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Relevant bibliographies by topics / Total internal reflection fluorescence microscopy

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Academic literature on the topic 'Total internal reflection fluorescence microscopy'

Author: Grafiati

Published: 4 June 2021

Last updated: 18 February 2022

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Journal articles on the topic "Total internal reflection fluorescence microscopy"

1

Yildiz, Ahmet, and Ronald D. Vale. "Total Internal Reflection Fluorescence Microscopy." Cold Spring Harbor Protocols 2015, no. 9 (2015): pdb.top086348. http://dx.doi.org/10.1101/pdb.top086348.

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Denham, Simon, and Deborah Cutchey. "Total Internal Reflection Fluorescence (TIRF) Microscopy." Imaging & Microscopy 11, no. 2 (2009): 54–55. http://dx.doi.org/10.1002/imic.200990043.

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Kudalkar, Emily M., Trisha N. Davis, and Charles L. Asbury. "Single-Molecule Total Internal Reflection Fluorescence Microscopy." Cold Spring Harbor Protocols 2016, no. 5 (2016): pdb.top077800. http://dx.doi.org/10.1101/pdb.top077800.

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Uchida, Maho, Rosa R. Mouriño-Pérez, and Robert W. Roberson. "Total internal reflection fluorescence microscopy of fungi." Fungal Biology Reviews 24, no. 3-4 (2010): 132–36. http://dx.doi.org/10.1016/j.fbr.2010.12.003.

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Schmoranzer, Jan, Mark Goulian, Dan Axelrod, and Sanford M. Simon. "Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence Microscopy." Journal of Cell Biology 149, no. 1 (2000): 23–32. http://dx.doi.org/10.1083/jcb.149.1.23.

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Total internal reflection fluorescence microscopy has been applied to image the final stage of constitutive exocytosis, which is the fusion of single post-Golgi carriers with the plasma membrane. The use of a membrane protein tagged with green fluorescent protein allowed the kinetics of fusion to be followed with a time resolution of 30 frames/s. Quantitative analysis allowed carriers undergoing fusion to be easily distinguished from carriers moving perpendicularly to the plasma membrane. The flattening of the carriers into the plasma membrane is seen as a simultaneous rise in the total, peak, and width of the fluorescence intensity. The duration of this flattening process depends on the size of the carriers, distinguishing small spherical from large tubular carriers. The spread of the membrane protein into the plasma membrane upon fusion is diffusive. Mapping many fusion sites of a single cell reveals that there are no preferred sites for constitutive exocytosis in this system.

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Beaumont, V. "Visualizing membrane trafficking using total internal reflection fluorescence microscopy." Biochemical Society Transactions 31, no. 4 (2003): 819–23. http://dx.doi.org/10.1042/bst0310819.

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There is a dizzying array of fluorescent probes now commercially available to monitor cellular processes, and advances in molecular biology have highlighted the ease with which proteins can now be labelled with fluorophores without loss of functionality. This has led to an explosion in the popularity of fluorescence microscopy techniques. One such specialized technique, total internal reflection fluorescence microscopy (TIR-FM), is ideally suited to gaining insight into events occurring at, or close to, the plasma membrane of live cells with excellent optical resolution. In the last few years, the application of TIR-FM to membrane trafficking events in both non-excitable and excitable cells has been an area of notable expansion and fruition. This review gives a brief overview of that literature, with emphasis on the study of the regulation of exocytosis and endocytosis in excitable cells using TIR-FM. Finally, recent applications of TIR-FM to the study of cellular processes at the molecular level are discussed briefly, providing promise that the future of TIR-FM in cell biology will only get brighter.

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Burghardt, Thomas P., Andrew D. Hipp, and Katalin Ajtai. "Around-the-objective total internal reflection fluorescence microscopy." Applied Optics 48, no. 32 (2009): 6120. http://dx.doi.org/10.1364/ao.48.006120.

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Axelrod, Daniel. "Total Internal Reflection Fluorescence Microscopy in Cell Biology." Traffic 2, no. 11 (2001): 764–74. http://dx.doi.org/10.1034/j.1600-0854.2001.21104.x.

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Kuznetsova, O. B., E. A. Savchenko, A. A. Andryakov, E. Y. Savchenko, and Z. A. Musakulova. "Image processing in total internal reflection fluorescence microscopy." Journal of Physics: Conference Series 1236 (June 2019): 012039. http://dx.doi.org/10.1088/1742-6596/1236/1/012039.

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Asbury, Charles L. "Data Analysis for Total Internal Reflection Fluorescence Microscopy." Cold Spring Harbor Protocols 2016, no. 5 (2016): pdb.prot085571. http://dx.doi.org/10.1101/pdb.prot085571.

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Dissertations / Theses on the topic "Total internal reflection fluorescence microscopy"

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Beck, Markus. "Extended resolution in total internal reflection fluorescence microscopy /." Zürich : ETH, 2008. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17974.

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Steele, Bridgett L. Thompson Nancy L. "Total internal reflection fluorescence microscopy for characterizing biochemical interactions." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2009. http://dc.lib.unc.edu/u?/etd,2725.

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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2009.<br>Title from electronic title page (viewed Mar. 10, 2010). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Chemistry." Discipline: Chemistry; Department/School: Chemistry.

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Chan, Ho Man. "Analysis of biomolecules by total internal reflection fluorescence microscopy." HKBU Institutional Repository, 2011. http://repository.hkbu.edu.hk/etd_ra/1254.

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Bethea, Tomika R. C. "Silica Colloidal Crystals as Porous Substrates for Total Internal Reflection Fluorescence Microscopy." Thesis, The University of Arizona, 2006. http://hdl.handle.net/10150/193371.

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In cell biology and chemistry, total internal reflection microscopy (TIRFM) has proven to be a useful technique that allows the probing of cellular processes with high-signal-to-noise ratio imaging. However, samples on solid substrates limit the accessibility to probe processes on extracellular membrane surface closest to the microscope objective. Colloidal crystals provide a porous alternative to the traditional solid substrates. Thin crystals exhibit optical properties similar to that of a fused silica coverslip allowing for TIRFM in the same manner as with a typical coverslip as demonstrated by the observance of Chinese hamster ovary cells with fluorescently labeled receptors on both types of substrates. Accessibility of the cell membrane closest to the substrate and the ability to probe fluorophore orientation information was observed by the binding of TIPP-cy5 to the human delta opioid receptor.

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Chan, Hei Nga. "Analysis of biomarkers of age-related diseases by total internal reflection fluorescence microscopy." HKBU Institutional Repository, 2018. https://repository.hkbu.edu.hk/etd_oa/527.

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Total internal reflection fluorescence microscopy (TIRFM) has been widely applied for the study of biomolecules because of their ability to quantify biomolecules in a sample pretreatment and enrichment free manner, when compared with those costly, sample consuming and labor intensive conventional detection assay. Here, we have applied the TIRFM imaging system for the direct quantification and analysis of the biomarkers for the age-related diseases. Three research works on the quantification and study of biomarkers with the aid of TIRFM were herein described.

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Ogden, Melinda Anne. "Two-photon total internal reflection microscopy for imaging live cells with high background fluorescence." Thesis, Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/34786.

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Fluorescence microscopy allows for spatial and temporal resolution of systems which are inherently fluorescent or which can be selectively labeled with fluorescent molecules. Temporal resolution is crucial for imaging real time processes in living samples. A common problem in fluorescence microscopy of biological samples is autofluorescence, fluorescence inherent to the system, which interferes with detection of fluorescence of interest by decreasing the signal to noise ratio. Two current methods for improved imaging against autofluorescence are two-photon excitation and total internal reflection microscopy. Two-photon excitation occurs when two longer wavelength photons are absorbed quasi-simultaneously by a single fluorophore. For this to take place there must be a photon density on the order of 1030 photons/(cm2)(s), which is achieved through use of a femtosecond pulsed laser and a high magnification microscope objective. Two-photon excitation then only occurs at the focal spot, significantly reducing the focal volume and therefore background autofluorescence. The second method, total internal reflection, is based on evanescent wave excitation, which decreases exponentially in intensity away from the imaging surface. This allows for excitation of a thin (~200 nm) slice of a sample. Since only a narrow region of interest is excited, an optical slice can be imaged, decreasing excitation of out-of-focus autofluorescence, and increasing the signal to noise ratio. By coupling total internal reflection with two-photon excitation, an entire cell can be imaged while still maintaining the use of lower energy photons to irradiate the sample and achieve two-photon excitation along the length traveled by the evanescent wave. This system allows for more sensitive detection of fluorescence of interest from biological systems as a result of a significant decrease in excitation volume and therefore a decrease in autofluorescence signal. In the two-photon total internal reflection microscopy setup detailed in this work, an excitation area of 20 μm by 30 μm is achieved, and used to image FITC-stained actin filaments in BS-C-1 cells

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Suda-Cederquist, Keith David. "Near-Wall Thermometry via Total Internal Reflection Fluorescence Micro-Thermometry (TIR-FMT)." Thesis, Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/14530.

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To effectively design systems of microchannels it is necessary for scientists and engineers to understand thermal transport characteristics of microchannels. To experimentally determine the convective heat transfer coefficient of microchannels it is necessary to measure both the bulk and surface temperature fields. This investigation aims to develop a technique, named Total Internal Reflection Fluorescent Micro-Thermometry (TIR-FMT), to measure the temperature of water within several hundred nanometers of a wall--effectively, the surface temperature of the wall. In TIR-FMT, an evanescent-wave is generated in the water near the wall. The intensity of this evanescent-wave decays exponentially with distance from the wall. A fluorophore if illuminated by the evanescent-wave can absorb a photon. Excited fluorophores subsequently emit red-shifted photons, which are called fluorescence. The probability of a fluorescent emission is temperature-dependent. Therefore, by monitoring the intensity of the fluorescence a correlation can be made to the temperature of the region of illumination. Using the TIR-FMT technique the temperature dependence of the fluorescence intensity from buffered fluorescein (pH=9.2) was determined to be 1.35%/C. TIR-FMT can be used to measure the temperature of a fluorophore solution within 600 nm of a wall across a temperature range of 12.5-55C. The average uncertainties (95% confidence) of the temperature measured was determined to be 2.3C and 1.5C for a single 1.5x1.5 and #956;m pixel and the entire 715x950 and #956;m viewfield. By spatial averaging, average uncertainties of 2.0C and 1.8C were attained with spatial resolutions of 16x16 and 100x100 and #956;m, respectively.

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Kwok, Ka Cheung. "Measuring binding kinetics of ligands with tethered receptors by fluorescence polarization complemented with total internal reflection fluorescence microscopy." HKBU Institutional Repository, 2010. https://repository.hkbu.edu.hk/etd_oa/18.

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The study of the binding between estrogen receptors (ER) and their ligands in vitro has long been of interest mainly because of its application in anti-estrogen drug discovery for breast cancer treatment as well as in the screening of environmental contaminants for endocrine disruptors. Binding strength was conventionally quantified in terms of equilibrium dissociation constant (KD). Recently, emphasis is shifting towards kinetics rate constants, and off-rate (koff) in particular. This thesis reported a novel method to measure such binding kinetics based on fluorescence polarization complemented with total internal reflection fluorescence (FP-TIRF). It used tethered receptors in a flow cell format. For the first time, the kinetics rate constants of the binding of full-length human recombinant ERα with its standard ligands were measured. koff was found to range from 1.3 10-3 to 2.3 10-3 s-1. kon ranged from 0.3 105 to 11 105 M-1 s-1. The method could also be used to screen potential ligands. Motivated by recent findings that ginsenosides might be functional ligands of nuclear receptors, eleven ginsenosides were scanned for binding with ER and peroxisome proliferator-activated receptor gamma (PPAR). None of the ginsenosides showed significant binding to ER, but Rb1 and 20(S)-Rg3 exhibited significant specific binding with PPAR.

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Kaldaras, Leonora. "Single Molecule Studies of Enzymes Horseradish Peroxidase and Alkaline Phosphatase Using Total Internal Reflection Fluorescence Microscopy and Confocal Microscopy." Bowling Green State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1374686174.

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Yiu, Kwok Wing. "Measuring the binding between estrogen receptor alpha and potential endocrine disruptors by fluorescence polarization and total internal reflection fluorescence." HKBU Institutional Repository, 2013. http://repository.hkbu.edu.hk/etd_ra/1503.

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Books on the topic "Total internal reflection fluorescence microscopy"

1

Parsons, David. The use of total internal reflection fluorescence spectroscopy to study polymer and particle adsorption. University of Salford, 1991.

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Book chapters on the topic "Total internal reflection fluorescence microscopy"

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Axelrod, Daniel. "Total Internal Reflection Fluorescence Microscopy." In Methods in Cellular Imaging. Springer New York, 2001. http://dx.doi.org/10.1007/978-1-4614-7513-2_21.

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Saxena, Anuj, Vishesh Dubey, Veena Singh, Shilpa Tayal, and Dalip Singh Mehta. "Field Portable Total Internal Reflection Fluorescence Microscopy." In Springer Proceedings in Physics. Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-9259-1_66.

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Wazawa, Tetsuichi, and Masahiro Ueda. "Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience." In Microscopy Techniques. Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/b102211.

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Axelrod, Daniel. "Total Internal Reflection Fluorescence Microscopy for Single-Molecule Studies." In Encyclopedia of Biophysics. Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-642-35943-9_479-1.

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Wasserstrom, Sebastian, Björn Morén, and Karin G. Stenkula. "Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7507-5_12.

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Axelrod, Daniel. "Total Internal Reflection Fluorescence Microscopy for Single-Molecule Studies." In Encyclopedia of Biophysics. Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-16712-6_479.

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Schwarz, Juliane P., Ireen König, and Kurt I. Anderson. "Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-207-6_25.

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Burchfield, James G., Jamie A. Lopez, and William E. Hughes. "Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualise Insulin Action." In Visualization Techniques. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-897-9_5.

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Nedzved, Olga, Luhong Jin, Alexander Nedzved, Wanni Lin, Sergey Ablameyko, and Yingke Xu. "Automatic Analysis of Moving Particles by Total Internal Reflection Fluorescence Microscopy." In Communications in Computer and Information Science. Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-35430-5_19.

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Senju, Yosuke, and Shiro Suetsugu. "Spatiotemporal Analysis of Caveolae Dynamics Using Total Internal Reflection Fluorescence Microscopy." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0732-9_6.

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Conference papers on the topic "Total internal reflection fluorescence microscopy"

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Gu, Min, and James W. M. Chon. "Scanning total internal reflection fluorescence microscopy." In 19th Congress of the International Commission for Optics: Optics for the Quality of Life, edited by Giancarlo C. Righini and Anna Consortini. SPIE, 2003. http://dx.doi.org/10.1117/12.529180.

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Dubey, Vishesh, Rajvinder Singh, Azeem Ahmad, Dalip Singh Mehta, and Balpreet Singh Ahluwalia. "Chip-based Total Internal Reflection Fluorescence Microscopy." In Frontiers in Optics. OSA, 2018. http://dx.doi.org/10.1364/fio.2018.fm4e.4.

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Bae, Soo Jin, Seung Yup Lee, and Uk Kang. "Fluorescence microscope using total internal reflection." In Biomedical Optics (BiOS) 2008, edited by Jörg Enderlein, Zygmunt K. Gryczynski, and Rainer Erdmann. SPIE, 2008. http://dx.doi.org/10.1117/12.764347.

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Chon, James W. M., and Min Gu. "Scanning total internal reflection fluorescence microscopy and its applications." In SPIE's International Symposium on Smart Materials, Nano-, and Micro- Smart Systems, edited by Dan V. Nicolau. SPIE, 2002. http://dx.doi.org/10.1117/12.476038.

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Guo, Min, Panagiotis Chandris, John P. Giannini, Jiji Chen, Harshad D. Vishwasrao, and Hari Shroff. "Combining Total Internal Reflection Fluorescence Microscopy with Rapid Super-resolution Imaging." In Novel Techniques in Microscopy. OSA, 2019. http://dx.doi.org/10.1364/ntm.2019.nw2c.4.

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Mudry, E., J. Girard, K. Belkebir, H. Giovannini, P. C. Chaumet, and A. Sentenac. "High-resolution total-internal-reflection fluorescence microscopy using periodically nanostructured glass slides." In Novel Techniques in Microscopy. OSA, 2011. http://dx.doi.org/10.1364/ntm.2011.nma4.

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Soubies, Emmanuel, Laure Blanc-Feraud, Sebastien Schaub, and Gilles Aubert. "Sparse reconstruction from Multiple-Angle Total Internal Reflection fluorescence Microscopy." In 2014 IEEE International Conference on Image Processing (ICIP). IEEE, 2014. http://dx.doi.org/10.1109/icip.2014.7025575.

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Wang, Jinyuan, Guo Fu, Chen Wang, Li Liu, and Guiying Wang. "Single dye molecules observed by total internal reflection fluorescence microscopy." In SPIE Proceedings, edited by Qingming Luo, Lihong V. Wang, Valery V. Tuchin, and Min Gu. SPIE, 2007. http://dx.doi.org/10.1117/12.741569.

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Bai, Yongqiang, Aihui Tang, Shiqiang Wang, and Xing Zhu. "Visualizing substructure of Ca2+waves by total internal reflection fluorescence microscopy." In Photonics Asia 2004, edited by Xing Zhu, Stephen Y. Chou, and Yasuhiko Arakawa. SPIE, 2005. http://dx.doi.org/10.1117/12.580734.

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Kim, Kyujung, Dong Jun Kim, Eun-Jin Cho, Yong-Min Huh, Jin-Suck Seo, and Donghyun Kim. "Nano-Grating-Based Plasmon Enhancement in Total Internal Reflection Fluorescence Microscopy." In Optical Fabrication and Testing. OSA, 2008. http://dx.doi.org/10.1364/oft.2008.jwd39.

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