?s, PA, in areas revegetated post mining iron ore, and to evaluate the processes of vegetation arising from revegetation of barren slopes, starting from the initial hypothesis that signed the revegetation of slopes of waste in the mining of iron using cocktails of seed species in the rainforest and savanna Metal?fila can act as facilitators of natural succession and conditioners as the substrate. The second hypothesis is that signed the removal, storage and return of surface soil organic horizon ("topsoil") after the operation by open pit mines have an essential role in the revegetation process, because they already provide quantitative and qualitative characteristics that reflect the communities previously existing in the area. In this work were highlighted four areas of study, three areas undergoing revegetation and an area with the presence of primary forest. The variables analyzed were phytosociology, photosynthetically active radiation, chemical quality of the stock of litter, soil fauna and soil fertility. The results indicate that the revegetation processes used, promoted the formation of a plant community which facilitates the process of succession over time, as many of the species used in the cocktail of seeds of the CCI and workshop areas are being replaced by species more adapted to conditions of each site. In the area called Hoppe, the herbaceous species that colonized the area are being replaced by shrub and tree species. The quality of the provided information able to litter cluster areas of studies according to the nutrients present in the litter, the areas being divided into Workshop CCI and the concentrations of lignin, cellulose, P and Mg, while areas were grouped Hoppe and Mata as a function of P and N fractions present in the twigs and leaf litter. But the use of soil fauna indicated that the groups Collembola and Formicidae will be extremely important for monitoring the quality of sites over time. From these results it was concluded that the use of biotic and abiotic indicators in studies of recovery of mined areas should be thoroughly studied and documented, they offer attributes able to understand developments in the recovery process.
As atividades de minera??o t?m influencia direta sobre o desenvolvimento econ?mico, afetando diretamente o produto interno bruto (PIB), principalmente dos pa?ses em desenvolvimento. As interfer?ncias ambientais proporcionadas pelas atividades de minera??o podem variar de intensidade de acordo com o mineral explorado, mas sempre s?o atividades pontuais. O presente estudo foi realizado na prov?ncia mineral de Caraj?s-PA, em ?reas revegetadas p?s minera??o de min?rio de ferro, tendo como objetivo avaliar a forma??o vegetal oriunda dos processos de revegeta??o de taludes de est?ril, partindo da primeira hip?tese firmada que a revegeta??o dos taludes de est?ril em ?rea de minera??o de ferro utilizando coquet?is de sementes de esp?cies pertencentes a savana metal?fila e floresta ombr?fila podem agir como facilitadores da sucess?o natural e como condicionadores do substrato. A segunda hip?tese firmada ? que a retirada, armazenamento e retorno do horizonte org?nico superficial do solo (?topsoil?) ap?s a explora??o por minera??es a c?u aberto t?m fundamental papel no processo de revegeta??o, por j? apresentar caracter?sticas quantitativas e qualitativas que refletem as comunidades anteriormente existentes na ?rea. Neste trabalho foram demarcadas quatro ?reas de estudo, sendo tr?s ?reas em processo de revegeta??o e uma ?rea com presen?a de mata prim?ria. As vari?veis analisadas foram fitossociologia, radia??o fotossinteticamente ativa, qualidade qu?mica do estoque de serrapilheira, fauna do solo e fertilidade do solo. Os resultados obtidos apontam que os processos de revegeta??o utilizados, promoveram a forma??o de uma comunidade vegetal que viabilize o processo sucessional ao longo do tempo, pois muitas das esp?cies utilizadas no coquetel de sementes das ?reas CCI e Oficina est?o sendo substitu?das por esp?cies mais adaptadas ?s condi??es de cada s?tio. Na ?rea denominada Hoppe, as esp?cies herb?ceas que colonizaram a ?rea est?o sendo substitu?das por esp?cies arbustivas e arb?reas. A qualidade da serrapilheira forneceu subs?dios capazes de agrupar as ?reas de estudos em fun??o dos nutrientes presentes na serrapilheira, sendo as ?reas CCI e Oficina agrupadas em fun??o das concentra??es de lignina, celulose, P e Mg, enquanto que as ?reas Hoppe e Mata ficaram agrupadas em fun??o do Ca e N presente nas fra??es galhos e folhas da serrapilheira. J? a utiliza??o da fauna do solo indicou que os grupos Collembola e Formicidae ser?o de extrema import?ncia para o acompanhamento da qualidade dos s?tios ao longo do tempo. A partir destes resultados foi poss?vel concluir que a utiliza??o de indicadores bi?ticos e abi?ticos em estudos de recupera??o de ?reas mineradas devem ser amplamente estudados e documentados, pois oferecem atributos capazes de compreender a evolu??o nos processos de recupera??o.
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Conte, Talita Cristiane. "Influência das HSPs (heat shock proteins) e do mTORC-1 (mammalian target of rapamycin complex 1) na regeneração de músculos esqueléticos." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/42/42131/tde-26012010-111321/.
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O objetivo deste trabalho foi contribuir para o melhor entendimento dos mecanismos intracelulares envolvidos na regeneração muscular esquelética, através do estudo da influência das proteínas de choque térmico (HSPs) e do mTORC1 (mammalian target of rapamycin complex 1) no processo regenerativo muscular. O tratamento com radicicol (indutor de HSPs) em músculos lesados induziu aumento da área de secção transversal das fibras musculares em 10 e 21 dias após lesão e aumento do número de células satélites e de fibras musculares em diferenciação em 1 e 10 dias após lesão, respectivamente, quando comparado aos seus respectivos controles apenas lesados. O tratamento com rapamicina (inibidor de mTORC1) em músculos lesados induziu uma diminuição maior da área de secção transversal das fibras musculares em 10 e 21 dias após lesão e menor síntese protéica muscular em 10 dias após lesão quando comparado aos músculos somente lesados. Nossos resultados sugerem que as HSPs e o mTORC1 são importantes para o processo de regeneração muscular esquelética.The goal of this work was to contribute to a better understanding about the intracellular mechanisms involved in skeletal muscle regeneration by studying the influence of heat shock proteins (HSPs) and mTORC1 (mammalian target of rapamycin complex 1) in the muscle regeneration process. The treatment with radicicol (a HSP inductor) in injured muscles induced increase of myofiber cross section area at 10 and 21 days post lesion and increased number of satellite cells and differentiating myofibers at 1 and 10 days post lesion, respectively, when compared to their respective injured controls. The treatment with rapamycin (a mTORC1 inhibitor) in injured muscles induced a more accentuated decrease in myofiber cross section area at 10 and 21 days post lesion and decreased muscle protein synthesis at 10 days post lesion when compared to only-injured muscles. Our results suggest that HSPs and mTORC1 are important to the process of skeletal muscle regeneration.
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Gaio, Michele. "Light emission in complex networks from single-photons to lasing." Thesis, King's College London (University of London), 2016. https://kclpure.kcl.ac.uk/portal/en/theses/light-emission-in-complex-networks-from-singlephotons-to-lasing(137751c8-fa87-48da-90d0-c632168636e9).html.
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Light-matter interaction, which is at the heart of the science and technology of light, can be controlled and designed in materials structured at the nanometre length-scale, for enhanced light emission and absorption, and down to the single photon level. The aim of this thesis is to investigate complex photonic geometries, i.e. systems where the collective interaction of a large number of constituents denes the optical properties with emergent phenomena beyond the sum of the response of the individual constituents. In particular, a central topic is the emission of light from sources located in dielectric and plasmonic networks with dierent degree of disorder and correlation. Experimental and theoretical evidence of coupling of single photons to propagating modes in nano-waveguides, emission enhancement in plasmonic structures, and collective emission in disordered lasing systems are presented. Large coupling of individual quantum dots embedded in free-standing sub-wavelength waveguides is experimentally demonstrated. These waveguides are fabricated by electrospinning, a scalable technique suitable for the realisation of large interconnected systems. Light emission enhancement is investigated in plasmonic self-assembled systems and lithographic structures, which build on the framework of optical antennas and allows isolating local and global contributions to the local density of states around a topological percolation phase transition. One of the most important cooperative eects between multiple emitters is lasing. Random lasing is investigated numerically and experimentally in diusive systems with particular attention to the spectral properties of the emission and its relation with the physical and chemical parameters of the surrounding environment, which can be exploit to tune the lasing emission, thus providing a novel sensing scheme. These results provide the building blocks to construct a photonic network of emitters coupled by simple optical links.
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Cheikh, Aimane. "Etudes des hétérostructures à bases d'oxydes complexes pour de potentielles utilisations en cellules solaires." Thesis, Normandie, 2020. http://www.theses.fr/2020NORMC208.
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Grace à leurs propriétés fonctionnelles prometteuses, l’étude des oxydes ternaires à base de vanadium déposés sous forme de couche mince ont suscité beaucoup d’intérêt et ont fait l’objet d’une activité intense en recherche dans le domaine optoélectronique et photovoltaïque.Durant ce travail de thèse, on a étudié dans un premier temps la possibilité d’utiliser les métaux fortement corrélés tel que SrVO3 comme étant un oxyde transparent et conducteur (TCO). Pour cela, on a étudié l’évolution des propriétés optoélectroniques en fonction des conditions de croissance du SrVO3 déposé sous forme de couche mince. Dans un deuxième temps, notre étude s’est focalisée sur la réalisation d’une ingénierie de cellule solaire basée sur les hétérostructures tout oxyde de différentes bandes interdites. Pour cela, par un choix judicieux de la largeur de bande interdite de certaines pérovskites, nous avons synthétisé le LaVO3, dont l’absorption est optimale dans le spectre solaire, sur un substrat SrTiO3 sous différentes conditions de croissance. Du point de vue optique, l’étude des hétérostructures LaVO3/SrTiO3 déposé à basse pression d’oxygène a mis en évidence que le film LaVO3 possède une bande interdite de 1.18 eV se situant dans la plage optimale pour le photovoltaïque. Du point de vue électrique, l’interface polaire LaVO3/ SrTiO3 génère une couche d’interface conductrice qui servira de contact électrique pour les cellules solaires. Un autre intérêt du LaVO3 est sa structure cristalline commune à un grand nombre d’oxydes possédant des différentes valeurs des bandes interdites. Pour réaliser notre système, nous avons choisi en particulier la pérovskite LaFeO3 ayant une bande interdite de 2.2 eV, supérieure à celle du LaVO3 afin d’améliorer l’absorption optique à haute énergie. Une fois les propriétés optoélectroniques ont été établies, nous avons synthétisé l’empilement LFO/LVO sur un substrat SrTiO3 à basse pression d’oxygène. L’évolution des propriétés de notre système en fonction de l’épaisseur de LaFeO3 déposé est également étudié, mais jusqu’à présent aucune propriété de photoconductivité n’a été obtenueDue to their promising functional properties, ternary oxide thin films based on Vanadium have gained much research interest in photovoltaic technologies.During this work, we first studied the possibility to use the strongly correlated metal SrVO3 as a transparent conducting oxide (TCO). For this reason, we have studied the optoelectronic properties of SrVO3 under different growth conditions. Second, our study was focused on making band gap-graded design solar cells based on oxide heterostructures. LaVO3 is particularly interesting due to its optical band gap localized in the optimal range for harvesting solar light. Accordingly, the LaVO3 was synthetized on SrTiO3 substrate under different growth conditions. Optical measurements reveal that LaVO3/SrTiO3 heterostructure grown at low oxygen pressure possess a band gap of 1.18 eV in the ideal energy range for photovoltaic. Electrical properties show that the interface LaVO3/ SrTiO3 is conducting, serving as an electrical contact for solar cells. Another interest of LaVO3 is its crystalline structure offering the possibility to combine it with other structurally compatible transition metal oxides with larger band gap such as LaFeO3 (2.2 eV) in order to enhance the optical absorption at high energy. Once the optoelectronic properties have been established, the LFO/LVO heterostructure was synthetized on SrTiO3 substrate at low oxygen pressure. The physical properties of our system have been also investigated for different LaFeO3 thickness but, to date, no photoconductivity was obtained
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Teleha, Mary Ann. "Evidence for a Direct Link between the Tol-Pal Protein Complex and Gram Negative Bacteria Cell Division via an Interaction between TolQ and the Divisome Protein FtsN." Bowling Green State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1370913242.
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