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Dissertations / Theses on the topic 'Tomatoes Plant tissue culture'

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Published: 4 June 2021

Last updated: 14 February 2022

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1

Compton, Michael E. "De novo morphogenesis on tomato thin cell layers and variation for genetic recombination among plantlets regenerated from tissue culture." Diss., This resource online, 1990. http://scholar.lib.vt.edu/theses/available/etd-09162005-115005/.

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2

Carvalho, Rogério Falleiros. "Uso de mutantes fotomorfogenéticos no estudo da competência para regeneração in vitro em micro-tomateiro (Lycopersicon esculentum CV Micro-Tom." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-19022004-103113/.

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Paralelamente ao modelo Arabidopsis thaliana, o tomateiro (Lycopersicon esculentum) tem sido crescentemente utilizados em abordagens genéticas de questões fisiológicas. Uma das principais vantagens de Arabidopsis como planta de laboratório tem sido seu pequeno porte e ciclo de vida curto. Contudo, a cultivar Micro-Tom (MT) de tomateiro possui tamanho muito reduzido (8 cm) e pode produzir até 5 gerações por ano. Mutantes fotomorfogenéticos em tomateiro deficientes na síntese do cromóforo do fitocromo (au), mutantes deficientes na síntese das apoproteínas PHYA e PHYB1 (fri e tri, respectivamente) e mutantes superexpressando o fitocromo (hp, atv e Ip) constituem-se em um modelo para estudos da fotomorfogênese. No que se refere à capacidade de regeneração in vitro como uma resposta fotomorfogenética, poucos trabalhos têm sido realizados. O presente trabalho teve como objetivo transferir as mutações au, fri, tri, hp, Ip e atv, bem como o locus de regeneração (Rg1) da cultivar MsK, para a cultivar Micro-Tom. As linhagens obtidas foram utilizadas para verificar o efeito da fotomorfogênese na competência para regeneração in vitro. Para tanto, foram realizados tratamentos com luz branca, vermelho (V) e vermelho-extremo (VE) em explantes radiculares, caulinares e foliares do genótipo micro-MsK em meio MS mais 5mM de BAP e tratamentos com luz branca em explantes radiculares, caulinares e foliares de micro-mutantes fotomorfogenéticos também em meio MS mais 5mM de BAP. Para todos os tratamentos utilizou-se a cultivar MT como controle. Sob V, as raízes de micro-MsK apresentaram-se diferenciadas, enquanto sob VE não ocorreu diferenciação. O maior número de gemas formadas tanto para caule quanto para folhas de micro-MsK ocorreu sob V, enquanto sob VE foi observado um decréscimo na formação de gemas. A partir destes resultados sugere-se que a forma ativa do fitocromo, induzida pelo V, interage com o Rg1 na aquisição de competência para regeneração. Nos tratamentos com luz branca, raízes de micro-MsK e de mutantes micro-hp, micro-atv e micro-Ip apresentaram-se diferenciadas, enquanto não houve diferenciação para o mutante micro-au ou para o controle MT. O número de gemas formadas alcançou maiores valores para folhas de micro-hp e micro-Ip e a para caules de micro-atv. Apenas um número muito reduzido de gemas foi formado a partir de folhas de micro-au. Com base na alta competência para regeneração de micro-MsK e de mutantes que superexpressam o fitocromo, sugere-se que o fitocromo promove, em uma via de sinalização, a indução de fatores de regeneração (Rg1). Alternativamente, o locus Rg1 poderia promover a alta capacidade regenerativa tornando os explantes mais competentes ao efeito da superexpressão do fitocromo, o qual poderia induzir outros fatores de regeneração.<br>Parallel to Arabidopsis thaliana model, the tomato (Lycopersicon esculentum) has been increasingly used as a genetic approach to address physiological questions. One of the main advantages of Arabidopsis as a laboratory plant has been its small size and short life cycle. However, the tomato cultivar Micro-Tom (MT) possesses reduced size (8 cm) and can produce up to 5 generations per year. Tomato photomorphogenic mutants deficient for the synthesis of phytochrome chromophore (au) or the apoprotein PHYA and PHYB1 (fri and tri, respectively), as well as mutants superexpressing phytochrome (hp, atv and Ip) consist on a model to study photomorphogenesis. Concerning the in vitro regeneration capacity as a photomorphogenic response, fewer works have been carried through. The current work aimed at transfering the mutations au, fri, tri, hp, Ip and atv, as well as the regeneration locus (Rg1) of cv MsK to the cv Micro-Tom (MT). The genotypes obtained were used to verify the effect of photomorphogenesis on the competence for in vitro regeneration. Root, stem and leaf explants from MT and Micro-MsK were incubated in MS plus 5mM BAP under white, red (R) and far-red (FR) light. Root, stem and leaf explants from MT and photomorphogenic micro-mutants were incubated in MS plus 5mM BAP under white light. Under R, roots of micro-MsK were presented differentiation, while under FR the differentiation did not occur. Under R, stem explants from micro-MsK formed more shoots than did leaf explants, while under FR was observed a decrease in shoot formation for all types of explants. These results suggest that the active form of phytochrome, induced by R, interacts with the Rg1 in the acquisition of competence for regeneration. In the treatments with white light, roots of micro-MsK and of mutants micro-hp, micro-atv and micro-Ip presented differentiation, while no differentiation was observed for the mutant micron-au or control MT. The number of shoots formed reached the highest values for leaf explants of micro-hp and micro-Ip and for stem explants of micron -atv. Only a low number of shoots was formed from micro-au leaf explants. On the basis of the high competence for regeneration of micro-MsK and mutants that super express phytochrome, it is suggested that the phytochrome promotes, in a signaling pathway, the induction of regeneration factors ( Rg1 ). Alternatively, the Rg1 locus may turn the explant most competent to respond to phytochrome, which could induces others regeneration factors.

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3

Slomka, Marek Jozef. "Studies of tomato golden mosaic virus in plants, protoplasts and tissue culture." Thesis, Imperial College London, 1987. http://hdl.handle.net/10044/1/46616.

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4

Sheibani, Ahmad. "Tissue culture studies of Pistacia." Thesis, University of Salford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.238801.

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5

Al-Ani, Nabeel K. "Some epigenetic effects in plant tissue culture." Thesis, Aberystwyth University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.659362.

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6

VanTine, Melissa C. "Effect of watering regime and media components on the production of organic tomato transplants." Morgantown, W. Va. : [West Virginia University Libraries], 2004. https://etd.wvu.edu/etd/controller.jsp?moduleName=documentdata&jsp%5FetdId=3619.

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Thesis (M.S.)--West Virginia University, 2004.<br>Title from document title page. Document formatted into pages; contains vii, 60 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 56-60).

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7

James, V. J. "Regulation of xenobiotic catabolism in plant tissue culture." Thesis, Cardiff University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380205.

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8

Wardrop, Julie. "Biotechnological applications of perfluorochemical liquids in plant tissue culture." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389475.

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9

Al, Kaabi Helel Humaid Saed Humaid. "Date palm tissue culture and AFLP analysis of plant variability." Thesis, Imperial College London, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409314.

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10

Ghadimzadeh, Mortaza. "Studies of protoplast and liposome techniques in plant tissue culture." Thesis, University of Salford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.255239.

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11

Khalid, Norzulaani. "Somaclonal variation through tissue culture studies in Chrysanthemum morifolium." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329847.

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12

Danon, Avihai. "Molecular events associated with halophytic growth in Lycopersicon pennellii." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184642.

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We have studied the effects of exogenous salt on whole plant and suspension culture cells of the halophytic tomato Lycopersicon pennellii. Under low salt conditions (2.9 dS/M) plants showed enhanced (halophytic) growth (107% of control). At moderate (7.5 dS/M) and high (18.5 dS/M) salt levels, salt stress reduced growth to about 78% and 40% of control respectively. Salt-induced changes in root mRNAs were analyzed via two-dimensional PAGE of cell free translation (CFT) products. We have identified 14 proteins whose levels were enhanced by exogenous salt. One of these proteins was unique to low salt induced halophytic growth. This system allowed for discrimination between proteins up-regulated at all salt levels and those up-regulated only during salt stress induced growth reduction. Ten proteins were identified whose levels were reduced by exogenous salt. Once again, one could identify a subset of proteins whose levels were reduced only under salt stressed conditions. Proteins identified in this study are candidates for roles in growth maintaining stress adaptive metabolism in L.pennellii. These data underscore the complexity of the genetic control of salt metabolism in higher plants. The effects of exogenous salt on protein synthesis and accumulation were studied in suspension cultures of L.pennellii. Two salt levels were applied to the cells. Under low salt conditions (LS, 10 mM), L.pennellii cells showed enhanced (halophytic) growth. Under high salt conditions (HS, 50 mM), the cells showed reduced (salt-stressed) growth. Changes in proteins with time were analyzed by a combination of cell free translation, in vivo labeling and total accumulated protein. In vivo labeling studies showed that the pattern of steady state protein synthesis was disrupted shortly after addition of salt. High salt induced greater disruption in the pattern. Over time, the steady state levels of most proteins shifted back towards those of the unstressed-control. However, the level of several proteins remained altered. Analysis of proteins whose levels increased with exogenous salt showed differences in the response patterns that may allow for discrimination between proteins involved in growth maintaining and stress shock responses.

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13

Taeb, Abdulkarim Giumaa. "Influence of culture environment on tulip micropropagation." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328788.

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14

Watson, L. D. "Induction and assessment of plant cell membrane permeability." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233331.

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This thesis describes the isolation, immobilisation and permeabilisation of <i>Digitalis lanata</i> (foxglove) and <i>Nicotiana tabacum</i> (tobacco) plant cells and protoplasts. Protoplasts were isolated from leaves of <i>Digitalis lanata</i> and <i>Nicotiana tabacum</i> and cultured in conditions of varying osmotic potential, illumination and cell density in order to achieve maximum cell stability. The regeneration of cellulose cell walls in <i>Nicotiana tabacum</i> protoplasts could be inhibited by the addition of dilute concentrations of the herbicide 2,6-Dichlorobenzonitrile. At concentrations of 2-10 mg/l in culture medium cell wall regeneration could be prevented for up to 6-8 weeks. Isolated cells and protoplasts were stabilised by entrapment within a beaded agarose support matrix. <i>Nicotiana tabacum</i> protoplasts and cells, immobilised within an agarose beaded support matrix were used to develop a technique to permeabilise and destabilise the cell membranes. Cells or protoplasts pre-loaded with 6-Carboxy-Fluorescein, Neutral Red stains and <SUP>14</SUP>C-Sucrose or <SUP>86</SUP>Rb<SUP></SUP>+ radioactive tracers were employed as markers for cell permeability or leakiness. Efflux or Compartmental analysis was used to determine the influence of various selected permeabilising agents on the integrity and the leakiness of either protoplast or cell membranes. Immobilised protoplasts or cells were subjected to a three - step procedure involving initial loading with <SUP>86</SUP>Rb<SUP></SUP>+ tracer, a membrane permeabilisation step and finally a recovery step. Protoplast membrane stability could not be regained after the recovery step, following a 30 minute period of permeabilisation with 50 mM acetic acid in culture medium. Immobilised cells could, however, regain membrane integrity with good intracellular retention of <SUP>86</SUP>Rb<SUP></SUP>+ tracer ion during the efflux experiment. Thus, immobilised <i>Nicotiana tabacum</i> cells could be made reversibly permeable with the use of specific agents. It is anticipated that such techniques which encourage reversible permeabilisation of immobilised cells have potential in larger scale plant cell culture systems to effect the release of intracellularly stored, useful secondary metabolites. There are also possibilities for the inclusion of exogenous precursors, cofactors and foreign genetic material which would increase compound yield and may produce novel secondary metabolites.

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15

Lappin, G. J. "The biotransformation of monoterpenoids by plant cells in axenic culture." Thesis, University of Westminster, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382797.

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16

Blakemore, Philip Alexander. "Optimisation of steam reconditioning for regrowth-ash and plantation-grown eucalypt species." Connect to full text, 2008. http://ses.library.usyd.edu.au/handle/2123/2343.

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Thesis (Ph. D.)--University of Sydney, 2008.<br>Includes graphs and tables. Includes list of publications: p. iv. Title from title screen (viewed May 5, 2008). Thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the School of Chemical and Biomolecular Engineering. Includes bibliographical references. Also available in print form.

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17

Heyenga, Gerard. "Tissue culture of Podophyllum hexandrum and production of anticancer ligands." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235985.

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18

Gribble, Karleen Dawn. "Towards an understanding of the physiological abnormality of tissue cultured plants known as vitrification /." [Richmond, N.S.W.] : Horticulture, University of Western Sydney, Hawkesbury, 1999. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030513.144109/index.html.

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Thesis (Ph.D.) -- University of Western Sydney, Hawkesbury, 1999.<br>Thesis submitted for the degree of Doctor of Philosophy. Spine title: Towards an understanding of vitrification in tissue cultured plants. Includes bibliographical references (leaves 175-203).

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19

Redway, F. A. "Regeneration in tissue and protoplast culture of Sainpaulia ionantha (African violet)." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382673.

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20

THOMAS, JOHN CALVIN. "THE CONSEQUENCES OF BROMODEOXYURIDINE TREATMENT IN PLANT TISSUE CULTURES (REGENERATION, REPLICATION)." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183989.

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Plant tissue culture regeneration is chiefly regulated by exogenous phytohormones. To stop regeneration and induce undifferentiated callus growth auxins are used. Unfortunately auxins influence many plant responses, most unrelated to development. Using the thymidine analogue 5-bromodeoxyuridine (BrdU) a phytohormone independent means for differentiation inhibition has been developed. Studies were focused on the target site and mechanism of BrdU action. The BrdU inhibited step in development is indicative of a plant response necessary for normal differentiation. BrdU (5-30 uM) interrupts callus growth in all tested plants. Exogenous cytokinin does not restore growth while thymidine and deoxycytidine rescue plant growth and differentiation in the presence of BrdU. Endogenous cytokinin levels are not greatly affected by subtoxic BrdU levels and indicate that cytokinin and BrdU act upon independent sites. Domestic carrot cells were used in further studies. Normally carrot cells are undifferentiated in medium with the auxin 2,4D. When 2,4D is removed, somatic embryogenesis takes place. By including 5 uM BrdU in the hormoneless medium, the cells fail to differentiate. The growth of carrots in 2,4D is not affected by 5 uM BrdU. Thus, BrdU influences growth during differentiation to a greater extent than the growth of callus cells. BrdU is effective in halting development when applied 0-24 hours after differentiation induction. An event required for differentiation (the first and second replications) must take place at this time. BrdU action begins with DNA incorporation. The consequent cellular replication becomes slowed and DNA repair results. At the same time RNA and protein levels are similar in BrdU treated and untreated cultures. BrdU thymidine substitution into DNA increases from 28% (2 days) to 68% (3 days) after embryogenic induction. A second BrdU effect follows DNA incorporation. Factors (MIFs) in the medium of BrdU treated cells arrest differentiation. After BrdU is repaired from the DNA, the cells are only able to differentiate after a medium change. Understanding MIF production could explain why some plants differentiate more readily than others. BrdU provides the means for further study of MIF's in the auxin-free inhibition of development.

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21

Qouta, Lolita Abdulla. "The biochemistry and molecular biology of intercellular adhesion in plant tissue culture." Thesis, University of Glasgow, 2008. http://theses.gla.ac.uk/302/.

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The adhesion between neighbouring plant cells is established as cells are formed during cytokinesis through the middle lamella that is made principally of pectins and proteins. Pectins are secreted into the cell wall in a highly methylesterified form and subsequently de-esterified in muro by pectin methyl esterase (PME, E.C. 3.1.11). The present study reports on the biochemical characterization and immunochemical analyses of phosphate buffer/EDTA pectic extracts associated with cell-cell adhesion in suspension cultures of wild type (WT), salt tolerant (HHS) cell lines and synchronized Arabidopsis suspension cultures. Using the synchronized cultures, The PME-mediated configuration of pectins at the onset of adhesion during cytokinesis, was assessed through the analysis of the expression patterns of the PME isoforms annotated to be expressed throughout the cell cycle The wild type Arabidopsis seemed to maintain the intercellular adhesion through the gelling of the highly methylated JIM7 recognized homogalacturonans that were shown to be abundant in the primary cell walls, middle lamellae and cellular junctions, possibly due to the hydrophobic interactions between the methoxy groups. The rhamnogalacturonan-I fraction was rich in arabinan side chains reflecting the proliferative state of the cells. The increase in arabinan content was accompanied by a reduction in the galactan content 4 days after subculturing. The cell walls of salt tolerant Arabidopsis contained the JIM7 and LM7recognized epitopes along with a high degree of branching of rhamnogalacturonan-I carrying galactans and arabinans as side chains. The change in the detected epitopes is thought to play a role in the ability of the cells to withstand the high osmotic pressure and increase the in the level of adhesion between cells. The JIM5 low methylesterified HGs were less abundant in both cultures, and the absence of the 2F4 antibody recognizing the Ca2+ egg boxes could be attributed to the scarce amounts of Ca2+ present in the culturing medium The immunochemical studies of the pectin extracted from the synchronized Arabidopsis suspension cultures after washing out aphidicolin indicated that the recognition of both of JIM7 and JIM5 varied in parallel during the cell cycle, whereas, the recognition of arabinan increased during the cell division. The sequence and phylogenetic analysis of ten PME isoforms that were annotated to be expressed at one or more phases of the cell cycle of synchronized Arabidopsis thaliana suspension cultures (Menges and Murray, 2002 and 2003), revealed that only five of these genes could be PMEs. The genes At4g02330, At1g02810, At2g26440, and At2g47550 were thought to be of type II PMEs which have a pre-pro-catalytic domains and At5g47500 is a type I PME that lack the pro-region. The amino acid sequence of At4g12390 showed similarities with the N-terminal pro-peptides of plant PME and invertase inhibitors. The expression of several PME genes was studied in suspension cultures of Arabidopsis thaliana synchronised using aphidicolin. Semi-quantitative PCR experiments showed that the expression of At5g47500 transcript was always detected during M phase of the cell cycle. The rest of the genes failed to show consistent patterns of expression. Northern blots revealed that mRNA coding for At5g47500 decreases during S and G2 phases and accumulates during the M phase of the cell cycle. Our results suggest that this PME isoform is involved in the modulations of the cell walls as the cells are going through division and cytokinesis.

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22

Hussain, Shayne. "Evaluation of microbial extracts for contamination control in plant tissue culture systems." Thesis, University of Plymouth, 1991. http://hdl.handle.net/10026.1/2716.

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Culture filtrates of 13 microbial antagonists exhibited in vitro growth inhibition of a range of test contaminations of herbaceous and woody plant tissue culture systems. Filtrates produced by Bacillus subtilis and Trichoderma viride isolates displayed the greatest broad- range inhibitory activity. Microscopic analysis of antagonized fungal mycelia revealed altered hyphal morphology. Maximum filtrate inhibitory activity was produced when selected antagonists were cultured within a pH range of 5-7 and a temperature range of 20-35°C. Filtrates were thermo-stable at 70°C and could be stored for up to 4 weeks with only a minimal reduction in their inhibitory activity. Bulk-volume production of inhibitory filtrates of a T. viride isolate was achieved by optimization of fermentation pH, temperature, and aeration conditions. Plant culture species displayed different responses when grown on media incorporated with microbial filtrates. Microscopy studies at the cellular level revealed reduced cell densities and cellular distortion in plant tissues treated with phytotoxic doses of microbial filtrates. Non-phytotoxic doses of filtrated produced by the B. subtilis and T. viride isolates produced a reduction in the density of opportunistic contaminations in herbaceous plant tissue cultures when applied as prophylactic treatments. Microbial filtrates proved totally ineffective when employed as post-infection sterilants in contaminated plant cultures. The efficacy of selected microbial filtrates was not comparable to that of conventional antibiotics when assessed for their ability to control contamination levels in herbaceous and woody plant culture systems. Further purification of microbial filtrates for enhanced inhibitory activity is discussed along with the possibility of co-cultivation of micro-organisms with plant tissue cultures as a means of biocontrol of phytopathogens.

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23

Ubaid, R. H. "Plant tissue culture and prostaglandin production from a range of Allium species." Thesis, University of Salford, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381734.

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24

Mao, Ashiho A. "Tissue culture of a range of plant species from north-east India." Thesis, University of Reading, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308055.

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25

Sinha, Debleena. "Development of an In Vitro Protoplast Culture System for Albizia Lebek (L.) Benth., an Economically Important Leguminous Tree." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc500422/.

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An in vitro system of generating protoplasts from their callus cultures was established. The friable callus was more productive in terms of producing protoplasts than the green compact callus. The concentration of the various cell wall degrading enzymes had an effect on the viability of the protoplasts in the medium. The protoplast system developed from the experiments was stable and could be used for the transformation experiments of Albizia lebek and for other plant improvement practices.

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26

Patil, Rajashekar M. "Tissue culture and transformation of rice (oryza sativa L.) using tobacco nurse cells /." Title page, contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09APSM/09apsmp298.pdf.

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27

Hamidoghli, Yousef. "Production and identification of interspecific potato somatic hybrids." Thesis, University of the West of Scotland, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283091.

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28

Holden, Peter Richard. "Variability in cultured cells of Capsicum Spp." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/10960.

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29

Spencer, Andrew. "The development of shooty teratomas in Mentha species by genetic manipulation and studies on their growth and terpene production in vitro." Thesis, University of East Anglia, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292258.

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30

Gibbs, Margaret Joan. "Genetic engineering of the forage legume Lotus corniculatus using Agrobacterium : mediated transformation systems." Thesis, Durham University, 1991. http://etheses.dur.ac.uk/6040/.

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Gene transfer vectors based on the Agrobacterium tumefaciens Ti plasmid were used to develop a successful disarmed Agrobacterium tumefaciens-mediated transformation method for Lotus comiculatus. A binary vector construct, pJIT73, was used during the development of the Agrobacterium tumefaciens transformation system due to its selectable (Aph IV, nos- neo) and scorable markers. The effects of the antibiotics geneticin (G-418) and hygromycin B were studied. Use of kill curves and selection delay experiments allowed potentially suitable selection pressure parameters to be proposed. Using such selection during transformation experiments led to further optimisation of this stage of transformation. The influence of plant hormones on the regeneration of Lotus comiculatus explants was investigated and a modification of an established protocol using leaf explants was introduced as an attempt to reduce the overall time of regeneration. Various explants were used but leaf pieces were chosen as the most suitable explant on which to focus research. So, through alteration of various stages, including length of cocultivation and subsequent decontamination within the transformation process, a successful method was developed. Experiments indicated the optimum Agrobacterium tumefaciens strain to be used with Lotus comiculatus was the disarmed Ach5 type, LBA4404(pAL4404). Transgenic Lotus comiculatus plants were produced which expressed the scorable marker β-Glucuronidase gene (GUS) and the selectable marker for hygromycin B resistance, AphIV. Gene transfer was confirmed by Southern blotting. The new Agrobacterium tumefaciens-mediated vector system was used to introduce the cowpea trypsin inhibitor gene (CpTi) into Lotus comiculatus. However, although there was evidence for transformed callus development, no shoots were induced. By the use of previously established Agrobacterium rhizogenes-mediated system, an attempt was made to introduce the pea lectin gene (psl) into Lotus corniculatus. Hairy root regenerants were produced but genetic transfer was unconfirmed and attempted investigation of the plant - Rhizobium symbiosis involving Lotus corniculatus was not fulfilled.

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31

Khatun, Asma. "The genetic manipulation of jute (Corchorus) species." Thesis, University of Nottingham, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335652.

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32

Smith, Elaine Francis. "The preparation of micropropagated plantlets for transplantation." Thesis, University of East London, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.258546.

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33

Hudson, G. "Micropropagation and low temperature storage of Dieffenbachia." Thesis, University of East London, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370763.

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34

Kirby, Nigel John. "An investigation of metabolite release from plant cells in vitro to their surrounding medium." Thesis, University of the West of England, Bristol, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329861.

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35

Satchwell, Christa Elizabeth. "Investigation of the nutrient requirements of Pinus caribaea Morelet in vitro." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305742.

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Gunasekare, M. T. K. "In vitro culture directed towards plant improvement of tea (Camellia sinensis var. assamica)." Thesis, University of Southampton, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241937.

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Kaparakis, Georgios. "In vitro culture of pepper (Capsicum annuum L.)." Thesis, University of Nottingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297989.

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Wilson, Zoe Amanda. "A study of the tissue culture and genetic manipulation of rubber (Hevea brasiliensis)." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235687.

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Zalat, Eman S. "Plant tissue and cell culture of taxus species as a source of taxanes." Thesis, University of Manchester, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.488110.

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Borrino, E. M. "Plant tissue culture : an analysis of variation of in-vitro response to salinity." Thesis, University of Reading, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316040.

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Tan, Hooi Sin. "Proteomics analysis of somatic embryogenesis in tissue culture of oil palm (Elaeis guineensis Jacq)." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/33755/.

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Oil palm is an important commercial crop in Malaysia where Malaysia is the second largest producer and exporter of palm oilin the world. In order to meet the increasing demand for palm oil, elite oil palm planting materials with higher palm oil yield are the desirable planting materials. Hence, the oil palm plantation companies have incorporated in vitro micropropagation technique through somatic embryogenesis in producing elite oil palm. However, low embryogenesis rate has hampered large production of elite oil palm ramets. In this study, proteomic technology was deployed to compare protein expression and identify differential expressed protein between high and low proliferated embryogenic lines of oil palm tissue culture. From the study, total protein of oil palm young and old leaves was extracted using an optimized trichloroacetic acid/acetone precipitation protocol followed by polyethylene glycol (PEG) fractionation to isolate low abundance proteins. Then, the extracted proteins were separated on two-dimensional (2D) gel electrophoresis and protein profiles between the high and low proliferated embryogenic lines were compared. Total of 40 differentially expressed protein spots were isolated from the 2D gel for mass spectrophotometry (MS/MS) identification. However, only 26 out of 40 protein spots were identified and just 8 of the identified protein spots were isolated from young leaves. Quantitative real-time PCR were conducted on 17 proteins candidates to study on the relationship between the protein and mRNA expression level. There was 29% of the 17 proteins’ expression showed linear correlation with their mRNA expression. These proteins candidates were highlighted for further validation in the future.

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Lormand, Katherine Bradbury 1961. "RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS." Thesis, The University of Arizona, 1987. http://hdl.handle.net/10150/276474.

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The responses of the tepary bean (Phaseolus acutifolius) to in vitro tissue culture systems were documented. Tests were conducted to identify the optimal auxin and cytokinin combinations required for optimal callus growth. Regeneration experiments were conducted to: (1) determine the effect of explant source and age on regeneration, (2) effect of callus age on regeneration, (3) the cultivation status of the explant source, and (4) the effects of nutritional additives on somatic embryogenesis. The callus was easily induced and maintained in all hormonal medias except those containing IAA and 6BA. The results for regeneration were most promising from cultures derived from immature cotyledon tissue. Ammonium Chloride, glutamine and Absisic acid appeared to have little affect on embryogenesis, however the addition of kinetin enabled the embryos to develop to the torpedo stage. Callus age and cultivative status of explant source had no effects on plantlet regeneration.

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Obae, Samuel G. "Genetic characterization, ginsenoside analysis and micropropagation of American ginseng (Panax quinquefolius L.)." Morgantown, W. Va. : [West Virginia University Libraries], 2010. http://hdl.handle.net/10450/11231.

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Thesis (Ph. D.)--West Virginia University, 2010.<br>Title from document title page. Document formatted into pages; contains ix, 160 p. : ill. (some col.), col. maps. Includes abstract. Includes bibliographical references.

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Saka, Kamel. "REGENERATION OF COTTON (GOSSYPIUM HIRSUTUM L.) CALLUS PROTOPLASTS TO MACROCALLI." Thesis, The University of Arizona, 1985. http://hdl.handle.net/10150/275376.

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Tsoktouridis, Georgios. "Molecular detection and characterisation of bacteria intimately associated with Billbergia magnifica ssp. acutisepalia during micropropagation by axillary shoot proliferation and somatic embryogenesis." Thesis, Imperial College London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368912.

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Gribble, Karleen D., of Western Sydney Hawkesbury University, and Faculty of Science and Technology. "Towards an understanding of the physiological abnormality of tissue cultured plants known as vitrification." THESIS_FST_HPS_Gribble_K.xml, 1999. http://handle.uws.edu.au:8081/1959.7/417.

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For this research, the abnormality of tissue cultured plantlets,vitrification, was examined in Gypsophila paniculata.Measurement of the relative water content and water saturation deficit of plantlets in culture revealed that vitrified plantlets contain relatively more water and less air spaces than non-vitrified plantlets.The effect of relative humidity on vitrification and growth was investigated using a variety of methods.From the results found, it was determined the defining characteristic of vitrified plantlets is water filled intercellular spaces. It was also determined that the primary cause of vitrification is high relative humidity resulting in a lack of transpiration in vitro but that other factors such as unbalanced mineral nutrition or high medium cytokinin can exacerbate vitrification.Further research in tissue culture may investigate the influence of relative humidity on plant growth and morphology, the mechanism by which plants exclude water from their intercellular spaces and refine in vitro tissue mineral analysis as a means by which critical mineral concentrations can be determined.<br>Doctor of Philosophy (PhD)

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Ahmed, Muhammad Faisal. "Studies on the tissue culture and potential for the development of a genetic transformation system for avocados (Persea americana Mill.) /." View thesis, 2002. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030728.134303/index.html.

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Parmenter, Kathleen S. "Developmental regulation of axillary meristem initiation /." [St. Lucia, Qld.], 2004. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe.pdf.

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許霖慶 and Lam-hing Hui. "Studies on explant regeneration and morphogenesis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1985. http://hub.hku.hk/bib/B3120692X.

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Hui, Lam-hing. "Studies on explant regeneration and morphogenesis /." [Hong Kong : University of Hong Kong], 1985. http://sunzi.lib.hku.hk/hkuto/record.jsp?B1231481X.

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