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Journal articles on the topic 'Total viable bacterial'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Muñoz-Elías, Ernesto J., Juliano Timm, Tania Botha, Wai-Tsing Chan, James E. Gomez, and John D. McKinney. "Replication Dynamics of Mycobacterium tuberculosis in Chronically Infected Mice." Infection and Immunity 73, no. 1 (2005): 546–51. http://dx.doi.org/10.1128/iai.73.1.546-551.2005.

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ABSTRACT The dynamics of host-pathogen interactions have important implications for the design of new antimicrobial agents to treat chronic infections such as tuberculosis (TB), which is notoriously refractory to conventional drug therapy. In the mouse model of TB, an acute phase of exponential bacterial growth in the lungs is followed by a chronic phase characterized by relatively stable numbers of bacteria. This equilibrium could be static, with little ongoing replication, or dynamic, with continuous bacterial multiplication balanced by bacterial killing. A static model predicts a close corr

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Carol, Okoli, M.C Okonji, S.C Ugoh, S.N Okolo, A.C Okoli, and A.J Alu. "SALMONELLA SPECIE AND TOTAL VIABLE BACTERIAL LOAD IN ROASTED CHICKENS SOLD IN JOS-NIGERIA." Continental J. Biomedical Sciences 1 (June 18, 2007): 11–15. https://doi.org/10.5281/zenodo.818666.

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The study was to investigate for the presence of <em>Salmonella</em> specie and total viable aerobic bacterial load in roasted chickens sold in Jos. The study was carried out on twenty five chicken samples. No salmonella specie was isolated from the samples. However, other bacterial organisms were isolates, viz: 9(36%) of the samples yielded <em>E.coli</em>; 5(20%) yielded <em>Citobacter</em> species; 3(12%) yielded <em>Proteus </em>species and 6(24%) yielded <em>Klebsiella</em> species while 2(8%) showed no growth. An average total viable aerobic bacterial load of 7.9x10<sup>7</sup> organisms

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Stinson, Lisa F., Michelle L. Trevenen, and Donna T. Geddes. "The Viable Microbiome of Human Milk Differs from the Metataxonomic Profile." Nutrients 13, no. 12 (2021): 4445. http://dx.doi.org/10.3390/nu13124445.

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Bacteria in human milk contribute to the establishment of the infant gut microbiome. As such, numerous studies have characterized the human milk microbiome using DNA sequencing technologies, particularly 16S rRNA gene sequencing. However, such methods are not able to differentiate between DNA from viable and non-viable bacteria. The extent to which bacterial DNA detected in human milk represents living, biologically active cells is therefore unclear. Here, we characterized both the viable bacterial content and the total bacterial DNA content (derived from viable and non-viable cells) of fresh

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Oladele, Paul, and Timothy A. Johnson. "PSV-12 Evaluating the effect of lyoprotectants in preserving community structure and bacterial viability in the lyophilized fecal community of pigs." Journal of Animal Science 102, Supplement_3 (2024): 511. http://dx.doi.org/10.1093/jas/skae234.578.

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Abstract Fecal microbiota transplantation (FMT) stands as a potential method for enhancing pig health. FMT has progressed from the basic delivery of fresh stool, to lyophilized preparations which is beneficial for sample storage, but can damage bacterial cells. This has necessitated the need for lyoprotectants to preserve bacterial viability. However, the ideal lyoprotectant for retaining community structure and viability remains unclear. Furthermore, DNA-based microbial profiling methods such as 16S rRNA and metagenomics sequencing do not distinguish live and dead bacteria, prompting the need

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Ahmed Mohamed Moawad Mabrouk, Ahmed Mohamed Moawad Mabrouk. "Assessment of the Microbial Occupational Work Environment in One Faculty of King Abdulaziz University in Jeddah." journal of King Abdulaziz University - Meteorology, Environment and Arid Land Agriculture Sciences 26, no. 2 (2015): 75–82. http://dx.doi.org/10.4197/met.26-2.8.

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The presence of microorganisms in indoor air can pose serious health problems so; the purpose of the present work was to monitor the different groups of microorganisms including total viable bacteria, Staphylococci, total coliforms, Salmonella and fungi in different sites of the occupational environment (laboratories, offices, corridors and toilets) inside the Faculty of Metrology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia. The results showed that, the average of total viable bacterial counts in different work laboratories was ranged from 9500 to 23

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Sawaya, K., N. Kaneko, K. Fukushi, and J. Yaguchi. "Behaviors of physiologically active bacteria in water environment and chlorine disinfection." Water Science and Technology 58, no. 7 (2008): 1343–48. http://dx.doi.org/10.2166/wst.2008.445.

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Direct microscopic methods using several fluorescent staining were applied to estimate the proportion of physiologically active bacteria in the water environment and evaluate the efficacy of disinfection with chlorine. 4′,6-diamidino-2-phenylindole (DAPI) was used to determine total bacterial numbers, and 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was chosen for direct detection of respiring bacteria. BacLightTM kit was used to assess bacterial membrane integrity. Bacteria with growth potential were enumerated using the DVC method and microcolony technique. The total bacterial number in ri

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Sansupa, Chakriya, Sara Fareed Mohamed Wahdan, Terd Disayathanoowat, and Witoon Purahong. "Identifying Hidden Viable Bacterial Taxa in Tropical Forest Soils Using Amplicon Sequencing of Enrichment Cultures." Biology 10, no. 7 (2021): 569. http://dx.doi.org/10.3390/biology10070569.

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This study aims to estimate the proportion and diversity of soil bacteria derived from eDNA-based and culture-based methods. Specifically, we used Illumina Miseq to sequence and characterize the bacterial communities from (i) DNA extracted directly from forest soil and (ii) DNA extracted from a mixture of bacterial colonies obtained by enrichment cultures on agar plates of the same forest soil samples. The amplicon sequencing of enrichment cultures allowed us to rapidly screen a culturable community in an environmental sample. In comparison with an eDNA community (based on a 97% sequence simil

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Mahmoud, M. Abo-El-Saad, E. I. Badawy Mohamed, and M. M. Mohammed Youssef. "ANALYSIS AND IDENTIFICATION OF MICROBIAL SPECIES ASSOCIATED WITH THE EGYPTIAN DATE FRUITS DURING POSTHARVEST STORAGE." Journal of Applied Biological Sciences 17, no. 1 (2023): 138–54. https://doi.org/10.5281/zenodo.7579923.

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Date fruits are consumed in many countries globally and are mostly loaded with a mixture of microbes. As a result, various bacterial and fungal species attack them, thus causing their spoilage at the ripening, storage, and processing stages. The present study investigates the current spoilage bacteria and fungi of twelve postharvest date fruits collected from different Egyptian geographical regions (Aswan, Siwa, and El-Wady El-Gadeed). Isolation and identification of date-borne microbes based on morphological and molecular identification were studied. The microscopic examination of infected da

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Stinson, Lisa F., Michelle L. Trevenen, and Donna T. Geddes. "Effect of Cold Storage on the Viable and Total Bacterial Populations in Human Milk." Nutrients 14, no. 9 (2022): 1875. http://dx.doi.org/10.3390/nu14091875.

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Expression and cold storage of human milk is a common practice. Current guidelines for cold storage of expressed milk do not take into account the impact on the milk microbiome. Here, we investigated the impact of cold storage on viable bacterial populations in human milk. Freshly expressed milk samples (n = 10) were collected and analysed immediately, stored at 4 °C for four days, −20 °C for 2.25 months and 6 months, and −80 °C for 6 months. Samples were analysed using propidium monoazide (PMA; a cell viability dye) coupled with full-length 16S rRNA gene. An aliquot of each sample was additio

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Gitau, George, Daniel Muasya, Willy Mwangi, et al. "Risk factors associated with camel milk total coliform and total viable bacterial count along the camel milk value chain in Isiolo County, Kenya." Journal of Research in Veterinary Sciences 5, no. 1 (2025): 29. https://doi.org/10.5455/jrvs.20241215111750.

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Abstract Background and Aim: The camel is an important livestock in the semi-arid, arid and desert regions. The study was carried out to determine the level of bacterial contamination and factors associated with total bacterial count and coliform contamination along the camel milk chain in Isiolo County, Kenya. Materials and Methods: A cross-sectional study was carried out between February and March 2024 and milk samples were collected along the milk value chain. Milk samples were collected from farmers, transporters, milk bulkers, and retailers. The samples were assessed for total viable bact

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Ding, Yun, and Yong Guang Yin. "A close to Real-Time Intelligent System for Enumerating Total Viable Bacteria Based on Pattern Recognition Technology." Applied Mechanics and Materials 741 (March 2015): 344–49. http://dx.doi.org/10.4028/www.scientific.net/amm.741.344.

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In this paper, a novel close to real-time artificial intelligent system for enumerating Total Viable Bacteria (TVB) in drinking water was developed by using pattern recognition and machine vision technology. In order to identify the viable bacteria accurately, four shape features including circularity ratio, eccentricity, rectangularity, and compact degree, and four color features (GRsd, BRsd, HRsd, SRsd) of the stained viable bacteria image were extracted. An optimal artificial neural network was used as the bacterial recognition classifier, whose inputs were the extracted feature parameters

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Newbold, C. J., F. M. McIntosh, and R. J. Wallace. "Changes in the microbial population of a rumen-simulating fermenter in response to yeast culture." Canadian Journal of Animal Science 78, no. 2 (1998): 241–44. http://dx.doi.org/10.4141/a97-086.

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Yeast increased the total viable count of bacteria on a medium containing rumen fluid and soluble sugars by 38% (P < 0.05) and the cellulolytic population increased by 49%, although the increase was not statistically significant (P > 0.05). Microscopic counts indicated a 25% increase in total bacteria (P < 0.05). Bacterial counts with mannitol as sole energy source also increased (P < 0.05), indicating an increased Selenomonas population; that was not in itself sufficient to account for the increase in the total bacterial count. The protozoal population was significantly lower in y

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HUTCHISON, M. L., L. D. WALTERS, G. C. MEAD, M. HOWELL, and V. M. ALLEN. "An Assessment of Sampling Methods and Microbiological Hygiene Indicators for Process Verification in Poultry Slaughterhouses." Journal of Food Protection 69, no. 1 (2006): 145–53. http://dx.doi.org/10.4315/0362-028x-69.1.145.

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Studies to determine the appropriateness of the use of populations of indicator bacteria on poultry carcasses for process verification were undertaken in commercial slaughterhouses. Samples were collected from neck skin by excision or from whole carcass rinses and were examined for a range of presumptive process hygiene indicator bacteria. Coefficients of variation were calculated for each bacterial indicator and were significantly lower in excised samples, indicating more reproducible bacterial recovery by this sampling method. Total viable counts of aerobic bacteria, Enterobacteriaceae, and

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Odo, Joel Inya, Ebah EE, and Eberechukwu AM. "Bacterial Contamination of Drinking Wells in Makurdi Metropolis." International Journal of Oceanography & Aquaculture 8, no. 2 (2024): 1–9. http://dx.doi.org/10.23880/ijoac-16000307.

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This study was carried out to assess the bacterial contamination of drinking wells in Makurdi metropolis. Fifteen (15) untreated water samples, five (5) each were collected from wells in High Level, Wurukum and Wadata areas for physicochemical and bacteriological analysis was done using pour plating and the bacteriological quality was assessed by measuring the total viable, total coliform and fecal coliform counts of bacteria in the samples using Mac Conkey agar and Nutrient agar. The results of the analysis showed that the pH ranged from 6.70 ± 0.16 to 7.40± 0.87, the temperature levels range

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JI, Odo. "Bacterial Contamination of Drinking Wells in Makurdi Metropolis." Open Access Journal of Microbiology & Biotechnology 9, no. 2 (2024): 1–9. http://dx.doi.org/10.23880/oajmb-16000295.

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This study was carried out to assess the bacterial contamination of drinking wells in Makurdi metropolis. Fifteen (15) untreated water samples, five (5) each were collected from wells in High Level, Wurukum and Wadata areas for physicochemical and bacteriological analysis was done using pour plating and the bacteriological quality was assessed by measuring the total viable, total coliform and fecal coliform counts of bacteria in the samples using Mac Conkey agar and Nutrient agar. The results of the analysis showed that the pH ranged from 6.70 ± 0.16 to 7.40± 0.87, the temperature levels range

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Kyoui, Daisuke, Yuka Saito, Akifumi Takahashi, et al. "Antibacterial Activity of Hexanol Vapor In Vitro and on the Surface of Vegetables." Foods 12, no. 16 (2023): 3097. http://dx.doi.org/10.3390/foods12163097.

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Hexanol is a volatile alcohol and a major component of plant essential oils (EOs). However, the antibacterial activity of hexanol vapor has not been well studied. This study aimed to evaluate the antibacterial activity of hexanol. In this study, seven food-related bacteria were exposed to 1-, 2- or 3-hexanol vapor on agar media to evaluate their growth. Additionally, the total viable counts in three vegetables when exposed to 1-hexanol vapor were measured. The results showed that 1-hexanol exhibited antibacterial effects against Gram-negative bacteria but did not affect Gram-positive bacteria.

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Sjollema, Jelmer, Minie Rustema-Abbing, Henny C. van der Mei, and Henk J. Busscher. "Generalized Relationship between Numbers of Bacteria and Their Viability in Biofilms." Applied and Environmental Microbiology 77, no. 14 (2011): 5027–29. http://dx.doi.org/10.1128/aem.00178-11.

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ABSTRACTBacterial biofilms are confined communities that are encapsulated in protective layers of extracellular polymeric substances. Microscopic evaluation of biofilms of diverse bacterial strains on various substrata reveals that, in general, the percentage of viable bacteria decreases with the total number of bacteria in a biofilm.

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Haque, Md Mansurul, MG Sorrowar, and Harun Ur Rashid. "Effects of frozen storage, radiation and their combined Treatments on microorganisms of freshwater mola fish Amblypharyngodon mola." Journal of Bangladesh Academy of Sciences 37, no. 1 (2013): 21–31. http://dx.doi.org/10.3329/jbas.v37i1.15677.

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The study revealed that the total viable bacterial count, staphylococcal count, coliform count, faecal coliform count, Aeromonas count and total fungal counts varied from 8.8×107 to 1.27×108, 2.7×106 to 4.2×106, 9.0×104 to 1.03×105, 4.8×105 to 7.3×105, 2.7×105 to 5.0×105 and 1.6×103 to 3.5×103 cfu/gm, respectively. Seventy six bacterial strains were isolated and identified, where Staphylococcus aureus, Micrococcus varians, Aeromonas hydrophila, Klebsiella ozaenae, Bacillus subtilis, Escherichia coli, Bacillus megaterium, Klebsiella edwardsii, Pseudomonas aerugenosa and Micrococcus radiodurans

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Bogomolny, E. "Correction: Total viable bacterial count using a real time all-fibre spectroscopic system." Analyst 141, no. 15 (2016): 4804. http://dx.doi.org/10.1039/c6an90038k.

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Asiya, P., Manjula Menon, Girigan Gopi, and Vipin Das. "Impact of Cropping Systems on Soil Microbial Load: Evidence from Wetland Ecosystems of Wayanad District, Kerala." Mapana - Journal of Sciences 18, no. 4 (2019): 1–8. http://dx.doi.org/10.12723/mjs.51.1.

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This study enumerated the microbial communities in soil samples from rice, banana and arecanut from the wet lands of Wayanad district Kerala. The total viable bacterial count in the paddy field was 120x10-6 cfu, 121x10-6 cfu and 147x10-6 cfu in Nenmeni, Kaniyambatta and Pozhuthana Gramapanchayat and that of fungi was 30x10-3 cfu, 32 x10-3 cfu and 37 x10-3 cfu. Likewise, the total viable count of bacteria in areca nut at Nenmeni, Kaniyambatta and Pozhuthana Gramapanchayat was 66 x10-6 cfu, 80 x10-6 cfu, 118 x10-6 cfu and that of fungi was 14 x10-3 cfu, 18 x10-3 cfu, and 30 x10-3 cfu. The total

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Horve, Patrick F., Leslie G. Dietz, Suzanne L. Ishaq, Jeff Kline, Mark Fretz, and Kevin G. Van Den Wymelenberg. "Viable bacterial communities on hospital window components in patient rooms." PeerJ 8 (July 27, 2020): e9580. http://dx.doi.org/10.7717/peerj.9580.

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Previous studies demonstrate an exchange of bacteria between hospital room surfaces and patients, and a reduction in survival of microorganisms in dust inside buildings from sunlight exposure. While the transmission of microorganisms between humans and their local environment is a continuous exchange which generally does not raise cause for alarm, in a hospital setting with immunocompromised patients, these building-source microbial reservoirs may pose a risk. Window glass is often neglected during hospital disinfection protocols, and the microbial communities found there have not previously b

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Hoshi, Masakazu, Hideaki Nishi, Tetsuhito Hayashi, Masayo Okuzumi, and Etsuo Watanabe. "Development of biosensor for the determination of total viable bacterial cell count." NIPPON SUISAN GAKKAISHI 57, no. 2 (1991): 281–85. http://dx.doi.org/10.2331/suisan.57.281.

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Bogomolny, E., S. Swift, and F. Vanholsbeeck. "Total viable bacterial count using a real time all-fibre spectroscopic system." Analyst 138, no. 14 (2013): 4112–19. http://dx.doi.org/10.1039/c3an00254c.

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Hossain, Mohammad Zabed, Chaman Binta Aziz, and Mihir Lal Saha. "Relationships between soil physico-chemical properties and total viable bacterial counts in Sunderban mangrove forests, Bangladesh." Dhaka University Journal of Biological Sciences 21, no. 2 (2012): 169–75. http://dx.doi.org/10.3329/dujbs.v21i2.11515.

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Although soil bacterial communities are one of the important biotic components that influence decomposition and nutrient mineralization in the terrestrial ecosystems, factors driving this biotic community in the Sunderban mangrove forests are not well studied. The present study examined the importance of soil physico?chemical properties in driving soil bacterial communities in the Sunderban mangrove forests, Bangladesh. Soils were collected from 12 locations under four sites, namely Koromjal, Kotka, Hironpoint, and Dublarchar of Sunderban forests. Results showed a large range of variation in t

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Sánchez, María C., Paola Hernández, Ángela Velapatiño, Eber Cuba, María J. Ciudad, and Luis Collado. "Illumina Sequencing in Conjunction with Propidium Monoazide to Identify Live Bacteria After Antiseptic Treatment in a Complex Oral Biofilm: A Study Using an Ex Vivo Supragingival Biofilm Model." Antibiotics 13, no. 11 (2024): 1000. http://dx.doi.org/10.3390/antibiotics13111000.

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Background/Objectives: The evaluation of the efficacy of antibacterial treatments in complex oral ecosystems is limited by the inability to differentiate live from dead bacteria using omic techniques. The objective of this study was therefore to assess the ability of the combination of the 16S rRNA Illumina sequencing methodology and the action of propidium monoazide (PMA) to study viable bacterial profiles in oral biofilms after exposure to an antiseptic compound. Methods: Cariogenic supragingival biofilms were developed in an ex vivo model for 96 h, using saliva from healthy volunteers. The

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Umeanaeto, P. U., E. G. Ani, V. I. Anyaoha, J. U. Anumba, S. C. Afulukwe, and C. C. Okoli. "Parasitic and bacterial contamination of sachets water sold at Ngwo, Enugu State, Nigeria." Nigerian Journal of Parasitology 43, no. 2 (2022): 303–10. http://dx.doi.org/10.4314/njpar.v43i2.13.

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Water is an essential part of human nutrition. It is readily available in sachets with little or no interest in the quality by the producers. This study focused on the isolation and identification of parasites and bacteria contaminating sachet water sold at Ngwo, Enugu State, Nigeria. Twenty-one sachets of water were collected randomly from seven different brands sold within the community. The parasites were identified using sedimentation technique while bacteria were isolated from water cultured in Nutrient, MacConkey, and Eosin Methylene Blue Agar media. The physicochemical parameters analys

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Dewanti, Andita Ratih, Anggi Octari Putri, Indah Istiqomah Istiqomah, and Alim Isnansetyo. "Safety, Adherence, Enzymatic Activities, and Application Effects of Oral Probiotic Candidates for Shortfin Eel (Anguilla bicolor bicolor)." Jurnal Ilmiah Perikanan dan Kelautan 14, no. 2 (2022): 203–13. http://dx.doi.org/10.20473/jipk.v14i2.34315.

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Highlight Research The shortfin eel elver bicolor bicolor was tested for the safety of Enterobacter sp. JC05, Lactococcus sp. JAL37, and Bacillus sp. PCP1 The ability of bacterial strains to adhere to epithelial cells of shortfin eel epithelial cells was demonstrated The bacterial strains' proteolytic, cellulolytic, and lipolytic activities were detected Oral administration of the bacterial cocktail lowered overall viable bacterial count but did not affect the shortfin eel's intestinal histological characteristics Abstract Aquaculture of the shortfin eel (Anguilla bicolor bicolor) has been pla

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Wang, Xiaoyang, Ruohan Liang, Xiaodan Pu, et al. "Application of the Electrical Microbial Growth Analyzer Method for Efficiently Quantifying Viable Bacteria in Ready-to-Eat Sea Cucumber Products." Microorganisms 12, no. 11 (2024): 2301. http://dx.doi.org/10.3390/microorganisms12112301.

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Accurate and efficient quantification of viable bacteria in ready-to-eat food products is crucial for food safety and public health. The rapid and accurate assessment of foodborne bacteria in complex food matrices remains a significant challenge. Herein a culture-based approach was established for easily quantifying viable bacteria in ready-to-eat sea cucumber (RSC) products. Samples of the liquid companion within the package were directly transferred into test tubes to determine bacterial growth curves and growth rate curves, utilizing the electrical microbial growth analyzer. Viable bacteria

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Ahmed, Egbal Osman, and Mohamed Eltaher Ali. "Effect of the Seasons and Salt Concentrations on Microbial Load of Wet-Salted Fermented Product (Fassiekh)." Al-Mukhtar Journal of Sciences 32, no. 1 (2017): 46–53. http://dx.doi.org/10.54172/mjsc.v32i1.93.

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The study aims at investigating the microbial load of wet-salted fermented product (a traditional fermented product in Sudan named locally; fassiekh) and determination of total viable bacterial count, according to salt concentrations (20%, 25% and 30% of the fish weight) and seasons (summer, autumn and winter) variations. Fessiekh processed from popular fessiekh fish species (Hydrocynus spp, local name: Kass).The isolation and identification of bacteria and mould were examined for the microbial quality of fassiekh. The total viable count of bacteria in fresh fish used as raw materials in fessi

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Haidinger, W., M. P. Szostak, W. Jechlinger, and W. Lubitz. "Online Monitoring of Escherichia coli Ghost Production." Applied and Environmental Microbiology 69, no. 1 (2003): 468–74. http://dx.doi.org/10.1128/aem.69.1.468-474.2003.

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ABSTRACT Controlled expression of cloned φX174 gene E in gram-negative bacteria results in lysis of the bacteria by the formation of a transmembrane tunnel structure built through the cell envelope complex. Production of bacterial ghosts is routinely monitored by classical microbiological procedures. These include determination of the turbidity of the culture and the total number of cells and the number of reproductive cells present during the time course of growth and lysis. Although conceptually simple, these methods are labor intensive and time consuming, providing a complete set of results

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Cyprowski, Marcin, Anna Ławniczek-Wałczyk, Agata Stobnicka-Kupiec, Małgorzata Gołofit-Szymczak, and Rafał L. Górny. "Across-Shift Changes in Viable Nasal Bacteria among Waste-Incineration Plant Workers—A Pilot Study." International Journal of Environmental Research and Public Health 19, no. 15 (2022): 8984. http://dx.doi.org/10.3390/ijerph19158984.

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The aim of this pilot study was to assess the time-related changes in viable nasal bacteria concentrations among waste-incineration plant (WIP) workers compared to a group of office building (OB) workers outside the plant. In total, 20 volunteers participated in the study, including 14 WIP and 6 OB workers. WIP workers were divided into two sub-groups: supervisory staff (SVS) and maintenance and repair workers (MRW). Nasal swabs were collected before and after the morning work shift. Airborne bacteria were sampled with a six-stage impactor to assess the bioaerosol size distribution. The analys

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Kowalski, Michał, Jozef S. Pastuszka, Agnieszka Brąszewska, Josef Cyrys, and Ewa Brągoszewska. "Airborne Bacteria in Gliwice—The Industrialized City in Poland." Atmosphere 13, no. 10 (2022): 1721. http://dx.doi.org/10.3390/atmos13101721.

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The results of the study on the characteristics of the viable (culturable) and total bacterial particles in the ambient air in Gliwice, Poland, are presented. The concentration of viable bacteria in the air ranged from 57 CFU m−3 (Colony Forming Units per cubic meter) during winter to 305 CFU m−3 in spring, while the concentration of all bacteria (live and dead) in the air, measured in selected days, ranged from 298 cells m−3 in winter to over 25 thousand per m3 in autumn. A field study was also carried out to find out the level of the sterilization rate (k) for airborne bacteria. The obtained

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Enad, Hind H., and Mohammed Nahidh. "Salivary Cortisol as a Stress Biomarker and Total Viable Count of Salivary Bacterial Microbiome among COVID-19 Patients." Journal of Baghdad College of Dentistry 33, no. 4 (2021): 6–10. http://dx.doi.org/10.26477/jbcd.v33i4.3013.

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Background: The COVID-19 virus outbreak had a massive effect on many parts of people's lives, as they were advised to quarantine and lockdown to prevent the virus from spreading, which had a big impact on people's mental health, anxiety, and stress. Many internal and external factors lead to stress. This negatively influences the body's homeostasis. As a result, stress may affect the body's capacity to use energy to defend against pathogens. Many recent investigations have found substantial links between human mental stress and the production of hormones, prohormones, and/or immunological chem

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Luna, G. M., E. Manini, and R. Danovaro. "Large Fraction of Dead and Inactive Bacteria in Coastal Marine Sediments: Comparison of Protocols for Determination and Ecological Significance." Applied and Environmental Microbiology 68, no. 7 (2002): 3509–13. http://dx.doi.org/10.1128/aem.68.7.3509-3513.2002.

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ABSTRACT It is now universally recognized that only a portion of aquatic bacteria is actively growing, but quantitative information on the fraction of living versus dormant or dead bacteria in marine sediments is completely lacking. We compared different protocols for the determination of the dead, dormant, and active bacterial fractions in two different marine sediments and at different depths into the sediment core. Bacterial counts ranged between (1.5 ± 0.2) × 108 cells g−1 and (53.1 ± 16.0) × 108 cells g−1 in sandy and muddy sediments, respectively. Bacteria displaying intact membrane (liv

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Pratten, J., and M. Wilson. "Antimicrobial Susceptibility and Composition of Microcosm Dental Plaques Supplemented with Sucrose." Antimicrobial Agents and Chemotherapy 43, no. 7 (1999): 1595–99. http://dx.doi.org/10.1128/aac.43.7.1595.

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ABSTRACT The aims of this study were to evaluate the effects of repeated chlorhexidine gluconate (CHG) pulsing on the viability and bacterial composition of microcosm dental plaques derived from human saliva. The biofilms were grown on bovine enamel discs in a constant-depth film fermentor fed with an artificial saliva which was supplemented thrice daily with sucrose. The microcosm plaques had total viable anaerobic counts of 5 × 108 CFU per mm2 and consisted of 12% Actinomyces spp., 85% streptococci, and 0.2% Veillonella spp. When pulsed twice daily with 0.2% CHG, there was an immediate 1.3-l

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Helmi, K., F. Barthod, G. Méheut, et al. "Methods for microbiological quality assessment in drinking water: a comparative study." Journal of Water and Health 13, no. 1 (2014): 34–41. http://dx.doi.org/10.2166/wh.2014.056.

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The present study aimed to compare several methods for quantifying and discriminating between the different physiological states of a bacterial population present in drinking water. Flow cytometry (FCM), solid-phase cytometry (SPC), epifluorescence microscopy (MSP) and culture method performances were assessed by comparing the results obtained for different water samples. These samples, including chlorinated and non-chlorinated water, were collected in a drinking water treatment plant. Total bacteria were quantified by using SYBR Green II (for FCM) and 4′,6′-diamino-2-phenylindole (DAPI) (for

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Tong, Yingjia, Zeqing Wang, Qunyi Tong, and Yutong Liu. "Effects of Lactic Acid Bacteria Fermentation and In Vitro Simulated Digestion on the Bioactivities of Purple Sweet Potato Juice." Foods 13, no. 24 (2024): 4094. https://doi.org/10.3390/foods13244094.

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The effects of lactic acid bacteria fermentation and in vitro simulated digestion on phenolic bioavailability, phenolic bioavailability, and antioxidant activity of purple sweet potato juice (PSPJ) were investigated. The PSPJ was fermented by Lactobacillus rhamnosus and Streptococcus thermophilus. The viable bacterial count, phenolic components, antioxidant activity, phenolic bioaccessibility, and phenolic bioavailability of PSPJ were analyzed during the simulated digestion process in vitro. The data displayed that lactic acid bacteria fermentation increased total α-glucosidase inhibition, tot

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Alam, Nur E., Md Rayhan Ali, Md Tarek Molla, Shahin Mahmud, Kaisar Ali Talukder, and AKM Mohiuddin. "Therapeutic Potential of Plant Extracts Against Multidrug Resistance Poultry Bacteria." Plant Tissue Culture and Biotechnology 30, no. 1 (2020): 119–30. http://dx.doi.org/10.3329/ptcb.v30i1.47797.

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Plant extracts were evaluated on bacteria isolated from poultry farm for developing substitutive therapeutic agent of antibiotics. A diverse range of bacterial load observed both in total viable count (TVC) and in total coliform count (TCC) in 30 samples randomly collected from poultry feeds, drinking water and faeces. A total of six bacterial isolates e.g. Pseudomonas spp., Aeromonas spp., Citrobacter spp., Vibrio spp., Escherichia coli and Plesiomonas spp. were found in the samples cultured in MacConkey Agar medium. Fifteen antibiotics were studied against bacterial susceptibility. All the b

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Dauphin, Dighitoghi Moro. "Bacteriological investigation of meat sold in Ojo, Lagos, Nigeria." World Journal of Advanced Research and Reviews 10, no. 1 (2021): 136–42. https://doi.org/10.5281/zenodo.4733568.

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Meat (beef) is one of the most widely consumed food products which is remarkably known for its high protein content, vitamins and desirable taste. Meat is consumed by all and sundry. However, the intrinsic properties of meat make it highly susceptible to changes usually brought about by an array of physical, chemical and biochemical attributes. Several bacterial species have been implicated in the spoilage of meat and it provides a good source of nutrients. One kilogram of meat samples purchased from ten retail points in different markets namely Okoko, Ojo, Iyana-Iba, Igbo-Elerin, Ajangbadi, I

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Tian, Shaonan, Zhe Tian, Hong Yang, Min Yang, and Yu Zhang. "Detection of Viable Bacteria during Sludge Ozonation by the Combination of ATP Assay with PMA-Miseq Sequencing." Water 9, no. 3 (2017): 166. http://dx.doi.org/10.3390/w9030166.

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Using sludge obtained from municipal sewage treatment plants, the response of viable bacterial populations during the sludge ozonation process was investigated by a combination of adenosine triphosphate (ATP) assay and propidium monoazide (PMA)-Miseq sequencing. The ATP assay was first optimized for application on sludge samples by adjusting the sludge solid contents and reaction time. PMA-modified polymerase chain reaction (PCR) was also optimized by choosing the suitable final PMA concentration. The quantity and composition of viable bacterial populations during sludge ozonation were further

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D'Costa, Priya M., and Arga C. Anil. "Penicillin-mediated changes in viable benthic diatom assemblages – insights about the relevance of bacteria across spatial and seasonal scales." Marine and Freshwater Research 65, no. 5 (2014): 437. http://dx.doi.org/10.1071/mf13083.

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Penicillin, a β-lactam antibiotic, cannot affect diatoms directly but does so through bacteria. Its effects on viable benthic diatom assemblages in a tropical environment were evaluated across spatial (intertidal sandflat, mangrove site, port environment) and seasonal (post-monsoon, pre-monsoon, monsoon) scales. Penicillin treatment resulted in bacterial suppression and a reduction in diatoms at the intertidal and mangrove sites having stable pennate-dominated assemblages. Diatom response at the port site, dominated by transient centric diatoms, ranged from total inhibition to enhancement. The

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Nur, I. T., M. Talukder, T. R. Das, M. Asaduzzaman, F. Feroz, and S. K. Munshi. "Microbiological status of some street iftar items collected from chalk bazar in Dhaka city, Bangladesh." Food Research 5, no. 3 (2021): 236–40. http://dx.doi.org/10.26656/fr.2017.5(3).617.

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In Dhaka, the capital of Bangladesh and one of the most densely populated cities of the world, different categories of street foods are widely consumed by all classes of people, especially for iftar during the holy month of Ramadan. The objective of this research was to assess the microbiological quality of street iftar food items collected from a street in Chalk Bazar locality of Dhaka along with the antibiogram profile of the bacterial isolates. A total of 74 samples belong to ten different categories of street food items and 8 different types of street vended juices were collected aseptical

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Newbold, C. J., R. J. Wallace, and F. M. Mcintosh. "Mode of action of the yeastSaccharomyces cerevisiaeas a feed additive for ruminants." British Journal of Nutrition 76, no. 2 (1996): 249–61. http://dx.doi.org/10.1079/bjn19960029.

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Two suggested modes of action of yeast in stimulating rumen fermentation were investigated. The first, that yeast respiratory activity protects anaerobic rumen bacteria from damage by O2, was tested using different strains of yeast that had previously been shown to have differing abilities to increase the viable count of rumen bacteria.Saccharomyces cerevisiaeNCYC 240, NCYC 1026, and the commercial product Yea-Sacc®, added to rumen fluidin vitroat 1·3 mg/ml, increased the rate of O2disappearance by between 46 and 89%. The same three preparations also stimulated bacterial numbers in anin vitrof

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Hasan M, Hossain MM, Hasan MN, et al. "Seasonal variation of bacteriological parameters of rui (Labeo rohita) and kalibaus (Labeo calbasu) collected from the Hakaluki Haor, Bangladesh." Journal of Agriculture, Food and Environment 06, no. 01 (2025): 47–53. https://doi.org/10.47440/jafe.2025.6106.

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The fish in situbacterial flora correlated with the aquatic environment. The higher initial bacterial flora in fish ultimately affects the storage life and quality of the products. The present study aimed to collect 36 samples of Rui (Labeo rohita) and Kalibaus (Labeo calbasu) from three locations in four seasons (pre-monsoon, monsoon, post-monsoon and winter) of Hakaluki haor to investigate their seasonal bacteriological changes. The samples were investigated for total bacterial load in term of Total Viable Count (TVC) and Total Coliform Count (TCC) as sanitary index The prevalence of E. coli

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ONYANGO, S., H. PALMADOTTIR, T. TÓMASON, V. T. MARTEINSSON, P. M. K. NJAGE, and E. REYNISSON. "Influence of Thawing Methods and Storage Temperatures on Bacterial Diversity, Growth Kinetics, and Biogenic Amine Development in Atlantic Mackerel." Journal of Food Protection 79, no. 11 (2016): 1929–37. http://dx.doi.org/10.4315/0362-028x.jfp-16-094.

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ABSTRACT Limited knowledge is currently available on the influence of fish thawing and subsequent storage conditions on bacterial growth kinetics, succession, and diversity alongside the production of biogenic amines. This study aimed to address these factors during the thawing and subsequent storage of mackerel. Thawing was either done fast in 18°C water for 2 h or slowly at 30°C overnight. Subsequent storage was at 30°C (ambient) for 36 h and 2 to 5°C (refrigerated) for 12 days. The cultivation methods used were total viable counts, hydrogen sulfide–producing bacteria, and Pseudomonas. Maxim

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Leon, Ingrid M., Brent W. Auvermann, K. Jack Bush, et al. "Comprehensive Analysis of E. coli, Enterococcus spp., Salmonella enterica, and Antimicrobial Resistance Determinants in Fugitive Bioaerosols from Cattle Feedyards." Applied Microbiology 5, no. 3 (2025): 63. https://doi.org/10.3390/applmicrobiol5030063.

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Antimicrobial use in food animals selects for antimicrobial-resistant (AMR) bacteria, which most commonly reach humans via the food chain. However, AMR bacteria can also escape the feedyard via agricultural runoff, manure used as crop fertilizer, and even dust. A study published in 2015 reported AMR genes in dust from cattle feedyards; however, one of the study’s major limitations was the failure to investigate gene presence in viable bacteria, or more importantly, viable bacteria of importance to human health. Our main objective was to investigate the presence and quantity of viable bacteria

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Althulthi, Rihab Fouad, Raja Moftah Moman, Amnnah Abdullah Ghalbun, and Mohamed Ramadan Alsagher. "Evaluation of Bacteriological Quality and Preservatives Efficacy of Cosmetics." Scholars Academic Journal of Biosciences 11, no. 12 (2023): 442–51. http://dx.doi.org/10.36347/sajb.2023.v11i12.004.

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Cosmetics are external preparations normally applied to human body parts to enhance or alter the appearance of the face or fragrance and texture of the body. The common cosmetic products include: foundations, mascaras, powders, lipsticks, eye shadow, skin cleansers, body lotions, shampoos, hairstyling products (gel, hair spray, etc.), perfumes and colognes. Total of (96) cosmetic products were investigated. Twenty (20) samples of foundations, mascaras, compact powders, (5) samples of lip-gloss and (15) sponges of compact powder making a total of 80 products were bought from the market and eval

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Bhattarai, Rewati Raman, and Suman Kumar Lal Das. "Evaluation of Microbiological Quality of Indigenous Dahi from Eastern Nepal." Sunsari Technical College Journal 2, no. 1 (2016): 23–26. http://dx.doi.org/10.3126/stcj.v2i1.14793.

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The present study was undertaken to evaluate the microbiological quality of indigenous dahi from eastern Nepal. Atotal of 39 indigenous dahi samples were collected from sixteen districts of eastern Nepal and analyzed. Results revealed the mean yeasts and mould count to be 20.5×104±7503, coliform count 65±42, S. aureus 197±65 and total viable bacterial count 227×106±17250 cfu/g. Total viable bacteria and yeasts and moulds were present in all samples examined. Coliforms were present in 90 % and S. aureus were present in 63% of samples examined whereas salmonella species were not detected in any

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Yokomaku, Daisaku, Nobuyasu Yamaguchi, and Masao Nasu. "Improved Direct Viable Count Procedure for Quantitative Estimation of Bacterial Viability in Freshwater Environments." Applied and Environmental Microbiology 66, no. 12 (2000): 5544–48. http://dx.doi.org/10.1128/aem.66.12.5544-5548.2000.

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ABSTRACT A direct viable count (DVC) procedure was developed which clearly and easily discriminates the viability of bacterial cells. In this quantitative DVC (qDVC) procedure, viable cells are selectively lysed by spheroplast formation caused by incubation with antibiotics and glycine. This glycine effect leads to swollen cells with a very loose cell wall. The viable cells then are lysed easily by a single freeze-thaw treatment. The number of viable cells was obtained by subtracting the number of remaining cells after the qDVC procedure from the total cell number before the qDVC incubation. T

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Tiwari, Shveta, Swapnil Pradhan, Varsha Shukla, and Anushree Lokur. "Validation of Portable Culture Device for Enumerating Total Viable Count from Food Samples." Journal of Advanced Scientific Research 16, no. 02 (2025): 06–14. https://doi.org/10.55218/jasr.2025160202.

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Bacterial contamination in food arises from various sources, including human contact and processing equipment, and often worsens during storage, affecting food quality. Traditional bacterial enumeration methods, though reliable, are time-consuming, while newer alternatives like biosensors provide faster results but are more expensive. The accurate identification of microorganisms is critical for food safety in the food industry. Portable culture devices (PCDs) offer a practical solution for on-site testing, particularly in resource-limited settings. This study aimed to validate the use of PCDs

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